104 results about "Sonodynamic therapy" patented technology

Disclosed herein are activatable inactive sonosensitizer compounds, referred to as locked sonosensitizer compounds, designed with a detachable stimuli-responsive tether acting as a trigger or safety, as these compounds restore their inherent sonosensitization activity upon the detachment of the tether. The invention further encompasses pharmaceutical compositions comprising the same, offering a strategic approach to sonodynamic therapy, whereas the compounds and compositions are formulated to be administered and applied under favorable conditions, enhancing their efficacy in the context of therapeutic applications, since the therapeutic methods presented herein are more versatile and efficient compared to presently known SDT methods due to the controlled activation of the sonosensitizer.

The invention belongs to the technical field of medical nano-materials, and particularly relates to a lactoferrin nano-material for sonodynamic regulation and control of blood-brain barrier permeability as well as a preparation method and application of the lactoferrin nano-material. The nanomaterial is nanoparticles LCe6 formed by self-assembly of lactoferrin and chlorin e6, can target brain microvascular endothelial cells, and can temporarily, reversibly and targeting open BBB by using the sonodynamic effect of the LCe6 in combination with transcranial ultrasonic stimulation, so that the accumulation of the nanoparticles LCe6 at a focus part is improved; in addition, the nanoparticle LCe6 also has a targeting property on brain tumor cells, the tumor cells can be accurately killed by utilizing the sonodynamic effect of the nanoparticle under secondary transcranial ultrasound, and the effect of sonodynamic treatment on brain glioma is exerted to the maximum extent; the nanoparticles LCe6 have excellent biocompatibility and low toxicity; according to the invention, the intracerebral delivery efficiency of the drug can be obviously improved, and a novel method for targeting open brain disease BBB is provided.

The invention discloses an ultrasonic controllable multifunctional microneedle patch as well as a preparation method and application thereof, and belongs to the technical field of biological materials. The preparation method comprises the following steps: preparing crystalline CoW-LDH nanosheets from Co (NO3) 2.6 H2O, Na2WO4. 2H2O and NaOH, dispersing the crystalline CoW-LDH nanosheets in PBS (Phosphate Buffer Solution), centrifuging and collecting the crystalline CoW-LDH nanosheets, and re-dispersing the crystalline CoW-LDH nanosheets in ultrapure water to obtain amorphous CoW-LDH nanosheets; adding PEG (Polyethylene Glycol), ultrasonically dispersing, stirring at room temperature, centrifuging, collecting and centrifugally washing with ultrapure water to obtain a PEG modified amorphous CoW-LDH nanosheet; and adding microalgae and an amorphous CoW-LDH nanosheet modified by PEG (Polyethylene Glycol) to prepare the ultrasonic controllable multifunctional microneedle patch. The multifunctional microneedle patch disclosed by the invention is high in biological safety, can realize ultrasonic controllability, has a sonodynamic treatment effect and an oxygen supply effect, remarkably inhibits melanoma recurrence and remarkably promotes wound healing, and has a wide application prospect.

The invention discloses an aptamer-modified lipid nano delivery platform as well as a preparation method and application thereof, and belongs to the field of biomedicine. The platform is prepared by taking DPPC, DOTAP and cholesterol as basic lipid components, Ce6 as a sound-sensitive agent and Flt3L as an immune agonist, controlling the particle size through gradient extrusion, and coupling cholesterol with an EpCAM aptamer for surface modification. The method has the core advantages that active targeting enrichment of tumors is realized by virtue of the aptamer, tumor cell immunogen cell death (ICD) is induced in combination with a sonodynamic therapy (SDT), and damage-related molecular patterns (DAMPs) are released; meanwhile, Flt3L is released in a tumor microenvironment, collection and activation of type 1 classical dendritic cells (cDC1) are specifically promoted, an'endogenous cDC1 vaccine 'is constructed, and CD8 + T cell mediated anti-tumor immune response is enhanced. Experiments prove that the platform can significantly improve the cDC1 infiltration level of a tumor site, effectively inhibit the progress of prostate cancer (PCa), and provide a new normal form for immune'cold tumor 'treatment.

The invention discloses an ionic iridium complex sonosensitizer as well as a preparation method and application thereof, belongs to the technical field of metal complex preparation, provides four ionic iridium complex sonosensitizers with excellent treatment effects, and provides a preparation method of the ionic iridium complex and application of the ionic iridium complex sonosensitizers in tumor sonodynamic therapy. The ionic iridium complex sonosensitizer prepared by the invention shows remarkable affinity to mitochondria, and due to more negative potentials in cancer cells, the cationic iridium complex targeting cancer cells is more favorably realized, and meanwhile, due to the introduced triphenylamine structure, the AIE behavior of the triphenylamine structure is realized, so that the targeting effect of the cationic iridium complex on the cancer cells is improved. The ionic iridium complex sonosensitizer can activate the sonosensitizer in an aggregation state to promote the generation of ROS, the triplet excited state energy level of the ionic iridium complex sonosensitizer can be well matched with the excited state energy level of oxygen, and meanwhile, the ionic iridium complex sonosensitizer has a relatively long triplet excited state life, so that the ROS generation capability of the ionic iridium complex sonosensitizer is relatively high; and the material is a candidate material of a sound-sensitive agent with an excellent sonodynamic therapy effect.

The invention discloses a composite nano sound-sensitive agent as well as a preparation method and application thereof. According to the composite nano sound-sensitive agent provided by the invention, a conjugated system of the narrow-gap semiconductor nano sound-sensitive agent core / the ferric oxide nano functional layer / the surface targeting antibody is constructed, so that multi-mode synergy integrating sonodynamic therapy, ferroptosis induction and immunoregulation is realized. The composite nano sonosensitizer further has precise targeting, efficient sonodynamic effect, ferroptosis enhancement effect and good biocompatibility, a targeting antibody on the surface of the composite nano sonosensitizer can specifically recognize high-expression antigens on the surfaces of tumor cells, and dual-targeting enrichment of a treatment system is achieved in combination with magnetic responsiveness guidance; the coating of the iron oxide nanoparticles obviously enhances the sound-sensitive performance of the inner core of the sound-sensitive agent, improves the ROS generation efficiency, and overcomes the hypoxia limitation of the tumor microenvironment; iron oxide is used as an iron source to continuously release Fe < 2 + >, and cooperates with ROS generated by sonodynamic therapy to induce ferroptosis, so that a ferroptosis enhancing effect is generated.

The application belongs to the technical field of medicine, and particularly relates to a La2(WO4)3 / CuWO4 composite sonosensitizer, a preparation method thereof and application thereof in sonodynamic therapy. The La2(WO4)3 / CuWO4 composite sonosensitizer contains 5-40% of La2(WO4)3 in terms of mass percentage. The application takes U251 glioma cells as a model target, and explores the application of the La2(WO4)3 / CuWO4 composite sonosensitizer in sonodynamic therapy of tumors. The results show that the La2(WO4)3 / CuWO4 composite sonosensitizer has a good sonodynamic therapy effect on U251 glioma cell lines after 1MHz US irradiation for 1min.

The present invention provides a glutathione-activated co-assembled nanoprobe and a preparation method and application thereof, belonging to the technical fields of chemical synthesis, biological analysis and detection, tumor imaging and treatment. In the present invention, amphiphilic small molecule probes 1-Zn-PPA and 1-NLG are constructed, and the probes 1-Zn-PPA and 1-NLG can be co-assembled in a solution to form co-assembled nanoprobes 1-NPs. The nanoprobes 1-NPs can accumulate at the tumor site under the tumor EPR effect and the targeting action of cRGD on the probe surface, and disassemble under the action of GSH present in the tumor reducing environment to release Gd-containing hydrophilic small molecule 2-Gd, light / sound-sensitive drug Zn-PPA-SH and immune adjuvant drug NLG919. The nanoprobes 1-NPs can realize combined sonodynamic therapy / photodynamic therapy / immunotherapy of living tumors. The nanoprobes 1-NPs have good applications in fluorescence imaging, magnetic resonance imaging, and the preparation of tumor treatment drugs and tumor-killing devices.

The present invention discloses an ultrasound-responsive CO-releasing nanoformulation and a preparation method thereof. First, a vanadium tetrasulfide nanoformulation with sonodynamic and chemodynamic effects is prepared. Then, a CO-releasing molecule is loaded to construct an ultrasound-responsive CO-releasing nanoformulation, realizing the controllable release of CO. The preparation process of the ultrasound-responsive CO-releasing nanoformulation is simple, and it can achieve the combination of CO therapy / sonodynamic therapy / chemodynamic therapy for tumors. Using it for tumor treatment has safety and effectiveness, and has broad application prospects.

The present disclosure relates generally to methods of utilizing sonosensitizers in the presence of thiamine or a derivative thereof for the purpose of cross-linking animal tissue, producing singlet oxygen within or at the site of animal tissue, or treating a condition in a subject, wherein the condition is an aneurysm, cancer, and / or a scleral condition.

The present invention belongs to the technical field of sonodynamic therapy, and discloses a two-dimensional TiO2 nanomaterial with peroxidase activity and its application in sonodynamic therapy. The present invention firstly proposes to design ultrathin 2D TiO2 nanosheets with peroxidase activity as efficient sonosensitizers for sonodynamic cancer therapy. The ultrathin Ti3C2 nanosheets obtained by acid etching treatment are oxidized to 2D TiO2 through simple heat treatment. Surprisingly, 2D TiO2 has peroxidase activity, and when used as a sonosensitizer, the 2D TiO2 nanosheets exhibit excellent ROS generation activity under US irradiation. In vitro experiments show that the 2D TiO2 nanosheets can effectively induce apoptosis of cells under US irradiation.

The application discloses a kind of sustainable oxygen production sonodynamic therapy nanoparticles, including core-shell structure, the core of core-shell structure is located in shell inside, the core of core-shell structure is manganese oxide nanoparticle, the shell of core-shell structure is the high molecular polymer of carrying sound sensitizer, manganese dioxide nanoparticle is manganese dioxide nanoparticle, sound sensitizer is one of porphyrin and its derivatives, high molecular polymer is polylactic acid glycolic acid copolymer, this kind of sustainable oxygen production sonodynamic therapy nanoparticles by using high molecular polymer simultaneously carrying manganese dioxide nanoparticle and sound sensitizer to enhance SDT effect, high molecular polymer has good biocompatibility and drug controlled release performance, not only can reduce the toxicity of manganese dioxide to normal tissue, can also make it continuously controllable with tumor site excess H2O2 Reaction generates oxygen, reverses tumor hypoxic microenvironment while providing sufficient oxygen for SDT, under the action of ultrasound, generate a large amount of ROS to inhibit tumor.

The invention relates to a substance with a Prussian blue structure, which can generate hydroxyl radicals, superoxide anions and singlet oxygen in parallel under ultrasonic irradiation, and in the presence of ultrasonic irradiation and H2O2, the substance with the Prussian blue is used as a sonodynamic therapy (SDT) means to kill focus cells by generating sonotoxic and cytotoxic free radicals, so as to achieve the purpose of improving the therapeutic effect of the substance with the Prussian blue structure. Therefore, diseases are effectively treated.

A porphyrin compound for bioimaging and sonodynamic therapy, as well as its preparation method and application, has a chemical formula shown in Formula 2. The preparation method comprises the following steps: adding p-methylthiobenzaldehyde, a compound of Formula 1, and pyrrole to a reaction flask, evacuating the flask and introducing nitrogen gas, followed by adding anhydrous dichloromethane to obtain a reaction solution; bubbling nitrogen gas through the reaction solution, stirring at 25°C, adding boron trifluoride etherate, maintaining the temperature while continuing to stir, adding DDQ, continuing stirring, adding triethylamine, filtering, concentrating, and purifying to obtain the target product. This method features mild reaction conditions, a short reaction time, and simple operation. The resulting porphyrin compound exhibits excellent sonosensitivity and biocompatibility, can efficiently generate reactive oxygen species under ultrasound, and can produce excellent sonodynamic effects. The compound also exhibits fluorescence emission in the near-infrared region and can be excited by visible light, enabling confocal imaging to detect changes in cell morphology before and after, resulting in excellent bioimaging results. #imgabs0#

The present invention relates to the technical field of pharmaceutical chemistry, and particularly relates to a zinc complex and its preparation method and application. The chemical structural formula of the zinc complex is As a sonosensitizer, it has the advantages of good biosafety, clear structure, and easy metabolism. Under the condition without ultrasound, its killing ability to cells is extremely small and the biosafety is good; under the condition of ultrasound irradiation, it can generate a large amount of singlet oxygen <supgt;1< / supgt;O<subgt;2< / subgt>, thereby destroying the intracellular redox balance, resulting in the death of cancer cells. Its IC50 value for 4T1 cells can reach 2.45 μM, showing excellent anti-tumor effects and is expected to be used in the sonodynamic therapy of tumors.

The invention belongs to the field of biological materials, and particularly discloses a multi-component microcapsule with multiple ultrasonic responses as well as a preparation method and application of the multi-component microcapsule with the multiple ultrasonic responses, and a hydrogel microcapsule with structural and functional design is prepared through a microfluidic double-emulsion technology on the basis of light-cured methacrylic anhydride gelatin hydrogel and liquid decafluoropentane. According to the prepared multi-ultrasonic-responsive multi-component microcapsule, ultrasonic-responsive oxygen release and controllable generation of reactive oxygen free radicals are realized on the basis of curcumin loaded in methacrylic anhydride gelatin and oxygen dissolved in decafluoropentane; the released oxygen provides an oxygen raw material for sonodynamic therapy while relieving a hypoxic environment in an articular cavity, so that generation of reactive oxygen free radicals is enhanced, apoptosis of pathological synovial fibroblasts is induced, and synovial proliferation in the pathological articular cavity is inhibited.

The invention relates to a preparation method of a PEDOT-based nano composite material. The preparation method comprises the following steps: S10, preparing PEDOT nano particles; s20, preparation of the PEDOT / AB nano particles; and S30: preparing the PEDOT / AB (at) CMs nano particles, wherein the obtained material is the PEDOT-based nano composite material. According to the preparation method of the PEDOT-based nano composite material and the material, a homologous cancer cell membrane is introduced to construct a gas-wrapped vesicle structure, the immunological rejection can be reduced while the ultrasonic imaging effect is enhanced, and ultrasonic imaging and sonodynamic therapy are combined, so that the tumor detection precision is improved, and the detection sensitivity is improved. And precise targeted therapy can also be realized.

The application belongs to the technical field of biomedical materials, and particularly relates to a supramolecular coordination complex with sonodynamic activity and a preparation method and application thereof. The application proposes a new supramolecular coordination complex formed by self-assembly of a phthalocyanine compound and Ru-dbq, and finds that the supramolecular coordination complex has excellent ROS yield in response to US, and also has the characteristics of multifunctional integration such as CAT enzyme-like activity and antioxidant activity, which endows the material with great application potential in response to complex biological environments and excellent sonodynamic therapy prospects.

The invention discloses a responsive nano-drug controlled release system based on sonodynamic therapy and application thereof, and belongs to the technical field of tumor immunotherapy, the responsive nano-drug controlled release system based on sonodynamic therapy comprises barium titanate nanoparticles; the hydrogel responds to active oxygen, and the hydrogel is connected through active oxygen sensitive bonds and fractures under the action of ROS (reactive oxygen species), so that wrapped barium titanate nanoparticles are released. As a further optimization scheme of the invention, the particle size of the barium titanate nano-particles is 10-100nm, and the barium titanate nano-particles are cubic. As a further optimization scheme of the invention, the hydrogel is composed of TPA and PVA. The tumor treatment responsive nano-drug provided by the invention shows huge potential in the field of tumor immunotherapy, provides theoretical and experimental basis for design and application of a nano-drug controlled release system in the future, and is expected to bring new breakthrough to tumor treatment.

The present invention relates to an enhanced sonodynamic therapeutic agent using a liposome having the ability to transfer oxygen, and more particularly, to lipid nanoparticles having the ability to transfer oxygen in which perfluorocarbon (PFC) and indocyanine green (ICG) are loaded inside liposomes to increase the efficiency of sonodynamic therapy.

The invention belongs to the field of biological materials, and particularly relates to an ultrasonic responsive oxygen-carrying and traditional Chinese medicine microcapsule and a preparation method and application thereof in treatment of infected wounds, two glass tubes with different inner diameters are coaxially sleeved and fixed, the glass tube with the larger inner diameter is used as an outer phase channel, and the glass tube with the smaller inner diameter is used as an inner phase channel; the preparation method comprises the following steps: dissolving sodium alginate and curcumin into deionized water to prepare a mixed pre-gel solution; oxygen is introduced into the liquid fluorocarbon, and oxygen-enriched fluorocarbon liquid is obtained after treatment; the prepared mixed pre-gel solution and the oxygen-enriched fluorocarbon liquid are simultaneously introduced into the external phase channel and the internal phase channel respectively, electric field force is applied to outlets of the channels, and the mixed pre-gel solution and the oxygen-enriched fluorocarbon liquid fall into a collection liquid after being subjected to electronic injection to obtain the oxygen-carrying and traditional Chinese medicine microcapsule. The loaded traditional Chinese medicine curcumin and oxygen dissolved in the fluorocarbon achieve simultaneous delivery of traditional Chinese medicine and oxygen, and controllable oxygen release and sonodynamic therapy can be achieved.

The invention provides a sound-sensitive-agent-loaded targeted adipose tissue extracellular vesicle for assisting sonodynamic treatment of obesity as well as a preparation method and application of the sound-sensitive-agent-loaded targeted adipose tissue extracellular vesicle, and particularly belongs to the technical field of obesity drug preparation. The invention provides an application of an extracellular vesicle of a targeted adipose tissue loaded with a sound-sensitive agent in preparation of a medicine for assisting sonodynamic treatment of obesity. The sound sensitive agent is hematoporphyrin monomethyl ether; the extracellular vesicle of the targeted adipose tissue is an extracellular vesicle of which the surface expresses targeted adipose tissue sequence peptide ATS, and the amino acid sequence of the ATS is as shown in SEQ ID NO. 1. The extracellular vesicle loaded with the haematoporphyrin monomethyl ether surface expression targeting adipose tissue sequence peptide ATS can accurately induce adipose tissue browning, finally safe and effective fat reduction is achieved, and the effect of treating obesity is achieved.

The invention discloses a tumor treatment synergistic interaction system and method based on the acoustic cavitation effect of a phase-change nano-droplet. The system comprises the phase-change nano-droplet entrapped with a chemotherapeutic drug and a sound-sensitive agent and a cavitation effect intelligent monitoring and adjusting system. The method is characterized in that cavitation noise signals of a tumor target area are collected in real time through a cavitation monitoring system integrated in an ultrasonic probe, the inertial cavitation dosage is rapidly calculated through a signal processing system and compared with a preset threshold value, then a feedback instruction is generated to dynamically regulate and control ultrasonic irradiation parameters, and intelligent closed-loop control is formed. According to the method, the cavitation effect can be accurately maintained in an ideal treatment window, targeted release and deep penetration of the medicine and activation of the sound-sensitive agent are synchronously achieved through cavitation mechanical energy, and time-space synchronous synergistic interaction of chemotherapy and sonodynamic therapy is achieved.

The present invention belongs to the field of biomedicine, and specifically relates to a diagnostic and therapeutic integrated probe responsive to carboxylesterase, and a preparation method and application thereof. The present invention discloses a small molecule diagnostic and therapeutic probe represented by the following structural general formula. This small molecule diagnostic and therapeutic probe is obtained by reacting a near-infrared fluorescent dye, chlorambucil, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC), and 4-dimethylaminopyridine (DMAP) in an organic solvent. The present invention encapsulates the above small molecule diagnostic and therapeutic probe with poly(lactic acid-co-glycolic acid)-polyethylene glycol (PLGA-PEG) to obtain a nano diagnostic and therapeutic integrated probe. The fluorescence wavelength of the obtained nano diagnostic and therapeutic integrated probe is located in the near-infrared region, which can realize near-infrared fluorescence imaging diagnosis of tumors, and has combined therapeutic properties of chemotherapy, photodynamic therapy, and sonodynamic therapy, and can significantly kill tumor cells.

The present invention relates to the field of medical technology, and particularly relates to an aggregation-induced emission photosensitizer and its application for treating in-situ breast cancer by sonodynamic therapy. The preparation method of the photosensitizer includes: taking a TTh derivative or a pharmaceutically acceptable salt of the TTh derivative, and adding the tetrahydrofuran solution of PAE-PEG to ultrapure water to obtain a first solution, and the chemical formula of the TTh derivative is: ; stirring the first solution, and then performing ultrafiltration to obtain nanoparticles; dispersing the nanoparticles in a buffer solution to obtain the photosensitizer. The photosensitizer of the present invention can effectively overcome the π-π stacking effect, maintain a certain free radical yield in the aggregated state, and after being modified by PAE-PEG, can target tumor regions with an acidic microenvironment, increase the uptake of tumor cells, reduce the impact on normal tissues, and reduce side effects.

Ultrasound transducer element arrays using acoustic ensonification drive patterns via a patient interface for sonosensitizer activation in sonodynamic therapy. Incoherent acoustic field generation varying phase, frequencies, and / or amplitude via controlled delivery of low intensity planar acoustic waves. Method includes generating a first and a second signal to generate respective acoustic ensonification drive patterns with phase, frequency, and amplitude and generating at least one relative phase, frequency or amplitude difference to generate a third incoherent acoustic ensonification pattern to activate a sonosensitizer. Calibration and complementary therapy procedures to improve cell susceptibility of cells to sonodynamic therapy are disclosed.

The invention relates to a preparation method of a conjugated microporous polymer type sonosensitizer and application of the conjugated microporous polymer type sonosensitizer in sonodynamic therapy (SDT), and belongs to the crossing field of organic optoelectronic materials and biomedicine. A conjugated microporous polymer with triphenylamine (TPA) as a main body and connecting molecules with different lengths is designed. A series of conjugated microporous polymer sonosensitizers with different pores are constructed by taking triphenylamine with a trident structure as a main body and connecting monomers with different lengths, and have a good sonodynamic treatment effect.

The invention relates to the technical field of research and development of tumor photodynamic therapy photosensitizers, photothermal therapy photothermal reagents and sonodynamic therapy sonosensitizers, and provides a preparation method and application of a binuclear metal complex of a polythiophene bridging ligand. The invention relates to a ruthenium complex TBLRu3a-TBLRu6a and a ruthenium complex TBLRu3b-TBLRu6b of a polythiophene bridged tri-bidentate ligand, a ruthenium complex TTLRu1-TTLRu12 of a polythiophene bridged bis-tridentate ligand, and an osmium complex TTLOs1-TTLOs6 of a polythiophene bridged bis-tridentate ligand, which are respectively designed and invented by the invention. The invention further relates to a preparation method of the ruthenium complex TBLRu3a-TBLRu6a and the ruthenium complex TBLRu3b-TBLRu6b of the polythiophene bridged tri-bidentate ligand and a preparation method of the ruthenium complex TTLRu1-TTLRu12 of the polythiophene bridged bis-tridentate ligand. The polythiophene bridged metal complex has extremely high stability and excellent two-photon absorption characteristic, has excellent biotoxicity and photothermal conversion capability under the action of infrared exciting light, and can induce A549R cell apoptosis. The polythiophene bridged metal complex provided by the invention is a potential novel two-photon absorption photodynamic therapy photosensitizer, a photothermal therapy photothermal reagent and a sonodynamic therapy sonosensitizer, and has a wide application prospect in photodynamic therapy, photothermal therapy and sonodynamic therapy of tumors.

The invention discloses efficient tumor sonodynamic therapy nanoparticles based on carbon-doped nano-zinc oxide as well as a preparation method and application of the efficient tumor sonodynamic therapy nanoparticles. Belongs to the field of biomedicine. On the basis of MOF-5, carbon-doped hollow-hole-shaped nano-particles C-ZnO are formed after calcination in a nitrogen atmosphere, then chloroplatinic acid hexahydrate and sodium borohydride are added into a C-ZnO nano-particle solution, so that Pt nano-particles are loaded on the surface of C-ZnO, in addition, AB is loaded in a CZP hollow structure, HA-AA modification is carried out on the surface of the CZP hollow structure, and the CZP hollow structure loaded with the AB is obtained. Therefore, the effects of hydrogen treatment, consumption of excessive glutathione in a biological microenvironment and targeting are achieved, and the effect of treating cancers is achieved from multiple aspects. The nano particles are uniform in size, the average particle size is about 200 nm, good biocompatibility is achieved, and the material prepared through the method has excellent ROS generation capacity, so that the material has excellent sonodynamic therapy potential and has wide application prospects in the field of tumor therapy.

This invention discloses a FA-ICG-NBs nanobubble, its preparation method, and its application in the preparation of tumor-targeted therapeutic agents. The FA-ICG-NBs nanobubble employs a composite phospholipid shell, with folic acid (FA) covalently bonded to the surface and indocyanine green (ICG) loaded onto it, encapsulating a mixture of oxygen and sulfur hexafluoride. The preparation method includes: preparing a lipid solution for forming the composite phospholipid shell; mixing the lipid solution with the oxygen and sulfur hexafluoride mixture, and then performing a reciprocating shearing gas-liquid interface self-assembly to obtain the FA-ICG-NBs nanobubble. The FA-ICG-NBs nanobubble prepared by this invention utilizes dual-wavelength photoacoustic imaging for non-invasive assessment of tumor blood oxygenation and hypoxia improvement, combined with ICG fluorescence timing for screening the optimal therapeutic window, and synergistic oxygen-enhanced sonodynamic therapy. It exhibits good biocompatibility, structural stability, and integrates targeted enrichment, blood oxygenation assessment, hypoxia monitoring, timing regulation, and enhanced therapeutic effects.