288 results about "Tropism" patented technology

Methods of making and using recombinant AAV vectors and virions for gene delivery to the lung are described. The recombinant AAV virions are derived from caprine AAV and bovine AAV, both of which display tropism for lung tissue.

Provided herein are Cocal vesiculovirus envelope pseudotyped retroviral vectors that exhibit high titers, broad species and cell-type tropism, and improved serum stability. Disclosed Cocal vesiculovirus envelope pseudotyped retroviral vectors may be suitably employed for gene therapy applications and, in particular, for the ex vivo and in vivo delivery of a gene of interest to a wide variety of target cells.

Adenovirus serotypes differ in their natural tropism. The adenovirus serotypes 2, 4, 5, and 7 all have a natural affiliation towards lung epithelia and other respiratory tissues. In contrast, serotypes 40 and 41 have a natural affiliation towards the gastrointestinal tract. The serotypes described, differ in at least capsid proteins (penton-base, hexon), proteins responsible for cell binding (fiber protein), and proteins involved in adenovirus replication. This difference in tropism and capsid protein among serotypes has led to the many research efforts aimed at redirecting the adenovirus tropism by modification of the capsid proteins.

A method for studying scaffold-based tissue engineering approaches in combination with the use of progenitor or stem cells to generate new lung tissue in an in vitro system. The engineered tissue system of this invention is used to monitor lung and immune system exposure of pathogen and / or toxins. The method involves growing engineered lung / immune tissue from progenitor cells in a bioreactor and then exposing the engineered lung / immune tissue to a pathogen and / or toxin. Once exposed, response of the engineered tissue is monitored to determine the effects of exposure to the immune component of the tissue and to lung component of the tissue. This invention also involves development of mixed engineered tissues including a first fully functional engineered tissue such as lung tissue and a second fully functional engineered tissued such as immune tissue from a single animal donor. The mixed systems can include more than two engineered tissues.

The present invention provides methods of achieving directed evolution of viruses by in vivo screening or “panning” to identify viruses comprising scrambled AAV capsids having characteristics of interest, e.g., tropism profile and / or neutralization profile (e.g., ability to evade neutralizing antibodies). The invention also provides scrambled AAV capsids and virus particles comprising the same.

The invention provides methods and compositions for targeting a separately generated immune response to a specific organ or tissue, e.g. one affected by cancer, using one or more agents with a tropism for the organ or tissue or that can be specifically localized to the desired organ or tissue. For example, the invention provides methods ands compositions for treating liver metastases from colorectal cancer using a combination of a granulocyte / macrophage colony stimulating factor (GM-CSF) augmented tumor cell vaccination and Listeria monocytogenes (LM) infection.

Methods of making and using recombinant AAV vectors and virions for gene delivery to the lung are described. The recombinant AAV virions are derived from caprine AAV and bovine AAV, both of which display tropism for lung tissue.

The present invention provides methods of achieving directed evolution of viruses by in vivo screening or “panning” to identify viruses comprising scrambled AAV capsids having characteristics of interest, e.g., tropism profile and / or neutralization profile (e.g., ability to evade neutralizing antibodies). The invention also provides scrambled AAV capsids and virus particles comprising the same.

The invention provides a pest-attracting plate, a preparation method and application thereof, belonging to the field of plant protection. In the pest-attracting plate, pest gum and pest-attracting substances are coated on a carrier plate, wherein the pest-attracting substances are mixtures of pest attractant and dilacor which can be plant oil, honey, wax oil, glycerin, Vaseline and / or animal oil. Pests can be completely killed based on a tropism principle of pests to colour and smell with many killing types. The pest-attracting plate has durative and stable efficacy and low production cost, and is particularly suitable for promotion and popularization.

The invention relates to a display system for comparing and observing the growth of plant root systems under different growing conditions, particularly to a display system which utilizes water culture method for parallel cultivation, can satisfy continuous growth requirements of plants and is used for observing growth comparison experiments of plant root systems. The display system comprises three main parts which are a cultivation tank, a nutrient fluid circulation and regulation device and a movable stand; the cultivation tank and the nutrient fluid circulation and regulation device are respectively arranged on the movable stand and are communicated through a pipeline; and nutrient fluid required by the cultivation tank is supplied by the nutrient fluid circulation and regulation device. The display system provided by the invention can not only be used for teaching works such as comparing and displaying geotropism, nutrient-tropism, hydrotropism and other growth characteristics of plant root systems, but also provide a convenient experimental tool for continuously comparing and observing growth conditions of plant root systems under different environmental conditions, and the problems in the prior art such as incapability of measuring intuitionally and all sidedly, inaccurate simulation and observation results, lack of comparative observation function, and the like are solved.

Via recognizing the image hue info of each frame and adopting accordingly different color temperatures (CT), the background lights (BL) of different CT are adopted for different pictures to ensure more lively beautiful pictures. This CT dynamically regulation method includes steps of: (1) firstly, determining the hue tropism (HT) of single image element (IE) on one frame picture; (2) making statistic of numbers of lean-red / lean-blue IEs of this frame picture; (3) comparing numbers of lean-red / lean-blue IEs of this frame picture to determine HT of this frame; (4) according to the determined HT, regulating CT of BL, i.e. controlling the brightness values of the red / blue LEDs. This offered CT dynamically regulation method ensures the vivid color of the picture and boosts the visible enjoyment. Besides, the brightness of blue light is reduced relatively when the picture appears low CT. It raises the luminescence efficiency of BL and reduces energy consumption.

An equipment for making abrasive particles in even distribution, array pattern and preferred orientation comprises an adsorbing releasing apparatus being composed of a plurality of acicular soft magnetic metallic sticks, an upper and a lower stators, and a plurality of windings. Each winding forms an electric circuit through a electrical source, a resistance, and a switch a plurality of blind holes being distributed on face of the blind hole feeding tropism board. Using electric current to form magnetic field for the acicular soft magnetic metallic sticks absorb or release abrasive particles plated with soft magnetic materials. The equipment is simple, convenient in operation, high in efficiency and reliability. The equipment makes abrasive particles in matrix with an ideal state in distributing density, uniformity, arrange, and tropism. Thereby, the function of the abrasive materials is fully exerted and the life of products is prolonged.

A change in viral tropism occurs in many HIV positive individuals over time and can be indicated by a shift in coreceptor use from CCR5 to CXCR4. The shift in coreceptor use to CXCR4 has been shown to correlate with increased disease progression. In patients undergoing HAART, the predominant populations of virus can be shifted back to CCR5-mediated entry after the CXCR4-specific strains have emerged. The present invention relates to a diagnostic method to monitor coreceptor use in the treatment of human immunodeficiency virus (HIV) infection. The present invention further relates to a diagnostic method applied to HIV-positive individuals undergoing HAART to monitor the suppression of CXCR4 specific strains. The diagnostic methods can be used to assist in selecting antiretroviral therapy and to improve predictions of disease prognosis over time.

The present invention relates to variant AAV capsid polypeptides, wherein the variant AAV capsid polypeptides exhibit increased transduction and / or tropism in human muscle tissue or cells as compared non-variant parent capsid polypeptides.

Adenovirus serotypes differ in their natural tropism. The adenovirus serotypes 2, 4, 5 and 7 all have a natural affiliation towards lung epithelia and other respiratory tissues. In contrast, serotypes 40 and 41 have a natural affiliation towards the gastrointestinal tract. The serotypes described differ in at least capsid proteins (penton-base, hexon), proteins responsible for cell binding (fiber protein), and proteins involved in adenovirus replication. This difference in tropism and capsid protein among serotypes has led to the many research efforts aimed at redirecting the adenovirus tropism by modification of the capsid proteins.

The invention provides a LCD device which has a high voltage retention and an restrained residual DC. Also provided is an aligning layer for liquid crystal used for producing the LCD device, an aligning agent for liquid crystal used for producing the LCD device, a polymer included in the aligning agent for liquid crystal and a new compound used for synthesizing the polymer. The invention supposes: a diamine represented by the general formula (I), a combination including one, or two or more polymers. The combination is a polymer obtained by a reaction of the tetrahydric dianhydride and the diamine represent by the general formula (I). [in the formula (I), A1 and R1 are defined as the claim].

The present invention relates to an artificially synthesized gene optiHA containing codons for chicken partial tropism. Its reading frame contains 1707 bp nucleotides and encodes a total of 568 amino acids. The gene is compatible with H5 subtype highly pathogenic avian influenza virus A / Goose / GuangDong / 1 / 96(H5N1)[GD / 1 / 96(H5N1)]hemagglutinin (HA) gene has a nucleotide homology rate of 70%, an amino acid homology rate of 100%, and encodes the H5 subtype Hemagglutinin (HA) protein of avian influenza virus GD / 1 / 96 (H5N1). The invention also relates to the application of the gene as an immunogenic gene of H5 subtype influenza DNA vaccine and other genetic engineering vaccines.

The present invention related to methods and compositions comprising recombinant vectors comprising chimeric capsids and recombinant pseudotyped vectors with non-native capsid protein(s). The recombinant vectors of the invention confer an altered tropism that permits selective targeting of desired cells.

The present invention discloses a pearl nucleus processing method for culturing nucleated pearl which comprise the following steps: (1)Choosing pearl nucleus of 12-17mm diameter, (2) purifying pearl nucleus, washing pearl nucleus with 5-10% dilute hydrochloric acid, then washing repeatedly with limewater until the pearl nucleus is neutral in pH, placing pearl nucleus in boiled water for disinfection; (3) activating the immersed pearl nuleus, placing pearl nucleus in activation liquor and processed pearl nucleus is obtained. The present invention purifies the pearl nucleus and activate the pearl nucleus before transplanting that effectively promote the tropism of pearl oyster to pearl nuclus reduce the cut infection probability of nucleus-transplanting tissue quick the healing rate of wound, promote the forming quality of pearl sac, reduce the surface blemish forming rate of nucleated pearl, promote the yield of nucleated pearl.

The present invention belongs to the fiber producing method field, in particular relates to a method which uses a reclaimed PET bottle to produce the terylene preoriented filament in particular. The present invention mainly solves the problems of the prior art that the cost of raw materials for production is high, the drying efficiency is low, and the uniformity is poor, etc., and provides a method which uses the reclaimed PET bottle to produce the terylene preoriented filament with low cost of the raw materials for production, high drying efficiency, and good uniformity. The present invention has the major steps that the bottle piece of the PET bottle is produced; the bottle piece is continuously crystallized and dried by passing through a pre-crystallizer and a drying tower; the dried bottle piece directly enters a threaded rod extrusion machine, and is melted and extruded by a spinning threaded rod; the molten polyester material is continuously filtrated by a molten mass filter with a big filtering area of one level or two levels, to enter a spinning manifold; a thread line is cooled by passing through a cross air blasting, to wind to form the preoriented filament.

Methods to improve the tropism or other features of a virus are disclosed. Such methods can be used to prepare, e.g., DNA or plasmid libraries of variants of a gene encoding a viral capsid or envelope protein having a randomly inserted restriction site, libraries of viral clones with such variant genes with a randomly inserted restriction site or polypeptide sequence targeting a receptor expressed by a specific type of mammalian cells. Described are also methods to prepare mosaic viruses, i.e., viral particles wherein copies of one or more capsid or envelope proteins originate from different sources. These methods can be used to prepare mosaic viruses of a specific mixture of wild-type and mutant proteins, or of different types of mutant proteins.

Novel adeno-associated virus (AAV) isolates in nucleotide and amino acid forms and uses thereof are provided. The isolates show tropism for certain target tissues, such as blood stem cells, liver, heart and joint tissue, and may be used to transduce stem cells for introduction of genes of interest into the target tissues. Discrete modified portions of the cap gene, VP1, VP2, and VP3, may be used alone or in combination in the present methods.

Novel adeno-associated virus (AAV) vectors in nucleotide and amino acid forms and uses thereof are provided. The isolates show specific tropism for certain target tissues, such as blood stem cells, liver, heart and joint tissue, and may be used to transduce stem cells for introduction of genes of interest into the target tissues. Certain of the vectors are able to cross tightly controlled biological junctions, such as the blood-brain barrier, which open up additional novel uses and target organs for the vectors, providing for additional methods of gene therapy and drug delivery.

The present invention relates to a novel RNA picornavirus that is called Seneca Valley virus (“SVV”). The invention provides isolated SVV nucleic acids and proteins encoded by these nucleic acids. Further, the invention provides antibodies that are raised against the SVV proteins. Because SVV has the ability to selectively kill some types of tumors, the invention provides methods of using SVV and SVV polypeptides to treat cancer. Because SVV specifically targets certain tumors, the invention provides methods of using SVV nucleic acids and proteins to detect cancer. Additionally, due to the information provided by the tumor-specific mechanisms of SVV, the invention provides methods of making new oncolytic virus derivatives and of altering viruses to have tumor-specific tropisms.

Adenovirus serotypes differ in their natural tropism. The adenovirus serotypes 2, 4, 5 and 7 all have a natural affiliation towards lung epithelia and other respiratory tissues. In contrast, serotypes 40 and 41 have a natural affiliation towards the gastrointestinal tract. The serotypes described differ in at least capsid proteins (penton-base, hexon), proteins responsible for cell binding (fiber protein), and proteins involved in adenovirus replication. This difference in tropism and capsid protein among serotypes has led to the many research efforts aimed at redirecting the adenovirus tropism by modification of the capsid proteins.

The invention belongs to the new powder metallurgy process, and specifically to a special method for preparing an anisotropic SmCo based nanocrystalline rare earth permanent magnet. The invention breaks through the nano-crystal particle tropism problem of the SmCo based nanocrystalline rare earth permanent magnet nanocrystalline grain and obtains the nanocrystalline permanent magnet with obvious crystallographic anisotropism and magnetic anisotropism. The invention obtains the anisotropic nanocrystalline rare earth permanent magnet by the operations of adopting surfactant to auxiliary high-energy ball mill, orientating the pulse magnetic field, cold isostatic pressing and low-temperature pressurizing and sintering. The SmCo based nanocrystalline rare earth permanent magnet with obvious anisotropism can be obtained after sintering without any treatment.

The invention discloses a similar Chinese herbal medicine search method based on a probability topic model. The method includes the following steps: transforming Chinese herbal medicine information in 'China Great Pharmacopoeia' and 'Chinese Materia Medica' into digital text through an optical character recognition tool; extracting information such as efficacy, property and flavor and channel tropism of Chinese herbal medicines by using a regular expression, and building a Chinese herbal medicine information base; generating corresponding vector spaces according to efficacy, property and flavor and channel tropism attributes of the Chinese herbal medicines respectively, and regulating the vector spaces of the efficacy according to the probability topic model; and at last, calculating similarity of the efficacy, property and flavor and channel tropism attributes between the Chinese herbal medicines according to cosine coefficients, and generating a Chinese herbal medicine similarity database. A user inputs the name of a Chinese herbal medicine, and a system displays the Chinese herbal medicine and similar Chinese herbal medicines visually in the form of a relational graph through searching in the corresponding Chinese herbal medicine pair similarity information database. By means of the similar Chinese herbal medicine search method based on the probability topic model, relevant Chinese herbal medicines can be searched according to attribute similarity, and the method is of great significance for Chinese herbal medicine study and Chinese herbal medicine informatization.

The invention discloses an FP growth algorithm model-based traditional Chinese medicine formula data mining method and system. The system comprises the following steps of: pre-processing recorded traditional Chinese medicine original data; constructing an FP-tree data structure which takes characteristic items in a first transaction set as frequent items; arranging characteristic items corresponding transaction in the first transaction set according to the sequence of the frequent items in the FP-tree data structure, and generating a second transaction set, wherein the second transaction set comprises a plurality of data sets, each data set take included characteristic items as transactions and each transaction comprises the characteristic items, arranged before the characteristic items corresponding to the transactions, in the data set; carrying out an FP-growth algorithm on each data set and generating FP-tree sub-data structures in one-to-one correspondence with the plurality of data sets; and mining association rules between different effects and / or association rules between the mined effects and channel tropisms on the basis of a corresponding relationship between the characteristic items in the different FP-tree sub-data structures. According to the method and system disclosed by the invention, the information interferences in the retrieval process of the users can be decreased.

The invention discloses methods for screening and evaluating sperm tropism and a dedicated microfluidic control chip thereof. The microfluidic control chip is composed of an upper layer structure and a lower layer structure, and the surface of the upper layer structure and / or the lower layer structure includes at least one microfluid pipeline unit; each microfluid pipeline unit comprises an entrance pool, at least one main pipeline with one end communicated with the entrance pool, an exit pool communicated with the other end of the main pipeline, and at least one branch pipeline communicated with the main pipeline. While in use, a physical or chemical gradient field is formed in the main pipeline. A sample is added from the entrance pool, freely moves in the gradient field of the main pipeline, and selectively enters the branch pipelines. Through counting sperms in the branch pipelines, the sperm tropism can be quantitatively evaluated; a fluid which is insoluble or slightly soluble in a cell culture solution is introduced, an interface of the two fluids is formed at the entrance port of each branch pipeline, movement of cells between the main pipeline and the branch pipelines can be limited, so as to realize capture and recovery of the sperms having tropism.