Spathiphyllum somatic embryo inducing and plant regenerating method

A technology for somatic embryos and plant regeneration, which is applied in the fields of botanical equipment and methods, plant regeneration, and horticultural methods. The time limit is relatively large and other problems, so as to achieve the effect of improved induction efficiency, easy acquisition, and convenient material acquisition.

Inactive Publication Date: 2013-05-01
广东省农业科学院花卉研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to reports, the efficiency of inducing somatic embryos is relatively low. Generally, only a single or a dozen somatic embryos can be produced on each explant; the collection of explants also needs to be in the flowering season, which is limited by the time limit of plant growth and development.
[0005] At present, there is no report on the successful induction of somatic embryos and plant regeneration from explants of vegetative organs such as leaves or petioles

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: the present embodiment is carried out through the following steps:

[0019] (1) Somatic embryo induction. Heliconia S. cannifolium The stem segments or terminal buds were disinfected and inoculated into clustered bud induction medium to obtain sterile test-tube plantlets, and then transferred to test-tube plantlet proliferation medium at a temperature of 24-30 °C and a light intensity of 1000-1400 lux, 10-14 hours of light per day, subculture once every 3-4 weeks, and the test-tube plantlets continue to proliferate. The leaves of young test-tube plantlets were cut into 1-10 mm wide strips, inoculated in somatic embryo induction medium, and cultured in a dark environment at a temperature of 24-30 °C for 2-4 weeks to obtain somatic embryos;

[0020] (2) Proliferation of somatic embryos: The obtained somatic embryos were subcultured in the somatic embryo induction medium, and cultured in a dark environment at a temperature of 24-30 °C for 2-4 weeks, the so...

Embodiment 2

[0024] Embodiment 2: The difference between this embodiment and Example 1 is that in step (1), the petiole of the test-tube plantlet is cut into 1-10 mm sections, and then inoculated in somatic embryo induction medium to obtain somatic embryos; the rest are the same as Example 1 is the same.

Embodiment 3

[0025] Example 3: The difference between this example and Example 1 or 2 is that the somatic embryo induction medium in step (1) uses MS medium as the basic medium, and each liter of medium contains 0.05-0.25 mg of thiophene Benuron, 0.5-2.0 mg of 2,4-dichlorophenoxyacetic acid, 20-40 grams of sucrose and 6-9 grams of agar, with a pH value of 5.6-6.0; somatic embryo proliferation medium in step (2) MS medium is used as the basic medium, and each 1 liter of medium contains 0.05-0.25 mg of thidiazuron, 0.5-1.0 mg of 2,4-dichlorophenoxyacetic acid, 20-40 grams of sucrose and 6~ The agar of 9 grams, pH value is 5.6~6.0; All the other are identical with embodiment 1 or 2.

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Abstract

The invention provides a spathiphyllum somatic embryo inducing and plant regenerating method, which relates to a somatic embryo inducing and plant regenerating method and comprises the following steps of: 1, taking young and tender spathiphyllum test tube seedlings, cutting blades or petioles into small sections to be induced, and obtaining somatic embryos; 2, subculturing the somatic embryos into a somatic embryo multiplication culture medium for mass multiplication; and 3, transferring the somatic embryos onto the plant regeneration culture medium for culture to obtain complete small plants. The method has the advantages that the somatic embryo inducing rate reaches 97 percent to 100 percent, 50 to 200 somatic embryos can be formed on the surface of an explant in the primary culture, the inducing efficiency is obviously improved, and the plant regeneration rate of the obtained somatic embryos reaches 85 to 95 percent. The method solves the problems that the existing spathiphyllum somatic embryo has low inducing efficiency, and the explant material collection is inconvenient.

Description

technical field [0001] The invention relates to the technical field of plant induced regeneration, in particular to a method for inducing and regenerating plants from somatic embryos of Heliconia chinensis. Background technique [0002] White crane taro ( Spathiphyllum Schott), also known as white palm or smooth sailing, is an important tropical ornamental plant native to Central and South America, and is very popular in European and American markets. Introduced to mainland my country in the 1980s, it rapidly developed into an industry and became one of the most important tropical ornamental plants in my country. [0003] The plant tissue culture of Heliconia chinensis has been reported since the 1970s. It mainly uses explants such as terminal buds and stem segments to induce clustered buds, expands the number of reproduction by clustering buds, and then obtains complete plants by rooting culture. Its purpose is to achieve in vitro rapid propagation, expand the number of i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 于波刘金梅刘晓荣廖飞雄
Owner 广东省农业科学院花卉研究所
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