100 results about "Neural stem cell" patented technology

The invention discloses an environment-emotion gradient association memristive neural network circuit based on a TAP cell regulation mechanism. The environment-emotion gradient association memristive neural network circuit is composed of a hippocampus module and an amygdala kernel module. The hippocampus module comprises an environment recognition neuron module and an emotion generation neuron module; the environment recognition neuron module and the emotion generation neuron module are each composed of a weight regulation and control module adj based on a TAP cell regulation mechanism, an adder, a memristor, a resistor, an operational amplifier, a function module ABM1 and a sampling holder. Wherein the weight regulation and control module adj is composed of a voltage-controlled switch, a summator, a function module ABM2 and a function module ABM3. The almond kernel module is composed of a weight regulation and control module adj, a memristor, a resistor, an operational amplifier and a sampling holder. The provided circuit simulates the hippocampus and amygdala nucleus of the brain in structure, the regulation principle of TAP cells on neural stem cells is used for reference in a weight regulation mechanism, and bidirectional gradient associated memory of the environment and the emotion can be achieved.

The invention relates to the technical field of cell biology, in particular to a culture medium for inducing neural stem cells and application thereof.The culture medium for inducing the neural stem cells comprises a neural ectoderm induction culture medium and a neural stem cell induction culture medium; the neural ectoderm induction culture medium is prepared from a basic culture medium, an N-2 additive, a B-27 additive, an inhibitor SB431542 and an inhibitor Dorsomorphin, the neural stem cell induction culture medium is prepared from a basic culture medium, an N-2 additive and a B-27 additive, the basic culture medium is composed of a DMEM / F12 culture medium, a Neurobase culture medium, GlutaMax and non-essential amino acid NEAA. The problems that neural stem cells obtained through an existing single-layer adherent induction method based on small molecules or growth factors are uneven in differentiation and low in purity are solved.

The invention belongs to the technical field of biomedical materials, and particularly relates to astragaloside-loaded conductive hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: uniformly mixing a methylacrylic anhydride gelatin aqueous solution with an organic solvent solution of astragaloside, and carrying out ultraviolet light cross-linking curing to form methylacrylic anhydride gelatin / astragaloside hydrogel; soaking the obtained hydrogel in hydrochloric acid containing a free radical initiator; then soaking and polymerizing in an aniline ethanol solution; and soaking and purifying to obtain the methylacrylic anhydride gelatin-polyaniline conductive hydrogel loaded with astragaloside. In-vitro experiments prove that the compound has a certain anti-inflammatory effect, can induce neural stem cells to be directionally differentiated into neurons and oligodendrocytes, and is beneficial to promoting nerve regeneration and myelin sheath formation; finally, in-vivo experiments prove that the composition can improve the athletic ability and tissue function recovery of SCI rats, and has a good application prospect.

The present invention provides a compound with which a molecular probe capable of distinguishing NSCs from CSCs can be produced, and a method for detecting cancer stem cells using the compound. The present invention relates to a compound having a formyl group, a substrate moiety, a quenching moiety, and a luminescent moiety that is non-luminous due to the quenching moiety, wherein the luminescent moiety may be rendered luminous by detaching the substrate moiety by a substrate-degrading enzyme and also converting the formyl group to a carboxy group by an aldehyde dehydrogenase to allow the quenching moiety to dissociate.

The invention belongs to the technical field of biological medicine, and particularly relates to a single-domain antibody VHH-L1 targeting LINGO-1 protein, and the amino acid sequence of the single-domain antibody VHH-L1 is shown as SEQ ID NO: 1. The single-domain antibody has nanomole-level high affinity and excellent specificity, can effectively block the interaction between LINGO-1 and ligands thereof, and remarkably promotes differentiation and myelination of oligodendroglia cells. The invention further provides a neural stem cell subjected to genetic engineering modification, and the neural stem cell can stably and continuously secrete the single-domain antibody VHH-L1. In a spinal cord injury animal model, the engineered stem cell shows an excellent treatment effect, can significantly promote motor function recovery, axon regeneration and myelin sheath repair, and effectively inhibits glial scar formation. The double advantages of cell therapy and long-acting protein delivery are fused, and a brand new efficient treatment strategy is provided for demyelination diseases such as multiple sclerosis and spinal cord injury.

The invention provides a traditional Chinese medicine compound composition with a nerve repairing effect, a cell culture medium, a traditional Chinese medicine-neural stem cell exosome compound and application, and belongs to the technical field of biological medicine. Based on the basic theory of traditional Chinese medicine and the combined theory of traditional Chinese medicine and western medicine, the traditional Chinese medicine compound composition is provided and comprises gastrodia elata exosomes, astragalus membranaceus exosomes and resveratrol, the traditional Chinese medicine compound composition is prepared into a cell culture medium, neural stem cells are cultured through the cell culture medium, and the neural stem cells are obtained. Therefore, the traditional Chinese medicine-neural stem cell exosome compound with an efficient nerve repairing function is prepared. The traditional Chinese medicine-neural stem cell exosome compound achieves the purpose of comprehensively repairing nerve injury by precisely targeting nerve cells, promoting neurogenesis, repairing the function of a neural circuit and reducing the inflammatory effect, and can be used for preparing related medical instruments and drugs for nerve repair.

The invention relates to the field of biological medicine, and provides a neuronal cell differentiation culture medium which comprises a basic culture medium, a ROCK pathway inhibitor, a cAMP level enhancer, a neural stem cell differentiation inducer and gamma-aminobutyric acid. The neuronal induced differentiation culture medium provided by the invention is clear in component and convenient to use, and can quickly differentiate the human pluripotent stem cells into neurons with relatively mature shapes and functions. By applying the technical scheme disclosed by the invention, the problems of overlong in-vitro neuronal differentiation period, low purity of obtained neurons, immature functions and the like can be solved, and the method has very high scientific research and clinical application prospects.

The present invention relates to a composition for preventing, alleviating, or treating neuronal aging-related diseases or disorders, the composition comprising extracellular vesicles derived from three-dimensional spheroid-type cell aggregates. The extracellular vesicles according to the present invention can reduce the expression of aging-related proteins and induce reactivation of aged neural stem cells, and thus can be used to treat various neuronal aging-related diseases or disorders by preventing, alleviating, or treating the aging of neurons.

The application discloses a method for differentiating stem cells into caudal serotonergic neurons, a complete culture medium and application. Specifically, a new culture medium is formed by adding small molecule compounds, so that human pluripotent stem cells are gradually induced to obtain hindbrain neural stem cells, ventral hindbrain caudal neural stem cells, serotonergic precursor cells and caudal serotonergic neurons of the hindbrain. The culture method is simple, and can efficiently obtain mature serotonergic neurons of specific regions, thereby providing an effective cell model for research on diseases related to the serotonergic system.

This invention provides a pH-responsive immunomodulatory microneedle patch, its preparation method, and its application, belonging to the field of biomedical materials technology. This invention incorporates Zif-8 nanoparticles loaded with ChABC into GelMA hydrogel to prepare a pH-responsive immunomodulatory microneedle patch (Z@C-MN). As the patch gradually degrades, the system can continuously release Zn in a pH-responsive manner. 2+ Z@C-MN, along with ChABC, promotes in situ repair of cerebral fibrosis (SCI) after injury. Z@C-MN induces macrophage polarization towards a pro-regenerative phenotype, thereby downregulating the inflammatory response, increasing neurite length, and promoting the differentiation of neural stem cells into neurons. In vivo, Z@C-MN reduced the inflammatory microenvironment after injury and promoted angiogenesis in a rat SCI model. Furthermore, it activated axonal regeneration by inhibiting scar tissue formation and regulating CD4+. + T cell transformation supports neurogenesis.

The invention discloses a culture medium for promoting neural stem cell proliferation by using a small molecule compound and a preparation method, and belongs to the technical field of medicines. Comprising a basic culture medium and a small-molecule compound, the basic culture medium comprises the following components in parts by weight: 60-100 parts of agar, 20-40 parts of peptone, 8-12 parts of glucose, 1-3 parts of sodium chloride and 0.1-0.5 part of vitamin; the concentration of the small molecule compound in the culture medium is 20-100 [mu] M, and the small molecule compound comprises at least one of isoacteoside, vitamin P4, salvianolic acid B and 1, 2, 3, 4, 6-O-galloylglucose. The small molecule compound used in the invention activates a downstream gene expression regulatory network through specific targeting of TLX, effectively reverses decline of proliferation ability of neural stem cells, and provides a new method for in vitro amplification of high-quality neural stem cells. Meanwhile, the small molecule compounds also have a synergistic effect, and proliferation of the neural stem cells is promoted.

The invention relates to a lipid nutritional composition, a product comprising the same and application of the lipid nutritional composition in preparation of a product for promoting structural development of neural stem cells. The lipid nutritional composition comprises: docosahexaenoic acid and / or a metabolic precursor thereof; arachidonic acid and / or a metabolic precursor thereof; and a population of phospholipids consisting of phosphatidylcholine (PC) and / or a metabolic precursor thereof, phosphatidylserine (PS) and / or a metabolic precursor thereof, phosphatidylethanolamine (PE) and / or a metabolic precursor thereof, phosphatidylinositol (PI) and / or a metabolic precursor thereof, and sphingomyelin (SM) and / or a metabolic precursor thereof; wherein the ratio of the molar equivalents of arachidonic acid to the molar equivalents of docosahexaenoic acid, ARA / DHA, is 1-2: 1; and the ratio of the total molar equivalents of the phospholipid population to the molar equivalents of docosahexaenoic acid, PHL / DHA, is 1-8: 1.

The invention discloses a pharmaceutical composition for preventing and / or treating Parkinson's disease and application of the pharmaceutical composition, and relates to the technical field of biological medicine. The composition comprises nervonic acid, salvianolic acid A and pharmaceutically acceptable auxiliary materials. Experiments prove that the composition can improve intestinal flora, obviously promote alleviation of symptoms of a Parkinson's disease model animal, promote regeneration of dopaminergic neuronal cells and neural stem cells, relieve neuroinflammation, promote repair of damaged nerves, improve dyskinesia of mice caused by Parkinson's disease and improve the Parkinson's disease resistance of the mice. Therefore, the polypeptide has potential application value in treatment of the human Parkinson's disease. According to the invention, the effect of the composition is confirmed from the perspective of improving the change of intestinal flora by utilizing the relationship of the brain-intestinal axis, and a basis is provided for intervening the treatment of Parkinson's disease through the intestinal flora.

The invention discloses an enzyme response magnetic resonance imaging nanoprobe as well as a preparation method and application thereof. The enzyme response magnetic resonance imaging nanoprobe comprises a metal nanoparticle with a surface functional group A as an imaging basic unit, and a polypeptide sequence with functional groups B at two ends as a cross-linking agent and an enzyme response unit, the enzyme response magnetic resonance imaging nano-probe is a metal nano-particle cross-linked body formed by cross-linking the metal nano-particles through a polypeptide sequence. The enzyme response magnetic resonance imaging nanoprobe disclosed by the invention can be specifically cut by a high-expression enzyme in a cell differentiation process to form monodispersed metal nanoparticles, so that signal transformation is presented on magnetic resonance imaging; meanwhile, the enzyme response magnetic resonance imaging nanoprobe can establish effective association among the probe existence form, the cell state and the imaging signal, and is used for tracking the directional differentiation process of mesenchymal stem cells, neural stem cells and promyelocytic leukemia cells in real time.

The invention relates to the technical field of biological medicines, and discloses an engineered exosome preparation containing a myeloid tonifying pill extract, which is prepared from the following raw materials in parts by weight: 20 to 50 parts of exosome source cells, 5 to 90 parts of myeloid tonifying pill extract, 0.05 to 0.5 part of targeting molecules, 2 to 5 parts of trehalose, 0.5 to 2.5 parts of polyethylene glycol, 0.2 to 1 part of cholesterol and 1 to 4 parts of hyaluronic acid. The exosome source cells are one or more of neural stem cells and mesenchymal stem cells. The preparation process of the engineered exosome preparation containing the marrow supplementing pellet extract is subjected to clear and ordered step limitation, and then the whole preparation process is standardized, so that the problems that most traditional preparation processes of the preparations are not rigorous enough and have fuzzy steps, clear operation guidance is lacked, and the preparation cost is low are solved. Therefore, the medicine effect of the preparation is unstable.

The application discloses an enzyme-responsive magnetic resonance imaging nano probe and a preparation method and application thereof. The enzyme-responsive magnetic resonance imaging nano probe comprises a surface functional group A metal nanoparticle as an imaging basic unit, and a functional group B polypeptide sequence as a crosslinking agent and an enzyme-responsive unit; and the enzyme-responsive magnetic resonance imaging nano probe is a metal nanoparticle crosslinking body formed by crosslinking the metal nanoparticle through the polypeptide sequence. The enzyme-responsive magnetic resonance imaging nano probe can be specifically cut by an enzyme highly expressed in a cell differentiation process, and forms a monodispersed metal nanoparticle, so that a signal change is presented on magnetic resonance imaging. Meanwhile, the enzyme-responsive magnetic resonance imaging nano probe can establish an effective correlation among a probe existing form, a cell state and an imaging signal, and is used for real-time tracking of a directional differentiation process of mesenchymal stem cells, neural stem cells and promyelocytic leukemia cells.

The application discloses an application of miR-345-3p inhibitor in preparation of a product for promoting differentiation of neural stem cells in vitro, and belongs to the technical field of biological medicine. The application obtains miRNAs differentially expressed in a neuron differentiation process through GEO2R analysis, and miR-345-3p is one of the miRNAs significantly underexpressed; then the expression of miR-345-3p is down-regulated by using a miRNA inhibitor, and the result shows that the miR-345-3p inhibitor can promote differentiation of neural stem cells into neurons in vitro P <0.05), and underexpression of miR-345-3p can obviously promote differentiation of neural stem cells into neurons P <0.05). Therefore, a substance for promoting underexpression of miR-345-3p can be applied to preparation of a product for promoting differentiation of neural stem cells into neurons in vitro.

The application discloses an induction method for directional differentiation of neural stem cells into dopaminergic neurons, comprising the following steps: S1, activation and adhesion of NSCs; S2, directional induction of midbrain precursor cells; S3, directional induction of dopaminergic neurons; and S4, synaptic function maturation induction. The induction method for directional differentiation of neural stem cells into dopaminergic neurons can significantly improve the directional efficiency and lineage specificity of the midbrain precursor cells: by adding salvianolic acid B and icariin, and in cooperation with SHH, FGF8 and other induction factors, the midbrain lineage-specific signal pathway can be efficiently activated, the positive rate of the midbrain precursor cell marker LMX1A is increased to more than 76%, which is far better than 49% of the existing system, and abnormal differentiation of non-midbrain lineages is effectively inhibited.

Disclosed are compositions including a population of human enteric neural stem cells (ENSCs), wherein the population comprises at least one of a cell expressing nerve growth factor receptor (NGFR), a cell expressing integrin alpha 6 (ITGA6), a cell expressing L1 cell adhesion molecule (L1CAM), a cell that expresses proteolipid protein 1 (PLP1), and / or a cell that expresses paired-like homeobox 2B (PHOX2B), methods of producing the compositions, and their uses.

The invention relates to a neural stem cell exosome miR-106b compound and application of the neural stem cell exosome miR-106b compound in treatment of neurodegenerative diseases. It is found for the first time that the miR-106b-carrying neural stem cell exosome compound can effectively reduce damage to neurons and synapses and relieve neuroinflammation in in-vitro and in-vivo experiments. Animal experiment results show that in-vivo injection of the compound or the miR-106b simulant can be used as a treatment means for inhibiting neuroinflammation, and cognitive function decline related to neurodegenerative diseases can be resisted.

Use of a TGF-β small molecule inhibitor in the field of nerve regeneration, for in vitro regeneration and directed differentiation of a variety of nerve cells and brain-like organs. By adding the TGF-β small molecule inhibitor to a set of basal media having clear chemical compositions, pluripotent stem cells can be induced into adult cells derived from a variety of neural stem cells, and the number of induced nerve cells can be greatly increased.

The application belongs to the field of biomedical science and neurology, and particularly relates to application of CHCHD2 gene and a protein coded by the CHCHD2 gene in preparation of a product for promoting proliferation and differentiation of neural stem cells. The application proves that the CHCHD2 gene and the protein coded by the CHCHD2 gene are highly expressed in in-vivo neural development and in-vitro neural differentiation process; and that knocking down or over-expressing the CHCHD2 gene or the protein coded by the CHCHD2 gene has a certain regulating effect on proliferation and differentiation of neural stem cells. The application provides a new scheme for preparation of a product for promoting proliferation and differentiation of neural stem cells, and provides a new target for drug research and development of neural regeneration and repair treatment of central nervous system development deficiency diseases, neurodegenerative diseases, cerebrovascular diseases, craniocerebral and spinal cord injuries.

The invention provides an induction method for directionally differentiating neural stem cells into dopaminergic neurons. The induction method comprises the following steps: (1) electroporating the neural stem cells, and enabling a 40-80nm Fe2O3 (at) TGA (at) MIL-100 (at) PDA (at) Ag nano material to enter the neural stem cells; and (2) placing the neural stem cells in the step (1) in a 0.25-0.4 Tesla magnetic field, rotating the neural stem cells in the horizontal direction for 10-20 minutes without changing the magnetic field intensity, then culturing the neural stem cells, controlling the induced electromotive force induced by the rotating magnetic field to be 0.1-10 mV, rotating the magnetic field direction for 10-20 minutes every 23-25 hours, and culturing the neural stem cells for 72-240 hours. According to the induction method, the dopaminergic neuron differentiation rate is high, the synaptic function maturity is improved, the dopaminergic neuron differentiation rate exceeds 80%, the synaptic function maturity is improved compared with a traditional method, the induction method can be applied to Parkinson's disease treatment and nervous system injury repair, and an efficient induction technology is provided for cell treatment of nervous system degenerative diseases and injuries.

This invention discloses a culture medium and method for co-culturing neural stem cells and microglia, belonging to the field of cell co-culture technology. It includes a basal culture medium, a dual-mode liposome, and the following components: B-27 serum-free additive (vitamin A-free), GlutaMAX additive, N-acetylcysteine, and ascorbic acid-2-phosphate; wherein the dual-mode liposome includes growth factor liposomes and pH-responsive liposomes. This invention addresses the core pain point of co-culturing neural stem cells and microglia, overcoming the concentration fluctuation defects of traditional pulsed addition of free factors. It develops a dual-mode liposome delivery system, which improves the stability of cytokine action, solves the concentration fluctuation problem caused by traditional pulsed addition of free factors, achieves stable delivery, and significantly improves the survival rate of co-cultured cells.

The invention relates to a liquid dressing for skin and a preparation method of the liquid dressing. A film forming material of the liquid dressing comprises glial cell supernate. The neuroglial cells used in the invention are derived from neural stem cells which have been subjected to long-term in-vitro culture amplification, so that the obtained culture supernatant has the advantages of large scale, stable quality and mature technology, and a large number of liquid dressing products with stable and consistent quality standards can be provided in a short time; meanwhile, the supernate has good film-forming property and safety, contains a large number of human cell active components which are good in human body compatibility and beneficial to wound healing, and is suitable for large-scale batch production and application.

The invention discloses a preparation method and application of neural stem cells capable of being used for in-vivo visual neural circuit connection. The method comprises the following steps: constructing a tool virus vector which is driven by a broad-spectrum promoter EF1 alpha, is optimized by a mammal codon and is expressed by a fusion reporter gene of wheat germ lectin and tyrosinase, and transfecting the vector into neural stem cells. TYR catalyzes tyrosine in cells to synthesize melanin through a multi-step reaction, and the in-vivo visualization of a brain region connected with a neural circuit formed by transplanted neural stem cells can be realized by utilizing a nuclide labeling probe of targeted melanin. According to the method, the neural stem cells for in-vivo tracing of neural circuit connection of the neural stem cells are established for the first time, and when and where the neural stem cells are dynamically and visually traced to establish connection with the host neural network in a living body; and a powerful research tool is provided for exploring a specific brain region in which the neural stem cells form loop connection in vivo and an action mechanism of the neural stem cells in neural function repair.

The invention provides a neural stem cell induced differentiation and clinical application method for intractable epilepsy, and relates to the technical field of biological medicine, the induced differentiation method comprises the following steps: S1, cell source and preparation: adopting induced pluripotent stem cells from a patient; s2, constructing an induced differentiation system, differentiating the induced pluripotent stem cells into GABA (gamma-aminobutyric acid) inhibitory interneurons, and differentiating the GABA inhibitory interneurons; s3, purifying and amplifying the cells, adopting immunomagnetic bead sorting, and purifying and enriching the cells expressing the GABA energy neuron marker; s4, cell quality control; according to the method, the autologous induced pluripotent stem cells of the patient are taken as a source, immunological rejection is avoided, the transplanting safety is improved, and moreover, the high-purity GABAergic neurons can more effectively supplement damaged inhibitory neurons in the brain of the epileptic and reconstruct the inhibition balance of a neural network, so that the epileptic seizure is more effectively controlled.

The application provides a skeletal muscle organoid and a preparation method thereof, takes muscle stem cells, neural stem cells and fibroblasts as primary cells for organoid culture, restores the microenvironment of skeletal muscle as much as possible, and improves the differentiation degree of the organoid; further, the mold and frame for constructing the organoid are improved, the batch production of the organoid is realized through the array design of the PDMS mold and the polyamide frame; finally, the calcium signal video of the organoid is analyzed by using the Organblink system independently developed by the application, and the accuracy of the function evaluation result of the organoid is improved. The method provided by the application realizes the high bionics and high batch production of the skeletal muscle organoid, has low cost, and greatly promotes the development of the medical research field.

The invention belongs to the technical field of biological medicine, and discloses a safe and efficient non-gene editing neural stem cell and application thereof in treatment of nervous system related diseases. The invention provides a non-gene-edited neural stem cell (hCiPSC-NSCs) derived from human induced pluripotent stem cells, the neural stem cell is obtained by reprogramming human umbilical cord mesenchymal stem cells through a small molecule compound (such as a TGF-beta / GSK-3beta inhibitor), exogenous gene introduction is not involved, and the neural stem cell has a normal karyotype, no tumorigenicity and relatively high safety; and the efficient implantation of the non-tumorigenicity hippocampal region is realized in three months. The neural stem cell can improve the autism-like phenotype of a VPA-exposed rat by targeting the intestinal-brain axis at the same time.

The invention provides a neural stem cell differentiation process monitoring system for a living body and a construction method and application thereof, and belongs to the technical field of nuclear medicine molecular imaging. The invention provides a neural stem cell differentiation process monitoring system for a living body. According to the monitoring system, a TYR reporter gene is integrated into a genome of a neural stem cell through a recombinant virus vector, and the recombinant neural stem cell is obtained; after the recombinant neural stem cell is transplanted in the brain, the TYR reporter gene contained in the recombinant neural stem cell expresses tyrosinase in the recombinant neural stem cell, tyrosinase catalyzes tyrosine reaction to generate a tyrosine reaction product, and the tyrosine reaction product can be used for preparing the recombinant neural stem cell. The content of the tyrosine reaction product can be quantitatively determined through the PET probe of the targeted tyrosinase biosynthetic product, so that the in-vivo monitoring of the differentiation process of the transplanted neural stem cells in the brain is realized. The construction of the monitoring system provided by the invention provides an effective research tool for research on treatment of cerebral arterial thrombosis by regenerative medicine and neural stem cell transplantation.