53 results about "Hippophae" patented technology

The invention discloses a composite plant polypeptide composition with the effects of repairing pancreas and reducing blood sugar. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10 to 50 parts of ginsenoside, 5 to 30 parts of radix astragali seu hedysari extract, 3 to 20 parts of radix rehmanniae polysaccharide, 2 to 15 parts of radix trichosanthis alkaloid, 5 to 25 parts of rhizoma dioscoreae polysaccharide, 5 to 30 parts of bitter gourd polypeptide, 3 to 20 parts of folium mori alkaloid, 8 to 40 parts of rhizoma polygonati polysaccharide, 5 to 25 parts of fructus hippophae flavone, 10 to 35 parts of wheat germ peptide, 2 to 15 parts of coptis berberine, 10 to 45 parts of corn stigma polysaccharide and 3 to 20 parts of saussurea involucrata polyphenol. 1-10 parts of sophora flavescens total alkaloids, 5-25 parts of a dendrobium extract, 5-30 parts of a radix puerariae extract, 8-35 parts of L-arabinose and 10-40 parts of small molecule peptides; 20 to 100 parts of collagen peptide and 15 to 80 parts of albumin peptide; 5-50 parts of microcrystalline cellulose, 3-30 parts of fructo-oligosaccharide and 0.5-5 parts of magnesium stearate; the limitation of single sugar control or simple component mixing in the prior art is broken through, a synergistic system of plant active components and functional polypeptides is constructed, and the purity and retention rate of the plant active components are improved by optimizing the extraction process.

The invention discloses hippophae rhamnoides polysaccharide with the activity of reducing blood sugar and blood fat as well as a preparation method and application thereof. Adding water into the degreased hippophae rhamnoides, adjusting the pH value to be acidic, then heating, stirring and extracting under an ultrasonic condition, and collecting filtrate; and performing post-treatment to obtain the sea-buckthorn polysaccharide. The content of neutral sugar in the sea buckthorn polysaccharide is 25.31%-30.68%, the content of uronic acid in the sea buckthorn polysaccharide is 54.66%-59.79%, and the content of protein in the sea buckthorn polysaccharide is 1.91%-2.14%. According to the method, the sea-buckthorn polysaccharide is extracted by adopting acid assistance-ultrasonic extraction, and the prepared sea-buckthorn polysaccharide has remarkable blood sugar and blood fat reducing activity. When the concentration of the sea-buckthorn polysaccharide is as low as 0.1 mg / mL, the glucose uptake of HepG2-IR cells can still be obviously improved, and the content of total cholesterol and total triglyceride of the HepG2-IR cells can be obviously reduced. The hippophae rhamnoides polysaccharide can be applied to preparation of food or medicine for reducing blood sugar and / or blood fat.

The utility model relates to the related technical field of fruit juice filling, in particular to a sea-buckthorn fruit juice primary pulp filling machine which comprises a filling table, and a stirring mechanism is arranged at one end of the filling table. According to the hippophae rhamnoides fruit juice primary pulp filling machine, through the arrangement of the fixing frame, the liquid storage tank, the motor, a rotating rod, a rotating plate, a connecting column, a first gear, a first connecting rod, a second gear, a third gear and a second connecting rod, the motor is started during filling, and the motor enables the rotating rod to drive the rotating plate to rotate; at the moment, a second gear connected with the first gear in a meshed mode drives a first connecting rod and a third gear to rotate, a fourth gear connected with the third gear in a meshed mode drives a second connecting rod and a rotating block to rotate, and a rotating column at one end of the rotating block and a stirring frame rotate with the second connecting rod as the center; at the moment, the stirring frame also rotates by taking the rotating rod as the center, so that the hippophae rhamnoides fruit juice can be efficiently and comprehensively stirred, and the hippophae rhamnoides fruit juice is prevented from precipitating in the liquid storage tank to influence the taste.

The invention discloses an edible and medicinal compound hippophae rhamnoides flavone concentrated plant beverage and a preparation method thereof, belongs to the technical field of plant beverages, and aims to solve the problems that an existing hippophae rhamnoides product is single in function, high in sugar and low in flavone content, and centrifugal equipment is easy to block, poor in sealing and difficult to maintain. The invention provides a beverage formula which comprises the following components in parts by weight: 11-15 parts of hippophae rhamnoides raw pulp, 2-4 parts of erythritol, 11-13 parts of edible and medicinal compound water extract concentrated solution and 0.001-0.003 part of stevioside. The preparation method provided by the invention comprises the following steps: performing low-temperature quick-freezing, fine grinding, high-efficiency centrifugation and high-pressure homogenization preparation on sea-buckthorn primary pulp, performing staged micro-boiling extraction on an edible and medicinal compound water extraction concentrated solution, and then mixing in proportion; a special centrifugal device in the preparation method is provided with a double-anti-blocking, double-sealing and closed-loop rigid frame, and the beverage is high in flavone content, low in sugar, safe, synergistic in function, efficient, stable and easy to maintain, can solve industrial pain points and has remarkable technical innovation and application value.

The invention discloses a sea-buckthorn-derived T5H gene and application of an encoding protein thereof in improving the cold resistance of plants, and belongs to the technical field of biology. The hippophae rhamnoides-derived T5H gene and the encoded protein thereof can enhance the survival rate and cold resistance of plants (such as crops) under low-temperature stress, and have wide application prospects and popularization values in agricultural production practice.

The utility model discloses a hippophae rhamnoides oil microcapsule granulation device, which belongs to the technical field of microcapsule granulation and can realize integral screening treatment of particles in the subsequent granulation process, improve the integral practicability of the device and avoid secondary screening of the particles as much as possible. The hippophae rhamnoides oil microcapsule granulation device comprises a granulation cylinder, a material guiding pipe is installed at the bottom of the granulation cylinder, a screening cylinder is installed at the bottom of the material guiding pipe, a discharging pipe is installed at the bottom of the screening cylinder, the granulation cylinder, the material guiding pipe, the screening cylinder and the discharging pipe are internally communicated, a liquid guiding pipe is installed at the center of the top of the granulation cylinder, and an atomization spraying head is installed at the bottom of the liquid guiding pipe. An exhaust pipe is mounted at the top of the granulation cylinder, a collecting cover is mounted at the inner top of the granulation cylinder, the collecting cover is connected with the exhaust pipe, and a hot air assembly for assisting drying and granulation is mounted in the granulation cylinder; a filtering assembly assisting in screening is fixed in the screening cylinder, and a discharging assembly assisting in particle conveying out is installed in the discharging pipe.

The invention relates to a method for promoting growth, nodulation and nitrogen fixation of indigofera pseudotinctoria by utilizing rhizobium D303 and a bacterial solution thereof. The biological preservation number of the rhizobium sp. D303 is CCTCC (China Center for Type Culture Collection) NO: M20252803. Pot culture tie-back tests prove that the indigofera pseudotinctoria seedlings inoculated with the rhizobium are obviously superior to a control group in the aspects of plant height, root length, overground dry weight, underground dry weight, root nodule number, root nodule weight, root nodule diameter, nitrogenase activity, antioxidant enzyme activity and the like. The rhizobium strain provided by the invention has efficient nodulation and nitrogen fixation capability and excellent growth promoting characteristic, obviously improves the growth speed, nodulation capability and nitrogen fixation efficiency of the indigofera pseudotinctoria, reduces the dependence on chemical nitrogen fertilizer, and has a broad application prospect in development of special biological bacterial fertilizer for indigofera pseudotinctoria. The method has important ecological and economic benefits for promoting the application of the indigofera pseudotinctoria in ecological restoration and feed production, and is suitable for slope greening, water and soil conservation and barren mountain ecological restoration projects.

The invention relates to a method for establishing an agrobacterium tumefaciens-mediated halostachys sieboldii transient transformation system, and belongs to the technical field of plant genetic engineering. The method comprises the following steps: culture of aseptic seedlings, acquisition of explants, preparation of an infection solution containing agrobacterium tumefaciens, agrobacterium tumefaciens infection, co-culture, GFP verification and GUS staining. The method disclosed by the invention is simple and convenient to operate, short in period and capable of realizing high-efficiency expression of the target gene in the halostachys sieboldii tissue. According to the system, an instantaneous transformation platform is established in the halostachys sieboldii for the first time, and a key technical support is provided for functional analysis and stress resistance molecular mechanism research of the halostachys sieboldii.

The present application relates to the technical field of Hippophae rhamnoides planting, and discloses a Hippophae rhamnoides planting method for preventing and treating diseases and pests. According to the characteristics that Cossus hippophaeae searches by olfaction, the Hippophae rhamnoides and lilac trees are planted at intervals, the volatile odor of lilac is fully utilized to interfere with Cossus hippophaeae, so that Cossus hippophaeae cannot accurately find the Hippophae rhamnoides, and further cannot bore the Hippophae rhamnoides, thereby improving the survival rate of the Hippophae rhamnoides. In May to July every year, the sex pheromone is placed in the planting area, so that the male and female Cossus hippophaeae adults in the mating period cannot distinguish each other, and further cannot complete the copulation and oviposition, thereby greatly reducing the reproduction amount of Cossus hippophaeae. The present application can effectively prevent and treat Cossus hippophaeae by the mixed planting mode of Hippophae rhamnoides and lilac trees and the sex pheromone, the comprehensive cost is reduced, the survival rate of the Hippophae rhamnoides is greatly improved, and further the yield and quality of the Hippophae rhamnoides fruits are improved, so that the present application has high practical value and popularization value.

The invention relates to a preparation method of a hippophae rhamnoides leaf isorhamnetin glycoside enriched solid beverage, and belongs to the field of functional plant beverage processing. The preparation method comprises the following steps: preparing sea buckthorn green tea and sea buckthorn green tea freeze-dried powder by taking sea buckthorn leaves as a main raw material; carrying out ethanol extraction and AB-8 type macroporous resin separation and purification to obtain an isorhamnetin glycoside substance enriched component; a Maillard composite system formed by whey protein isolate and maltodextrin is loaded with an isorhamnetin glycoside substance enrichment component, the isorhamnetin glycoside substance enrichment component is compounded with sea buckthorn green tea freeze-dried powder, freeze drying is performed, and the isorhamnetin glycoside enrichment solid beverage is obtained. The isorhamnetin glycoside enriched solid beverage disclosed by the invention has good solubility, and shows a remarkable immunological enhancement effect in a cyclophosphamide induced immunocompromised mouse model. The method has a wide application prospect in the aspects of high-value utilization of hippophae rhamnoides leaf resources and functional beverage development.

The utility model discloses a hippophae rhamnoides fruit picking and collecting device, and relates to the technical field of hippophae rhamnoides fruit picking, the hippophae rhamnoides fruit picking and collecting device comprises a picking basket, a filter area is arranged in the picking basket, the bottom of the filter area is provided with a filter screen used for blocking branches and leaves, a collecting area is arranged below the filter area, and the bottom of the collecting area is provided with a blanking port; a vibration device is arranged on the filter screen; the picking basket is provided with a hanging face, and straps and shoulder straps are arranged on the hanging face. The hippophae rhamnoides fruit screening device is simple in structure, low in cost and capable of conducting preliminary screening on hippophae rhamnoides fruits in the field picking stage and removing large branches, leaves and other sundries, even if small sundries are left, the small sundries can be removed in the follow-up washing work, and the beneficial effect of reducing the workload of the follow-up work is achieved.

This invention relates to the field of plant growth regulation technology, specifically disclosing a cultivation method for promoting the germination of wild sea buckthorn seeds. The main inventive concept lies in the synergistic application of amino oligosaccharides (AS) and sodium nitroprusside (SNP) in promoting the germination and hypocotyl elongation of wild sea buckthorn seeds. Using AS alone (optimal concentration 0.12 mmol / L) or SNP alone (optimal concentration 0.09 mmol / L) can significantly improve the germination rate (GP) and germination index (GI) of sea buckthorn seeds, while simultaneously promoting hypocotyl elongation. Combining AS (0.12 mmol / L) and SNP (0.08 mmol / L) in a 1:1 ratio produces a synergistic effect, promoting the germination of wild sea buckthorn seeds, increasing seed α-amylase activity, and promoting hypocotyl elongation. This invention provides a low-cost, high-efficiency technical solution for efficient sea buckthorn seedling cultivation, applicable to ecological restoration and economic forest cultivation.

This invention belongs to the field of microbial technology, specifically relating to a biocontrol bacterium Z9 for controlling sea buckthorn wilt and its application. The *Bacillus polymyxa* described in this invention can control sea buckthorn wilt, achieving an inhibition rate of over 60% against its pathogen, *Fusarium cladosporium*, and demonstrating a disease control effect exceeding 60%. Pot and field experiments have confirmed that *Bacillus polymyxa* Z9 can effectively inhibit the growth of the sea buckthorn wilt pathogen and enhance the biocontrol efficacy against the disease.

The application discloses a traditional Chinese medicine compound composition, preparation and a preparation method thereof for treating acute radiation cystitis, and belongs to the technical field of medicines. The traditional Chinese medicine compound composition for treating acute radiation cystitis is prepared based on the screening of compound components by means of modern molecular biology and the theory of traditional Chinese medicine compound, and is prepared from Lysimachia christinae 2-4 parts, Herba Epimedii 1-3 parts, Herba Patriniae 2-4 parts, Smilax glabra 2-4 parts, Sophora flavescens 0.8-2.8 parts, Lobelia 1-3 parts, Semen Lagenariae 1.4-3.4 parts, Hippophae rhamnoides 1-3 parts, Herba Erigerontis 1-3 parts and Glycyrrhiza 1-3 parts by extraction. The traditional Chinese medicine compound can solve the problems of lack of targeted mechanism intervention, limited curative effect, large side effect and poor patient compliance in the prior art; in addition, the application proposes key target points and effective components of drugs for treating acute radiation cystitis, and experiments prove that the treatment effect on acute radiation cystitis is obvious.

The invention discloses a traditional Chinese medicine compound composition for treating acute radiation cystitis, a preparation and a preparation method thereof, and belongs to the technical field of medicines. The traditional Chinese medicine compound composition for treating acute radiation cystitis is prepared by screening compound components on the basis of modern molecular biology means and combining the traditional Chinese medicine compound theory. The traditional Chinese medicine compound composition for treating acute radiation cystitis is prepared by extracting 2-4 parts of lysimachia christinae hance, 1-3 parts of herba epimedii, 2-4 parts of herba patriniae, 2-4 parts of rhizoma smilacis glabrae, 0.8-2.8 parts of radix sophorae flavescentis, 1-3 parts of Chinese lobelia, 1.4-3.4 parts of semen cuscutae, 1-3 parts of sea-buckthorn, 1-3 parts of erigeron breviscapus and 1-3 parts of liquorice. The traditional Chinese medicine compound can solve the problems of lack of targeted mechanism intervention, limited curative effect, large side effect, poor patient compliance and the like in the existing treatment; in addition, the invention provides key targets and effective components of drugs for acute radiation cystitis, and experiments prove that the pharmaceutical composition has an obvious treatment effect on acute radiation cystitis.

The invention discloses a method for enriching narcissoside from hippophae rhamnoides, an obtained hippophae rhamnoides extract and application of the hippophae rhamnoides extract, and the method comprises the following steps: (1) crushing dried hippophae rhamnoides fruits, sieving with a 40-80-mesh sieve to obtain hippophae rhamnoides raw material powder, performing alcohol extraction on the obtained hippophae rhamnoides raw material powder to obtain a crude hippophae rhamnoides concentrating the obtained hippophae rhamnoides crude extract under reduced pressure, and freeze-drying to obtain hippophae rhamnoides crude extract (2) dissolving the obtained hippophae rhamnoides crude extract powder in 5-15% ethanol, and passing through a macroporous resin column; (3) cleaning the resin with deionized water and 15-25% ethanol in sequence to remove high-polarity impurities such as saccharides, organic acids and phenolic acids; and eluting with 35-45% ethanol, collecting the eluent to obtain a narcissoside enriched solution, concentrating under reduced pressure, and freeze-drying to obtain the sea-buckthorn extract, the narcissoside in the sea-buckthorn extract accounts for 50-70% of the total phenol by mass. The sea-buckthorn extract enriched with narcissoside has the skin effects of resisting wrinkles and saccharification, and has a relatively high application value.

The invention discloses a method for producing eurotium cristatum and hippophae rhamnoides leaf tea through solid-state fermentation of aspergillus cristatum (MK346334, NCBI) separated from Fuzhuan tea, and belongs to the field of deep processing of tea leaves. Comprising the following steps: 1) carrying out fixation, rolling, baking and fragrance extraction on freshly picked hippophae rhamnoides leaves to obtain hippophae rhamnoides green tea; 2) sterilizing the tea leaves to obtain sterile tea leaves; 3) inoculating 8% aspergillus cristatus spore suspension into the sterile tea leaves; and (4) carrying out solid-state fermentation at 28 DEG C for 8 days, and drying to obtain the eurotium cristatum and hippophae rhamnoides leaf tea. Results show that the sea-buckthorn green tea is fermented by aspergillus cristatus (MK346334, NCBI) separated from Fuzhuan tea, the color of the tea leaves is deepened, the leaf surfaces are'golden flower 'full, and the tea leaves have obvious fungus fragrance; tea soup is darkened in tawny color, and the content of total phenols and total flavonoids is increased; sensory evaluation results show that the eurotium cristatum and hippophae rhamnoides leaf tea is uniform and obvious in eurotium The tea soup is orange yellow, clear and bright; the taste is mellow and smooth, and the mushroom flavor is strong. According to the method, hippophae rhamnoides leaf resources can be fully utilized, and the eurotium cristatum hippophae rhamnoides leaf tea which is smooth in mouth feel and rich in fungus fragrance and The tea making and fermentation process is simple, the fermentation time is short, generation of active ingredients such as total phenols and total flavonoids in the hippophae rhamnoides leaves is promoted, and a feasible method is provided for effective utilization of hippophae rhamnoides leaf resources.

The invention relates to the technical field of preparation of sea-buckthorn peel residue degraded polysaccharide, in particular to sea-buckthorn peel residue degraded polysaccharide as well as a preparation method and application thereof. The sea buckthorn peel residue degraded polysaccharide comprises the following monosaccharides: mannose, glucosamine hydrochloride, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose. The method comprises the following steps: extracting and collecting hippophae rhamnoides fruit peel residue crude polysaccharide by taking hippophae rhamnoides fruit peel residue as a raw material through an ultrasonic extraction method, and then degrading the hippophae rhamnoides fruit peel residue crude polysaccharide by adopting a free radical degradation method based on an ascorbic acid-hydrogen peroxide system to obtain the degraded hippophae The prepared sea-buckthorn pericarp residue degraded polysaccharide has the effects of scavenging free radicals, inhibiting the activity of alpha-amylase and inhibiting the activity of alpha-glucosidase, and all the effects after degradation are remarkably improved.

The invention discloses application of a hippophae rhamnoides Hgfw3.2 gene in regulation and control of fruit size, and belongs to the technical field of biological genetic engineering. The nucleotide sequence of the Hgfw3.2 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the protein coded by the Hgfw3.2 gene is as shown in SEQ ID NO. 2. According to the invention, the key gene Hgfw3.2 for regulating and controlling the fruit size is cloned and identified from sea-buckthorn in Jiangcumin for the first time, and the positive regulation and control function of the gene in fruit development is defined. Through overexpression of the Hgfw3.2 gene in a tomato system, the weight, the longitudinal diameter and the transverse diameter of a fruit are obviously increased, the volume of the fruit is obviously increased, and the promotion effect of the gene on the size of the fruit is proved. Compared with the prior art, the invention provides a new gene resource from an extreme environment adaptive plant sea-buckthorn, enriches a gene pool for regulating and controlling the fruit size, and provides a new molecular target for improving the fruit size.

The invention relates to the technical field of hippophae rhamnoides powder, and discloses hippophae rhamnoides powder and a preparation method thereof.The method comprises the steps that hippophae rhamnoides fruits are cleaned, pulped and separated to obtain fruit juice and fruit residues, and then hippophae rhamnoides seeds and peel are separated from the fruit residues; sequentially carrying out heat pump mimicry drying, crushing, supercritical carbon dioxide degreasing and superfine crushing treatment on the sea-buckthorn peel; mixing the sea-buckthorn fruit juice with the degreased sea-buckthorn fruit peel powder according to a specific ratio, selectively adding auxiliary materials such as maltodextrin, emulsifying, homogenizing, and spray-drying to obtain the sea-buckthorn fruit powder. The hippophae rhamnoides fruit powder has hippophae rhamnoides fruit substance content (dry basis) of 50-70% and fiber content of 20-70%, and the hippophae rhamnoides fruit peel is used for replacing part or all of an exogenous drying aid, so that the natural nutritional ingredients and flavor of hippophae rhamnoides fruits are reserved, the dietary fiber content is increased, and the comprehensive utilization of the whole hippophae rhamnoides fruits is realized.

The application discloses a T5H gene from Hippophae rhamnoides and application of a protein coded by the T5H gene in improving cold resistance of plants, and belongs to the technical field of biotechnology. The T5H gene from Hippophae rhamnoides and the protein coded by the T5H gene can enhance survival rate and cold resistance of plants (such as crops) under low-temperature stress, and have wide application prospect and popularization value in agricultural production practice.

The utility model relates to the technical field of hippophae rhamnoides honey processing, in particular to a beater for hippophae rhamnoides honey processing, which comprises a base and a shell, the front side of the upper end of the base is fixedly connected with the shell, an auxiliary fruit pomace cleaning structure is arranged in the shell, and the lower end of a second box body is provided with a second discharge port. Through cooperation of an auxiliary fruit pomace cleaning structure and a fruit pomace cleaning structure, an output shaft of a first servo motor rotates to drive a cylinder to rotate, and the cylinder rotates to drive a roller to rotate, so that hippophae rhamnoides honey on a filter screen in a first box body can smoothly fall into a hippophae rhamnoides honey collecting barrel below; the nut rotates to enable the straight rod to rotate in the first box, so that the straight rod moves rightwards, the straight rod moves to drive the nut to move, when the end of the nut is far away from the disc, the disc rotates to drive the groove formed in the disc to rotate, the filter screen rotates along with the disc, and therefore an operator can conveniently clean fruit seeds and pulp residues on the filter screen.

The utility model relates to a hippophae rhamnoides cuttage tending device which comprises a cuttage box (1) and a water tank (2), the cuttage box (1) is arranged on the water tank (2), a plurality of water leakage holes (11) are formed in the box bottom of the cuttage box (1), a permeable sand isolation layer (12) is arranged in the cuttage box (1), a tending mechanism (3) is arranged above the cuttage box (1), the tending mechanism comprises a tending frame (31) and a track frame (32), the track frame (32) is installed on the cuttage box, and the water leakage holes (11) are formed in the water leakage holes (11). The tending frame (31) is installed on the track frame in a sliding mode, a plurality of spraying pipes (33) and a plurality of light supplementing lamps (34) are arranged on the tending frame, a plurality of layers of shading cloth (35) are arranged on the track frame, a water pump (4) is installed on the water tank (2), the water pump is connected with the spraying pipes, and the fish tank temperature adjusting device further comprises a control box (5) and a fish tank temperature adjusting heater (6). By using the device, humidity, temperature and illumination are convenient to control, and the hippophae rhamnoides hard branch rooting and survival are facilitated. The device is simple in structure and convenient to use.

The invention discloses a highland barley and fructus lycii polypeptide beverage as well as a preparation method and application of the highland barley and fructus lycii polypeptide beverage. The formula comprises the following components in parts by weight: 5-15 parts of highland barley extract, 3-10 parts of lycium ruthenicum primary pulp, 3-10 parts of hippophae rhamnoides primary pulp, 2-8 parts of radix puerariae extract, 1-5 parts of giant salamander active peptide powder, 1-4 parts of oyster extract, 0.5-3 parts of ginseng powder, 0.1-1 part of compound amino acid, 0.5-2 parts of a natural sweetening agent, 0.05-0.3 part of a stabilizer and the balance of water. The invention relates to the technical field of health-care food and beverage processing, and has the beneficial effects that Tibet Plateau characteristic resources are systematically compatible with marine active peptide giant salamander peptide, traditional anti-alcohol medicinal materials, functional amino acid and trace element yeast, and the combination acts on the whole process of absorption-metabolism-excretion of alcohol metabolism in a multi-target manner, so that the anti-alcohol effect is achieved. And the liver is synergistically protected through multiple ways of resisting oxidation, resisting inflammation, protecting liver cells and the like.

This invention discloses a gel with antibacterial and anti-inflammatory effects and its preparation method, belonging to the field of decontamination gel technology. The gel, by weight, comprises the following components: 10-25 parts of *Hippophae rhamnoides* flavonoid extract, 10-30 parts of *Hippophae rhamnoides* polysaccharide extract, 1-3 parts of gel matrix, 5-10 parts of humectant, 5-10 parts of surfactant, and 0.2-1 parts of preservative. This invention separates flavonoids and polysaccharides from *Hippophae rhamnoides* using ultrasound-assisted extraction technology and combines them with a gel matrix to form a stable hydrogel system. By optimizing the extraction process of active ingredients from *Hippophae rhamnoides* and combining it with a gel delivery system, this invention achieves synergistic antibacterial and anti-inflammatory effects of flavonoids and polysaccharides. The product has stable performance and high safety, suitable for clinical and home decontamination scenarios.

The invention discloses a preparation method for improving the stability of a sweetening agent in a sucrose-free hippophae rhamnoides beverage. The preparation method comprises the steps of graded pretreatment of hippophae rhamnoides raw materials, preparation of a sweetening agent compound system, multi-component stability regulation and control, gradient mixing and multi-stage homogenization, segmented sterilization and sterile filling. D-sodium erythorbate and tea polyphenol synergistically resist oxidation, sweetening agent is added in three times, three-stage homogenization optimization dispersion is carried out, pH is regulated in multiple links, the stability of the sweetening agent is improved, and the retention rate of sucralose is greater than or equal to 92% and the retention rate of erythritol is greater than or equal to 95% after storage at 25 DEG C in a dark place for 6 months. The process also can improve the clarity and taste of the beverage, control microbial contamination, retain the nutrients of the sea-buckthorn, is controllable in parameters, supports flexible replacement of the sweetening agent, reduces the production cost, conforms to the healthy and green development trend of sucrose-free beverages, and is suitable for industrial production.

The invention provides an external traditional Chinese medicine composition with hemostasis and wound healing effects, which relates to the field of traditional Chinese medicine compositions and comprises the following raw materials in parts by weight: 10-20 parts of bamboo shavings, 15-25 parts of rhododendron anthopogonoide, 10-20 parts of centella asiatica, 5-15 parts of lethariella cladonioides, 8-18 parts of physalis alkekengi, 10-20 parts of smoked plum, 12-22 parts of herba lycopi, 15-25 parts of sea-buckthorn, 10-20 parts of mung bean coat and 5-15 parts of asiatic toddalia root. The external traditional Chinese medicine composition can synchronously realize rapid hemostasis, heat clearing, detoxifying, blood activating, swelling subsiding, tissue regeneration promoting and sore healing.

The invention belongs to the technical field of polysaccharide preparation, and particularly discloses a hippophae rhamnoides fruit acid polysaccharide and a preparation method and application thereof.The hippophae rhamnoides fruit acid polysaccharide is hippophae rhamnoides fruit acid polysaccharide SPB1, and the hippophae rhamnoides fruit acid polysaccharide SPB1 comprises rhamnose, mannose, galacturonic acid, glucose, galactose and arabinose; the molar ratio of rhamnose to mannose to galacturonic acid to glucose to galactose to arabinose is 1: 1.05: 4.37: 1.44: 4.20: 29.28, and the molecular weight of the seabuckthorn fruit acidic polysaccharide SPB1 is 6.20 * 10 < 3 > kDa. According to the sea-buckthorn fruit acid polysaccharide and the preparation method and application thereof, the extraction method of the sea-buckthorn fruit polysaccharide is improved, the large-molecular-weight sea-buckthorn fruit acid polysaccharide can be extracted under the condition that the chemical structure of the polysaccharide is not damaged, the purity of the polysaccharide can be improved, and comprehensive utilization of the sea-buckthorn fruit polysaccharide is facilitated.