The invention discloses a conditioning medicine aiming at pain and rheumatism and a preparation method of the conditioning medicine. The conditioning medicine consists of the following components in percentage by weight: 20-40 percent of coriolus versicolor mycelium polysaccharide, 15-30 percent of needle mushroom mycelium polysaccharide, 20-35 percent of polygahatous polysaccharide and 15-35 percent of rheum officinale polysaccharide. The preparation method comprises the following steps: respectively extracting the coriolus versicolor mycelium polysaccharide, needle mushroom mycelium polysaccharide, polygahatous polysaccharide and rheum officinale polysaccharide, and preparing according to the ratio. The conditioning medicine disclosed by the invention is low in cost and does not have any toxic or side effect, can achieve the effects of improving the human immunologic function, relieving swelling and pain, diminishing inflammation, expelling wind and eliminating dampness and diminishing inflammation and relieving pain and has obvious effects on traumatic injury, lumbar muscle degeneration, sciatica, prosopalgia, facial palsy and spasticity, rheumatoid arthritis, antitumor immunological therapy, internal and external hemorrhoids and colitis. The conditioning medicine is taken by adults 2-3 times per day, 1-2 grains of the medicine are taken each time, and the medicine can be taken for a long time.