4results about How to "Efficient expression" patented technology

This invention provides a method and application for transient expression of collagen in plants. The collagen fragment C3S1, C3S2, C3S3, C3S4, and C3S5 are linked together with the amino acid sequences shown in SEQ1, SEQ2, SEQ3, SEQ4, and SEQ5, respectively. This collagen fragment can be expressed using tobacco as a bioreactor. By designing expression vectors, its expression effect in tobacco can be compared, and a transient expression system in tobacco can be established to achieve efficient expression of the collagen fragment in tobacco. This preparation method can express recombinant collagen fragments in tobacco with high expression levels. Compared to full-length intron-modified collagen or expression using type I collagen, this method achieves efficient expression of type III collagen fragments in tobacco bioreactors. It has a short cycle time, requires no maintenance of cell lines and bioreactors, significantly reduces production costs, and has a high safety index.

The application discloses an enhancer regulatory element for transcriptional regulation and expression of a human LTF gene and application thereof, wherein the sequence of the regulatory element is SEQ ID No. 1. Experiments prove that the human-specific regulatory element can significantly activate the expression of a reporter gene (up to 14.93 times) in a bovine mammary epithelial cell line (MACT), breaks through the limitation of a species-conserved element in the prior art, and an expression system designed based on the element can realize efficient expression of LTF at a human level, thereby providing a new tool for mammary gland bioreactors and development of functional dairy products.

This invention relates to the field of antimicrobial peptide technology, and particularly to the antimicrobial peptide Ple-AB, its preparation method, and its applications. Using the fungal defensin Plectasin as a template, this invention designs and modifies the antimicrobial peptide Ple-AB. Compared with Plectasin, this antimicrobial peptide exhibits significantly higher antimicrobial activity and better bactericidal effects against Gram-positive bacteria; it also shows higher stability, particularly excellent resistance to trypsin; and its fermentation expression level is significantly improved. Furthermore, this antimicrobial peptide has very low hemolytic activity and cytotoxicity, making it suitable for development in antimicrobial drugs, additives, cosmetics, health products, and other fields, with broad application value and market prospects.

This invention provides recombinant humanized elastin, its preparation method, and its applications, relating to the field of bioengineering technology. Based on humanized elastin, this invention utilizes a combination design based on the functional domains of elastin to obtain recombinant humanized elastin, discovering novel bioactive functional fragments of humanized elastin. The recombinant humanized elastin of this invention integrates multiple elastin functional sites. Specifically, this recombinant humanized elastin exhibits significant effects in promoting cell proliferation and increasing the expression of collagen and elastin genes, demonstrating high safety and wide applicability in various scenarios, and providing a new active ingredient for anti-aging related products. Furthermore, the theoretical molecular weight of this recombinant humanized elastin is significantly smaller than that of natural full-length elastin, reducing obstacles for elastin to cross the skin barrier and exert its biological functions, thus exhibiting excellent biological efficacy.