The invention discloses formula powder suitable for females at the pregnancy period and a preparation method of the formula powder, and belongs to the technical field of concoction milk powder and a nutrient food. The formula powder consists of lactoferrin, casein peptide phosphate, folic acid, ferrous gluconate, whey protein powder, galactooligosaccharide, fructo-oligosaccharide, docosahexaenoicacid, choline, taurine, vitamin A, vitamin D, vitamin C, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacin, biotin, calcium, magnesium, phosphorus, sodium, potassium, zinc,selenium and fresh milk spray-dried powder. The formula powder is suitable for females at the pregnancy period, various nutrient substances can well promote absorption with each other and provide nutrients necessary for females at the pregnancy period without additional nutrient enrichment, and the formula powder can enrich the calcium, can resist anemia, can improve the immune function and disease resistance of the females at the pregnancy period, can make early-stage preparation for the females at the pregnancy period, and finally, can realize the purpose of prenatal and postnatal care.