17 results about "Bone Marrow Stem Cell" patented technology

The utility model belongs to the technical field of medical instruments, and discloses a bone marrow blood cell filtering and collecting device which comprises a support, a collecting pipe, a net barrel and a blood product bag, the collecting pipe is detachably arranged on the support and is of a barrel-shaped structure with an opening in the top, a liquid outlet connector is arranged at the bottom of the collecting pipe and detachably connected with a liquid outlet pipe, and the liquid outlet pipe is connected with the net barrel. A liquid stopping clamp is arranged on the liquid outlet pipe; the net cylinder is of a cylindrical structure with a top opening, the net cylinder is arranged in the collecting pipe, the edge of the top opening of the net cylinder is provided with a connecting part, and the connecting part is detachably connected with the top opening of the collecting pipe; the blood product bag is detachably connected with the end, away from the liquid outlet connector, of the liquid outlet pipe. According to the bone marrow blood cell filtering and collecting device, impurities in bone marrow blood can be effectively filtered out, the purposes of separating bone marrow stem cells, reducing waste of the bone marrow blood cells and conveniently transferring the bone marrow blood cells into a blood product bag by an operator are achieved, and the quality of the collected bone marrow blood cells and the hematopoietic stem cell infusion effect are improved.

The invention discloses bone marrow stem cell collection anticoagulation stirring equipment which comprises a transparent collection barrel, a temperature control module is embedded in the bottom in the transparent collection barrel, an upper cover is arranged above the transparent collection barrel, a stirring motor is fixedly connected to the upper surface of the upper cover, and filling grooves are symmetrically formed in the upper surface of the upper cover; the output end of the stirring motor is fixedly connected with a rotating frame, one end of the rotating frame is fixedly connected with a clamping lantern ring, a stirring rod is sleeved with the clamping lantern ring, the outer wall of one side of the transparent collecting barrel is fixedly connected with a threaded pouring opening, a filter head is installed on the threaded pouring opening in a threaded mode, and a plurality of filter screens are fixedly connected into the filter head. The stirring motor is started to drive the rotating frame to rotate, the rotating frame can drive the stirring rod to rotate through the clamping lantern ring, then the collected bone marrow stem cells are continuously stirred, solidification of the bone marrow stem cells is avoided, slow manual stirring is not needed, meanwhile, a machine is adopted for stirring and anticoagulation, and the situation that the stirring speed is too high or too low can be avoided.

The invention discloses a preparation method of a respiratory tract delivery preparation for severe respiratory system diseases, the respiratory tract delivery preparation comprises the following components by weight: 2 * 10 < 8 >-2 * 10 < 9 > pieces / ml of cell exosomes, normal saline with atomization concentration of 3%, and the balance of water. The cell exosome is composed of adipose-derived stem cell exosomes and bone marrow stem cell exosomes, the adipose-derived stem cell exosomes are 1 * 10 < 8 >-1 * 10 < 9 > / ml, the bone marrow stem cell exosomes are 1 * 10 < 8 >-1 * 10 < 9 > / ml, and the ratio of the adipose-derived stem cell exosomes to the bone marrow stem cell exosomes is 1: 1. The respiratory tract delivery preparation prepared by the invention can utilize nasal spray, aerosol inhalation or other suitable drug delivery technology, and the drug delivery method ensures that the preparation directly enters the respiratory system, nasal mucosa and bronchoalveolus, which generally relieves the curative effect of treating major respiratory system diseases at present. And a clinical basis is provided for research and development in a new direction of disease-targeted biological treatment.

The invention belongs to the technical field of biomedical materials, and particularly relates to collagen hydrogel loaded with gold nanoclusters and a preparation method and application thereof.The hydrogel is collagen wrapping the gold nanoclusters, the gold nanoclusters are connected with targeted polypeptide, and the targeted polypeptide is a collagen hydrogel loaded with the gold nanoclusters and a preparation method and application of the collagen hydrogel loaded with the gold nanoclusters. The hydrogel not only has a mutually communicated porous structure and relatively low swelling and enzymatic degradation resistance, but also has good mechanical strength and injectable performance; the hydrogel can intelligently respond to high-level active oxygen and regulate and control controllable release of the gold nanoclusters; the hydrogel has excellent biocompatibility and biological activity, and can remarkably promote cell proliferation and cartilage differentiation of bone marrow mesenchymal stem cells; the cartilage tissue is recognized in a targeted manner, the regeneration of the cartilage tissue is promoted, and the material can be applied to clinical treatment of osteoarthritis.

The invention relates to a method for regulating osteogenic differentiation of periodontal ligament stem cells in an inflammatory microenvironment based on a stem cell-derived extracellular matrix. The method comprises the following steps: 1) separating and identifying bone marrow cells BMCs and periodontal ligament cells PDLCs; (2) preparing a bone marrow stem cell derived extracellular matrix B-ECM and a periodontal ligament stem cell derived extracellular matrix P-ECM; 3) intervening PDLSCs in an inflammation microenvironment by using the B-ECM and the P-ECM obtained in the step 2), and 4) performing animal experiment.The adverse effect of inflammation on the osteogenic differentiation capacity of periodontal ligament stem cells in the stem cell treatment process is reversed, so that a basis is provided for developing a novel periodontitis regeneration treatment strategy based on an ECM stent.

The utility model discloses an in-vitro separation and enrichment device for bone marrow stem cells, and relates to the technical field of in-vitro separation and enrichment of stem cells. The utility model provides an in-vitro separation and enrichment device for bone marrow stem cells, which comprises a plurality of liquid storage pipes which are connected with one another and are internally provided with nylon net filter membranes with different pore diameters; the enriching structure is connected with the filtering structure, and a filter plate which is used for bearing an enriching material and is provided with holes is arranged in the enriching structure. The filter structure comprises a first liquid storage pipe, a second liquid storage pipe and a third liquid storage pipe which are detachably connected, a first filter membrane is arranged in the first liquid storage pipe, and a second filter membrane is arranged in the second liquid storage pipe; by designing the detachably connected enrichment tube, after stem cells flow into the enrichment tube, the enrichment material adsorbed with bone marrow stem cells can be conveniently taken out, and the operation is convenient.

According to the invention, auxiliary measures such as purification of accumulated lead in the body and supplementation of neurotrophic factors are created through a'diet therapy 'matching technology. Particularly, lead infecting nerve cells and myelin sheaths is removed, a nerve transmission channel and bioelectricity response capability are repaired, and hippocampus injury is avoided; meanwhile, the survival microenvironment of the bone marrow stem cells is purified, and the sufficient self-healing instinct of the human body and the bone marrow stem cells is repaired, so that the nervous system of the body can be more perfectly recuperated and recovered by depending on the self-healing system of special medicine.

The invention relates to the technical field of biological cell storage, in particular to a bone marrow stem cell cryopreservation method and device.The method comprises the steps that a bone marrow stem cell cryopreservation system is started, the bone marrow stem cell cryopreservation system comprises a stem cell screening unit, a cryoprotectant selecting unit, a cooling unit and a liquid nitrogen cryopreservation unit, and original bone marrow is confirmed; the stem cell screening unit is used for selecting the bone marrow to be screened with the highest cell survival rate to obtain bone marrow to be cryopreserved, a currently available cryoprotectant is selected from the cryoprotectant selection unit, the cryoprotectant is injected into the cooling unit, and then the bone marrow to be cryopreserved is obtained. The bone marrow cryopreservation unit is used for receiving to-be-cryopreserved bone marrow transmitted by the stem cell screening unit, cooling the to-be-cryopreserved bone marrow containing a cryoprotectant in the cooling unit to obtain cooled bone marrow, and the liquid nitrogen cryopreservation unit is used for cryopreserving the cooled bone marrow to complete cryopreservation of bone marrow stem cells. According to the invention, the intelligence of cryopreserved bone marrow stem cells can be improved.

The invention relates to the technical field of stem cell biology, in particular to a cartilage induction culture medium and application thereof in preparation of cartilage cells derived from bone marrow stem cells. The cartilage induction culture medium comprises a DMEM / F-12 culture medium, fetal calf serum, penicillin, streptomycin, sodium pyruvate, an insulin-transferrin-selenium additive, dexamethasone, L-ascorbic acid, L-proline, a transforming growth factor-beta1 and docetaxel. The cartilage induction culture medium provided by the invention can obviously improve the chondrogenic differentiation efficiency of bone marrow mesenchymal stem cells (BMSCs), is beneficial to shortening the in-vitro preparation period of bone marrow stem cell-derived cartilage cells, and avoids the loss of cartilage cell phenotypes in the stem cell in-vitro culture process to the greatest extent; the method has important application value in preparation research, development and clinical transformation of cartilage cell transplantation.

This invention discloses a 3D scaffold for repairing defective lung injury, its construction method, and its applications, belonging to the field of biomedical materials and tissue engineering technology. This invention uses cationic polysaccharide chitosan and polymer γ-polyglutamic acid as raw materials, utilizing their electrostatic interaction to achieve cross-linking without cross-linking agents, and combines this with freeze-drying technology to prepare a three-dimensional spherical γ-PGA / CS composite scaffold. The above composite scaffold is combined with bone marrow stem cell exosomes (MSC-Exos) and type II alveolar epithelial cells (RLE-6TN). By loading exosomes onto the scaffold and implanting lung cells, local enrichment and targeted delivery are achieved, solving the problems of poor targeting of bone marrow stem cell exosomes and easy loss during transplantation of individual cells. This functional scaffold can accelerate lung function recovery by regulating the inflammatory microenvironment at the site of lung injury in SD rats, promoting lung cell regeneration, and reducing secondary lung tissue damage. It provides a novel implantable, minimally invasive treatment for defective lung injury-related diseases, possessing good clinical translational potential and application prospects in organ damage repair.

The present invention relates to the field of medical technology, and provides a zinc sulfide modified hydrogel with near-infrared light responsiveness-photothermal effect, and a preparation method and application thereof. The zinc sulfide modified hydrogel provided by the present invention comprises a methacrylated gelatin hydrogel solution and zinc sulfide nanoparticles dispersed in the methacrylated gelatin (GelMA) hydrogel solution. The present invention utilizes the biological principle that low-frequency-low-heat stimulation can promote the osteogenic differentiation ability of BMSCs, introduces zinc sulfide nanoparticles into the GelMA hydrogel, and the resulting modified hydrogel has good biocompatibility, near-infrared responsiveness and photothermal effect, can promote the proliferation ability, ATP energy production ability, cell motility and osteogenic differentiation ability of bone marrow stem cells, and is expected to be applied to bone tissue damage sites under clinical conditions, providing a potentially available biological gel preparation for promoting bone tissue damage, and expanding the application of photothermal effect in bone tissue engineering.

The invention provides application of a bone marrow stem cell engineered exosome containing a BDNF gene in treatment of spinal cord injury, and belongs to the technical field of biology. Through an endogenous transfection technology, mRNA and protein of the BDNF are loaded into the exosome, and the bone marrow stem cell engineered exosome for endogenous carrying of the brain-derived neurotrophic factor BDNF is constructed for the first time. The bone marrow stem cell engineered exosome containing the BDNF gene has dual treatment effects of a natural exosome and BDNF, and can recover spinal cord tissue injury, and compared with the morphological structure of a normal spinal cord part, the recovery rate is close to 90%. Moreover, the side effects and limitations of the original drug treatment and surgical treatment are overcome, the recovery of the motion function of the spinal cord injury is remarkably promoted, a good treatment effect can still be kept on the 28th day of the spinal cord injury, and the problem that no drug or surgical method can be used for completely treating the spinal cord injury at present is solved.

The invention belongs to the field of implant coatings, and discloses a graphene oxide GO / hydroxyapatite HA / IL-4 implant coating as well as a preparation method and application thereof. According to the invention, a hydroxyapatite and graphene oxide mixed implant coating with slow-release IL-4 is constructed on the surface of titanium through an electrochemical method, and relatively perfect characterization detection is carried out on the coating. According to the invention, hydroxyapatite and graphene oxide can be combined to form an implant surface coating material, and then a proper amount of IL-4 is adsorbed through a relatively high specific surface area and is slowly and continuously released, so that polarization of macrophages towards the M2 direction can be intervened while osteogenic differentiation of bone marrow stem cells is promoted, formation of in-vivo osseointegration of the implant is accelerated, and the bone marrow stem cells can be effectively treated. And osteogenesis is promoted. The application of the GO / HA / IL-4 coating enables the implant to have wider indications, the success rate is improved, the treatment process is shortened, and the healing time is shortened.

The invention discloses an application of a Cbf [beta] gene in preparation of a medicine for treating fracture nonunion, which is characterized in that the Cbf [beta] gene is overexpressed by targeting bone marrow adipocytes, so that the effects of maintaining the homeostasis of skeletal stem cells and promoting bone repair of Cbf [beta] protein in bone marrow adipocytes and adipogenic precursor cells (such as Adipoq + cells for short) are recovered or enhanced, and the bone fracture nonunion is improved. The bone marrow microenvironment is effectively improved, the steady state of skeletal stem cells is recovered, the function of the aged bone marrow fat cells can be directly improved, and then bone regeneration and fracture repair are promoted. The invention not only overcomes the defect of limited curative effect of the existing operation, transplantation and repair materials in the aged patients, but also has the advantages of high safety, strong targeting property, high efficiency and the like, and has obvious application value and wide clinical prospect in the treatment of aging fracture nonunion.

The utility model relates to the technical field of cell separation, in particular to separation equipment for bone marrow stem cell extraction, which comprises a rotary drum, a stem cell separation bottle arranged on the rotary drum and a driving part for driving the rotary drum to rotate, a hand lifting part is arranged on the outer wall of the upper end of the rotary drum, and the rotary drum is detachably arranged on the driving part through the hand lifting part. The driving part is provided with a circumferential limiting assembly for driving the rotating drum to rotate, the circumferential limiting assembly is provided with a clamping assembly for limiting the axial movement of the rotating drum, the outer circumferential wall of the rotating drum is provided with a sleeving drum, the upper end of the stem cell separation bottle is provided with a quick release assembly, and the stem cell separation bottle is arranged on the sleeving drum in a sleeving mode through the quick release assembly. According to the stem cell separation bottle disclosed by the utility model, the stem cell separation bottle is convenient to disassemble and assemble through the circumferential limiting assembly, the clamping assembly and the quick disassembly assembly, so that the stem cell separation bottle is more tightly mounted.

The utility model relates to the technical field of cell culture operation machinery, in particular to a pair of tissue scissors for separating bone marrow stem cells, which comprises a scissors main body and a stabilizing mechanism, the scissor main body comprises a first scissor head, a first rod part and a first handle part which are connected in sequence, and a second scissor head, a second rod part and a second handle part which are connected in sequence, and the first rod part and the second rod part are movably connected in a crossed manner; the stabilizing mechanism comprises a stabilizing sliding sleeve and a first supporting leg, the stabilizing sliding sleeve is installed on the first rod part, the first supporting leg is arranged at the bottom of the stabilizing sliding sleeve, and when the first supporting leg is stably supported on the working face, the first scissor head and the second scissor head are suspended. According to the device, when an experimenter pauses to use the tissue scissors, the cutting edge part (scissor head) of the tissue scissors can be in a suspended state to keep clean and sanitary, so that the working efficiency of the experimenter is improved.

The invention belongs to the technical field of cell biology and tissue engineering, and particularly relates to a bone marrow mesenchymal stem cell and neuron three-dimensional contact co-culture system and a construction method thereof. According to the method, the bone marrow mesenchymal stem cells and the neurons are inoculated on the polylactic acid-glycolic acid porous cell scaffold for co-culture, so that the three-dimensional contact state of the bone marrow mesenchymal stem cells and the neurons during intracerebral cell transplantation can be better simulated, the contact area of the bone marrow mesenchymal stem cells and the neurons is increased, an in-vitro intercellular contact model is established, and the in-vitro intercellular contact model is established. The device is used for researching the mechanical acting force between contact cells, intercellular communication and the like. Besides, the bone marrow mesenchymal stem cells are marked by green fluorescent protein, so that the bone marrow mesenchymal stem cells can be distinguished from neurons under a fluorescence microscope, and the bone marrow mesenchymal stem cells can be easily separated from mixed cells of the bone marrow mesenchymal stem cells and the neurons, so that a foundation is laid for subsequent research.