5 results about "Bone Marrow Stem Cell" patented technology

The invention discloses bone marrow stem cell collection anticoagulation stirring equipment which comprises a transparent collection barrel, a temperature control module is embedded in the bottom in the transparent collection barrel, an upper cover is arranged above the transparent collection barrel, a stirring motor is fixedly connected to the upper surface of the upper cover, and filling grooves are symmetrically formed in the upper surface of the upper cover; the output end of the stirring motor is fixedly connected with a rotating frame, one end of the rotating frame is fixedly connected with a clamping lantern ring, a stirring rod is sleeved with the clamping lantern ring, the outer wall of one side of the transparent collecting barrel is fixedly connected with a threaded pouring opening, a filter head is installed on the threaded pouring opening in a threaded mode, and a plurality of filter screens are fixedly connected into the filter head. The stirring motor is started to drive the rotating frame to rotate, the rotating frame can drive the stirring rod to rotate through the clamping lantern ring, then the collected bone marrow stem cells are continuously stirred, solidification of the bone marrow stem cells is avoided, slow manual stirring is not needed, meanwhile, a machine is adopted for stirring and anticoagulation, and the situation that the stirring speed is too high or too low can be avoided.

The invention relates to the technical field of biological cell storage, in particular to a bone marrow stem cell cryopreservation method and device.The method comprises the steps that a bone marrow stem cell cryopreservation system is started, the bone marrow stem cell cryopreservation system comprises a stem cell screening unit, a cryoprotectant selecting unit, a cooling unit and a liquid nitrogen cryopreservation unit, and original bone marrow is confirmed; the stem cell screening unit is used for selecting the bone marrow to be screened with the highest cell survival rate to obtain bone marrow to be cryopreserved, a currently available cryoprotectant is selected from the cryoprotectant selection unit, the cryoprotectant is injected into the cooling unit, and then the bone marrow to be cryopreserved is obtained. The bone marrow cryopreservation unit is used for receiving to-be-cryopreserved bone marrow transmitted by the stem cell screening unit, cooling the to-be-cryopreserved bone marrow containing a cryoprotectant in the cooling unit to obtain cooled bone marrow, and the liquid nitrogen cryopreservation unit is used for cryopreserving the cooled bone marrow to complete cryopreservation of bone marrow stem cells. According to the invention, the intelligence of cryopreserved bone marrow stem cells can be improved.

This invention discloses a 3D scaffold for repairing defective lung injury, its construction method, and its applications, belonging to the field of biomedical materials and tissue engineering technology. This invention uses cationic polysaccharide chitosan and polymer γ-polyglutamic acid as raw materials, utilizing their electrostatic interaction to achieve cross-linking without cross-linking agents, and combines this with freeze-drying technology to prepare a three-dimensional spherical γ-PGA / CS composite scaffold. The above composite scaffold is combined with bone marrow stem cell exosomes (MSC-Exos) and type II alveolar epithelial cells (RLE-6TN). By loading exosomes onto the scaffold and implanting lung cells, local enrichment and targeted delivery are achieved, solving the problems of poor targeting of bone marrow stem cell exosomes and easy loss during transplantation of individual cells. This functional scaffold can accelerate lung function recovery by regulating the inflammatory microenvironment at the site of lung injury in SD rats, promoting lung cell regeneration, and reducing secondary lung tissue damage. It provides a novel implantable, minimally invasive treatment for defective lung injury-related diseases, possessing good clinical translational potential and application prospects in organ damage repair.

The invention discloses an application of a Cbf [beta] gene in preparation of a medicine for treating fracture nonunion, which is characterized in that the Cbf [beta] gene is overexpressed by targeting bone marrow adipocytes, so that the effects of maintaining the homeostasis of skeletal stem cells and promoting bone repair of Cbf [beta] protein in bone marrow adipocytes and adipogenic precursor cells (such as Adipoq + cells for short) are recovered or enhanced, and the bone fracture nonunion is improved. The bone marrow microenvironment is effectively improved, the steady state of skeletal stem cells is recovered, the function of the aged bone marrow fat cells can be directly improved, and then bone regeneration and fracture repair are promoted. The invention not only overcomes the defect of limited curative effect of the existing operation, transplantation and repair materials in the aged patients, but also has the advantages of high safety, strong targeting property, high efficiency and the like, and has obvious application value and wide clinical prospect in the treatment of aging fracture nonunion.

The utility model relates to the technical field of cell culture operation machinery, in particular to a pair of tissue scissors for separating bone marrow stem cells, which comprises a scissors main body and a stabilizing mechanism, the scissor main body comprises a first scissor head, a first rod part and a first handle part which are connected in sequence, and a second scissor head, a second rod part and a second handle part which are connected in sequence, and the first rod part and the second rod part are movably connected in a crossed manner; the stabilizing mechanism comprises a stabilizing sliding sleeve and a first supporting leg, the stabilizing sliding sleeve is installed on the first rod part, the first supporting leg is arranged at the bottom of the stabilizing sliding sleeve, and when the first supporting leg is stably supported on the working face, the first scissor head and the second scissor head are suspended. According to the device, when an experimenter pauses to use the tissue scissors, the cutting edge part (scissor head) of the tissue scissors can be in a suspended state to keep clean and sanitary, so that the working efficiency of the experimenter is improved.