13 results about "Rhododendron molle" patented technology

The invention belongs to the field of biology, and particularly relates to a rhododendron molle heat shock protein RmHSP20-4 gene and application thereof. The RmHSP20-4 participates in response of rhododendron molle to high-temperature stress, and can be used for screening high-temperature tolerance germplasm of rhododendron molle and evaluating high-temperature tolerance of sheep. The gene is transferred into a prokaryotic expression strain, the growth of the transgenic strain under the stress of CdCl2 and high temperature is obviously improved, and the RmHSP20-4 gene and a protein coded by the RmHSP20-4 gene provide candidate genes for the cultivation of Rhododendron molle heavy metal resistance, high temperature stress resistance and other related varieties. A new candidate gene resource is provided for cultivating transgenic plants or microorganisms with excellent shapes such as heavy metal resistance and high temperature stress resistance, and an important reference is also provided for mining heat-resistant genes of azaleaceae plants.

Belonging to the technical field of insecticides, the invention discloses a sterilization insecticide, which comprises the following raw materials by weight: 20-40 parts of water; 0.1 to 0.5 part of herb of lindera glauca; 0.1 to 0.5 part of rhododendron molle; 0.2 to 0.7 part of Japanese raspberry root; 0.1 to 0.5 part of typhonium giganteum; and 0.1 to 0.5 part of Aconitum aureum. 0.1 to 0.5 part of pinellia ternate; 0.05 to 0.4 part of plant ash; 0.2 to 0.8 part of white spirit; according to the invention, the reagent with sterilizing and insecticidal effects can be obtained by rapidly proportioning water, herb of lindera glauca, rhododendron molle, root of Japanese raspberry, typhonium giganteum, root of aconitum var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var. Var

The invention belongs to the field of biology, and particularly relates to a rhododendron molle heat shock protein RmHSP20-5 gene and application thereof. The RmHSP20-5 gene participates in response of rhododendron molle to high-temperature stress, and can be used for screening high-temperature tolerance germplasm of rhododendron molle and evaluating high-temperature tolerance of sheep. When the gene is transferred into a prokaryotic expression strain, the growth of the transgenic strain under low-temperature stress, high-temperature stress, sodium chloride stress and cadmium chloride stress is obviously improved, and a new candidate gene resource is provided for cultivating transgenic plants or microorganisms with excellent shapes such as heavy metal resistance and high-temperature stress resistance; and an important reference is provided for mining of the heat-resistant gene of the azaleaceae plant.

The invention belongs to the field of plant tissue culture, and particularly relates to an in-vitro propagation method of Chinese herbal medicine rhododendron decidulum-rhododendron molle. The in-vitro propagation method comprises the steps of explant collection and treatment, adventitious bud differentiation induction, elongation and stem extraction, rooting induction and acclimatization and transplantation, wherein wild rhododendron molle branches are collected to cultivate tender leaves, and the tender leaves are washed and disinfected to obtain explants; and sequentially inoculating into WPM1, WPM2 and WPM3 culture mediums, respectively completing adventitious bud induction, stem drawing and rooting culture, and finally, carrying out acclimatization and transplanting to obtain adult plants. According to the method, tender leaves are used as explants, the culture medium is simple in formula, few in hormone variety, safe and environment-friendly to operate, high in propagation efficiency and short in period, the adventitious bud induction rate and the transplanting survival rate both reach 100%, the method is suitable for large-scale production, and technical support is provided for rhododendron molle species protection and Chinese herbal medicine industrialization.

This invention discloses a library of traditional Chinese medicine (TCM) compositions based on general and local anesthesia mechanisms, along with various dosage forms and preparation methods. The invention constructs a systematic library of TCM anesthetic compositions: the general anesthetic composition consists of extracts from herbs such as Datura stramonium, Datura stramonium, processed Aconitum carmichaelii, processed Aconitum kusnezoffii, Saussurea involucrata, Jasminum sambac root, Rhododendron molle, and Sanqie Sanqie; the local anesthetic composition consists of extracts from herbs such as Asarum heterotropoides, Murraya paniculata, Ephedra sinica, Toad venom, and Corydalis yanhusuo. This invention further develops the above compositions into various modern dosage forms, including injections, tablets, capsules, and sprays. In particular, this invention innovatively introduces a clove-cinnamon natural preservative and flavoring system to replace chemical preservatives. In terms of preparation technology, advanced technologies such as supercritical CO2 extraction, microwave-assisted extraction, nano-embedding, and nanoemulsions are integrated, and a self-extraction scheme is adopted to achieve controllable quality and cost. Simultaneously, a quality detection method based on HPLC fingerprinting is established. This invention achieves full-scenario coverage of anesthesia and analgesia, from general to local, from emergency to routine care, with outstanding advantages of safety, effectiveness, and controllable quality.

This invention belongs to the field of traditional Chinese medicine preparations, and relates to the preparation method and application of a compound preparation of Yao Yu effervescent tablets with anti-rheumatoid arthritis effect. The compound preparation comprises the following traditional Chinese medicine components: 4 parts by weight of Dioscorea cirrhosa, 3 parts by weight of Tinleaf Vine, 2 parts by weight of Camphor Tree, 2 parts by weight of Rhus chinensis, 3 parts by weight of Rhododendron molle, 1 part by weight of Ephedra sinica, 2 parts by weight of Semi-Acer palmatum, 2 parts by weight of Gynostemma pentaphyllum, and 1 part by weight of Cinnamomum cassia. The preparation steps include: placing Dioscorea cirrhosa, Tinleaf Vine, Camphor Tree, Rhus chinensis, Rhododendron molle, Gynostemma pentaphyllum, Semi-Acer palmatum, Gynostemma pentaphyllum, and Cinnamomum cassia in 6000 parts by weight of 70% ethanol. Extract for 2 hours, repeat three times, filter, and combine the filtrates; concentrate, freeze-dry, pulverize, and pass through an 80-mesh sieve for later use; mix 27.5% freeze-dried powder with 26.45% citric acid, 34.11% sodium bicarbonate, 5.61% PEG6000, and 6.33% lactose evenly, pass through an 80-mesh sieve, and prepare a soft mass using anhydrous ethanol as a wetting agent; pass the soft mass through a 20-mesh sieve and dry at 37℃ for 30 minutes to obtain compound preparation granules; granulate through a 20-mesh sieve for granulation, add lubricant PEG6000 and mix evenly; compress into tablets to obtain compound preparation effervescent tablets. This invention, through pharmacological experiments, confirms that the Yaoyu effervescent tablet compound preparation has a good anti-rheumatoid arthritis effect: it can reduce local inflammatory mediators and serum IL-1β, IL-6, TNF-α, and PEG2 levels, downregulate MDA and increase SOD to improve oxidative stress, inhibit the expression of MMP3, COX-2, and P38 proteins in the synovial membrane, and reduce pathological tissue damage. The medium and high dose groups showed the best effect, and it has a good therapeutic and protective effect on the ankle joint of AA rats, with good clinical application value.

The invention belongs to the technical field of medicines, and discloses an application of a rhododendron molle toxin VI derivative in analgesia. Specifically, the invention discloses a derivative shown as a formula I or a pharmacodynamically acceptable salt thereof, a pharmaceutical composition containing the derivative or the pharmacodynamically acceptable salt thereof, an application of the compound in preparation of a medicine for preventing or treating pain and an acute toxicity research of the compound.

The application discloses a Rhododendron molle flower extract with immunosuppressive activity and a preparation method and application thereof. The Rhododendron molle flower extract is prepared by taking dried flower powder of Rhododendron molle as raw material and 95% ethanol as an extracting agent under the following conditions: condensation reflux extraction for 2 hours at 60 DEG C and a material-liquid ratio of 1:10. The extract is filtered and collected, and the above extract is combined and concentrated under vacuum and reduced pressure to obtain the Rhododendron molle flower extract. The extract has an inhibitory rate of 61.41% on T lymphocyte proliferation induced by abrin and an inhibitory rate of 51.54% on B lymphocyte proliferation induced by lipopolysaccharide at a concentration of 100 mg / mL, and has no cytotoxicity on normal mouse spleen lymphocytes. The application provides a candidate research material for researching and developing a new immunosuppressant, and provides a scientific basis for developing and utilizing the Rhododendron molle resources.

The invention discloses a method for realizing transient expression of exogenous target genes in rhododendron molle plants by using a vacuum agrobacterium infection method, and belongs to the technical field of forest tree genetic engineering. The method comprises the following steps: performing ultrasonic pretreatment on rhododendron molle seedlings, immersing the plants into an agrobacterium infection solution containing a target gene, performing circulating vacuum treatment with specific parameters to enable the infection solution to efficiently permeate into tissues, and finally performing shading and normal culture to complete transient expression. By optimizing the ultrasonic treatment conditions and combining the circulating negative pressure vacuum infiltration technology, the problems that a traditional injection method is low in efficiency, tedious in operation and incapable of achieving batch treatment are solved. According to the method, the conversion efficiency and expression uniformity of the exogenous gene at the whole rhododendron molle plant level are remarkably improved, the operation is simple and convenient, the period is short, the repeatability is good, and an efficient and reliable technical platform is provided for gene function research and molecular breeding of rhododendron molle and rhododendron plants.

The invention belongs to the technical field of medicines, and discloses xyleveratrane diterpenes, a preparation method thereof, a pharmaceutical composition thereof and an application of the xyleveratrane diterpenes. In particular relates to an analgesic active ingredient separated from rhododendron molle G.Don. Flower, a novel xyletre diterpenoid compound 1 and pharmacodynamically acceptable salt thereof, a preparation method of the novel xyletre diterpenoid compound 1, a composition containing the novel xyletre diterpenoid compound 1, an application of the novel xyletre diterpenoid compound 1 in preparation of analgesic drugs and an acute toxicity research of the novel xyletre diterpenoid compound 1 in preparation of the analgesic drugs.

The invention relates to the technical field of preparation of health-care products, and provides a compound foot bath tablet as well as a preparation method and application thereof. The compound foot bath tablet comprises the following components: a monarch drug, a ministerial drug, an adjuvant drug, a conductant drug and auxiliary materials. Wherein the monarch drug comprises a rhodiola rosea extract, safflower, a lucid ganoderma extract, sea-buckthorn and crocus sativus salt; the ministerial drugs comprise fructus chebulae extract, fructus terminaliae billericae extract, emblic leafflower fruit extract, fructus schizandrae extract, radix codonopsis, radix salviae miltiorrhizae, herba leonuri, radix notoginseng, juniperus formosana, rhizoma kaempferiae extract, radix astragali seu hedysari, myricaria laxiflora, rhizoma chuanxiong, sea salt, herba artemisiae annuae, lavender and mirabilis jalapa extract; the adjuvant drugs comprise gentiana macrophylla extract, gentian extract, rhizoma bolbostemmae extract, divaricate saposhnikovia root, citrus peel, sappan wood, perilla leaf, herba lycopi extract, cowherb seed extract, tribulus terrestris, rhododendron and rheum palmatum; the conductant drug comprises gastrodia elata and calamus; the auxiliary materials comprise an effervescing agent, a disintegrating agent and an adhesive. The compound foot bath tablet can clear damp and help sleep, and is convenient to use, safe and reliable.

The invention belongs to the field of biology, and particularly relates to a rhododendron molle heat shock protein RmHSP20-1 gene and application thereof. The rhododendron molle small heat shock protein RmHSP20-1 gene participates in response of rhododendron molle to high-temperature stress, and can be used for screening high-temperature tolerance germplasm of the rhododendron molle and evaluating the high-temperature tolerance of the rhododendron molle. Candidate genes are provided for cultivation of Rhododendron molle anti-heavy metal, salt, high and low temperature stress and other related varieties. The gene can also be used as an important gene resource, is applied to stress resistance and heavy metal stress resistance gene engineering of other plants or microorganisms except rhododendron molle, and provides a new candidate gene resource for cultivating transgenic plants or microorganisms with excellent characters of heavy metal resistance, salt resistance, high and low temperature stress resistance and the like. And an important reference is provided for mining of the heat-resistant gene of the azaleaceae plant.

The application discloses a method for rapidly propagating aseptic seedlings by using flower buds of Asystasiarugosa, selects Asystasiarugosa plants which are healthy and free from diseases and pests, collects flower buds, disinfects, peels off the outer scales of the flower buds, cuts the inner tissues of the flower buds by using a sterile scalpel, inoculates into pre-culture medium and carries out culture; then carries out differentiation culture, subculture, proliferation culture, rooting culture, seedling raising and transplanting to obtain Asystasiarugosa aseptic plants, solves the problems that disinfection is difficult to be thorough, is easy to be polluted and primary aseptic seedlings are difficult to be obtained when stems and leaves of Asystasiarugosa are used as explants to carry out tissue culture, provides a new way for rapidly propagating Asystasiarugosa aseptic seedlings, and creates favorable conditions for Asystasiarugosa breeding and genetic transformation research. The application uses flower buds of Asystasiarugosa as explants, can obtain a large number of aseptic plants in a short time, has a high propagation coefficient, and is favorable for carrying out Asystasiarugosa breeding research and enterprise standardized seedling raising.