758 results about "Immunopotentiation" patented technology

The present invention provides immunosuppression compounds capable of inhibiting the programmed cell death 1 (PD1) signalling pathway. The present invention further provides peptide based compositions for treatment of cancer or treatment of infections via immunopotentiation caused by inhibition of immunosuppressive signaling induced by PD-1, PD-L1, or PD-L2 and therapies using them, immunopotentiative substrates included as the active ingredient. Further, the invention provides an application of the compositions containing the peptide moieties for preventive and/or therapeutic agents for cancer, cancer metastasis, immunodeficiency, an infectious disease or the like and an application of peptide moieties as a testing or diagnostic agent or a research agent for such a disease.

Disclosed are immunopotentiating agents, and vaccines thereof, which enhance and/or otherwise modify immune responses, and method for their preparation and use in vivo. Immunopotentiating agents can be single agents that act directly, adjuvants added concurrently with the agents, or heteroconjugates wherein the immunopotentiating agent is chemically coupled to the compound against which an immune response is desired. Examples of immunopotentiating agents include monoclonal antibodies, such as anti-CD3, anti-CD2) and anti-CD5 antibodies, and proteins derived from microorganisms (e.g., enterotoxins) which activate T cells. The compounds against which an immune response can be generated, which may be the second component in a heteroconjugate, include compound from abnormal or diseased tissues such as tumors, or infectious agents, such as viruses, bacteria, fungi, protozoal or metozoal parasites, and can be obtained by natural or recombinant means. Methods of using the invention to prepare monoclonal antibodies are particularly disclosed.

Methods of stimulating an immune response and treating patients responsive thereto with 3,4-di(1H-indol-3-yl)-1H-pyrrole-2,5-diones, staurosporine analogs, derivatized pyridazines, chromen-4-ones, indolinones, quinazolines, nucleoside analogs, and other small molecules are disclosed. In a preferred embodiment benzopyrimidine derivatives such as ZD-6474, MLN-518, lapatinib, gefitinib or erlotinib are used.

The invention relates, in part, to a method of treating a subject comprising administering to the subject a low, immune enhancing of a mTOR inhibitor and an immune effector cell engineered to express a CAR.

The invention relates to diagnostic methods for assessing the need of a subject for treatment with an anti-oxidant, or alternatively, for determining the utilization efficiency and ultimate effectiveness of anti-oxidant therapy in subjects having been treated with antioxidants. More specifically, the methods of the present invention are particularly useful in prophylactic assessment of individuals at risk for developing diseases or conditions in which oxidative stress plays a role, such that an appropriate therapeutic regimen can be prescribed for that individual, thus leading to alternative therapies and/or life style changes. The invention further relates to methods for assessing the need for, the utilization efficiency and the effectiveness of therapy in subjects having received therapy with specific antioxidant and immune enhancing formulations. Kits are also provided for measuring the levels of markers of oxidative stress and immune cell numbers.

The invention relates to a Chinese herbal medicine biological feed additive for livestock and a preparation method of the Chinese herbal medicine biological feed additive. The prepared Chinese herbal medicine biological feed additive for the livestock comprises the following raw materials in percentage by mass: 20% of probiotics, 10% of an enzyme preparation, 50% of traditional Chinese medicines, 10% of amino acids, 4% of vitamins, 1% of micro elements, 1% of an immunopotentiator, 1% of bacitracin and 3% of glucose. The preparation method comprises the following steps: firstly carrying out expanding culture to revive the probiotics, fermenting the probiotics for 2 days so as to obtain probiotics fermentation liquor; placing the traditional Chinese medicines into the probiotics fermentation liquor for fermentation; and finally mixing the dried fermentation products with other raw materials so as to obtain the Chinese herbal medicine biological feed additive for the livestock. The prepared Chinese herbal medicine biological feed additive for the livestock disclosed by the invention has the advantages of improving the immunity, increasing the utilization rate of the feed, improving environment and the like.

The present invention relates to cyclic peptidomimetic compounds as therapeutic agents capable of inhibiting the programmed cell death 1 (PD1) signalling pathway. The invention also relates to derivatives of the therapeutic agents. The invention also encompasses the use of the said therapeutic agents and derivatives for treatment of disorders via immunopotentiation comprising inhibition of immunosuppressive signal induced due to PD-1, PD-L1, or PD-L2 and therapies using them.

Disclosed is a method to recover an antigen presenting cells (APCs) and immune cells rich mixture (AIM) from peripheral blood mononuclear cells (PBMC) mobilized with one or more cell adhesion inhibitors for the preparation of an AIM vaccine or an AIM adoptive immunotherapy preparation. In addition, AIM mobilization can be enhanced by priming, simultaneously or in sequence, one or more of a combination of different chemical compounds, cytokines, hormones, growth factors, etc. The interaction of chemokines and chemokine receptors enable tumor cells attachment or in close proximity to antigen presenting cells and immune cells which possess similar receptors in a micro niche environment. Severing the chemokine/chemokine receptor linkage by a cell adhesion inhibitor will release these specifically primed cell mixtures into the peripheral blood. The collection of these cells from the peripheral blood has never been described and is the basis of this invention. AIM cells can either be used alone or better still, be induced into more target specific preparations with additions, modifications and incubation, pre or post cell adhesion inhibitors mobilization, with vaccines, different target specific antigens, peptides, chemotherapeutic agents, oncolytic viral therapeutic agents, cytokines, co-stimulatory molecules, anti-regulatory T cell therapeutic agents, anti-CTLA4, anti-PD1 molecules and other methodologies of immunological enhancement known to the art. The AIM vaccine or AIM adoptive immunotherapy preparation can then be used, but not limited to, the treatment of cancer and other diseases.

The present invention provides an immunogenic composition comprising a DNA expression vector encoding both an immunopotentiating chemokine sequence as well as an immunogenic polypeptide sequence. Immunogenic polypeptide sequences are those of infectious agents or of cancerous cells. Also provided are methods of manufacturing various immunogenic compositions, and methods of using such compositions to treat cancer and infectious disease.

The invention provides a nibea albiflora richardson larvae and juvenile microcapsule feed which is prepared from white fish meal, euphausiid meal, squid meal, fish protein hydrolysate, feed yeast, alpha-starch, soybean lecithin, papain, fish oil, composite vitamin, composite mineral substance, binder, immunopotentiator, phagostimulant and the like. By adopting the innovated prescription and processing process, the microcapsule feed can fulfill the requirement for nutrition of larvae and juvenile growth, has the advantages of comprehensive nutrition, good palatability, high water stability, low cost and the like, can be produced in batches, is convenient for storing, transporting and feeding, is suitable for industrial breeding production, and adopts a corresponding feeding method, so that the survival rate of nibea albiflora richardson is greatly improved, the shape of the nibea albiflora richardson is increase. The nibea albiflora richardson larvae and juvenile microcapsule feed is suitable for large-scale popularization and application.

The invention discloses a water-in-oil-in-water adjuvant vaccine and a preparation method thereof. The adjuvant vaccine is composed of an internal water phase, a middle oil phase used for cladding the internal water phase, and an external water phase for cladding the middle oil phase. The external water phase comprises: a composite surfactant, a hydrophilic surfactant and mormal saline. The middle oil phase consists of: a lipophilic surfactant, a stabilizer and mineral oil. The internal water phase comprises: an immunopotentiator solution, the hydrophilic surfactant, and an inactivated antigen solution. The W/O/W (water-in-oil-in-water) type adjuvant vaccine provided in the invention can induce an immunoreaction earlier than traditional W/O (water-in-oil) type adjuvant vaccines, and can make experimental animals obtain earlier protective antibodies. The vaccine has good absorption, and causes a small inflammatory response at an inoculation site. The adjuvant vaccine disclosed in the invention has better stability than existing W/O/W type vaccines, and the antibody generation speed is fast.

The invention discloses a fermented Chinese herbal immunopotentiator used for snakeheads. With the efficacy of prevention, health care, treatment and immunoregulation, the Chinese herbal immunopotentiator is an ideal, green, environmental friendly, safe and novel feed additive for substituting antibiotic drugs. The fermented Chinese herbal feed additive is prepared by subjecting compound Chinese herbal medicines to a process of ''ultrasonic wave assisted extraction of Chinese herbal medicines and multi-strain two-step fermentation'', has the advantages of both Chinese herbal medicines and probiotics, can efficiently condition the intestinal health of snakeheads, treat both symptoms and root causes, consolidate the basis and foster vitality, maintain intestinal microecological balance and an optimal immune state, improves the digestibility of nutrients, improves the body immunity of snakeheads, improves farming profits, and reduces farming risk. The Chinese herbal immunopotentiator can be used for replacing antibiotics, has no adverse effect on snakeheads, is safe to use, and is free of drug residue, drug resistance and other problems, thus having broad market prospects.

The invention provides veterinary compound acanthopanax granules with immunopotentiation, which is prepared from the following Chinese medicament raw materials by weight percentage: 70 to 60 percent of acanthopanax and the balance of astragalus root, wherein 100 grams of granules contain 100 grams of crud drugs. The compound acanthopanax granules produced by the method have good stability and safety, are mainly applied to strengthening the immunological effect of a vaccine and strengthening the immunity of organisms and the anti-stress efficacy clinically, and is applied to treating various immunosuppressive diseases of chickens and other animals and birds, strengthening the immunological effect, and treating symptoms of hypoimmunity, inappetence and the like caused by transportations, groups changes, abrupt climate changes and the like.

The invention provides an immunity enhancing agent, an inactivated vaccine, and a preparation method thereof. The invention relates to the field of biopharmaceutical. The immunity enhancing agent comprises 0.1-21mg/mL of monophosphoryl lipid A, 1.5-125mg/mL of muramyl dipeptide, and 0.7-4.5mg/mL of beta-glucan. The invention also provides the inactivated vaccine comprising the immunity enhancing agent, and a preparation method of the inactivated vaccine. According to the invention, the immunity enhancing agent is mixed with an inactivated antigen solution, such that a water-phase solution is obtained; and the water-phase solution is mixed with an oil-phase solution, such that the inactivated vaccine is obtained. According to the immunity enhancing agent provided by the invention, with a synergetic effect of the components, body immunity level can be improved, and immune response to antigen can be improved, such that antibody level after immunization can be increased, immune window period can be shortened, and vaccine immunization effect can be enhanced. According to the inactivated vaccine comprising the immunity enhancing agent, antibody level after immunization is high, a protection period is long, and immunization window period is short.

The invention relates to a whole Luchuan pig feed capable of improving Luchuan pig feeding rewards. The whole Luchuan pig feed aims at overcoming the defects of short and small bodies, low growth speed and low feeding rewards of Luchuan pigs and can promote the growth and development and improve the immunity and digestion and absorption functions as well as infection resistance by improving nutritional components of the feed. The feed comprises the following raw materials: bean dregs, corn flour, potato powder, wheat middling, wheat flour, sorghum flour, rice flour, wheat bran, paddy meal, rice bran, calcium hydrophosphate, table salt, lysine and a premix, wherein the premix comprises living lactic acid bacteria, yeast autolysate, chelate metal trace elements, active calcium and phosphorus, fermentation type traditional Chinese medicine, complex vitamins, an immunopotentiator, mannan oligosaccharide and xylooligosaccharide. The cost unit price of the feed is about half of that of a traditional complete feed on the market, so that the breeding cost of pigs is lowered; the pigs can be slaughtered 15 days in advance, pig houses are free of odor, the pigs are healthy, and are low in morbidity, high in survival rate and good in meat quality and flavor, and the medical expense is reduced.

The invention discloses crab fodder with added enteromorpha prolifera and a Chinese herbal medicine immunopotentiator. The fodder product comprises raw materials of 15-20 parts of bran, 20-30 parts of smashed straw, 10-15 parts of wheat shorts, 8-12 parts of enteromorpha prolifera powder, 8-10 parts of bean pulp, 8-10 parts of cottonseed meal, 12-18 parts of high protein flour, 4-6 parts of dried blood, 3-5 parts of sea fish oil, 4-6 parts of animal fat, 1-3 parts of inkfish paste, 0.5-1 part of Chinese herbal medicine mixture, 0.5-1.5 parts of choline chloride, 0.3 part of salt, 0.2 part of glycine betaine, 0.2 part of inositol, 0.5 part of multi-vitamin, 1 part of composite microelement and 0.5 part of allicin. The product has the advantages that crabs are promoted to grow quickly obviously, the cultivation breeding rate is improved, cultivation cost is lowered, the breeding digestibility is improved, nitrogen and phosphorus excretion is lowered, and breeding environment is improved, and high cost performance and promotion application value are achieved.

The invention discloses a Chinese herbal medicine immunopotentiator for resisting white spot syndrome virus (WSSV) of procambarus clarkia, as well as a preparation method and an application thereof. The preparation method comprises the following steps: milling rheum officinale, epimedium, astragalus membranaceus and isatis root and fully mixing the ingredients in proportion to prepare the powdery immunopotentiator. The Chinese herbal medicine immunopotentiator disclosed by the invention is stable and wide in raw material resources, simple to prepare, and low in cost, is based on the immunomechanism of shellfish to provide Chinese herbal medicines for the shellfish, and can be used for improving the immunity and oxidation resistance of a body during application, improving the capacity of the procambarus clarkia for resisting WSSV infection, and promoting the growth of the procambarus clarkia.

The invention relates to an invitro recombinant expression technology and functional identification of chlamys farreri peptidoglycan recognition protein (Cf-PGRP) gene. Cf-PGRP is provided with an amino acid sequence in a SEQ ID NO.1. The Cf-PGRP is obtained by the invitro recombinant expression technology, and the recombinant protein has broad sterilizing effect, obvious killing effect on Gram positive bacteria and the certain antibacterial effect on Gram negative bacteria and potential application value in aspects of developing broad antibacterial medicines, immune reinforcing agent, feed additive, and the like.

The invention discloses loach feed with enteromorpha prolifera and a Chinese herbal medicine immunopotentiator. The loach feed comprises 15-20 parts of crushed straw, 20-30 parts of corn flour, 10-15 parts of vinasse, 8-12 parts of enteromorpha prolifera powder, 8-10 parts of soybean meal, 8-10 parts of sesame meal, 12-18 parts of high protein flour, 4-6 parts of fish meal, 3-5 parts of sea fish oil, 4-6 parts of animal grease, 1-3 parts of squid paste, 0.5-1 part of a Chinese herbal medicine mixture, 0.5-1.5 parts of choline chloride, 0.3 parts of table salt, 0.2 parts of betaine, 0.2 parts of inositol, 0.5 parts of composite vitamins, 1 part of composite trace elements and 0.5 parts of allicin. The loach feed can observably promote loach rapid growth, improve a culture survival rate, reduce a culture cost, improve culture digestion and absorption rates, reduce nitrogen and phosphor excretion and improve a culture environment and has high cost performance and a high popularization application value.

The invention discloses a fermentation type Chinese herbal medicine immunoenhancer used for channel catfish. The fermentation type Chinese herbal medicine immunoenhancer used for the channel catfish has multiple effects of prevention, healthcare, treatment, immune adjustment and the like, and is an ideal green environmental-friendly safe novel feed additive capable of replacing antibiotics. The fermentation type Chinese herbal medicine additive is prepared from compound Chinese herbal medicines through the processes of ultrasonic-assisted extraction of the Chinese herbal medicines, as well as aerobic fermentation and anaerobic fermentation combined secondary fermentation of multi-strains, has the double advantages of the Chinese herbal medicines and probiotics, efficiently adjusts the intestinal health of the channel catfish, treats both manifestation and root cause of diseases, consolidates basis, keeps microecological balance of the intestinal tract and the best immune state, improves the digestion and absorption rates of nutrient substances, improves the immunity of the channel catfish and the cultivation benefit, reduces the cultivation risk, replaces the antibiotics, has no bad influences on the channel catfish, is safe to use, does not have the problems of drug residues, tolerance and the like, and has a wide market prospect.