The invention discloses an chimeric antigen receptor gene for HIV-1. The gene comprises a guide subregion, a structural domain, a hinge region, a trans-membrane structural domain, a co-stimulation structural domain, a signal transduction structural domain and a suicide gene system region, wherein the structural domain is combined with a CD4 binding site of a gp120. The invention further disclosesa plasmid with the gene, a T cell, a kit and application. According to the chimeric antigen receptor gene for the HIV-1, the plasmid with the gene, the T cell, the kit and the application, when the Tcell is applied, HIV-1 infection can be prevented, and meanwhile, the lethality on an HIV-1 infected cell can be improved; and once an HIV-1-CAR-T cell is input, adverse reactions occur, the cell in abody can be cleared at once through a suicide gene system, and therefore the preventive effect can be achieved.