16 results about "Neural crest" patented technology

Disclosed are in vitro methods for the differentiation of precursor cells into a neural crest cell (NCC) primed to a neurogenic lineage. The methods may include, for example, the steps of activating a Hedgehog signaling pathway (“HH signaling pathway”) in a precursor cell, wherein the precursor cell may be contacted with a neural crest cell induction medium for differentiation of the precursor cell into a neural crest cell. Compositions for carrying out the disclosed methods are also disclosed.

The present invention relates to a technology for developing a next-generation extracellular vesicle-based therapeutic agent and, more specifically, to a composition comprising three-dimensional stem cell-derived exosomes and neural crest cell-derived exosomes for alleviating neurological diseases. When extracellular vesicles of the present invention are administered in combination, progression of neuropathy can be effectively delayed or treated.

Provided are a preparation method for and a use of a dorsal root ganglion organoid. In one aspect, a preparation method for a dorsal root ganglion organoid is provided, which comprises: (1) inducing stem cells in a neural stem cell induction medium, to obtain neural stem cells; (2) inducing the neural stem cells in a neural crest cell induction medium, to obtain neural crest cells; (3) inducing the neural crest cells in a sensory neural progenitor cell induction medium, to obtain sensory neural progenitor cells; (4) inducing the sensory neural progenitor cells in an organoid induction medium, to obtain a dorsal root ganglion organoid. In addition, a use of the dorsal root ganglion organoid obtained by said method is also provided, for example, for drug screening or disease research.

The present invention provides a method for producing neural crest cells from pluripotent stem cells, the method comprising: a step (1) of subjecting pluripotent stem cells to suspension culture in a culture medium containing an ALK inhibitor and a bone morphogenetic protein; and (2) performing suspension culture in a culture medium containing an ALK inhibitor and a GSK-3beta inhibitor.

The presently disclosed subject matter provides for in vitro methods of inducing differentiation of stem cells into hindbrain progenitor cells, enteric neural crest lineage cells, as well as differentiated hindbrain lineage or enteric neural crest lineage cells obtained by such methods. The presently disclosed subject matter also provides for uses of such cells for preventing and / or treating enteric nervous system disorders (e.g, Hirschsprung's disease), and for screening compounds suitable for preventing and / or treating enteric nervous system disorders (e.g., Hirschsprung's disease).

Human embryonic stem cell (hESC)- and induced pluripotent stem cell (iPSC)-derived clinical-grade corneal endothelial cells (CECs) generation and cryopreservation through the neural crest cell (NCC) lineage using peripheral blood as a donor source and hESCs maximizes the availability of these vital cells for treating the corneal endothelial disease as an alternative to donor corneas for corneal endothelium (CE) transplantations and other applications.

The present invention aims to provide a culturing method and a method for producing a cell population containing a neural crest cell, the methods being able to stably mass produce the neural crest cell from a pluripotent stem cell with quality that allows the cell to be used in a therapy with a cell, and a method for producing a mesenchymal stem cell and the like using the neural crest cell. The method for selectively culturing a neural crest cell in a cell population containing a neural crest cell and / or a neural crest progenitor cell of the present invention comprises a culturing step of adherent culturing the cell population containing the neural crest cell and / or the neural crest progenitor cell, in the presence of one or more extracellular matrices selected from the group consisting of laminin with its α chain being an α1 chain and its β chain being a β1 chain, laminin with its α chain being an α2 chain and its β chain being a β1 chain, laminin with its α chain being an α2 chain and its β chain being a β2 chain, and laminin with its α chain being an α5 chain and its β chain being a β1 chain, and extracellular matrices containing integrin-binding sites of these laminins.

The invention relates to a preparation method and application of dorsal root ganglion organoid. On one hand, the invention provides the preparation method of the dorsal root ganglion organoid, which comprises the following steps: (1) inducing stem cells in a neural stem cell induction culture medium to obtain neural stem cells; (2) inducing the neural stem cells in a neural crest cell induction culture medium to obtain neural crest cells; (3) inducing the neural crest cells in a sensory nerve progenitor cell induction culture medium to obtain sensory nerve progenitor cells; and (4) inducing the sensory nerve progenitor cells in the organoid induction culture medium to obtain the dorsal root ganglion organoid. Moreover, the invention also provides application of the dorsal root ganglion organoid obtained by the method, for example, the dorsal root ganglion organoid is used for drug screening or disease research.

The present invention addresses the problem of providing a simple and efficient method for inducing differentiation of pluripotent stem cells into neural crest cells.　Provided is a method for producing neural crest cells, said method comprising a step for culturing stem cells for at least 6 days under conditions in which the expression or activity of a novel protein kinase C (nPKC) and / or an atypical protein kinase C (aPKC) is suppressed or inhibited.

The present disclosure relates to a method of selectively differentiating and isolating, from pluripotent stem cells, neural crest cells having axial specification information in the body. According to the method of the present disclosure, initial neural crest cells, which correspond to an embryo neurulation stage and have not yet formed axial information in the body, can be produced, neural crest cells having specific axial information in the human body can be produced, and the cells can be specifically isolated and cultured in vitro such that various types of neural crest cells can be obtained all at once, and biological processes of neural crest cells for retaining axial information are researched to identify developmental processes of tissues and organs, and thus various cell therapeutic agents optimized for the characteristics of locations in the human body can be developed.

The present invention relates to a technology for developing a next-generation extracellular vesicle-based therapeutic agent, and relates to a composition for alleviating neurological diseases, comprising exosomes derived from neural crest cells. When the extracellular vesicles of the present invention are used, neuropathy can be effectively prevented or treated.

The present invention includes a functionalized nerve guidance conduit (NGC), methods of making neurotrophic factor-expressing neural crest stem-like cells (NCSC) and / or Schwann cell precursor-like (SCP) cells, methods of making the functionalized nerve guidance conduit, and methods of treating nerve injury using the functionalized nerve guidance conduit.

The invention discloses a method for constructing smooth muscle cells serving as a smoke disease model, and belongs to the technical field of crossing of stem cells and neuroscience. The method comprises the following steps: S1) reprogramming CD34 + cells in in-vitro PBMCs (peripheral blood mononuclear cells) of a smoke disease patient carrying RNF213p.R4810K mutation, so as to obtain induced pluripotent stem cells; s2) directionally inducing and differentiating the induced pluripotent stem cells into smooth muscle cells through a neural crest stem cell way, wherein the obtained smooth muscle cells are the smooth muscle cells capable of serving as the smoke disease model. The model can be used for researching the pathogenesis and nerve-blood vessel interaction process of smoke diseases, and can be further applied to molecular typing of diseases and in-vitro function evaluation of candidate drugs.

The invention discloses a method for detecting early neurodevelopment toxicity of pollutants based on a neural crest specific molecular marker and application, and belongs to the technical field of environmental toxicology and molecular diagnosis. The method comprises the following steps: 1) exposing an early embryo to a to-be-detected sample or pollutant; 2) detecting the change of the specific molecular marker of the neural crest and the peripheral neuron; and 3) evaluating the early neurodevelopmental toxicity according to the marker expression difference. According to the method, the problems of early warning lag and low sensitivity of the traditional neural behavior endpoint (zebra fish 6dpf) are solved, the early neurotoxicity of low-concentration pollutants can be detected within 24 hours of pollutant exposure (zebra fish sox10 and prph), and the method can be used for early toxicity early warning and risk assessment in environmental monitoring and chemical risk assessment.

The invention provides a neural crest cell carrying a specific head-tail axis marker gene and a preparation method and application thereof, and relates to the technical field of biology, and the preparation method comprises the following steps: culturing stem cells to form an embryoid body; performing nerve induced differentiation on the embryoid body under the gradient driving of morphogenetic factors to form neural crest cells carrying specific head-tail axis marker genes, and promoting the formation of a neural tube-like structure with the polarity of the head-tail axis and the dorsal-abdominal axis when the embryoid body enters the nerve induced differentiation beginning by the gradient driving of morphogenetic factors. And inducing to generate neural crest cells carrying position-specific marker genes. The technical problem that neural crest cells carrying specific head-tail axis marker genes cannot be formed by an induced differentiation method in the prior art is solved.

The invention provides a craniofacial skeleton organoid controlled by sensory organs and sensory nerves and a preparation method thereof, and relates to the technical field of biologication.The preparation method comprises the steps that in a three-dimensional matrix, human pluripotent stem cells are induced through morphogenetic gradient driving to form nerve ectoderm with a nerve tube head-tail axis and a nerve tube back-abdominal axis; the neural ectoderm is respectively differentiated into a neural crest cell flow carrying head-tail axis position information, a visual cranium substrate-like structure carrying head-tail axis position information, an olfactory cranium substrate-like structure carrying head-tail axis position information and a trigeminal nerve cranium substrate-like structure carrying head-tail axis position information; and a craniofacial skeleton organ is formed under mediation of nerve cadherin. The technical problem that in the prior art, a method for forming a craniofacial skeleton organ through induction cannot form a sensory organ and a sensory nerve innervation function is solved.