417 results about "Immunopotentiation" patented technology

The present invention relates to an immunomicrobubble complex and its uses. The immunomicrobubble complex (IMC) according to the present invention comprises microbubbles conjugated with an antibody, wherein the microbubbles have excellent stability and excellent antibody binding strength, and it has been confirmed that when the immunomicrobubble complex is treated with high-intensity focused ultrasound (HIFU), the anti-tumor effect is significantly enhanced and an immune-enhancing effect is exhibited. Therefore, the immunomicrobubble complex according to the present invention is expected to improve the delivery efficiency of the conjugated antibody and can be used for the diagnosis and treatment of cancer, and exhibits various functions in the field of immunotherapy, including a contrast effect, improvement of half-life, improvement of drug delivery, lymphocyte concentrating effect, cancer immunotherapy, and ultrasound-induced immunotherapy.

The invention provides a microecological preparation with an immunological enhancement function and application of the microecological preparation in antibiotic-free breeding of livestock and poultry, and relates to the technical field of agricultural biology. The microecological preparation with the immunological enhancement function comprises a probiotic component, a prebiotics component and an immunological enhancement component, and the probiotic component, the prebiotics component and the immunological enhancement component are respectively composed of lactic acid bacteria, bacillus, clostridium butyricum, fructo-oligosaccharide, yeast beta-glucan, astragalus polysaccharide and chitosan oligosaccharide. Wherein the lactic acid bacteria accounts for 30%-40%, the bacillus accounts for 20%-30%, the clostridium butyricum accounts for 10%-20%, the fructo-oligosaccharide accounts for 5%-10%, the yeast beta-glucan accounts for 3%-8%, the astragalus polysaccharide accounts for 2%-5%, and the chitosan The probiotics can enhance immunity and regulate intestinal tracts at the same time, the effect is improved by 40% or above compared with common probiotics, animals suffer less illness and grow fast, flora can resist high temperature and gastric acid through a special protection technology, the loss of viable bacteria is less than 5% after the probiotics are stored for 3 months, and the probiotics are stable by two times compared with conventional products.

The invention belongs to the technical field of biological medicine, and particularly discloses a composite vaccine adjuvant as well as a preparation method and application thereof. The composite vaccine adjuvant provided by the invention is an oil-in-water emulsion, and simultaneously contains squalene, polysorbate 80, sorbitan trioleate, polyinosinic acid (Poly I: C) and CpG oligodeoxynucleotide (CpG-ODN). The sequence-optimized CpG-ODN and Poly I: C are used in a specific emulsion system in a combined mode, a remarkable synergistic immune enhancement effect can be generated, the induced humoral immunity and cellular immunity response level is remarkably higher than the sum of the effects when all the components are independently used, and the composite adjuvant is good in stability, high in immunostimulatory activity and suitable for clinical application. The immune effect can be remarkably improved by combined application with various vaccine antigens. The invention also provides a preparation method and application of the composite vaccine adjuvant.

The invention discloses a feed additive for improving animal immunity and a preparation method, and belongs to the technical field of animal feed. The nutrient components comprise the following raw materials in parts by weight: 10-20 parts of amino acids, 0.5-3.0 parts of vitamins and 15-22 parts of minerals; the traditional Chinese medicine components comprise 20-30 parts of radix astragali, 15-25 parts of radix codonopsis, 5-20 parts of rhizoma atractylodis macrocephalae, 10-20 parts of poria cocos, 10-15 parts of radix isatidis, 5-15 parts of honeysuckle flowers, 6-12 parts of echinacea purpurea, 3-12 parts of radix acanthopanacis senticosi and 3-8 parts of fiveleaf gynostemma herb; functional additives: 3-5 parts of probiotics, 2-4 parts of an enzyme preparation and 1-3 parts of an antioxidant. According to the invention, a multi-dimensional synergistic effect is achieved, and a traditional Chinese medicine immune enhancement-antiviral core formula system is formed; through cooperation with nutrients and functional additives, a composite protection system of immunity enhancement, nutrition optimization, intestinal health and stress resistance is constructed. The immunity and the disease resistance of animals are obviously improved.

A composition includes postbiotics comprising two or more of the following components: Lactobacillus acidophilus, Lactobacillus rhamnosus, and Saccharomyces boulardii; wherein the postbiotics are dead, whole-cell postbiotics. A method of providing to a subject an immunomodulatory, immune regulatory and / or immune boosting effect through administering the composition is also provided. A method of making the postbiotic composition is also provided.

The invention belongs to the technical field of biological medicines, and particularly relates to application of bleomycin or bleomycin analogues in preparation of an immunopotentiator. The invention also provides a pharmaceutical composition and application of the pharmaceutical composition in preparation of drugs for treating and / or preventing cancers. The pharmaceutical composition comprises bleomycin, bleomycin analogues or a combination thereof and an anti-tumor immunotherapeutic agent.

The invention relates to the technical field of biological pharmacy, and discloses a QS-21 saponin adjuvant as well as a preparation method and application thereof, the adjuvant is a composite liposome and comprises a liposome skeleton composed of distearoyl phosphatidylcholine and cholesterol, and QS-21 saponin, monophosphoryl lipid A and a local anesthetic are jointly entrapped in the liposome skeleton. The problem that high reactogenicity and immunogenicity of potent adjuvants are difficult to consider at the same time is solved, the immunostimulation component and the pain inhibition component are jointly entrapped in the same nano-carrier, collaborative delivery in injection local is achieved, and therefore pain and swelling caused by the adjuvants are accurately inhibited. The co-entrapment structure avoids the potential inhibition effect of the free anesthetic on the immune system, the potent body fluid and cellular immune enhancement activity of the adjuvant is completely reserved, and a new technical scheme is provided for developing vaccines with high safety and strong immune efficacy.

The invention discloses allium fistulosum and bolete polysaccharide as well as a preparation method and application thereof. The allium fistulosum and bolete polysaccharide is a glycopolymer composed of arabinose, xylose, mannose, glucose and galactose. The allium fistulosum bolete polysaccharide disclosed by the invention has strong antioxidant capacity, has a stimulation and activation effect on macrophage immune response, has an inhibition effect on alpha-glucosidase, has the effects of resisting oxidation, enhancing immunity and reducing blood sugar, and can be applied to development of health-care foods and functional foods.

The invention discloses a manganese-enriched albumin boron-containing nano preparation as well as a preparation method and application thereof, and belongs to the field of medicines and nano biological materials. According to the manganese-enriched albumin boron-containing nano preparation, albumin serves as a core carrier, phenylboronic acid derivatives are loaded through covalent or electrostatic interaction, then a manganese oxide layer is deposited on the surface in situ, and the prepared manganese-enriched albumin boron-containing nano preparation can remarkably increase the boron accumulation amount in tumor tissue; local and systemic immune response of tumors is promoted through the immune activation effect of manganese ions, so that the treatment effect of boron neutron capture therapy (BNCT) is remarkably improved. According to the invention, a phenylboronic acid derivative with tumor targeting is compounded with albumin, and manganese oxide is deposited in situ on the surface of the phenylboronic acid derivative, so that a multifunctional nano preparation with high boron delivery efficiency, immunological enhancement and magnetic resonance imaging functions is formed.

The invention relates to the technical field of aquaculture fish vaccines, in particular to a fusion gene of grass carp NPM1a and II type reovirus VP38, recombinant protein, a composition and application. The nucleotide sequence of the fusion gene is as shown in SEQ ID NO: 1. The fusion gene is obtained by connecting grass carp NPM1a and II type reovirus VP38 through a linker sequence, a BamH restriction enzyme cutting site is added to the 5'end of the fusion sequence, and an XhoI restriction enzyme cutting site is added to the 3 'end of the fusion sequence; the sequence of the linker is as shown in SEQ ID NO: 2. The subunit vaccine of the fusion protein of the grass carp NPM1a and the II type reovirus VP38 is prepared, and indole-3-lactic acid is used as an immunopotentiator in a combined manner, so that the technical bottlenecks of low protection rate, poor intestinal mucosal barrier repairability and the like of the existing vaccine are overcome.

The invention provides an influenza vaccine adjuvant based on lentinan as well as a preparation method and application thereof. The active ingredient of the vaccine adjuvant is lentinan, the lentinan is extracted from fresh lentinus edodes through a water extraction and alcohol precipitation method, no additional chemical modification is needed, the vaccine adjuvant can be used for preparing the influenza virus vaccine, and the influenza virus vaccine at least contains one of three inactivated influenza virus antigens, namely H1N1 A, H3N2 A and Victoria B. The invention also provides a vaccine preparation and a preparation method thereof, the vaccine preparation comprises the vaccine adjuvant and an influenza virus vaccine, the content of lentinan in the vaccine preparation is 4 mg / mL, and the vaccine preparation has an immunological enhancement effect.

The invention discloses a QS-21 composite adjuvant and a preparation method thereof, and belongs to the technical field of biological medicines. According to the QS-21 composite adjuvant, a polyanion material and QS-21 are compounded to form nanoparticles, and the nanoparticles are organically combined with a freeze-drying protective additive, so that the prepared QS-21 composite adjuvant is relatively high in retention rate, the problems of hydrolysis and oxidation are solved, the requirement of a phospholipid carrier is avoided, and a vaccine co-preparation process is simplified; the subsequently added freeze-drying protective agent retains the immune enhancement activity of the QS-21 and reduces the exposure of the QS-21 to a degradation environment; the corresponding cytotoxicity meets the standard, and the hemolysis rate is low; therefore, the method has remarkable clinical application value and industrialization potential.

The invention discloses a dendrobium huoshanense polysaccharide nanoemulsion immunologic adjuvant and a preparation method thereof. The dendrobium huoshanense polysaccharide nanoemulsion immunologic adjuvant is prepared from the following raw materials in percentage by mass: 0.1%-2.0% of dendrobium huoshanense polysaccharide, 20%-30% of a surfactant, 5%-10% of a cosurfactant, 5%-10% of an oil phase and the balance of deionized water. The preparation method of the nano-emulsion is simple and easy to implement, equipment is easy to control, the cost is low, and the obtained nano-emulsion is small in droplet particle size, high in stability and capable of improving the spleen lymphocyte proliferation activity of immunocompromised mice. The nanoemulsion can significantly improve the immunomodulatory activity of dendrobium huoshanense polysaccharide, and has the potential of developing immunopotentiators, thereby promoting the development and utilization of traditional Chinese medicine resources and active components thereof.

The invention belongs to the technical field of animal immunopotentiators, and particularly relates to an oil-in-water type veterinary adjuvant and a preparation method thereof. The oil-in-water type veterinary adjuvant comprises white oil, span 80, Tween 80, PEG400, astragalus polysaccharide and a phosphate buffer solution, the astragalus polysaccharide cooperates with a surfactant to activate a TLR4 / NF-kappa B pathway, Th1 / Th2 / Th17 three-way immune response is achieved, the serum IgG and mucous membrane sIgA antibody level is remarkably improved, and the problems that a traditional O / W adjuvant is high in layering rate (smaller than 5%), and the stability of the traditional O / W adjuvant is poor are solved through compound optimization of the HLB value (HLB = 11.5-12.0) of the HLB value of span 80 and Tween 80. And storage at 4 DEG C for 90 days) and poor freeze-thaw stability are solved.

The invention discloses an enzymolysis preparation method of high-immunocompetence bovine coloctrum peptide, active peptide and application thereof, and belongs to the field of biotechnology and functional foods.The enzymolysis preparation method is characterized by comprising the steps that bovine coloctrum is degreased, trehalose is added into obtained supernate, first stirring is conducted, and first mixed liquid is obtained; wherein the supernate comprises whey protein and Ig; adding medium protease into the first mixed solution for hydrolysis to obtain enzymatic hydrolysate; wherein the enzymatic hydrolysate comprises a mixture of bovine colostrum hydrolyzed peptides, the Ig activity retention rate is larger than or equal to 90% through the targeted protection and directional enzymolysis technology, the beta-lactoglobulin peptide with the synergistic immune enhancement effect is obtained, the Ig and small molecule peptides in the product have the synergistic effect, the body immune regulation capacity and the intestinal immune function are remarkably improved, the immunity of the human body is improved, and the immunity of the human body is improved. The method can be widely applied to the fields of infant formulas, postoperative nutrition, functional drinks and the like.

The invention discloses an immunogenic composition containing an adjuvant as well as a preparation method and application of the immunogenic composition. The immunogenic composition comprises self-assembled gE virus-like nanoparticles, an immunopotentiator, namely, saponin QS-21, and a neutral liposome, the self-assembled gE virus-like nanoparticles are formed by polymerizing and assembling monomers; the monomers include VZV gE, a linker peptide (SGS), and a VZV gI polypeptide containing a Th epitope. The immunogenic composition can be applied to varicella-zoster virus vaccines, and solves the technical problems of weak gE immunogenicity and serious vaccine side reaction in vaccines prepared in the prior art. The immunogenicity of the vaccine is equivalent to that of the Xinanlii, and the use of an immunopotentiator in the vaccine can be reduced, so that the clinical side reaction of the vaccine is lower; in addition, the vaccine can induce a gI specific antibody and gI specific CMI reaction, and the effectiveness of the vaccine can be further improved. In short, the immunogenic composition has good clinical application potential.

The invention belongs to the field of biological medicine and ultrasonic medicine, and provides a barium titanate (BTO) piezoelectric material-based ultrasonic responsive targeting nano system (BTO at BAL), which is used for matrix microenvironment remodeling and immunopotentiation of compact tumors such as pancreatic cancer and the like. According to the nanoparticles, oleic acid modified BTO is taken as a core, ROS responsive prodrug molecules containing a small molecule PD-L1 inhibitor BMS1166 and a nitric oxide donor (Arg) 9 are loaded on the surface, and specific targeting on pancreatic cancer cells is realized by combining a targeting peptide LFC131. Under ultrasonic excitation, BTO generates reactive oxygen species (ROS), on one hand, (Arg) 9 is oxidized to release NO, tumor matrix is degraded, tumor hardness is reduced, drug delivery efficiency is improved, and meanwhile, ROS and NO synergistically enhance tumor immunogenicity; on the other hand, the ROS breaks a thioketal bond to release BMS1166, PD-L1 expression is down-regulated, and immunosuppression is reversed. According to the present invention, the significant tumor inhibition and immune activation effects are represented in the animal model, and the clinical transformation potential is provided.

The invention relates to an mRNA (messenger Ribonucleic Acid) vaccine targeting FomA and application of the mRNA vaccine in immunotherapy of esophageal squamous carcinoma in the technical field of tumor immunotherapy. The problems that in the prior art, a specific targeting vaccine for the fusobacterium nucleatum membrane protein FomA lacks, traditional antibiotics can only kill extracellular bacteria and cannot remove intracellular colonized fusobacterium nucleatum, and cellular immunity cannot be effectively activated to remove fusobacterium nucleatum colonized in tumor tissue; the immunotherapy drug resistance of esophageal squamous carcinoma caused by fusobacterium nucleatum infection is difficult to reverse. According to the technical scheme, an mRNA molecule with a fusobacterium nucleatum FomA extracellular domain tandem repeat sequence and a lipid nanoparticle delivery system are coded, an extramembrane segment tandem repeat immune enhancement technology is adopted for design, two extracellular ring sequences are repeated for 2-5 times and connected through a connector sequence, lipid nanoparticles are composed of optimized quaternary lipids, and the lipid nanoparticles are prepared from the quaternary lipids. And a PD-1 monoclonal antibody is combined to activate FomA specific CD8 + T cell immunoreaction, so that comprehensive removal of intracellular and extracellular bacteria and bacteria targeted immune remodeling are realized.

The invention relates to a fungus-diatom synergistic immunopotentiator for stichopus japonicus, and belongs to the technical field of microbial engineering and echinoderm immunology. The immunopotentiator is a symbiont microsphere taking yarrowia lipolytica and navicula seminuda as main components; the method comprises the following steps: respectively activating yarrowia lipolytica and navicula seminuda, carrying out expanding culture, resuspending, and adjusting the concentrations of a bacterial solution and algae cells; the preparation method comprises the following steps: adding a navicula seminuda concentrated solution and a yarrowia lipolytica suspension into a triangular flask containing a YPD liquid culture medium, adding selenium-containing fucoidan polysaccharide sulfate accounting for 0.4-1.2% of the total mass of microspheres, carrying out constant-temperature shake culture, filtering by using sterile gauze to obtain microspheres, and flushing by using sterile normal saline to obtain fungus-diatom symbiont microspheres; the yarrowia lipolytica, the navicula seminuda and the selenium-containing fucoidan polysaccharide sulfate in the prepared immunopotentiator are mutually beneficial and have a synergistic effect, and the non-specific immune function of the stichopus japonicus is comprehensively improved by improving the growth index, enhancing the activity of immune enzyme and digestive enzyme and improving the disease resistance.

The invention discloses neutral homogeneous lycium barbarum polysaccharide as well as a preparation method and application thereof. Through systematic structure characterization, it is clarified for the first time that the polysaccharide NLBPE1 is atypical arabinogalactan with 1, 5-alpha-L-arabinose as a bridging unit, and the polysaccharide NLBPE1 has a uniform molecular weight and a definite branched chain topological structure. The NLBPE1 serving as an adjuvant and an antigen are jointly entrapped in various delivery systems (such as PLGA nanoparticles, lipidosome, hydrogel and the like) to form the vaccine composition, so that the immunogenicity of the vaccine can be remarkably improved, and the antigen-specific T cell response can be enhanced. The invention provides an application scheme of the polysaccharide as a vaccine adjuvant in tumor immunotherapy and tumor prevention, and particularly shows a remarkable synergistic anticancer effect when the polysaccharide is combined with an immune checkpoint inhibitor for use. The polysaccharide has a clear structure, good biocompatibility and remarkable immunological enhancement activity, and has a wide application prospect of tumor vaccine adjuvants.

The invention provides a bovine colostrum probiotic compound preparation as well as a preparation method and application thereof. The compound preparation provided by the invention comprises the bovine colostrum powder subjected to activity protection and the compound probiotics, and the bovine colostrum powder subjected to activity protection is obtained by adopting a microcapsule double-layer embedding and freeze-drying technology, so that the gastric acid retention rate (2h) of immune globulin in the bovine colostrum powder is greater than or equal to 90%, and IgG contained in the bovine colostrum powder is ensured to be efficiently delivered to intestinal tracts to play an immune regulation role. Wherein the compound probiotics comprise four stages of functional echeles formed by more than or equal to 6 strains, and the four stages of functional echeles comprise colon colonization major flora, gastric acid-resistant pioneer flora, antibacterial peptide secretion auxiliary flora and inflammation regulation backup flora. Through cooperation of the components in the compound preparation, the compound preparation has excellent immune enhancement and metabolic regulation functions.

The invention discloses mucosal immune-enhanced recombinant lactobacillus for expressing PEDV (porcine epidemic diarrhea virus) S1 protein as well as a construction method and application of the mucosal immune-enhanced recombinant lactobacillus. The mucosal immune-enhanced recombinant lactobacillus contains a recombinant lactobacillus expression vector for expressing PEDV S1 protein, the amino acid of the PEDV S1 protein is fused with M cell targeting peptide (Co1) and dendritic cell targeting peptide (6aa), the carboxyl terminal of the PEDV S1 protein is fused with a mucosal immune adjuvant (LTB), and the connection sequence of all the parts is Co1-6aa-S1-LTB. According to the mucosal immune enhanced recombinant lactobacillus constructed by the invention, through the synergistic effect of the targeting peptide (DC / MC targeting peptide) and the adjuvant (LTB), the mucosal immune response efficiency (including intestinal mucus SIgA and serum IgG levels) of a PEDV S1 antigen is remarkably improved, humoral immunity, cellular immunity and mucosal immune responses of pregnant animals can be remarkably induced, maternal antibodies are generated, and the immune response efficiency of the pregnant animals is remarkably improved. The intestinal SIgA level of newborn animals is improved, and an effective technical means is provided for prevention and treatment of PED.

The invention is applicable to the technical field of genetic engineering and biological medicine, and provides construction of a fusion recombinant protein ABT for preventing brucellosis and application of the fusion recombinant protein ABT in preparation of protective vaccines. The fusion recombinant protein ABT is composed of an immunological enhancement antigen and a multi-epitope tandem antigen, wherein the immunological enhancement antigen (named as A) comprises a Brucella ribosome L7 / L12 protein and a PADRE sequence; the multi-epitope tandem antigen is formed by connecting a dominant B cell epitope part (named as B), a dominant Tc cell epitope part and a dominant Th cell epitope part (named as T) in series, wherein the dominant B cell epitope part (named as B) is derived from Brucella SurA, OMP31, BP26 and Trigger factor proteins. The ABT Brucella multi-epitope subunit vaccine prepared by mixing the fusion recombinant protein ABT and an immunologic adjuvant has the characteristics of good purity, high safety and strong immunogenicity, can stimulate an organism to generate a protective antibody, and has long antibody maintenance time.

The invention discloses an immunogenic composition containing a composite adjuvant as well as a preparation method and application of the immunogenic composition. The immunogenic composition comprises a gE-gI fusion protein, an immunopotentiator, namely, saponin QS-21, and a neutral liposome, the gE-gI fusion protein comprises a VZV gE, a connecting peptide and a VZV gI. The immunogenic composition provided by the invention only contains one immunopotentiator, can be applied to VZV vaccines, and solves the problems of relatively weak gE immunogenicity and relatively large clinical side reaction of the vaccines prepared in the prior art. The immunogenicity of the vaccine is equivalent to that of the Xinanlii, but the vaccine contains fewer immunopotentiators and has lower clinical side effects; in addition, the vaccine can also induce a gI specific antibody and gI specific CMI reaction, which is beneficial to further improvement of the effectiveness of the vaccine. In short, the immunogenic composition has good clinical application potential.

The invention belongs to the technical field of medicine and health care, and discloses oleander oligosaccharide and a preparation method and application thereof, the molecular weight of the oleander oligosaccharide is 2900 Da, and the oleander oligosaccharide is composed of glucose, mannose, galactose and arabinose; the preparation method comprises the following steps: degreasing oleander roots with ethanol, respectively carrying out water extraction and gradient concentration alcohol extraction, and then sequentially carrying out ceramic membrane-ultrafiltration-nanofiltration enrichment, ion exchange column chromatography and molecular sieve gel column purification on the alcohol extract to obtain the oleander extract. The oligosaccharide can be applied to preparation of immunological enhancement drugs, corresponding tumor immune drugs or health care products. The preparation method is efficient and safe, and the prepared product is good in quality and can be applied to preparation of immunological enhancement drugs, corresponding tumor immune drugs or health care products.

The present invention relates to a composition for enhancing immunity, containing, as an active ingredient, a polysaccharide enzyme-treated product derived from a mixed extract of Angelica gigas, Cnidium officinale, and Paeonia lactiflora, or a novel purified polysaccharide fraction thereof. The composition for enhancing immunity according to the present invention, comprising the polysaccharide enzyme-treated product of the mixed extract of Angelica gigas, Cnidium officinale, and Paeonia lactiflora or the novel purified polysaccharide fraction thereof, is composed of specific types and contents of sugars and significantly increases the activity of macrophages, splenocytes, and NK cells upon application thereto, thereby being applicable as an immunopotentiator or a composition for enhancing immunity.

This application belongs to the field of biology and discloses a strain of Candida tropicalis, which is classified and named candida tropicalis The deposit number is GDMCC NO: 65030; the deposit date is August 20, 2024, and it is deposited at the Guangdong Provincial Center for Microbiological Culture Collection, with the deposit address being: 5th Floor, Building 59, No. 100, Xianlie Middle Road, Guangzhou. When fermented with this tropical yeast, Astragalus polysaccharide can enhance the immune-enhancing effect of Astragalus polysaccharide, stimulate animals to produce higher antibody titers after immunization, and reduce adverse immune reactions. The present invention also provides an Astragalus polysaccharide fermentation product, a preparation method, and applications.

The application provides a momordica root polysaccharide and a preparation method and application thereof, and belongs to the technical field of natural product development. The preparation method of the momordica root polysaccharide comprises the following steps: drying and crushing momordica roots, and removing fat by ethanol reflux; after water extraction and alcohol precipitation, and removing protein, a momordica root crude polysaccharide is obtained; and the momordica root crude polysaccharide is sequentially separated and purified by DEAE-52 cellulose column and Sephadex LH20 gel column to obtain the momordica root refined polysaccharide. The preparation method has the advantages that the polysaccharide obtained by directly extracting water in the prior art has a narrow molecular weight distribution range and better immune enhancement activity. Experimental results show that the momordica root polysaccharide can stimulate RAW264.7 cells to secrete NO, TNF-alpha and IL-6 factors, enhance the phagocytosis of RAW264.7 cells, and thus enhance the cell immune function, and can be applied to the development of products for improving immunity.

The application discloses a preparation method of an immune protein extract of colostrum with tumor inhibition efficacy, and comprises the following steps: (1) raw material selection; (2) flocculation of casein; (3) sugar removal; (4) ultrafiltration membrane filtration; (5) directional hydrolysis; (6) cation exchange resin adsorption; (7) desalination; and (8) freeze drying. The extract of the application is rich in hydrophobic amino acid and alkaline amino acid hydrolysis fragments of the immune protein of colostrum, and has stronger antioxidant, immune enhancement and tumor inhibition activities. Different from common immune proteins of colostrum, the extract plays the anti-tumor activity as a result of the combined action of its double effects of immune enhancement and tumor cell growth inhibition.