Neural crest spectrum mesenchymal cell derived from pluripotent stem cells and induced differentiation method thereof

Abstract

The invention discloses a neural crest spectrum mesenchymal stem cell derived from pluripotent stem cells and an induced differentiation method thereof. The induced differentiation method of the neural crest spectrum mesenchymal stem cell comprises the following steps: dissociating the pluripotent stem cells to obtain cell aggregates, carrying out suspension culture to form embryoid bodies, replacing a culture solution with a neural crest cell culture solution, carrying out suspension culture of the embryoid bodies, collecting the embryoid bodies and replacing the culture solution every day; enabling the embryoid bodies to grow for a certain time by adhering to the wall after culturing for a certain time; digesting and collecting cells; culturing the collected cells by enabling the cells to adhere to the wall, replacing the culture solution with a mesenchymal stem cell culture solution, carrying out passage culture, then detecting the mesenchymal cell phenotype to obtain the neural crest spectrum mesenchymal cell. Through the method, the defects of heterogeneity and hybridity between adult-derived mesenchymal stem cells and other mesenchymal stem cells derived from the pluripotentstem cells of non-finite induction pathways can be solved; the prepared neural crest spectrum mesenchymal stem cell has higher immunoregulation and osteogenic differentiation capabilities; the standardized induced differentiation process is capable of ensuring that cell populations prepared between different batches have good consistency.

Description

technical field [0001] The invention belongs to the field of biology and medicine, and more specifically, the invention relates to a neural crest lineage mesenchymal stem cell derived from pluripotent stem cells and a preparation method thereof, including using special culture conditions and selection techniques. The present invention also relates to a therapeutic method using this population of neural crest lineage mesenchymal stem cells or its clinical application. Background technique [0002] Mesenchymal stem cells (MSCs) are a kind of adult stem cells. They are a large class of pluripotent cells that exist in many tissues in the adult body, such as bone marrow, umbilical cord blood, fat, and peripheral blood. They are in a quiescent state under normal conditions. When the tissue or organ where they are located is damaged, they can quickly enter a proliferative state and maintain a great proliferative potential, and differentiate into different cell types to repair the d...

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Problems solved by technology

[0010] However, the cells obtained by the methods reported in these literatures or patents do not control the differentiation path, only focus on solving the problem of the source of MSCs, and do not provide a corresponding solution for the heterogeneity of the cells, so the induced cells may be better than those derived from bone marrow. Mesenchyme is more heterogeneous; at the same time, these solutions do not focus on the function of cells, and products with poor therapeutic effects will greatly limit their application

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