47 results about "Heterotrophic bacteria" patented technology

The invention discloses a light-driven synthetic flora and application thereof. The light-driven synthetic flora comprises phototrophic bacteria and heterotrophic bacteria, the phototrophic bacteria are genetically modified synechococcus slender, and the gene modification comprises construction and expression of heterologous sucrose permease, glyceraldehyde dehydrogenase and sucrose synthase in the synechococcus slender; and the heterotrophic bacteria are the P.adami. According to the light-driven synthetic flora disclosed by the invention, carbon dioxide is converted into cane sugar through photosynthesis of phototrophic bacteria, and the cane sugar is secreted out of cells to provide a carbon source for heterotrophic bacteria; carrying out cell growth and polyhydroxyalkanoate synthesis on the heterotrophic bacteria by using cane sugar; therefore, the light-driven synthetic flora provided by the invention can realize direct conversion of carbon dioxide into polyhydroxyalkanoate without additional carbon sources, has the advantage of environmental friendliness, and provides a new economical and feasible scheme and approach for production of polyhydroxyalkanoate.

The invention belongs to the technical field of artificial wetland regenerated water treatment, and particularly relates to an enhanced removal method for nitrate nitrogen and organic matters in an artificial wetland regenerated water source. According to the method, glucose, manganese ions and copper ions are added into a regenerated water source, heterotrophic bacteria are enriched in a constructed wetland packed bed, metal ions are loaded in the constructed wetland packed bed, extracellular active enzymes are generated through stress reaction of the heterotrophic bacteria to the metal ions, and then the enzymolysis process of humus organic matters difficult to biodegrade is achieved. The biodegradability of the recycled water is improved, and finally, the removal process of nitrate nitrogen and organic matters is enhanced.

The application discloses a method for constructing a space-layered composite particle by salt-tropism induction and application thereof, and comprises the following steps: inoculating functional bacteria into an anaerobic ammonia oxidation granular sludge system, so that the functional bacteria and the anaerobic ammonia oxidation granular sludge form a mixed system; the functional bacteria are heterotrophic bacteria with salt-tolerant characteristics Bacillus pumilus NJUST51 strain; the mixed system is operated under anaerobic conditions, and salt is gradually added into influent, so that the salinity is increased to 0.5% to 1.0%; under the action of salt selection pressure, the functional bacteria are enriched on the surface of the granules to form a space-layered structure, so that a composite granule system is constructed. The application realizes stable construction by salt-tropism induction, improves the salt resistance and toxicant resistance stability of the anaerobic ammonia oxidation system, and expands the application range of the system in treatment of high-salinity and organic-toxicity industrial wastewater.

The application relates to a method for inactivating viruses in sewage. The method comprises the following steps: introducing heterotrophic bacteria into sewage to be inactivated; increasing the carbon-nitrogen ratio of the sewage during the activation and expansion of the heterotrophic bacteria; washing and resuspending the activated and expanded heterotrophic bacteria to form a heterotrophic bacteria liquid to be used; inoculating the heterotrophic bacteria liquid into the sewage and reducing the carbon-nitrogen ratio of the sewage, so that the heterotrophic bacteria produce an oxidative stress response to inactivate viruses. The method adjusts the carbon-nitrogen ratio to make the heterotrophic bacteria change from an eutrophic environment to an oligotrophic environment, activates the oxidative stress pathway of the heterotrophic bacteria, increases the generation of oxidative active species such as superoxide free radicals, causes oxidative damage to the virus shell or envelope, and thus promotes the inactivation of viruses. The method is simple and efficient, and has a wide application scenario.

The application relates to the sewage treatment technical field and discloses a wastewater co-metabolic treatment method based on MABR, gaseous primary substrates and air are introduced into the membrane cavity of the MABR reactor, and the gaseous primary substrates include C1-C4 hydrocarbons. The gaseous primary substrates and the air are diffused through the membrane cavity to form a microenvironment with high concentrations of gaseous primary substrates and dissolved oxygen on the outer surface of the membrane, so that the functional bacteria are enriched and grown; the gaseous primary substrates and the oxygen are diffused from the inside of the membrane cavity to the outside, which is beneficial to the utilization of the gaseous primary substrates by the functional bacteria, avoids the competition and consumption of the gaseous primary substrates by the heterotrophic bacteria in the liquid phase, ensures that the functional bacteria in the biofilm obtain stable nutrition supply, and greatly improves the co-metabolic efficiency.

The present application relates to the field of wastewater treatment, and discloses a deep denitrification method for landfill leachate in a waste transfer station, comprising the following steps: passing wastewater into an anaerobic tank to perform denitrification; passing the effluent from the anaerobic tank into an anoxic tank to perform short-cut denitrification and anaerobic ammonia oxidation; passing the effluent from the anoxic tank into an aerobic tank to perform aerobic and anaerobic ammonia oxidation by using aerobic and anaerobic ammonia-oxidizing bacteria respectively loaded on the outer layer and the inner layer of biological fillers; refluxing part of the sludge-water mixture at the end of the aerobic tank into the anoxic tank, and passing the rest into a membrane bioreactor to separate sludge and water, degrade organic matter by using heterotrophic bacteria under aeration, and simultaneously convert ammonia into nitrate; and refluxing part of the sludge-water mixture in the membrane bioreactor into the anaerobic tank. The deep denitrification method can realize good cooperation between multiple denitrification pathways, avoid the defects of a single denitrification pathway, has less dependence on carbon sources, has less aeration amount and sludge yield, and has lower requirements for denitrification conditions.

The present invention relates to a method for reducing carbon emissions in a sewage treatment process. It is applicable to environmental protection fields such as sewage treatment and pollution reduction and carbon reduction. The technical solution adopted by the present invention is: a method for reducing carbon emissions in a sewage treatment process, comprising: adding nano-ferroferric oxide in the sewage treatment process; introducing an anaerobic ammonia oxidation reaction in the sewage treatment process. Ferroferric oxide has electrical conductivity and can improve the electron transfer ability of microorganisms. In the biological denitrification process, the carbon source is quickly utilized by heterotrophic bacteria, and the electrons are transferred to N2O reducing functional bacteria through the interspecies electron transfer process, thereby promoting the conversion and utilization of organic matter and the reduction of N2O. At the same time, no carbon source consumption occurs during the denitrification process of the anaerobic ammonia oxidation reaction, which improves the carbon source utilization efficiency of heterotrophic bacteria and achieves the purpose of pollution reduction and carbon reduction. The present invention reduces the emissions of methane and nitrous oxide in the sewage treatment process by adding nano-ferroferric oxide in the sewage treatment process, thereby achieving a reduction in direct carbon emissions.

The application discloses a short-range denitrification process nitrous oxide kinetics modeling and verification method, and specifically comprises the following steps: simplifying the ASM3 model, retaining the core biochemical processes and components related to the growth, storage and endogenous respiration of heterotrophic bacteria; expanding the reaction mechanism to construct the biochemical process involved in the short-range denitrification process to produce N2O; constructing a new way of directly using NO2- as an electron acceptor to produce N2O in the endogenous respiration stage of microorganisms; considering different electron donors and acceptors in each biochemical process, constructing a process rate equation; selecting high-quality short-range nitrification-denitrification experimental data published to test the constructed model; taking self-test data as a benchmark, iteratively fitting and calibrating key parameters, so that the model prediction value and the actual measured value are optimally fitted.

The application discloses a method for in-situ collection and statistics of marine cyanobacterial symbiotic system and free cyanobacteria, which comprises the following steps: firstly, multiple gradient filtration pretreatment is performed on seawater samples, and the samples are sequentially filtered through 5 μm, 3 μm (including secondary filtration and slight ultrasonic) and 0.22 μm filter membranes, so that the samples are preliminarily separated according to particle sizes and different samples are obtained, and false symbiotic systems are removed; then, flow cytometry sorting is performed, eukaryotic algae interference is removed according to the differences of five light signals of FSC, SSC, APC, PE and PerCP-cy5.5, and symbiotic cyanobacteria, symbiotic heterotrophic bacteria and free cyanobacteria are sorted out; and the sorting results can be verified through 16S amplicon sequencing. The application does not intervene in biochemistry in the whole process, can keep the original state and natural proportion of microorganisms to the maximum extent, and realizes in-situ, accurate separation and statistics of marine cyanobacterial symbiotic system and free cyanobacteria.

The invention relates to the technical field of sewage treatment, in particular to a saccharide sodium acetate composite carbon source for enhanced denitrification in sewage and wastewater treatment. The composite carbon source is prepared by compounding starch-embedded saccharides, modified sodium acetate and trace elements consisting of iron, boron, selenium and molybdenum, the starch-embedded saccharides are prepared by gelatinizing and drying processes, and the modified sodium acetate is prepared by esterification reaction of sodium acetate and unsaturated alcohol. According to the composite carbon source, heterotrophic infectious microbe competition is inhibited through modified sodium acetate, slow-release supply of the carbon source is realized through starch embedding saccharides, and the denitrification key enzyme activity is activated by microelements, so that the problems of low denitrification efficiency, carbon source waste and high sludge yield of a traditional carbon source are solved through synergism of the three. The preparation process is simple, the raw materials are easy to obtain, the total nitrogen removal effect can be remarkably improved, the treatment cost is reduced, and the catalyst is suitable for denitrification nitrogen removal treatment of various water bodies such as municipal sewage and industrial wastewater and has a good industrial application prospect.

The invention relates to a method for inactivating viruses in sewage. The method comprises the following steps: introducing heterotrophic bacteria into sewage to be inactivated; the carbon-nitrogen ratio of the sewage is increased in the process of activating and expanding the heterotrophic bacteria; washing and resuspending the activated and propagated heterotrophic bacteria to form a standby heterotrophic bacteria solution; the heterotrophic bacteria liquid is inoculated into the sewage, and the carbon nitrogen ratio of the sewage is reduced, so that the heterotrophic bacteria generate oxidative stress reaction to inactivate viruses. According to the method, the carbon nitrogen ratio is regulated and controlled, so that the heterotrophic bacteria are converted from a eutrophication environment to a hypotrophic environment, an oxidative stress pathway of the heterotrophic bacteria is activated, generation of oxidative active species such as superoxide radicals is increased, oxidative damage to virus shells or envelopes is caused, and virus inactivation is promoted. The method is simple and efficient, and has a wide application scene.

The application discloses a kind of based on activated sludge heterotrophic bacteria cultivation and sulfur autotrophic directional domestication bacterial agent and wastewater treatment method, the bacterial agent mainly with municipal wastewater treatment plant surplus activated sludge as raw material, first through heterotrophic bacteria cultivation to improve sludge activity and biomass, then under the synergistic effect of elemental sulfur, sodium bicarbonate and nitrate, sulfur autotrophic denitrification directional domestication is carried out, and efficient, stable, impact-resistant composite denitrification bacterial population is formed;The mature bacterial population after separation, purification, mixed expansion is made into bacterial agent, directly added to actual wastewater treatment project, to realize the depth denitrification of wastewater.The application does not depend on single strain, does not use expensive reagent, completely from ordinary activated sludge, using two-stage precise domestication process, retains the natural synergy of bacterial population, has the advantages of fast start, low cost, salt and low temperature tolerance, without additional carbon source, stable operation, etc., can significantly improve the denitrification efficiency and stability of wastewater treatment system.

The application provides a wastewater and waste gas treatment device and method, which comprises a pool body and a gas-liquid mixed water distribution system; a flow guide area, an upflow area, a downflow area and an autotrophic bacteria treatment area are arranged in the pool body; the upflow area and the downflow area are arranged side by side between the flow guide area and the autotrophic bacteria treatment area, and each of the upflow area and the downflow area comprises a sulfate-reducing bacteria treatment section close to the flow guide area and a heterotrophic bacteria treatment section close to the autotrophic bacteria treatment area; an overflow area is arranged above the autotrophic bacteria treatment area; the gas-liquid mixed water distribution system comprises a first gas-liquid mixed passage connected with a water inlet pipe led out from the flow guide area and a water distribution pipe led out from the upflow / downflow area, and a second gas-liquid mixed passage connected with a circulating pipe led out from the overflow area and the water distribution pipe led out from the upflow / downflow area. The application can realize simultaneous nitrification and denitrification and strengthen the denitrification effect, and reduce the discharge amount of sulfate in sulfur autotrophic denitrification through autotrophic bacteria, heterotrophic bacteria and sulfate reduction, so that the efficiency is high and the energy consumption is small.

The utility model belongs to the technical field of water quality regulation and control, and particularly relates to a water quality regulation device for crab culture in saline-alkali soil. The device is used for a culture pond and comprises a floating assembly used for floating on the water surface; the air supply assembly is arranged on the floating assembly and is used for conveying air into water; the oxygen conveying assembly is used for being laid at the bottom of a water pool, the oxygen conveying assembly is connected to the air supply assembly, the air supply assembly can convey air into the oxygen conveying assembly, and the oxygen conveying assembly can release air at the bottom of the water pool; air or oxygen can overflow on the surface of the bottom mud at the bottom of the water tank, so that the oxygen content on the contact interface of the water body and the bottom mud is increased, the activity of heterotrophic bacteria is inhibited, and the accumulation of toxic substances is reduced, so that sulfur-containing organic matters in the bottom mud are decomposed under the condition of sufficient oxygen; the efficiency of mineralizing nitrogen-containing substances to release ammonia nitrogen is reduced, the rate of decomposition of organic sulfides is reduced, and accumulation of toxic substances is reduced.

The method for measuring the real yield of the sludge through O2 / CO2 tracking comprises the following steps: measuring O2 consumed by a microbial biochemical reaction in activated sludge and CO2 generated by the microbial biochemical reaction, and combining consumed NH3-N and COD (Chemical Oxygen Demand) concentration to synchronously measure the heterotrophic bacterium yield YH and the autotrophic bacterium yield YA; the synchronous measurement of the autotrophic bacteria and the heterotrophic bacteria can be realized at the same time, the actual value of the real yield of the sludge can be directly measured and calculated, the accuracy of the obtained real yield is not interfered by environmental factors, the measurement accuracy is high, the error is few, the cost is low, and the synchronous analysis of the competitive relationship between the autotrophic bacteria and the heterotrophic bacteria is facilitated.

This disclosure provides a method for removing organic matter from drinking water using bio-enhanced activated carbon, belonging to the field of water treatment technology. The method includes: passing the effluent from sand-filtered drinking water into a filter column filled with activated carbon to operate the filter column; wherein the organic matter in the effluent includes natural organic matter and persistent organic pollutants, and the concentration of manganese ions in the effluent is controlled at 0.1-0.2 mg / L; continuously operating the filter column for a preset time to form a biofilm on the surface of the activated carbon; wherein the biofilm contains heterotrophic bacteria, manganese redox bacteria, and electroactive microorganisms; the manganese redox bacteria convert manganese ions into biogenic manganese oxides; the continuous generation of biogenic manganese oxides promotes the formation of a stable C-O-Mn coordination structure on the activated carbon surface; the C-O-Mn coordination structure and the electroactive microorganisms jointly construct the electroactivated biochar interface, achieving enhanced removal of organic matter.

The utility model provides a composite cathode bioelectric desalting water treatment device. The composite cathode bioelectric desalting water treatment device comprises a membrane roll, an anode electrode, a cathode electrode, a bioelectric desalting device reactor, a water treatment filler layer and a desalting chamber, a cylindrical rolled membrane roll is arranged in the bioelectric desalting device reactor, a desalting chamber is arranged in a middle interlayer of the membrane roll, an anode chamber is arranged on one side of the membrane roll, a cathode chamber is arranged on the other side of the membrane roll, an anode electrode is arranged in the anode chamber, and a cathode electrode and a water treatment filler layer are arranged in the cathode chamber. And the anode electrode and the cathode electrode are connected to form a loop. A water treatment filler layer is added to a biological cathode of the bioelectric desalting equipment, aerobic cathode electrogenesis bacteria are enriched to a carbon-based electrode material in this way, nitrifying bacteria and part of heterotrophic bacteria are enriched into filler, spatial separation of the bacteria is achieved, and the treatment effect is improved.

The invention relates to a sludge biological conditioning rapid dehydration method and system based on complex flora synergy and dynamic regulation, and the method comprises the following steps: forming a complex flora by acidophilic autotrophic bacteria and acid-resistant heterotrophic bacteria, inoculating the complex flora into sludge, then adding a targeted nano nutritional agent into the sludge, and carrying out sludge biological conditioning under a dynamic aeration condition, the targeted nano nutritional agent is prepared from mesoporous SiO loaded nano FeS2 slow-release ferrous ions, anthraquinone-2-sodium sulfonate and N-P-K-Mg nutritive salt chelated by polyglutamic acid; the acid-resistant heterotrophic bacteria decompose dissolved organic matters (DOM) in the sludge to relieve the inhibition effect of acidophilic autotrophic bacteria, the dosage of the targeted nano nutritional agent is reduced to 3-5% of the dry weight of the sludge, and the biological conditioning time of the sludge is shortened to 8-12 hours by combining dynamic aeration regulation and control, so that the conditioning time is greatly shortened. The problems of acid corrosion and filter penetration are solved through the SiC-PTFE composite coating filter pressing belt, and the water content of the sludge is reduced to 55% or below.

To provide an innovative method for producing petals and a blooming promoter, which have not existed conventionally.SOLUTION: A method for producing petals is characterized in that flowers are cultivated with treated water W1, which is produced by subjecting aquaculture water W from an aquaculture part 1, where aquatic organism F is farmed, to treatment with microorganisms including nitrifying bacteria that convert ammonia in the aquaculture water W into nitrate and heterotrophic bacteria that decompose solid wastes such as feces and uneaten feed present in the aquaculture water W.SELECTED DRAWING: Figure 1

The invention discloses a construction and operation method of a hydrophobic membrane MABR biological membrane based on biphase regulation and control, and belongs to the technical field of sewage treatment. The invention develops a novel MABR biofilm culturing method, which can effectively reconcile the interface contradiction between a hydrophobic membrane material and hydrophilic microorganisms, not only can utilize heterotrophic bacteria to improve the microenvironment on the surface of the membrane, but also can prevent the heterotrophic bacteria from being excessively proliferated and blocking mass transfer through accurate process control; therefore, rapid, stable and function-oriented biological membrane construction on the surface of the durable hydrophobic membrane is realized.

The invention discloses a method for producing PHA (polyhydroxyalkanoate) by fermenting photosynthetic bacteria cultured by using a mixed carbon source. The method comprises the following steps: inoculating phototrophic bacteria into a photobioreactor filled with an inorganic salt culture medium taking CO2 as a unique carbon source, and introducing CO2 under illumination to carry out photoautotrophic culture, so that the phototrophic bacteria proliferate to a stable growth period; inducing the phototrophic bacteria to secrete extracellular sugar through salt stress until the concentration of the extracellular sugar reaches a preset threshold value; inoculating heterotrophic bacteria into the culture medium, co-culturing the heterotrophic bacteria and phototrophic bacteria, culturing by taking CO2 as a unique carbon source, supplementing extracellular sugar, and continuously culturing until PHA accumulation reaches a peak value; finally, thalli are collected, and intracellular PHA is extracted. According to the method, phototrophic bacterium growth and sugar production and heterotrophic bacterium PHA accumulation are separated and coupled in time and function, extracellular sugar supplement is started in the later stage of co-culture, CO2 and an organic carbon source are synergistically utilized, the method can stably operate in 5L and 20L photobioreactors, the advantage of carbon emission reduction in the early stage is kept, and the yield and the production intensity are improved through organic carbon supplement.

The present invention relates to a carrier for culturing anaerobic ammonia-oxidizing bacteria, as well as a preparation method and application thereof. The method comprises culturing heterotrophic bacteria that utilize an organic carbon source to the late logarithmic growth phase, harvesting bacterial cells, mixing the bacterial cells with chitosan, and then cross-linking with calcium carbonate to prepare a cross-linked chitosan carrier; and modifying the cross-linked chitosan carrier with polyethyleneimine to obtain a carrier for culturing anaerobic ammonia-oxidizing bacteria. The carrier provided by the present invention can provide a suitable growth environment for anaerobic ammonia-oxidizing bacteria, facilitate the adsorption and reproduction of anaerobic ammonia-oxidizing bacteria, prevent bacterial cell loss, and achieve a long-lasting and stable denitrification effect.

The invention relates to a full-chamber bed coupling biological denitrification reactor, which belongs to the technical field of sewage treatment equipment and comprises a reactor box body, a water distribution area, an upper fluidized bed reaction chamber, a lower fixed bed reaction chamber, a filtering area and a water outlet area are sequentially arranged in the reactor box body from top to bottom, and the water distribution area is connected with a water inlet pipe and a backwashing water outlet pipe. Heterotrophic denitrification biological carrier filler capable of suspending in water is arranged in the upper-layer fluidized bed reaction chamber, an autotrophic denitrification filler layer is arranged in the lower-layer fixed bed reaction chamber, a filter layer is arranged in the filter area, the water outlet area is connected with a water outlet pipe, a backwashing gas inlet pipe and a backwashing water inlet pipe, and stirring equipment is arranged in the upper-layer fluidized bed reaction chamber. According to the invention, heterotrophic bacteria and autotrophic bacteria are coupled for use, so that suspended colloid in water can be removed while total nitrogen is effectively removed, the addition amount of a carbon source is reduced, the occupied area and the tank capacity are small, and the investment of civil engineering and the like is greatly reduced; the denitrification system is stable in effluent, high in volume load, high in total nitrogen load and high in denitrification efficiency.

The invention provides a short-sludge-age high-ammonia-nitrogen wastewater variable-temperature treatment process and reaction system, and the process comprises the following steps: carrying out denitrification treatment in a high-temperature region denitrification tank under an anoxic condition, and converting nitrate nitrogen and nitrite nitrogen into nitrogen; the medium-temperature zone nitrification coupling denitrification tank converts and degrades ammonia nitrogen and residual organic matters in the denitrification mixed liquid through nitrifying bacteria nitrification reaction and heterotrophic bacteria oxidation reaction under the aerobic condition; part of nitrification mixed liquid flows back to the high-temperature area denitrification tank for nitrifying bacterium temperature domestication training; and carrying out dehydration treatment on the nitration mixed liquid to obtain dehydrated sludge and qualified clear liquid. According to the application, nitrate nitrogen and nitrite nitrogen in the wastewater are converted into nitrogen by utilizing denitrification reaction, residual ammonia nitrogen and organic matters are treated by utilizing nitration reaction, and a sludge mixture is subjected to dehydration treatment to obtain qualified clear liquid; the denitrification efficiency can be greatly improved in the whole treatment process; and the nitrifying bacteria can be subjected to temperature domestication, heat-resistant nitrifying bacteria are screened out, and the system operation is more stable.

The invention relates to a divisional application with the application number of 2024110933028, and relates to the technical field of prawn aquaculture, in particular to application of an enzymolysis composite carbon source in prawn aquaculture. The application comprises a pond, and the pond is provided with a liquid oxygen supply assembly, an oxygenation pump assembly and a feed spreading device; the liquid oxygen supply assembly comprises a liquid oxygen input device arranged on one side of the pond and a liquid oxygen output device arranged on the liquid level of the pond, an organic carbon source is artificially added into the aquaculture water, the number of heterotrophic bacteria in the water is increased, nitrogen-containing compounds such as ammonia nitrogen in the water are converted into mycoprotein, and the liquid oxygen supply assembly is used for supplying liquid oxygen to the pond. According to the present invention, the biological flocculant capable of being directly ingested by the filter-feeding culture object is formed, such that the problem of detritus and feed retention in the culture water body can be solved, the bait reutilization can be achieved, the effects of water quality purification, water change amount reduction, feed saving, culture object survival rate improvement, yield increase and the like can be achieved, and the gastrointestinal tract load of the shrimp can be reduced through the automatic feeding; the nutrient absorption rate of the feed is improved, and the pollution to water quality is reduced.

The application discloses a method for producing PHA by fermenting photosynthetic bacteria with CO2. The method comprises inoculating phototrophic bacteria into a photobioreactor containing an inorganic salt medium with CO2 as the sole carbon source, and performing photosynthetic autotrophic culture under illumination and CO2 aeration to proliferate the phototrophic bacteria to the stable growth phase. Then, the phototrophic bacteria are induced to secrete extracellular sugar through salt stress, and the culture is performed until the extracellular sugar concentration reaches a predetermined threshold. Then, heterotrophic bacteria are inoculated into the culture to form a co-culture system with the phototrophic bacteria, and the culture is continuously performed under illumination and CO2 aeration, so that the heterotrophic bacteria synthesize and accumulate PHA by using the extracellular sugar. Finally, the bacterial cells are collected, and intracellular PHA is extracted. The method separates and couples the growth and sugar production of the phototrophic bacteria and the PHA accumulation of the heterotrophic bacteria in time and function, realizes the biosynthesis of PHA with CO2 as the sole carbon source, solves the metabolic contradiction that a single microorganism cannot simultaneously and efficiently fix carbon and synthesize PHA, and can stably operate in a 5L and 20L photobioreactor.

This invention discloses an apparatus and method for treating domestic sewage and high-ammonia nitrogen wastewater using a segmented influent dual-short-cut anaerobic ammonia oxidation (AAO) denitrification technology. Wastewater is pumped in in three stages: the first stage provides sufficient carbon source to drive short-cut denitrification; the middle stage maintains ammonia nitrogen supply to ensure AAO; and the final stage limits carbon source to inhibit heterotrophic bacteria proliferation. In the anoxic stage, the carbon source from the domestic sewage drives short-cut denitrification to reduce nitrate to nitrite, coupled with AAO to achieve denitrification; the aerobic stage achieves short-cut nitrification and continuously supplies substrate for AAO, forming a nitrogen recycling system. A strategy of shortening settling time is adopted to screen and form a layered granular sludge structure with AAO bacteria as the core. The outer layer is enriched with short-cut nitrifying and denitrifying bacteria, while the inner anoxic zone retains AnAOB, thus improving biomass retention capacity. This invention achieves a total nitrogen removal rate of 96±0.8% under the condition of 650±60 mg / L of total nitrogen in the influent, with an anaerobic ammonia oxidation contribution rate of 83±2.6%, and eliminates the need for an external carbon source and produces no N2O emissions.

The invention discloses a conditioning agent for reducing ore leaching acid and heavy metal and a using method, the conditioning agent contains a microbial agent and lime milk, the microbial agent is sulfur reducing bacteria or acidophilic heterotrophic bacteria, the solid content of a microbial agent solution is 12%-25%, and the dosage of the microbial agent is 10%-30% of the mass of ore needing to be conditioned. The conditioner can adjust the pH value of the natural acid leaching environment of ore to form a certain stabilization effect, and the added biological agent competes with microorganisms related to acid production in the original environment to inhibit metabolism and growth of the microorganisms, reduce generation of acid and maintain the pH environment, so that the stable conditioning effects of weakening the acid environment and reducing heavy metal leaching are achieved.

The invention is applied to the technical field of biological sewage treatment, and discloses a biofilm formation starting method of a biofilm system based on a sulfur-based nano ceramic carrier, the method comprises the following steps: S1, nano ceramic carrier modification; s2, screening functional bacteria; s3, inoculating functional bacteria for biofilm formation; s4, biological membrane domestication; and S5, optimizing process parameters. According to the biofilm formation starting method of the sulfur-based nano ceramic carrier-based biofilm system, functional bacteria are screened from forest surface soil (0-20 cm, VSS / TSS 0.3-0.4) with rich vegetation, and high-activity heterotrophic bacteria, autotrophic nitrifying bacteria and sulfur autotrophic denitrifying bacteria are obtained through soil dilution, ultrasonic extraction, filtration and impurity removal and three rounds of beaker enrichment; a strategy of preferential spraying and biofilm formation of heterotrophic bacteria is adopted, and an extracellular polymeric substance secreted by the heterotrophic bacteria is used for providing a stable adhesion basis for subsequent autotrophic bacteria, so that the colonization speed of the autotrophic bacteria on the surface of the sulfur-based nano ceramic carrier is obviously accelerated.

This utility model discloses a microbial biofilm attachment device that is easy to clean, comprising a cylindrical body, a fixed frame fixedly connected to the outer side of the cylindrical body, an inspection door on the outer side of the cylindrical body, and a rotating shaft rotatably connected to the bottom of the cylindrical body via bearings. Two slides are fixedly connected to the outer side of the rotating shaft, and each of the two slides has a groove at both ends. Two first slides and a second slide are slidably connected within the grooves. A biological carrier is provided on one side of each of the first and second slides, and guide rods are fixedly connected to both sides of the second slide. This utility model not only facilitates the disassembly and cleaning of the biological carrier, increasing cleaning efficiency, but also promotes the growth of heterotrophic bacteria by expanding the spacing and strengthens the dominant nitrifying bacteria population by compressing the spacing, increasing the applicability of the device. Furthermore, the rotating shaft is rotated by the water pump's suction force, further enhancing the device's convenience.