Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Hiro

115results about How to "Free from infection" patented technology

Method for preparing biological bactericide for fruit trees by utilizing propolis and beewax

The invention provides a method for preparing a biological bactericide for fruit trees by utilizing propolis and beewax. The method comprises the following steps of (1) soaking original raw glue or covering cloth for two times with edible ethanol, merging the soak solutions, adding CH3COOH solution for precipitation to remove lead, and filtering, then carrying out concentration in a vacuum concentration tank, controlling the material temperature, interlayer water temperature and atmospheric pressure of the tank and braising extract-shaped propolis in the tank after concentration is completed to obtain refined propolis; (2) smashing the refined propolis into small pieces and adding alcohol to dissolve the small pieces of refined propolis to prepare propolis solution; (3) decontaminating, drying and grinding eugenia caryophyllata, amur cork tree bark and rhizoma polygonati, mixing the powder according to parts by weight, extracting the mixture with alcohol according to the volume ratio and filtering the solution to obtain traditional Chinese medicine solution; (4) firstly melting lard, secondly stirring beewax and liquid paraffin in a stirrer and controlling the temperature and the rotating speed, thirdly adding and stirring the molten lard and propolis solution and controlling the temperature, rotating speed and time and fourthly adding and stirring the traditional Chinese medicine solution, stopping heating after a certain time, cooling the mixture while stirring so as to obtain the biological bactericide after complete cooling. The biological bactericide has the function of preventing fungi diseases of fruit trees and has the effects of improving the fruit quality and increasing the fruit yield.
Owner:GANSU APICULTURE TECH PROMOTION STATION

Chinese medicinal herbal plant liquid cotton gauze sanitary towel

The invention relates to cotton gauze sanitary towel which is immersed in or sprayed with Chinese medicinal herbal plant liquid. The Chinese medicinal herbal plant liquid for immersing or spraying the sanitary towel is prepared from Chinese medicinal herbal raw materials such as nacre, polygonum paleaceum wall, garden burnet root, common ducksmeat herb, cyrtomium rhizome and the like and water according to a ratio; and the cotton gauze sanitary towel is immersed in or sprayed with the Chinese medicinal herbal plant liquid, and after liquid medicine is absorbed by the sanitary towel fully, the sanitary towel is dried and thus, the Chinese medicinal herbal plant liquid cotton gauze sanitary towel is obtained. The Chinese medicinal herbal plant liquid cotton gauze sanitary towel has the effects of resisting bacteria, disinfecting, killing insects, relieving itching, dissolving stasis, and relieving pain, clearing heat, detoxicating, activating meridians and promoting blood circulation, regulating qi and blood, smoothing menstrual period and the like, can balance the pH value of female vaginae effectively and inhibit bacterial breeding, and has the excellent treatment effect of abnormal menstruation, dysmenorrheal and pudendal pain, pruritus vulvae, swelling, pudendal hygro-erosion, peculiar smell and the like which are caused by multiple vulvar diseases. Except for the menstrual period, the Chinese medicinal herbal plant liquid cotton gauze sanitary towel also produces the obvious curative effect on various common gynecological diseases when worn at ordinary times, and achieves the effects of treatment with external application and positive prevention.
Owner:李明杰

Breeding method of specific pathogen free reed vole

InactiveCN101803583AFree from infectionAdvantages Purification effectSurgical veterinaryAnimal husbandryMicrotus fortisPurification methods
The invention relates to a breeding method of a specific pathogen free (SPF for short) reed vole, which comprises the following steps in sequence: (1) breeding a male reed vole and a female reed vole as a pair in each cage and making the female reed vole pregnant; (2) determining the laparotomy time of the pregnant reed vole through palpation and observation of the pregnant reed vole; (3) disinfecting to prepare for laparotomy and extraction; (4) carrying out laparotomy and extraction for the reed vole; (5) breeding the newly born reed vole with a SPF CR mice within 1-5 day(s) and breeding the newly born reed vole with a SPF SD rat within 6-15 days; and (6) carrying out microbiologic and parasite detection for items necessary to be detected in GB14922.1-2001 and GB14922.2-2001. The laparotomy and extraction are carried out under sterile conditions, and two types of breeding mouse completely different from the carcass supply mice are adopted for breeding and purification, thereby achieving the purification effect with more advantages than the medical purification method, successfully obtaining the SPF reed vole for the first time, and laying a foundation for the establishment of an ideal animal model for schistosomiasis resistance, non-alcoholic fatty liver and other diseases and the research on pathogenic mechanisms of the diseases.
Owner:上海西普尔-必凯实验动物有限公司

Biological safety enzyme-linked immunosorbent assay workstation and method for performing enzyme-linked immuno sorbent assays by utilizing same

The invention provides a biological safety enzyme-linked immunosorbent assay workstation and a method for performing enzyme-linked immuno sorbent assays by utilizing the same. The biological safety enzyme-linked immunosorbent assay workstation comprises a sample injecting device, a cleaning device, a warm bathing device, a colorimetric analyzing device and a biological safety protecting device. The sample injecting device comprises a three-dimensional-motion manipulator and an injection mechanism. The cleaning device comprises an injecting needle washing device, a plate cleaning device and cleaning buckets. The biological safety protecting device comprises a fan, an upper filtering mechanism and a lower filtering mechanism. The method for performing the enzyme-linked immuno sorbent assays by utilizing the biological safety enzyme-linked immunosorbent assay workstation includes that 1) reagent are sucked by the injecting needle; 2) samples or the reagent are distributed to cuvettes to perform reactions; 3) health statues of the assaying samples are determined by the colorimetric analyzing device according to absorbance values; 4) the cuvettes are cleaned by the plate cleaning device; 5) entire pipeline and the injecting needle is washed by cleaning fluid; 6) other samples or reagent are added; and 7) ultraviolet lamps are turned on to perform disinfection.
Owner:BIOBASE BIODUSTRY (SHANDONG) CO LTD

Pollution-free strawberry planting method

The invention relates to the field of strawberry planting and discloses a pollution-free strawberry planting method. The method comprises the following steps: (1) seedling culture: a seedling obtained through tissue culture and virus-free treatment is hardened and cultured, the seedling with good tolerance is screened layer by layer, and meanwhile, the infection of the seedling by viruses and pests is also avoided before transplanting, so that the survival rate is increased, and the infection rate of diseases and pests is reduced; (2)transplantation: the invasion of the strawberry seedling by diseases and pests hidden in the planting soil is mainly isolated, and meanwhile, a root protection material is used to prevent breeding of bacteria and pests on the strawberry leaf in a fertilizing process; and (3) maintenance: possible diseases of the strawberry are prevented and controlled by a plant disease and pest resistance preparation, and meanwhile, an artificial insect trap is used to solve the problems of fungal diseases and pests like red spiders and whiteflies bred quite easily in a greenhouse. The pollution-free strawberry planting method has the prominent advantages that no hormone pesticide is applied onto the strawberry, and the obtained strawberry is natural, pollution-free, large, solid, and high in sugar content.
Owner:蚌埠市乔峰农业蔬菜专业合作社

Preparation method of anti-neweastle disease virus specific transfer factor and oral liquid, and use thereof

The invention provides a preparation method of an anti-neweastle disease virus specific transfer factor. The method comprises the following steps: selecting a healthy chicken as an experiment chicken to carry out supplementary immunization of a newcastle disease inactivated vaccine; monitoring the level of an immune antibody of the newcastle disease vaccine inside the experiment chicken body; taking splenic organs of the experiment chicken in a sterile condition when the level of the antibody achieves 28-29; and processing the splenic organs of the chicken, so as to obtain the anti-neweastle disease virus specific transfer factor. By adopting the anti-neweastle disease virus specific transfer factor disclosed by the invention, the specific immune effect of the chicken body on a neweastle disease virus antigen can be improved; normal cells of the body can be prevented from being infected by the virus. Thus, the transfer factor disclosed by the invention can play the roles in preventing the neweastle disease virus infection and protecting normal cells of the body from being damaged by the neweastle disease virus, so as to reduce the morbidity; meanwhile, the chicken infected by the neweastle disease virus can be treated by using the transfer factor. Therefore, the clinical symptoms can be effectively improved; the recovery rate is increased; the mortality is reduced.
Owner:SHANDONG SINDER TECH

Brassica chinensis cultivation method with using wormcast produced from waste of sugar refinery

The invention provides a brassica chinensis cultivation method with using wormcast produced from waste of a sugar refinery, and belongs to the technical field of vegetable plantation. The method comprises the steps that (1) a cultivation matrix is prepared by mixing bagasse, sugarcane filter mud, coal ash, wine slag, a calcium magnesium phosphate fertilizer, peanut bran and a fermented strain uniformly and stacking the same for fermentation, and adding an active nutrition agent, including cytex and thiamine hydrochloride, to wormcast 50-60 days after introduction of earthworm to obtain a cultivation matrix; (2) seeds are soaked and germination is accelerated; (3) sowing and seedling growing are carried out, plantation soil is sprayed with a weak trichoderma harzianum solution after sowing;and (4) thinning and field management is carried out by a foliar fertilizer is applied once in a six-leaf-one-center period of the brassica chinensis. According to the method, waste of the sugar refinery is changed into valuables, the waste is fermented and used for earthworm culturing, the wormcast is used for cultivation of brassica chinensis, the application amount of inorganic fertilizers forbrassica chinensis can be reduced, and the output and taste of brassica chinensis are ensured.
Owner:GUANGXI UNIV

Influenza new coronary combined vaccine and preparation method thereof

The invention discloses an influenza new coronary combined vaccine. The influenza new coronary combined vaccine is prepared from the following raw materials in mass concentration: 1 to 100 micrograms/mL of recombinant new coronary vaccine containing RBD-Fc fusion protein, 1 to 50 micrograms/mL of influenza subunit vaccine containing H1N1 type influenza virus, 1 to 50 micrograms/mL of influenza subunit vaccine containing H3N2 type influenza virus, 1 to 50 micrograms/mL of influenza subunit vaccine containing B type influenza virus, an aluminum hydroxide solution and the balance of PBS phosphate buffer, wherein the final concentration of aluminum ions in the influenza new coronary combined vaccine is 0.5 to 2.0mg/mL. A preparation method comprises the following steps of: weighing all the raw materials; diluting the four vaccines with the PBS phosphate buffer respectively and then mixing with the aluminum hydroxide solution; and mixing according to an equal volume ratio to obtain the influenza new coronary combined vaccine. The influenza new coronary combined vaccine disclosed by the invention is a combined vaccine of a novel coronavirus vaccine containing an aluminum adjuvant and the influenza subunit vaccine containing the aluminum adjuvant; and after combination, the two antigen component vaccines are not mutually inhibited and can be very well compatible.
Owner:天津中逸安健生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products