The invention relates to a production process of bamboo charcoal fiber, comprising the following steps: pulverizing bamboo charcoal powder until the particle size is below the micron order, dispersing evenly; preparing cellulose triacetate from refined cotton linters as a raw material, dissolving the cellulose triacetate in dichloromethane to form a 10-50wt% spinning solution, adding bamboo charcoal micro powder to the spinning solution, wherein the weight ratio of the bamboo charcoal micro powder to the cellulose triacetate is 1:(50-100), and preparing bamboo charcoal acetate fiber by a dry spinning method. The invention has high performances of both bamboo charcoal fiber and acetate fiber, the manufactured fabric has the functions of moisture absorption, permeability, antibiosis, bacteriostasis, deodorization, high adsorption, temperature and humidity adjustment, far infrared radiation, electromagnetic radiation prevention and the like, and the fabric is soft, delicate, nonirritating and hypo-allergenic to skin, washable, sunfast, heat resistant, easy to wash, vermin-proof and moisture-proof.