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154 results about "Monosaccharide composition" patented technology

Monosaccharides are the simplest unit of carbohydrates. They're composed of carbon, hydrogen, and oxygen atoms, and they cannot be broken down further since they are already in their simplest form. Their general formula is (CH2O)n, where n is any number equal or greater than 3.

Astragalus polysaccharide processed by different processing technologies and application thereof

The invention discloses astragalus polysaccharide processed by different processing technologies and application thereof, self-weight polysaccharides of RAPSs, SAPSs, WAPSs and HAPSs are extracted from crude products and processed products obtained by salt processing, wine processing and honey processing, and the influence of different processing methods on the structure and bidirectional immunomodulatory activity of the astragalus polysaccharide is discussed. Results show that different processing methods change the properties and structural characteristics of the astragalus polysaccharide so as to influence the bidirectional immunomodulatory activity of the astragalus polysaccharide; the extraction rate, chemical composition, molecular weight, monosaccharide composition, chain conformation and surface morphology, Zeta potential and particle size, immunity enhancement and anti-inflammatory activity and the like of the four polysaccharides are remarkably different, but the four polysaccharides have the same typical crystal interior, main glycoside residues and O-glucosidic bonds. In a test concentration range, the honey-processed polysaccharide has a relatively strong immunity enhancing capability, and the wine-processed polysaccharide has a relatively strong anti-inflammatory capability.
Owner:LANZHOU INSTITUTE OF CHEMICAL PHYSICS CHINESE ACADEMY OF SCIENCES +1

Xanthan oligosaccharides having glp-1 agonist activity and uses thereof

The application provides a Huangdache oligosaccharide with GLP-1 agonist activity and application thereof, and belongs to the technical field of medicines. 3 The oligosaccharide component ELYP-3 has a molecular weight of 3.2*10 3 Da, and the molar ratio of monosaccharides is rhamnose:galacturonic acid:glucose:galactose:arabinose = 1:32.42:45.37:9.11:6.54. The Huangdache oligosaccharide fragment obtained by glycosidase degradation has significant hypoglycemic and weight loss activities, the preparation process is simple, specific and green, and provides scientific basis and theoretical support for the development of natural GLP-1 agonists. Meanwhile, the functional oligosaccharide provides a wide application prospect for the development of medicines with blood glucose regulation.
Owner:ANHUI AGRICULTURAL UNIVERSITY

An active Inonotus obliquus polysaccharide IOP-1 against non-small cell lung cancer and its application

The present invention discloses an anti-non-small cell lung cancer active birch inonotus polysaccharide IOP-1 and its application, belonging to the field of biomedicine. The present invention adopts a method of boiling water extraction and ethanol precipitation of birch inonotus polysaccharide, and combines DEAE cellulose column purification technology, and utilizes the principle that macromolecular polysaccharides are insoluble in organic solvents to successfully achieve the separation of birch inonotus polysaccharide and obtain a birch inonotus polysaccharide IOP-1 with anti-non-small cell lung cancer activity, wherein the monosaccharide composition of the birch inonotus polysaccharide IOP-1 includes fucose, rhamnose, arabinose, galactose, glucose, xylose and mannose with a molar mass ratio of 2.41:0.58:1.03:2.17:82.20:4.33:7.29. Its significant effect against non-small cell lung cancer was verified by in vitro and in vivo experiments, providing a new treatment method for the clinical treatment of non-small cell lung cancer.
Owner:CHANGCHUN UNIV OF CHINESE MEDICINE

Hawthorn heteropolysaccharide as well as preparation method and application thereof

The invention relates to the field of medicines, foods or health-care products, in particular to big-fruit hawthorn heteropolysaccharide as well as a preparation method and application thereof. The large-fruit hawthorn heteropolysaccharide is prepared from galacturonic acid, glucuronic acid, mannose, xylose, arabinose, glucose, galactose, fucose and rhamnose. The weight-average molecular weight of the big fruit hawthorn heteropolysaccharide is 81.6 kDa to 157.3 kDa. The product has unique structures of complex monosaccharide composition, low molecular weight, appropriate uronic acid content, low esterification degree and the like; compared with CP which is single in structure and high in esterification, the CP is better in oxidation resistance and bile acid binding capacity; compared with hawthorn polysaccharide with low uronic acid content and high molecular weight, the hawthorn polysaccharide has the advantages that the enzyme inhibition activity is obviously enhanced, and the hawthorn polysaccharide has a wide prospect in development of functional food, health care products or medicines for regulating blood sugar / blood fat, resisting oxidation and preventing metabolic syndromes.
Owner:GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI +1

Phyllanthus emblica polysaccharide with sugar-controlling and lipid-lowering activity as well as preparation method and application of phyllanthus emblica polysaccharide

The invention discloses emblic leafflower fruit polysaccharide with sugar-controlling and lipid-lowering activity as well as a preparation method and application of the emblic leafflower fruit polysaccharide. The polysaccharide is prepared by taking fresh phyllanthus emblica pulp as a raw material through hot water extraction, yeast fermentation, desorption after XAD-16 macroporous resin adsorption, alcohol precipitation, ultrafiltration and DEAE-52 cellulose column purification. The low-molecular-weight gulcomannan is separated from phyllanthus emblica for the first time, and monosaccharide composition of the gulcomannan mainly comprises glucose, mannose and galactose and contains trace rhamnose and xylose. The polysaccharide has sugar-controlling and lipid-lowering active action targets in the whole digestive tract (the upper digestive tract: glycolipid digestion and absorption enzymes alpha-amylase, alpha-glucosidase and pancreatic lipase inhibitory activity, starch hydrolysis delay, bile acid adsorption capacity and cholesterol micelle dissolution inhibition capacity; in the lower digestive tract, the multiplication promoting capacity of glycolipid metabolism-related probiotics) shows extremely high biological activity, and can be applied to food and health care products.
Owner:SOUTH CHINA UNIV OF TECH

Barley-roasted malt polysaccharide with anti-cancer effect as well as preparation method and application of barley-roasted malt polysaccharide

The invention discloses a preparation method and application of roasted barley and malt polysaccharide with an anti-cancer effect. The roasted barley malt polysaccharide is a neutral heteropolysaccharide which mainly takes-> 3)-beta-D-Glcp-(1-> and->-beta-D-Glcp-(1->) as main chains, branches exist at O-3 / O-4 positions, and the branch chains are mainly composed of-> 4)-beta-D-Xylp-(1->,-> 3, 4)-beta-D-Xylp-(1-> and alpha-D-Glcp-(1->. The monosaccharide composition comprises arabinose, glucose and xylose in a molar mass ratio of 1.86: 33.43: 1.00, and the molecular weight is 23.81 kDa. In-vitro activity experiments prove that the roasted barley malt polysaccharide disclosed by the invention has a remarkable inhibition effect on proliferation of liver cancer cells, lung cancer cells, breast cancer cells and colorectal cancer cells, particularly on the liver cancer cells, and shows outstanding anti-tumor activity, so that the roasted barley malt polysaccharide is expected to be developed and applied in the fields of foods, health-care products or medicines.
Owner:SHANGHAI JIAOTONG UNIV +1

Preparation method of cordyceps sobolifera polysaccharide and application of cordyceps sobolifera polysaccharide in relieving acute chemical liver injury

The invention discloses a preparation method of cordyceps sobolifera polysaccharide and application of the cordyceps sobolifera polysaccharide in relieving acute chemical liver injury. Monosaccharide of the cordyceps sobolifera polysaccharide (abbreviated as CCPW) is composed of glucose, mannose and galactose according to the molar ratio of 8.918: 1: 0.356, and the relative molecular mass is 2.62 * 10 < 4 > Da. Efficacy experiments show that the cordyceps sobolifera homogeneous polysaccharide CCPW can significantly inhibit increase of ALT and AST in a CCl4-induced acute chemical liver injury model mouse, effectively improve the degree of liver cell disruption and swelling, relieve liver inflammatory response, relieve CCl4-induced mouse liver tissue injury, protect mouse intestinal barriers, recover intestinal microbial balance and have obvious liver protection activity. The cordyceps sobolifera polysaccharide CCPW is clear in composition and remarkable in effect, and has wide development and application prospects in the aspect of relieving acute chemical liver injury.
Owner:安徽虫草源生物科技有限公司 +1

Construction method and application of fucoidin fingerprint spectrum

The invention discloses a construction method and application of a fucoidin fingerprint spectrum, monosaccharide composition of fucoidin is used as fingerprint spectrum research of index components, and ion chromatography fingerprint spectrums of different batches of fucoidin are constructed by optimizing an acid hydrolysis method and ion chromatography conditions. A theoretical basis can be provided for the quality control of the fucoidin. Meanwhile, the ion chromatograms of different algal polysaccharides are analyzed to find that different algal polysaccharides have differences, and reference is provided for quality control of fucoidin and algal polysaccharides from different sources.
Owner:THIRD INSTITUTE OF OCEANOGRAPHY STATE OCEANI C ADMINISTRATION

Stropharia rugoso-annulata polysaccharide as well as preparation method and application thereof

The invention discloses stropharia rugoso-annulata polysaccharide as well as a preparation method and application thereof. The monosaccharide of the stropharia rugoso-annulata polysaccharide disclosed by the invention comprises glucose, galactose, mannose and galacturonic acid; the weight-average molecular weight of the stropharia rugoso-annulata polysaccharide is 1.4712 * 10 < 4 > to 1.623 * 10 < 4 > Da; the main chain of the stropharia rugoso-annulata polysaccharide contains-> 6)-alpha-D-(O-Me)-Galp-(1->, 1, 6-Glc, 1, 3-Man and 1.3-Gal exist in the main chain or a side chain, and terminal residues contain T-Glc and T-Gal. According to the invention, by constructing a DSS induced UC mouse model, the influence of Stropharia rugosoannulata polysaccharide (SRP1) on UC mice is investigated. Results show that the stropharia rugoso-annulata polysaccharide has a certain treatment effect on ulcerative colitis. In-vivo experiments show that the stropharia rugoso-annulata polysaccharide disclosed by the invention can increase abundance of bacteroides and thick-wall funguses and reduce abundance of proteobacteria and deferribacterium funguses; in-vitro experiments show that the stropharia rugoso-annulata polysaccharide can increase the abundance of beneficial bacteria bacteroides, reduce the abundance of harmful bacteria Escherichia-Shigella and Enterococcus, and increase the content of short-chain fatty acids.
Owner:HENAN AGRICULTURAL UNIVERSITY +2

Gracilaria heteroclada low-molecular-weight polysaccharide as well as preparation method and application thereof

The invention discloses a gracilaria variegata low-molecular-weight polysaccharide GBP-1a, the monosaccharide is composed of 82.8% of galactose and 17.2% of glucose through structural analysis, the molecular weight of the uniform polysaccharide is 2887Da, and the polysaccharide has no toxicity to THP-1 mononuclear cells and THP-1 macrophages within a set concentration range through activity detection; and after the polysaccharide is subjected to immune training on THP-1 mononuclear cells and THP-1 macrophages, the immune response of the polysaccharide is obviously higher than that of cells which are not subjected to polysaccharide immune training, so that the training of the polysaccharide is preliminarily deduced to possibly enable the cells to generate acquired immunity similar to vaccines.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

Low-molecular-weight enteromorpha polysaccharide with antioxidant activity and preparation method thereof

The invention discloses low-molecular-weight enteromorpha polysaccharide with antioxidant activity and a preparation method of the low-molecular-weight enteromorpha polysaccharide, and belongs to the technical field of polysaccharide preparation. The preparation method comprises the following steps: taking an enteromorpha crude polysaccharide solution, adding H2O2 to enable the concentration of the enteromorpha crude polysaccharide solution to be 0.04-0.08 M, and degrading for 90-120 minutes under ultraviolet rays to obtain the low-molecular-weight enteromorpha polysaccharide, the low-molecular-weight enteromorpha polysaccharide is composed of monosaccharides of rhamnose, glucose, galactose, xylose, mannose and arabinose according to the ratio of 1.08: 1.00: 0.83: 0.58: 0.69: 0.06, the scavenging capacity and the iron reducing capacity of DPPH, ABTS and hydroxyl free radicals are improved, and the low-molecular-weight enteromorpha polysaccharide is obtained. The compound has excellent antioxidant activity and can be used as or used for preparing an antioxidant active preparation. The preparation method disclosed by the invention is efficient and stable, can be used for preparing the low-molecular-weight enteromorpha polysaccharide with lighter color, lower molecular weight and higher antioxidant activity, and has higher potential application value.
Owner:OCEAN UNIV OF CHINA

Purified barley seedling polysaccharide as well as preparation method and application thereof

The invention discloses purified barley seedling polysaccharide as well as a preparation method and application thereof. The polysaccharide is prepared from six monosaccharides, namely rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 2.88: 3.39: 1.51: 1: 0.76: 3.2. The relative molecular weight is 254.09 kDa. A fermentation method is combined with an innovative purification process, so that the barley seedling polysaccharide monomer with high purity and uniform structure is obtained for the first time. Through a multi-dimensional characterization technology, fine structure characteristics such as monosaccharide composition, glucosidic bond connection mode and branching degree are systematically analyzed, and a reliable material basis is provided for establishing structure-function accurate association. The purified barley seedling polysaccharide has a remarkable effect of promoting intestinal peristalsis, not only lays a scientific foundation for deep processing of barley seedlings and research and development of related functional products, but also provides a new theoretical support for relieving intestinal peristalsis disorder, and has a wide application prospect.
Owner:YANCHENG INST OF TECH

Extraction method of polysaccharide of overground part of cichorium intybus, polysaccharide and related application and evaluation method

The invention relates to the field of biological extraction, substance analysis, application and effect evaluation, in particular to an extraction method of polysaccharide of an overground part of chicory, the polysaccharide and a related application and evaluation method. The method comprises the following steps: providing an extraction method of the polysaccharide of the overground part of the chicory, carrying out reflux extraction, concentration, precipitation, centrifugation, washing and freeze-drying to obtain the polysaccharide, and carrying out molecular weight distribution and monosaccharide composition analysis on the polysaccharide, and the polysaccharide has antioxidant and anti-inflammatory activity, can be used for preparing products for protecting myocardial cells and the like, and has wide application prospects. The invention also discloses a composition containing the polysaccharide and an evaluation method for comparing the myocardial protection activity of the polysaccharide at different parts of the cichorium intybus. The application achieves the technical effects of efficiently extracting the polysaccharide of the overground part of the chicory, determining the characteristics and effects of the polysaccharide, providing a basis for development of products related to cardiovascular disease prevention and treatment, and providing a method for evaluating the myocardial protection activity of the polysaccharide of different parts of the chicory.
Owner:HEBEI JINMU PHARM GRP CO LTD

Hippophae rhamnoides peel polysaccharides, and preparation method therefor and use thereof

Hippophae rhamnoides peel polysaccharides, and a preparation method therefor and a use thereof. The hippophae rhamnoides peel polysaccharides comprise the following monosaccharides: rhamnose, mannose, galactose, glucose, galacturonic acid, and arabinose. The preparation method comprises: degreasing, extracting, concentrating and performing alcohol precipitation to obtain water-soluble crude polysaccharides; and then using a non-polar macroporous adsorption resin to perform purification to obtain hippophae rhamnoides peel polysaccharides. The yield and content of the polysaccharides are high. By using the non-polar microporous adsorption resin to replace a relatively costly DEAE-cellulose and Sephadex gel, active polysaccharides in the Hippophae rhamnoides peel are enriched, the yield and purity of the polysaccharides are also greatly improved, and the polysaccharides have low production costs and are used for industrial batch production. The hippophae rhamnoides peel polysaccharides have significant effects of resisting ulcerative colitis, and alleviating constipation, indigestion, and intestinal flora disorder.
Owner:SHAANXI HAIXIANG PLATEAU BIOTECHNOLOGY CO LTD

Low-molecular-weight fucoidin and application thereof

The invention belongs to the technical field of food processing, and particularly relates to low-molecular-weight fucoidin and application thereof. The fucoidin with low molecular weight is obtained after the fucoidin is treated, the number-average molecular weight of the fucoidin is 3-4 kDa, the content of sulfate radicals in the fucoidin is 10%-18%, the fucoidin has specific monosaccharide composition, and experimental results show that the fucoidin has the advantages that the fucoidin is low in content of sulfate radicals, and the fucoidin is low in content of sulfate radicals. The low-molecular-weight fucoidin provided by the invention has a wide application prospect in preparation of drugs for adjuvant treatment of thyroid nodules, and fills the technical blank about the relationship between fucoidin and thyroid nodules at present.
Owner:SHANDONG XIAOYING BIOTECHNOLOGY CO LTD +1

A capsular polysaccharide derived from Bacteroides saurischia CSP6, its preparation method and application

This invention belongs to the field of intestinal microbiome technology, specifically relating to a capsular polysaccharide derived from Bacteroides salorlis CSP6, its preparation method, and its applications. The monosaccharide composition of the capsular polysaccharide is mainly composed of fucose, glucose, and glucosamine, with a molecular weight of 7.139 × 10⁻⁶. 5 The strain described is deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC NO: M20221993. In vitro studies have found that the capsular polysaccharide derived from Bacteroides Salieris CSP6 can inhibit α-glucosidase; in vivo animal experiments have demonstrated that this capsular polysaccharide can inhibit the rise in postprandial blood glucose and has a hypoglycemic effect.
Owner:OCEAN UNIV OF CHINA

Polysaccharide component for inhibiting activity of alpha-glucosidase as well as preparation method and application of polysaccharide component

The invention discloses a polysaccharide component for inhibiting activity of alpha-glucosidase in the technical field of traditional Chinese medicine, the polysaccharide component is HCPN or HCPA1, the HCPN is mainly heteropolysaccharide composed of four monosaccharides, and the monosaccharides comprise Man, Glc, Gal and Ara; the HCPA1 is heteropolysaccharide mainly composed of seven monosaccharides, and the monosaccharides of the HCPA1 comprise Man, GlcA, Rha, GalA, Glc, Gal and Ara. The alpha-glucosidase inhibition ratio of HCPN and HCPA1 is measured, and the result shows that the alpha-glucosidase inhibition ratio of HCPN and HCPA1 is higher than that of a positive drug acarbose.
Owner:AFFILIATED HOSPITAL OF ZUNYI UNIV

A polysaccharide with protective effects against kidney damage

The present invention provides a polysaccharide that has a protective effect on kidney damage. The polysaccharide of the present invention is obtained from the receptacle of Podocarpus carylus or Podocarpus brevifolia, plants of the Podocarpaceae family, after ultrasonic extraction, distilled water reheating extraction, reduced pressure concentration, ethanol precipitation, and freeze-drying. According to content determination, the mass percentage of the polysaccharide of the present invention is greater than or equal to 50% in terms of glucose. The polysaccharide is composed of 7 monosaccharides, including mannose, rhamnose, glucose, galactose, xylose, arabinose and L-fucose. In vivo experiments on mice have confirmed that the polysaccharide of the present invention has a good protective effect on kidney damage caused by cisplatin. The polysaccharide of the present invention can be used to prepare medicines with a protective effect on kidney damage.
Owner:GUANGXI MEDICAL UNIVERSITY

A sulfated arabinogalactan derived from *Streptococcus linearis*, its preparation method and application

This invention discloses an arabinogalactan sulfate derived from *Streptococcus filamentosa*, its preparation method, and its applications, belonging to the fields of natural product chemistry and marine biological resource development technology. The technical solution includes an arabinogalactan sulfate derived from *Streptococcus filamentosa*, with a monosaccharide composition comprising arabinose, galactose, and rhamnose, the molar percentage of arabinose being not less than 70%; the main chain backbone is modified with natural sulfate groups, with the sulfate groups replacing at least one hydroxyl site of the arabinose residues. This invention, when applied to anticoagulation, exhibits good safety and a significant anticoagulant effect.
Owner:SHANDONG ACAD OF MARINE SCI (QINGDAO NAT MARINE SCI RES CENT)

A shoumei polysaccharide, a preparation method and application thereof

This invention provides a Shoumei polysaccharide, its preparation method, and its application, belonging to the field of biomedical technology. The Shoumei polysaccharide is a homogeneous polysaccharide with the repeating sequence →4)-α-D-Gal. p A‑(1→, →4)‑β‑D‑Gal p ‑(1→,→4,6)‑β‑D‑Gal p ‑(1→,→6)‑β‑D‑Gal p -(1→、α-L-Ara f ‑(1→,→5)‑α‑L‑Ara f ‑(1→,→4)‑β‑D‑Glc p -(1→; The molecular weight of the Shoumei polysaccharide is 1100 kDa; The monosaccharide composition of the Shoumei polysaccharide is arabinose, galactose, glucose and galacturonic acid; The molar ratio of arabinose, galactose, glucose and galacturonic acid is 10.9:12.1:4.8:72.2, and the degree of galacturonic acid esterification is 33%. The Shoumei polysaccharide of the present invention can treat and / or prevent depression.
Owner:ANHUI AGRICULTURAL UNIVERSITY

Application of Uncaria rhynchophylla neutral polysaccharide URP-W in the preparation of anti-glioma drugs

ActiveCN121005799Bachieve separationSolve the technical problems of extraction and separationOrganic active ingredientsNervous disorderCelluloseMonosaccharide composition
This invention discloses the application of Uncaria rhynchophylla neutral polysaccharide URP-W in the preparation of anti-glioma drugs, belonging to the field of anti-tumor technology of medicinal plant polysaccharides. The invention obtains a crude polysaccharide extract from Uncaria rhynchophylla through hot water extraction and ethanol precipitation, followed by purification using a DEAE cellulose column and G200 dextran gel to obtain Uncaria rhynchophylla neutral polysaccharide URP-W. The monosaccharide composition of URP-W includes arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid, and mannuronic acid in a molar ratio of 17.99:6.32:21.71:27.17:2.56:7.57:16.20:0.49. Experiments have shown that URP-W can inhibit the proliferation of gliomas and induce apoptosis, exhibiting significant anti-tumor activity against glioma cells, providing a new drug raw material and treatment method for the clinical treatment of gliomas.
Owner:CHANGCHUN UNIV OF CHINESE MEDICINE

Armillariella mycelium polysaccharide with effect of improving cognitive impairment as well as preparation method and application of armillariella mycelium polysaccharide

The invention discloses armillariella mycelium polysaccharide ATMP capable of improving cognitive impairment and a preparation method and application of the armillariella mycelium polysaccharide ATMP. The polysaccharide is composed of two components, and the molecular weights of the two components are 2.30 * 10 < 4 > Da and 4.56 * 10 < 5 > Da respectively; the monosaccharide composition comprises mannose (Man), glucose (Glc) and galactose (Gal), and the molar ratio of the mannose (Man) to the glucose (Glc) to the galactose (Gal) is 1: 16.64: 1.96. Animal experiment results show that the armillariella mycelium polysaccharide ATMP can improve cognitive impairment induced by lipopolysaccharide (LPS) by improving the spatial memory ability of mice, repairing the disorder state and deletion injury of neurons in a hippocampal region and relieving neuroinflammatory response, so that the obvious neuroprotective effect is achieved. As a natural brain-benefiting active component, the armillariella tabescens mycelium polysaccharide ATMP has a wide application prospect in the related fields of brain health.
Owner:ANHUI UNIV +1

Wheat bran araboxylan and application thereof

The invention belongs to the technical field of biological medicine, and particularly relates to wheat bran araboxylan and application thereof. The invention provides wheat bran AX with specific molecular weight and monosaccharide composition. The wheat bran AX comprises the following monosaccharides: 0.20%-0.22% of glucuronic acid, 0.58%-0.62% of galacturonic acid, 0.21%-0.23% of rhamnose, 0.6%-0.7% of glucose, 1.4%-1.5% of galactose, 18%-19.2% of xylose and 21%-23% of arabinose. Compared with commercially available wheat bran AX, inulin, fructo-oligosaccharide and other common dietary fibers, the self-made wheat bran AX has higher diversity of microbial communities in the fermentation process, has a better intestinal flora regulating effect, further has a good relieving effect on the abnormal glucose and lipid metabolism of the body, and can be used for preventing and treating the abnormal glucose and lipid metabolism of the body. Therefore, the traditional Chinese medicine composition has a good regulation effect on various diseases such as non-alcoholic fatty liver and the like caused by abnormal glucose and lipid metabolism of the organism.
Owner:SHANDONG ACADEMY OF AGRICULTURAL SCIENCES +1

Nuclear magnetic resonance hydrogen spectrum method for directly analyzing high molecular weight hyaluronic acid structure and quantitative monomer composition

The invention discloses a nuclear magnetic resonance hydrogen spectrum method for directly analyzing a high molecular weight hyaluronic acid structure and quantitative monomer composition. The method comprises the following steps: S1, dissolving a high-molecular-weight hyaluronic acid sample in D2O, adding NaOD, and uniformly mixing to obtain a sample solution; the concentration of the hyaluronic acid sample in the D2O is 0.5 to 2 mg / mL; the final concentration of the NaOD in the sample solution is 75 mmol / L; s2, performing a nuclear magnetic resonance hydrogen spectrum test on the sample solution to obtain a nuclear magnetic resonance hydrogen spectrum spectrogram; and S3, obtaining an integral area proportion of a hydrogen atom characteristic peak from the nuclear magnetic resonance hydrogen spectrum so as to directly quantify the composition molar ratio of the two monomers of the high-molecular-weight hyaluronic acid. The method provided by the invention not only can realize direct structural characterization of the high molecular weight HA, but also can realize direct quantification of the composition proportion of the two monosaccharides, and the quantitative result is high in precision and reproducibility.
Owner:TSINGHUA UNIVERSITY

Kudouzi polysaccharide, and preparation method and application thereof

The application discloses Ixeris sonchifolia Hance polysaccharides, a preparation method and application thereof, belongs to the technical field of plant polysaccharides, and provides polysaccharides ISP-1a and ISP-2a, wherein the ISP-1a has a molecular weight of 2086 Da and is composed of seven monosaccharides of mannose, rhamnose, glucuronic acid, glucose, galactose, xylose and arabinose; the ISP-2a has a molecular weight of 1781 Da and is composed of five monosaccharides of mannose, rhamnose, galacturonic acid, xylose and arabinose. The whole grass of Ixeris sonchifolia Hance is subjected to hot water extraction after ethanol degreasing, neutral enzyme + Sevage method is used to remove protein, macroporous resin is used for decolorization, column chromatography elution is carried out, purification is carried out, and two kinds of uniform polysaccharides are obtained after freeze-drying. The polysaccharides ISP-1a and ISP-2a have good antioxidant and lipid-lowering effects, and can be applied to the preparation of food, health products or medicines with antioxidant and lipid-lowering functions.
Owner:SHENYANG PHARMA UNIV

Radix sophorae tonkinensis polysaccharide STGP-1, preparation method thereof and pharmaceutical use for treating lung squamous carcinoma

The application discloses radix sophorae tonkinensis polysaccharide STGP-1, a preparation method thereof and pharmaceutical use for treating lung squamous carcinoma, and relates to the technical field of medicines. The monosaccharide composition of STGP-1 includes fucose Fuc, arabinose Ara, galactose Gal, glucose Glc, xylose Xyl and mannose Man, and the molar ratio is 0.34:12.15:25.76:57.07:1.06:3.62. The STGP-1 is obtained by extraction, separation and purification from dried roots and rhizomes of radix sophorae tonkinensis, and the preparation method comprises the steps of pretreatment and crude extraction, impurity removal treatment, anion exchange column chromatography separation, gel column chromatography purification and the like. The STGP-1 can be used alone or in combination with anti-PD-1 monoclonal antibody for preparing a medicine for treating lung squamous carcinoma. The preparation process is stable, the source is natural, and the STGP-1 can provide a new high-efficiency and low-toxicity candidate medicine for immune combined treatment of lung squamous carcinoma.
Owner:GUANGXI MEDICAL UNIVERSITY

Caulis spatholobi polysaccharide, preparation method thereof and application of caulis spatholobi polysaccharide in preparation of medicine for treating inflammation

The invention belongs to the technical field of plant polysaccharide, and particularly discloses caulis spatholobi polysaccharide, a preparation method thereof and application of the caulis spatholobi polysaccharide in preparation of medicines for treating inflammation. Monosaccharide in the caulis spatholobi polysaccharide disclosed by the invention comprises mannose, glucuronic acid, galacturonic acid, glucose and arabinose in a molar ratio of (0.5-0.6): (0.3-0.5): (1-2): (1-2): (0.5-1.5). The caulis spatholobi polysaccharide provided by the invention is a novel uniform-structure polysaccharide which is separated from caulis spatholobi rattan for the first time, has a slightly acidic pH value, definite monosaccharide composition, definite molecular weight and clear glycosidic bond linking mode, has an anti-inflammatory effect, and is expected to be developed into a new anti-inflammatory drug. The preparation process is mild in condition, simple to operate and environment-friendly.
Owner:ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE

Salicornia polysaccharide, and preparation method and application thereof

The application discloses a Salicornia polysaccharide as well as a preparation method and application thereof. The Salicornia polysaccharide is composed of 11 kinds of monosaccharides, wherein arabinose (24.96%), galactose (30.39%) and galacturonic acid (23.20%) are main monosaccharides of the polysaccharide, and the relative molecular weight is 3.24*10 4 Da. The Salicornia polysaccharide has a very special surface morphological structure, and presents a regular sawtooth distribution. The anti-tumor activity of the Salicornia polysaccharide is researched, and it is found that the polysaccharide can inhibit the growth of HepG2 cells, and the molecular mechanism of the anti-tumor activity is that the polysaccharide can induce the apoptosis of HepG2 cells. The Salicornia polysaccharide has significant anti-tumor activity, can be used as a candidate drug for developing an anti-tumor drug, and has a wide development prospect.
Owner:YANCHENG INST OF TECH

Method for rapidly detecting monosaccharide composition in polysaccharide

The invention discloses a method for rapidly detecting monosaccharide composition in polysaccharide, and relates to the technical field of food analysis. The method comprises the following steps: firstly hydrolyzing polysaccharide by adopting TFA, then derivatizing PMP, and finally analyzing the monosaccharide composition of the derivatized polysaccharide hydrolysate by adopting UHPLC QQQ MS (Ultra High Performance Liquid Chromatography QQQ Mass Spectrometry). According to the method provided by the invention, the detection analysis of 18 monosaccharides can be quickly completed within 7 minutes, and the detection limit is also lower than that of a mainstream monosaccharide detection method HPAEC-PAD.
Owner:NANCHANG UNIV