64 results about "Archaea" patented technology

Provided herein are methods, compositions, and kits for determining the spatial location of target nucleic acids, including endogenous and exogenous target nucleic acids, in a biological sample using padlock probes and substrates with spatially barcoded capture probes. Also disclosed herein are methods for determining a presence and / or location of a microbe (e.g., archaea, fungi, bacteria) in a biological sample, e.g., by determining the presence and / or location of a microbial target nucleic acid in the biological sample.

Provided herein are engineered variants of archaeal polymerases that exhibit exonuclease-minus activity, enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, improved uracil-tolerance and / or reduce sequence-specific errors in polymerase-catalyzed nucleotide binding and extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases and forming binding complexes, and uses for conducting nucleic acid sequencing reactions.

The invention belongs to the field of food fermentation, and particularly discloses a fermentation process of pickled vegetables in old jars, which comprises the following steps: selecting fresh vegetables, carrying out irradiation sterilization pretreatment, carrying out primary micro-aerobic fermentation by using a compound bacteria solution containing specific microorganisms, and carrying out secondary anaerobic fermentation by using a steeping liquor with a specific ratio. In the process, the vegetables are diversified, the compound bacteria liquid is rich in various beneficial microorganisms such as halophilic archaea, lactic acid bacteria and yeast, the steeping liquor 1 and the steeping liquor 2 respectively optimize the fermentation environment and are rich in nutritional ingredients and flavor substances such as lactic acid bacteria exopolysaccharides, tea polyphenols and selenium-enriched yeast powder, and the taste and fragrance of the pickled vegetables are improved. Besides, according to the process, by accurately controlling the fermentation temperature, humidity and time, the fermentation period is remarkably shortened, meanwhile, the use of chemical additives is avoided, and the safety and health of the pickled vegetables are guaranteed; the prepared pickled vegetables in old jars are delicious in taste, rich in fragrance, short in fermentation time and free of chemical additives, and the requirements of consumers for high-quality and healthy foods are met.

The invention relates to a soil conditioner based on nitrification inhibition and a soil acid control method, and belongs to the technical field of soil conditioning, the soil conditioner comprises the following components by weight: 20-22 parts of humic acid, 32-36 parts of syringic acid-linoleic acid modified porous hydroxyapatite, 4-5 parts of complete nitrifying bacteria, 2-5 parts of syringic acid, and 2.8-4.2 parts of sodium carboxymethyl cellulose. According to the technical scheme, syringic acid and linoleic acid serve as nitrification inhibitors, the nitrification inhibition rate can be effectively increased, ecological niches of ammonia-oxidizing archaea and ammonia-oxidizing bacteria can be reduced through competitive occupation due to addition of complete nitrifying bacteria, the nitrification rate of the ammonia-oxidizing archaea and the ammonia-oxidizing bacteria is inhibited, and N2O emission is reduced. Humic acid and hydroxyapatite can be used as pH buffer substances to regulate and control soil acidity, aluminum toxicity can be relieved, and physical and chemical properties of soil can be improved; meanwhile, the complete nitrifying bacteria can be ensured to have relatively high activity under the condition that the pH is 5.5-6.5.

The invention provides application of pediococcus pentosaceus in preparation of a product for improving pork quality and intestinal flora, and relates to the technical field of animal feeding. The pediococcus pentosaceus disclosed by the invention is pediococcus pentosaceus LL2018368, is separated from the digestive tract of a Diqing Tibetan pig and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.18709. The pediococcus pentosaceus disclosed by the invention has the advantages that the pediococcus pentosaceus can be used for preparing the pediococcus pentosaceus; the pediococcus pentosaceus LL2018368 is added into drinking water of the Du-Si pigs, the influence of the pediococcus pentosaceus LL2018368 on archaea, meat quality, tissue fat-soluble vitamins and excrement metabolite composition in the excrement of the Du-Si pigs is studied, and the result shows that the pediococcus pentosaceus LL2018368 enables the species of the archaea in the excrement to tend to be consistent, so that the content of the archaea in the excrement of the Du-Si pigs in the excrement of the Du-Si pigs in the excrement of the Du-Si pigs is increased. The contents of vitamin A and all-trans-retinol in kidney tissues are increased, and the probiotic functions of amino acid and fat metabolism related bacteria and enzymes are improved.

The present invention relates to a functionally active mutated primase domain from an archaeal DNA primase belonging to the primase-polymerase family, comprising at least one amino acid substitution, wherein said mutated primase domain retains at least 50% of the template-independent terminal nucleotidyl transferase activity of the corresponding wild-type primase domain.

The present disclosure provides extruded or spun, semi-permeable, porous hollow fibres, comprising covalent ester, thioester and / or amide crosslinked polypeptides as well as processes for their production. The hollow fibres may be produced from protein, protein extracts, and / or protein isolates derived from plants, animals, bacteria, algae, archaea, and / or fungi, and in certain embodiments are intended to be suitable for human and / or animal ingestion. In some embodiments, the hollow fibres may be designed to be used in the production of cartridges that are compatible with existing and / or novel bioreactor platforms, for harbouring cell cultures in cultured meat production.

The present disclosure pertains to a Methanothermobacter wolfeii archaeal strain identified as BSEL1 and progeny and mutants thereof. The archaeal strain BSEL1 is capable of growing and producing renewable natural gas (RNG) from a supply of carbon dioxide (CO2) and hydrogen (H2) without the addition of organic supplements, and further to grow in wastewater with an exceptionally high growth rate. The strain is further capable of reducing CO2 to a very low level when grown, e.g., in a trickling bed reactor. BSEL1 is of major importance for direct addition of RNG into the natural gas grid.

The present disclosure provides extruded or spun, semi-permeable, porous hollow fibres, comprising covalent ester, thioester and / or amide crosslinked polypeptides as well as processes for their production. The hollow fibres may be produced from protein, protein extracts, and / or protein isolates derived from plants, animals, bacteria, algae, archaea, and / or fungi, and in certain embodiments are intended to be suitable for human and / or animal ingestion. In some embodiments, the hollow fibres may be designed to be used in the production of cartridges that are compatible with existing and / or novel bioreactor platforms, for harbouring cell cultures in cultured meat production.

The present disclosure provides extruded or spun semi-permeable porous hollow fibers comprising covalent ester, thioester, and / or amide cross-linked polypeptides and methods of producing the same. The hollow fibers may be produced from proteins, protein extracts and / or protein isolates derived from plants, animals, bacteria, algae, archaea and / or fungi and, in certain embodiments, are intended to be suitable for human and / or animal ingestion. In some embodiments, the hollow fibers may be designed for producing cartridges compatible with existing and / or novel bioreactor platforms for containing cell cultures in cultured meat production.

The invention provides a yellow liquid anaerobic digestion treatment method based on nitrate and manganese-loaded biochar, which comprises a treatment step of adding nitrate and manganese-loaded biochar into cellar bottom yellow liquid to be treated in an anaerobic digestion treatment link. According to the method, the manganese-loaded biochar is in contact with nitrate radicals, the nitrate radicals are converted into nitrogen free radicals with high activity, refractory organics in the yellow liquor are oxidized, and hydrolytic decomposition of the refractory organics in the yellow liquor is promoted. The biochar can also strengthen interspecific electron transfer between bacteria and archaea. Under the combined action of nitrate and manganese-loaded charcoal, not only is the activity level of anaerobic microorganisms driven to be improved, but also the chemical oxidation effect is introduced. Under the combined action of the two, the anaerobic digestion efficiency of the yellow liquid is comprehensively improved.

The present disclosure provides compositions and kits for the tagmentation of double stranded DNA. In some embodiments, the compositions and kits for the tagmentation of double stranded DNA include one or more histone-like proteins and / or one or more transposition systems. The present disclosure also provides methods for the tagmentation of double stranded DNA in the presence of one or more histone-like proteins.

Certain embodiments of the invention pertain to a method of treating an infection in a subject caused by an infectious agent other than Escherichia coli, the method comprising administering to the subject arsinothricin or a salt thereof. The infectious agent other than E. coli can be a bacterium, protozoan, helminth, archaebacterium, or a fungus. In preferred embodiments, the infectious agent is Mycobacterium tuberculosis, Mycobacterium bovis, or Enterobacter cloacae. The invention also pertains to a method of treating an infection in a subject caused by an infectious agent, comprising administering to the subject arsinothricin or a salt thereof in combination with an inhibitor of phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase. In certain such embodiments, the infectious agent expresses phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase. Further embodiments provide compositions comprising arsinothricin or a salt thereof and an inhibitor of phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase.

The present invention relates to adenine base editors (ABEs) and components thereof. The present invention also relates to complexes comprising an adenine base editor (ABE) and a guide RNA in a functionally associated form. The present invention further relates to a nucleic acid molecule encoding an ABE / guide RNA, an expression construct or vector comprising a nucleic acid sequence encoding an adenine base editor and / or a nucleic acid sequence encoding a guide RNA. The present invention further relates to a cell comprising an adenine base editor (ABE) and a method for adenine base editing of a target site in a genome of interest in at least one cell of a prokaryotic or eukaryotic organism, including bacterial and archaeal organisms. In addition, the present invention relates to various methods, kits and uses related to the provided ABEs.

The present invention relates to a method and a device for controlling the feed flow of at least one reactant in a methanation process. In particular, the present invention relates to a method and a device for iteratively controlling the feed flow of at least one reactant in a methanation process, which is carried out, for example, in a reactor such as an industrial-scale reactor and / or the reactor arrangement according to the invention. In particular, the present invention relates to a method and a device for iteratively controlling the feed flow of at least one reactant in a methanation process in an aqueous culture medium containing biocatalysts such as archaea.

The present invention provides a hard capsule comprising an outer and one inner capsule, wherein the outer capsule comprises a hydrophobic liquid and the inner capsule which comprises a composition comprising uncoated microorganisms and optionally at least one desiccant.The composition may comprise a filtrate composed of viable fecal bacteria useful for fecal microbiota transplantation (FMT) or a mixture of one or more different types of microorganisms isolated from intestinal microbiome, such as bacteria, archaea, virus, or fungi such as yeast.The composition may comprise a desiccant, preferably a food approved component which is degradable in the gastrointestinal tract. The hydrophobic liquid, if present, is preferably at least one edible oil.Also provided are methods of treating or preventing a dysbiosis in a human subject, comprising administering to the human subject at least one capsule of the invention.

The present invention refers to a method and a device for controlling the feed flow of at least a reactant of a methanation process. In particular, the present invention refers to a method and a device for iteratively controlling the feed flow of at least a reactant of a methanation process which e.g. is carried out in a reactor such as an industrial-size reactor and / or the reactor assembly according to the present invention. More particularly, the present invention refers to a method and a device for iteratively controlling the feed flow of at least a reactant of a methanation process in an aqueous culture medium comprising biocatalysts, such as Archaea.

The invention relates to the first hydrolase that specifically digests the archaeal type of a peptidoglycan (PG), the major component of the cell wall of methanogens, named Talosaminidase. The enzyme consists of a signal peptide, several bacterial IG domains, a glycosyl hydrolase, several archaeal PG binding domains and a peptidase domain. The double enzymatic activity for the glycosyl hydrolase and the peptidase maximizes the lysis of archaeal cells with a cell wall of the archaeal PG-type. Engineering the enzyme by removing the signal peptide and bacterial IG domains and keeping only the archaeal PG binding, glycosyl hydrolase and peptidase made the enzyme easier to express and purify, without negatively influencing the enzymatic activity. Therefore, engineered Talosaminidase can be used to regulate populations of archaeal methanogens in the industrial, agricultural and medical settings, and consequently methane production.

Disclosed herein are polynucleotides comprising sequences encoding coenzyme M synthase (ComF) linked to a heterologous regulatory element and methods of using the same. The polynucleotides may comprise synthetic operons comprising additional sequences encoding enzymes, e.g., a taurine-pyruvate aminotransferase, a sulfoacetaldehyde acetyl transferase, or a sulfopyruvate decarboxylase. Also disclosed herein are recombinant prokaryotic cells, e.g., recombinant bacterial, e.g., E. coli, or archaeal cells, e.g., Methanosarcina acetivorans with improved tolerance to oxidative stress.

The present invention relates to the use of a strain of probiotic bacteria or a derivative thereof to increase feed efficiency, enhance growth and / or productivity, improve body weight or body composition of a ruminant and / or increase milk production in a ruminant, inhibit the growth of methanogenic bacteria and / or archaea in the forestomach of a ruminant, reduce the ability of the ruminal microflora to produce methane, reduce methane emissions by the ruminant and / or reduce the greenhouse gas emission footprint of the ruminant. Ruminant feed compositions are also provided.

The present invention relates to probiotic strains or derivatives thereof for increasing feed efficiency, enhancing growth and / or productivity, improving body weight or body composition, and / or increasing milk yield in ruminants, inhibiting growth of methanogenic bacteria and / or archaea bacteria in the forestomach of ruminants, reducing the ability of rumen microbiota to produce methane, and / or increasing milk yield in ruminants. Use for reducing methane emissions from ruminants, and / or reducing the footprint of greenhouse gas emissions from ruminants. Ruminant feed compositions are also provided.

The invention relates to the technical field of heavy metal recovery equipment, and provides a heavy metal recovery device and method based on anaerobic methanophilic archaea, and the heavy metal recovery device based on anaerobic methanophilic archaea comprises an anode chamber, an anode assembly, a cathode chamber and a cathode assembly. The anode chamber is used for accommodating anaerobic methanophilic archaea, electrolyte and methane; the anode assembly is arranged in the anode chamber and is in contact with the anaerobic methanophilic archaea and the electrolyte; the cathode chamber is used for accommodating sewage containing heavy metals and is communicated with the anode chamber; a proton exchange membrane is arranged between the anode chamber and the cathode chamber; and the cathode assembly is arranged in the cathode chamber, is used for being in contact with sewage and is electrically connected with the anode assembly. According to the heavy metal recovery device based on the anaerobic methanophilic archaea, the anode chamber and the cathode chamber are separated through the proton exchange membrane, the anaerobic methanophilic archaea does not need to make direct contact with heavy metal ions, and the influence of the heavy metal ions on the activity of the anaerobic methanophilic archaea can be avoided.

The present invention refers to a method for the production of a whole-cut mycelial food substitute having fibrous structure optionally further comprising algae, bacteria, archaea or a combination thereof and a whole-cut mycelial food substitute obtainable by such method. In addition, the invention is directed to the use of a whole-cut mycelial food substitute for the production of a food product as well as such food product comprising an edible.

The application provides a gene and a protein sequence of a deep archaea transmethylase I and a preparation method thereof, belongs to the technical field of genetic engineering, and the transmethylase I is derived from a deep archaea genome and has transmethyl activity, so that the methyl in a methoxyl aromatic compound is transferred to a corrin ring protein. The deep archaea transmethylase I is cloned into a pCold-TF expression vector, is induced to express in Escherichia coli through a recombination expression vector, is purified through immobilized nickel ion affinity, and the transmethylase is prepared. The transmethylase I can be used for transmethylation of the methoxyl aromatic compound.

System and process are provided for fixation of carbon dioxide through fermentation. More specifically, the disclosure includes fermenting carbon dioxide into methane through methanogenic archaea and producing single cell protein nutrient supplement. The disclosure further provides the integration of methanogenic fermentation with additional processes to achieve improved carbon efficiency.

The invention discloses a construction method of an animal model with stable intestinal tract four-bound flora and an intestinal tract four-bound core flora used for evaluating the immune regulation function of infant blending powder. According to the method, milk powder base powder containing milk fat globule membranes is used for intervening SD male young rats in the second day of birth in a certain mode, and four flora models with stable intestinal bacteria, fungi, archaea and viruses of the young rats in the lactation period are constructed through metagenome sequencing. The infant blending powder base powder containing the milk fat globule membrane remarkably increases immune related species, and intestinal tract four-boundary core florae remarkably related to immunity are obtained through Spearman correlation analysis. The obtained intestinal tract four-bound flora model of the baby rats in the lactation period can be used for evaluating the immune regulation function of baby blending powder.

The present disclosure is related to compositions and methods comprising genetically modified Arthrospira platensis strains that express methanogen-killing lytic enzymes (methanolysins) to inhibit methane emission produced by enteric archaea.

The invention discloses a cross-queue multi-bound depression diagnosis marker screening method and system and a storage medium, and relates to the technical field of biochemical detection.The method comprises the steps that a multi-bound microorganism database is constructed, and the multi-bound microorganism database comprises bacteria, fungi, viruses and archaea; carrying out metagenome sequencing on feces of cross-queue patients and healthy controls based on the multi-bound microbial database to obtain a cross-queue species annotation abundance table; carrying out microorganism difference abundance analysis on the cross-queue species annotation abundance table by utilizing MMUPHin to obtain a difference species table; performing feature selection on the differential species table by using a Boruta algorithm in Python to obtain differential features; and constructing a prediction model based on the difference characteristics, and performing diagnosis marker screening by using the prediction model. According to the invention, by combining the depression diagnosis marker combination of cross-queue multi-boundary microorganisms, more comprehensive information can be provided for the diagnosis of depression, so that the accuracy and reliability of diagnosis are improved.

The present disclosure provides compositions and kits for fragmenting and tagging double stranded DNA. In some embodiments, the compositions and kits for fragmenting and tagging double stranded DNA comprise one or more histone-like proteins and / or one or more transposon systems. The present disclosure also provides methods for fragmenting and tagging double stranded DNA in the presence of one or more histone-like proteins.

The invention discloses application of a potato residual liquid bacterial fertilizer in apple planting, and relates to the technical field of agricultural planting. The invention relates to application of a potato residual liquid bacterial fertilizer in apple planting. The potato residual liquid bacterial fertilizer is prepared by the following steps: 1, separating archaea for more than one hundred years in an extreme environment; 2, purifying the archaea; 3, rejuvenating the archaea; 4, extracting the slave fungus from the roots of robust fruit trees such as apples and strawberries, then separating various slave fungus strains, determining through strain gene detection, and then classifying, purifying and rejuvenating; 5, archaea is cultivated on a PDA nutrient medium to form mother bacteria, then the mother bacteria are implanted into a proper culture medium for secondary propagation, and third propagation is conducted in the same step to obtain strains for standby application. Bacterial manure generated by fermenting potato residual liquid through the flora can effectively improve the quality of fruits such as apples and strawberries, balance the sweet-to-acid ratio and improve the taste, and the yield of the fruits is improved. The yield and the soil are improved. Meanwhile, the fertilizer utilization rate can be increased, the chemical fertilizer usage amount is reduced, and weight-losing and efficiency-increasing are