10 results about "Archaea" patented technology

The present disclosure provides extruded or spun semi-permeable porous hollow fibers comprising covalent ester, thioester, and / or amide cross-linked polypeptides and methods of producing the same. The hollow fibers may be produced from proteins, protein extracts and / or protein isolates derived from plants, animals, bacteria, algae, archaea and / or fungi and, in certain embodiments, are intended to be suitable for human and / or animal ingestion. In some embodiments, the hollow fibers may be designed for producing cartridges compatible with existing and / or novel bioreactor platforms for containing cell cultures in cultured meat production.

The application provides a gene and a protein sequence of a deep archaea transmethylase I and a preparation method thereof, belongs to the technical field of genetic engineering, and the transmethylase I is derived from a deep archaea genome and has transmethyl activity, so that the methyl in a methoxyl aromatic compound is transferred to a corrin ring protein. The deep archaea transmethylase I is cloned into a pCold-TF expression vector, is induced to express in Escherichia coli through a recombination expression vector, is purified through immobilized nickel ion affinity, and the transmethylase is prepared. The transmethylase I can be used for transmethylation of the methoxyl aromatic compound.

The invention discloses a device for synergistically degrading agriculture and forestry biomass by microorganisms for simulating a termite feeding behavior. The device comprises a pretreatment unit, a termite behavior simulation reaction cabin, a biological reaction system and a product separation and collection unit which are connected in sequence, the pretreatment unit comprises a crushing subunit and an ultrasonic humidifier; the termite behavior simulation reaction cabin comprises a mechanical bionic chewing unit for shearing and crushing agricultural and forestry biomass; the intelligent spraying system is used for spraying complex liquid containing a green surfactant; the microenvironment gradient regulation and control unit is used for simulating a termite intestinal tract gas environment; the time sequence fungicide adding device is used for adding fungi, bacteria and archaea in stages; the biological reaction system comprises a continuous stirred tank reactor; and the product separation and collection unit comprises a solid-liquid separation unit for separating solid residues and liquid products after biological reaction and a gas collection device for collecting and purifying biogas.

The invention relates to the technical field of agricultural organic waste treatment, in particular to a method for treating agricultural organic waste containing high-concentration biological solid salt, and high-value fertilization of the waste is achieved through two-stage fermentation. In the first stage, a complex microbial inoculant composed of extremely halophilic archaea, high-salt-tolerant bacteria and the like is used for rapidly adsorbing and fixing free salt ions, the salt content of waste is reduced, and soil salinization is avoided. In the second stage, a complex microbial inoculant composed of lignin degrading bacteria, thermoactinomycetes and the like is adopted, complex organic matter is degraded under the oxygen-enriched condition and converted into stable substances such as humic acid, and meanwhile plant rhizosphere growth-promoting bacteria are planted, secrete growth-promoting substances and antagonize soil-borne diseases. The finally generated bio-organic fertilizer is rich in humic acid and active bacteria, can significantly improve the soil structure, enhance the water and fertilizer retention capability, improve the crop yield and quality, realize waste harmlessness and near-zero carbon emission, and avoid secondary pollution, and the method has both economic and environmental benefits.

The present application relates to a kind of soil conditioner based on nitrification inhibition and soil acid control method, belong to soil conditioning technical field, and soil conditioner includes the following weight fraction components: humic acid 20-22 parts, clove acid-linoleic acid modified porous hydroxyapatite 32-36 parts, complete nitrifying bacteria 4-5 parts, clove acid 2-5 parts, carboxymethyl cellulose sodium 2.8-4.2 parts.In the technical scheme of the present application, clove acid and linoleic acid as nitrification inhibitor, can effectively improve nitrification inhibition rate, and the addition of complete nitrifying bacteria can reduce ammonia-oxidizing archaea and ammonia-oxidizing bacteria niche by competitive occupation, inhibit the nitrification rate of ammonia-oxidizing archaea and ammonia-oxidizing bacteria, reduce N2O emission.Humic acid and hydroxyapatite can be used as pH buffer material, regulate soil acidity, also can alleviate aluminum toxicity, improve soil physical and chemical properties;Meanwhile, under pH 5.5-6.5, complete nitrifying bacteria can be guaranteed with higher activity.

The present disclosure pertains to a Methanothermobacter wolfeii archaeal strain identified as BSEL1 and progeny and mutants thereof. The archaeal strain BSEL1 is capable of growing and producing renewable natural gas (RNG) from a supply of carbon dioxide (CO2) and hydrogen (H2) without the addition of organic supplements, and further to grow in wastewater with an exceptionally high growth rate. The strain is further capable of reducing CO2 to a very low level when grown, e.g., in a trickling bed reactor. BSEL1 is of major importance for direct addition of RNG into the natural gas grid.

This invention discloses a *Serratia marcescens* strain for producing levonorgestrel and its applications, belonging to the field of bioengineering technology. This invention provides a method for producing levonorgestrel using the halophilic archaea MVA pathway in *Serratia marcescens*. The production and yield of levonorgestrel are increased through pathway construction, enhanced soluble expression of levonorgestrel synthase, and knockout of endogenous phospholipase. The engineered *Serratia marcescens* strain for producing levonorgestrel constructed in this invention achieves a yield of 73.4 g·L⁻¹ of levonorgestrel in a 30 L fermenter. ‑1 .

The present invention relates to a method for identifying microorganisms strain types, especially of bacterial or archaeal species and / or subspecies, using a single locus sequence typing technique in a sample. The present invention is furthermore related to a kit for use in said method, as well as to the use of a pharmaceutically effective amount of at least one antibacterial agent or at least one antiarchaeal agent against the strain types of bacterial or archaeal species and / or subspecies identified after step e) in the method according to the present invention for treating a human patient having a disease or health condition caused by or related to an infection with said bacterial or archaeal species and / or subspecies.

The application relates to the technical field of biological detection, and provides a Cas3 / Cascade protein complex derived from a thermophilic archaea, which comprises TsiCas3 and TsiCascade; the TsiCas3 comprises Cas3HD and Cas3HEL, and the TsiCascade comprises Cas5, Cas6, Cas7, Cas8 and Cas11. The application further provides a CRISPR / Cas system, a nucleic acid molecule detection method based on the CRISPR / Cas system and application. The CRISPR / Cas system comprises the TsiCas3 / Cascade protein complex, an ssDNA probe and a guide RNA; once the target nucleic acid is combined with the guide RNA through hybridization, the TsiCas3 / Cascade protein complex is activated, and can cut non-target single-stranded DNA in disorder, and the detection method is used for detection.

Disclosed herein include methods, compositions, and kits for detecting a plurality of nucleic acid sequences. The method can comprise amplifying a first nucleic acid sequence and a second nucleic acid sequence in an amplification reaction mixture, thereby generating a first nucleic acid amplification product and a second nucleic acid amplification product. The method can comprise detecting in the same optic channel the first nucleic acid amplification product and the second nucleic acid amplification product with a first signal-generating oligonucleotide and a second signal-generating oligonucleotide, respectively.