110 results about "Cell division" patented technology

The invention relates to the field of molecular cytogenetics and potato ploidy breeding, in particular to a potato ploidy identification method for marking and counting chromosome centromere based on a potato CENH3 antibody and a ploidy detection kit. According to the method, the chromosome centromere is marked by specifically recognizing the antibody of the potato centromere nucleosome core histone CENH3 and combining the immunofluorescence technology, so that the accurate counting of the chromosome centromere is realized at the single cell level, and the ploidy of potatoes is judged. The method comprises the following steps: preparing a leaf cell suspension, sequentially incubating a potato CENH3 antibody and a labeled secondary antibody after immobilization, and observing and counting by a microscope after redyeing. The method has the advantages of high species specificity, high throughput and low cost, is not limited by special material taking parts of root tips or shoot tips and cell division periods, can accurately identify the uploid and aneuploid of potatoes, and provides an efficient tool for potato germplasm resource evaluation and cross breeding.

The invention belongs to the technical field of biological agents, and particularly relates to ginsenoside variant flavobacterium DG-16 and application thereof in relieving continuous cropping obstacles of angelica sinensis and improving the quality of the angelica sinensis. According to the ginsenoside variant flavobacterium DG-16 disclosed by the invention, the growth of angelica sinensis is promoted by increasing the contents of jasmonic acid and cytokinin hormones, reducing the contents of gibberellin and salicylic acid hormones and promoting the circulation and utilization of nitrogen; in addition, the flavobacterium ginsenoside variant DG-16 can significantly increase the chlorophyll content, is beneficial to photosynthesis of the angelica sinensis, effectively changes the physiological and biochemical states of the angelica sinensis, can resist root rot of the angelica sinensis so as to relieve successive cropping obstacles and improve the quality, and has a significant effect.

Provided is a gene ZmFIE2 for regulating maize kernel development, an encoded protein thereof, an InDel molecular marker, and a use thereof, aiming to solve the current technical problems of low maize haploid induction rate, long breeding cycle, and low maize yield. An InDel molecular marker for detecting ZmFIE2 mutants was screened out, and a primer for InDel molecular marker was designed, the InDel molecular marker and the primer thereof can be applied to the breeding of maize kernel size varieties and maize haploid or diploid lines. From the epigenetic level, it was found that ZmFIE2 regulates the development of maize embryo and endosperm, affects the accumulation of kernel storage materials, and affects the cell cycle process of endosperm cells, which has great value in improving maize yield. By using multiple created ZmFIE2 allele mutants, research has found that the loss of ZmFIE2 function can directly determine the division of endosperm cells and apomixis, which will provide new gene resources and new ways to achieve high-frequency haploid induction and heterosis fixation in maize.

The invention discloses a genetically engineered bacterium for producing iturin A as well as a construction method and application of the genetically engineered bacterium. The construction method of the genetically engineered bacterium comprises the following steps: (1) knocking out bacillus amyloliquefaciens HM618 and a gamma-polyglutamic acid synthesis gene capBCA with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.7097; (2) integrating a Ph03 promoter in front of the ituDABC gene cluster; (3) knocking out spores to form regulatory genes sigE and sigF; (4) replacing an original promoter of the cell division regulation gene ezrA in situ by using a Ph03 promoter; the genetically engineered bacterium for producing the iturin A, constructed by the invention, is high in yield of the iturin A, good in strain stability and high in production intensity. And the production yield of the fermentation tank reaches 6 g / L or above. The construction method is simple and easy to operate. The method has important industrial application value.

The present invention provides a method for improving plant genetic transformation and gene editing efficiency. Specifically, the method improves the regeneration efficiency of plant genetic transformation and / or improves the efficiency of plant gene editing by expressing genes that promote plant cell division, especially meristematic cell division.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to rhizobium capable of promoting plant cell division, resisting salt and alkali and reducing alkali and application of the rhizobium. The strain number of the Neorhizobium alkalisoli is YRD01, the preservation number of the Neorhizobium alkalisoli is CCTCC NO: M 20251990, and the Neorhizobium alkalisoli is preserved in China Center for Type Culture Collection, Luojia Hill, Wuchang District, Wuhan City, Hubei Province on September 9, 2025. The strain obtained by the invention is derived from sesbania root nodules and is obtained by screening and culturing the sesbania root nodules. The rhizobium (Neorhizium alkalisol) YRD01 obtained by screening in the invention is a strain of novel rhizobium which has the characteristics of high saline-alkaline tolerance, active alkali reduction function and efficient growth promotion.

The invention relates to a recombinant vector, genetic engineering rhizobium and application of the genetic engineering rhizobium to improvement of heat resistance of leguminous plants. The recombinant vector contains a thermal induction promoter PkatC and a cytokinin synthetic gene ipt, the nucleotide sequence of the thermal induction promoter PkatC is shown as SEQ ID NO.1, and the nucleotide sequence of the cytokinin synthetic gene ipt is shown as SEQ ID NO.2. The invention further discloses a preparation method of the recombinant vector. According to the method, a recombinant vector pSRK-PkatC-ipt containing a thermal induction promoter PkatC and a cytokinin synthetic gene ipt is transferred into sinorhizobium meliloti, genetic engineering rhizobium is constructed and obtained, and the high-temperature resistance of the sinorhizobium meliloti is enhanced by inoculating the genetic engineering rhizobium into a sinorhizobium meliloti plant. Compared with the prior art, the engineering bacterium constructed by the invention can effectively improve the survival and growth capability of alfalfa in a high-temperature environment, and the experimental result provides reliable technical support for breeding new stress-resistant alfalfa varieties and stable yield of ecological agriculture, and has important application value.

The invention relates to the technical field of biology, and particularly discloses a sample treatment method for oat karyotype detection, which comprises the following steps: S1, root tip pretreatment: taking root tip tissues after germination of oat seeds, and treating the root tip tissues in an N2O gas environment containing 0.05-0.1% colchicine for 2-3 hours, then transferring into a glacial acetic acid-ethanol mixed solution which is precooled to 4 DEG C and fixing for 15-20 minutes; s2, performing enzymolysis treatment: cutting the fixed root tip tissue to be 1-2mm, soaking the cut root tip tissue into enzymatic hydrolysate, and performing enzymolysis at 35-38 DEG C for 30-40 minutes; according to the method, the treatment time and pressure of the root tip tissue in the N2O gas environment containing 0.05%-0.1% of colchicine are controlled to be 0.5-0.8 MPa, and the treatment temperature is controlled to be 20-25 DEG C. According to the method, stagnation in the middle stage of cell division is effectively promoted, so that the dispersion effect of chromosomes is remarkably improved; the improved stationary liquid not only can effectively fix the chromosome morphology and prevent the chromosome morphology from changing in the subsequent treatment process, but also can significantly inhibit microbial contamination and prolong the preservation time of the sample.

The invention discloses a culture method of a human 293 monoclonal cell for expressing recombinant human serum albumin. The invention provides a culture method of a human 293 monoclonal cell for expressing recombinant human serum albumin. Monoclonal cells cultured by the first culture medium are fusiform, the cell adherence is tight, the cell boundary is not clear, the cell clone formation rate is high, and the division growth of the monoclonal cells is fast; after the second culture medium is replaced, standing culture is conducted to suspension culture, and finally the recombinant human serum albumin 293 monoclonal cells capable of growing in a suspension mode are obtained. The screening of human 293 monoclonal cells expressing recombinant human serum albumin from single cell cloning to suspension single cell cloning is realized, and meanwhile, a feasible method is provided for screening and culturing the monoclonal cells. Through a batch feeding culture process, the expression quantity of the recombinant human serum albumin can reach 6.3 g / L, and commercial amplification production of the recombinant human serum albumin can be supported.

The present invention belongs to the field of insect cell culture technology and discloses a method for preparing an Sf9 cell feeder layer. The method mainly comprises two steps: step S1 is subculture of Sf9 cells, in which Sf9 cells are cultured in a suitable culture medium to a suitable cell confluence; step S2 is mitomycin C treatment of the Sf9 cells, in which a specific concentration of mitomycin C is used to treat the subcultured Sf9 cells at a specific time and temperature to inhibit their cell division ability, followed by washing to remove mitomycin C and replacing with fresh culture medium. The feeder layer cells obtained by the present invention can effectively inhibit their own proliferation while maintaining good cell activity and structural integrity. They can survive stably and provide necessary support for the co-culture system. They can be used for the cultivation of insect symbiotic bacteria and other difficult-to-culture insect cells. The method is simple to operate, has mild conditions, and good reproducibility. The prepared feeder layer has a stable effect, providing a powerful tool for related research fields.

The application relates to application of semaglutide in preparation of a medicine for treating or delaying ovarian aging, and through a natural aging mouse model, a cell aging model and transcriptome analysis, it is first proved that semaglutide can significantly increase the number of follicles and reduce the level of aging markers, and from a molecular level, it is revealed that the mechanism of realizing multi-target point delay of ovarian aging is that semaglutide realizes multi-target point delay of ovarian aging through regulating an adipocyte cytokine signal pathway, inhibiting excessive cell division and regulating an NF-kappa B signal pathway. As a marketed drug, semaglutide has clear safety, a long half-life and high patient compliance, and has a significant clinical conversion prospect. The application breaks through the limitation that existing hormone replacement therapy and assisted reproductive technology can only treat symptoms or solve the problem of fertility, provides a first drug intervention strategy which can fundamentally delay the process of follicle depletion, and fills the blank in the field of ovarian aging treatment.

Disclosed herein is a custom platform called NSC-seq that can be used for cell lineage and cell division tracking in a temporal fashion but also with single-cell resolution. With simultaneous integration of single-cell transcriptomics with lineage barcoding and temporal recording information, NSC-seq facilitates the generation of multidimensional datasets for elucidating functional heterogeneity and clonal events in vivo. We apply this platform (i) to decipher lineage branching of mouse embryonic development, (ii) to record clonal dynamics of the adult intestinal epithelium, and (iii) to track clonal composition of the mouse intestinal tumors. In addition, we apply NSC-seq to assess a comprehensive gene expression phenotype for individual gene knockout at the single-cell level using existing whole-genome CRISPR knockout screening plasmid libraries. Overall, NSC-seq enables in vivo temporal recording of mammalian development and cancer at single-cell resolution.

The application discloses a bifidobacterium splitting activity detection method based on pH regulation and application thereof, and belongs to the technical field of microbial fermentation. The detection method comprises high-density fermentation of bifidobacterium with fluorescent labeling under pH regulation and splitting cycle detection through flow cytometry, and the pH regulation range is 4.30-6.30. The bifidobacterium splitting activity detection method based on pH regulation and application thereof can quickly and accurately quantitatively and qualitatively detect the real-time fermentation state of bifidobacterium. The bifidobacterium splitting activity detection method based on pH regulation can be used for quantitative evaluation of cell splitting in a complex fermentation system, and realizes bifidobacterium fermentation optimization and precise fermentation control.

The invention provides a genetic engineering strain and a construction method thereof, the strain is a target gene deleted strain obtained by knocking out a target gene in a rod-shaped bacterial strain, and the target gene is one of minC and minD genes or a gene combination thereof. According to the present invention, the minC and / or minD gene deletion strain is obtained by knocking out the minC and / or minD gene in the rod-shaped bacterial strain, such that cell division abnormality can be caused, chromosome-free small cells are generated, and the small cells have characteristics of biological activity, no chromosome DNA, and no growth or division propagation. The small cells can generate smaller body immune response, and meanwhile, the LPS modified by the small cells can generate smaller cytotoxic reaction, so that side effects can be greatly reduced when the small cells are applied to human or animal bodies.

The invention relates to an establishment method and application of a micropterus salmoides muscle cell line, and belongs to the technical field of cell culture. The invention provides a culture solution which takes an M199 culture medium as a basic culture medium and further comprises sodium pyruvate, hydroxyethyl piperazine ethanesulfonic acid, beta-mercaptoethanol, penicillin, streptomycin and fetal calf serum. The culture solution provides optimal conditions for micropterus salmoides muscle cell culture, the culture cost is also reduced, the micropterus salmoides muscle cell line is established by adopting the culture solution, the method is simple and easy to operate and high in repeatability, the established cell line is good in stability, continuous passage and ultralow-temperature cryopreservation can be realized, the cell division speed is high, the passage time is short, the adherence rate is high, and the method is suitable for large-scale popularization and application. In the method, the micropterus salmoides muscle primary cell culture time is short, the number of obtained cells is large, the activity is good, and passage can be carried out in only 8 days.

The invention relates to an engineering bacterium for producing ansamitocin as well as a construction method and application of the engineering bacterium. FtsH protein is over-expressed in the engineering bacterium. The relationship between FtsH and ansamitocin has not been researched and reported. The invention finds that the cell division protein FtsH is related to ansamitocin synthesis, the overexpression of the FtsH can improve the yield of the ansamitocin, the industrial production cost of the ansamitocin can be saved, and the fermentation efficiency can be improved. The method has important significance for realizing industrial development of antitumor drugs in China, expanding the application range and promoting clinical diagnosis and treatment of cancers.

The invention belongs to the technical field of microorganism application, and particularly relates to application of Isaria endula 2018BY-1 in production of indoleacetic acid and promotion of plant growth, the amount of indoleacetic acid secreted in a liquid LB culture medium containing L-tryptophan is 226.25 + / -25.98 mu g / mL, and the Isaria endula 2018BY-1 can effectively increase the content of indoleacetic acid in a plant environment to promote plant growth. Specifically, main root cells are induced to divide, propagate and extend, and the height of plants is increased, so that the leaf area of the plants is increased, the crop quality is improved, and the yield is increased.

The application discloses a kind of construction method and application of seawater fish muscle stem cell line, culture medium with basic medium as background medium;Culture medium includes LIF growth factor, EGF growth factor and leopard bristletooth-derived FGF2 protein;The final concentration of leopard bristletooth-derived FGF2 protein is 20~30 ng / mL, 30~45 ng / mL, 45~55 ng / mL or 55~70 ng / mL. Through the medium, immortalized seawater fish muscle stem cell line with good stability, cell division vigorous, short subculture time can be constructed, and can be stably subcultured for more than 50 generations. It provides raw materials for exploring the function of myoblasts, gene regulation mechanism, and provides the prerequisite for in vitro verification experiment for further exploring the influence of seawater fish muscle stem cells on growth rate, and lays a foundation for good strain breeding of seawater fish.

The invention relates to the technical field of agricultural biology, in particular to a method for increasing the germination rate of hemerocallis citrina L. seeds, the method for remarkably increasing the germination rate of the hemerocallis citrina L. seeds through combined treatment of gibberellin GA3 and hydrogen peroxide H2O2 is easy and convenient to operate and low in cost, the physiological dormancy of the seeds is broken through gibberellin, embryo cell division is promoted, and the germination rate of the seeds is increased. Meanwhile, hydrogen peroxide is used for moderately oxidizing seed coats, permeability is improved, active oxygen signal molecules are provided for activating germination related enzyme systems, the hydrogen peroxide and the active oxygen signal molecules generate a synergistic interaction effect through a dormancy breaking-permeation promoting-activation triple mechanism, the germination rate is increased in a breakthrough mode, and the germination period is remarkably shortened.

The present invention provides an miR 1978 inhibitor and use thereof in treating a senescence-related disease. The etiology of senescence-related diseases is proposed, and the overexpression of miR 1978 derived from the mitochondrial genome in cell line SHSY5Y is proven to give rise to decreased mRNA expression and protein levels of DHFR, KIF5C, and MSH3; as a result, SHSY5Y cell division is arrested in the G1 phase of the cell cycle, the number of cells in the G2 / M phase is significantly reduced, and meanwhile, the number of apoptotic cells is increased. Compared with a control, cells containing miR1978 have different nuclear DNA methylation states. A plurality of Alzheimer's disease-related proteins, such as APP, Tau, p-Tau, and APOE, or Parkinson's disease-related proteins, such as α-synuclein, are increased in the SHSY5Y cell line containing miR 1978, etc.

A method of producing a transformed cell or plant body of maize of the present invention includes overexpressing, in a cell or plant body of maize, 1) a nucleic acid encoding an amino acid sequence of SEQ ID NO: 2, or a nucleic acid encoding a polypeptide including an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 2, the polypeptide having a function of promoting cell division of maize, or 2) a nucleic acid encoding an amino acid sequence of SEQ ID NO: 15, or a nucleic acid encoding a polypeptide including an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 15, the polypeptide having a function of promoting cell division of maize, the overexpressing being controlled by a promoter including a 35S promoter of Cauliflower mosaic virus.

The invention relates to the use of the compound ES3707 as a therapeutic agent for the treatment of cancer diseases and for alleviating symptoms caused by such diseases, specifically by inhibiting heat shock proteins involved in cell division and by preventing resistance induced by clinical drugs. The therapeutic agent subject to the invention is characterized by its enhanced efficacy when used in combination with photodynamic therapy.

The invention discloses an FvePREs transcription factor and application thereof in promoting curling of strawberry petals, in a general agricultural cultivation technology, the petals are curled by gibberellin (GA3) or a growth regulator for actively inducing the curling of the petals, and excessive hormone may cause malformation and curling of the petals and also may cause side effects such as fruit hollowness, quality reduction and the like. According to the scheme, the gene technology is ingeniously utilized to directly act on a plant endogenous regulation and control system, the synergistic effect of strawberry petal curling and quality optimization is achieved by activating or inhibiting specific gene expression (such as cell division and nutrient distribution), and in addition, the over-expression FvePREs transcription factor can be used for improving the yield of strawberry petals by changing the hereditary characteristics of strawberries. The good petal curling effect can be kept in the long-term planting process, dependence on external chemical substances can be reduced, and sustainable agricultural development is facilitated.

The present invention relates to a method for the in vitro characterization and quantification of proliferative cells in an epidermal fragment, comprising the following steps: 1) Cultivating, on a support, the epidermal fragment, 2) Detaching the epidermal fragment from the support, 3) Carrying out immunolabeling on the epidermal fragment, 4) Acquiring images of the basal layer of the epidermal fragment over its entire surface, 5) reconstructing an image representing the entire basal layer of the epidermal fragment, 6) Selecting the immunolabeled nuclei of the proliferative cells by image processing, 7) Quantifying cell division by counting the nuclei by image analysis. The present invention also relates to a use of the method for evaluating the effect of a product or an active ingredient on epidermal cell regeneration. Figure 1

The embodiment of the invention provides a golden camellia suspension cell culture method which comprises the following steps: golden camellia leaves are collected and inoculated into a WPM liquid culture medium containing hormone for culture, primary callus is formed through induction, and the hormone comprises naphthylacetic acid and 6-benayl aminopurine; and inoculating the primary callus into a WPM liquid culture medium containing auxin and cytokinin, and culturing to obtain the golden camellia suspension cells.

The invention provides a method for establishing a liver cell line of micropterus salmoides, and belongs to the technical field of cell culture. According to the invention, a tissue block method is adopted to separate from the liver tissue of the micropterus salmoides to obtain primary cells of the liver of the micropterus salmoides, and subculture is carried out to obtain the liver cell line of the micropterus salmoides. The stable and normative culture method of the largemouth bass liver cell line is established, the method is simple and easy to operate, the construction method is high in repeatability, and the constructed cell line is good in stability, exuberant in cell division and short in passage time. The method provided by the invention has important significance on development of in-vitro nutrition metabolism research, disease mechanism analysis, functional feed development and the like of the micropterus salmoides, and can also provide key technical support for healthy culture and industrial quality and efficiency improvement of the micropterus salmoides.

The invention belongs to the technical field of karyotype analysis of chromosomes, and particularly relates to a cell pretreatment solution for karyotype analysis and preparation of chromosomes and application of the cell pretreatment solution. The cell pretreatment solution comprises the following components: 15 mmol / L Hepes buffer solution with the pH value of 7.2-7.6, 0.08-1.2 [mu] g / mL of vinblastine, 0.1-0.5 v / v% of Triton X-100 and 1-2 mmol / L of EDTA (Ethylene Diamine Tetraacetic Acid), and is a cell pretreatment solution with low toxicity, better dyeing effect and good cell type universality. When the cell pretreatment liquid is used for preparing a chromosome specimen, the preparation time is remarkably shortened, not only can more metaphase cell division phases be obtained, but also the proportion of the division phases capable of being used for chromosome analysis is greater than 83%, and the efficiency of karyotype analysis is remarkably improved. Meanwhile, the prepared chromosome specimen is clear in structure number, good in form, high in dispersity and few in background impurities, and the accuracy of chromosome karyotype analysis is remarkably improved.

The application will AtGRF4 The gene is stably integrated into the cucumber genome, realizes generation inheritance, and discovers that the overexpression AtGRF4 The gene can directly activate the key path of fruit development, significantly promote fruit cell division and expansion, and directly realize fruit growth. Compared with the traditional regulation mode, the present application has important practical significance and application value for developing high-efficiency and stable cucumber fruit growth technology. The present application does not need complex equipment, is simple to operate, is suitable for existing facility cultivation system, and simultaneously AtGRF4 The function is conservative, and the stable genetic transformation technology can be expanded to other melon and fruit crops, expand the application range, give consideration to high yield and operation convenience, and meet the needs of large-scale production.

The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS / WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

The present invention belongs to the technical field of chromosome karyotype analysis, and specifically discloses a chromosome karyotype analysis method and system based on deep learning. The method includes the following steps: S1, obtaining a metaphase image of a chromosome and performing preprocessing; S2, extracting a feature F from the image; S3, clipping the feature F using multiple suggestion frames to obtain features F1 to Fn; S4, removing the common features of features F1 to Fn to obtain modified features f1 to fn, and performing classification to obtain a predicted classification result, using loss functions IG Loss and CE Loss for joint supervision; S5, fine-tuning the suggestion frame and using CE Loss to supervise the fine-tuning result; S6, using binary crossover loss (BCE Loss) to supervise the predicted classification result to obtain the chromosome karyotype analysis result. Using this technical solution, chromosome karyotype analysis is completed using deep learning and segmentation frames, and the chromosome classification effect is targetedly optimized according to the characteristics of the chromosome, thereby improving overall performance.