18 results about "Heterotrophic bacteria" patented technology

The application discloses a method for constructing a space-layered composite particle by salt-tropism induction and application thereof, and comprises the following steps: inoculating functional bacteria into an anaerobic ammonia oxidation granular sludge system, so that the functional bacteria and the anaerobic ammonia oxidation granular sludge form a mixed system; the functional bacteria are heterotrophic bacteria with salt-tolerant characteristics Bacillus pumilus NJUST51 strain; the mixed system is operated under anaerobic conditions, and salt is gradually added into influent, so that the salinity is increased to 0.5% to 1.0%; under the action of salt selection pressure, the functional bacteria are enriched on the surface of the granules to form a space-layered structure, so that a composite granule system is constructed. The application realizes stable construction by salt-tropism induction, improves the salt resistance and toxicant resistance stability of the anaerobic ammonia oxidation system, and expands the application range of the system in treatment of high-salinity and organic-toxicity industrial wastewater.

The application relates to the sewage treatment technical field and discloses a wastewater co-metabolic treatment method based on MABR, gaseous primary substrates and air are introduced into the membrane cavity of the MABR reactor, and the gaseous primary substrates include C1-C4 hydrocarbons. The gaseous primary substrates and the air are diffused through the membrane cavity to form a microenvironment with high concentrations of gaseous primary substrates and dissolved oxygen on the outer surface of the membrane, so that the functional bacteria are enriched and grown; the gaseous primary substrates and the oxygen are diffused from the inside of the membrane cavity to the outside, which is beneficial to the utilization of the gaseous primary substrates by the functional bacteria, avoids the competition and consumption of the gaseous primary substrates by the heterotrophic bacteria in the liquid phase, ensures that the functional bacteria in the biofilm obtain stable nutrition supply, and greatly improves the co-metabolic efficiency.

The application discloses a short-range denitrification process nitrous oxide kinetics modeling and verification method, and specifically comprises the following steps: simplifying the ASM3 model, retaining the core biochemical processes and components related to the growth, storage and endogenous respiration of heterotrophic bacteria; expanding the reaction mechanism to construct the biochemical process involved in the short-range denitrification process to produce N2O; constructing a new way of directly using NO2- as an electron acceptor to produce N2O in the endogenous respiration stage of microorganisms; considering different electron donors and acceptors in each biochemical process, constructing a process rate equation; selecting high-quality short-range nitrification-denitrification experimental data published to test the constructed model; taking self-test data as a benchmark, iteratively fitting and calibrating key parameters, so that the model prediction value and the actual measured value are optimally fitted.

The application discloses a method for in-situ collection and statistics of marine cyanobacterial symbiotic system and free cyanobacteria, which comprises the following steps: firstly, multiple gradient filtration pretreatment is performed on seawater samples, and the samples are sequentially filtered through 5 μm, 3 μm (including secondary filtration and slight ultrasonic) and 0.22 μm filter membranes, so that the samples are preliminarily separated according to particle sizes and different samples are obtained, and false symbiotic systems are removed; then, flow cytometry sorting is performed, eukaryotic algae interference is removed according to the differences of five light signals of FSC, SSC, APC, PE and PerCP-cy5.5, and symbiotic cyanobacteria, symbiotic heterotrophic bacteria and free cyanobacteria are sorted out; and the sorting results can be verified through 16S amplicon sequencing. The application does not intervene in biochemistry in the whole process, can keep the original state and natural proportion of microorganisms to the maximum extent, and realizes in-situ, accurate separation and statistics of marine cyanobacterial symbiotic system and free cyanobacteria.

The application discloses a kind of based on activated sludge heterotrophic bacteria cultivation and sulfur autotrophic directional domestication bacterial agent and wastewater treatment method, the bacterial agent mainly with municipal wastewater treatment plant surplus activated sludge as raw material, first through heterotrophic bacteria cultivation to improve sludge activity and biomass, then under the synergistic effect of elemental sulfur, sodium bicarbonate and nitrate, sulfur autotrophic denitrification directional domestication is carried out, and efficient, stable, impact-resistant composite denitrification bacterial population is formed;The mature bacterial population after separation, purification, mixed expansion is made into bacterial agent, directly added to actual wastewater treatment project, to realize the depth denitrification of wastewater.The application does not depend on single strain, does not use expensive reagent, completely from ordinary activated sludge, using two-stage precise domestication process, retains the natural synergy of bacterial population, has the advantages of fast start, low cost, salt and low temperature tolerance, without additional carbon source, stable operation, etc., can significantly improve the denitrification efficiency and stability of wastewater treatment system.

The utility model belongs to the technical field of water quality regulation and control, and particularly relates to a water quality regulation device for crab culture in saline-alkali soil. The device is used for a culture pond and comprises a floating assembly used for floating on the water surface; the air supply assembly is arranged on the floating assembly and is used for conveying air into water; the oxygen conveying assembly is used for being laid at the bottom of a water pool, the oxygen conveying assembly is connected to the air supply assembly, the air supply assembly can convey air into the oxygen conveying assembly, and the oxygen conveying assembly can release air at the bottom of the water pool; air or oxygen can overflow on the surface of the bottom mud at the bottom of the water tank, so that the oxygen content on the contact interface of the water body and the bottom mud is increased, the activity of heterotrophic bacteria is inhibited, and the accumulation of toxic substances is reduced, so that sulfur-containing organic matters in the bottom mud are decomposed under the condition of sufficient oxygen; the efficiency of mineralizing nitrogen-containing substances to release ammonia nitrogen is reduced, the rate of decomposition of organic sulfides is reduced, and accumulation of toxic substances is reduced.

This disclosure provides a method for removing organic matter from drinking water using bio-enhanced activated carbon, belonging to the field of water treatment technology. The method includes: passing the effluent from sand-filtered drinking water into a filter column filled with activated carbon to operate the filter column; wherein the organic matter in the effluent includes natural organic matter and persistent organic pollutants, and the concentration of manganese ions in the effluent is controlled at 0.1-0.2 mg / L; continuously operating the filter column for a preset time to form a biofilm on the surface of the activated carbon; wherein the biofilm contains heterotrophic bacteria, manganese redox bacteria, and electroactive microorganisms; the manganese redox bacteria convert manganese ions into biogenic manganese oxides; the continuous generation of biogenic manganese oxides promotes the formation of a stable C-O-Mn coordination structure on the activated carbon surface; the C-O-Mn coordination structure and the electroactive microorganisms jointly construct the electroactivated biochar interface, achieving enhanced removal of organic matter.

The invention discloses a construction and operation method of a hydrophobic membrane MABR biological membrane based on biphase regulation and control, and belongs to the technical field of sewage treatment. The invention develops a novel MABR biofilm culturing method, which can effectively reconcile the interface contradiction between a hydrophobic membrane material and hydrophilic microorganisms, not only can utilize heterotrophic bacteria to improve the microenvironment on the surface of the membrane, but also can prevent the heterotrophic bacteria from being excessively proliferated and blocking mass transfer through accurate process control; therefore, rapid, stable and function-oriented biological membrane construction on the surface of the durable hydrophobic membrane is realized.

The invention discloses a method for producing PHA (polyhydroxyalkanoate) by fermenting photosynthetic bacteria cultured by using a mixed carbon source. The method comprises the following steps: inoculating phototrophic bacteria into a photobioreactor filled with an inorganic salt culture medium taking CO2 as a unique carbon source, and introducing CO2 under illumination to carry out photoautotrophic culture, so that the phototrophic bacteria proliferate to a stable growth period; inducing the phototrophic bacteria to secrete extracellular sugar through salt stress until the concentration of the extracellular sugar reaches a preset threshold value; inoculating heterotrophic bacteria into the culture medium, co-culturing the heterotrophic bacteria and phototrophic bacteria, culturing by taking CO2 as a unique carbon source, supplementing extracellular sugar, and continuously culturing until PHA accumulation reaches a peak value; finally, thalli are collected, and intracellular PHA is extracted. According to the method, phototrophic bacterium growth and sugar production and heterotrophic bacterium PHA accumulation are separated and coupled in time and function, extracellular sugar supplement is started in the later stage of co-culture, CO2 and an organic carbon source are synergistically utilized, the method can stably operate in 5L and 20L photobioreactors, the advantage of carbon emission reduction in the early stage is kept, and the yield and the production intensity are improved through organic carbon supplement.

The invention relates to a divisional application with the application number of 2024110933028, and relates to the technical field of prawn aquaculture, in particular to application of an enzymolysis composite carbon source in prawn aquaculture. The application comprises a pond, and the pond is provided with a liquid oxygen supply assembly, an oxygenation pump assembly and a feed spreading device; the liquid oxygen supply assembly comprises a liquid oxygen input device arranged on one side of the pond and a liquid oxygen output device arranged on the liquid level of the pond, an organic carbon source is artificially added into the aquaculture water, the number of heterotrophic bacteria in the water is increased, nitrogen-containing compounds such as ammonia nitrogen in the water are converted into mycoprotein, and the liquid oxygen supply assembly is used for supplying liquid oxygen to the pond. According to the present invention, the biological flocculant capable of being directly ingested by the filter-feeding culture object is formed, such that the problem of detritus and feed retention in the culture water body can be solved, the bait reutilization can be achieved, the effects of water quality purification, water change amount reduction, feed saving, culture object survival rate improvement, yield increase and the like can be achieved, and the gastrointestinal tract load of the shrimp can be reduced through the automatic feeding; the nutrient absorption rate of the feed is improved, and the pollution to water quality is reduced.

The application discloses a method for producing PHA by fermenting photosynthetic bacteria with CO2. The method comprises inoculating phototrophic bacteria into a photobioreactor containing an inorganic salt medium with CO2 as the sole carbon source, and performing photosynthetic autotrophic culture under illumination and CO2 aeration to proliferate the phototrophic bacteria to the stable growth phase. Then, the phototrophic bacteria are induced to secrete extracellular sugar through salt stress, and the culture is performed until the extracellular sugar concentration reaches a predetermined threshold. Then, heterotrophic bacteria are inoculated into the culture to form a co-culture system with the phototrophic bacteria, and the culture is continuously performed under illumination and CO2 aeration, so that the heterotrophic bacteria synthesize and accumulate PHA by using the extracellular sugar. Finally, the bacterial cells are collected, and intracellular PHA is extracted. The method separates and couples the growth and sugar production of the phototrophic bacteria and the PHA accumulation of the heterotrophic bacteria in time and function, realizes the biosynthesis of PHA with CO2 as the sole carbon source, solves the metabolic contradiction that a single microorganism cannot simultaneously and efficiently fix carbon and synthesize PHA, and can stably operate in a 5L and 20L photobioreactor.

This invention discloses an apparatus and method for treating domestic sewage and high-ammonia nitrogen wastewater using a segmented influent dual-short-cut anaerobic ammonia oxidation (AAO) denitrification technology. Wastewater is pumped in in three stages: the first stage provides sufficient carbon source to drive short-cut denitrification; the middle stage maintains ammonia nitrogen supply to ensure AAO; and the final stage limits carbon source to inhibit heterotrophic bacteria proliferation. In the anoxic stage, the carbon source from the domestic sewage drives short-cut denitrification to reduce nitrate to nitrite, coupled with AAO to achieve denitrification; the aerobic stage achieves short-cut nitrification and continuously supplies substrate for AAO, forming a nitrogen recycling system. A strategy of shortening settling time is adopted to screen and form a layered granular sludge structure with AAO bacteria as the core. The outer layer is enriched with short-cut nitrifying and denitrifying bacteria, while the inner anoxic zone retains AnAOB, thus improving biomass retention capacity. This invention achieves a total nitrogen removal rate of 96±0.8% under the condition of 650±60 mg / L of total nitrogen in the influent, with an anaerobic ammonia oxidation contribution rate of 83±2.6%, and eliminates the need for an external carbon source and produces no N2O emissions.

The invention is applied to the technical field of biological sewage treatment, and discloses a biofilm formation starting method of a biofilm system based on a sulfur-based nano ceramic carrier, the method comprises the following steps: S1, nano ceramic carrier modification; s2, screening functional bacteria; s3, inoculating functional bacteria for biofilm formation; s4, biological membrane domestication; and S5, optimizing process parameters. According to the biofilm formation starting method of the sulfur-based nano ceramic carrier-based biofilm system, functional bacteria are screened from forest surface soil (0-20 cm, VSS / TSS 0.3-0.4) with rich vegetation, and high-activity heterotrophic bacteria, autotrophic nitrifying bacteria and sulfur autotrophic denitrifying bacteria are obtained through soil dilution, ultrasonic extraction, filtration and impurity removal and three rounds of beaker enrichment; a strategy of preferential spraying and biofilm formation of heterotrophic bacteria is adopted, and an extracellular polymeric substance secreted by the heterotrophic bacteria is used for providing a stable adhesion basis for subsequent autotrophic bacteria, so that the colonization speed of the autotrophic bacteria on the surface of the sulfur-based nano ceramic carrier is obviously accelerated.

This utility model discloses a microbial biofilm attachment device that is easy to clean, comprising a cylindrical body, a fixed frame fixedly connected to the outer side of the cylindrical body, an inspection door on the outer side of the cylindrical body, and a rotating shaft rotatably connected to the bottom of the cylindrical body via bearings. Two slides are fixedly connected to the outer side of the rotating shaft, and each of the two slides has a groove at both ends. Two first slides and a second slide are slidably connected within the grooves. A biological carrier is provided on one side of each of the first and second slides, and guide rods are fixedly connected to both sides of the second slide. This utility model not only facilitates the disassembly and cleaning of the biological carrier, increasing cleaning efficiency, but also promotes the growth of heterotrophic bacteria by expanding the spacing and strengthens the dominant nitrifying bacteria population by compressing the spacing, increasing the applicability of the device. Furthermore, the rotating shaft is rotated by the water pump's suction force, further enhancing the device's convenience.

The invention discloses a method for industrially producing polyhydroxyalkanoate by using photosynthetic bacteria. The method comprises the following steps: inoculating phototrophic bacteria into a photobioreactor filled with an inorganic salt culture medium taking CO2 as a unique carbon source, and introducing CO2 under an illumination condition to carry out photoautotrophic culture, so that the phototrophic bacteria proliferate to a stable growth period; then, inducing the phototrophic bacteria to secrete extracellular sugar through salt stress, and culturing until the extracellular sugar concentration reaches a preset threshold value; inoculating heterotrophic bacteria into the photobioreactor subjected to salt stress induction culture, forming a co-culture system with the phototrophic bacteria, and culturing in two stages, namely culturing by taking CO2 as a unique carbon source, supplementing extracellular sugar, and continuously culturing until PHA accumulation reaches a peak value; finally, thalli are collected, and intracellular PHA is extracted. According to the method disclosed by the invention, a photosynthetic bacteria group staged culture process is successfully amplified to a 200L industrial scale, and a feasible technical path is provided for industrial application of producing PHA through CO2 conversion of the photosynthetic bacteria group.

The invention belongs to the technical field of water treatment, and particularly relates to a method for enhancing removal of new pollutants through interaction of photoautotrophic algae and heterotrophic bacteria. The method comprises the following steps: carrying out mixed culture on photoautotrophic algae and heterotrophic bacteria according to a cell density ratio of 1: (10-10000), carrying out mixed culture until a carbon source in the system is completely consumed and the carbon source is in a starvation state to obtain the bacteria-algae interaction system, mixing the bacteria-algae interaction system with sewage or pollutants to be treated, and degrading the pollutants by virtue of the interaction of algae and bacteria. The carbon starvation stress condition induces heterotrophic bacteria and algae to form a compact aggregate structure, a high-strength extracellular electron transfer process is excited, a system is promoted to generate a large amount of active oxygen with strong oxidizing property in situ, and the pollutant removal effect can be remarkably improved.

The invention discloses a method for improving prawn quality and removing nutritive salt in culture water by using an algae-based culture system, which is characterized in that a prawn culture unit, an upper filter layer, a feeding regulation and control module, an illumination system and a bottom algae culture unit are arranged in a culture pond; ammonia nitrogen waste at the bottom of the culture pond is firstly nitrified and converted into nitrate through a biological membrane in the upper filtering layer; a carbon source is precisely added through the adding regulation and control module, heterotrophic bacteria in the water body are promoted to form biological flocs while the carbon nitrogen ratio is maintained, and inorganic nitrogen is further fixed and converted; mixed nutrient algae in the bottom algae culture unit synchronously absorb nitrogen and phosphorus nutritive salts and part of organic carbon under the illumination condition regulated and controlled by an illumination system, convert the nitrogen and phosphorus nutritive salts and part of organic carbon into frond biomass and release oxygen, so that the dissolved oxygen level of a water body is improved; the method has the advantages that the concentration of ammonia nitrogen, nitrite and phosphate is effectively controlled, and meanwhile, a stable and healthy water environment is created for prawn growth.

The invention discloses a short-cut nitrification coupling anaerobic ammonia oxidation reaction process and system for high organic nitrogen load, and relates to the technical field of organic wastewater treatment.The short-cut nitrification coupling anaerobic ammonia oxidation reaction process comprises the following steps that organic wastewater containing high-concentration organic nitrogen is fed into a hydrolysis ammoniation tank; breaking macromolecular nitrogenous organic matters in the organic wastewater into micromolecular organic matters by using hydrolytic bacteria in the hydrolytic ammonification tank, performing deamination on amino acid and nitrogenous monosaccharide by using ammonification bacteria to generate ammonia nitrogen, and degrading micromolecular organic pollutants by using facultative anaerobic heterotrophic bacteria; feeding the treated wastewater into an oxygen-limited oxidation pond, and oxidizing ammonia nitrogen in the wastewater into nitrite nitrogen by short-cut nitrifying bacteria in the oxygen-limited oxidation pond; feeding the treated wastewater into an anaerobic ammonium oxidation pond, and enabling ammonia nitrogen and nitrite nitrogen in the wastewater to generate nitrogen gas by anaerobic ammonium oxidation bacteria in the anaerobic ammonium oxidation pond; and carrying out mud-water separation on the treated wastewater. The method has the advantages of strong adaptability to high organic nitrogen load, high nitrogen removal efficiency, high organic nitrogen removal efficiency, fast reaction rate, no need of carbon source, low aeration energy consumption and low sludge yield.