The present disclosure relates to compositions comprising a recombinant influenza
neuraminidase tetramer comprising four copies of a
fusion protein comprising a DA4B tetramerization domain, an optional
linker, and an influenza NA head domain, and uses thereof. A novel
linker is provided. The present disclosure also relates to a recombinant influenza
neuraminidase (NA) polypeptide comprising an influenza NA head domain comprising one or more
amino acid substitutions selected from the group consisting of: I99P, Y100L, T131Q, C161V, E165S, S172A, V177I, S196T, V205I, Q408M, R419V, and V453T. Also provided are compositions, methods of making, and methods of use, for example, for use as influenza vaccines.