The invention discloses a treatment method of river polluted bed mud, comprising: adding oxidant into a polluted river to ensure that the bed mud on the surface layer is in the oxidation state; and preferably, adding water-solubility calcium salt and ferric salt. The invention ensures that the whole self-cleaning process of the river is in the aerobic state all the time by improving the oxidation reduction property of the polluted bed mud, thus creating conditions for aerobic microorganisms, amplifying bed mud aerobic microorganisms, and leading the aerobic microorganisms to digest organic pollutants; and in addition, the organic pollutants are completely oxidized and degraded into complete oxides such as CO2, H2O, NO2, SO4<-2> and the like, blackening and odor-causing substances, such as H2S, methyl mercaptan, FeS and the like produced during the anaerobic degradation of organic matters can be quickly oxidized, and the black and odor degree of water body can be effectively improved and remitted. Meanwhile, the calcium salt, the ferric salt and phosphorus-deposited nutritive salt are simultaneously added to lower river pollution degree and eutrophy level. The method of the invention has simple operation and strong selective pertinence, can treat in situ, almost does not damage the ecological balance of the original river and has favorable treatment effect.