67 results about "Cell division" patented technology

The invention relates to the field of molecular cytogenetics and potato ploidy breeding, in particular to a potato ploidy identification method for marking and counting chromosome centromere based on a potato CENH3 antibody and a ploidy detection kit. According to the method, the chromosome centromere is marked by specifically recognizing the antibody of the potato centromere nucleosome core histone CENH3 and combining the immunofluorescence technology, so that the accurate counting of the chromosome centromere is realized at the single cell level, and the ploidy of potatoes is judged. The method comprises the following steps: preparing a leaf cell suspension, sequentially incubating a potato CENH3 antibody and a labeled secondary antibody after immobilization, and observing and counting by a microscope after redyeing. The method has the advantages of high species specificity, high throughput and low cost, is not limited by special material taking parts of root tips or shoot tips and cell division periods, can accurately identify the uploid and aneuploid of potatoes, and provides an efficient tool for potato germplasm resource evaluation and cross breeding.

The invention belongs to the technical field of biological agents, and particularly relates to ginsenoside variant flavobacterium DG-16 and application thereof in relieving continuous cropping obstacles of angelica sinensis and improving the quality of the angelica sinensis. According to the ginsenoside variant flavobacterium DG-16 disclosed by the invention, the growth of angelica sinensis is promoted by increasing the contents of jasmonic acid and cytokinin hormones, reducing the contents of gibberellin and salicylic acid hormones and promoting the circulation and utilization of nitrogen; in addition, the flavobacterium ginsenoside variant DG-16 can significantly increase the chlorophyll content, is beneficial to photosynthesis of the angelica sinensis, effectively changes the physiological and biochemical states of the angelica sinensis, can resist root rot of the angelica sinensis so as to relieve successive cropping obstacles and improve the quality, and has a significant effect.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to rhizobium capable of promoting plant cell division, resisting salt and alkali and reducing alkali and application of the rhizobium. The strain number of the Neorhizobium alkalisoli is YRD01, the preservation number of the Neorhizobium alkalisoli is CCTCC NO: M 20251990, and the Neorhizobium alkalisoli is preserved in China Center for Type Culture Collection, Luojia Hill, Wuchang District, Wuhan City, Hubei Province on September 9, 2025. The strain obtained by the invention is derived from sesbania root nodules and is obtained by screening and culturing the sesbania root nodules. The rhizobium (Neorhizium alkalisol) YRD01 obtained by screening in the invention is a strain of novel rhizobium which has the characteristics of high saline-alkaline tolerance, active alkali reduction function and efficient growth promotion.

The invention relates to a recombinant vector, genetic engineering rhizobium and application of the genetic engineering rhizobium to improvement of heat resistance of leguminous plants. The recombinant vector contains a thermal induction promoter PkatC and a cytokinin synthetic gene ipt, the nucleotide sequence of the thermal induction promoter PkatC is shown as SEQ ID NO.1, and the nucleotide sequence of the cytokinin synthetic gene ipt is shown as SEQ ID NO.2. The invention further discloses a preparation method of the recombinant vector. According to the method, a recombinant vector pSRK-PkatC-ipt containing a thermal induction promoter PkatC and a cytokinin synthetic gene ipt is transferred into sinorhizobium meliloti, genetic engineering rhizobium is constructed and obtained, and the high-temperature resistance of the sinorhizobium meliloti is enhanced by inoculating the genetic engineering rhizobium into a sinorhizobium meliloti plant. Compared with the prior art, the engineering bacterium constructed by the invention can effectively improve the survival and growth capability of alfalfa in a high-temperature environment, and the experimental result provides reliable technical support for breeding new stress-resistant alfalfa varieties and stable yield of ecological agriculture, and has important application value.

The application relates to application of semaglutide in preparation of a medicine for treating or delaying ovarian aging, and through a natural aging mouse model, a cell aging model and transcriptome analysis, it is first proved that semaglutide can significantly increase the number of follicles and reduce the level of aging markers, and from a molecular level, it is revealed that the mechanism of realizing multi-target point delay of ovarian aging is that semaglutide realizes multi-target point delay of ovarian aging through regulating an adipocyte cytokine signal pathway, inhibiting excessive cell division and regulating an NF-kappa B signal pathway. As a marketed drug, semaglutide has clear safety, a long half-life and high patient compliance, and has a significant clinical conversion prospect. The application breaks through the limitation that existing hormone replacement therapy and assisted reproductive technology can only treat symptoms or solve the problem of fertility, provides a first drug intervention strategy which can fundamentally delay the process of follicle depletion, and fills the blank in the field of ovarian aging treatment.

The invention provides a genetic engineering strain and a construction method thereof, the strain is a target gene deleted strain obtained by knocking out a target gene in a rod-shaped bacterial strain, and the target gene is one of minC and minD genes or a gene combination thereof. According to the present invention, the minC and / or minD gene deletion strain is obtained by knocking out the minC and / or minD gene in the rod-shaped bacterial strain, such that cell division abnormality can be caused, chromosome-free small cells are generated, and the small cells have characteristics of biological activity, no chromosome DNA, and no growth or division propagation. The small cells can generate smaller body immune response, and meanwhile, the LPS modified by the small cells can generate smaller cytotoxic reaction, so that side effects can be greatly reduced when the small cells are applied to human or animal bodies.

The invention relates to an establishment method and application of a micropterus salmoides muscle cell line, and belongs to the technical field of cell culture. The invention provides a culture solution which takes an M199 culture medium as a basic culture medium and further comprises sodium pyruvate, hydroxyethyl piperazine ethanesulfonic acid, beta-mercaptoethanol, penicillin, streptomycin and fetal calf serum. The culture solution provides optimal conditions for micropterus salmoides muscle cell culture, the culture cost is also reduced, the micropterus salmoides muscle cell line is established by adopting the culture solution, the method is simple and easy to operate and high in repeatability, the established cell line is good in stability, continuous passage and ultralow-temperature cryopreservation can be realized, the cell division speed is high, the passage time is short, the adherence rate is high, and the method is suitable for large-scale popularization and application. In the method, the micropterus salmoides muscle primary cell culture time is short, the number of obtained cells is large, the activity is good, and passage can be carried out in only 8 days.

The application discloses a kind of construction method and application of seawater fish muscle stem cell line, culture medium with basic medium as background medium;Culture medium includes LIF growth factor, EGF growth factor and leopard bristletooth-derived FGF2 protein;The final concentration of leopard bristletooth-derived FGF2 protein is 20~30 ng / mL, 30~45 ng / mL, 45~55 ng / mL or 55~70 ng / mL. Through the medium, immortalized seawater fish muscle stem cell line with good stability, cell division vigorous, short subculture time can be constructed, and can be stably subcultured for more than 50 generations. It provides raw materials for exploring the function of myoblasts, gene regulation mechanism, and provides the prerequisite for in vitro verification experiment for further exploring the influence of seawater fish muscle stem cells on growth rate, and lays a foundation for good strain breeding of seawater fish.

The invention relates to the technical field of agricultural biology, in particular to a method for increasing the germination rate of hemerocallis citrina L. seeds, the method for remarkably increasing the germination rate of the hemerocallis citrina L. seeds through combined treatment of gibberellin GA3 and hydrogen peroxide H2O2 is easy and convenient to operate and low in cost, the physiological dormancy of the seeds is broken through gibberellin, embryo cell division is promoted, and the germination rate of the seeds is increased. Meanwhile, hydrogen peroxide is used for moderately oxidizing seed coats, permeability is improved, active oxygen signal molecules are provided for activating germination related enzyme systems, the hydrogen peroxide and the active oxygen signal molecules generate a synergistic interaction effect through a dormancy breaking-permeation promoting-activation triple mechanism, the germination rate is increased in a breakthrough mode, and the germination period is remarkably shortened.

A method of producing a transformed cell or plant body of maize of the present invention includes overexpressing, in a cell or plant body of maize, 1) a nucleic acid encoding an amino acid sequence of SEQ ID NO: 2, or a nucleic acid encoding a polypeptide including an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 2, the polypeptide having a function of promoting cell division of maize, or 2) a nucleic acid encoding an amino acid sequence of SEQ ID NO: 15, or a nucleic acid encoding a polypeptide including an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 15, the polypeptide having a function of promoting cell division of maize, the overexpressing being controlled by a promoter including a 35S promoter of Cauliflower mosaic virus.

The invention relates to the use of the compound ES3707 as a therapeutic agent for the treatment of cancer diseases and for alleviating symptoms caused by such diseases, specifically by inhibiting heat shock proteins involved in cell division and by preventing resistance induced by clinical drugs. The therapeutic agent subject to the invention is characterized by its enhanced efficacy when used in combination with photodynamic therapy.

The invention discloses an FvePREs transcription factor and application thereof in promoting curling of strawberry petals, in a general agricultural cultivation technology, the petals are curled by gibberellin (GA3) or a growth regulator for actively inducing the curling of the petals, and excessive hormone may cause malformation and curling of the petals and also may cause side effects such as fruit hollowness, quality reduction and the like. According to the scheme, the gene technology is ingeniously utilized to directly act on a plant endogenous regulation and control system, the synergistic effect of strawberry petal curling and quality optimization is achieved by activating or inhibiting specific gene expression (such as cell division and nutrient distribution), and in addition, the over-expression FvePREs transcription factor can be used for improving the yield of strawberry petals by changing the hereditary characteristics of strawberries. The good petal curling effect can be kept in the long-term planting process, dependence on external chemical substances can be reduced, and sustainable agricultural development is facilitated.

The embodiment of the invention provides a golden camellia suspension cell culture method which comprises the following steps: golden camellia leaves are collected and inoculated into a WPM liquid culture medium containing hormone for culture, primary callus is formed through induction, and the hormone comprises naphthylacetic acid and 6-benayl aminopurine; and inoculating the primary callus into a WPM liquid culture medium containing auxin and cytokinin, and culturing to obtain the golden camellia suspension cells.

The invention provides a method for establishing a liver cell line of micropterus salmoides, and belongs to the technical field of cell culture. According to the invention, a tissue block method is adopted to separate from the liver tissue of the micropterus salmoides to obtain primary cells of the liver of the micropterus salmoides, and subculture is carried out to obtain the liver cell line of the micropterus salmoides. The stable and normative culture method of the largemouth bass liver cell line is established, the method is simple and easy to operate, the construction method is high in repeatability, and the constructed cell line is good in stability, exuberant in cell division and short in passage time. The method provided by the invention has important significance on development of in-vitro nutrition metabolism research, disease mechanism analysis, functional feed development and the like of the micropterus salmoides, and can also provide key technical support for healthy culture and industrial quality and efficiency improvement of the micropterus salmoides.

The invention belongs to the technical field of karyotype analysis of chromosomes, and particularly relates to a cell pretreatment solution for karyotype analysis and preparation of chromosomes and application of the cell pretreatment solution. The cell pretreatment solution comprises the following components: 15 mmol / L Hepes buffer solution with the pH value of 7.2-7.6, 0.08-1.2 [mu] g / mL of vinblastine, 0.1-0.5 v / v% of Triton X-100 and 1-2 mmol / L of EDTA (Ethylene Diamine Tetraacetic Acid), and is a cell pretreatment solution with low toxicity, better dyeing effect and good cell type universality. When the cell pretreatment liquid is used for preparing a chromosome specimen, the preparation time is remarkably shortened, not only can more metaphase cell division phases be obtained, but also the proportion of the division phases capable of being used for chromosome analysis is greater than 83%, and the efficiency of karyotype analysis is remarkably improved. Meanwhile, the prepared chromosome specimen is clear in structure number, good in form, high in dispersity and few in background impurities, and the accuracy of chromosome karyotype analysis is remarkably improved.

The application will AtGRF4 The gene is stably integrated into the cucumber genome, realizes generation inheritance, and discovers that the overexpression AtGRF4 The gene can directly activate the key path of fruit development, significantly promote fruit cell division and expansion, and directly realize fruit growth. Compared with the traditional regulation mode, the present application has important practical significance and application value for developing high-efficiency and stable cucumber fruit growth technology. The present application does not need complex equipment, is simple to operate, is suitable for existing facility cultivation system, and simultaneously AtGRF4 The function is conservative, and the stable genetic transformation technology can be expanded to other melon and fruit crops, expand the application range, give consideration to high yield and operation convenience, and meet the needs of large-scale production.

The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS / WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

The invention discloses a method for improving collagen yield of mesenchymal stem cells and engineered mesenchymal stem cells for high-yield collagen. The method comprises the following steps: designing at least one guide RNA (Ribonucleic Acid), and gRNA targets a promoter region of a collagen gene; the method comprises the following steps: introducing a composition containing gRNA and an expression vector for coding a CRISPR activation system into MSCs; forming a compound in the MSCs, specifically binding the compound to a collagen gene promoter region, and activating the transcription of the collagen gene; engineering MSCs with the expression quantity higher than that of untreated MSCs are screened and separated. The content of collagen produced by the method disclosed by the invention is obviously increased. Meanwhile, the effect is stable, the cell strain is not easy to lose along with cell division, and the yield of the screened cell strain is highly uniform and stable. More importantly, in the aspect of safety, the core CRISPRa method does not cut DNA, so that off-target damage and chromosome abnormality risks caused by DNA breakage are avoided, the potential risk is smaller, and it is ensured that the improved collagen really has biological functionality.

The invention discloses watermelon peel derived carbon dots for promoting growth and development of tomatoes as well as a preparation method and application of the watermelon peel derived carbon dots, and belongs to the technical field of plant growth regulators. The preparation method comprises the following steps: performing heat treatment on watermelon peel, grinding, fully and uniformly mixing with water, performing ultrasonic treatment and centrifugation, taking supernate, dialyzing, and filtering to obtain the watermelon peel carbon dots (WrCDs). The WrCDs induce transcription reprogramming of tomatoes, and differentiated expression of genes related to photosynthesis, energy metabolism and cell cycle regulation is caused. According to the change of the transcription level, by enhancing photosynthesis, regulating and controlling the cell cycle and promoting cell division and expansion, the growth of tomato plants is finally remarkably promoted, the flowering time of tomatoes is shortened, and the tomato fruits can appear on the market in advance.

This invention discloses a method for constructing and applying a marine fish muscle stem cell line. The culture medium uses basal medium as the background medium and contains LIF growth factor, EGF growth factor, and FGF2 protein derived from the leopard-gill sea bass. The final concentration of FGF2 protein from the leopard-gill sea bass is 20-30 ng / mL, 30-45 ng / mL, 45-55 ng / mL, or 55-70 ng / mL. Passaging using this medium can construct a stable, rapidly dividing, and short-passaging immortalized marine fish muscle stem cell line, capable of stable passage for more than 50 generations. This provides raw materials for exploring the function and gene regulation mechanisms of myoblasts, and provides in vitro verification experimental conditions for further research on the effects of marine fish muscle stem cells on growth rate, laying the foundation for the breeding of superior marine fish species.

The application relates to the technical field of genetic engineering, in particular to application of a maize MYBR47 gene in regulation of grain traits and variety selection and a transgenic plant cultivation method, wherein the sequence of the MYBR47 gene is shown as SEQ ID NO:1. A nitrogen response gene MYBR47 is found in the maize, the MYBR47 mutation can affect the grain size, starch and protein content; the yeast single hybridization result shows that the gene regulates the development of the maize grain by affecting cell division / expansion. The gene MYBR47 can be used to cultivate new varieties with different grain sizes and improved nitrogen utilization efficiency, and lays a foundation for the application practice of maize breeding.

The application discloses an application of an indole dione piperazine compound in preparation of a drug for resisting prostate cancer. Experiments such as MTT, flow cytometry, protein chip, molecular docking, CETSA, transmission electron microscopy and zebra fish model prove that the compound significantly inhibits proliferation of prostate tumor cells such as PC-3, 22Rv1 and DU145, blocks division of PC-3 cells in the G1 phase, and induces apoptosis and necrotic apoptosis of PC-3 cells; the mechanism is that the compound directly combines NQO1 and NRF2, activates the HO-1 / NRF2 / NQO1 pathway, promotes excessive generation of ROS, and causes NQO1-dependent oxidative stress. The zebra fish model in vivo shows that the compound inhibits growth of prostate tumors. The application provides a lead compound with a new mechanism for preventing and treating prostate cancer and potential for overcoming drug resistance.

The present application relates to the technical field of plant breeding, and discloses a method for efficiently inducing peony subterranean bud polyploidy and application thereof, comprising subterranean bud disinfection, cell synchronization, minimally invasive pretreatment, preparation of CS- Alg / PPy photothermal response slow-release microspheres, loading of composite inducer, microsphere implantation and photothermal regulation induction, polyploidy identification screening and transplanting, wherein the composite inducer comprises colchicine, 6-BA and graphene quantum dots, can block chromosome separation, realize cell chromosome doubling, regulate growth point cell division, simultaneously repair cell damage, improve bud survival rate, assist photothermal regulation to realize precise drug release, and promote expression of polyploid plant excellent traits. The method has high induction rate, high homozygosity and high bud survival rate, is suitable for cultivation of ornamental, cut flower, medicinal and stress-resistant peonies, and is advanced and practical in technology.

The invention relates to the field of microbial engineering, in particular to an engineering modified bacillus and a construction method and application thereof. The engineered bacterium comprises an MreB protein fused with a mutated ssrA tag protein at the C-terminal, does not express an SspB protein, and overexpresses a cell division inhibiting gene. The volume of the engineering modified bacillus provided by the embodiment of the invention can be increased to 10 times of that of a wild type in the later growth period. When the engineering modified bacillus is used for preparing biological products through fermentation, the production efficiency can be remarkably improved.

The invention discloses a preparation method of a metaphase of a porcine cell chromosome, and belongs to the technical field of genetics. The preparation method comprises the following steps: compounding colchicine and nocodazole which are microtubule polymerization inhibitors into a composition, culturing a pig cell line for 3 hours by using a culture medium containing 0.1 mu g / mL colchicine and 0.2 mu g / mL nocodazole, retarding cell division, and preparing the metaphase of the pig cell chromosome through the steps of digestion, hypotonic treatment, fixation, dropping, dyeing and the like. Experimental results show that the preparation method provided by the invention overcomes the problem of excessive condensation of chromosomes caused by single colchicine, the test efficiency is improved while the excellent morphology of the chromosomes is ensured, and the combination of colchicine and nocodazole produces an outstanding synergistic effect. The invention provides a universal, efficient and optimized method for preparing the metaphase of the porcine cell chromosome, and lays a foundation for detection and breeding of structural variation of the porcine chromosome.

The invention relates to the technical field of gene engineering, in particular to a cotton gene GhAGL15 and application thereof. The GhAGL15 gene is identified in cotton, overexpression of GhAGL15 can significantly promote embryogenic callus induction of cotton, accelerate somatic embryo formation and improve the overall regeneration efficiency, hormone level determination finds that gibberellin in a GhAGL15 knockout strain is significantly accumulated, the cytokinin level in an overexpression strain is significantly increased, and the GhAGL15 knockout strain can be used as a GhAGL15 gene. It is prompted that GhAGL15 can play a role by regulating and controlling metabolism or signal pathways of gibberellin and cytokinin by regulating and controlling far-end regulation and control areas of related genes of gibberellin and cytokinin. And a basis is provided for breeding excellent cotton varieties.

The utility model relates to the field of assisted reproductive technology equipment discloses a kind of bovine in vitro embryo culture environment simulation device, including device body assembly, the device body assembly includes simulation device body, the outer surface of the simulation device body is rotatably connected with turnover observation window, the bottom of the simulation device body is fixedly connected with buffer rod by bottom mounting seat.The utility model is through the series connection effect of first spring damper, buffer spring and second spring damper, device forms gradient shock-absorbing network in three-dimensional space, horizontal direction impact is preferentially absorbed by first spring damper and converted into heat energy dissipation, vertical load is guided to spiral spring by buffer rod and carried out flexible support, extend the action cycle to reduce peak acceleration, moving block linkage mechanism cooperates second damper to realize the vector decomposition of oblique disturbance, finally reduce the vibration amplitude at culture dish, provide nearly stationary growth interface for embryo cell division.

The present invention relates to the use of a wheat Ms2 protein in broad-spectrum inhibition of eukaryotic cell division and / or growth, and belongs to the technical field of genetic engineering. The present invention relates to a target protein Ms2, wherein a corresponding wheat CDs sequence thereof is as shown in sequence 1, and a corresponding amino acid sequence thereof is as shown in sequence 5. It is demonstrated for the first time using a transient or stable genetic transformation technique that the Ms2 protein is toxic to plant, animal and fungal cells, and inhibits cell division and / or growth thereof, thus providing theoretical support for the use of the Ms2 protein in disease resistance and gene therapy in animals and plants.

The invention discloses a traditional Chinese medicine hemagglutination rehabilitation composition for treating aplastic anemia and a preparation method thereof, and is characterized in that the traditional Chinese medicine hemagglutination rehabilitation composition is synthesized from extracts of morinda officinalis, angelica sinensis, American ginseng, rhizoma polygonati, astragalus membranaceus, cistanche, mulberry, raspberry, fructus alpiniae oxyphyllae, hawthorn, Chinese yam, fructus perillae, jujube, semen cassiae and cordyceps sinensis. Compared with the prior art, the traditional Chinese medicine composition for treating aplastic anemia disclosed by the invention has the advantages that a compound compound of a traditional Chinese medicine extract enters blood cells of a human body, mitochondria in the cells converts the compound into ATP (adenosine triphosphate) to supply energy of cell nucleuses (gene DNA), and the cell nucleuses start to split and start and stimulate bone marrow cell regeneration; the traditional Chinese medicine composition can be used for activating body dormant cells, supplementing hemoglobin and recombining stem cells, providing new blood after cell recombination for human body functions, and restarting a basic hematopoietic function of bone marrow, so that the aim of treating aplastic anemia is fulfilled.

The invention discloses a plasmid vector for stable expression of mammalian cells as well as a construction method and application of the plasmid vector, relates to the technical field of genetic engineering, and discloses a plasmid vector for stable expression of mammalian cells. The plasmid vector comprises a promoter, a polyadenylation signal, an internal ribosome entry site IRES, a replication start site, a selection marker gene and a specific gene; according to the plasmid vector for stable expression of the mammalian cells as well as the construction method and the application of the plasmid vector, an EBNA1 / oriP system is constructed, EBNA1 protein can be specifically combined with an oriP sequence on the plasmid, and the plasmid is firmly adhered to a host cell genome like an anchor and is prevented from being discharged or lost by the cells, so that a target gene can be stably inherited and expressed for a long time along with cell division, and the expression efficiency is improved. The problem of plasmid loss is solved from the source; and the plasmid vector can drive a target gene in various mammalian cells to realize efficient and stable expression of a cross-cell line, and is extremely high in universality.