391results about "Disrupted materials" patented technology

The present invention relates to an immunomicrobubble complex and its uses. The immunomicrobubble complex (IMC) according to the present invention comprises microbubbles conjugated with an antibody, wherein the microbubbles have excellent stability and excellent antibody binding strength, and it has been confirmed that when the immunomicrobubble complex is treated with high-intensity focused ultrasound (HIFU), the anti-tumor effect is significantly enhanced and an immune-enhancing effect is exhibited. Therefore, the immunomicrobubble complex according to the present invention is expected to improve the delivery efficiency of the conjugated antibody and can be used for the diagnosis and treatment of cancer, and exhibits various functions in the field of immunotherapy, including a contrast effect, improvement of half-life, improvement of drug delivery, lymphocyte concentrating effect, cancer immunotherapy, and ultrasound-induced immunotherapy.

The invention belongs to the technical field of biomedical materials, and relates to ultrasonic response adhesion integrated hydrogel as well as a preparation method and application thereof, the hydrogel comprises a hydrogel matrix, an interface anchoring agent and a functional load component; the preparation method comprises the following steps: 1, preparing a hydrogel precursor solution, and preparing a hydrogel matrix into a solution; 2, carrying out mold molding or injection crosslinking on the solution prepared in the step 1 to form hydrogel; 3, preparing an interface anchoring agent solution, and dissolving or dispersing an interface anchoring agent in a solvent to prepare the interface anchoring agent solution; the ultrasonic responsive adhesive hydrogel platform provided by the invention can provide an efficient, controllable and universal solution for tissue adhesion, targeted therapy, wearable attachment and the like in a complex microenvironment, and is suitable for a plurality of clinical scenes such as chronic wound management, surgical postoperative closure, infection control, adhesive bioelectronic fixation, soft tissue engineering and the like.

The present invention relates to sonosensitive liposomes and a method for preparing the same. The sonosensitive liposomes according to the present invention improve the delivery efficiency of encapsulated drugs, and are expected to exhibit various functions in the fields of cancer treatment and drug delivery carriers by improving drug delivery effects through targeted cancer cells when used in combination with ultrasonic treatment.

Hydrogen-bonded framework nanoparticles comprising a chemiluminescent compound (e.g., L-012) are provided and can be used, e.g., for sono-optogenetic treatment of neurodegenerative diseases such as Parkinson's disease. Related in vivo and therapeutic methods are also provided.

The present invention relates to ultrasound (US) mediated delivery of therapeutic agents, such as the delivery of a drug, gene, nanoparticle or radioisotope, using a bi-phasic microparticle system comprising gas microbubbles, emulsion microdroplets and clusters thereof. Thus, the present invention relates to a cluster composition and a pharmaceutical composition, and their use for delivery of therapeutic agents and as a contrast agent for ultrasound imaging. It further relates to methods for delivering such therapeutic agents and to the use of said compositions.

The invention provides an ultrasonic activated blood coagulation targeting tumor selective prodrug as well as a preparation method and application thereof. The blood coagulation targeting tumor selective prodrug has a structure as shown in a formula I. The blood coagulation targeting tumor selective prodrug can trigger a blood coagulation cascade reaction by utilizing tumor vascular injury generated by ultrasonic induction so as to realize targeted anchoring of the drug and inhibit off-target diffusion of an active drug; meanwhile, the prodrug is activated by ultrasound, so that the limitation of tumor heterogeneity on the activation of the prodrug can be overcome. According to the technical scheme, selective enrichment of active drugs in tumors can be effectively improved, and toxicity caused by drug off-target is reduced while tumor growth is remarkably inhibited.

The present disclosure related to a method of using high frequency sound waves directed toward an acoustically active particle. The high frequency sound waves may be configured to enlarge the acoustically active particle and oscillate the acoustically active particle, thereby facilitating extravasation of an agent to a tissue compartment of a region of interest.

The invention belongs to the technical field of biological medicines, and particularly discloses a nano-engineering T cell membrane coated nano-particle as well as a preparation method and application of the nano-engineering T cell membrane coated nano-particle. Nanoparticles of a specific structure are constructed based on a phospholipid bilayer bionic nanometer platform through a membrane fusion technology, immune clearance caused by phagocytosis of the nanoparticles by macrophages can be avoided through disguise of a T cell membrane, and the blood circulation time is prolonged; the functions of T cells are recovered through specific blocking of immune checkpoint molecules loaded on the immune checkpoint molecules on corresponding immune checkpoint ligands on tumor cells; under ultrasonic radiation, the nano-particles are gradually decomposed and release the sound-sensitive agent to generate a large amount of active oxygen, so that immunogenic death of tumor cells is promoted, more cytotoxic T lymphocytes are recruited to infiltrate into tumor parts, and the recovery of T cell mediated immune response function is facilitated. The synergistic effect of the sound-sensitive agent and the immune checkpoint protein can effectively inhibit tumor growth, induce a long-term immune memory effect and prevent tumor metastasis and recurrence.

Theranostic targeted ultrasonic blasting nano-bubbles, a preparation method therefor, and use thereof, wherein the nano-bubbles are the targeted drug-gene nano-bubbles miRNA / FTY720 / PFP@PEI-T7 NBs. The nano-bubbles can be used for preparing a thyroid ultrasound diagnostic agent or a drug for treating thyroid cancer.

Disclosed herein is a composition for enhanced ultrasound ablation and methods relating to same. The composition may include a microbubble-microdroplet cluster composition, wherein the microbubble-microdroplet cluster composition may include groups of negatively charged microbubbles and positively charged microdroplets permanently held together by their opposing electrostatic attractive forces to form a single, agglomerated entity.

The invention belongs to the technical field of medical nano-materials, and particularly relates to a lactoferrin nano-material for sonodynamic regulation and control of blood-brain barrier permeability as well as a preparation method and application of the lactoferrin nano-material. The nanomaterial is nanoparticles LCe6 formed by self-assembly of lactoferrin and chlorin e6, can target brain microvascular endothelial cells, and can temporarily, reversibly and targeting open BBB by using the sonodynamic effect of the LCe6 in combination with transcranial ultrasonic stimulation, so that the accumulation of the nanoparticles LCe6 at a focus part is improved; in addition, the nanoparticle LCe6 also has a targeting property on brain tumor cells, the tumor cells can be accurately killed by utilizing the sonodynamic effect of the nanoparticle under secondary transcranial ultrasound, and the effect of sonodynamic treatment on brain glioma is exerted to the maximum extent; the nanoparticles LCe6 have excellent biocompatibility and low toxicity; according to the invention, the intracerebral delivery efficiency of the drug can be obviously improved, and a novel method for targeting open brain disease BBB is provided.

The invention belongs to the technical field of biological medicines, and particularly discloses a bionic nano-vesicle for regulating and controlling neutrophil phenotype in combination with ultrasound, and a preparation method and application of the bionic nano-vesicle. The bionic nano-vesicle provided by the invention can be broken under the action of high-concentration glutathione existing in a tumor tissue microenvironment to release the sound-sensitive agent and functional siRNA, and under the action of US irradiation, the sound-sensitive agent is activated to generate a large amount of ROS, promote tumor cell DNA breakage, generate a large amount of double-stranded DNA, activate APC and promote IFN-beta release, so that the tumor tissue microenvironment is inhibited. The released functional siRNA can promote the N2-type neutrophile granulocyte to become N1-type neutrophile granulocyte, and the released functional siRNA can prevent the N1-type neutrophile granulocyte from being reversed to the N2-type neutrophile granulocyte again, so that the regulation and control on the phenotype of the neutrophile granulocyte are realized; under catalysis of CAT rich in the thylakoid membrane, H2O2 overexpressed by tumor tissue is decomposed into oxygen, the tumor hypoxia microenvironment can be relieved, and the tumor treatment effect can be improved.

The invention belongs to the technical field of biological medicine, and discloses preparation of a Zr-based heterojunction sound-sensitive agent and application of the Zr-based heterojunction sound-sensitive agent in synergistic tumor therapy, and the preparation process comprises the following steps: dissolving H2BDC and ZrOCl2. 8H2O in DMF, adding glacial acetic acid, heating, centrifuging and washing to obtain Zr-MOF; the preparation method comprises the following steps: dissolving EDTA-FeNa powder in deionized water, and carrying out ultrasonic mixing, annealing, centrifuging and filtering to obtain Fe-CDs; and finally, the Zr-MOF, the DOX and the Fe-CDs are mixed, and the DOX / Zr-MOF / Fe-CDs are prepared. The DOX / Zr-MOF / Fe-CDs nano platform is formed by combining Zr-MOF and Fe-doped carbon dots (Fe-CDs), so that the DOX / Zr-MOF / Fe-CDs nano platform has excellent sonodynamic and chemical dynamic properties. The nano platform generates a large amount of reactive oxygen species (ROS) under the action of ultrasound, glutathione (GSH) is indirectly consumed through a Fenton reaction, and meanwhile Zr-MOF serves as a protective layer of degradation behaviors, so that DOX / Zr-MOF / Fe-CDs have controllable degradation behaviors. The invention solves the problems that the existing sonosensitizer ROS is low in yield and easy to consume by GSH, and is suitable for tumor treatment.

The invention relates to a method for promoting nerve cell proliferation and differentiation based on ultrasonic stimulation and application, and belongs to the technical field of biomedicine. According to the method, under the conditions that the power is 90-110 mW and the frequency is 0.9-1.1 MHz, nerve cells are continuously stimulated for 3 days by ultrasonic waves once per day, and each time lasts for 2-4 min. The purposes of increasing the nerve cell proliferation speed and promoting nerve cell differentiation are achieved only through ultrasonic stimulation under specific conditions for the first time, no other substances are added, non-invasion and non-contact are achieved, and toxic and side effects on cells are low; the nerve cells are not subjected to invasive or modified operation; the method has a wide application prospect only through physical conditions.

The invention provides a preparation method of an acoustic / photosensitizer-manganese dioxide nanoparticle and a product thereof. According to the method, firstly, a potassium permanganate reduction method is adopted to precisely synthesize the high-purity MnO2 nano-substrate, and according to the method, the size uniformity and dispersion stability of nano-particles can be precisely controlled by regulating and controlling the pH value and temperature of a reaction system and the concentration of a reducing agent. On the basis, amphiphilic high-molecular polyethylene glycol-polycaprolactone (PEG-PCL) is modified on the surface of the MnO2 nanometre. The whole preparation process is green and mild, and complex equipment is not needed; the reaction conditions are mild, nanoparticles are uniform, and the acoustic / photosensitizer can be combined and applied to an imaging technology to realize real-time monitoring.

The invention discloses a preparation method and application of a magnetic resonance imaging nanoprobe capable of penetrating pancreatic cancer tumor interstitial substance, and belongs to the technical field of self-driven magnetic resonance imaging nanoprobes. The magnetic resonance imaging nanoprobe Ag / Ag2S-GP capable of penetrating pancreatic cancer tumor interstitial substance can be subjected to oxidation-reduction reaction with H2O2 in a tumor microenvironment to continuously generate oxygen airflow, and the reverse thrust of the airflow autonomously drives the probe to move towards the deep part of the tumor interstitial substance; in addition, the Plectin-1 targeting peptide with the Ag / Ag2S-GP surface modified can also be specifically combined with Plectin-1 protein highly expressed by pancreatic cancer cells, and accurate spatial positioning and monitoring of pancreatic cancer are achieved under the guidance of MRI (Magnetic Resonance Imaging). Meanwhile, Ag / Ag2S-GP reduces Schottky barriers under the piezoelectric catalysis action of ultrasonic excitation, hydroxyl radicals are generated, tumor cell apoptosis is induced, and therefore the pancreatic cancer diagnosis and treatment effect is enhanced.

The invention generally relates to sonodynamic therapy using microbubble-sonosensitiser complexes and, more specifically, to such therapy for the treatment of deeply-sited tumours and associated metastatic disease. In particular, the invention relates to a combination therapy in which sonodynamic treatment of deeply-sited tumours with microbubble-sonosensitiser complexes is combined with treatment using immune checkpoint inhibitors. It further relates to methods of sonodynamic therapy in which a sonodynamic-induced abscopal response modulates a systemic regression of metastatic disease. In such methods the abscopal response may be further enhanced by co-administration of an immune checkpoint inhibitor. The invention is particularly suitable for the treatment of pancreatic cancer (e.g. pancreatic ductal adenocarcinoma) and associated metastasis.

The invention belongs to the field of biological medicine and ultrasonic medicine, and provides a barium titanate (BTO) piezoelectric material-based ultrasonic responsive targeting nano system (BTO at BAL), which is used for matrix microenvironment remodeling and immunopotentiation of compact tumors such as pancreatic cancer and the like. According to the nanoparticles, oleic acid modified BTO is taken as a core, ROS responsive prodrug molecules containing a small molecule PD-L1 inhibitor BMS1166 and a nitric oxide donor (Arg) 9 are loaded on the surface, and specific targeting on pancreatic cancer cells is realized by combining a targeting peptide LFC131. Under ultrasonic excitation, BTO generates reactive oxygen species (ROS), on one hand, (Arg) 9 is oxidized to release NO, tumor matrix is degraded, tumor hardness is reduced, drug delivery efficiency is improved, and meanwhile, ROS and NO synergistically enhance tumor immunogenicity; on the other hand, the ROS breaks a thioketal bond to release BMS1166, PD-L1 expression is down-regulated, and immunosuppression is reversed. According to the present invention, the significant tumor inhibition and immune activation effects are represented in the animal model, and the clinical transformation potential is provided.

The present invention discloses a type I semi-cyanine photosensitizer, a type I targeted photosensitizer, and a preparation method and application thereof. The semi-cyanine photosensitizer can rapidly generate a large amount of O2 under light and hypoxic conditions. ·‑ , thereby achieving the effect of photodynamic therapy, and having good biocompatibility and biodegradability, it is an effective type I photosensitizer. In addition, the above-mentioned type I photosensitizer is reacted with phosphorus oxychloride in the presence of water to prepare a type I photosensitizer with tumor targeting. It can specifically target alkaline phosphatase, a biomarker highly expressed in tumors. Under the action of alkaline phosphatase, the photosensitizer can be enriched in the tumor area, thereby achieving precise tumor imaging and photodynamic therapy, which can effectively reduce the side effects of the photosensitizer and improve the inhibitory effect of the photosensitizer on tumors.

The invention discloses an aptamer-modified lipid nano delivery platform as well as a preparation method and application thereof, and belongs to the field of biomedicine. The platform is prepared by taking DPPC, DOTAP and cholesterol as basic lipid components, Ce6 as a sound-sensitive agent and Flt3L as an immune agonist, controlling the particle size through gradient extrusion, and coupling cholesterol with an EpCAM aptamer for surface modification. The method has the core advantages that active targeting enrichment of tumors is realized by virtue of the aptamer, tumor cell immunogen cell death (ICD) is induced in combination with a sonodynamic therapy (SDT), and damage-related molecular patterns (DAMPs) are released; meanwhile, Flt3L is released in a tumor microenvironment, collection and activation of type 1 classical dendritic cells (cDC1) are specifically promoted, an'endogenous cDC1 vaccine 'is constructed, and CD8 + T cell mediated anti-tumor immune response is enhanced. Experiments prove that the platform can significantly improve the cDC1 infiltration level of a tumor site, effectively inhibit the progress of prostate cancer (PCa), and provide a new normal form for immune'cold tumor 'treatment.

The invention discloses an acoustic catalyst as well as a preparation method and application thereof, and belongs to the technical field of catalysts and drug therapy. NJU-319 is selected as an acoustic catalyst, NJU-319Pt is synthesized from a porphyrinyl linker (TAPP) and a hexaphenyl triphenyl unit (HFPTP) under a solvothermal condition, and the H2 generation efficiency of NJU-319Pt can be further improved. After intraperitoneal injection, the nano-drug NJU-319Pt catalyzes H2O to be reduced into H2 under ultrasonic irradiation, and meanwhile lactic acid is oxidized into pyruvic acid. The action mechanism comprises: removing ROS and relieving oxidative stress; by inhibiting an NF-kappa B pathway, the transformation of macrophages from M1 phenotype to M2 phenotype is promoted, and inflammation is reduced; mitochondrial membrane potential is reduced, EMs cell apoptosis is induced, and focus growth is inhibited. The research provides a brand new EMs treatment strategy, combines acoustic catalysis H2 generation with lactic acid oxidation, and provides a new direction for EMs treatment research.

The invention relates to a copper-based heterojunction sound-sensitive agent and a preparation method and application thereof, Cu2O is etched by Se2-to obtain hollow Cu2-xSe, and then graphene quantum dots are loaded on the surface of the Cu2-xSe to obtain the copper-based heterojunction sound-sensitive agent. The copper-based heterojunction sonosensitizer prepared by the preparation method disclosed by the invention not only has efficient sonodynamic and chemical dynamic properties, but also has the capability of consuming glutathione (GSH) in a tumor microenvironment. In addition, the heterojunction sound-sensitive agent can also cause immunotherapy, and strong immune response is caused to achieve the capability of killing tumors.

Described are methods for reversible occlusion of a body lumen by way of degradation as a result of exposure to one or more stimuli such as light. The methods include administering one or more substance(s) into a body lumen of a subject and forming a stimuli-responsive polymer mass in the body lumen from the one or more substance(s). The mass is sufficient to occlude the body lumen in a manner that prevents transport of at least one material through the body lumen and is susceptible to on-command reversal in the body lumen upon exposure to one or more stimuli. The methods include administering one or more stimuli to a polymer mass in a body lumen for a time and intensity to cause the reverse the polymer mass. The methods are particular useful for applications in which it is desirable to temporarily occlude a body lumen, such as male and female contraception.

Disclosed herein are nucleic acid compositions and methods of use. The nucleic acid compositions may have a therapeutic nucleic acid sequence operably linked to a nuclear targeting sequence that increases expression of the therapeutic nucleic acid in a cell by at least 1.25 fold; at least 80% sequence identity to SEQ ID NO: 6; or a first regulatory element comprising a promoter sequence operably linked to a hemoglobin subunit gamma intron (hBGi) sequence, and a second regulatory element comprising a woodchuck hepatitis posttranscriptional regulatory element (WPRE) sequence. The nucleic acid compositions with these features may enhance therapeutic nucleic acid transfection and expression. Also disclosed herein are transgenes optimized for gene therapy applications, including novel FVIII transgene sequences, in which the B domain may be non-naturally occurring the A1 and / or A3 domain may include at least one amino acid substitution.

According to the magnetic multifunctional nano-drug, on the basis of traditional ZIF-8, iron ions and defect structures are introduced, a synergistic antibacterial mode of magnetocaloric therapy-sonodynamic therapy-chemodynamic therapy is established for the first time, heat is generated under an alternating magnetic field, and the magnetic multifunctional nano-drug is used for treating bacterial pneumonia. Active oxygen is rapidly generated under ultrasound, existence of iron ions causes Fenton reaction to achieve chemical power therapy and induce ferroptosis to achieve synergistic antibiosis, release of zinc ions promotes polarization of macrophages to reduce inflammation of disease sites, the problems that focus depth is deep, drug resistance is generated, single treatment effect is poor and the like can be solved, and the treatment effect is good. A new thought is provided for treating the bacterial pneumonia.

The invention discloses a preparation method of a pegylation acoustic catalysis effect black phosphorus loaded biological enzyme nano particle, which is a novel nano composite material based on material chemistry and pharmaceutical chemistry, and bacterial keratitis is treated by using the unique acoustic catalysis property of the nano particle. Nanoparticle synthesis mainly comprises the following steps: 1) pretreatment of commercial black phosphorus; (2) uploading biological enzyme iron-curcumin to black phosphorus in situ; and (3) synthesizing hyaluronic acid coupled phospholipid polyethylene glycol (DSPE-coated PEG2000-HA) and modifying the biological enzyme black phosphorus nanoparticles. The finally obtained nano composite material has good biocompatibility, ocular surface adhesion, biological enzyme activity and acoustic catalytic treatment effect on bacterial biofilm infection of eyes.

The invention discloses a composite nano sound-sensitive agent as well as a preparation method and application thereof. According to the composite nano sound-sensitive agent provided by the invention, a conjugated system of the narrow-gap semiconductor nano sound-sensitive agent core / the ferric oxide nano functional layer / the surface targeting antibody is constructed, so that multi-mode synergy integrating sonodynamic therapy, ferroptosis induction and immunoregulation is realized. The composite nano sonosensitizer further has precise targeting, efficient sonodynamic effect, ferroptosis enhancement effect and good biocompatibility, a targeting antibody on the surface of the composite nano sonosensitizer can specifically recognize high-expression antigens on the surfaces of tumor cells, and dual-targeting enrichment of a treatment system is achieved in combination with magnetic responsiveness guidance; the coating of the iron oxide nanoparticles obviously enhances the sound-sensitive performance of the inner core of the sound-sensitive agent, improves the ROS generation efficiency, and overcomes the hypoxia limitation of the tumor microenvironment; iron oxide is used as an iron source to continuously release Fe < 2 + >, and cooperates with ROS generated by sonodynamic therapy to induce ferroptosis, so that a ferroptosis enhancing effect is generated.

The application belongs to the technical field of medicine, and particularly relates to a La2(WO4)3 / CuWO4 composite sonosensitizer, a preparation method thereof and application thereof in sonodynamic therapy. The La2(WO4)3 / CuWO4 composite sonosensitizer contains 5-40% of La2(WO4)3 in terms of mass percentage. The application takes U251 glioma cells as a model target, and explores the application of the La2(WO4)3 / CuWO4 composite sonosensitizer in sonodynamic therapy of tumors. The results show that the La2(WO4)3 / CuWO4 composite sonosensitizer has a good sonodynamic therapy effect on U251 glioma cell lines after 1MHz US irradiation for 1min.

The present invention discloses an anthraquinone and anthracene compound meso-substituted cyanine dye, its preparation method, and application. The anthraquinone and anthracene compound meso-substituted cyanine dye is prepared by meso-substituting a heptamethine cyanine dye with an anthraquinone or anthracene compound. The substituent groups regulate the fluorescence emission and heat release pathways of the dye, enhancing the photothermal effect of the dye molecule and enabling photoacoustic imaging. Furthermore, its excellent biocompatibility and low cytotoxicity make it suitable for use in the biological and medical fields.

The invention discloses an integrated nano-enzyme with multiple response mechanism synergism, and a preparation method and application thereof. A Z-type heterojunction based on boron nanosheets (B NSs) and rare earth doped iron phosphide (Nd < 0.02 > Fe < 1.98 > P) is constructed for the first time, and CTAB is used for surface modification to obtain a ternary composite nano-enzyme (BFC); according to the present invention, the material is endowed with the multi-response ability (POD / GSHOx enzyme activity synergistic activation) on the bacterial infection microenvironment (low pH and high H2O2), and the nano-enzyme has excellent acoustic-magnetic responsiveness, can break through the depth limitation, and can achieve the precise and controllable generation of the reactive oxygen species (ROS). Under the acidic microenvironment of pulmonary infection, the activity of peroxidase (POD) and glutathione oxidase (GSHOx) is activated, so that efficient and controllable synergistic killing is performed on bacteria.