77results about How to "Low homology" patented technology

The invention relates to a pathogenic microorganism DNA detection chip and the chip comprises a carrier and a nucleic acid probe on the carrier. The nucleic acid probe is provided with a target detection probe used for detecting target gene of pathogenic microorganism. The pathogenic microorganism comprises but not limits to vibrio cholerae, pathogenic escherichia coli, campylobacter jejuni, Yersinia enterocolitica, parahemolytic vibrio, salmonella, and shigella and Listeria monocytogenes. The invention also relates to a preparation method of the pathogenic microorganism DNA detection chip and provides applications of the pathogenic microorganism DNA detection chip in detection of pathogenic microorganism. Also a pathogenic microorganism DNA detection chip kit is provided.

Recombinant pox viruses capable of expressing an immunogenic fragment of the MUC1 tumor-associated antigen are disclosed. The recombinant viruses can be used as vaccines to prevent the establishment of or treat tumors or pre-tumorous cells expressing the MUC1 tumor-associated antigen. The vaccines can be provided as an admixture comprising: (1) a recombinant pox virus encoding the immunogenic fragment of the MUC1 tumor-associated antigen, and (2) a recombinant pox virus encoding a T-cell co-stimulatory factor. The vaccine admixture can be used, e.g., to prevent establishment of tumors or pre-tumorous cells expressing the MUC1 tumor-associated antigen. The MUC1 specific cytotoxic T-cells can be isolated and expanded and used in a method for treating a host having a tumor expressing MCU1 positive tumor cells.

The present invention provides recombinant lentiviral vectors and gene transfer systems which produce said vectors, cell lines utilized in the production of said recombinant lentiviral vectors, and Bovine Immunodeficiency Virus DNA sequences utilized in the recombinant vectors and gene transfer systems.

The invention provides a detection primer set, probe set and detection reagent kit for novel coronavirus 2019. The detection primer set for novel coronavirus 2019 comprises the following primer pairs,including specific primer pairs of S genetic fragments for the novel coronavirus 2019, specific primer pairs of ORF1ab genetic fragments for the novel coronavirus 2019, and specific primer pairs of Ngenetic fragments for the novel coronavirus 2019. Through design of three pairs of specific primers, detection accuracy and precision for the novel coronavirus 2019 are improved.

The invention discloses a multiple RT-PCR primer group for simultaneously detecting porcine gatahvirus (GETV), porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV) and porcine A rotaviruses (PoRV), which has a nucleotide sequence as shown in SEQ ID NO:1-SEQ ID NO:10. The invention further discloses a multiple RT-PCR detection method for detecting GETV, PEDV, TGEV, PDCoV and PoRV from a sample in one time by utilizing the multiple RT-PCR primer group. Compared with an existing conventional RT-PCR, the detection method has strong specificity and high sensitivity, can realizesimultaneous identification of five viruses including GETV, PEDV, TGEV, PDCoV and PoRV, and has accurate detection result and high detection efficiency.

The present invention relates generally to constructs and their use in gene expression or gene regulation assays. More particularly, the present invention provides expression vectors and / or reporter vectors providing kinetics of protein expression with improved temporal correlation to promoter activity. Even more particularly, the invention provides expression vectors comprising a transcribable polynucleotide which comprises a sequence of nucleotides encoding a RNA element that modulates the stability of a transcript corresponding to the transcribable polynucleotide. The present invention provides, inter alia, novel vectors, useful for identifying and analysing cis- and trans-acting regulatory sequences / factors as well as vectors and genetically modified cell lines or organisms that are particularly useful for drug screening and drug discovery.

The invention discloses a multiplex fluorescence PCR (polymerase chain reaction) universal adapter for microsatellite detection, which comprises an adapter 1, an adapter 2, an adapter 3 and an adapter 4, wherein the nucleotide sequences of the adapters 1-4 are disclosed as SEQ ID:1-4. The universal adapter has the advantages of high PCR amplification efficiency and no interference, and can not interfere with microsatellite primers to be amplified. The invention also discloses a method for carrying out microsatellite multiplex fluorescence PCR detection by using the multiplex fluorescence PCR universal adapter for microsatellite detection. In the multiplex PCR process, primer screening and typing experiments can be performed only after the four or three fluorescence adapters are synthesized. The method has the advantages of simple steps, short experimental period and low experimental cost.

A process for replicating or for replicating and expressing a sequence of interest in a plant, comprising: (i) an RNA replicon or a precursor thereof, said RNA replicon being derived from a plus-sense single stranded RNA virus and comprising at least one sequence of interest; and (ii) a helper replicon, or a precursor thereof, wherein said helper replicon is (a) incapable of systemic movement in said plant both in the presence and in the absence of said RNA replicon (i) and (b) capable of expressing in a plant one or more proteins necessary for systemic movement of said RNA replicon (i), whereby said RNA replicon (i) is capable of replicating or replicating and expressing said sequence of interest in said plant, but unable to move systemically in said plant in the absence of said one or more proteins expressed by said helper replicon (ii).

The invention discloses a multiplex PCR (polymerase chain reaction) detection reagent of porcine circovirus type II, porcine parvovirus and porcine pseudorabies virus and special primers thereof. The invention provides the special primers for detecting the virus, and the special primers comprise a primer pair A, a primer pair B and a primer pair C, wherein the primer pair A comprises a primer 1 and a primer 2, the primer pair B comprises a primer 3 and a primer 4, the primer pair C comprises a primer 5 and a primer 6, and the nucleotide sequences of the primer 1, the primer 2, the primer 3, the primer 4, the primer 5 and the primer 6 are respectively as shown in the sequence 1, the sequence 2, the sequence 3, the sequence 4, the sequence 5 and the sequence 6 in a sequence table. Experiments prove that the multiplex PCR detection method of the porcine circovirus type II, the porcine parvovirus and the porcine pseudorabies virus provided by the invention is simple, convenient, fast, economical and efficient and has great application prospects in clinical diagnosis.

The present invention relates generally to constructs and their use in gene expression or gene regulation assays. More particularly, the present invention provides expression vectors and / or reporter vectors providing kinetics of protein expression with improved temporal correlation to promoter activity. Even more particularly, the invention provides expression vectors comprising a transcribable polynucleotide which comprises a sequence of nucleotides encoding a RNA element that modulates the stability of a transcript corresponding to the transcribable polynucleotide. The present invention provides, inter alia, novel vectors, useful for identifying and analysing cis- and trans-acting regulatory sequences / factors as well as vectors and genetically modified cell lines or organisms that are particularly useful for drug screening and drug discovery.

The invention discloses a ZIKV (zika virus) vaccine. The ZIKV vaccine contains or can express membrane protein prM / M, envelope protein E and non-structural protein NS1 of ZIKV. The invention also discloses a preparation method of the ZIKV vaccine. The ZIKV vaccine has higher immunogenicity and can more effectively prevent ZIKV infection.

The invention discloses a double-target spot embedded antigen receptor, an encoding gene thereof, a plasmid containing gene, an immune T effect cell containing the gene and application of the gene. The embedded antigen receptor contains a CD4 antigen region (including a Leader region), a Linker region, a 17b single chain antibody region (17bscFv), a hinge region, a membrane spanning structural domain, a co-stimulation structural domain and a signal conduction structural domain. A binding region of gp120 is simulated by virtue of CD4-17bscFv-CAR, and the validity and safety of CD4-17bscFv-CAR are far higher than the validity and safety of a previously reported single-target spot CD4-zetaCAR.

The invention belongs to the technical field of aquatic organisms and in particular relates to a quick detection method for scallop pathogenic vibrio splendidus. Total DNA (deoxyribonucleic acid) of bacterial genomes in a filtered seawater sample to be detected is taken as a template, fluorescent quantitative PCR (polymerase chain reaction) amplification is carried out by a bacterial 16SrDNA conserved region and a specific primer of a pathogenic vibrio splendidus metalloproteinases gene, and the scallop pathogenic vibrio splendidus in the culture environment is quantitatively analyzed and detected; the detection method comprises the steps of extracting the DNA of the bacterial genomes and carrying out fluorescent quantitative PCR detection. The method has the advantages that the quickness is achieved, the specificity is good, the bacterial density detection range is wide, and a quantitative result can be close to a real situation.

The invention discloses LAMP (loop-mediated isothermal amplification) primers, a kit and a method for detecting murine klebsiella pneumoniae. A visual detection method of murine klebsiella pneumonia which is suitable for clinical sample detection and has strong specificity and high sensitivity is established by designing the LAMP primers by virtue of a klebsiella pneumoniae tyrB gene sequence. Whether klebsiella pneumoniae exists or not can be detected quickly and accurately by observing whether green fluorescence exists or not, and the lowest detectable limit is 5.6 copy number / reaction. According to identification of a specific sequence region of a target sequence by four primers designed and screened, high specificity of LAMP diffusion is guaranteed, so that the LAMP primers are suitable for field application at basic level.

The invention discloses loop-mediated isothermal amplification (LAMP) primers, a kit and a method for detecting mouse Klebsiella pneumoniae. LAMP primers are designed by using the Klebsiella pneumoniae tyrB gene sequence to establish a high-specificity high-sensitivity Klebsiella pneumoniae visual detection method suitable for clinical sample detection. The green fluorescence is observed to quickly and accurately detect the existence of Klebsiella pneumoniae, wherein the lowest detection limit is 1.6 number of copies / reaction. The 6 designed and screened primers are used for identifying the specific sequence region of the target sequence, thereby ensuring the high specificity of LAMP amplification. The added loop primers greatly enhance the efficiency, have the advantage of short consumption time and are suitable for field application of the basic level.

The invention discloses a stress-resistant gene SiRLK35 of setaria italica L.Beauv. as well as encoding protein and an application. Receptor-like protein kinase SiRLK35 is separated from setaria italica L.Beauv., the nucleotide sequence of the receptor-like protein kinase SiRLK35is shown in SEQ ID NO.1, and the gene encoding protein product belongs to serine / threonine protein kinase. The expression pattern of the gene in a stress condition is researched, the prokaryotic expression vector of the gene is established, the gene can be expressed in the protein level as is verified, the functions of the gene are primarily verified with a dot assay through a gene prokaryotic expression system, meanwhile, the growth curve of the gene under a salt treatment condition is detected, the gene is heterologously transformed into rice, a corresponding transgenetic plant is obtained, the functions of the transgenetic plant in salt resistance and stress resistance are identified primarily, targeted quality and character improvement on setaria italica L.Beauv. is further performed purposefully, the new stress-resistant germplasm of setaria italica L.Beauv. is innovated, and a guidance function is performed.

The invention relates to a transgenic cotton detection chip, which comprises a substrate and a nucleic acid probe positioned on the substrate, wherein the nucleic acid probe may be one or more of nucleic acid probes having the sequences SEQ ID No.3, SEQ ID No.5, SEQ ID No.8, SEQ ID No.11, SEQ ID No.14, SEQ ID No.17, SEQ ID No.20 and SEQ ID No.23. The invention also relates to a kit having the transgenic cotton detection chip, a method for detecting transgenic cotton and the use of the transgenic cotton detection chip and the kit in the detection of the transgenic cotton. The transgenic cottondetection chip can be used to detect the transgenic cotton simply, quickly, specifically and sensitively with high throughput.

The present invention relates to immunogenic peptides, including variants and analogs derived from Streptococcus pneumoniae (S. pneumoniae) proteins, to peptide-multimers, conjugates and fusion proteins that include such peptides, and to vaccines that include such immunogenic entities. In particular, the present invention relates to the use of such vaccines for eliciting protective immunity to S. pneumoniae.

The present invention provides a Brevundimonas sp. strain SD212-derived peptide having β-ionone ring-2-hydroxylase activity and a gene encoding the same, to thereby make it possible to produce rare carotenoids in which a hydroxyl group is introduced at the position 2 (2′) carbon in their β-ionone ring in large quantities. The present invention also provides a novel gene encoding an enzyme which introduces a hydroxyl group at the position 3 (3′) carbon in the β-ionone ring of carotenoids, and a novel gene encoding a geranylgeranyl pyrophosphate synthase.

The present invention relates to compositions and methods for creation of vector nucleic acid sequences (e.g., retroviral nucleic acid sequences) that comprise two or more exogenous nucleic acid sequences that encode highly homologous (e.g., identical) polypeptide sequences, yet wherein at least one of the exogenous nucleic acid sequences has been mutated using degenerate codons for purpose of reducing homology between the two or more exogenous nucleic acid sequences while maintaining the encoded polypeptide sequence. Preferred nucleic acid sequences include those encoding multi-chimeric immune receptor (CIR) genes. Specific nucleic acid sequences of such CIR genes are also disclosed.

The present invention discloses a specific primer sequence of cattle Y-chromosome and its application in cattle embryo six identification. Cattle ear tissue DNA is first extracted to perform primer specificity identification; and embryo cell sample DNA is then extracted to perform PCR sex identification. The bull sample may be amplified to 255 bp segment when external primer is used in PCR reaction and to 205 bp when internal primer is used, while cow sample has no any amplified product, and this shows the bull specificity of the primer and the applicability of the primer in identifying cattle embryo sex. The present invnetion has the advantages of less pollution, high accuracy and high sensitivity and is suitable for field operation.

The invention relates to a novel antigen epitope peptide based on CD271 and application of the novel antigen epitope peptide. The antigen epitope peptide is: (1) located in SEQ ID NO.1; or (2) is located in an amino acid sequence having at least 80% identity with the SEQ ID NO.1 sequence in (1); wherein SEQ ID NO.1 contains one or more antigen epitopes, and the length of the amino acid sequence of the antigen epitope peptide is 5-70% of the full length of the SEQ ID NO.1. An antibody, a nucleic acid aptamer, a vaccine, nanoparticles and the like targeting the antigen peptide can be stably combined with natural cells with CD271 protein. The antigen epitope peptide or nucleic acid, fusion protein, carrier or host cell and the like containing the antigen epitope peptide have important application significance in the fields of preparation of CD271 related detection reagents, disease treatment biological drugs, vaccines and the like.

The invention discloses an chimeric antigen receptor gene for HIV-1. The gene comprises a guide subregion, a structural domain, a hinge region, a trans-membrane structural domain, a co-stimulation structural domain, a signal transduction structural domain and a suicide gene system region, wherein the structural domain is combined with a CD4 binding site of a gp120. The invention further disclosesa plasmid with the gene, a T cell, a kit and application. According to the chimeric antigen receptor gene for the HIV-1, the plasmid with the gene, the T cell, the kit and the application, when the Tcell is applied, HIV-1 infection can be prevented, and meanwhile, the lethality on an HIV-1 infected cell can be improved; and once an HIV-1-CAR-T cell is input, adverse reactions occur, the cell in abody can be cleared at once through a suicide gene system, and therefore the preventive effect can be achieved.

The invention discloses dual RT-PCR (reverse transcription-polymerase chain reaction) detection for a CDV (canine distemper virus) and a CPIV (canine parainfluenza virus) and a special primer for the detection. The invention provides the special primer for virus detection. The primer comprises a primer pair A and a primer pair B, wherein the primer pair A comprises a primer 1 and a primer 2; the primer pair B comprises a primer 3 and a primer 4; and nucleotide sequences of the primer 1, primer 2, primer 3 and the primer 4 are sequence 1, sequence 2, sequence 3 and sequence 4 respectively in a sequence table. Experiments prove that the special primer for dual RT-PCR detection for the CDV and the CPIV has good sensibility, can at least detect the RNA (ribonucleic acid) content to be about 1*10<-3>ng / mu L, the sensitivity is far higher than that of 1 ng / mu L. A dual RT-PCR detection method is simple, convenient, fast, economical and efficient, and has good application prospect.

The invention discloses salinimonas profundi 13199. The salinimonas profundi has the preservation number being CGMCC1.17396 and a classification name being Salinimonas profundi. The strain is a new bacterial species derived from deep sea, and contains a special CRISPR-Cas system and a large number of heavy metal resistance genes. The sequence homologies of the Cas protein and a protein which is included in a high-quality protein database UniProtKB / Switch-Prot subjected to a certain degree of functional researches are lower than 65%, and possibly have different activities and characteristics. The strain Salinimonas prodi 13199 and the CRISPR-Cas system thereof are novel materials for developing and utilizing the CRISPR-Cas system and performing related researches, and furthermore, the strain has a certain application prospect in the aspects of heavy metal polluted environmental modification preparations.

The present disclosure relates to a novel anti-HER2 antibody or an antigen-binding fragment thereof used in the prevention or treatment of cancer, a chimeric antigen receptor including the same, and uses thereof. The antibody of the present disclosure is an antibody that specifically binds to HER2 which is highly expressed in cancer cells (particularly, breast cancer or gastric cancer cells), and binds to an epitope that is different from an epitope to which trastuzumab binds.

The present invention relates to a novel anti-HER2 antibody or antigen-binding fragment thereof used in the prevention or treatment of cancer, a chimeric antigen receptor comprising the same, and usesthereof. The antibody of the present invention is an antibody that specifically binds to HER2 which is highly expressed in cancer cells (particularly, breast cancer or gastric cancer cells), and binds to an epitope that is different from an epitope to which conventional trastuzumab binds. Compared to transtuzumab, the antibody of the present invention exhibits a better killing ability with respect to kill HER2-unexpressed cancer cells which have non reactivity (or resistance) to a trastuzumab antibody or have reduced sensitivity. In addition, when the anti-HER2 antibody of the present invention is administered in combination with trastuzumab, a synergistic killing ability with respect to a cancer cell line on which the trastuzumab antibody acts. Therefore, a composition of the present invention can be very usefully used for combined administration with a trastuzumab antibody for the treatment of cancer, and for the treatment of cancer not treated by trastuzumab.

The invention discloses application of salmonella gallinarum SifA protein in preparation of an ELISA antibody detection kit for detecting a salmonella gallinarum antibody. According to the invention,the salmonella gallinarum SifA protein is expressed and purified as a coating antigen of ELISA; the working procedure of the kit is optimized; standard positive serum of salmonella pullorum, salmonella enteritidis, salmonella typhimurium, escherichia coli, pasteurella, bordetella and avibacterium paragallinarum is prepared from SPF chickens, standard negative serum is prepared from healthy SPF chickens, the kit is used for detecting the prepared positive serum and negative serum to prove that the kit has good specificity. The kit provided by the invention detects a large amount of clinical serum, and is compared with a glass plate agglutination test commonly used for clinically detecting salmonella to prove the practicability of the kit. The kit can be used for detecting antibodies after salmonella gallinarum infection, and a rapid and accurate antibody detection method is provided for salmonella gallinarum diseases.

The invention aims to provide nucleotide primers for detecting porcine parvovirus and porcine circovirus type 2, which respectively are PPVF, PPVR, PCV2F and PCV2R, wherein the sequences PPVF and PPVR are a sense primer and an antisense primer for detecting the porcine parvovirus; the sequences PCV2F and PCV2R are a sense primer and an antisense primer for detecting the porcine circovirus type 2. A porcine parvovirus and porcine circovirus type 2 double-PCR detection method provided by the invention can simultaneously detect two viruses of PPV and PCV2 by one-time reaction; two pairs of used primers have good specificity; homology and complementarity between the primers are low; the primers have good sensitivity. The method has the advantages of simplicity, convenience, rapidness, economy, high efficiency and the like, is beneficial for a great quantity of clinic detection and has a good application prospect in clinical diagnosis.

The invention discloses a high-temperature-resistant cabbage type rape glyoxalase gene and protein as well as applications thereof, and relates to the technical field of mutation and genetic engineering. The nucleotide sequence of the high-temperature-resistant cabbage type rape glyoxalase gene namely a BnGLYI gene is shown by SEQ ID NO1; and the amino acid sequence of the protein of the high-temperature-resistant cabbage type rape glyoxalase gene namely the BnGLYI gene is shown by SEQ ID NO2. The invention lays an experimental foundation for boosting the resource development of good genes of cabbage type rapes, and improving the heat resistance and cold resistance of the cabbage type rapes; heat-resistant and cold-resistant gene resources are further expanded; the BnGLYI gene is converted into yeast cells to ensure that the heat resistance and cold resistance of the yeast cells are significantly improved, so that the heat resistance and cold resistance of other microorganisms and plants are expected to be further improved.