132 results about "Cell division" patented technology

The invention provides a method for increasing the growth rate of a citrus breeding material, and relates to the field of agricultural science. The method for improving the growth rate of the citrus breeding material comprises the following steps: selecting high-quality seedlings, and selecting citrus seedlings with excellent hereditary characteristics as the breeding material; the soil environment is optimized, the soil structure is improved, and the soil organic matter content is increased; nutrition management: formulating a scientific fertilization scheme including the proportion and the application amount of nitrogen, phosphorus, potassium and trace elements; according to the application of the growth regulator, the plant growth regulator is used for promoting cell division and elongation; disease and pest control: combining biological control and chemical control to control the occurrence of diseases and pests, and regularly monitoring the disease and pest conditions; environment regulation and control: temperature, humidity and illumination conditions are controlled, and a suitable growth environment is created. A scientific fertilization scheme is formulated according to the soil nutrient condition and the nutritional requirements of the citrus, and sufficient nutrients are ensured to be obtained in each growth stage, so that the growth rate is remarkably increased.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a method for establishing an elymus nutans seed callus induction and regeneration system. The invention provides a tissue culture medium and a tissue culture method for culturing elymus nutans, and in the tissue culture medium provided by the invention, 2, 4-D can induce explants to form calluses and promote cell division and growth. The CH has a good effect on differentiation of embryoid and adventitious buds. ABA can promote the development of seed embryos. The KT can promote the generation of calluses and the growth of adventitious buds. NAA can promote plant growth and promote generation of adventitious roots. Under the combined action of NAA, 2, 4-D, ABA, KT and CH, the callus induction rate of the mature embryo is improved. The invention also provides a regeneration tissue culture method of elymus nutans, the mature embryo is used as an explant for tissue culture, and the callus induction rate of the mature embryo is high.

The invention discloses an application of a populus tomentosa IPT5b gene in creating a high-yield shade-tolerant populus germplasm, belongs to forest breeding, and is characterized in that a phloem specific promoter CLE41b is utilized to express a cytokinin synthetic gene IPT5b in populus tomentosa, and an overexpression material of which cambium activity and xylem development are remarkably promoted is created. After shading treatment, the number of cambium cell layers and the number of xylem cell layers of CLE41bpro: IPT5b are remarkably higher than those of wild type CLE41bpro: IPT5b, and the phenotype increases the content of cytokinin (CK) in stems, so that the wood yield of trees under the shading condition can be increased. According to the method, a high-yield and shade-tolerant new poplar variety is created in a manner of finely regulating and controlling the content of CK in the phloem of the poplar. The gene source and scientific reference are provided for improving and improving the yield and shade tolerance of other trees, and the gene has important application value in the field of forest tree gene engineering.

A biosynthesis method for preparing royal jelly acid and the use thereof in skin care, which belong to the technical field of genetic engineering and biosynthesis. The royal jelly acid is produced by means of fermentation on the basis of yeast engineering bacteria using a culture medium containing trans-2-decenoic acid. An assessment at both skin cell level and skin model level shows that royal jelly acid can promote the synthesis of stratum corneum lipids and ceramide on aging skin and repair the skin barrier; and also can promote the synthesis of mitochondria ATP and NADPH in skin fibroblasts, reduce the active oxygen content of mitochondria, improve mitochondrial dysfunctions, and inhibit apoptosis. Further experiments show that royal jelly acid can repair DNA damage of fibroblasts caused by ultraviolet irradiation, reduce telomere shortening caused by cell division and replication, and strengthen the protection of chromosomal terminal regions. The prepared royal jelly acid has wide application prospects in the development of functional skin care products for skin repairing, aging resisting, etc.

The invention relates to the field of molecular cytogenetics and potato ploidy breeding, in particular to a potato ploidy identification method for marking and counting chromosome centromere based on a potato CENH3 antibody and a ploidy detection kit. According to the method, the chromosome centromere is marked by specifically recognizing the antibody of the potato centromere nucleosome core histone CENH3 and combining the immunofluorescence technology, so that the accurate counting of the chromosome centromere is realized at the single cell level, and the ploidy of potatoes is judged. The method comprises the following steps: preparing a leaf cell suspension, sequentially incubating a potato CENH3 antibody and a labeled secondary antibody after immobilization, and observing and counting by a microscope after redyeing. The method has the advantages of high species specificity, high throughput and low cost, is not limited by special material taking parts of root tips or shoot tips and cell division periods, can accurately identify the uploid and aneuploid of potatoes, and provides an efficient tool for potato germplasm resource evaluation and cross breeding.

The invention belongs to the technical field of biological agents, and particularly relates to ginsenoside variant flavobacterium DG-16 and application thereof in relieving continuous cropping obstacles of angelica sinensis and improving the quality of the angelica sinensis. According to the ginsenoside variant flavobacterium DG-16 disclosed by the invention, the growth of angelica sinensis is promoted by increasing the contents of jasmonic acid and cytokinin hormones, reducing the contents of gibberellin and salicylic acid hormones and promoting the circulation and utilization of nitrogen; in addition, the flavobacterium ginsenoside variant DG-16 can significantly increase the chlorophyll content, is beneficial to photosynthesis of the angelica sinensis, effectively changes the physiological and biochemical states of the angelica sinensis, can resist root rot of the angelica sinensis so as to relieve successive cropping obstacles and improve the quality, and has a significant effect.

A preparation method for a micro-vehicle formed by enveloping siRNA-loaded polypeptide dendrimer nanogel with a cell membrane of engineered MSCs, and the use of same. The method comprises: using lentivirus-mediated gene transfection technology for constructing MSCs that highly express CXCR4 and PSGL-1; using cytokinin B for stimulating a cell to generate vesicles, and then performing centrifugation to collect the vesicles; by means of the electrostatic attraction between polypeptide dendrimer nanoparticles and siRNAs, attaching the siRNAs of IL-6 and HIF-1α into the polypeptide dendrimer nanoparticles; and by means of using a membrane extruder, enveloping the siRNA-loaded polypeptide dendrimer nanogel with the cell membrane. The prepared micro-vehicle exhibits a good property of aggregating at inflammatory blood vessel sites and a targeting function for joint cavities with rheumatoid arthritis. In addition, the loaded siRNAs can inhibit the expressions of IL-6 and HIF-1α in the joint cavities and inhibit the cell migration and angiogenesis. The micro-vehicle targeting inflammation sites has the advantages of excellent biocompatibility, ideal siRNA loading ratio, good inflammation targeting function, etc.

Provided is a gene ZmFIE2 for regulating maize kernel development, an encoded protein thereof, an InDel molecular marker, and a use thereof, aiming to solve the current technical problems of low maize haploid induction rate, long breeding cycle, and low maize yield. An InDel molecular marker for detecting ZmFIE2 mutants was screened out, and a primer for InDel molecular marker was designed, the InDel molecular marker and the primer thereof can be applied to the breeding of maize kernel size varieties and maize haploid or diploid lines. From the epigenetic level, it was found that ZmFIE2 regulates the development of maize embryo and endosperm, affects the accumulation of kernel storage materials, and affects the cell cycle process of endosperm cells, which has great value in improving maize yield. By using multiple created ZmFIE2 allele mutants, research has found that the loss of ZmFIE2 function can directly determine the division of endosperm cells and apomixis, which will provide new gene resources and new ways to achieve high-frequency haploid induction and heterosis fixation in maize.

The invention belongs to the technical field of veterinary medicine, and provides a co-expressed recombinant porcine alpha and gamma interferon. The I-type interferon alpha and the II-type interferon gamma not only exert the anti-virus, anti-cell division and immune regulation effects of the interferon, but also can activate T cells and natural killer cells, and fully exert the multiple activities of the interferon. The two interferons are co-expressed in insect cells, the expression protein can be folded and then processed and modified, the structure of the expression protein is closer to a natural structure, the biological activity is high, the recombinant protein is easy to purify, the production scale is easy to expand, and live viruses or potential dangerous pathogens do not need to be treated, so that the production cost is reduced; by optimizing the production process and fermentation conditions, the quality and stability of the recombinant protein can be further improved.

The invention discloses a genetically engineered bacterium for producing iturin A as well as a construction method and application of the genetically engineered bacterium. The construction method of the genetically engineered bacterium comprises the following steps: (1) knocking out bacillus amyloliquefaciens HM618 and a gamma-polyglutamic acid synthesis gene capBCA with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.7097; (2) integrating a Ph03 promoter in front of the ituDABC gene cluster; (3) knocking out spores to form regulatory genes sigE and sigF; (4) replacing an original promoter of the cell division regulation gene ezrA in situ by using a Ph03 promoter; the genetically engineered bacterium for producing the iturin A, constructed by the invention, is high in yield of the iturin A, good in strain stability and high in production intensity. And the production yield of the fermentation tank reaches 6 g / L or above. The construction method is simple and easy to operate. The method has important industrial application value.

The present invention provides a method for improving plant genetic transformation and gene editing efficiency. Specifically, the method improves the regeneration efficiency of plant genetic transformation and / or improves the efficiency of plant gene editing by expressing genes that promote plant cell division, especially meristematic cell division.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to rhizobium capable of promoting plant cell division, resisting salt and alkali and reducing alkali and application of the rhizobium. The strain number of the Neorhizobium alkalisoli is YRD01, the preservation number of the Neorhizobium alkalisoli is CCTCC NO: M 20251990, and the Neorhizobium alkalisoli is preserved in China Center for Type Culture Collection, Luojia Hill, Wuchang District, Wuhan City, Hubei Province on September 9, 2025. The strain obtained by the invention is derived from sesbania root nodules and is obtained by screening and culturing the sesbania root nodules. The rhizobium (Neorhizium alkalisol) YRD01 obtained by screening in the invention is a strain of novel rhizobium which has the characteristics of high saline-alkaline tolerance, active alkali reduction function and efficient growth promotion.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a gene RhWOX11 for promoting rhododendron callus cell division and application of the gene RhWOX11, and the nucleotide sequence of the gene RhWOX11 is as shown in SEQ ID NO.1. The amino acid sequence of the expression protein of the gene RhWOX11 is as shown in SEQ ID NO. 2. The invention also comprises application of the gene RhWOX11 for promoting the growth of the rhododendron calluses in accelerating the growth and development of the calluses, the key gene RhWOX11 for promoting the division of the rhododendron calluses is obtained through a genetic engineering means, and the key gene RhWOX11 is introduced into tobacco plant cells, so that transgenic calluses with higher division speed can be cultivated, and the application of the gene RhWOX11 for promoting the growth of the rhododendron calluses in accelerating the growth and development of the calluses is realized. Therefore, the key gene RhWOX11 can be applied to plant callus division, the success rate of callus division of plants difficult to regenerate is increased, and a technical means support is provided for large-scale breeding of seedlings.

The invention relates to a recombinant vector, genetic engineering rhizobium and application of the genetic engineering rhizobium to improvement of heat resistance of leguminous plants. The recombinant vector contains a thermal induction promoter PkatC and a cytokinin synthetic gene ipt, the nucleotide sequence of the thermal induction promoter PkatC is shown as SEQ ID NO.1, and the nucleotide sequence of the cytokinin synthetic gene ipt is shown as SEQ ID NO.2. The invention further discloses a preparation method of the recombinant vector. According to the method, a recombinant vector pSRK-PkatC-ipt containing a thermal induction promoter PkatC and a cytokinin synthetic gene ipt is transferred into sinorhizobium meliloti, genetic engineering rhizobium is constructed and obtained, and the high-temperature resistance of the sinorhizobium meliloti is enhanced by inoculating the genetic engineering rhizobium into a sinorhizobium meliloti plant. Compared with the prior art, the engineering bacterium constructed by the invention can effectively improve the survival and growth capability of alfalfa in a high-temperature environment, and the experimental result provides reliable technical support for breeding new stress-resistant alfalfa varieties and stable yield of ecological agriculture, and has important application value.

The invention relates to the technical field of biology, and particularly discloses a sample treatment method for oat karyotype detection, which comprises the following steps: S1, root tip pretreatment: taking root tip tissues after germination of oat seeds, and treating the root tip tissues in an N2O gas environment containing 0.05-0.1% colchicine for 2-3 hours, then transferring into a glacial acetic acid-ethanol mixed solution which is precooled to 4 DEG C and fixing for 15-20 minutes; s2, performing enzymolysis treatment: cutting the fixed root tip tissue to be 1-2mm, soaking the cut root tip tissue into enzymatic hydrolysate, and performing enzymolysis at 35-38 DEG C for 30-40 minutes; according to the method, the treatment time and pressure of the root tip tissue in the N2O gas environment containing 0.05%-0.1% of colchicine are controlled to be 0.5-0.8 MPa, and the treatment temperature is controlled to be 20-25 DEG C. According to the method, stagnation in the middle stage of cell division is effectively promoted, so that the dispersion effect of chromosomes is remarkably improved; the improved stationary liquid not only can effectively fix the chromosome morphology and prevent the chromosome morphology from changing in the subsequent treatment process, but also can significantly inhibit microbial contamination and prolong the preservation time of the sample.

The invention discloses a culture method of a human 293 monoclonal cell for expressing recombinant human serum albumin. The invention provides a culture method of a human 293 monoclonal cell for expressing recombinant human serum albumin. Monoclonal cells cultured by the first culture medium are fusiform, the cell adherence is tight, the cell boundary is not clear, the cell clone formation rate is high, and the division growth of the monoclonal cells is fast; after the second culture medium is replaced, standing culture is conducted to suspension culture, and finally the recombinant human serum albumin 293 monoclonal cells capable of growing in a suspension mode are obtained. The screening of human 293 monoclonal cells expressing recombinant human serum albumin from single cell cloning to suspension single cell cloning is realized, and meanwhile, a feasible method is provided for screening and culturing the monoclonal cells. Through a batch feeding culture process, the expression quantity of the recombinant human serum albumin can reach 6.3 g / L, and commercial amplification production of the recombinant human serum albumin can be supported.

The present invention belongs to the field of insect cell culture technology and discloses a method for preparing an Sf9 cell feeder layer. The method mainly comprises two steps: step S1 is subculture of Sf9 cells, in which Sf9 cells are cultured in a suitable culture medium to a suitable cell confluence; step S2 is mitomycin C treatment of the Sf9 cells, in which a specific concentration of mitomycin C is used to treat the subcultured Sf9 cells at a specific time and temperature to inhibit their cell division ability, followed by washing to remove mitomycin C and replacing with fresh culture medium. The feeder layer cells obtained by the present invention can effectively inhibit their own proliferation while maintaining good cell activity and structural integrity. They can survive stably and provide necessary support for the co-culture system. They can be used for the cultivation of insect symbiotic bacteria and other difficult-to-culture insect cells. The method is simple to operate, has mild conditions, and good reproducibility. The prepared feeder layer has a stable effect, providing a powerful tool for related research fields.

The application relates to application of semaglutide in preparation of a medicine for treating or delaying ovarian aging, and through a natural aging mouse model, a cell aging model and transcriptome analysis, it is first proved that semaglutide can significantly increase the number of follicles and reduce the level of aging markers, and from a molecular level, it is revealed that the mechanism of realizing multi-target point delay of ovarian aging is that semaglutide realizes multi-target point delay of ovarian aging through regulating an adipocyte cytokine signal pathway, inhibiting excessive cell division and regulating an NF-kappa B signal pathway. As a marketed drug, semaglutide has clear safety, a long half-life and high patient compliance, and has a significant clinical conversion prospect. The application breaks through the limitation that existing hormone replacement therapy and assisted reproductive technology can only treat symptoms or solve the problem of fertility, provides a first drug intervention strategy which can fundamentally delay the process of follicle depletion, and fills the blank in the field of ovarian aging treatment.

Disclosed herein is a custom platform called NSC-seq that can be used for cell lineage and cell division tracking in a temporal fashion but also with single-cell resolution. With simultaneous integration of single-cell transcriptomics with lineage barcoding and temporal recording information, NSC-seq facilitates the generation of multidimensional datasets for elucidating functional heterogeneity and clonal events in vivo. We apply this platform (i) to decipher lineage branching of mouse embryonic development, (ii) to record clonal dynamics of the adult intestinal epithelium, and (iii) to track clonal composition of the mouse intestinal tumors. In addition, we apply NSC-seq to assess a comprehensive gene expression phenotype for individual gene knockout at the single-cell level using existing whole-genome CRISPR knockout screening plasmid libraries. Overall, NSC-seq enables in vivo temporal recording of mammalian development and cancer at single-cell resolution.

The application discloses a bifidobacterium splitting activity detection method based on pH regulation and application thereof, and belongs to the technical field of microbial fermentation. The detection method comprises high-density fermentation of bifidobacterium with fluorescent labeling under pH regulation and splitting cycle detection through flow cytometry, and the pH regulation range is 4.30-6.30. The bifidobacterium splitting activity detection method based on pH regulation and application thereof can quickly and accurately quantitatively and qualitatively detect the real-time fermentation state of bifidobacterium. The bifidobacterium splitting activity detection method based on pH regulation can be used for quantitative evaluation of cell splitting in a complex fermentation system, and realizes bifidobacterium fermentation optimization and precise fermentation control.

The invention provides a genetic engineering strain and a construction method thereof, the strain is a target gene deleted strain obtained by knocking out a target gene in a rod-shaped bacterial strain, and the target gene is one of minC and minD genes or a gene combination thereof. According to the present invention, the minC and / or minD gene deletion strain is obtained by knocking out the minC and / or minD gene in the rod-shaped bacterial strain, such that cell division abnormality can be caused, chromosome-free small cells are generated, and the small cells have characteristics of biological activity, no chromosome DNA, and no growth or division propagation. The small cells can generate smaller body immune response, and meanwhile, the LPS modified by the small cells can generate smaller cytotoxic reaction, so that side effects can be greatly reduced when the small cells are applied to human or animal bodies.

The invention relates to an establishment method and application of a micropterus salmoides muscle cell line, and belongs to the technical field of cell culture. The invention provides a culture solution which takes an M199 culture medium as a basic culture medium and further comprises sodium pyruvate, hydroxyethyl piperazine ethanesulfonic acid, beta-mercaptoethanol, penicillin, streptomycin and fetal calf serum. The culture solution provides optimal conditions for micropterus salmoides muscle cell culture, the culture cost is also reduced, the micropterus salmoides muscle cell line is established by adopting the culture solution, the method is simple and easy to operate and high in repeatability, the established cell line is good in stability, continuous passage and ultralow-temperature cryopreservation can be realized, the cell division speed is high, the passage time is short, the adherence rate is high, and the method is suitable for large-scale popularization and application. In the method, the micropterus salmoides muscle primary cell culture time is short, the number of obtained cells is large, the activity is good, and passage can be carried out in only 8 days.

The invention relates to an engineering bacterium for producing ansamitocin as well as a construction method and application of the engineering bacterium. FtsH protein is over-expressed in the engineering bacterium. The relationship between FtsH and ansamitocin has not been researched and reported. The invention finds that the cell division protein FtsH is related to ansamitocin synthesis, the overexpression of the FtsH can improve the yield of the ansamitocin, the industrial production cost of the ansamitocin can be saved, and the fermentation efficiency can be improved. The method has important significance for realizing industrial development of antitumor drugs in China, expanding the application range and promoting clinical diagnosis and treatment of cancers.

The invention belongs to the technical field of microorganism application, and particularly relates to application of Isaria endula 2018BY-1 in production of indoleacetic acid and promotion of plant growth, the amount of indoleacetic acid secreted in a liquid LB culture medium containing L-tryptophan is 226.25 + / -25.98 mu g / mL, and the Isaria endula 2018BY-1 can effectively increase the content of indoleacetic acid in a plant environment to promote plant growth. Specifically, main root cells are induced to divide, propagate and extend, and the height of plants is increased, so that the leaf area of the plants is increased, the crop quality is improved, and the yield is increased.

The invention provides an anti-cracking agent for preventing seedless litchi fruits from cracking before picking, and particularly relates to the technical field of crop cultivation. The anti-cracking agent for preventing the seedless litchi fruits from cracking before picking comprises a component A, a component B and a component C, the component A is one of thidiazuron or forchlorfenuron, the component B adopts a brassinolide compound, the component C is a nitrate nitrogen fertilizer, and the brassinolide compound is 24-epibrassinolide. The anti-cracking composition has the beneficial effects that thidiazuron and forchlorfenuron in the anti-cracking composition are efficient plant cytokinin and can efficiently promote plant tissue cell division, brassinosteroid compounds with low concentration can remarkably promote crop growth, calcium ammonium nitrate promotes pericarp growth and increases pericarp toughness, the thidiazuron, forchlorfenuron and brassinosteroid compounds are used cooperatively, and the anti-cracking effect is good. The seed-free lychee anti-cracking agent can achieve an obvious synergistic effect on prevention of seed-free lychee cracking, can rapidly promote fruit peel cell division of fruits, enables fruit peels to grow faster, improves the toughness of the fruit peels, and is good in anti-cracking effect before picking.

The application discloses a kind of construction method and application of seawater fish muscle stem cell line, culture medium with basic medium as background medium;Culture medium includes LIF growth factor, EGF growth factor and leopard bristletooth-derived FGF2 protein;The final concentration of leopard bristletooth-derived FGF2 protein is 20~30 ng / mL, 30~45 ng / mL, 45~55 ng / mL or 55~70 ng / mL. Through the medium, immortalized seawater fish muscle stem cell line with good stability, cell division vigorous, short subculture time can be constructed, and can be stably subcultured for more than 50 generations. It provides raw materials for exploring the function of myoblasts, gene regulation mechanism, and provides the prerequisite for in vitro verification experiment for further exploring the influence of seawater fish muscle stem cells on growth rate, and lays a foundation for good strain breeding of seawater fish.

The invention relates to the technical field of agricultural biology, in particular to a method for increasing the germination rate of hemerocallis citrina L. seeds, the method for remarkably increasing the germination rate of the hemerocallis citrina L. seeds through combined treatment of gibberellin GA3 and hydrogen peroxide H2O2 is easy and convenient to operate and low in cost, the physiological dormancy of the seeds is broken through gibberellin, embryo cell division is promoted, and the germination rate of the seeds is increased. Meanwhile, hydrogen peroxide is used for moderately oxidizing seed coats, permeability is improved, active oxygen signal molecules are provided for activating germination related enzyme systems, the hydrogen peroxide and the active oxygen signal molecules generate a synergistic interaction effect through a dormancy breaking-permeation promoting-activation triple mechanism, the germination rate is increased in a breakthrough mode, and the germination period is remarkably shortened.

The present invention provides an miR 1978 inhibitor and use thereof in treating a senescence-related disease. The etiology of senescence-related diseases is proposed, and the overexpression of miR 1978 derived from the mitochondrial genome in cell line SHSY5Y is proven to give rise to decreased mRNA expression and protein levels of DHFR, KIF5C, and MSH3; as a result, SHSY5Y cell division is arrested in the G1 phase of the cell cycle, the number of cells in the G2 / M phase is significantly reduced, and meanwhile, the number of apoptotic cells is increased. Compared with a control, cells containing miR1978 have different nuclear DNA methylation states. A plurality of Alzheimer's disease-related proteins, such as APP, Tau, p-Tau, and APOE, or Parkinson's disease-related proteins, such as α-synuclein, are increased in the SHSY5Y cell line containing miR 1978, etc.

A method of producing a transformed cell or plant body of maize of the present invention includes overexpressing, in a cell or plant body of maize, 1) a nucleic acid encoding an amino acid sequence of SEQ ID NO: 2, or a nucleic acid encoding a polypeptide including an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 2, the polypeptide having a function of promoting cell division of maize, or 2) a nucleic acid encoding an amino acid sequence of SEQ ID NO: 15, or a nucleic acid encoding a polypeptide including an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 15, the polypeptide having a function of promoting cell division of maize, the overexpressing being controlled by a promoter including a 35S promoter of Cauliflower mosaic virus.

The invention relates to the technical field of gene engineering, in particular to application of a corn MYBR47 gene in regulation and control of grain traits and variety breeding and a transgenic plant cultivation method, and the sequence of the MYBR47 gene is shown as SEQ ID NO: 1. According to the application, a nitrogen response gene MYBR47 is found in corn, and MYBR47 mutation can influence the grain size, starch content and protein content; yeast one-hybridization results show that the gene regulates and controls development of corn kernels by influencing cell division / expansion. By utilizing the gene MYBR47, new varieties with different grain sizes and improved nitrogen utilization efficiency can be cultivated, and a foundation is laid for application practice of corn breeding.