A neural crest lineage pericyte derived from pluripotent stem cells and its differentiation method

Abstract

The invention discloses a neural crest lineage pericyte derived from pluripotent stem cells and a method for inducing differentiation thereof. The dissociated pluripotent stem cells are prepared into a single cell suspension, seeded in matrigel-coated orifice plates, and cultured with NCN2 The medium is changed every day; the cells are digested and collected after culturing for a certain period of time; the collected cells are adhered to the wall, and the culture medium is replaced with a pericyte-inducing medium, and the cell phenotype of the pericytes is detected after subculture. The present invention solves the shortcomings of limited sources of human cerebral vascular pericytes and the heterogeneity and mixing of pericytes derived from other unrestricted induced pluripotent stem cells, and the obtained neural crest lineage pericytes have a strong ability to maintain blood vessel stability and contraction ;Standardized induction and differentiation procedures can ensure good consistency of cell populations obtained between different batches.

Description

technical field [0001] The invention belongs to the field of biology and medicine, and more specifically, the invention relates to a neural crest lineage pericyte derived from pluripotent stem cells and a method for inducing differentiation thereof. Background technique [0002] Pericytes, also known as Rouget cells or vascular wall cells, cover the outside of microvascular endothelial cells and exist in capillaries, postcapillary venules and precapillary arterioles. In recent years, the function of pericytes has attracted more and more attention. It plays an important role in the morphogenesis and function of blood vessels, cardiovascular homeostasis, and diseases such as ischemic stroke and diabetic retinopathy. However, there is no clear definition of pericytes at present. As a compromise, it is usually judged by comprehensive location, morphology, and gene / protein expression patterns. [0003] Pericytes are ubiquitous in blood vessels. About 80% of microvessels in the b...

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Problems solved by technology

[0010] However, the pericytes obtained by the methods reported in these literatures or patents are either differentiated from mesoderm, or the differentiation path is not controlled, and only focus on solving the source of pericytes, and do not provide corresponding solutions for the different developmental origins of pericytes. Therefore, the induced cells may be highly heterogeneous; at the same time, these solutions do not focus on the function of the cells, and products with poor therapeutic effects will greatly limit their application

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