59results about How to "Improved germplasm" patented technology

The invention discloses a method for obtaining high-polysaccharide and high-yield dendrobium officinale kimura ex migo through ion beam mutagenesis and application. The method comprises the followingsteps: selecting full and insect-pest-free seeds and disinfecting; carrying out ion beam mutagenesis treatment on the disinfected dendrobium officinale kimura ex migo seeds; carrying out tissue culture seedling cultivation on the mutagenic seeds; cultivating the obtained tissue culture seedling, selecting plants obtained by seeds which are not subjected to the mutagenesis treatment as a contrast,and screening to obtain high-yield plants with character mutation; independently collecting seeds of the plants with the character mutation and continually carrying out subculture; screening to obtainthe dendrobium officinale kimura ex migo with stable characters, so as to obtain a product. According to the method for obtaining the high-polysaccharide and high-yield dendrobium officinale kimura ex migo through the ion beam mutagenesis, provided by the invention, non-directional mutation can be generated after an ion beam is injected to carry out the mutagenesis; in a lot of mutagenesis screening experiments, screening can be carried out according to a pre-set screening target, so that excellent dendrobium officinale kimura ex migo germplasm with needed characters is screened an obtained;the polysaccharide content of the bred dendrobium officinale kimura ex migo is improved by 13.35 percent on the basis of improving the yield.

The invention belongs to the technical field of poultry breeding, and specifically relates to an auxiliary selection marker for goose belly fat weight and carcass weight for liver, which has a SNP site, and the SNP site is located at the 807th bp downstream of the start codon of the MC5R gene. The invention also discloses a method for using molecular markers to assist the selection of goose belly fat weight and carcass weight traits for liver use: select the Lande goose individual to be tested, draw blood and extract DNA, and use the provided kit to perform polymerase chain reaction (PCR) and detect the product, send the PCR product to the sequencing company for sequencing and determine the genotype, and select qualified individuals according to the genotype for breeding. The invention can not only avoid the high cost and heavy workload caused by the slaughter measurement method, and the disadvantages that excellent individuals cannot be reserved for breeding, but also can be used for large-scale selection and speed up the breeding process.

The invention provides a day lily germplasm detoxification and rejuvenation method. The day lily germplasm detoxification and rejuvenation method comprises the following detail steps: (1) selecting to-be-inoculated flower buds of day lily; (2) preparing a day lily anther induction medium; (3) disinfecting and inoculating anthers; (4) carrying out anther induction culture; (5) carrying out anther-derived callus differentiation and rooting culture; (6) carrying out hardening-seedling and transplanting of day lily seedlings; (7) removing non-diploid day lily plants. According to the day lily germplasm detoxification and rejuvenation method provided by the invention, day lily pollen plants are obtained through a day lily anther culture way, so as to further obtain day lily diploid plants by removing non-diploid day lily abnormal plants from the anther cultural population, the germplasm detoxification and rejuvenation method is significant in detoxification effect, the day lily germplasm can be rejuvenated, and the quality and yield of day lily are greatly improved.

The invention discloses a method for improving the lateral branch rooting survival rate and the seed reproduction quality of radish. The method comprises the following steps: a second-stage lateral branch is taken as a cutting branch, after the cut-off lateral branch heals naturally, the cut-off lateral branch is soaked in an indolebutyric acid solution for 10 seconds and then inserted in a seedbed substrate composed of coco coir, vermiculite and mushroom dreg; a mixed solution containing Huabao No.1, Huabao No.2 and naphthylacetic acid is sprayed on the branch so that the branch can be acclimated and then survives; when new leaves come out, transplanting is performed by use of an underground simple culture tank filled with planting soil which is composed of coco coir, vermiculite, mushroom dreg and decomposed cow dung; after the cutting lateral branch survives, ventilation and light illumination are realized, Huabao No.1 and Huabao No.2 are sprayed, and after seeds are mature, the seeds are harvested. The method for improving the lateral branch rooting survival rate and the seed reproduction quality of the radish has the advantages that the method of rooting by use of the second-stage cutting lateral branch of the radish is adopted, a new method is provided for solving the problem of seed reproduction material loss of the radish due to the infringement of soft rot, the plants cultured by use of the method are well developed in root system, high in transplanting survival rate, and stable in heritability, and furthermore, the seed reproduction can be saved.