26 results about "Archaea" patented technology

Provided herein are methods, compositions, and kits for determining the spatial location of target nucleic acids, including endogenous and exogenous target nucleic acids, in a biological sample using padlock probes and substrates with spatially barcoded capture probes. Also disclosed herein are methods for determining a presence and / or location of a microbe (e.g., archaea, fungi, bacteria) in a biological sample, e.g., by determining the presence and / or location of a microbial target nucleic acid in the biological sample.

The invention relates to a soil conditioner based on nitrification inhibition and a soil acid control method, and belongs to the technical field of soil conditioning, the soil conditioner comprises the following components by weight: 20-22 parts of humic acid, 32-36 parts of syringic acid-linoleic acid modified porous hydroxyapatite, 4-5 parts of complete nitrifying bacteria, 2-5 parts of syringic acid, and 2.8-4.2 parts of sodium carboxymethyl cellulose. According to the technical scheme, syringic acid and linoleic acid serve as nitrification inhibitors, the nitrification inhibition rate can be effectively increased, ecological niches of ammonia-oxidizing archaea and ammonia-oxidizing bacteria can be reduced through competitive occupation due to addition of complete nitrifying bacteria, the nitrification rate of the ammonia-oxidizing archaea and the ammonia-oxidizing bacteria is inhibited, and N2O emission is reduced. Humic acid and hydroxyapatite can be used as pH buffer substances to regulate and control soil acidity, aluminum toxicity can be relieved, and physical and chemical properties of soil can be improved; meanwhile, the complete nitrifying bacteria can be ensured to have relatively high activity under the condition that the pH is 5.5-6.5.

The present disclosure provides extruded or spun semi-permeable porous hollow fibers comprising covalent ester, thioester, and / or amide cross-linked polypeptides and methods of producing the same. The hollow fibers may be produced from proteins, protein extracts and / or protein isolates derived from plants, animals, bacteria, algae, archaea and / or fungi and, in certain embodiments, are intended to be suitable for human and / or animal ingestion. In some embodiments, the hollow fibers may be designed for producing cartridges compatible with existing and / or novel bioreactor platforms for containing cell cultures in cultured meat production.

The invention provides a yellow liquid anaerobic digestion treatment method based on nitrate and manganese-loaded biochar, which comprises a treatment step of adding nitrate and manganese-loaded biochar into cellar bottom yellow liquid to be treated in an anaerobic digestion treatment link. According to the method, the manganese-loaded biochar is in contact with nitrate radicals, the nitrate radicals are converted into nitrogen free radicals with high activity, refractory organics in the yellow liquor are oxidized, and hydrolytic decomposition of the refractory organics in the yellow liquor is promoted. The biochar can also strengthen interspecific electron transfer between bacteria and archaea. Under the combined action of nitrate and manganese-loaded charcoal, not only is the activity level of anaerobic microorganisms driven to be improved, but also the chemical oxidation effect is introduced. Under the combined action of the two, the anaerobic digestion efficiency of the yellow liquid is comprehensively improved.

The present disclosure provides compositions and kits for the tagmentation of double stranded DNA. In some embodiments, the compositions and kits for the tagmentation of double stranded DNA include one or more histone-like proteins and / or one or more transposition systems. The present disclosure also provides methods for the tagmentation of double stranded DNA in the presence of one or more histone-like proteins.

The invention relates to the first hydrolase that specifically digests the archaeal type of a peptidoglycan (PG), the major component of the cell wall of methanogens, named Talosaminidase. The enzyme consists of a signal peptide, several bacterial IG domains, a glycosyl hydrolase, several archaeal PG binding domains and a peptidase domain. The double enzymatic activity for the glycosyl hydrolase and the peptidase maximizes the lysis of archaeal cells with a cell wall of the archaeal PG-type. Engineering the enzyme by removing the signal peptide and bacterial IG domains and keeping only the archaeal PG binding, glycosyl hydrolase and peptidase made the enzyme easier to express and purify, without negatively influencing the enzymatic activity. Therefore, engineered Talosaminidase can be used to regulate populations of archaeal methanogens in the industrial, agricultural and medical settings, and consequently methane production.

Disclosed herein are polynucleotides comprising sequences encoding coenzyme M synthase (ComF) linked to a heterologous regulatory element and methods of using the same. The polynucleotides may comprise synthetic operons comprising additional sequences encoding enzymes, e.g., a taurine-pyruvate aminotransferase, a sulfoacetaldehyde acetyl transferase, or a sulfopyruvate decarboxylase. Also disclosed herein are recombinant prokaryotic cells, e.g., recombinant bacterial, e.g., E. coli, or archaeal cells, e.g., Methanosarcina acetivorans with improved tolerance to oxidative stress.

The invention relates to the technical field of heavy metal recovery equipment, and provides a heavy metal recovery device and method based on anaerobic methanophilic archaea, and the heavy metal recovery device based on anaerobic methanophilic archaea comprises an anode chamber, an anode assembly, a cathode chamber and a cathode assembly. The anode chamber is used for accommodating anaerobic methanophilic archaea, electrolyte and methane; the anode assembly is arranged in the anode chamber and is in contact with the anaerobic methanophilic archaea and the electrolyte; the cathode chamber is used for accommodating sewage containing heavy metals and is communicated with the anode chamber; a proton exchange membrane is arranged between the anode chamber and the cathode chamber; and the cathode assembly is arranged in the cathode chamber, is used for being in contact with sewage and is electrically connected with the anode assembly. According to the heavy metal recovery device based on the anaerobic methanophilic archaea, the anode chamber and the cathode chamber are separated through the proton exchange membrane, the anaerobic methanophilic archaea does not need to make direct contact with heavy metal ions, and the influence of the heavy metal ions on the activity of the anaerobic methanophilic archaea can be avoided.

The application provides a gene and a protein sequence of a deep archaea transmethylase I and a preparation method thereof, belongs to the technical field of genetic engineering, and the transmethylase I is derived from a deep archaea genome and has transmethyl activity, so that the methyl in a methoxyl aromatic compound is transferred to a corrin ring protein. The deep archaea transmethylase I is cloned into a pCold-TF expression vector, is induced to express in Escherichia coli through a recombination expression vector, is purified through immobilized nickel ion affinity, and the transmethylase is prepared. The transmethylase I can be used for transmethylation of the methoxyl aromatic compound.

The invention discloses a device for synergistically degrading agriculture and forestry biomass by microorganisms for simulating a termite feeding behavior. The device comprises a pretreatment unit, a termite behavior simulation reaction cabin, a biological reaction system and a product separation and collection unit which are connected in sequence, the pretreatment unit comprises a crushing subunit and an ultrasonic humidifier; the termite behavior simulation reaction cabin comprises a mechanical bionic chewing unit for shearing and crushing agricultural and forestry biomass; the intelligent spraying system is used for spraying complex liquid containing a green surfactant; the microenvironment gradient regulation and control unit is used for simulating a termite intestinal tract gas environment; the time sequence fungicide adding device is used for adding fungi, bacteria and archaea in stages; the biological reaction system comprises a continuous stirred tank reactor; and the product separation and collection unit comprises a solid-liquid separation unit for separating solid residues and liquid products after biological reaction and a gas collection device for collecting and purifying biogas.

The present invention is concerned with device that is configured to be administered to the gut digestate of a ruminant animal, which device is capable of generating electrical energy from a gut digestate and / or measuring the concentration of hydrogen (H2) and / or oxygen (O2) that is present in the gut digestate. Further, the device according to the present invention may be modified to include an electrical load adjustment means (e.g. resistor, variable resistor etc) which may be used to adjust the electrical load of the device sufficient to cause the prescribed removal of H2, and in particular dissolved hydrogen (dH2), from the gut digestate. As such the device according to the present invention may be employed to adjust the amount of dH2 available to methanogenic archaea while at least not compromising animal productivity, thereby reducing the amount of methane released in the atmosphere which has an important environmental impact in terms of reducing greenhouse gas emissions.

The present invention provides compositions as additives for wastewater treatment. A first composition comprising archaea microorganism granules for bioaugmentation for treatment of COD in wastewater, and a second composition of activated silicate beads that allow removal of phosphate, nitrogen and suspended solids. The mixture of both compositions act synergistically by promoting organic matter degradation and allowing the removal of phosphate without consuming alkalinity of the treated solution.

This invention provides an amorphous iron-manganese oxide cluster, its preparation method, and its application in enhancing methanogenesis through functional enzymes. It belongs to the interdisciplinary technical fields of environmental functional materials, waste biological treatment, and resource utilization. The invention first mixes manganese salt and hydrochloric acid solution to obtain a hydrochloric acid solution of manganese salt; dissolves iron salt in water to obtain an iron salt solution; mixes the hydrochloric acid solution of manganese salt and the iron salt solution, heats the mixture, and then adds isopropanolamine solution to react, obtaining amorphous iron-manganese oxide clusters. The dispersion of the above amorphous iron-manganese oxide clusters is added to mixed bacterial granular sludge for sequential activation and anaerobic digestion. This can serve as an enhancer and protectant for anaerobic microbial functional enzymes, eliminating the influence of intermediate acids on the methanogenesis process through chelation or adsorption. It can also increase the adenine triphosphate yield of methanogenic archaea, accelerate archaea growth, and balance the growth differences between archaea and acidogenic bacteria.

Certain embodiments of the invention pertain to a method of treating an infection in a subject caused by an infectious agent other than Escherichia coli, the method comprising administering to the subject arsinothricin or a salt thereof. The infectious agent other than E. coli can be a bacterium, protozoan, helminth, archaebacterium, or a fungus. In preferred embodiments, the infectious agent is Mycobacterium tuberculosis, Mycobacterium bovis, or Enterobacter cloacae. The invention also pertains to a method of treating an infection in a subject caused by an infectious agent, comprising administering to the subject arsinothricin or a salt thereof in combination with an inhibitor of phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase. In certain such embodiments, the infectious agent expresses phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase. Further embodiments provide compositions comprising arsinothricin or a salt thereof and an inhibitor of phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase.

Certain embodiments of the invention pertain to a method of treating an infection in a subject caused by an infectious agent other than Escherichia coli, the method comprising administering to the subject arsinothricin or a salt thereof. The infectious agent other than E. coli can be a bacterium, protozoan, helminth, archaebacterium, or a fungus. In preferred embodiments, the infectious agent is Mycobacterium tuberculosis, Mycobacterium bovis, or Enterobacter cloacae. The invention also pertains to a method of treating an infection in a subject caused by an infectious agent, comprising administering to the subject arsinothricin or a salt thereof in combination with an inhibitor of phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase. In certain such embodiments, the infectious agent expresses phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase. Further embodiments provide compositions comprising arsinothricin or a salt thereof and an inhibitor of phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase.

Compositions and methods for improving biogas production during the treatment of organic waste, such as livestock, municipal, or industrial waste, are disclosed. The invention provides microbial compositions comprising specific genera of bacteria and archaea for enhancing methane production. The compositions are configured for introduction into a bioreactor to enhance the anaerobic digestion process, specifically increasing methane yield and / or accelerating waste degradation.

Examples of a mat are disclosed. The mat comprises a body with a first surface and a second surface, and a microbial component that comprises pre-determined microorganisms which are dispersed in the body of the mat between the first and second surfaces, on one of the first surface or the second surface, or on both of the first surface and the second surface. The microbial component manipulates a microbiome of an environment adjacent or in contact to the mat. The microbial component can comprise at least one of live bacteria, bacteria endospores, fungi, viruses, protozoa, algae, slime moulds, archaca, micro-plants, a synthetic biology system or microbial precursor that stimulate growth of the desired microorganisms.

The invention relates to the technical field of agricultural organic waste treatment, in particular to a method for treating agricultural organic waste containing high-concentration biological solid salt, and high-value fertilization of the waste is achieved through two-stage fermentation. In the first stage, a complex microbial inoculant composed of extremely halophilic archaea, high-salt-tolerant bacteria and the like is used for rapidly adsorbing and fixing free salt ions, the salt content of waste is reduced, and soil salinization is avoided. In the second stage, a complex microbial inoculant composed of lignin degrading bacteria, thermoactinomycetes and the like is adopted, complex organic matter is degraded under the oxygen-enriched condition and converted into stable substances such as humic acid, and meanwhile plant rhizosphere growth-promoting bacteria are planted, secrete growth-promoting substances and antagonize soil-borne diseases. The finally generated bio-organic fertilizer is rich in humic acid and active bacteria, can significantly improve the soil structure, enhance the water and fertilizer retention capability, improve the crop yield and quality, realize waste harmlessness and near-zero carbon emission, and avoid secondary pollution, and the method has both economic and environmental benefits.

The invention provides a pilot sequence, a payload delivery system constructed based on the pilot sequence and application of the payload delivery system. Wherein the preamble sequence is used to direct packaging of a payload into a protein complex, the preamble sequence comprising at least 20 amino acids at the N-terminus of an effector derived from archaea, Gram-negative or Gram-positive bacteria, the payload being a polypeptide, a nucleic acid, or a combination thereof. According to the application, more choices and resources are expanded for developing novel biological agents through research on the leader sequence.

The present application relates to a kind of soil conditioner based on nitrification inhibition and soil acid control method, belong to soil conditioning technical field, and soil conditioner includes the following weight fraction components: humic acid 20-22 parts, clove acid-linoleic acid modified porous hydroxyapatite 32-36 parts, complete nitrifying bacteria 4-5 parts, clove acid 2-5 parts, carboxymethyl cellulose sodium 2.8-4.2 parts.In the technical scheme of the present application, clove acid and linoleic acid as nitrification inhibitor, can effectively improve nitrification inhibition rate, and the addition of complete nitrifying bacteria can reduce ammonia-oxidizing archaea and ammonia-oxidizing bacteria niche by competitive occupation, inhibit the nitrification rate of ammonia-oxidizing archaea and ammonia-oxidizing bacteria, reduce N2O emission.Humic acid and hydroxyapatite can be used as pH buffer material, regulate soil acidity, also can alleviate aluminum toxicity, improve soil physical and chemical properties;Meanwhile, under pH 5.5-6.5, complete nitrifying bacteria can be guaranteed with higher activity.

The present disclosure pertains to a Methanothermobacter wolfeii archaeal strain identified as BSEL1 and progeny and mutants thereof. The archaeal strain BSEL1 is capable of growing and producing renewable natural gas (RNG) from a supply of carbon dioxide (CO2) and hydrogen (H2) without the addition of organic supplements, and further to grow in wastewater with an exceptionally high growth rate. The strain is further capable of reducing CO2 to a very low level when grown, e.g., in a trickling bed reactor. BSEL1 is of major importance for direct addition of RNG into the natural gas grid.

This invention discloses a *Serratia marcescens* strain for producing levonorgestrel and its applications, belonging to the field of bioengineering technology. This invention provides a method for producing levonorgestrel using the halophilic archaea MVA pathway in *Serratia marcescens*. The production and yield of levonorgestrel are increased through pathway construction, enhanced soluble expression of levonorgestrel synthase, and knockout of endogenous phospholipase. The engineered *Serratia marcescens* strain for producing levonorgestrel constructed in this invention achieves a yield of 73.4 g·L⁻¹ of levonorgestrel in a 30 L fermenter. ‑1 .

The present invention relates to a method for identifying microorganisms strain types, especially of bacterial or archaeal species and / or subspecies, using a single locus sequence typing technique in a sample. The present invention is furthermore related to a kit for use in said method, as well as to the use of a pharmaceutically effective amount of at least one antibacterial agent or at least one antiarchaeal agent against the strain types of bacterial or archaeal species and / or subspecies identified after step e) in the method according to the present invention for treating a human patient having a disease or health condition caused by or related to an infection with said bacterial or archaeal species and / or subspecies.

The present invention relates to an adenine base editor (ABE), and components thereof. The present invention also relates to a complex comprising an adenine base editor (ABE) and a guide RNA in a functionally associated form. The present invention further relates to a nucleic acid molecule encoding the ABE / guide RNA, an expression construct or a vector comprising a nucleic acid sequence encoding the adenine base editor and / or the nucleic acid sequence encoding the guide RNA. The present invention further relates to a cell comprising an adenine base editor (ABE) and a method of adenine base editing of a target site in a genome of interest in at least one cell of a prokaryotic organism, including bacterial and archaeal organisms, or eukaryotic organism. Besides that, the present invention relates to various methods, kits and uses associated with the ABEs provided.

The application relates to the technical field of biological detection, and provides a Cas3 / Cascade protein complex derived from a thermophilic archaea, which comprises TsiCas3 and TsiCascade; the TsiCas3 comprises Cas3HD and Cas3HEL, and the TsiCascade comprises Cas5, Cas6, Cas7, Cas8 and Cas11. The application further provides a CRISPR / Cas system, a nucleic acid molecule detection method based on the CRISPR / Cas system and application. The CRISPR / Cas system comprises the TsiCas3 / Cascade protein complex, an ssDNA probe and a guide RNA; once the target nucleic acid is combined with the guide RNA through hybridization, the TsiCas3 / Cascade protein complex is activated, and can cut non-target single-stranded DNA in disorder, and the detection method is used for detection.

Disclosed herein include methods, compositions, and kits for detecting a plurality of nucleic acid sequences. The method can comprise amplifying a first nucleic acid sequence and a second nucleic acid sequence in an amplification reaction mixture, thereby generating a first nucleic acid amplification product and a second nucleic acid amplification product. The method can comprise detecting in the same optic channel the first nucleic acid amplification product and the second nucleic acid amplification product with a first signal-generating oligonucleotide and a second signal-generating oligonucleotide, respectively.