16 results about "Neural stem cell" patented technology

The present invention relates to a composition for preventing, alleviating, or treating neuronal aging-related diseases or disorders, the composition comprising extracellular vesicles derived from three-dimensional spheroid-type cell aggregates. The extracellular vesicles according to the present invention can reduce the expression of aging-related proteins and induce reactivation of aged neural stem cells, and thus can be used to treat various neuronal aging-related diseases or disorders by preventing, alleviating, or treating the aging of neurons.

The application discloses a method for differentiating stem cells into caudal serotonergic neurons, a complete culture medium and application. Specifically, a new culture medium is formed by adding small molecule compounds, so that human pluripotent stem cells are gradually induced to obtain hindbrain neural stem cells, ventral hindbrain caudal neural stem cells, serotonergic precursor cells and caudal serotonergic neurons of the hindbrain. The culture method is simple, and can efficiently obtain mature serotonergic neurons of specific regions, thereby providing an effective cell model for research on diseases related to the serotonergic system.

This invention provides a pH-responsive immunomodulatory microneedle patch, its preparation method, and its application, belonging to the field of biomedical materials technology. This invention incorporates Zif-8 nanoparticles loaded with ChABC into GelMA hydrogel to prepare a pH-responsive immunomodulatory microneedle patch (Z@C-MN). As the patch gradually degrades, the system can continuously release Zn in a pH-responsive manner. 2+ Z@C-MN, along with ChABC, promotes in situ repair of cerebral fibrosis (SCI) after injury. Z@C-MN induces macrophage polarization towards a pro-regenerative phenotype, thereby downregulating the inflammatory response, increasing neurite length, and promoting the differentiation of neural stem cells into neurons. In vivo, Z@C-MN reduced the inflammatory microenvironment after injury and promoted angiogenesis in a rat SCI model. Furthermore, it activated axonal regeneration by inhibiting scar tissue formation and regulating CD4+. + T cell transformation supports neurogenesis.

The application discloses an application of miR-345-3p inhibitor in preparation of a product for promoting differentiation of neural stem cells in vitro, and belongs to the technical field of biological medicine. The application obtains miRNAs differentially expressed in a neuron differentiation process through GEO2R analysis, and miR-345-3p is one of the miRNAs significantly underexpressed; then the expression of miR-345-3p is down-regulated by using a miRNA inhibitor, and the result shows that the miR-345-3p inhibitor can promote differentiation of neural stem cells into neurons in vitro P <0.05), and underexpression of miR-345-3p can obviously promote differentiation of neural stem cells into neurons P <0.05). Therefore, a substance for promoting underexpression of miR-345-3p can be applied to preparation of a product for promoting differentiation of neural stem cells into neurons in vitro.

The application discloses an induction method for directional differentiation of neural stem cells into dopaminergic neurons, comprising the following steps: S1, activation and adhesion of NSCs; S2, directional induction of midbrain precursor cells; S3, directional induction of dopaminergic neurons; and S4, synaptic function maturation induction. The induction method for directional differentiation of neural stem cells into dopaminergic neurons can significantly improve the directional efficiency and lineage specificity of the midbrain precursor cells: by adding salvianolic acid B and icariin, and in cooperation with SHH, FGF8 and other induction factors, the midbrain lineage-specific signal pathway can be efficiently activated, the positive rate of the midbrain precursor cell marker LMX1A is increased to more than 76%, which is far better than 49% of the existing system, and abnormal differentiation of non-midbrain lineages is effectively inhibited.

The application provides a skeletal muscle organoid and a preparation method thereof, takes muscle stem cells, neural stem cells and fibroblasts as primary cells for organoid culture, restores the microenvironment of skeletal muscle as much as possible, and improves the differentiation degree of the organoid; further, the mold and frame for constructing the organoid are improved, the batch production of the organoid is realized through the array design of the PDMS mold and the polyamide frame; finally, the calcium signal video of the organoid is analyzed by using the Organblink system independently developed by the application, and the accuracy of the function evaluation result of the organoid is improved. The method provided by the application realizes the high bionics and high batch production of the skeletal muscle organoid, has low cost, and greatly promotes the development of the medical research field.

The present invention provides a novel method to isolate and expand pure neural stem cells (NSCs) from cerebrospinal fluid (CSF) of premature babies with Intraventricular haemorrhage, which produces a population enriched in NSC-CSF cells free of contaminating fibroblasts and other cell types. The present invention also includes substantially pure populations of CSF-NSC cells, and their use to treat and prevent diseases and injuries, including Intraventricular haemorrhage and post- hemorrhage hydrocephalus / developmental deficits.

The present invention relates to an animal model of cerebellar atrophy, and more specifically, to an animal model in which cerebellar atrophy occurs due to a deletion of the RaRF gene in cerebellar granule cells, neural stem cells of the central nervous system, or neural stem cells of the peripheral nervous system. The animal model of cerebellar atrophy can be used for research on the pathogenesis, diagnosis, prevention, or treatment of cerebellar atrophy, and can be used for screening therapeutic agents for cerebellar atrophy.

The application discloses a method and a model for co-culturing neural stem cells and primary neurons in vitro. The method for co-culturing neural stem cells and primary neurons in vitro comprises the following steps: 1) culturing neurons in an upper chamber or a lower chamber of a co-culturing chamber to make the primary neurons adhere to the wall, and replacing a culture solution with a co-culturing culture solution; and 2) culturing neural stem cells in the other chamber of the co-culturing chamber, wherein the neural stem cells are in the co-culturing culture solution, and co-culturing is performed, that is, the neural stem cells and the neurons are co-cultured in vitro. The method can successfully realize the co-culturing of the neural stem cells and the primary neurons in vitro, the obtained co-culturing model can well retain the biological characteristics of the two cell types, can well simulate the in-vivo growth microenvironment, and has a good application prospect.

The application discloses a delivery carrier with nucleic acid time sequence release function and a preparation method and application thereof, and belongs to the technical field of nucleic acid drug delivery. The nucleic acid time sequence release delivery carrier comprises, from inside to outside, an inner water phase layer, a middle oil phase layer and an outer water phase layer; the inner water phase layer contains an aqueous solution of nucleic acid molecules; the middle oil phase layer contains a hydrophobic segment of an active targeting nucleic acid delivery polymer and an amphiphilic block copolymer; and the outer water phase layer contains a hydrophilic segment of the amphiphilic block copolymer. After the delivery nucleic acid enters a cell, the nucleic acid molecules on the surface of the delivery carrier are released first, and then the nucleic acid molecules in the middle oil phase layer and the inner water phase are released in turn, so that the time sequence regulation or protein expression of the nucleic acid is realized. By using the technical scheme of the application, the nucleic acid is released in time sequence, the protein is regulated or expressed in a certain order in the cell, the problem that a traditional nucleic acid carrier cannot time sequence control the protein expression in the cell is overcome, and the delivery carrier can be used for preparing a drug for treating a neural stem cell gene disease.

The invention relates to the technical field related to cell culture, in particular to an induction culture medium for promoting human olfactory mucosa mesenchymal stem cells to be differentiated into neural stem cells and a culture method. According to the induction culture medium, on the basis of a basic culture medium containing nerve induction factors, chrondroitin 4-sulfate, mGluR1 activator-1 and fructus cnidii are added, so that the efficiency of induced differentiation to neural stem cells can be improved, and a cell preparation method is provided for treating neurodegenerative diseases such as Alzheimer's disease.

The purpose of the present invention is to provide a method whereby hindbrain neural stem cells can be cultured over a long period of time while maintaining multipotency. The present invention relates to a method for manufacturing hindbrain neural stem cells, the method involving: a step for inducing differentiation of hindbrain neural stem cells from pluripotent stem cells; and a step for culturing the hindbrain neural stem cells by using a culture medium that contains a GSK3β inhibitor, a TGFβ inhibitor, and a BMP inhibitor and does not contain a basic fibroblast growth factor (bFGF).

The application discloses a drug-loaded microgel capable of regulating cell behavior of neural stem cells and a preparation method thereof. The drug-loaded microgel is an ISP polypeptide-loaded microgel system; the drug-loaded microgel can regulate the stemness of neural stem cells and regulate the differentiation of neural stem cells. The preparation method is that a polymer material containing a double bond and a cell adhesion polypeptide containing a thiol group are crosslinked by ultraviolet light to prepare the microgel. Then, the microgel is freeze-dried, and the ISP polypeptide is adsorbed to prepare the drug-loaded microgel loaded with the ISP polypeptide. The prepared drug-loaded microgel is used for in-vitro culture of neural stem cells, is favorable for the maintenance of the stemness of the neural stem cells, and can promote the differentiation of the neural stem cells to neurons. The application is simple and easy to operate, and is expected to be used in in-vitro culture of neural stem cells and material design of organoids.

The application discloses a pipette for liquid replacement in the process of neural stem cell culture, and relates to the technical field of neural stem cell culture.The pipette comprises a pipe body and a rubber ball, the top of the pipe body is provided with a tapered port, the bottom of the pipe body is provided with a liquid inlet, the bottom of the rubber ball is fixedly connected with a tapered pipe, a filter screen is fixedly arranged in the liquid inlet, and the sidewall of the pipe body is provided with a cleaning mechanism for automatically cleaning the filter screen.The pipette for liquid replacement in the process of neural stem cell culture can make the culture solution enter the pipe body through the liquid inlet after being filtered by the filter screen.The arrangement of the filter screen can not only avoid the blockage of the pipette by cell clumps, but also avoid the waste of cell clumps, and ensure the culture effect.When the filter screen is blocked, the cell clumps at the bottom of the filter screen can be automatically scraped and cleaned, so that the blockage is avoided, and the efficiency and effect of pipetting are ensured.

The present invention relates to a pharmaceutical composition for preventing and treating geriatric diseases, comprising hypothalamus-like organoid-derived neural stem cells as an active ingredient. It was found that decreased memory, cognitive ability, muscle strength, muscle tissue density, and bone mineral density in aging mice due to aging were restored when the hypothalamus-like organoid-derived neural stem cells according to the present invention were transplanted into the aging mice. In addition, the transplantation of the hypothalamus-like organoid-derived neural stem cells was found to improve obesity by increasing energy metabolism. It was found that decreased memory, cognitive ability, and muscle strength in aging mice were similarly restored when exosomes isolated from the hypothalamus-like organoid-derived neural stem cells were transplanted into the aging mice. Therefore, the hypothalamus-like organoid-derived neural stem cells according to the present invention are expected to be very useful as an agent for preventing and treating geriatric diseases.

The present application relates to the technical field of biomedical materials, in particular to a composite hydrogel for promoting healing of diabetic wounds, a preparation method and application thereof. In the prior art, diabetic wounds are difficult to heal under the conditions of high glucose and high oxidative stress for a long time. The present application discloses a composite hydrogel for promoting healing of diabetic wounds, a preparation method and application thereof. The composite hydrogel is NSC / Met@HAMA, and the preparation method comprises the following steps: preparing an acrylate-metformin conjugate solution; under sterile conditions, mixing a neural stem cell suspension, a methylacrylated hyaluronic acid precursor solution and the acrylate-metformin conjugate solution at a volume ratio of 1:5:4, and performing a photo-crosslinking reaction in the presence of a photo initiator to obtain the composite hydrogel. The composite hydrogel synergistically regulates immunity and promotes vascular regeneration, and accelerates healing of diabetic wounds.