The invention discloses a method for accurately identifying
spathiphyllum hylobatidaes by combining
environmental DNA and a
Taqman probe method, and belongs to the technical field of
biology.The method comprises the following steps that S1, eDNA to be detected is obtained, and real-time fluorescent quantitative PCR amplification reaction is conducted through
spathiphyllum hylobatidaes primers and probes; wherein the sequence of the primer H.lar-F of the
spathiphyllum hylobatidaes is as shown in SEQ ID NO.1, the sequence of the primer H.lar-R of the spathiphyllum hylobatidaes is as shown in SEQ ID NO.2, and the sequence of the probe H.lar-P of the spathiphyllum hylobatidaes is as shown in SEQ ID NO.3; and S2, if the detected CT value is less than 40, indicating that the to-be-detected eDNA contains the
DNA of the spathiphyllum hylobatidaes. According to the invention, the
TaqMan-MGB fluorescent probe with certain specificity and sensitivity is screened out, so that the probe can specifically detect the CT value of a
target gene segment, thereby realizing
rapid detection and identification on whether eDNA contains the
genome DNA of the spathiphyllum hylobatidaes or not, and judging whether the spathiphyllum hylobatidaes inhabit in the environment or not.