286 results about "Tropism" patented technology

Provided herein are Cocal vesiculovirus envelope pseudotyped retroviral vectors that exhibit high titers, broad species and cell-type tropism, and improved serum stability. Disclosed Cocal vesiculovirus envelope pseudotyped retroviral vectors may be suitably employed for gene therapy applications and, in particular, for the ex vivo and in vivo delivery of a gene of interest to a wide variety of target cells.

A method for studying scaffold-based tissue engineering approaches in combination with the use of progenitor or stem cells to generate new lung tissue in an in vitro system. The engineered tissue system of this invention is used to monitor lung and immune system exposure of pathogen and/or toxins. The method involves growing engineered lung/immune tissue from progenitor cells in a bioreactor and then exposing the engineered lung/immune tissue to a pathogen and/or toxin. Once exposed, response of the engineered tissue is monitored to determine the effects of exposure to the immune component of the tissue and to lung component of the tissue. This invention also involves development of mixed engineered tissues including a first fully functional engineered tissue such as lung tissue and a second fully functional engineered tissued such as immune tissue from a single animal donor. The mixed systems can include more than two engineered tissues.

The invention provides methods and compositions for targeting a separately generated immune response to a specific organ or tissue, e.g. one affected by cancer, using one or more agents with a tropism for the organ or tissue or that can be specifically localized to the desired organ or tissue. For example, the invention provides methods ands compositions for treating liver metastases from colorectal cancer using a combination of a granulocyte/macrophage colony stimulating factor (GM-CSF) augmented tumor cell vaccination and Listeria monocytogenes (LM) infection.

The present invention provides methods of achieving directed evolution of viruses by in vivo screening or “panning” to identify viruses comprising scrambled AAV capsids having characteristics of interest, e.g., tropism profile and/or neutralization profile (e.g., ability to evade neutralizing antibodies). The invention also provides scrambled AAV capsids and virus particles comprising the same.

The invention provides a pest-attracting plate, a preparation method and application thereof, belonging to the field of plant protection. In the pest-attracting plate, pest gum and pest-attracting substances are coated on a carrier plate, wherein the pest-attracting substances are mixtures of pest attractant and dilacor which can be plant oil, honey, wax oil, glycerin, Vaseline and/or animal oil. Pests can be completely killed based on a tropism principle of pests to colour and smell with many killing types. The pest-attracting plate has durative and stable efficacy and low production cost, and is particularly suitable for promotion and popularization.

The invention relates to a display system for comparing and observing the growth of plant root systems under different growing conditions, particularly to a display system which utilizes water culture method for parallel cultivation, can satisfy continuous growth requirements of plants and is used for observing growth comparison experiments of plant root systems. The display system comprises three main parts which are a cultivation tank, a nutrient fluid circulation and regulation device and a movable stand; the cultivation tank and the nutrient fluid circulation and regulation device are respectively arranged on the movable stand and are communicated through a pipeline; and nutrient fluid required by the cultivation tank is supplied by the nutrient fluid circulation and regulation device. The display system provided by the invention can not only be used for teaching works such as comparing and displaying geotropism, nutrient-tropism, hydrotropism and other growth characteristics of plant root systems, but also provide a convenient experimental tool for continuously comparing and observing growth conditions of plant root systems under different environmental conditions, and the problems in the prior art such as incapability of measuring intuitionally and all sidedly, inaccurate simulation and observation results, lack of comparative observation function, and the like are solved.

Via recognizing the image hue info of each frame and adopting accordingly different color temperatures (CT), the background lights (BL) of different CT are adopted for different pictures to ensure more lively beautiful pictures. This CT dynamically regulation method includes steps of: (1) firstly, determining the hue tropism (HT) of single image element (IE) on one frame picture; (2) making statistic of numbers of lean-red/ lean-blue IEs of this frame picture; (3) comparing numbers of lean-red/ lean-blue IEs of this frame picture to determine HT of this frame; (4) according to the determined HT, regulating CT of BL, i.e. controlling the brightness values of the red/ blue LEDs. This offered CT dynamically regulation method ensures the vivid color of the picture and boosts the visible enjoyment. Besides, the brightness of blue light is reduced relatively when the picture appears low CT. It raises the luminescence efficiency of BL and reduces energy consumption.

The present invention relates to variant AAV capsid polypeptides, wherein the variant AAV capsid polypeptides exhibit increased transduction and/or tropism in human muscle tissue or cells as compared non-variant parent capsid polypeptides.

Adenovirus serotypes differ in their natural tropism. The adenovirus serotypes 2, 4, 5 and 7 all have a natural affiliation towards lung epithelia and other respiratory tissues. In contrast, serotypes 40 and 41 have a natural affiliation towards the gastrointestinal tract. The serotypes described differ in at least capsid proteins (penton-base, hexon), proteins responsible for cell binding (fiber protein), and proteins involved in adenovirus replication. This difference in tropism and capsid protein among serotypes has led to the many research efforts aimed at redirecting the adenovirus tropism by modification of the capsid proteins.

The invention provides a LCD device which has a high voltage retention and an restrained residual DC. Also provided is an aligning layer for liquid crystal used for producing the LCD device, an aligning agent for liquid crystal used for producing the LCD device, a polymer included in the aligning agent for liquid crystal and a new compound used for synthesizing the polymer. The invention supposes: a diamine represented by the general formula (I), a combination including one, or two or more polymers. The combination is a polymer obtained by a reaction of the tetrahydric dianhydride and the diamine represent by the general formula (I). [in the formula (I), A1 and R1 are defined as the claim].

The present invention relates to an artificially synthesized gene optiHA containing codons for chicken partial tropism. Its reading frame contains 1707 bp nucleotides and encodes a total of 568 amino acids. The gene is compatible with H5 subtype highly pathogenic avian influenza virus A/ Goose/GuangDong/1/96(H5N1)[GD/1/96(H5N1)]hemagglutinin (HA) gene has a nucleotide homology rate of 70%, an amino acid homology rate of 100%, and encodes the H5 subtype Hemagglutinin (HA) protein of avian influenza virus GD/1/96 (H5N1). The invention also relates to the application of the gene as an immunogenic gene of H5 subtype influenza DNA vaccine and other genetic engineering vaccines.

The invention discloses a microbial inoculum for improving water body and structure of biological intestinal colony, and a preparation method and application thereof. In the method, bdellovibrio bacteriovorus., photosynthetic bacteria, streptococcus lactis, Lactobacillus bulgaricus and aquaculture biological intestinal beneficial strains are mixed to obtain the microbial inoculum for improving water body and structure of biological intestinal colony. The modified microbial inoculum is applied in fresh water aquaculture process, thereby effectively improving aquaculture environment and the structure of biological intestinal colony. The tropism and splitting capability of bdellovibrio bacteriovorus are utilized in the invention, and simultaneously the vacant niche left by pathogenic bacterium can be filled by the bacteria and other good bacteria which can be colonized in the intestinal canals of cultured organisms, thereby realizing the purpose of improving aquaculture environment and the structure of biological intestinal colony. The microbial inoculum of the invention has effectiveness, environment protection and no generation of pathogenic bacteria resistance, thereby having good application prospect.

The present invention related to methods and compositions comprising recombinant vectors comprising chimeric capsids and recombinant pseudotyped vectors with non-native capsid protein(s). The recombinant vectors of the invention confer an altered tropism that permits selective targeting of desired cells.

The present invention belongs to the fiber producing method field, in particular relates to a method which uses a reclaimed PET bottle to produce the terylene preoriented filament in particular. The present invention mainly solves the problems of the prior art that the cost of raw materials for production is high, the drying efficiency is low, and the uniformity is poor, etc., and provides a method which uses the reclaimed PET bottle to produce the terylene preoriented filament with low cost of the raw materials for production, high drying efficiency, and good uniformity. The present invention has the major steps that the bottle piece of the PET bottle is produced; the bottle piece is continuously crystallized and dried by passing through a pre-crystallizer and a drying tower; the dried bottle piece directly enters a threaded rod extrusion machine, and is melted and extruded by a spinning threaded rod; the molten polyester material is continuously filtrated by a molten mass filter with a big filtering area of one level or two levels, to enter a spinning manifold; a thread line is cooled by passing through a cross air blasting, to wind to form the preoriented filament.

Novel adeno-associated virus (AAV) isolates in nucleotide and amino acid forms and uses thereof are provided. The isolates show tropism for certain target tissues, such as blood stem cells, liver, heart and joint tissue, and may be used to transduce stem cells for introduction of genes of interest into the target tissues. Discrete modified portions of the cap gene, VP1, VP2, and VP3, may be used alone or in combination in the present methods.

The present invention relates to a novel RNA picornavirus that is called Seneca Valley virus (“SVV”). The invention provides isolated SVV nucleic acids and proteins encoded by these nucleic acids. Further, the invention provides antibodies that are raised against the SVV proteins. Because SVV has the ability to selectively kill some types of tumors, the invention provides methods of using SVV and SVV polypeptides to treat cancer. Because SVV specifically targets certain tumors, the invention provides methods of using SVV nucleic acids and proteins to detect cancer. Additionally, due to the information provided by the tumor-specific mechanisms of SVV, the invention provides methods of making new oncolytic virus derivatives and of altering viruses to have tumor-specific tropisms.

The invention belongs to the new powder metallurgy process, and specifically to a special method for preparing an anisotropic SmCo based nanocrystalline rare earth permanent magnet. The invention breaks through the nano-crystal particle tropism problem of the SmCo based nanocrystalline rare earth permanent magnet nanocrystalline grain and obtains the nanocrystalline permanent magnet with obvious crystallographic anisotropism and magnetic anisotropism. The invention obtains the anisotropic nanocrystalline rare earth permanent magnet by the operations of adopting surfactant to auxiliary high-energy ball mill, orientating the pulse magnetic field, cold isostatic pressing and low-temperature pressurizing and sintering. The SmCo based nanocrystalline rare earth permanent magnet with obvious anisotropism can be obtained after sintering without any treatment.

The invention discloses methods for screening and evaluating sperm tropism and a dedicated microfluidic control chip thereof. The microfluidic control chip is composed of an upper layer structure and a lower layer structure, and the surface of the upper layer structure and/or the lower layer structure includes at least one microfluid pipeline unit; each microfluid pipeline unit comprises an entrance pool, at least one main pipeline with one end communicated with the entrance pool, an exit pool communicated with the other end of the main pipeline, and at least one branch pipeline communicated with the main pipeline. While in use, a physical or chemical gradient field is formed in the main pipeline. A sample is added from the entrance pool, freely moves in the gradient field of the main pipeline, and selectively enters the branch pipelines. Through counting sperms in the branch pipelines, the sperm tropism can be quantitatively evaluated; a fluid which is insoluble or slightly soluble in a cell culture solution is introduced, an interface of the two fluids is formed at the entrance port of each branch pipeline, movement of cells between the main pipeline and the branch pipelines can be limited, so as to realize capture and recovery of the sperms having tropism.