506results about "Disrupted materials" patented technology

The present invention relates to an immunomicrobubble complex and its uses. The immunomicrobubble complex (IMC) according to the present invention comprises microbubbles conjugated with an antibody, wherein the microbubbles have excellent stability and excellent antibody binding strength, and it has been confirmed that when the immunomicrobubble complex is treated with high-intensity focused ultrasound (HIFU), the anti-tumor effect is significantly enhanced and an immune-enhancing effect is exhibited. Therefore, the immunomicrobubble complex according to the present invention is expected to improve the delivery efficiency of the conjugated antibody and can be used for the diagnosis and treatment of cancer, and exhibits various functions in the field of immunotherapy, including a contrast effect, improvement of half-life, improvement of drug delivery, lymphocyte concentrating effect, cancer immunotherapy, and ultrasound-induced immunotherapy.

The invention belongs to the technical field of biomedical materials, and relates to ultrasonic response adhesion integrated hydrogel as well as a preparation method and application thereof, the hydrogel comprises a hydrogel matrix, an interface anchoring agent and a functional load component; the preparation method comprises the following steps: 1, preparing a hydrogel precursor solution, and preparing a hydrogel matrix into a solution; 2, carrying out mold molding or injection crosslinking on the solution prepared in the step 1 to form hydrogel; 3, preparing an interface anchoring agent solution, and dissolving or dispersing an interface anchoring agent in a solvent to prepare the interface anchoring agent solution; the ultrasonic responsive adhesive hydrogel platform provided by the invention can provide an efficient, controllable and universal solution for tissue adhesion, targeted therapy, wearable attachment and the like in a complex microenvironment, and is suitable for a plurality of clinical scenes such as chronic wound management, surgical postoperative closure, infection control, adhesive bioelectronic fixation, soft tissue engineering and the like.

The present invention relates to sonosensitive liposomes and a method for preparing the same. The sonosensitive liposomes according to the present invention improve the delivery efficiency of encapsulated drugs, and are expected to exhibit various functions in the fields of cancer treatment and drug delivery carriers by improving drug delivery effects through targeted cancer cells when used in combination with ultrasonic treatment.

Hydrogen-bonded framework nanoparticles comprising a chemiluminescent compound (e.g., L-012) are provided and can be used, e.g., for sono-optogenetic treatment of neurodegenerative diseases such as Parkinson's disease. Related in vivo and therapeutic methods are also provided.

The present invention provides ultrasound-responsive liposome nanoparticles and a preparation method and use thereof. The liposome nanoparticles include dimyristoylphosphatidylcholine, 1,2-dioleoyl-3-trimethyl ammonium-propane, distearoylphosphatidylethanolamine-polyethylene glycol, a sonosensitizer, an antibiotic, and perfluoropentane. The liposome nanoparticles are used for the treatment of bacterial biofilm infections. The preparation method involves preparing a liposome film.

The present invention relates to ultrasound (US) mediated delivery of therapeutic agents, such as the delivery of a drug, gene, nanoparticle or radioisotope, using a bi-phasic microparticle system comprising gas microbubbles, emulsion microdroplets and clusters thereof. Thus, the present invention relates to a cluster composition and a pharmaceutical composition, and their use for delivery of therapeutic agents and as a contrast agent for ultrasound imaging. It further relates to methods for delivering such therapeutic agents and to the use of said compositions.

The invention provides dual-response antibacterial repair-promoting injectable hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. Two-dimensional titanium carbide (TiO2 (at) MXene) with the surface modified with titanium dioxide and a bioactive substance are introduced into a hydrogel matrix to serve as functional additives, TiO2 (at) MXene is a photo-thermal and ultrasonic response material, can emit heat under 808 nm near-infrared (NIR) light irradiation and low-intensity pulsed ultrasound (LIPUS) stimulation, and can generate reactive oxygen species (ROS) for sterilization under LIPUS stimulation; when the temperature of the hydrogel is increased, the bioactive substances can be slowly released from the hydrogel, and cell proliferation and tissue repair are promoted. The hydrogel provided by the invention can be coated on the surface of skin or injected under soft tissue, so that the functions of resisting bacteria in the early stage of inflammation and promoting tissue repair in the later stage are realized.

The invention provides an ultrasonic activated blood coagulation targeting tumor selective prodrug as well as a preparation method and application thereof. The blood coagulation targeting tumor selective prodrug has a structure as shown in a formula I. The blood coagulation targeting tumor selective prodrug can trigger a blood coagulation cascade reaction by utilizing tumor vascular injury generated by ultrasonic induction so as to realize targeted anchoring of the drug and inhibit off-target diffusion of an active drug; meanwhile, the prodrug is activated by ultrasound, so that the limitation of tumor heterogeneity on the activation of the prodrug can be overcome. According to the technical scheme, selective enrichment of active drugs in tumors can be effectively improved, and toxicity caused by drug off-target is reduced while tumor growth is remarkably inhibited.

The present invention relates to: a temperature-sensitive hydrogel for cancer treatment; a photothermal composition comprising the hydrogel as an active ingredient; and a preparation method for the temperature-sensitive hydrogel for cancer treatment. The temperature-sensitive hydrogel of the present invention includes gold nanostars as active ingredients, and thus can generate heat by light irradiation, thereby exhibiting a photothermal therapy effect. In addition, the hydrogel temperature increases so that the release of nitric oxide (NO) from S-nitrosocysteine can be induced. In addition, the temperature-sensitive hydrogel of the present invention includes S-nitrosocysteine as an active ingredient so that, upon a temperature rise due to a photothermal reaction, the penetration of drugs into a tumor site can be improved by the release of NO, and at the same time, apoptosis of cancer cells can be directly caused; and includes an immunotherapy agent as an active ingredient so that tumor size can be effectively suppressed even with the passage of time. Therefore, as a material that enables complex treatment combined with photothermal therapy and immunotherapy, the temperature-sensitive hydrogel of the present invention having the aforementioned effect can be usefully utilized in the field of medicine for cancer treatment.

The present disclosure related to a method of using high frequency sound waves directed toward an acoustically active particle. The high frequency sound waves may be configured to enlarge the acoustically active particle and oscillate the acoustically active particle, thereby facilitating extravasation of an agent to a tissue compartment of a region of interest.

The invention relates to a multifunctional silica plastid integrating chemotherapy, immunotherapy and gas therapy, and relates to a preparation method of the multifunctional silica plastid and application of the multifunctional silica plastid in tumor diagnosis and treatment. The schematic diagram is as shown in the figure, the membrane components comprise conventional phospholipid and complex lipid, a chemotherapeutic drug doxorubicin (DOX) entrapped on a hydrophilic core and Fe3 (CO) 12 entrapped on a hydrophobic core, and the chemotherapeutic drug can be adjusted according to needs and is suitable for broad-spectrum tumor treatment. The drug-loaded silica plastid can generate part of carbon monoxide in a tumor high-concentration hydrogen peroxide (H2O2) environment and release internally-loaded chemotherapeutic drugs. Under the action of ultrasound (US), the multifunctional silicon plastid can realize fixed-point release of chemotherapeutic drugs DOX and carbon monoxide at a tumor site, the enrichment and uptake of the drugs at the tumor site are greatly improved, in addition, the system is combined with the alphaPD-L1 antibody, the immunosuppressive PD-L1 / PD-1 axis can be blocked, T cells are recruited to attack tumors, meanwhile, the immunosuppressive regulatory T cells (Tregs) are reduced, and the tumor treatment effect is improved. The tumor inhibition can be enhanced; the pulmonary metastasis can be obviously reduced.

The invention belongs to the technical field of biological medicines, and particularly discloses a nano-engineering T cell membrane coated nano-particle as well as a preparation method and application of the nano-engineering T cell membrane coated nano-particle. Nanoparticles of a specific structure are constructed based on a phospholipid bilayer bionic nanometer platform through a membrane fusion technology, immune clearance caused by phagocytosis of the nanoparticles by macrophages can be avoided through disguise of a T cell membrane, and the blood circulation time is prolonged; the functions of T cells are recovered through specific blocking of immune checkpoint molecules loaded on the immune checkpoint molecules on corresponding immune checkpoint ligands on tumor cells; under ultrasonic radiation, the nano-particles are gradually decomposed and release the sound-sensitive agent to generate a large amount of active oxygen, so that immunogenic death of tumor cells is promoted, more cytotoxic T lymphocytes are recruited to infiltrate into tumor parts, and the recovery of T cell mediated immune response function is facilitated. The synergistic effect of the sound-sensitive agent and the immune checkpoint protein can effectively inhibit tumor growth, induce a long-term immune memory effect and prevent tumor metastasis and recurrence.

Theranostic targeted ultrasonic blasting nano-bubbles, a preparation method therefor, and use thereof, wherein the nano-bubbles are the targeted drug-gene nano-bubbles miRNA / FTY720 / PFP@PEI-T7 NBs. The nano-bubbles can be used for preparing a thyroid ultrasound diagnostic agent or a drug for treating thyroid cancer.

Disclosed herein is a composition for enhanced ultrasound ablation and methods relating to same. The composition may include a microbubble-microdroplet cluster composition, wherein the microbubble-microdroplet cluster composition may include groups of negatively charged microbubbles and positively charged microdroplets permanently held together by their opposing electrostatic attractive forces to form a single, agglomerated entity.

Compositions and methods for administration of local anesthetics that are delivered by a single injection and enable repeated on-demand or high influx analgesia over extended periods have been developed. Pharmaceutical compositions including an effective amount of one or more sodium channel blockers including site 1 sodium channel blockers, optionally one or more alpha-2-adrenergic agonists, which are optionally encapsulated in liposomes, particles or microbubbles, and one or more triggerable elements are provided. The triggerable elements allow delivery of the encapsulated anesthetic drugs when an appropriate triggering stimuli are applied. Exemplary triggering agents or stimuli include near-infrared irradiation, UV- and visible light, ultrasound and magnetic field. In one embodiment, ultrasound is used to trigger a burst of microbubbles to enhance penetration of local anesthetic.

The present invention relates to methods of producing microbubble drug conjugates, viral gene therapy microbubble drug conjugates, and disease-targeting microbubbles, for clinical and preclinical ultrasound-mediated therapeutic and diagnostic applications. It includes methods to produce viral vector gene therapy microbubble drug conjugates with antibody linkers conjugated to lipid shelled microbubbles that both bind to and neutralize viral vectors such that the viral gene therapy can transduce and effect permanent genetic changes only after ultrasound is used to disassociate the viral gene therapy from microbubbles at diseased regions of the body.

The invention belongs to the technical field of medical nano-materials, and particularly relates to a lactoferrin nano-material for sonodynamic regulation and control of blood-brain barrier permeability as well as a preparation method and application of the lactoferrin nano-material. The nanomaterial is nanoparticles LCe6 formed by self-assembly of lactoferrin and chlorin e6, can target brain microvascular endothelial cells, and can temporarily, reversibly and targeting open BBB by using the sonodynamic effect of the LCe6 in combination with transcranial ultrasonic stimulation, so that the accumulation of the nanoparticles LCe6 at a focus part is improved; in addition, the nanoparticle LCe6 also has a targeting property on brain tumor cells, the tumor cells can be accurately killed by utilizing the sonodynamic effect of the nanoparticle under secondary transcranial ultrasound, and the effect of sonodynamic treatment on brain glioma is exerted to the maximum extent; the nanoparticles LCe6 have excellent biocompatibility and low toxicity; according to the invention, the intracerebral delivery efficiency of the drug can be obviously improved, and a novel method for targeting open brain disease BBB is provided.

The invention provides an ultrasonic activated azido precursor, a prodrug and application. The precursor and an active drug react and are bonded to obtain the azido prodrug; the bonding group of the active medicine is amino, hydroxyl or sulfydryl; a bonding group of the ultrasonic activated azido precursor is an ester group. Novel ultrasonic activatable groups, namely p-azidobenzyloxycarbonyl and derivatives thereof, are adopted, hydrogen atoms on a benzene ring are substituted to different degrees, the reducing capacity of a precursor can be remarkably improved, azide is effectively reduced into amino through ultrasonic stimulation under the action of TBR and NADH, and then a self-elimination reaction is induced to release active drugs. The structure has the advantages that different types of active drugs (active pharmacodynamic groups can be amino, hydroxyl and sulfydryl) can be bonded through 4-nitrophenyl carbonate, high-selectivity reduction is performed under the triple action of ultrasound, TBR and NADH, active raw drugs are released according to needs, and toxicity reduction and synergism are achieved.

The invention belongs to the technical field of biological medicines, and particularly discloses a bionic nano-vesicle for regulating and controlling neutrophil phenotype in combination with ultrasound, and a preparation method and application of the bionic nano-vesicle. The bionic nano-vesicle provided by the invention can be broken under the action of high-concentration glutathione existing in a tumor tissue microenvironment to release the sound-sensitive agent and functional siRNA, and under the action of US irradiation, the sound-sensitive agent is activated to generate a large amount of ROS, promote tumor cell DNA breakage, generate a large amount of double-stranded DNA, activate APC and promote IFN-beta release, so that the tumor tissue microenvironment is inhibited. The released functional siRNA can promote the N2-type neutrophile granulocyte to become N1-type neutrophile granulocyte, and the released functional siRNA can prevent the N1-type neutrophile granulocyte from being reversed to the N2-type neutrophile granulocyte again, so that the regulation and control on the phenotype of the neutrophile granulocyte are realized; under catalysis of CAT rich in the thylakoid membrane, H2O2 overexpressed by tumor tissue is decomposed into oxygen, the tumor hypoxia microenvironment can be relieved, and the tumor treatment effect can be improved.

The invention provides a preparation method of a drug carrier combined with ultrasonic transdermal drug delivery, according to the method, a micro-fluidic chip is used, an oil phase solution and a water phase solution can be accurately treated under a micro-scale through a micro-fluidic technology, drug carriers with different particle sizes are prepared, the particle sizes are uniform and controllable, and the repeatability is good. The drug carrier disclosed by the invention can be combined with ultrasound to realize accurate transdermal drug delivery, and under the action of the ultrasound, according to the obvious difference of the drug carriers with different characteristics in rupture time and acting force, the delivery sequence, depth and structural layer of the content of the drug carrier are accurately adjusted, so that the controllable release and accurate delivery of the drug are realized.

The invention belongs to the technical field of biological medicine, and discloses preparation of a Zr-based heterojunction sound-sensitive agent and application of the Zr-based heterojunction sound-sensitive agent in synergistic tumor therapy, and the preparation process comprises the following steps: dissolving H2BDC and ZrOCl2. 8H2O in DMF, adding glacial acetic acid, heating, centrifuging and washing to obtain Zr-MOF; the preparation method comprises the following steps: dissolving EDTA-FeNa powder in deionized water, and carrying out ultrasonic mixing, annealing, centrifuging and filtering to obtain Fe-CDs; and finally, the Zr-MOF, the DOX and the Fe-CDs are mixed, and the DOX / Zr-MOF / Fe-CDs are prepared. The DOX / Zr-MOF / Fe-CDs nano platform is formed by combining Zr-MOF and Fe-doped carbon dots (Fe-CDs), so that the DOX / Zr-MOF / Fe-CDs nano platform has excellent sonodynamic and chemical dynamic properties. The nano platform generates a large amount of reactive oxygen species (ROS) under the action of ultrasound, glutathione (GSH) is indirectly consumed through a Fenton reaction, and meanwhile Zr-MOF serves as a protective layer of degradation behaviors, so that DOX / Zr-MOF / Fe-CDs have controllable degradation behaviors. The invention solves the problems that the existing sonosensitizer ROS is low in yield and easy to consume by GSH, and is suitable for tumor treatment.

The invention relates to a method for promoting nerve cell proliferation and differentiation based on ultrasonic stimulation and application, and belongs to the technical field of biomedicine. According to the method, under the conditions that the power is 90-110 mW and the frequency is 0.9-1.1 MHz, nerve cells are continuously stimulated for 3 days by ultrasonic waves once per day, and each time lasts for 2-4 min. The purposes of increasing the nerve cell proliferation speed and promoting nerve cell differentiation are achieved only through ultrasonic stimulation under specific conditions for the first time, no other substances are added, non-invasion and non-contact are achieved, and toxic and side effects on cells are low; the nerve cells are not subjected to invasive or modified operation; the method has a wide application prospect only through physical conditions.

The invention provides a preparation method of an acoustic / photosensitizer-manganese dioxide nanoparticle and a product thereof. According to the method, firstly, a potassium permanganate reduction method is adopted to precisely synthesize the high-purity MnO2 nano-substrate, and according to the method, the size uniformity and dispersion stability of nano-particles can be precisely controlled by regulating and controlling the pH value and temperature of a reaction system and the concentration of a reducing agent. On the basis, amphiphilic high-molecular polyethylene glycol-polycaprolactone (PEG-PCL) is modified on the surface of the MnO2 nanometre. The whole preparation process is green and mild, and complex equipment is not needed; the reaction conditions are mild, nanoparticles are uniform, and the acoustic / photosensitizer can be combined and applied to an imaging technology to realize real-time monitoring.

The invention discloses a preparation method and application of a magnetic resonance imaging nanoprobe capable of penetrating pancreatic cancer tumor interstitial substance, and belongs to the technical field of self-driven magnetic resonance imaging nanoprobes. The magnetic resonance imaging nanoprobe Ag / Ag2S-GP capable of penetrating pancreatic cancer tumor interstitial substance can be subjected to oxidation-reduction reaction with H2O2 in a tumor microenvironment to continuously generate oxygen airflow, and the reverse thrust of the airflow autonomously drives the probe to move towards the deep part of the tumor interstitial substance; in addition, the Plectin-1 targeting peptide with the Ag / Ag2S-GP surface modified can also be specifically combined with Plectin-1 protein highly expressed by pancreatic cancer cells, and accurate spatial positioning and monitoring of pancreatic cancer are achieved under the guidance of MRI (Magnetic Resonance Imaging). Meanwhile, Ag / Ag2S-GP reduces Schottky barriers under the piezoelectric catalysis action of ultrasonic excitation, hydroxyl radicals are generated, tumor cell apoptosis is induced, and therefore the pancreatic cancer diagnosis and treatment effect is enhanced.

The invention generally relates to sonodynamic therapy using microbubble-sonosensitiser complexes and, more specifically, to such therapy for the treatment of deeply-sited tumours and associated metastatic disease. In particular, the invention relates to a combination therapy in which sonodynamic treatment of deeply-sited tumours with microbubble-sonosensitiser complexes is combined with treatment using immune checkpoint inhibitors. It further relates to methods of sonodynamic therapy in which a sonodynamic-induced abscopal response modulates a systemic regression of metastatic disease. In such methods the abscopal response may be further enhanced by co-administration of an immune checkpoint inhibitor. The invention is particularly suitable for the treatment of pancreatic cancer (e.g. pancreatic ductal adenocarcinoma) and associated metastasis.

The invention provides sonodynamic targeted drug-loaded nanoparticles as well as a preparation method and application thereof, and belongs to the field of targeted drug delivery preparations. The sonodynamic targeting drug-loading nanoparticle provided by the invention comprises a porous carrier, a drug loaded in the porous carrier and a targeting ligand chemically modified on the outer surface of the porous carrier, the porous carrier is a hydrogen bond organic framework; a construction unit of the hydrogen bond organic framework is a metalloporphyrin complex. After the sonodynamic targeting drug-loading nano-particle provided by the invention is injected into a 4T1 tumor-bearing BALB / c mouse body for 6 hours, fluorescence of the sonodynamic targeting drug-loading nano-particle is observed on a tumor, and a signal is continuously enhanced; after 14 days of administration, the tumor growth is obviously inhibited, and good targeting and anti-tumor effects are achieved.

The invention relates to a preparation method and application of a targeted pancreatic cancer immune checkpoint diagnosis and treatment integrated nanoprobe, which can realize integration of sonodynamic therapy and MRI imaging of pancreatic cancer cells with high expression of VISTA, realize targeted SDT therapy of pancreatic cancer and visual evaluation of the therapeutic effect, have good biological safety, and can be applied to diagnosis and treatment of pancreatic cancer cells with high expression of VISTA. SDT visual treatment of targeted pancreatic cancer is realized at a cellular level, and SDT combined immunotherapy of pancreatic cancer is realized at an animal level. The method comprises the following steps: (1) preparing Fe3O4 nanoparticles by using a solvothermal method; (2) preparing Fe3O4 (at) SiO2 nano particles; (3) preparing Fe3O4 (at) SiO2 (at) TiO2 nano particles; (4) preparing Fe3O4 (at) TiO2 by adopting an alkaline hydrothermal corrosion auxiliary crystallization method; and (5) modifying the nanoprobe with a VISTA antibody through an electrostatic adsorption method, and finally successfully constructing the garland-shaped nanoprobe.

Compositions and methods are provided for ultrasound-triggered drug release that enables high drug loading potential for varied drugs of interest, has minimal drug release without ultrasound, can be activated following intravenous administration, and utilizes an ultrasound-activation mechanism that is mechanically-based, without requiring heating above body temperature or cavitation, and is activated with a low duty cycle and shorter ultrasound protocol than is used for heat or sonodynamic activation. These parameters are accomplished by tuning the acoustic impedance and osmolarity of the liquid core of an acoustically activatable particle, e.g. liposomes or emulsions, to maximize ultrasound responsiveness while maintaining overall stability of the structure in the absence of ultrasound.

The invention provides an antibacterial, anti-inflammatory and osteogenesis-promoting two-dimensional bimetallic MOF (Metal Organic Framework) as well as preparation and application thereof. The two-dimensional bimetal MOF can effectively resist bacteria and inflammation, has good biological safety and long-acting function exerting capacity, can efficiently remove bacteria under the action of low-strength medical US before degradation, and can exert the function of removing ROS through nano-enzyme activity. After degradation, copper and manganese ions slowly released by the two-dimensional bimetal MOF and quercetin can be chelated, SOD and CAT-like effects are achieved through metal-ion charge transfer MLCT, the ROS adsorption effect can be continuously achieved, and in addition, due to the fact that the copper and manganese ions and quercetin have osteogenesis and antibacterial effects, bone tissue repair can be continuously and efficiently promoted.

The invention belongs to the field of biological medicine and ultrasonic medicine, and provides a barium titanate (BTO) piezoelectric material-based ultrasonic responsive targeting nano system (BTO at BAL), which is used for matrix microenvironment remodeling and immunopotentiation of compact tumors such as pancreatic cancer and the like. According to the nanoparticles, oleic acid modified BTO is taken as a core, ROS responsive prodrug molecules containing a small molecule PD-L1 inhibitor BMS1166 and a nitric oxide donor (Arg) 9 are loaded on the surface, and specific targeting on pancreatic cancer cells is realized by combining a targeting peptide LFC131. Under ultrasonic excitation, BTO generates reactive oxygen species (ROS), on one hand, (Arg) 9 is oxidized to release NO, tumor matrix is degraded, tumor hardness is reduced, drug delivery efficiency is improved, and meanwhile, ROS and NO synergistically enhance tumor immunogenicity; on the other hand, the ROS breaks a thioketal bond to release BMS1166, PD-L1 expression is down-regulated, and immunosuppression is reversed. According to the present invention, the significant tumor inhibition and immune activation effects are represented in the animal model, and the clinical transformation potential is provided.