197 results about "Monosaccharide composition" patented technology

The invention provides homogenized pitaya polysaccharide as well as a preparation method and application thereof, and belongs to the technical field of polysaccharide extraction and purification. The homogenized pitaya polysaccharide is a polysaccharide of which the monosaccharide is composed of mannose, glucosamine hydrochloride, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose, the relative molecular weight is 8.08 * 10 < 4 > Da, and the corresponding molar ratio is (0.181): (0.250): (16.544): (12.762): (3.793): (0.739): (51.014): (14.716). The invention further provides a preparation method of the homogenized pitaya polysaccharide and application of the homogenized pitaya polysaccharide in preparation of lipid-lowering and sugar-lowering drugs. The homogenized pitaya polysaccharide disclosed by the invention has an obvious lipid-lowering and blood-glucose-reducing effect, is from a natural product, so that the homogenized pitaya polysaccharide has no toxic or side effect and can be used for preparing lipid-lowering and blood-glucose-reducing medicines.

The invention relates to the field of establishment of fingerprints of traditional Chinese medicinal materials, in particular to an establishment method and application of a salvia miltiorrhiza polysaccharide HPLC (High Performance Liquid Chromatography) fingerprint. The establishment method comprises the following steps: preparation of a test solution: taking a salvia miltiorrhiza polysaccharide hydrolysis solution, carrying out derivatization in a reaction bottle, and passing through a 0.1-0.3 [mu] m microporous filter membrane; preparing a reference substance solution, namely weighing mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose reference substances, and preparing into a mixed reference substance solution; performing HPLC (high performance liquid chromatography) determination; and establishing the salvia miltiorrhiza polysaccharide HPLC fingerprint spectrum. According to the method, the difference of monosaccharide composition and content in salvia miltiorrhiza medicinal materials from different producing areas and the spectrum-effect relationship between antioxidant activity and anticoagulant activity are clarified, and a certain reference basis is provided for quality control and evaluation of the salvia miltiorrhiza medicinal materials from the aspect of polysaccharide.

The invention discloses astragalus polysaccharide processed by different processing technologies and application thereof, self-weight polysaccharides of RAPSs, SAPSs, WAPSs and HAPSs are extracted from crude products and processed products obtained by salt processing, wine processing and honey processing, and the influence of different processing methods on the structure and bidirectional immunomodulatory activity of the astragalus polysaccharide is discussed. Results show that different processing methods change the properties and structural characteristics of the astragalus polysaccharide so as to influence the bidirectional immunomodulatory activity of the astragalus polysaccharide; the extraction rate, chemical composition, molecular weight, monosaccharide composition, chain conformation and surface morphology, Zeta potential and particle size, immunity enhancement and anti-inflammatory activity and the like of the four polysaccharides are remarkably different, but the four polysaccharides have the same typical crystal interior, main glycoside residues and O-glucosidic bonds. In a test concentration range, the honey-processed polysaccharide has a relatively strong immunity enhancing capability, and the wine-processed polysaccharide has a relatively strong anti-inflammatory capability.

The invention discloses a method for detecting the content of monosaccharide components in bacterial capsular polysaccharide or a conjugate thereof, and belongs to the field of biological product detection. Taking pneumococcus as an example, different hydrolysis methods are adopted for different serotypes of capsular polysaccharides, connected glucosidic bonds are broken through hydrolysis, released monosaccharide components and 1-phenyl-3-methyl-5-pyrazolone are subjected to derivatization under the alkaline condition to generate derivatives with ultraviolet absorption, and then the capsular polysaccharides with different serotypes are obtained. And then detecting the composition and the content of each monosaccharide generated by polysaccharide hydrolysis through liquid chromatography. According to the method, each monosaccharide component can be quickly and effectively hydrolyzed, meanwhile, various polysaccharides with different structural characteristics can be separated and detected by adopting a derivatization method, whether the monosaccharide composition of each serotype polysaccharide accords with a theoretical structure or not can be qualitatively analyzed, and the content of each monosaccharide component can be quantitatively determined to calculate the content of the polysaccharides; and a better technical support is provided for quality control of the antigen component capsular polysaccharide of the bacterial vaccine.

The application provides a Huangdache oligosaccharide with GLP-1 agonist activity and application thereof, and belongs to the technical field of medicines. 3 The oligosaccharide component ELYP-3 has a molecular weight of 3.2*10 3 Da, and the molar ratio of monosaccharides is rhamnose:galacturonic acid:glucose:galactose:arabinose = 1:32.42:45.37:9.11:6.54. The Huangdache oligosaccharide fragment obtained by glycosidase degradation has significant hypoglycemic and weight loss activities, the preparation process is simple, specific and green, and provides scientific basis and theoretical support for the development of natural GLP-1 agonists. Meanwhile, the functional oligosaccharide provides a wide application prospect for the development of medicines with blood glucose regulation.

The present invention discloses an anti-non-small cell lung cancer active birch inonotus polysaccharide IOP-1 and its application, belonging to the field of biomedicine. The present invention adopts a method of boiling water extraction and ethanol precipitation of birch inonotus polysaccharide, and combines DEAE cellulose column purification technology, and utilizes the principle that macromolecular polysaccharides are insoluble in organic solvents to successfully achieve the separation of birch inonotus polysaccharide and obtain a birch inonotus polysaccharide IOP-1 with anti-non-small cell lung cancer activity, wherein the monosaccharide composition of the birch inonotus polysaccharide IOP-1 includes fucose, rhamnose, arabinose, galactose, glucose, xylose and mannose with a molar mass ratio of 2.41:0.58:1.03:2.17:82.20:4.33:7.29. Its significant effect against non-small cell lung cancer was verified by in vitro and in vivo experiments, providing a new treatment method for the clinical treatment of non-small cell lung cancer.

The invention relates to the field of medicines, foods or health-care products, in particular to big-fruit hawthorn heteropolysaccharide as well as a preparation method and application thereof. The large-fruit hawthorn heteropolysaccharide is prepared from galacturonic acid, glucuronic acid, mannose, xylose, arabinose, glucose, galactose, fucose and rhamnose. The weight-average molecular weight of the big fruit hawthorn heteropolysaccharide is 81.6 kDa to 157.3 kDa. The product has unique structures of complex monosaccharide composition, low molecular weight, appropriate uronic acid content, low esterification degree and the like; compared with CP which is single in structure and high in esterification, the CP is better in oxidation resistance and bile acid binding capacity; compared with hawthorn polysaccharide with low uronic acid content and high molecular weight, the hawthorn polysaccharide has the advantages that the enzyme inhibition activity is obviously enhanced, and the hawthorn polysaccharide has a wide prospect in development of functional food, health care products or medicines for regulating blood sugar / blood fat, resisting oxidation and preventing metabolic syndromes.

The invention discloses a Zhejiang anoectochilus roxburghii polysaccharide and application thereof in preparation of cartilage injury repair medicine.The Zhejiang anoectochilus roxburghii polysaccharide is characterized in that a crude polysaccharide AZJP is extracted from the Zhejiang anoectochilus roxburghii polysaccharide through a hot water extraction method, an anion exchange column DEAE-celluse-52 and a gel permeation chromatographic column Sephadex G-200 are used for separating the AZJP, and the Zhejiang anoectochilus roxburghii homogeneous polysaccharides AZJP-1a, AZJP-2a, AZJP-2b and AZJP-2c are obtained; measuring UV (ultraviolet), IR (infrared), molecular weight, monosaccharide composition, methylated sugar residues, NMR (nuclear magnetic resonance) signals, triple helix structures, SEM (scanning electron microscope) and AFM (atomic force microscopy) surface microstructures and cartilage repair activity; the four homogeneous polysaccharides have remarkable cartilage repair activity on transgenic zebrafish, are superior to a positive drug alendronate sodium, and have application prospects in development of drugs for treating osteoarticular cartilage injuries.

The invention discloses emblic leafflower fruit polysaccharide with sugar-controlling and lipid-lowering activity as well as a preparation method and application of the emblic leafflower fruit polysaccharide. The polysaccharide is prepared by taking fresh phyllanthus emblica pulp as a raw material through hot water extraction, yeast fermentation, desorption after XAD-16 macroporous resin adsorption, alcohol precipitation, ultrafiltration and DEAE-52 cellulose column purification. The low-molecular-weight gulcomannan is separated from phyllanthus emblica for the first time, and monosaccharide composition of the gulcomannan mainly comprises glucose, mannose and galactose and contains trace rhamnose and xylose. The polysaccharide has sugar-controlling and lipid-lowering active action targets in the whole digestive tract (the upper digestive tract: glycolipid digestion and absorption enzymes alpha-amylase, alpha-glucosidase and pancreatic lipase inhibitory activity, starch hydrolysis delay, bile acid adsorption capacity and cholesterol micelle dissolution inhibition capacity; in the lower digestive tract, the multiplication promoting capacity of glycolipid metabolism-related probiotics) shows extremely high biological activity, and can be applied to food and health care products.

The invention belongs to the technical field of biochemistry, and particularly discloses dragon fruit residue polysaccharide as well as a preparation method and application thereof. The main structure of the pitaya residue polysaccharide is a repetitive unit composed of galactose and lactone galactose; in the monosaccharide composition of the pitaya residue polysaccharide, the content of galactose is 42 wt% or above. The pitaya pomace polysaccharide is prepared by taking pitaya pomace as a raw material and carrying out acidolysis, alcohol precipitation, dialysis and freeze drying on the pitaya pomace powder, and the pitaya pomace polysaccharide has high galactose content. A high-fat diet mouse model is used for testing the intestinal mucoprotein proportion, the oxidation resistance, the blood fat content and the like of the pitaya residue polysaccharide, and the result proves that the pitaya residue polysaccharide is beneficial to reducing blood fat, improving the oxidation resistance of the liver and maintaining the stability of mucus, has better protection and repair effects on intestinal barrier injury, and can be used for preparing the health-care food. The compound can be used for preparing medicines with the effects of reducing blood sugar, protecting liver, repairing intestinal barriers and the like.

The invention discloses a preparation method and application of roasted barley and malt polysaccharide with an anti-cancer effect. The roasted barley malt polysaccharide is a neutral heteropolysaccharide which mainly takes-> 3)-beta-D-Glcp-(1-> and->-beta-D-Glcp-(1->) as main chains, branches exist at O-3 / O-4 positions, and the branch chains are mainly composed of-> 4)-beta-D-Xylp-(1->,-> 3, 4)-beta-D-Xylp-(1-> and alpha-D-Glcp-(1->. The monosaccharide composition comprises arabinose, glucose and xylose in a molar mass ratio of 1.86: 33.43: 1.00, and the molecular weight is 23.81 kDa. In-vitro activity experiments prove that the roasted barley malt polysaccharide disclosed by the invention has a remarkable inhibition effect on proliferation of liver cancer cells, lung cancer cells, breast cancer cells and colorectal cancer cells, particularly on the liver cancer cells, and shows outstanding anti-tumor activity, so that the roasted barley malt polysaccharide is expected to be developed and applied in the fields of foods, health-care products or medicines.

The invention discloses inonotus obliquus polysaccharide IOP-1 capable of resisting non-small cell lung cancer activity and application, and belongs to the field of biological medicine. The method for extracting the inonotus obliquus polysaccharide by boiling water and precipitating the inonotus obliquus polysaccharide by ethanol is combined with a DEAE (diethylaminoethanol) cellulose column purification technology, and the principle that macromolecular polysaccharide is insoluble in an organic solvent is utilized, so that the separation of the inonotus obliquus polysaccharide is successfully realized, and the inonotus obliquus polysaccharide IOP-1 with non-small cell lung cancer resisting activity is obtained. The monosaccharide of the inonotus obliquus polysaccharide IOP-1 comprises fucose, rhamnose, arabinose, galactose, glucose, xylose and mannose, wherein the molar mass ratio of the fucose to the rhamnose to the arabinose to the galactose to the glucose to the xylose to the mannose is 2.41: 0.58: 1.03: 2.17: 82.20: 4.33: 7.29. In-vivo and in-vitro experiments prove that the traditional Chinese medicine composition has a remarkable effect of resisting the non-small cell lung cancer, and a novel treatment method is provided for clinical treatment of the non-small cell lung cancer.

The invention discloses a preparation method of cordyceps sobolifera polysaccharide and application of the cordyceps sobolifera polysaccharide in relieving acute chemical liver injury. Monosaccharide of the cordyceps sobolifera polysaccharide (abbreviated as CCPW) is composed of glucose, mannose and galactose according to the molar ratio of 8.918: 1: 0.356, and the relative molecular mass is 2.62 * 10 < 4 > Da. Efficacy experiments show that the cordyceps sobolifera homogeneous polysaccharide CCPW can significantly inhibit increase of ALT and AST in a CCl4-induced acute chemical liver injury model mouse, effectively improve the degree of liver cell disruption and swelling, relieve liver inflammatory response, relieve CCl4-induced mouse liver tissue injury, protect mouse intestinal barriers, recover intestinal microbial balance and have obvious liver protection activity. The cordyceps sobolifera polysaccharide CCPW is clear in composition and remarkable in effect, and has wide development and application prospects in the aspect of relieving acute chemical liver injury.

The invention firstly discloses application of portulaca oleracea polysaccharide in preparation of the medicine for treating psoriasis, the medicine for treating psoriasis can improve psoriasis-like skin injury and reduce skin tissue inflammation, and the portulaca oleracea polysaccharide is single-component pure polysaccharide extracted from portulaca oleracea and purified. The monosaccharide composition is composed of all or several monosaccharides of rhamnose, arabinose, galactose, glucose, xylose and galacturonic acid, and the molecular weight of the monosaccharide composition is 80-200 kDa. The invention further discloses application of the purslane polysaccharide in preparation of drugs for treating psoriasis and application of the purslane polysaccharide in preparation of cosmetics and medical auxiliary materials for relieving psoriasis-like skin injury. The purslane polysaccharide disclosed by the invention is high in purity, clear in structure and high in safety, has a good psoriasis treatment effect, is superior to dexamethasone in curative effect, can be used as a skin external medicine, a cosmetic, a medical instrument and a medical auxiliary material, and has various potential application values.

The invention discloses a construction method and application of a fucoidin fingerprint spectrum, monosaccharide composition of fucoidin is used as fingerprint spectrum research of index components, and ion chromatography fingerprint spectrums of different batches of fucoidin are constructed by optimizing an acid hydrolysis method and ion chromatography conditions. A theoretical basis can be provided for the quality control of the fucoidin. Meanwhile, the ion chromatograms of different algal polysaccharides are analyzed to find that different algal polysaccharides have differences, and reference is provided for quality control of fucoidin and algal polysaccharides from different sources.

The invention discloses stropharia rugoso-annulata polysaccharide as well as a preparation method and application thereof. The monosaccharide of the stropharia rugoso-annulata polysaccharide disclosed by the invention comprises glucose, galactose, mannose and galacturonic acid; the weight-average molecular weight of the stropharia rugoso-annulata polysaccharide is 1.4712 * 10 < 4 > to 1.623 * 10 < 4 > Da; the main chain of the stropharia rugoso-annulata polysaccharide contains-> 6)-alpha-D-(O-Me)-Galp-(1->, 1, 6-Glc, 1, 3-Man and 1.3-Gal exist in the main chain or a side chain, and terminal residues contain T-Glc and T-Gal. According to the invention, by constructing a DSS induced UC mouse model, the influence of Stropharia rugosoannulata polysaccharide (SRP1) on UC mice is investigated. Results show that the stropharia rugoso-annulata polysaccharide has a certain treatment effect on ulcerative colitis. In-vivo experiments show that the stropharia rugoso-annulata polysaccharide disclosed by the invention can increase abundance of bacteroides and thick-wall funguses and reduce abundance of proteobacteria and deferribacterium funguses; in-vitro experiments show that the stropharia rugoso-annulata polysaccharide can increase the abundance of beneficial bacteria bacteroides, reduce the abundance of harmful bacteria Escherichia-Shigella and Enterococcus, and increase the content of short-chain fatty acids.

The invention discloses a gymnadenia conopsea octasaccharide-decaose mixture as well as a preparation method and application thereof, and belongs to the technical field of medicines. The monosaccharide of the octasaccharide-decataccharide mixture of the gymnadenia conopsea comprises N-acetamino galactose, fucose, glucuronic acid or sugar alcohol thereof, galactose, N-acetylglucosamine, dehydrated talose and a sulfate group. The octasaccharide to decataccharide mixture of the gymnadenia conopsea disclosed by the invention can be used for remarkably prolonging the time of activating partial thromboplastin and prothrombin and effectively inhibiting endogenous and common coagulation pathways. The octasaccharide-decaose mixture of the gymnadenia conopsea can be used as a potential endogenous factor X enzyme inhibitor for preparing medicines for treating thrombotic cardiovascular diseases.

The invention discloses an extraction process and a quality control method of acanthopanax senticosus polysaccharide and application of the acanthopanax senticosus polysaccharide. The acanthopanax senticosus polysaccharide is prepared from acanthopanax senticosus roots, stems, leaves and fruits through a water extraction and alcohol precipitation extraction method. The polysaccharides of the roots, the stems, the leaves and the fruits of the acanthopanax senticosus all contain monosaccharides which comprise 5.0% to 50% of D-mannose, 0.7% to 6.0% of L-rhamnose, 4.0% to 33% of D-glucuronic acid, 6.0% to 69% of D-glucose, 13% to 40% of D-galactose and 4.0% to 18% of D-arabinose. The quality control method ensures the quality of the acanthopanax senticosus. A standard for controlling the acanthopanax senticosus polysaccharide is established by analyzing chemical components of the acanthopanax senticosus polysaccharide, content limitation of core components of the acanthopanax senticosus polysaccharide is added to the standard, and the polysaccharide prepared from the acanthopanax senticosus selected according to the range is used for judging polysaccharides of the acanthopanax senticosus and other plants and different parts of the acanthopanax senticosus. The method is good in reproducibility, accurate and reliable, and the acanthopanax senticosus polysaccharide and medicinal materials of different parts of acanthopanax senticosus can be rapidly and accurately identified through infrared characteristics and monosaccharide composition.

The invention discloses a gracilaria variegata low-molecular-weight polysaccharide GBP-1a, the monosaccharide is composed of 82.8% of galactose and 17.2% of glucose through structural analysis, the molecular weight of the uniform polysaccharide is 2887Da, and the polysaccharide has no toxicity to THP-1 mononuclear cells and THP-1 macrophages within a set concentration range through activity detection; and after the polysaccharide is subjected to immune training on THP-1 mononuclear cells and THP-1 macrophages, the immune response of the polysaccharide is obviously higher than that of cells which are not subjected to polysaccharide immune training, so that the training of the polysaccharide is preliminarily deduced to possibly enable the cells to generate acquired immunity similar to vaccines.

The invention belongs to the technical field of food microbial fermentation, and particularly relates to modified fucoidin, a preparation method thereof and application of the modified fucoidin in regulating an intestinal flora structure. The method comprises the following steps: inoculating a fermentation culture medium containing fucoidin with an activated bifidobacterium longum seed solution, carrying out anaerobic fermentation, taking a supernatant, desalting, and drying to prepare the modified fucoidin. The main chain structure and monosaccharide composition of the modified fucose fructose are unchanged, and the content of sulfuric acid groups of the modified fucose fructose is reduced by 25%-35% compared with that before modification. When the modified fucoidin is used for regulating the intestinal flora, the molecular weight of the modified fucoidin is reduced to 15% or below compared with that before modification, the proportion of the intestinal beneficial flora can be remarkably increased, the intestinal utilization rate of the modified fucoidin by the intestinal flora is increased, and a basis is provided for subsequent research and development of food for improving the intestinal beneficial flora.

The invention discloses low-molecular-weight enteromorpha polysaccharide with antioxidant activity and a preparation method of the low-molecular-weight enteromorpha polysaccharide, and belongs to the technical field of polysaccharide preparation. The preparation method comprises the following steps: taking an enteromorpha crude polysaccharide solution, adding H2O2 to enable the concentration of the enteromorpha crude polysaccharide solution to be 0.04-0.08 M, and degrading for 90-120 minutes under ultraviolet rays to obtain the low-molecular-weight enteromorpha polysaccharide, the low-molecular-weight enteromorpha polysaccharide is composed of monosaccharides of rhamnose, glucose, galactose, xylose, mannose and arabinose according to the ratio of 1.08: 1.00: 0.83: 0.58: 0.69: 0.06, the scavenging capacity and the iron reducing capacity of DPPH, ABTS and hydroxyl free radicals are improved, and the low-molecular-weight enteromorpha polysaccharide is obtained. The compound has excellent antioxidant activity and can be used as or used for preparing an antioxidant active preparation. The preparation method disclosed by the invention is efficient and stable, can be used for preparing the low-molecular-weight enteromorpha polysaccharide with lighter color, lower molecular weight and higher antioxidant activity, and has higher potential application value.

The invention discloses purified barley seedling polysaccharide as well as a preparation method and application thereof. The polysaccharide is prepared from six monosaccharides, namely rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 2.88: 3.39: 1.51: 1: 0.76: 3.2. The relative molecular weight is 254.09 kDa. A fermentation method is combined with an innovative purification process, so that the barley seedling polysaccharide monomer with high purity and uniform structure is obtained for the first time. Through a multi-dimensional characterization technology, fine structure characteristics such as monosaccharide composition, glucosidic bond connection mode and branching degree are systematically analyzed, and a reliable material basis is provided for establishing structure-function accurate association. The purified barley seedling polysaccharide has a remarkable effect of promoting intestinal peristalsis, not only lays a scientific foundation for deep processing of barley seedlings and research and development of related functional products, but also provides a new theoretical support for relieving intestinal peristalsis disorder, and has a wide application prospect.

The invention belongs to the technical field of ocean, and particularly relates to sea cucumber polysaccharide with an anti-colitis effect and application of the sea cucumber polysaccharide. According to the invention, the holothuria leucospilota polysaccharide H.leucospilota PS with a brand new structure is separated and purified from holothuria leucospilota, and the holothuria leucospilota polysaccharide H.leucospilota PS is composed of five monosaccharides, namely glucosamine, glucuronic acid, galactosamine, galactose and fucose. According to the present invention, by changing the intestinal microflora structure, the polysaccharide further regulates the generation of 3-Hydroxybutyrate, L-Meliic acid and Succinic semialdehydes, such that the intestinal energy metabolism is regulated, the intestinal microflora disorder and the energy metabolism disorder caused by the colitis are improved, and the drug resistance and the side effect caused by the long-term taking of the colitis treatment agent can be avoided;

The invention relates to the field of biological extraction, substance analysis, application and effect evaluation, in particular to an extraction method of polysaccharide of an overground part of chicory, the polysaccharide and a related application and evaluation method. The method comprises the following steps: providing an extraction method of the polysaccharide of the overground part of the chicory, carrying out reflux extraction, concentration, precipitation, centrifugation, washing and freeze-drying to obtain the polysaccharide, and carrying out molecular weight distribution and monosaccharide composition analysis on the polysaccharide, and the polysaccharide has antioxidant and anti-inflammatory activity, can be used for preparing products for protecting myocardial cells and the like, and has wide application prospects. The invention also discloses a composition containing the polysaccharide and an evaluation method for comparing the myocardial protection activity of the polysaccharide at different parts of the cichorium intybus. The application achieves the technical effects of efficiently extracting the polysaccharide of the overground part of the chicory, determining the characteristics and effects of the polysaccharide, providing a basis for development of products related to cardiovascular disease prevention and treatment, and providing a method for evaluating the myocardial protection activity of the polysaccharide of different parts of the chicory.

Hippophae rhamnoides peel polysaccharides, and a preparation method therefor and a use thereof. The hippophae rhamnoides peel polysaccharides comprise the following monosaccharides: rhamnose, mannose, galactose, glucose, galacturonic acid, and arabinose. The preparation method comprises: degreasing, extracting, concentrating and performing alcohol precipitation to obtain water-soluble crude polysaccharides; and then using a non-polar macroporous adsorption resin to perform purification to obtain hippophae rhamnoides peel polysaccharides. The yield and content of the polysaccharides are high. By using the non-polar microporous adsorption resin to replace a relatively costly DEAE-cellulose and Sephadex gel, active polysaccharides in the Hippophae rhamnoides peel are enriched, the yield and purity of the polysaccharides are also greatly improved, and the polysaccharides have low production costs and are used for industrial batch production. The hippophae rhamnoides peel polysaccharides have significant effects of resisting ulcerative colitis, and alleviating constipation, indigestion, and intestinal flora disorder.

The invention discloses tartary buckwheat vinasse polysaccharide with antioxidant activity and a preparation method of the tartary buckwheat vinasse polysaccharide, and belongs to the technical field of polysaccharide. The molecular weight of the tartary buckwheat vinasse polysaccharide with the antioxidant activity is 3.84 * 10 < 5 >, and the monosaccharide is prepared from Man-mannose, Rib-ribose, Rham-rhamnose, GlcUA-glucuronic acid, GalUA-galacturonic acid, Glc-glucose, Gal-galactose, Xyl-xylose and Ara-arabinose according to the molar ratio of (41.60): (20.20): (1.70): (2.84): (4.45): (8.90): (14.39): (6.27): (5.82). The tartary buckwheat vinasse polysaccharide is subjected to crude extraction through an ultrasonic-assisted water extraction and alcohol precipitation method, the extracted crude polysaccharide is purified through ion exchange column and sephadex column chromatography, and the prepared tartary buckwheat vinasse polysaccharide has a good scavenging effect on ABTS < + > free radicals, DPPH free radicals and hydroxyl free radicals and has antioxidant activity.

The invention belongs to the technical field of food processing, and particularly relates to low-molecular-weight fucoidin and application thereof. The fucoidin with low molecular weight is obtained after the fucoidin is treated, the number-average molecular weight of the fucoidin is 3-4 kDa, the content of sulfate radicals in the fucoidin is 10%-18%, the fucoidin has specific monosaccharide composition, and experimental results show that the fucoidin has the advantages that the fucoidin is low in content of sulfate radicals, and the fucoidin is low in content of sulfate radicals. The low-molecular-weight fucoidin provided by the invention has a wide application prospect in preparation of drugs for adjuvant treatment of thyroid nodules, and fills the technical blank about the relationship between fucoidin and thyroid nodules at present.

This invention belongs to the field of intestinal microbiome technology, specifically relating to a capsular polysaccharide derived from Bacteroides salorlis CSP6, its preparation method, and its applications. The monosaccharide composition of the capsular polysaccharide is mainly composed of fucose, glucose, and glucosamine, with a molecular weight of 7.139 × 10⁻⁶. 5 The strain described is deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC NO: M20221993. In vitro studies have found that the capsular polysaccharide derived from Bacteroides Salieris CSP6 can inhibit α-glucosidase; in vivo animal experiments have demonstrated that this capsular polysaccharide can inhibit the rise in postprandial blood glucose and has a hypoglycemic effect.

The invention discloses a polysaccharide component for inhibiting activity of alpha-glucosidase in the technical field of traditional Chinese medicine, the polysaccharide component is HCPN or HCPA1, the HCPN is mainly heteropolysaccharide composed of four monosaccharides, and the monosaccharides comprise Man, Glc, Gal and Ara; the HCPA1 is heteropolysaccharide mainly composed of seven monosaccharides, and the monosaccharides of the HCPA1 comprise Man, GlcA, Rha, GalA, Glc, Gal and Ara. The alpha-glucosidase inhibition ratio of HCPN and HCPA1 is measured, and the result shows that the alpha-glucosidase inhibition ratio of HCPN and HCPA1 is higher than that of a positive drug acarbose.

The present invention provides a polysaccharide that has a protective effect on kidney damage. The polysaccharide of the present invention is obtained from the receptacle of Podocarpus carylus or Podocarpus brevifolia, plants of the Podocarpaceae family, after ultrasonic extraction, distilled water reheating extraction, reduced pressure concentration, ethanol precipitation, and freeze-drying. According to content determination, the mass percentage of the polysaccharide of the present invention is greater than or equal to 50% in terms of glucose. The polysaccharide is composed of 7 monosaccharides, including mannose, rhamnose, glucose, galactose, xylose, arabinose and L-fucose. In vivo experiments on mice have confirmed that the polysaccharide of the present invention has a good protective effect on kidney damage caused by cisplatin. The polysaccharide of the present invention can be used to prepare medicines with a protective effect on kidney damage.