10 results about "Heterotrophic bacteria" patented technology

The application discloses a method for constructing a space-layered composite particle by salt-tropism induction and application thereof, and comprises the following steps: inoculating functional bacteria into an anaerobic ammonia oxidation granular sludge system, so that the functional bacteria and the anaerobic ammonia oxidation granular sludge form a mixed system; the functional bacteria are heterotrophic bacteria with salt-tolerant characteristics Bacillus pumilus NJUST51 strain; the mixed system is operated under anaerobic conditions, and salt is gradually added into influent, so that the salinity is increased to 0.5% to 1.0%; under the action of salt selection pressure, the functional bacteria are enriched on the surface of the granules to form a space-layered structure, so that a composite granule system is constructed. The application realizes stable construction by salt-tropism induction, improves the salt resistance and toxicant resistance stability of the anaerobic ammonia oxidation system, and expands the application range of the system in treatment of high-salinity and organic-toxicity industrial wastewater.

The application relates to the sewage treatment technical field and discloses a wastewater co-metabolic treatment method based on MABR, gaseous primary substrates and air are introduced into the membrane cavity of the MABR reactor, and the gaseous primary substrates include C1-C4 hydrocarbons. The gaseous primary substrates and the air are diffused through the membrane cavity to form a microenvironment with high concentrations of gaseous primary substrates and dissolved oxygen on the outer surface of the membrane, so that the functional bacteria are enriched and grown; the gaseous primary substrates and the oxygen are diffused from the inside of the membrane cavity to the outside, which is beneficial to the utilization of the gaseous primary substrates by the functional bacteria, avoids the competition and consumption of the gaseous primary substrates by the heterotrophic bacteria in the liquid phase, ensures that the functional bacteria in the biofilm obtain stable nutrition supply, and greatly improves the co-metabolic efficiency.

The application discloses a short-range denitrification process nitrous oxide kinetics modeling and verification method, and specifically comprises the following steps: simplifying the ASM3 model, retaining the core biochemical processes and components related to the growth, storage and endogenous respiration of heterotrophic bacteria; expanding the reaction mechanism to construct the biochemical process involved in the short-range denitrification process to produce N2O; constructing a new way of directly using NO2- as an electron acceptor to produce N2O in the endogenous respiration stage of microorganisms; considering different electron donors and acceptors in each biochemical process, constructing a process rate equation; selecting high-quality short-range nitrification-denitrification experimental data published to test the constructed model; taking self-test data as a benchmark, iteratively fitting and calibrating key parameters, so that the model prediction value and the actual measured value are optimally fitted.

The application discloses a method for in-situ collection and statistics of marine cyanobacterial symbiotic system and free cyanobacteria, which comprises the following steps: firstly, multiple gradient filtration pretreatment is performed on seawater samples, and the samples are sequentially filtered through 5 μm, 3 μm (including secondary filtration and slight ultrasonic) and 0.22 μm filter membranes, so that the samples are preliminarily separated according to particle sizes and different samples are obtained, and false symbiotic systems are removed; then, flow cytometry sorting is performed, eukaryotic algae interference is removed according to the differences of five light signals of FSC, SSC, APC, PE and PerCP-cy5.5, and symbiotic cyanobacteria, symbiotic heterotrophic bacteria and free cyanobacteria are sorted out; and the sorting results can be verified through 16S amplicon sequencing. The application does not intervene in biochemistry in the whole process, can keep the original state and natural proportion of microorganisms to the maximum extent, and realizes in-situ, accurate separation and statistics of marine cyanobacterial symbiotic system and free cyanobacteria.

The application discloses a kind of based on activated sludge heterotrophic bacteria cultivation and sulfur autotrophic directional domestication bacterial agent and wastewater treatment method, the bacterial agent mainly with municipal wastewater treatment plant surplus activated sludge as raw material, first through heterotrophic bacteria cultivation to improve sludge activity and biomass, then under the synergistic effect of elemental sulfur, sodium bicarbonate and nitrate, sulfur autotrophic denitrification directional domestication is carried out, and efficient, stable, impact-resistant composite denitrification bacterial population is formed;The mature bacterial population after separation, purification, mixed expansion is made into bacterial agent, directly added to actual wastewater treatment project, to realize the depth denitrification of wastewater.The application does not depend on single strain, does not use expensive reagent, completely from ordinary activated sludge, using two-stage precise domestication process, retains the natural synergy of bacterial population, has the advantages of fast start, low cost, salt and low temperature tolerance, without additional carbon source, stable operation, etc., can significantly improve the denitrification efficiency and stability of wastewater treatment system.

This disclosure provides a method for removing organic matter from drinking water using bio-enhanced activated carbon, belonging to the field of water treatment technology. The method includes: passing the effluent from sand-filtered drinking water into a filter column filled with activated carbon to operate the filter column; wherein the organic matter in the effluent includes natural organic matter and persistent organic pollutants, and the concentration of manganese ions in the effluent is controlled at 0.1-0.2 mg / L; continuously operating the filter column for a preset time to form a biofilm on the surface of the activated carbon; wherein the biofilm contains heterotrophic bacteria, manganese redox bacteria, and electroactive microorganisms; the manganese redox bacteria convert manganese ions into biogenic manganese oxides; the continuous generation of biogenic manganese oxides promotes the formation of a stable C-O-Mn coordination structure on the activated carbon surface; the C-O-Mn coordination structure and the electroactive microorganisms jointly construct the electroactivated biochar interface, achieving enhanced removal of organic matter.

The application discloses a method for producing PHA by fermenting photosynthetic bacteria with CO2. The method comprises inoculating phototrophic bacteria into a photobioreactor containing an inorganic salt medium with CO2 as the sole carbon source, and performing photosynthetic autotrophic culture under illumination and CO2 aeration to proliferate the phototrophic bacteria to the stable growth phase. Then, the phototrophic bacteria are induced to secrete extracellular sugar through salt stress, and the culture is performed until the extracellular sugar concentration reaches a predetermined threshold. Then, heterotrophic bacteria are inoculated into the culture to form a co-culture system with the phototrophic bacteria, and the culture is continuously performed under illumination and CO2 aeration, so that the heterotrophic bacteria synthesize and accumulate PHA by using the extracellular sugar. Finally, the bacterial cells are collected, and intracellular PHA is extracted. The method separates and couples the growth and sugar production of the phototrophic bacteria and the PHA accumulation of the heterotrophic bacteria in time and function, realizes the biosynthesis of PHA with CO2 as the sole carbon source, solves the metabolic contradiction that a single microorganism cannot simultaneously and efficiently fix carbon and synthesize PHA, and can stably operate in a 5L and 20L photobioreactor.

This invention discloses an apparatus and method for treating domestic sewage and high-ammonia nitrogen wastewater using a segmented influent dual-short-cut anaerobic ammonia oxidation (AAO) denitrification technology. Wastewater is pumped in in three stages: the first stage provides sufficient carbon source to drive short-cut denitrification; the middle stage maintains ammonia nitrogen supply to ensure AAO; and the final stage limits carbon source to inhibit heterotrophic bacteria proliferation. In the anoxic stage, the carbon source from the domestic sewage drives short-cut denitrification to reduce nitrate to nitrite, coupled with AAO to achieve denitrification; the aerobic stage achieves short-cut nitrification and continuously supplies substrate for AAO, forming a nitrogen recycling system. A strategy of shortening settling time is adopted to screen and form a layered granular sludge structure with AAO bacteria as the core. The outer layer is enriched with short-cut nitrifying and denitrifying bacteria, while the inner anoxic zone retains AnAOB, thus improving biomass retention capacity. This invention achieves a total nitrogen removal rate of 96±0.8% under the condition of 650±60 mg / L of total nitrogen in the influent, with an anaerobic ammonia oxidation contribution rate of 83±2.6%, and eliminates the need for an external carbon source and produces no N2O emissions.

This utility model discloses a microbial biofilm attachment device that is easy to clean, comprising a cylindrical body, a fixed frame fixedly connected to the outer side of the cylindrical body, an inspection door on the outer side of the cylindrical body, and a rotating shaft rotatably connected to the bottom of the cylindrical body via bearings. Two slides are fixedly connected to the outer side of the rotating shaft, and each of the two slides has a groove at both ends. Two first slides and a second slide are slidably connected within the grooves. A biological carrier is provided on one side of each of the first and second slides, and guide rods are fixedly connected to both sides of the second slide. This utility model not only facilitates the disassembly and cleaning of the biological carrier, increasing cleaning efficiency, but also promotes the growth of heterotrophic bacteria by expanding the spacing and strengthens the dominant nitrifying bacteria population by compressing the spacing, increasing the applicability of the device. Furthermore, the rotating shaft is rotated by the water pump's suction force, further enhancing the device's convenience.