227results about "Disrupted materials" patented technology

The invention provides an ultrasonic activated blood coagulation targeting tumor selective prodrug as well as a preparation method and application thereof. The blood coagulation targeting tumor selective prodrug has a structure as shown in a formula I. The blood coagulation targeting tumor selective prodrug can trigger a blood coagulation cascade reaction by utilizing tumor vascular injury generated by ultrasonic induction so as to realize targeted anchoring of the drug and inhibit off-target diffusion of an active drug; meanwhile, the prodrug is activated by ultrasound, so that the limitation of tumor heterogeneity on the activation of the prodrug can be overcome. According to the technical scheme, selective enrichment of active drugs in tumors can be effectively improved, and toxicity caused by drug off-target is reduced while tumor growth is remarkably inhibited.

Theranostic targeted ultrasonic blasting nano-bubbles, a preparation method therefor, and use thereof, wherein the nano-bubbles are the targeted drug-gene nano-bubbles miRNA / FTY720 / PFP@PEI-T7 NBs. The nano-bubbles can be used for preparing a thyroid ultrasound diagnostic agent or a drug for treating thyroid cancer.

The invention belongs to the technical field of biological medicines, and particularly discloses a bionic nano-vesicle for regulating and controlling neutrophil phenotype in combination with ultrasound, and a preparation method and application of the bionic nano-vesicle. The bionic nano-vesicle provided by the invention can be broken under the action of high-concentration glutathione existing in a tumor tissue microenvironment to release the sound-sensitive agent and functional siRNA, and under the action of US irradiation, the sound-sensitive agent is activated to generate a large amount of ROS, promote tumor cell DNA breakage, generate a large amount of double-stranded DNA, activate APC and promote IFN-beta release, so that the tumor tissue microenvironment is inhibited. The released functional siRNA can promote the N2-type neutrophile granulocyte to become N1-type neutrophile granulocyte, and the released functional siRNA can prevent the N1-type neutrophile granulocyte from being reversed to the N2-type neutrophile granulocyte again, so that the regulation and control on the phenotype of the neutrophile granulocyte are realized; under catalysis of CAT rich in the thylakoid membrane, H2O2 overexpressed by tumor tissue is decomposed into oxygen, the tumor hypoxia microenvironment can be relieved, and the tumor treatment effect can be improved.

The invention provides a preparation method of an acoustic / photosensitizer-manganese dioxide nanoparticle and a product thereof. According to the method, firstly, a potassium permanganate reduction method is adopted to precisely synthesize the high-purity MnO2 nano-substrate, and according to the method, the size uniformity and dispersion stability of nano-particles can be precisely controlled by regulating and controlling the pH value and temperature of a reaction system and the concentration of a reducing agent. On the basis, amphiphilic high-molecular polyethylene glycol-polycaprolactone (PEG-PCL) is modified on the surface of the MnO2 nanometre. The whole preparation process is green and mild, and complex equipment is not needed; the reaction conditions are mild, nanoparticles are uniform, and the acoustic / photosensitizer can be combined and applied to an imaging technology to realize real-time monitoring.

The invention belongs to the field of biological medicine and ultrasonic medicine, and provides a barium titanate (BTO) piezoelectric material-based ultrasonic responsive targeting nano system (BTO at BAL), which is used for matrix microenvironment remodeling and immunopotentiation of compact tumors such as pancreatic cancer and the like. According to the nanoparticles, oleic acid modified BTO is taken as a core, ROS responsive prodrug molecules containing a small molecule PD-L1 inhibitor BMS1166 and a nitric oxide donor (Arg) 9 are loaded on the surface, and specific targeting on pancreatic cancer cells is realized by combining a targeting peptide LFC131. Under ultrasonic excitation, BTO generates reactive oxygen species (ROS), on one hand, (Arg) 9 is oxidized to release NO, tumor matrix is degraded, tumor hardness is reduced, drug delivery efficiency is improved, and meanwhile, ROS and NO synergistically enhance tumor immunogenicity; on the other hand, the ROS breaks a thioketal bond to release BMS1166, PD-L1 expression is down-regulated, and immunosuppression is reversed. According to the present invention, the significant tumor inhibition and immune activation effects are represented in the animal model, and the clinical transformation potential is provided.

The invention discloses an aptamer-modified lipid nano delivery platform as well as a preparation method and application thereof, and belongs to the field of biomedicine. The platform is prepared by taking DPPC, DOTAP and cholesterol as basic lipid components, Ce6 as a sound-sensitive agent and Flt3L as an immune agonist, controlling the particle size through gradient extrusion, and coupling cholesterol with an EpCAM aptamer for surface modification. The method has the core advantages that active targeting enrichment of tumors is realized by virtue of the aptamer, tumor cell immunogen cell death (ICD) is induced in combination with a sonodynamic therapy (SDT), and damage-related molecular patterns (DAMPs) are released; meanwhile, Flt3L is released in a tumor microenvironment, collection and activation of type 1 classical dendritic cells (cDC1) are specifically promoted, an'endogenous cDC1 vaccine 'is constructed, and CD8 + T cell mediated anti-tumor immune response is enhanced. Experiments prove that the platform can significantly improve the cDC1 infiltration level of a tumor site, effectively inhibit the progress of prostate cancer (PCa), and provide a new normal form for immune'cold tumor 'treatment.

Disclosed herein are nucleic acid compositions and methods of use. The nucleic acid compositions may have a therapeutic nucleic acid sequence operably linked to a nuclear targeting sequence that increases expression of the therapeutic nucleic acid in a cell by at least 1.25 fold; at least 80% sequence identity to SEQ ID NO: 6; or a first regulatory element comprising a promoter sequence operably linked to a hemoglobin subunit gamma intron (hBGi) sequence, and a second regulatory element comprising a woodchuck hepatitis posttranscriptional regulatory element (WPRE) sequence. The nucleic acid compositions with these features may enhance therapeutic nucleic acid transfection and expression. Also disclosed herein are transgenes optimized for gene therapy applications, including novel FVIII transgene sequences, in which the B domain may be non-naturally occurring the A1 and / or A3 domain may include at least one amino acid substitution.

The application belongs to the technical field of medicine, and particularly relates to a La2(WO4)3 / CuWO4 composite sonosensitizer, a preparation method thereof and application thereof in sonodynamic therapy. The La2(WO4)3 / CuWO4 composite sonosensitizer contains 5-40% of La2(WO4)3 in terms of mass percentage. The application takes U251 glioma cells as a model target, and explores the application of the La2(WO4)3 / CuWO4 composite sonosensitizer in sonodynamic therapy of tumors. The results show that the La2(WO4)3 / CuWO4 composite sonosensitizer has a good sonodynamic therapy effect on U251 glioma cell lines after 1MHz US irradiation for 1min.

The invention belongs to the field of pathologies affecting the central nervous system: in particular, severe cerebral pathologies, more particularly those restricted by the presence of the blood-brain barrier (BBB): gliomas, cerebral metastases, neurodegenerative diseases (e.g. Alzheimer's, Parkinson's or ALS), genetic diseases (Huntington's, myopathies, Leigh syndrome, Rett syndrome), but also to the field of cancers, musculoskeletal and immunological disorders, vascular diseases (thrombus) in numerous organs (e.g. liver, kidney or muscle) and in combination with numerous therapeutic approaches (e.g. chemotherapy, immunotherapy, targeted therapy or gene therapy). The invention relates to a microbubble comprising a fluorinated polymer or copolymer and a fluorinated gas, to the use thereof and also to the polymer or copolymer intermediate compounds.

The application discloses a kind of nanoparticles for tumor sonodynamic combined individualized immunotherapy and its preparation method and application, belong to the field of biological medicine.The nanoparticles for tumor sonodynamic combined individualized immunotherapy are double-shell core structure nanoparticles formed by inner core and shell, and the inner core is formed by self-assembly of TLR3 agonist Poly (I:C), L-arginine and polylysine, and the shell is hyaluronic acid layer HA-Ce6 conjugated with photosensitizer Ce6.It is found through experiment verification that the nanoparticles can target tumor, generate reactive oxygen species to kill tumor cells under the action of ultrasound, promote M1 macrophage polarization and produce NO release at the same time, play the role of antitumor and immune effect, and realize the combined application of sonodynamic and NO gas therapy.The nanoparticles for tumor sonodynamic combined individualized immunotherapy can trigger systemic immune response, inhibit primary tumor, produce immune memory effect, and prevent tumor metastasis and recurrence.

The invention relates to a bimetallic alloy nano-enzyme for treating multi-drug-resistant bacterial infection as well as a preparation method and application thereof, and belongs to the field of biomedical nano-materials. The problems that in the prior art, the catalytic activity of metal and metal-based nano enzyme is limited, the bactericidal effect is poor due to poor targeting performance, the bactericidal effect in vivo (especially deep drug-resistant bacteria) is poor, and biocompatibility is poor are solved. The invention relates to a bimetallic alloy nano-enzyme for treating multidrug-resistant bacterial infection, which is mainly composed of two metal elements of Pt and Ru, and the atomic ratio of Pt / Ru is 90 / 10-70 / 30. The bimetallic alloy nano-enzyme disclosed by the invention has efficient biological catalytic activity, has a very good treatment effect on deep drug-resistant bacterial infection under ultrasonic activation, shows remarkable antibacterial, anti-biofilm and anti-inflammatory multiple effects, and is good in biocompatibility.

The invention provides a diagnosis and treatment integrated nano platform as well as a preparation method and application thereof. According to the diagnosis and treatment integrated nano platform, an organic-inorganic nano hybrid is constructed through structure optimization and function integration, four modes of PTT, PDT, SDT and CDT are organically combined innovatively, and efficient synergistic treatment of GBM is achieved through real-time monitoring of photoacoustic imaging. First, a dOMV encapsulation strategy significantly improves the ability to penetrate through the blood brain barrier. Meanwhile, the IEICO-4F has a strong near-infrared absorption characteristic and has a synergistic effect with the high catalytic activity of the mesoporous platinum nanoparticles. Under the laser / ultrasonic excitation condition, the diagnosis and treatment integrated nano platform shows excellent photo-thermal conversion performance, the Fenton reaction rate can be increased, endogenous hydrogen peroxide can be continuously catalyzed to be decomposed to generate oxygen, and the tumor hypoxia state is effectively relieved. The series of reactions further enhance the generation of ROS, activate a Caspase-1 / GSDMD mediated pyroptosis pathway, finally achieve an efficient tumor inhibition effect, and successfully break through the limitation of a single treatment mode.

Disclosed are methods of delivering a therapeutic to a target site of a subject comprising administering a lipid capsule to a target site of a subject, wherein the lipid capsule comprises a therapeutic agent; and applying an alternating electric field, at a frequency for a period of time, to the target site of the subject, wherein the alternating electric field releases the therapeutic from lipid capsule at the target site of the subject. Disclosed are methods of increasing target site specific release of a therapeutic agent in a subject comprising administering a lipid capsule to a target site of a subject, wherein the lipid capsule comprises a therapeutic agent; and applying an alternating electric field, at a frequency for a period of time, to the target site of the subject, wherein the alternating electric field releases the therapeutic agent from the lipid capsule at the target site of the subject, thereby increasing the target site specific release of the therapeutic agent. Disclosed are methods of treating comprise administering a lipid capsule to a target site of a subject in need thereof, wherein the lipid capsule comprises a therapeutic agent; and applying an alternating electric field, at a frequency for a period of time, to the target site of the subject in need thereof, wherein the alternating electric field releases the therapeutic agent from the lipid capsule at the target site of the subject in need thereof. Disclosed are methods of killing a cell comprising administering a lipid capsule to a target site, wherein the lipid capsule comprises a therapeutic agent; and applying an alternating electric field for a period of time, to the target site, wherein the alternating electric field releases the therapeutic agent from the lipid capsule at the target site, wherein the target site comprises a cell, wherein the therapeutic kills the cell.

The invention provides a cobalt-iridium metal complex, nanoparticles, a preparation method and application, and belongs to the technical field of photodynamic therapy drugs. The structural formula of the cobalt-iridium metal complex is shown as a formula (I). The invention also provides a preparation method of the cobalt-iridium metal complex. The invention also provides nanoparticles, the cobalt-iridium metal complex prepared by the invention has a chemiluminescence property, so that the cobalt-iridium metal complex can simultaneously realize photo-thermal, photodynamic and ultrasonic synergistic thrombolysis treatment effects, and the oxidation resistance of the cobalt-iridium metal complex has a remarkable curative effect on a vascular injury part. The complex provided by the invention has good photo-thermal, photodynamic and ultrasonic response capabilities, and can overcome the problems of short excitation wavelength of exciting light and limited light penetration depth required by the existing metal iridium complex. Experiments show that the material has a good treatment effect on thrombus.

The invention relates to the field of biological medicine, in particular to a nanoparticle for treating myocardial ischemia reperfusion injury, a preparation method and application. According to the scheme, the nanoparticles comprise liposome containing ML351, a phase change material PFP is sealed in the liposome, and a TA-Ce metal polyphenol network coating is wrapped outside the liposome. The TA-Ce coating ensures that the system actively anchors myocardial cells through multiple acting forces, continuously removes ROS, and creates a primary protection environment for the myocardial cells. Under ultrasonic triggering, the PFP in the inner core is subjected to phase change blasting, local enrichment and accurate release of ML351 are realized, lipid peroxide is reduced, a ferroptosis pathway is blocked, and sequential treatment under space-time control is realized. A network structure of TA-Ce removes ROS, and TA-Ce and ML351 inhibit lipid peroxidation, and upstream and downstream synergy is formed on an anti-oxidation and anti-ferroptosis pathway, so that the overall treatment effect is greatly enhanced.

The application relates to a kind of nanocomposites, comprising: carrier;Silver;Vancomycin (Van);And hemin chloride (Hemin);Wherein, the silver, vancomycin (Van) and hemin chloride (Hemin) are loaded on carrier;Wherein the nanocomposite releases silver ion, vancomycin and hemin chloride under near infrared light irradiation.The nanocomposite of the application uses nanomaterial as carrier, and can achieve the purpose of high-efficiency sterilization by the loading of silver combined with Van in hollow cavity.At the same time, hemin chloride can realize photo-thermal conversion in cooperation with silver, and effectively remove redundant active oxygen.The successful implementation of the nanocomposite will lay a foundation for constructing efficient and safe antibacterial strategy and provide a new idea for pathogen infection treatment.

The invention belongs to the cross technical field of cell engineering, nano-drugs and biological manufacturing, and particularly discloses a drug-loaded vesicle based on denucleated cells as well as a preparation method and application of the drug-loaded vesicle. According to the invention, a cell suspension and a cytoskeleton relaxant are co-incubated to relax an actin skeleton and weaken nucleoplasm connection; then loading the treated cell suspension on a multi-layer discontinuous density gradient centrifugal medium, realizing physical separation of cell nucleuses and cytoplasm through high-speed centrifugation according to buoyancy density difference, and collecting components of a specific interface to obtain high-purity denucleated cells with a complete membrane structure; then co-incubating the denucleated cells and ROS response type lipidosome (co-carrying therapeutic siRNA and a sound-sensitive agent Ce6) prepared in advance, so that the lipidosome is wrapped or anchored by a denucleated cell membrane; finally, the composite system is subjected to extrusion treatment through a microporous membrane, the drug-loaded vesicles uniform in particle size and stable in structure are obtained, and the drug-loaded vesicles are suitable for various application scenes such as anti-tumor treatment and RNA vaccine delivery.

The invention provides an ultrasonic response type microsphere preparation entrapped with a protein degradation agent as well as a preparation method and application of the ultrasonic response type microsphere preparation. The microsphere is of a microsphere structure entrapped with a protein degradation agent and formed by a conjugate of a biodegradable polymer and a sound-sensitive agent and the protein degradation agent. According to the invention, the targeted protein degradation agent is entrapped in a biodegradable novel polymer matrix, so that the slow release effect of the medicine is realized, and the long-acting effect is achieved at a specific part in the body. The microsphere combines the respective advantages of an ultrasonic technology and a targeted protein degradation technology, and the two technologies work together, so that the controllability of drug release is remarkably improved, a synergistic effect is achieved, and the treatment effect is improved.

The present invention discloses a multi-substrate adhesive hydrogel capable of efficiently delivering nucleic acid molecules and its application in tumor treatment. The preparation steps of the hydrogel are as follows: preparing a MXene nanosheet solution by hydrofluoric acid etching; preparing a disulfide solid powder by recrystallization; mixing the disulfide solid powder with the MXene nanosheet solution and stirring at room temperature to obtain solution A; mixing CpG oligonucleotide with solution A and dialyzing to obtain an ONMs nanoparticle solution; grafting sodium alginate and amino-modified ATP aptamer to obtain DNA SA solution; ONMs nanoparticle solution and DNA SA solution was mixed and incubated at room temperature to obtain DNA SA@ONMs solution; DNA The SA@ONMs solution was mixed with acrylamide, and then an ionic crosslinker, a crosslinker, a crosslinking accelerator, and an initiator were added in sequence. The mixture was stirred at room temperature and allowed to stand to solidify into a gel, thereby obtaining a multi-substrate adhesion hydrogel.

The invention belongs to the technical field of medicines, and particularly relates to application of a nano sound-sensitive agent in preparation of anti-cancer drugs. The invention provides application of a nano sound-sensitive agent in preparation of anti-cancer drugs. The nano sound-sensitive agent comprises an inorganic piezoelectric semiconductor and transition metal loaded on the inorganic piezoelectric semiconductor. The nano sound-sensitive agent provided by the invention has efficient sound sensitivity, and can effectively inhibit the growth, migration and invasion of cancer cells.

The application provides an antibacterial anti-inflammatory and osteogenesis-promoting two-dimensional bimetallic MOF as well as preparation and application thereof. The MOF can effectively resist bacteria and inflammation, has good biological safety, and has the ability to act for a long time. Before degradation, the two-dimensional bimetallic MOF provided by the application can effectively remove bacteria under the action of low-intensity medical US, and can remove ROS through nano-enzyme activity. After degradation, the copper and manganese ions and quercetin released by the two-dimensional bimetallic MOF can be chelated, and then the metal-ion charge transfer MLCT effect is exerted to play the SOD and CAT effects, so that the ROS adsorption effect can be continuously exerted. In addition, the copper and manganese ions and quercetin can continuously and efficiently promote bone tissue repair due to the osteogenesis and antibacterial effects.

The invention provides methods for enhancing the efficacy of a cancer treatment or treating cancer in a subject by administering a composition comprising an anti-cancer agent and a microbubble suspension. The microbubble suspension is characterized by a gas concentration range of 1.24 pL / kg to 30.57 pL / kg of the subj ect's body weight or a human equivalent dose (HED) range of 2x 108 to 4x 109 bubbles per kg body weight. Ultrasound with a setting of continuous non-thermal pulses with short cycle and sufficient time interval is applied to induce microbubble destruction in a targeted region using continuous non-thermal pulses with short cycles and sufficient time intervals, wherein the mechanical index (MI) value is between 0.4 and 1.2.

The application discloses a naphthalene phthalocyanine copper liposome and a construction method and application thereof, and belongs to the technical field of liposome preparations. The naphthalene phthalocyanine copper liposome can improve the problems of poor water solubility and insufficient stability of existing sonosensitizers by wrapping naphthalene phthalocyanine copper and catalase in the liposome. Meanwhile, the naphthalene phthalocyanine copper liposome has the function of catalyzing the production of O2 by excessive H2O2 in the microenvironment of brain glioma to supply the continuous production of ROS in the sonodynamic therapy (SDT), enhances the sonodynamic performance, improves the treatment effect of brain glioma, has the advantages of good biological safety, low immunogenicity and long circulation in the body, can effectively penetrate the blood-brain barrier and be delivered to the tumor site, solves the problems that drugs are difficult to penetrate the blood-brain barrier and cannot reach an effective concentration in the traditional treatment method of brain glioma, and provides a new strategy for the treatment of brain glioma.

The application discloses a preparation method and application of a magnetic resonance imaging nano probe capable of penetrating pancreatic cancer tumor interstitium and belongs to the technical field of self-driven magnetic resonance imaging nano probes. The magnetic resonance imaging nano probe Ag / Ag2S-GP capable of penetrating pancreatic cancer tumor interstitium can have an oxidation-reduction reaction with H2O2 in a tumor microenvironment, continuously generate oxygen gas flow, and the back thrust of the gas flow can autonomously drive the probe to move to the deep part of the tumor interstitium. The Plectin-1 targeting peptide on the surface of the Ag / Ag2S-GP can also specifically combine with the Plectin-1 protein highly expressed by pancreatic cancer cells, and realize precise spatial positioning and monitoring of the pancreatic cancer under the guidance of MRI. Meanwhile, the Ag / Ag2S-GP can reduce a Schottky barrier under the piezoelectric catalysis of ultrasonic excitation, generate hydroxyl radicals, induce tumor cell apoptosis, and thus enhance the diagnosis and treatment effect of the pancreatic cancer.

The present invention relates to a micelle complex and a drug carrier. By supporting hydrophobic manganese oxide particles in the micelles, the complex is stably protected in vivo and easily delivered to a tumor site. Further, the micelles have a hydrophilic portion and a hydrophobic portion bound by a reactive oxygen species (ROS) cleavable linker in order to be degraded in response to a reactive oxygen species of a target site, and thus can efficiently release the hydrophobic manganese oxide particles. The released hydrophobic manganese oxide particles generate oxygen and changes the microenvironment of the target site, and thus may enhance the therapeutic effect of a drug carried on the micelles.

The invention relates to an iron ion doped trititanium pentoxide material and application thereof in treatment of ferroptosis sensitive tumors. The preparation method of the iron ion doped trititanium pentoxide material comprises the following steps: taking FeCl3. 6H2O and Ti3O5 as raw materials, and carrying out ball milling in a planetary ball mill to prepare a Fe-Ti3O5 composite material; and dispersing the Fe-Ti3O5 composite material and BSA in deionized water under continuous magnetic stirring, finally carrying out centrifugal separation on a final product, and washing to obtain an iron ion doped trititanium pentoxide material which is marked as Fe-Ti3O5-BSA. According to the invention, through a simple and convenient method of doping Fe ions on the surface of Ti3O5, lipid metabolism is regulated, ferroptosis is sensitized, and the Ti3O5 is used for tumor treatment. The iron ion doped trititanium pentoxide material provided by the invention can enhance the ferroptosis effect and the anti-tumor effect, and provides huge potential for developing a ferroptosis sensitive tumor treatment strategy.

The invention belongs to the field of biological materials, and particularly discloses a multi-component microcapsule with multiple ultrasonic responses as well as a preparation method and application of the multi-component microcapsule with the multiple ultrasonic responses, and a hydrogel microcapsule with structural and functional design is prepared through a microfluidic double-emulsion technology on the basis of light-cured methacrylic anhydride gelatin hydrogel and liquid decafluoropentane. According to the prepared multi-ultrasonic-responsive multi-component microcapsule, ultrasonic-responsive oxygen release and controllable generation of reactive oxygen free radicals are realized on the basis of curcumin loaded in methacrylic anhydride gelatin and oxygen dissolved in decafluoropentane; the released oxygen provides an oxygen raw material for sonodynamic therapy while relieving a hypoxic environment in an articular cavity, so that generation of reactive oxygen free radicals is enhanced, apoptosis of pathological synovial fibroblasts is induced, and synovial proliferation in the pathological articular cavity is inhibited.

The invention discloses a preparation method of a piezoelectric MOF (Metal Organic Framework) sound-sensitive agent with an ultrasonic response ROS / CO synergistic antibacterial function. The preparation method comprises the following steps: synthesizing a catechol modified ruthenium carbonyl carbon monoxide donor through a coupling reaction of 3, 4-dihydroxyl-1-phenylalanine and carbonyl ruthenium chloride; the method comprises the following steps: mixing an L-carnosine aqueous solution and a zinc ion aqueous solution in a solvent, and self-assembling the two solutions by a hydrothermal method to obtain a piezoelectric metal organic framework (PMOF); and modifying the carbon monoxide donor on the surface of the PMOF through a complexation reaction between zinc ions on the surface of the PMOF and phenolic hydroxyl groups of the carbon monoxide donor to obtain the piezoelectric MOF sound-sensitive agent with ultrasonic response ROS / CO synergistic antibiosis. The invention also discloses the piezoelectric MOF sonosensitizer with ultrasonic response ROS / CO synergistic antibacterial effect obtained by the preparation method and application of the piezoelectric MOF sonosensitizer in preparation of sonosensitizer hydrophobic drugs and / or antibacterial agents. The sonosensitizer provided by the invention has excellent biocompatibility, can controllably start release of active oxygen and carbon monoxide through ultrasound to achieve synergistic antibiosis, enhances the antibacterial ability, and has excellent biological membrane penetrating ability.

The application belongs to the technical field of biomedical materials, and particularly relates to a supramolecular coordination complex with sonodynamic activity and a preparation method and application thereof. The application proposes a new supramolecular coordination complex formed by self-assembly of a phthalocyanine compound and Ru-dbq, and finds that the supramolecular coordination complex has excellent ROS yield in response to US, and also has the characteristics of multifunctional integration such as CAT enzyme-like activity and antioxidant activity, which endows the material with great application potential in response to complex biological environments and excellent sonodynamic therapy prospects.

The present application relates to a new use of fluorocarbon phase transition microparticles as a therapeutic ablation agent for mechanically damaging or ablating diseased tissue. When fluorocarbon phase transition microparticles are in contact with diseased tissue, under the excitation of low-frequency focused ultrasound, gas-liquid phase transition (i.e. vaporization) and ultrasonic cavitation resonance of vaporized microbubbles occur, followed by controlled intensity cavitation and mechanical damage of local tissue, thereby producing an ablation therapeutic effect on diseased tissue. Vaporized fluorocarbon phase transition microbubbles become ideal exogenous cavitation nuclei, which can significantly reduce the cavitation threshold and induce enhanced inertial cavitation effects. This method has the characteristics of short treatment time, low required ultrasonic pressure and sound intensity, minimal damage to tissues outside the target area, easy operation, etc., improving the targeting and safety of treatment. Although this treatment method requires image guidance, it does not require very precise focusing, reducing the difficulty of operation, and has application prospects in multiple clinical treatment fields such as prostate hyperplasia and tumor immunotherapy.