119 results about "Sonodynamic therapy" patented technology

Texaphyrins are selectively retained in diseased tissue cells. When exposed to low level, non-thermal sonodynamic agent such as ultrasound, the texaphyrin-incorporated cells are selectively destroyed. There is provided a method of sonodynamic therapy by administering an effective amount of an intracellular sensitizer to a mammal in need thereof and providing an effective amount of a sonodynamic agent.

The invention provides the application of porphyrin chemical compound which is in one of the right formulas in preparing a drug of acoustodynamic therapy and the acoustodynamic therapy. The acoustodynamic therapy includes the steps that the porphyrin chemical compound is applied to a patient, and then acoustic wave acts on the patient, wherein, the meaning of each symbol is as stated in the instruction.

The invention discloses hyaluronic acid and polydopamine-modified drug-loaded mesoporous titanium dioxide nanoparticles. On the one hand, the nanoparticles can be aggregated at tumor sites through active targeting of hyaluronic acid, the selectivity of the preparation is increased, and thus the toxic and side effects of the preparation are reduced. On the other hand, mesoporous titanium dioxide can generate singlet oxygen under the ultrasonic condition, meanwhile, polydopamine can convert light energy into heat energy under the irradiation of near-infrared laser, and the combined application of sonodynamic therapy, photothermal therapy and chemotherapy can be realized along with the release of drugs.

The present invention relates to diffuse ultrasound along with chemical agents to treat tissue, called sonodynamic therapy (SDT), and a system for treatment using SDT that comprises a whole body ensonification apparatus and control system. The whole body ensonification may reduce the chances of missing desired tissue that may not be easily detectable or may be found throughout the body. The apparatus has a plurality of diffuse ultrasound transducers for ensonifying at least part of a chamber filled with fluid and designed to accommodate a body for treatment. The person may be treated with sono-sensitive chemical agents, which may be activated when ensonified by the apparatus.

The invention relates to a method for preparing a mixed-valent-state iron doped zeolite imidazate skeleton nano-material. The method comprises the steps of simultaneously dispersing bivalent and trivalent iron salts and zinc nitrate into a methanol solvent to form an iron-zinc precursor solution, mixing a 2-methylimidazole methanol solution with the precursor solution, placing the mixture in an oil bath with the temperature of 40 DEG C to 100 DEG C, carrying out a sustained magnetic stirring reaction, and then, subjecting a product to centrifugation, washing and drying treatment, thereby obtaining the mixed-valent-state iron doped zeolite imidazate skeleton nano-material. The mixed-valent-state iron doped zeolite imidazate skeleton nano-material with relatively high yield and uniform and controllable particle size is obtained. According to the method disclosed by the invention, the total iron doped amount can reach 50% to the maximum through adjusting a ratio of bivalent iron salts totrivalent iron salts, and conversion ratios of zinc ions and iron ions can separately reach up to 78% and 60%. The method is simple and economical, and reacted mother liquor can be reused. The obtained nano-material has a relatively high active oxygen generating efficiency under ultrasonic induction, has a tremendous application value in degradation of environmental pollutants and sonodynamic therapy of tumors and can be applied to preparation of high-concentration monoatomic iron-carbon based catalysts.

The invention relates to a preparation method and application of a glucose-oxidase-modified mesoporous manganese dioxide composition, and effectively solves the problem of providing new drugs for treating tumor diseases by preparing a glucose-oxidase-modified mesoporous manganese dioxide composition so as to realize synergistic inhibition of tumor cell growth by sonodynamic therapy and starvationtherapy. The preparation method of the glucose-oxidase-modified mesoporous manganese dioxide composition comprises the following steps: modifying surfaces of mesoporous manganese dioxide particles with glucose oxidase through amide bond reaction; physically loading a small-molecular sound sensitizer in pore canals of the mesoporous manganese dioxide, thereby obtaining an internally loaded sensitizer; and then, performing covalent modification on the outer side of the internally loaded sensitizer with glucose-oxidase-modified pharmaceutical composition of mesoporous manganese dioxide nanoparticles. Particle sizes of the glucose-oxidase-modified pharmaceutical composition of the mesoporous manganese dioxide nanoparticles are 100-300 nm; and mass ratio of the glucose oxidase to the manganesedioxide is 1 to 5. The preparation method of the glucose-oxidase-modified mesoporous manganese dioxide composition disclosed by the invention is stable, reliable, and low in cost; and moreover, the glucose-oxidase-modified mesoporous manganese dioxide composition can be used as a magnetic resonance imaging contrast agent for performing real-time monitoring on tumors. Therefore, the glucose-oxidase-modified mesoporous manganese dioxide composition is an innovation in tumor treatment drugs.

The invention discloses application of non-surrounding displaced phthalocyanine zinc in preparing sonosensitizer, in particular discloses the non-surrounding displaced phthalocyanine zinc used as sonosensitizer in preparing sonodynamic therapy medicines. The non-surrounding displaced phthalocyanine zinc provided by the invention has a better biological compatibility, better biological selectivityand higher stability; and the preparation is simple, the operation is easy, synthetic raw materials are easy to obtain, and industrialization is easy to realize.

The invention relates to a preparation method and applications of a mesoporous calcium carbonate medicinal composition modified by hyaluronic acid, for effectively simultaneously realizing targeting, controlled release and ultrasonic therapy and ultrasonic imaging combination, and further realizing diagnosis and treatment integration on cancer. The technical scheme is as follows: the preparation method comprises the following steps: modifying hyaluronic acid on aminated calcium carbonate obtained by reacting mesoporous calcium carbonate and 3-aminopropyl triethoxysilane, and then loading a micromolecular sonosensitizer, thus obtaining the mesoporous calcium carbonate medicinal composition modified by hyaluronic acid. The preparation method has the advantages that a preparation process is simple, a method is stable and reliable, the production cost is low, and the prepared mesoporous calcium carbonate modified by hyaluronic acid and the medicinal composition of mesoporous calcium carbonate simultaneously have the efficacies of tumor targeting, in vitro and in vivo double-stimulation (in acid environment and under high-intensity focused ultrasonic condition) sensitive medicine release, sonodynamic therapy and ultrasonic imaging combined diagnosis and treatment integration, belong to an innovation in tumor treatment medicines, and have enormous economic and social benefits.

The invention relates to a chlorin and artesunate compound with photosensitive and sound-sensitive activity as well as a preparation method and an application of the compound and belongs to the technical field of chemical medicines. The chlorin and artesunate compound with photosensitive and sound-sensitive activity has different degrees of inhibition effects on human hepatoma cells Hep G2 in in-vitro anti-tumor activity evaluation. The photoactivity and ultrasonic activity are both higher than that of chlorin e6 and artesunate which are used as positive controls. The compound can be used for preparing a photosensitizer and a sound-sensitive agent in photodynamic therapy and sonodynamic therapy methods for tumor therapy.

The invention relates to methods of sonodynamic therapy comprising the co-administration of a microbubble-chemotherapeutic agent complex together with a microbubble-sonosensitiser complex. It further relates to pharmaceutical compositions comprising these complexes and their use in methods of sonodynamic therapy and / or sonodynamic diagnosis. Such methods find particular use in the treatment of cancer, e.g. pancreatic cancer.

The invention relates to a hydrophilic chlorin photo-sensitive and sono-sensitive agent with long wavelength and a preparation method and an application thereof, and belongs to the field of chemical medicine. The compound is represented as the following formula described in the description. The compound of hydrophilic chlorin photo-sensitive and sono-sensitive agent with long wavelength has inhibition effects in different degrees on human liver cancer cell Hep G2 in anticancer activity evaluation in vitro. The ratio of photoactivity to dark activity and the ratio of sono-activity to dark activity are much higher than that of hemoporphyrinmonomethyl ether as a positive contrast. The hydrophilic chlorin photo-sensitive and sono-sensitive agent with long wavelength has advantages of low toxicity, high efficiency, treatment wavelength moving towards long wave region and the like. The compound in the invention can be used as an excellent photosensitizer and an excellent sonosensitizer for photodynamic and sonodynamic therapy of tumors.

The invention discloses a polyamino acid sound-sensitive agent and a preparation method and application thereof. A main chain structure of the polyamino acid sound-sensitive agent is aspartic acid grafted with protoporphyrin, the aspartic acid has good biocompatibility, modifying a water-soluble polyaspartic acid side bond with the protoporphyrin greatly improves the solubility of the protoporphyrin, and the blood compatibility in a body and the accumulation quantity on a focus portion of the sound-sensitive agent are improved, so that a good treatment effect is generated, and efficient safe tumor treatment is achieved. The invention further provides a sound-sensitive agent nanovesicle and a targeted modified sound-sensitive agent nanovesicle; the sound-sensitive agent nanovesicle and targeted modified sound-sensitive agent nanovesicle have efficient and low-toxicity effects, a novel sound-sensitive agent material excellent in performance is provided for developing safe and efficient sonodynamic therapy, and by modifying surfaces of the sound-sensitive agent nanovesicle and the targeted modified sound-sensitive agent nanovesicle with specific antibodies or ligands, accumulation ofthe sound-sensitive agent nanovesicle and targeted modified sound-sensitive agent nanovesicle on a targeted portion is improved.

The invention relates to a preparation method and application of a dihydroporphine photosensitizer and a dihydroporphine sonosensitizer belonging to the chemical medicine field. The compounds have the general formula as shown in the specification, and the dihydroporphine photosensitizer and sonosensitizer compounds have different degrees of inhibition effects on human hepatoma cell line Hep G2 in in-vitro antineoplastic activity assessment. The ratios of optical activity to dark activity and sound activity to dark activity are higher than that of hematoporphyrin monomethyl ether as a positive control, and the method can be used for preparation of the photosensitizer and the sonosensitizer for photodynamic therapy sonodynamic therapy method for cancer treatment.

The invention discloses a multi-functional polypyrrole coated drug-loading mesoporous titanium dioxide nanoparticle and a preparation method thereof. Mesoporous titanium dioxide is used as a drug carrier and a sonosensitizer, combined with a good photothermal conversion material of polypyrrole, and capable of realizing the united anti-tumor effect of chemotherapy, photothermal therapy and sonodynamic therapy. A preparation technology for the polypyrrole coated drug-loading mesoporous titanium dioxide nanoparticle is convenient and simple. A particle size is about 156 nm. Passive targeting canbe realized through the high permeability and retention effect of a tumor site, the effective killing effect to tumor cells is realized, and the toxic and side effects to surrounding normal tissues and cells are reduced. In addition, the polypyrrole coated drug-loading mesoporous titanium dioxide nanoparticle also has a certain ultrasonic developing potency, and is capable of further realizing precision therapy on the tumor site. The polypyrrole coated drug-loading mesoporous titanium dioxide nanoparticle is good in biocompatibility, capable of reducing pain of a patient during a therapy period, and improving compliance of the patient, and has extensive application prospect.

The present discloses an ultrasonic adaptive focusing method for sonodynamic therapy. The method is employed to perform real-time regulation of focusing area of the sound energy and ultrasonic emission voltage when sonodynamic therapy is performed to accurately focus the sound energy onto a target area required by the treatment. The ultrasonic reflection echo is employed to perform real-time monitoring of a sound field state in an organization, and the focusing position, the focus size and the sound field intensity are regulated in real time according to a monitoring result to allow an actual sound field to be consistent to an expected sound field so as to solve the problem that there is deviation between the actual sound field and the expected sound field in the organization to cause bad treatment effect when the sonodynamic therapy is performed; and moreover, the reaction speed is fast, timeliness is good, manual intervention is little, the treatment efficiency and the stability of the treatment effect are improved, and therefore, the ultrasonic adaptive focusing method for sonodynamic therapy has high practicality and popularization values.

The invention discloses a lipidosome microbubble metal-loaded-ICG self-assembly composite system which has a multimode imaging / sonodynamic therapy (SDT) function. The system shows sonodynamic performance better than pure ICG molecules and has sonodynamic therapeutic effects of fixed-point explosion of lipidosome microbubbles, drug fixed-point release and increase of animal disease models of tumor,infection and atherosis. The invention further relates to sonodynamic therapy guided by metal ion / ICG@Microbubbless ultrasonic imaging, fluorescent imaging and photoacoustic imaging. The system is simple to prepare, cheap in price and remarkable in therapeutic effect.

The invention relates to a preparation method of a hollow metal oxide nano material. The preparation method comprises the following step: dispersing a prepared zeolite imidazolate framework ZIF-8 porous nano material which serves as a template into an ethanol-ethylene glycol mixed treatment solution to form a dispersion system; mixing a metal iron salt or manganese salt with ammonium chloride / ammonia water, or mixing the metal iron salt or manganese salt with an alkaline growth promoting liquid of sodium bicarbonate to form an iron or manganese ion complex precursor; mixing the precursor witha template solution, carrying out continuous stirring at room temperature for reacting, then centrifuging the product, and carrying out washing and vacuum-drying treatment to prepare a final sample. Under ultrasonic induction, hollow metal oxide nanoparticles show high active oxygen generation capacity, and have a great application value in tumor sonodynamic therapy. Compared with a traditional method, the method has the following obvious advantages: 1) the template is easy to prepare and is free of pollution; 2) synthesis regulation and control parameters are few, the process is simple, raw materials are low in cost, and industrial production can be achieved; and 3) the size of the product is controllable.

The invention discloses a composite sound-sensitive agent. The composite sound-sensitive agent comprises sound-sensitive agent nanoparticles and oxygen-producing microorganisms, under the irradiation of near-infrared laser, the oxygen-producing microorganisms continuously perform photosynthesis to produce oxygen, so that an anoxic tumor area is subjected to photooxygenation, and the sound-sensitive agent is ultrasonically excited to convert the oxygen into a large amount of singlet oxygen with cytotoxicity, so that tumor cells are effectively killed and tumor tissues are effectively damaged. Immunogen cell death caused by improvement of a tumor hypoxic environment and sonodynamic therapy is realized, and synergistic therapy of sonodynamic and immune is realized. Meanwhile, the composite sound-sensitive agent can be used for magnetic resonance imaging and computed tomography contrast imaging, and real-time monitoring of the sonodynamic therapy process is achieved. The work provides good theoretical and experimental support for development of good biocompatibility and effective sonodynamic force of hybrid microorganisms, and shows important clinical transformation prospects in sonodynamic force of microbial nano medicine.

The invention discloses metal-porphyrin nano-particles with sonodynamic therapy effects as well as a preparation method and the application thereof. Firstly, sulfonate ions and iron ions (Fe3<+>) of porphyrin are combined, and the metal-porphyrin nano-particles are formed by self-assembly. A nanostructure has more excellent sonodynamic therapy properties and molecular image monitoring and tracingproperties. In the invention, targeted peptide-modified Zn(II)-dimethylpyridamine dimer (Bis(DPA-Zn)-RGD) is prepared. Further, bis(DPA-Zn)-RGD with tumor targeting ability is modified on the surfaceby applying the ability to combine the sulfonate ions on the nano-TPPS surface with the Zn(II)-dimethylpyridamine (DPA-Zn). In combination with the surface-modified nanostructure and phosphate ions ofsiRNA, the siRNA can be loaded.

The invention relates to a far-field ultrasonic probe for sonodynamic therapy, and belongs to the technical field of medical instruments. The ultrasonic probe comprises an ultrasonic transducer, an upper ultrasonic transmission cavity, electric displacement shafts, a corrugated expansion piece, a lower ultrasonic transmission cavity, an ultrasonic sound absorption layer and an ultrasonic coupling layer, wherein one end of the upper ultrasonic transmission cavity is fixedly connected with the ultrasonic transducer, and the other end of the upper ultrasonic transmission cavity is connected with one end of the corrugated expansion piece; the other end of the corrugated expansion piece is connected with one end of the lower ultrasonic transmission cavity; the ultrasonic coupling layer is fixed at the other end of the lower ultrasonic transmission cavity; the electric displacement shafts are distributed in four directions around the corrugated expansion piece serving as a center; the inner surface of the lower ultrasonic transmission cavity is coated with the ultrasonic sound absorption layer. The ultrasonic probe has the characteristics of simple structure, easiness in implementation, safety to use, high efficiency, high persistence and the like.

The invention discloses a chlorins glucoside compound, and a preparation method and application thereof, and belongs to the technical field of chemical medicines. According to the chlorins glucoside compound disclosed by the invention, through the selective combination of the glucose transport protein which is highly expressed on the surface of the tumor cells, the targeting effect of a photosensitizer and a sound-sensitive agent on tumor cells is improved. The in-vitro anti-tumor activity evaluation shows that compared with chlorins e6 which is used as a control, the chlorins glucoside compound provided by the invention has relatively high light activity and sound activity on humanhepatoma carcinoma cells Hep G2. The chlorins glucoside compound disclosed by the invention can be used for preparation of a photosensitizer and a sound-sensitive agent in photodynamic therapy and sonodynamics therapy methods for tumor therapy. According to the invention, the preparation process is simple, the reaction conditions are easy to control, and the production is facilitated.

The invention discloses a novel composite nano preparation based on sonodynamic / immune combined therapy and a preparation method and application thereof, the novel composite nano preparation comprises a sound-sensitive agent, an immune activator, an active oxygen enhancing drug and liposome, the sound-sensitive agent and the active oxygen enhancing drug are entrapped in a liposome phospholipid bimolecular layer, and the immune activator is embedded on the lipidosome phospholipid bimolecular layer. The method comprises the following steps: (1) preparing a sound-sensitive agent, a cinnamyl aldehyde derivative, MPLA, a lecithin and DSPE-PEG5k into a solution; (2) evaporating out the solvent in the solution obtained in the step (1), and dispersing the drug-loaded lipid film in a buffer solution to obtain a drug-loaded liposome suspension; and (3) enabling the solution obtained in the step (2) to pass through a polycarbonate film to obtain the composite nano-liposome. The preparation can induce anti-tumor reaction, not only can prevent the development of in-situ solid tumors, but also can prevent the tumors from metastasis to far-end tissues. The composition is especially suitable for a combined treatment preparation of sonodynamic therapy and immunotherapy.

The present invention relates to compounds which are perylenequinone derivatives, their stereoisomers and atropisomers. These compounds can be particularly useful as photosensitizers or sononsensitizers in photodynamic or sonodynamic therapy. The invention also relates to various methods for using these compounds in photodynamic and / or sonodynamic therapy. The compounds also are useful as therapeutic agents for treating various hyperproliferative disorders.

The invention discloses an oxygen-carrying and drug-loading self-assembled nano-drug with molecular targeting / sonodynamic therapy (SDT) and a preparation method thereof. According to the nano-drug, erlotinib-modified chitosan (CE) is used as a carrier, then hematoporphyrin (HP) and perfluorooctane bromide (PFOB) are loaded, and CE / PFOB / HP self-assembled nanoparticles (CEPH) are prepared through self-assembly. As a sound sensitizer, the HP can play a role in SDT; and the PFOB carries oxygen and can improve the microenvironment of tumor relative to hypoxia, enhance the SDT effect and overcome the drug resistance of tumor cells to a chemotherapeutic drug Er (erlotinib) under the hypoxia condition. According to the nano-drug, molecular targeted therapy and SDT are combined, the PFOB is used for reversing drug resistance, and tumor cell proliferation can be inhibited to the maximum extent through cooperation of molecular targeted therapy, SDT and PFOB.

The invention belongs to the field of preparation of composite micelle entrapped drugs, and particularly relates to a composite nano micelle for multi-mode treatment of nasopharyngeal carcinoma and apreparation method and application thereof. The composite nano micelle for multi-mode treatment of nasopharyngeal carcinoma is characterized by comprising Bangladesh rose red and an amphiphilic peptide nano micelle for encapsulating the Bangladesh rose red. According to the invention, the amphiphilic peptide of C18-GRRRRRRRRGDScontaining a tripeptide sequence of arginine, glycine and aspartic is self-assembled into a nano micelle, and the inside of the nano micelle is wrapped with Bangladesh red; the results show that the a combined therapy group has obvious inhibitory effect on CNE-2Z tumor during intravenous injection. During intratumor injection, a photodynamic therapy group (RBNs+Laser) with laser added alone, a sonodynamic therapy group (RBNs+US) with ultrasound added alone and a combination therapy group (RBNs+Laser+US) all have a significant inhibitory effect on tumor growth.

The invention discloses a preparation method of copper porphyrin-folate liposome nanoparticles and application thereof as a sound-sensitive agent. The preparation method comprises the following steps:dissolving copper porphyrin with methanol, dissolving lecithin and folate liposome in chloroform, mixing the two solutions, preparing a lipid film by a rotary evaporation method, and carrying out ultrasonic hydration with ultrapure water to synthesize the copper porphyrin-folate liposome nanoparticles. The nanoparticles have excellent targeting property in tumor cells with high expression of folate receptors, and are beneficial to enrichment at tumor sites so as to improve the anti-tumor effect; and under ultrasonic excitation, the copper porphyrin absorbs sound energy to generate transitionand converts surrounding oxygen into singlet oxygen to kill tumor cells. The invention provides the sound-sensitive agent capable of generating singlet oxygen through ultrasonic excitation to kill thetumor cells, and the folate targeted liposome is used as a carrier to carry the sound-sensitive agent, so that the water solubility and the targeting property are improved, and the sonodynamic therapy (SDT) effect is further improved.

The present disclosure provides methods for treating diseased cells in a subject using sonodynamic therapy (SDT), comprising: administering to the subject a sonosensitizer composition comprising IRDye® 700DX, wherein the sonosensitizer composition associates with the diseased cell; and thereafter applying an ultrasonic wave to the diseased cell.

The invention discloses hydroxyl acetylated curcumin and application thereof in preparation of an ultrasonic sensitive agent for treating atherosclerosis. The hydroxyl acetylated curcumin is a novel drug which is synthesized by carrying out acetylation modification on an unstable group, namely hydroxy of curcumin. Compared with curcumin, hydroxyl acetylated curcumin is more stable in chemical property. Hydroxyl acetylated curcumin-mediated sonodynamic therapy mainly comprises the following steps that intracellular hydroxyl acetylated curcumin is ultrasonically activated to generate singlet oxygen, the singlet oxygen acts on mitochondria to open permeability transition pores (PTP) of the mitochondria, mitochondrial outer membrane potential is reduced and the outer membrane is cracked, substances such as cytochrome C, reactive oxygen species (ROS) and the like inside the mitochondria enter cytoplasm to activate a Caspase passage, intranuclear chromatin is destroyed, and finally cell apoptosis is caused. An experiment result shows that the THP-1 macrophage apoptosis can be effectively induced by the hydroxyl acetylated curcumin-mediated sonodynamic therapy, and the cell survival rate is reduced.

The present invention provides a metal-ICG complex, a preparation method of the complex, metal-ICG complex albumin nanoparticles and a preparation method and application of the nanoparticles, and relates to the technical field of nano-drugs. Metal ions of the metal-ICG complex are selected from at least one of gold, platinum, zinc, copper, cobalt, iron, nickel and manganese. The technical problemsof few types, short maximum illumination wavelength and narrow therapeutic window of an existing acoustic sensitive agent are improved; and the metal ions and ICG in the metal-ICG complex provided bythe invention are complexed by coordination bonds, so that the type of acoustic sensitive agents is expanded, the illumination wavelength of the acoustic sensitive agents is increased, the therapeutic window is extended, and the metal-ICG complex has double functions of photosensitivity and acoustic sensitivity and can effectively promote the development of photothermal and sonodynamic therapy.