59results about How to "Improved germplasm" patented technology

The invention relates to a method for ecologically interplanting sarcandra glabra under a phyllostachys edulis forest. The method comprises the steps of: (1) tissue culture and seedling raising: sarcandra glabra blades are selected as explants to conduct adventitious bud induction, an adventitious bud induction culture medium uses an MS (Murashige and Skoog) culture medium as a basic culture medium and additionally comprises 2.0 to 4.0mg/L of TDZ (thidiazuron), 0.1 to 0.2mg/L of NAA (naphthyl acetic acid), 100 to 150g/L of banana puree and 0.5 to 1.0g/L of activated carbon, and then adventitious buds are subjected to enrichment culture and rooting culture; (2) seedling hardening: bottom seedlings are conveyed to under the phyllostachys edulis forest to be hardened; (3) transplantation: tissue culture seedlings are transplanted to a north slope bamboo forest with a number of living stems (NLS) of phyllostachys edulis of 150 to 400 plants/mu or a south slope bamboo forest with an NLS of 200 to 400 plants/mu; (4) forest management: the second intertillage is conducted in June and September every year; and (5) harvest: the sarcandra glabra is harvested in November to December every year. By adopting the method, the culture period of the sarcandra glabra can be shortened and the yield of the sarcandra glabra can be increased.

The invention discloses a method for selecting and breeding improved variety types of walnuts and belongs to the technical field of agriculture and biology. The method comprises steps of rootstock seedling selecting and breeding, scion collecting and processing, grafting, transplanting to land. The scions are selected from superior mother trees in native soil. The improved variety types of walnuts selected and bred after inlay-bark-tongue grafting has following advantages: good yield ability, large size, good appearance and early ripeness. The walnuts grow well especially in dry conditions of hilly areas, show properties of good drought tolerance, cold tolerance and disease resistance, and are suitable for promoting and planting in similar area. Green fruits of the improved variety types of walnuts are suitable for sale. The thickness of shells is suitable for machining. The walnuts are storable and transportable. The method promotes the development of walnut industry and increases the improved variety degree of walnut production.

The invention discloses a rapid propagation method of a vesicularia protonema and gametophyte. The method includes disinfecting vesicularia sporule; preparing a spore suspension; inoculating the sporesuspension to an LQ culture medium for culture; germinating the spores to generate the protonema; subculturing and expanding the protonema to obtain a large number of new gametophytes; grinding and crushing the vesicularia protonema or/and gametophytes by using a homogenizer; and inoculating the crushed moss suspension to the LQ culture medium for culture. The method establishes a vesicularia tissue culture system for the first time, can obtain a large number of the vesicularia protonemata and gametophytes in a short time, provides a large amount of experimental materials and technical support for genetic improvement of vesicularia, and further provides rich provenances for gardening, landscaping and the like by applying the vesicularia.

The invention discloses an artificial breeding method for loaches. The artificial breeding method is characterized by including the following steps that A, the disease and injury-free loaches with the regular body shape, normal body color, more body surface slime and winter age being 2-4 years are selected, the selected parent loaches are screened through a stainless steel round hole screen with the diameter being 1.5-2cm, and individuals with the small body shape are removed; B, anesthetic is thrown into a storage pond with the water temperature being 20-25 DEG C, and after the parent loaches are anesthetized, the parent loaches are subjected to injection spawning induction with odinagogue; C, fertilized eggs are placed into a debonding device after being fertilized, and debonding is performed on the fertilized eggs in stirring and water flow modes under the actions of the constant temperature of 24-25 DEG C and circulating water; D, after the loaches achieve artificial fertilization and debonding, the fertilized eggs are evenly splashed into a cement pit for still water incubation; E, single-celled algae and rotifers are cultivated in the same cement pit of the step D, so that weaning food is provided for spraying seedlings of the loaches; and F, after the loach seedlings are fed for 10 days in a nursery pond, the loaches can be sold or placed into an earth pond for raising when reaching the inch seedling specification. The method is mainly used for artificial breeding of the loaches.

Provided is a method for screening a phosphorous high efficiency utilizing variety from multiple barley varieties. The method comprises the steps of selecting barley varieties with the same sizes of grains, putting the varieties into a culture dish, and germinating the varieties into seedlings with roots; selecting seedlings of which growth is consistent from the seedlings, dividing the seedling of which growth is consistent into two groups, and using a nutrient solution with different phosphorus concentration to pour on the seedlings until the barley is mature; measuring and calculating the relative plant height, the relative kernels per spike and the relative thousand seed weight of each variety, conducting comprehensive comparison, and selecting several barley varieties of which the three indexes are comprehensively the highest as phosphorus high efficiency utilizing varieties; germinating the barley varieties into after-screening seedlings with roots, selecting the after-screening seedlings of which the growth is consistent, dividing the after-screening seedlings into two groups, using the nutrient solution with different phosphorus concentration to conduct water planting, and measuring and calculating the relative dry matter weight, the relative phosphorus content and the relative chlorophyll content of each variety; comprehensively comparing, and selecting one barley variety of which the three indexes are comprehensively the highest as the variety of which the phosphorus utilizing efficiency is relatively the highest. According to the method for screening the phosphorous high efficiency utilizing variety from multiple barley varieties, the finally screened barley variety provides materials for further improving an existing variety and studying the biological characteristics of the existing variety and a physiology and biochemistry mechanism.

The invention relates to a method for breeding high-quality wheat. The method comprises the following steps of: hybridizing by selecting Zhengmai 005 as a female parent and Aizao 781 as a male parent, screening later generations of the Zhengmai 005 and the Aizao 781 to obtain Xufeng 998, and hybridizing by selecting Meisheng 0308 as a female parent and the Xufeng 998 as a male parent to obtain F1generation; back-crossing the F1 generation of plants and the wheat material, namely the Xufeng 998, and screening plants which are short in stalk, premature, more in tiller and good in disease resistance from one half of sterile plants; carrying out back-crossing for many times of the screened sterile plants and the wheat material, namely the Xufeng 998, and obtaining the screened wheat, namely the high-quality wheat. The bred high-quality wheat disclosed by the invention has the advantages of being hard in grain cutin, short in stalk, premature, more in tiller and good in disease resistanceand stress resistance.

The invention provides a detoxification method for cultivating anthers of Perfume Lily. The detailed steps of the detoxification method include: (1) preparing an anther induction medium for Perfume Lily; (2) selecting materials for inoculating anthers; (3) disinfecting and inoculating anthers; (4) Anther induction culture; (5) Embryogenic callus differentiation and subculture; (6) Hardening and transplanting of lily bulbs; (7) Elimination of haploid plants of lily. The anther culture and detoxification method of perfume lily of the present invention obtains perfume lily pollen plants through the cultivation of perfume lily anthers, and then removes the haploid plants doped in the population to obtain perfume lily diploid plants, and further detects the lily virus and finds that the The detoxification method almost achieves 100% detoxification, greatly improves the lily germplasm, and obtains detoxified lily plants with greatly improved yield and quality.

The invention provides an anther culture detoxification method of edible lily. The anther culture detoxification method includes: (1), preparing an edible lily anther inducing culture medium; (2), selecting materials for inoculating anther; (3), disinfecting and inoculating the anther; (4), performing inducing culture on the anther; (5), performing embryonic callus differentiation and subculture; (6), hardening and transplanting edible lily bulbs; (7), removing edible lily haploid plants. Edible lily pollen plants are obtained through an anther culture path, and haploid abnormal plants in an anther cultural population are removed to obtain edible lily diploid plants; further lily virus detection of detoxification plants finds that the detoxification method almost realizes 100% detoxification, production experiments further find that both yield and quality of edible lily detoxification plants are improved greatly.

The invention discloses a method for breeding a novel waterlogging-tolerant maize variety by using the special waterlogging tolerance of the teosinte. By transferring the excellent waterlogging tolerance of the teosinte to the maize inbred line so that the maize inbred line has excellent waterlogging tolerance, the novel maize inbred line with excellent waterlogging tolerance can be obtained rapidly, the breeding time can be shortened, the excellent character of the germ plasm resource of the teosinte can be transferred to the maize, the germ plasm of the maize can be extended, the waterlogging-tolerant genes of the maize can be found, and the way of making the maize to have waterlogging tolerance can be disclosed.

The invention discloses an ecological totally-enclosed circulating mariculture system which comprises a fish culture pond, a purification pond and a circulating unit. The fish culture pond has an area of 25-30 m<2> and water depth of 1.0-1.5 m; red sea bream is cultured in the fish culture pond; the putting density of the red sea bream is 10-20/m <3>; and the body length of the red sea bream is 12.0 +/-3.0 cm, and the weight of the red sea bream is 56.4 +/-25.0 g. Sesuvium portulacastrum is cultured in the purification pond, the specification of the purification pond is (3.5-4.5)m * (5.5-6.5)m, the water depth of the purification pond is 0.5-1.0 m, the purification pond is divided into 3-4 water channels at equal intervals, the water channels are connected in series end to end, and water flow bypasses the water channels in an S shape; and the density of the sesuvium portulacastrum is 20-30 plants/m<2>, the sesuvium portulacastrum plants are 35.9 +/-15.0 cm, and the average wet weight is 212.6 +/-100.0 g/plant. The circulating unit is used for enabling water in the fish culture pond to flow into the purification pond and enabling water in the purification pond to flow into the fish culture pond; and the culture water is circulated for 5-10 times every day between the fish culture pond and the purification pond. A breeding-planting-water quality purification three-in-one ecological culture system is constructed, and a benign composite ecological circulation system which can recycle energy and is stable in structure is formed.

The invention relates to the technical field of aquaculture, in particular to a treatment method for parent soft-shelled turtles before stocking. The treatment method is characterized by comprising the following steps of a, source and selection of the parent soft-shelled turtles: firstly, source of the parent soft-shelled turtles: soft-shelled turtles reaching sexual maturity, which are wild or obtained by artificial breeding and are bred by non-consanguineous mating; secondly, selection of the parent soft-shelled turtles: requiring the morphology of the parent soft-shelled turtles to accord with classification features of Chinese softshell soft-shelled turtles, namely complete shape without disability or distortion, normal body color, bright skin, plump and elastic rim, weight of 1 to 3kg and strong constitution, wherein the requirements on female soft-shelled turtles are that the tails are short and cannot naturally extend out of the rims, the bodies are thick, and the distance between rear legs is wide. and the requirements on male soft-shelled turtles are that the tails are long and thick and strong, and can naturally extend of the rims, the bodies are thin, and the distance between rear legs is narrow; b, bathing treatment of the turtle bodies: bathing the turtle bodies in liquid medicine for 15 minutes. According to the treatment method disclosed by the invention, the disease resistance and the immunocompetence of the soft-shelled turtles can be enhanced, so that the reproductive survival rate and the reproductive output of the soft-shelled turtles can be increased.

The invention relates to a method for creating a rehmannia heterozygous mutant and application of the rehmannia heterozygous mutant, and belongs to the technical field of molecular biology. A CRISPR/Cas9 system targets a rehmannia RgPDS1 gene, a nucleotide sequence of a coding region of the RgPDS1 gene is as shown in SEQ ID NO.1, and a target site sequence is as shown in SEQ ID NO.2. A pair of complementary upstream and downstream primers is synthesized according to the target sequence; after the primers are annealed to form double strands, the double strands are inserted into a CRISPR/Cas9 plant expression vector pKSE401 through an enzyme digestion connection method, and successfully constructed recombinant plasmids are further screened out; and the recombinant plasmid is transformed into agrobacterium tumefaciens, and a rehmannia explant is infected by adopting a leaf disc method genetic transformation technology to obtain the heterozygous mutant plant. The CRISPR/Cas9 gene editing system is used for creating the heterozygous mutant in the rehmannia for the first time, a new research means is provided for accelerating the rehmannia molecular breeding process, improving varieties and innovating germplasm resources, and the functions of researching some key regulatory genes can be further achieved.

The invention discloses an SSR (Simple Sequence Repeats) primer composition and method for identifying high-quality germplasm of picria fe-tearrae Lour., is used for screening and identifying the high-quality germplasm of the picria fe-tearrae Lour., and belongs to the field of molecular identification of new varieties of plants. According to the invention, 9 pairs of core SSR primers are designed, and by utilizing the 9 pairs of core SSR primers, a feature SSR molecular marker identifying method of the high-quality germplasm of the picria fe-tearrae Lour. is successfully constructed, so thatthe high-quality germplasm of the picria fe-tearrae Lour. can be rapidly and accurately identified. According to the invention, based on the 9 pairs of SSR primers, a PCR (Polymerase Chain Reaction) amplified reaction is performed; and a capillary electrophoresis fluorescence detection method is adopted, so that the high-quality germplasm of the picria fe-tearrae Lour. can be directly judged according to the presence and size of an amplified product fragment. The SSR primer composition and method disclosed by the invention can be directly applied to molecular identification of the high-qualitygermplasm of the picria fe-tearrae Lour., and has good practicability.

The invention provides a breeding method for improving the commercial specification and the commercial rate of freshwater shrimps. The breeding method comprises the following steps: clearing a pond, and planting cold-resistant waterweeds; putting young shrimps bred in winter and spring; catching the freshwater shrimps for marketing for the first time; catching the freshwater shrimps for marketing for the second time; clearing the pond, and planting high-temperature-resistant waterweeds; putting young shrimps bred in summer; replacing bottom-layer water of the pond, and spreading quicklime, a bottom-sediment improver and an organic acid detoxifying agent into the pond; when the content of dissolved oxygen of the pond is 3 mg/L or less, introducing oxygen into the pond; and alternately applying the bottom-sediment improver and the organic acid detoxifying agent, and catching all the freshwater shrimps in the pond for marketing. The breeding method has the following beneficial technical effects: through technological innovation in the aspects of control of water quality, a stocking manner of the young shrimps, planting of the waterweeds, feed selection and feeding, catching, etc., a proper shrimp load density of the pond is maintained, and the survival rate of the freshwater shrimps is improved. Through technical measures of the invention, a unit net profit reaches 5000 to 8000 yuan which is 2 to 4 times of the unit net profit of traditional breeding.

The invention discloses a rapid propagation method adopting tissue culture for Croton tiglium. The rapid propagation method comprises steps of disinfection of Croton tiglium seeds, acquisition of aseptic seedlings; proliferation of cluster buds, rooting culture, strong seedling culture, acclimatization and transplanting. According to the method, a Croton tiglium regeneration system is established and can be applied to storage and reproduction of germplasm resources of the Croton tiglium as well as research on genetic improvement, and defects of a traditional mode for improving Croton tiglium germplasm can be overcome.

The invention relates to a germplasm improvement method for traditional Chinese medicinal materials. The germplasm improvement method comprises the steps of A germplasm selection, B germplasm improvement, C improved germplasm identification and screening, D improved germplasm rooting, E rooted seedling domestication and F improved germplasm seed obtaining. According to the method, colchicine is used for conducting polyploid induction on ainsliaea fragrans sterile adventitious buds, then screening and purification culture are conducted through a morphological identification method and a chromosome tabletting method, the improved ainsliaea fragrans germplasm is obtained, and the obtaining rate is 19%; according to the obtained improved ainsliaea fragrans germplasm, stems are obviously thickened, leaves are obviously thickened, the single plant biomass is 1.9 times higher than that of an original female parent, the yield per mu is 1.6 times higher than that of the original female parent, meanwhile, the chlorogenic acid content is 1.3 times higher than that of the female parent, and the protocatechuic acid content is 2.2 times higher than that of the original female parent; and the obtained high-quality germplasm has genetic stability, and plants planted through the seeds still have fertility.

The embodiment of the invention discloses a creation method for good germplasm of strawberries with red flowers and white fruits and belongs to the technical field of fruit tree breeding methods. Themethod comprises the following steps: selecting any variety of strawberry varieties "Little Peach Blossom", "Pink Panda", "Pink Beauty" and "Pretty Beauty" as a female parent, selecting any variety ofstrawberry varieties "Snow White", "Snow Rabbit", "First Love", "Pineapple Berry" and "Angel No.8" as a male parent, and carrying out hybridization. The strawberries obtained by the embodiment of theinvention have the pink flowers and the white fruits, seeds on the fruits are red, the strawberry germplasm with the red flowers and the white fruits is created for the first time, and the method issimple and safe, enhances ornamental values and also has high edibleness values.

The invention discloses a high-yield mussel breeding method based on fresh water pearls and belongs to the field of mussel seed breeding. According to the high-yield mussel breeding method based on fresh water pearls, for selection and breeding of a first generation of mussel parents, male and female mussels of optimal ages are selected as male and female parents, the number of the male and female parents is 300 pairs or more, the shells of the selected male and female parents are respectively marked, a natural fertilization group and an artificial fertilization group are provided, and multiple generations of mussels of the two groups are selected, fertilized and bred constantly, so that various excellent germplasms of offspring mussels are effectively improved in the multi-generation screening and breeding, meanwhile, larger pearls are easier to generate and the mellow and full degree of the pearls is improved through pearl-culturing mussels obtained through artificial pearl implanting inoculation, and the pearl secretion capacity of hyriopsis cumingii is effectively enhanced. After multiple generations of breeding of two groups of experiments, the high-yield varieties of freshwater pearls are effectively cultured through comparative yield analysis of the freshwater pearls.