85results about How to "Efficient expression" patented technology

The invention provides a method for constructing a chemical-plastic industry chain knowledge graph by using a graph convolutional network, and relates to the technical field of knowledge graphs, and the method comprises the steps: obtaining the multi-source heterogeneous data of a chemical-plastic industry chain through a data collection system, and forming an initial data set; preprocessing the initial data set to generate a standardized data set; performing knowledge modeling based on the standardized data set to form a domain knowledge model; constructing an initial knowledge graph according to the domain knowledge model; and optimizing the initial knowledge graph by using a graph convolutional network, including constructing a graph convolutional network model, training the model, and performing automatic completion and error correction on the knowledge graph through the trained model to obtain an optimized chemical and plastic industry chain knowledge graph. Through systematic data processing, knowledge modeling and graph convolutional network optimization methods, efficient expression, automatic complementation and error correction of the knowledge of the chemical and plastic industry chain are realized, so that an accurate and reliable chemical and plastic industry chain knowledge graph with an automatic optimization capability is constructed.

The embodiment of the invention discloses a three-dimensional reconstruction method based on structured three-dimensional Gaussian representation. The method comprises the following steps: acquiring a target image containing a target object at multiple visual angles; generating an unstructured Gaussian primitive representation corresponding to the target object according to the target image; establishing a transmission matrix describing the unstructured Gaussian primitive distribution and the structured Gaussian primitive distribution, solving the transmission matrix by using an optimal transmission algorithm, and generating a structured Gaussian primitive representation corresponding to the target object according to the optimal transmission matrix; obtaining a text description for the target object, and complementing the structured Gaussian primitives according to the text description by using a diffusion model obtained by pre-training; and performing three-dimensional reconstruction by using the complete structured Gaussian primitive. According to the method, the optimal transmission algorithm is used for carrying out structured representation on the three-dimensional Gaussian primitives, and text description is used for complementing the Gaussian primitives through the diffusion model, so that the quality of three-dimensional reconstruction is improved.

The invention belongs to the field of biological medicines, and particularly relates to a modified double-stranded oligonucleotide, and a conjugate and a composition thereof. The double-stranded oligonucleotide provided by the invention effectively enhances the activity of the double-stranded oligonucleotide for inhibiting the expression of the target gene by performing specific modification on the nucleotide at a specific site, can efficiently inhibit the expression of the target gene in both in-vitro and in-vivo experiments, and surprisingly, the double-stranded oligonucleotide provided by the invention has a good application prospect. The modification on the double-stranded oligonucleotide can be generally used for siRNA designed for various different target genes, and the inhibition rate of the siRNA on the target genes can be effectively improved.

The invention discloses a drug target combinability prediction method based on variational coding, and belongs to the field of bioinformatics. According to the method, efficient extraction of drug and target features, construction of potential feature space of the drug and target features and prediction of the interaction relationship of the drug and target features are realized through a dual-stage coupled end-to-end neural network. The model is mainly composed of two stages, in the first stage, potential representations of drug and target features are obtained through variational compression and feature extraction, and in the second stage, a drug target action matrix is reconstructed through an up-down sampling comprehensive deep neural network. Through the architecture, the chemical characteristics of the drug and the biological characteristics of the target can be effectively integrated, and the accuracy of interactive relationship prediction is improved. The invention provides a novel and efficient solution for deep analysis of biological big data at the same time.

The invention belongs to the technical field of gene engineering and biology, discloses a modified adeno-associated virus AAV-8 serotype for retina wide targeting and expression, and further relates to construction and application of the modified type. 10 amino acid sequences as shown in SEQ ID NO.3 are inserted between the 590th amino acid and the 591th amino acid of an AAV-8 serotype capsid protein sequence as shown in SEQ ID NO.2, the first, second and tenth amino acids are protected amino acids, and the third to ninth amino acids are amino acid sequences obtained by screening. The modified serotype disclosed by the invention is stronger in fluorescence effect after infection and can be obviously and intuitively observed, an exogenous gene can be widely and efficiently expressed in a specific targeted tissue retina whole region, and the expression efficiency is greatly improved under the condition of using the same virus amount. And compared with a wild serotype, the serotype has better and wider targeted tissue expression and less non-targeted tissue expression.

The invention belongs to the field of biomedical materials, and particularly relates to a PBAE-DOPE-PEG2000 polymer-lipid nanocomposite and a preparation method and application thereof.The preparation method comprises the steps that a dimethyl sulfoxide-ethanol solution formed by mixing DOPE-PEG2000 lipid and PBAE serves as an oil phase, a sodium acetate / acetic acid buffer solution serves as a water phase, and the PBAE-DOPE-PEG2000 polymer-lipid nanocomposite is prepared through a microfluidic synthesis mode; the PBAE-DOPE-PEG2000 polymer-lipid nano composite material is prepared by the steps of carrying out ultrasonic treatment on the PBAE-DOPE-PEG The polymer-lipid nano composite material can efficiently load exogenous genes, the surface performance of the polymer-lipid nano composite material is changed and the stability of nano particles is improved by introducing DOPE-PEG2000 lipid, membrane fusion can be promoted, the risk of lysosome degradation is reduced, the transfection process of the exogenous genes in recipient cells is accelerated, and efficient expression in 293FT cells is realized. The efficient transfection of the polymer-lipid nanocomposite material is expected to show important application prospects in immune cell therapy or tumor cell therapy.

The invention discloses coprinus cinereus sourced laccase as well as a recombinant expression strain and a high-efficiency expression method thereof, and belongs to the technical field of microbiology and bioengineering. According to the invention, aspergillus niger MA70.15 is used as an experimental strain, and a random integration strategy is adopted, so that a pC3-PcitA-SPGlaA-lcc9-TtrpC-pyrG expression vector is integrated in an upstream region of a promoter of host bacteria citrate synthase in a form of nine continuous copies. In order to explore the influence of particles and osmotic regulators in a fermentation culture medium on laccase production through fermentation of recombinant aspergillus niger, the particles and osmotic regulators of different types and concentrations are added, so that the enzyme activity of the laccase fermented in a shake flask of the recombinant strain AnRcgL1 reaches 1566.7 U / L, the enzyme activity fermented in a 1L fermentation tank reaches 1961 U / L, and the recombinant high-efficiency expression of the laccase Lcc9 in the aspergillus niger is successfully realized.

The invention provides an airborne point cloud classification method and system considering a global-local self-attention mechanism. The method comprises the steps of 1, selecting an ISPR-3D airborne point cloud data set; 2, preprocessing the point cloud data; step 3, constructing an edge convolutional network based on the point cloud data, and extracting local features of the point cloud; 4, constructing a local self-attention mechanism network based on the extracted local features of the point cloud; step 5, constructing a global self-attention mechanism network; step 6, constructing a global-local self-attention mechanism module; 7, adopting a U-net network design to complete an overall network structure; and step 8, inputting airborne laser point cloud test data to be classified into the trained overall network structure to obtain a category label of each point. The invention innovatively provides a global-local self-attention mechanism neural network integrating edge convolution for solving the problems of insufficient feature expression and unbalanced sample categories in airborne point cloud large scene classification.

The invention relates to a strong promoter P7 suitable for streptomyces and application thereof, and relates to the field of genetic engineering and microbial metabolism engineering. The nucleotide sequence of the strong promoter P7 is as shown in SEQ ID No.1, and the strong promoter comprises a plasmid vector of the strong promoter; a host cell comprising the plasmid vector; the invention also discloses application of the strong promoter, the plasmid vector and the host cell in starting expression of a target gene. Compared with the prior art, the characterization of the strong promoter P7 provides an effective tool element for streptomyces strong promoter engineering and high-efficiency gene expression, and has important significance on streptomyces silent gene characterization, high-efficiency gene expression, metabolite synthesis, metabolic pathway reconstruction and the like. The strong promoter can be applied to common streptomyces type strains, and has important significance on high yield of important proteins including enzymes and important metabolites from actinomycetes.

The invention discloses a cartilage repair hydrogel material and a preparation method thereof, and relates to the technical field of biomedical materials. The preparation method comprises the following steps: adding a vitamin E water-soluble derivative D-alpha-tocopherol polyethylene glycol 1000 succinate, T-ZIF-8 modified by 2, 4-toluene diisocyanate and Mg-MOF modified by SIKVAV peptide into a mixed solution obtained by compounding silk fibroin peptide, oxidized chondroitin sulfate and 3-aminophenylboronic acid, and carrying out crosslinking under ultraviolet light, so as to obtain the cartilage repair hydrogel material. The hydrogel material prepared by the method has a three-dimensional complex cross-linked structure and is high in mechanical property and stability; through the synergistic effect of the components, an adhesion group and a hydrophilic group are created for the surface of the hydrogel material, the binding opportunity of bone cells and the surface of the hydrogel material is increased, adhesion and proliferation of the bone cells are promoted, rejection reaction of tissues to metal materials is reduced, inflammatory reaction is reduced, and collagen secretion and cartilage regeneration are promoted.

The invention discloses recombinant hansenula polymorpha for efficiently producing cat serum albumin and an expression method of the recombinant hansenula polymorpha. According to the method, an expression cassette of the cat serum albumin is integrated in hansenula polymorpha, and a promoter in hansenula polymorpha is optimized, so that the serum albumin is expressed by a constitutive promoter and a sucrose inducible promoter independently or in a combined manner, and the copy number of the serum albumin is further optimized; the stable and efficient expression of the cat serum albumin gene in hansenula polymorpha is successfully realized. The high-density fermentation yield of the recombinant cat serum albumin fermented by using the engineering bacteria can reach 1.7-2.2 g / L.

The invention discloses a power distribution network operation multi-target optimization scheduling method and system, and solves the problem that the existing method cannot decompose and predict high-frequency and low-frequency sequence trends of target control parameters such as wind and light power generation from the perspective of high-frequency fluctuation and low-frequency trends of the control parameters. The method comprises the following steps: acquiring target point load and wind and light power generation output parameters in the power distribution network; summing the intra-day low-frequency prediction sequence and the intra-day high-frequency prediction sequence of the target point, generating an intra-day demand response curve of the target point by the bidirectional long-short-term memory network model, and outputting a multi-target optimization scheduling scheme based on the demand response curve and the power distribution cost prediction sequence; according to the method, the power distribution cost sequence parameters are decomposed by adopting the variational mode decomposition method, so that the high-frequency cost sequence and the low-frequency cost sequence of each target point are quickly and efficiently obtained, the complexity of the load sequence is remarkably reduced, and accumulated errors during prediction are reduced.

The present disclosure relates to the technical field of computers, in particular to a method and apparatus for classifying a blockchain address. The method includes: transaction vectors are generated for transaction records corresponding to each blockchain address in a blockchain address set; a corresponding address vector is generated based on the transaction vectors corresponding to a blockchain address to be classified; and the address vector of the blockchain address to be classified is input into a classifier, so as to determine a class of the blockchain address to be classified. By means of the method, equal-length vectors representing the blockchain addresses can be obtained for classification by the classifier, transaction behaviors and association relationships of participating entities in a blockchain are effectively expressed. At the same time, the problem that feature engineering in traditional machine learning is prone to being affected by the external environment is also avoided, the classification accuracy is high, and a result is reliable; and the defects of error propagation, insufficient algorithm generalization, high requirements for hardware resources and time costs, etc. in existing methods are also overcome.

This invention discloses a stable transfected cell line for glucocorticoid detection based on the Gal4-DBD-GRLBD fusion protein of marine medaka and its construction method, belonging to the field of environmental toxicology detection technology. The construction method includes: designing specific primers to amplify the GR ligand-binding domain (LBD) of marine medaka, ligating it to the pBIND vector to form the coding sequence of the Gal4-DBD-GRLBD fusion protein, subcloning it into the Dualpromoter vector to construct the pBIND-omeGR expression plasmid; co-transfecting HeLa cells with the pBIND-omeGR plasmid, UAS reporter plasmid, and transposase plasmid at a mass ratio of 1.66:1:0.34, and obtaining the stable cell line HeLa-pBIND-omeGR expressing the fusion protein after selection with puromycin. The cell line exhibits ECMO activity against dexamethasone. 50 The value was 0.841 nM, and the induction activation factor was 2.34 times. This invention solves the technical problems of existing mammalian GR detection systems, which are difficult to accurately predict fish toxicity effects and have complex construction of full-length receptor systems. It has the advantages of simple construction, strong versatility, and good stability, and can be used for marine ecotoxicological assessment, environmental pollutant monitoring, and chemical safety evaluation.

The invention discloses a bacillus subtilis genome integrated plasmid for efficient and stable expression of genes and application of the bacillus subtilis genome integrated plasmid in production of beta-galactosidase. The plasmid comprises a bacillus subtilis promoter, an RBS sequence, a terminator, a bacillus subtilis resistance gene, a genome integration homologous arm, an escherichia coli replicon and a resistance gene. The homologous arm is selected from upstream and downstream sequences of sites behind termination codons such as genes spxA, tatCY, rlmCD and the like, so that exogenous genes are accurately integrated to a plurality of non-essential sites on the premise of not damaging essential genes. The invention also provides a method for constructing a bacillus subtilis engineering strain for producing beta-galactosidase by using the plasmid. The integration system is high in expression level, stable expression can be achieved without adding antibiotics and inducers, the fermentation cost is remarkably reduced, the enzyme production efficiency is improved, and the integration system is suitable for industrial enzyme preparation production.

The invention discloses an eurytopic chlamydomonas reinhardtii expression vector based on a PSAD promoter and a 3 * Flag tag as well as a construction method and application of the eurytopic chlamydomonas reinhardtii expression vector. The nucleotide sequence of the eurytopic chlamydomonas reinhardtii expression vector is shown as SEQ ID No.1; the vector comprises a PSAD promoter, a target gene insertion site EcoR V, a 3 * Flag tag at the 3'terminal, an rbcS2 terminator, a TUB2 promoter, a hygromycin B screening gene and an rbcS2 terminator. The construction method comprises the following steps: constructing a skeleton of a pHyg-PSAD-3Flag vector; constructing a sequence fragment fused with a 3 * Flag tag; and connecting the sequence fragment fused with the 3 * Flag tag with a plasmid skeleton fragment through T4 DNA (deoxyribonucleic acid) enzyme so as to construct the pHyg-PSAD-3Flag vector. According to the invention, the expression plasmid of the chlamydomonas pHyg-PSAD-3Flag can be used for efficiently screening algae strains capable of stably expressing target protein.

The invention discloses cryptocaryon irritans inhibition antigen bigeminy recombinant protein as well as a preparation method and application thereof. The amino acid sequence of the cryptocaryon irritans inhibition antigen bigeminy recombinant protein is as shown in SEQ ID NO: 1. By means of terminator replacement and codon optimization and insertion of the Linker protein of the 15 peptide composed of Gly and Ser between the two activity-inhibiting antigen proteins, the situation that the two activity-inhibiting antigen recombinant proteins possibly interfere with each other in the spatial structure, and the integrity of the antigen epitopes of the two activity-inhibiting antigen recombinant proteins is affected is avoided; the defect that a single inhibition antigen provides immune protection for cryptocaryon irritans infection is overcome. The cryptocaryon irritans inhibition antigen bigeminy recombinant protein can be used for preparing vaccines, medicines or immune preparations for preventing and treating cryptocaryon irritans diseases.

The application relates to the technical field of marketing, and discloses a marketing management method and system based on big data, which comprises a dynamic situation sensing and demand prediction module: potential consumption demand is predicted based on authorized multi-source data; an active situation declaration and solution generation module: structured life task declarations actively input by users are received, and a solution package is generated; an individual utility preview and verification module: a product is subjected to multi-physical field simulation and multi-dimensional adaptability evaluation; a closed-loop feedback and ethical guarantee module: the system is continuously optimized and data compliance is guaranteed. Through the cooperative operation of the modules, the demand identification mode can be optimized, the marketing precision can be improved in combination with a double path, invalid guidance can be reduced, the user interaction experience can be improved, the decision cost can be reduced, the cold start problem can be alleviated, data compliance can be guaranteed through a multiple privacy protection mechanism, user trust can be enhanced, long-term iterative optimization of the system can be realized, and operation stability can be guaranteed.

The invention belongs to the field of plasmid vector construction, and provides a plasmid vector as well as a construction method and application thereof. The plasmid vector is constructed by modifying pAN7-1, a trichoderma viride promoter gene and a resistance gene are inserted into multiple cloning sites of the pAN7-1 of the plasmid vector, and the resistance gene is located at the downstream of the trichoderma viride promoter gene. According to the invention, the selected trichoderma viride promoter gene segment can efficiently start the gene connected behind, so that the transformation efficiency of the plasmid vector is greatly improved. The invention establishes a method for efficiently transforming trichoderma viride by using an agrobacterium tumefaciens-mediated exogenous gene, and can provide help in the aspects of genetic modification of trichoderma viride, development of useful genes, separation of drug target genes and the like.

The invention discloses an in-vitro culture and separation method of an NK cell exosome loaded with a PD-1 antibody, and relates to the technical field of biomedicine, and the in-vitro culture and separation method specifically comprises the following steps: (1) culturing an NK cell; (2) loading a PD-1 antibody; (3) exosome extraction; (4) purifying the exosome; efficient loading of the PD-1 antibody is realized through electroporation, high expression of the antibody in the exosome is ensured, and large-scale production of the exosome is ensured through optimized NK cell culture conditions and an exosome extraction process; inorganic particles are added in cell culture to serve as microcarriers, more attachment points are provided for cells, transmission of nutrient substances and removal of metabolic waste are optimized, the proliferation multiple of the cells is increased, the cell viability is kept, the overall loading efficiency and growth amount of the microcarriers are increased, and therefore growth and proliferation of the NK cells are promoted; the large-scale culture of the cells is realized.

The application belongs to the technical field of genetic engineering, and particularly relates to a method for establishing a plant line of an influenza B virus HA protein expressed by Nicotiana benthamiana. According to the method, a nucleotide sequence coding the BV-HA protein is first connected to a plant binary expression vector pHREAC to construct a recombinant plasmid, then the recombinant plasmid is transformed into an E. coli competent cell, an Agrobacterium competent cell in sequence, and the Agrobacterium competent cell is used to infect the Nicotiana benthamiana, so that the BV-HA protein is obtained through transient expression in the Nicotiana benthamiana cell. The BV-HA protein purified through the method has high biological activity, and can be used for further biological function research. The method can realize one-step purification of the protein, is simple and fast, and has high protein purity. The exogenous recombinant protein obtained through the method can be used for biological safety evaluation and biological function research.

The invention provides a GFAP recombinant protein, a monoclonal antibody and a preparation method and application thereof. The invention realizes efficient, soluble and active expression of GFAP in Escherichia coli by optimizing antigen peptide sequences and expression vectors. Mouse monoclonal antibodies specifically binding to GFAP protein are screened based on the GFAP recombinant protein, and paired monoclonal antibodies are screened using the mouse monoclonal antibodies. An ELISA detection method or kit for double antibody sandwich immunization and biotin-anti-biotin monoclonal antibody amplification applied to GFAP is further constructed based on the monoclonal antibody combination. The method or kit can specifically detect human GFAP protein, has no cross reaction with other neural markers S-100B protein and neuron-specific enolase (NSE), is consistent with the detection value of a fixed-value calibrator, and has high detection sensitivity.

An RNA delivery system for the treatment of Huntington's disease. The system comprises a viral vector, the viral vector carries RNA fragments capable of treating Huntington's disease, the viral vector is capable of enrichment in organ tissues of a host and endogenously and spontaneously forming a complex containing the RNA fragments capable of treating Huntington's disease in the organ tissues of the host, and the complex can deliver the RNA fragments into a target tissue to treat Huntington's disease. The safety and reliability of the RNA delivery system for the treatment of Huntington's disease have been fully verified. The system has good medicinal properties, strong versatility, and has economic benefits and application prospects.

The invention belongs to the technical field of gene engineering, and discloses a pGL-FOXP3 promptor-reporter-FOXP3 enhancer plasmid capable of effectively detecting the expression of human FOXP3 in a cell line, and construction and application of a drug screening system of the pGL-FOXP3 promptor-reporter-FOXP3 enhancer plasmid. The pGL-FOXP3 promptor-reporter-FOXP3 enhancer plasmid can be effectively applied to the expression of FOXP3 in a human Jurkat cell and a human 293 cell, the research on related pathways and the drug screening. A 293 cell FOXP3 expression increasing or reducing model stimulated by different conditions of PMA or TNF-alpha is constructed for the first time and can be applied to drug screening with an FOXP3 expression regulating effect, and biological tool support is provided for research of subsequent drug animal experiments and clinical experiments. In addition, candidate drugs MFA-1 and MTX capable of reducing FOXP3 expression and a candidate drug EIE-2 capable of bidirectionally adjusting FOXP3 balance are screened and obtained by the inventor in experiments, and the FOXP3 balance adjusting effect of the EIE-2 is verified in rheumatoid arthritis animal experiments (data are not disclosed).

This invention provides a method and application for transient expression of collagen in plants. The collagen fragment C3S1, C3S2, C3S3, C3S4, and C3S5 are linked together with the amino acid sequences shown in SEQ1, SEQ2, SEQ3, SEQ4, and SEQ5, respectively. This collagen fragment can be expressed using tobacco as a bioreactor. By designing expression vectors, its expression effect in tobacco can be compared, and a transient expression system in tobacco can be established to achieve efficient expression of the collagen fragment in tobacco. This preparation method can express recombinant collagen fragments in tobacco with high expression levels. Compared to full-length intron-modified collagen or expression using type I collagen, this method achieves efficient expression of type III collagen fragments in tobacco bioreactors. It has a short cycle time, requires no maintenance of cell lines and bioreactors, significantly reduces production costs, and has a high safety index.

This invention belongs to the field of biomedical technology and discloses a chimeric antigen receptor targeting CD20, its gene expression construct, and its applications. The chimeric antigen receptor provided by this invention specifically includes an amino acid fragment with the sequence shown in SEQ ID NO:29 or a variant fragment having at least 85% sequence identity with SEQ ID NO:29. Its functional fragments, including the signal peptide, antigen-binding region, hinge region, transmembrane region, intracellular co-stimulatory domain, and signal transduction domain, form an organic whole, working synergistically to endow the chimeric antigen receptor with good CD20-targeting binding, T-cell activation, and cell-killing effects, demonstrating excellent application potential in the preparation of drugs for the treatment of relapsed / refractory B-cell malignancies.

This invention discloses an amidase gene for degrading amide alcohol antibiotics and amide herbicides. right here The amidase gene, along with its encoded protein and applications, has the nucleotide sequence SEQ ID NO.1, a full length of 912 bp, encoding 303 amino acids, and its amino acid sequence is SEQ ID NO.2. This invention relates to the gene... right here This is the first publicly disclosed amidase gene for amide alcohol antibiotics cloned from the genome of a single isolated strain. The amidase encoded by this gene can not only hydrolyze the amide bonds of amide alcohol antibiotics, but also degrade various amide chemical herbicides such as propargite, bromoxynil, chlorpyrifos, and chlorobromopropylate. The amidase encoded by this gene, as well as genetically engineered bacteria containing the amidase gene, have very important theoretical and applied value in the degradation of amide alcohol antibiotic pollutants in aquatic environments such as sewage treatment plants and livestock and poultry breeding wastewater, and in the remediation of herbicide-contaminated soil.

The present invention relates to a thermophilic tennis beta-mannanase mutant with improved thermal stability and its application, a mutant with improved thermal stability is obtained by random mutagenesis and expressed in Bacillus subtilis. The results show that the optimum temperature of the β-mannanase mutant is 80 ° C, the optimum pH is 5.0, the shake flask fermentation is 4 days, and the konjac gum is used as the substrate, which is 4.1% higher than the wild-type enzyme activity; its half-life at 80 ° C is 5 h, which is 2 h more than the wild-type, and the enzyme is completely inactivated for more than 48 h. In addition, the ability of the β-mannanase mutant to hydrolyze locust bean gum and guar gum is 10.2% and 2.0% higher than the wild-type, respectively. The enzyme has shown good application prospects in the fields of oil extraction and animal breeding.

The invention discloses a method for constructing yarrowia lipolytica engineering bacteria and producing itaconic acid, which comprises the following steps: adopting an optimized heterologous expression strategy, utilizing mitochondrial targeting signal peptide to improve the positioning efficiency of a core enzyme Cis-acetic acid decarboxylase, enhancing the contact between the enzyme and a substrate and promoting the synthesis of itaconic acid, and then, utilizing mitochondrial targeting signal peptide to enhance the reaction between the enzyme and the substrate to promote the synthesis of itaconic acid. Glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase of escherichia coli are added, cofactor circulation in mitochondria is regulated and controlled, the generation and regeneration efficiency of NADPH and NADH is improved, finally, a transmembrane transporter gene ldhT is modified, the amino acid sequence and the spiral structure of the transmembrane transporter gene ldhT are optimized, the excretion efficiency of itaconic acid is improved, and the yield of itaconic acid is increased. The negative influence of intracellular accumulation on the cells is reduced.

The application discloses a recombinant carrier for preparing HLA / beta 2M complex and a preparation method and application thereof, relates to the technical field of antibody preparation of MHC-polypeptide complex, and provides a recombinant carrier for preparing HLA / beta 2M complex, wherein FOS leucine zipper bZIP motif is inserted between NheI / AvrII enzyme cutting sites of an original carrier, JUN leucine zipper bZIP motif is inserted between BsiWI / BlpI enzyme cutting sites, beta 2M gene sequence is inserted between AgeI / NheI enzyme cutting sites, and HLA sequence is inserted between specific enzyme cutting sites, so that the prepared HLA / beta 2M complex has high expression, and the highest expression reaches 92.45 mg / L.