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Method for promoting anaerobic degradation of petroleum hydrocarbon in oil reservoir to produce methane

A technology for the production of methane and petroleum hydrocarbons, applied in the field of microbial gas production, can solve the problems of inappropriate activators for methanogens, and achieve the effect of promoting the degradation of petroleum hydrocarbons to produce methane

Pending Publication Date: 2018-02-13
BIOGAS SCI RES INST MIN OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

With the metabolic activities of microorganisms, the reservoir environment changes, and the initially screened methanogen activators are not suitable for the dynamically changing reservoir environment

Method used

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  • Method for promoting anaerobic degradation of petroleum hydrocarbon in oil reservoir to produce methane
  • Method for promoting anaerobic degradation of petroleum hydrocarbon in oil reservoir to produce methane
  • Method for promoting anaerobic degradation of petroleum hydrocarbon in oil reservoir to produce methane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Reservoir samples were collected from Shengli Oilfield, and the total DNA of the samples was extracted by the bead-beating method. The following methods were used to detect and analyze the microbial community structure in a certain reservoir to confirm that the reservoir contains methane-producing mutual hydrocarbon degradation bacteria:

[0035] (1) Functional gene detection of methanogenic bacteria that degrade mutual hydrocarbons in oil reservoir samples

[0036] Alkyl succinate synthase alpha subunit and benzyl succinate synthase alpha subunit genes assA and bssA detection references of hydrocarbon degrading bacteria CallaghanAV, Davidova IA, Savage-AshlockK, Parisi VA, Gieg LM, Suflita JM, Kukor JJ&Wawrik B(2010) Diversity of benzyl-and alkylsuccinatesynthase genes in hydrocarbon-impacted environments and enrichmentcultures.Environ Sci Technol 44(19):7287-7294. The PCR program is: 95℃ pre-denaturation 3min; 95℃ denaturation 45s, 55℃ Anneal for 1 min, extend at 72°C for ...

Embodiment 2

[0044] Taking the enrichment of the "simulated culture of petroleum hydrocarbon degradation and methanogenesis" in Example 1 as the test strain, cultivated at 55°C and 45°C respectively to construct petroleum hydrocarbon strain 1 and petroleum hydrocarbon strain 2; hydrogen-trophic methanogens Methanobacterium sp.CB 12 is an exogenous added methanogen, and the effect of the method of the present invention on the tendency of petroleum hydrocarbons to degrade and produce methane is analyzed.

[0045] (1) Prepare a sterile anaerobic medium for methanogens, cultivate hydrogen-trophic methanogens at 55°C anaerobicly to the logarithmic growth phase, collect the bacteria by centrifugation under anaerobic conditions, resuspend to a certain concentration, and place in anaerobic Store in the bottle at 4°C until use.

[0046] (2) Configure inorganic salt culture medium, inoculate petroleum hydrocarbon strain 1 or petroleum hydrocarbon strain 2 with 10% of the inoculum, and add hexadecane, 5×1...

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Abstract

The invention provides a method for promoting the anaerobic degradation of petroleum hydrocarbon in an oil reservoir to produce methane. The method comprises the following steps of (1), detecting andanalyzing the structure of a microbiologic population in the oil reservoir, so as to judge whether the oil reservoir contains a syntrophic hydrocarbon-degrading methanogenesis strain or not; (2), whenthe syntrophic hydrocarbon-degrading methanogenesis strain is contained in the oil reservoir, adding an additive A into the oil reservoir, and then shutting a well, wherein the additive is hydrogen nutritional methanogenesis archaea or mixed flora containing the hydrogen nutritional methanogenesis archaea; when the methanogenesis syntrophic hydrocarbon-degrading strain is not contained in the oilreservoir, adding an additive B into the oil reservoir, and then shutting the well, wherein the additive B is mixed bacteria containing the hydrogen nutritional methanogenesis archaea and syntrophichydrocarbon-degrading bacteria.

Description

Technical field [0001] The invention belongs to the technical field of microbial gas production, and specifically relates to a method for promoting methane production by anaerobic degradation of petroleum hydrocarbons in oil reservoirs. Background technique [0002] Reservoir is a typical extreme environment, complex environment, with the characteristics of high temperature, high pressure, high salinity, and lack of oxygen. At present, the average recovery rate of crude oil in my country is less than 35%. Many oil reservoirs for long-term water injection development and tertiary oil recovery (chemical flooding, microbial flooding, gas flooding, thermal oil recovery, etc.) exhibit high water cut, high recovery, and low permeability. It is one of the major problems of oil exploitation to improve the exploitation rate of low-grade oil reservoirs. [0003] After the deep underground oil reservoir is developed by water injection, a complex bio-biota system is formed. Although water inj...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P5/02E21B43/22
CPCC12P5/023E21B43/16Y02E50/30
Inventor 承磊黄艳黎霞代莉蓉刘来雁张辉
Owner BIOGAS SCI RES INST MIN OF AGRI
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