74 results about "Monosaccharide composition" patented technology

The invention relates to the field of medicines, foods or health-care products, in particular to big-fruit hawthorn heteropolysaccharide as well as a preparation method and application thereof. The large-fruit hawthorn heteropolysaccharide is prepared from galacturonic acid, glucuronic acid, mannose, xylose, arabinose, glucose, galactose, fucose and rhamnose. The weight-average molecular weight of the big fruit hawthorn heteropolysaccharide is 81.6 kDa to 157.3 kDa. The product has unique structures of complex monosaccharide composition, low molecular weight, appropriate uronic acid content, low esterification degree and the like; compared with CP which is single in structure and high in esterification, the CP is better in oxidation resistance and bile acid binding capacity; compared with hawthorn polysaccharide with low uronic acid content and high molecular weight, the hawthorn polysaccharide has the advantages that the enzyme inhibition activity is obviously enhanced, and the hawthorn polysaccharide has a wide prospect in development of functional food, health care products or medicines for regulating blood sugar / blood fat, resisting oxidation and preventing metabolic syndromes.

The invention discloses a preparation method and application of roasted barley and malt polysaccharide with an anti-cancer effect. The roasted barley malt polysaccharide is a neutral heteropolysaccharide which mainly takes-> 3)-beta-D-Glcp-(1-> and->-beta-D-Glcp-(1->) as main chains, branches exist at O-3 / O-4 positions, and the branch chains are mainly composed of-> 4)-beta-D-Xylp-(1->,-> 3, 4)-beta-D-Xylp-(1-> and alpha-D-Glcp-(1->. The monosaccharide composition comprises arabinose, glucose and xylose in a molar mass ratio of 1.86: 33.43: 1.00, and the molecular weight is 23.81 kDa. In-vitro activity experiments prove that the roasted barley malt polysaccharide disclosed by the invention has a remarkable inhibition effect on proliferation of liver cancer cells, lung cancer cells, breast cancer cells and colorectal cancer cells, particularly on the liver cancer cells, and shows outstanding anti-tumor activity, so that the roasted barley malt polysaccharide is expected to be developed and applied in the fields of foods, health-care products or medicines.

The invention relates to the field of biological extraction, substance analysis, application and effect evaluation, in particular to an extraction method of polysaccharide of an overground part of chicory, the polysaccharide and a related application and evaluation method. The method comprises the following steps: providing an extraction method of the polysaccharide of the overground part of the chicory, carrying out reflux extraction, concentration, precipitation, centrifugation, washing and freeze-drying to obtain the polysaccharide, and carrying out molecular weight distribution and monosaccharide composition analysis on the polysaccharide, and the polysaccharide has antioxidant and anti-inflammatory activity, can be used for preparing products for protecting myocardial cells and the like, and has wide application prospects. The invention also discloses a composition containing the polysaccharide and an evaluation method for comparing the myocardial protection activity of the polysaccharide at different parts of the cichorium intybus. The application achieves the technical effects of efficiently extracting the polysaccharide of the overground part of the chicory, determining the characteristics and effects of the polysaccharide, providing a basis for development of products related to cardiovascular disease prevention and treatment, and providing a method for evaluating the myocardial protection activity of the polysaccharide of different parts of the chicory.

This invention discloses an arabinogalactan sulfate derived from *Streptococcus filamentosa*, its preparation method, and its applications, belonging to the fields of natural product chemistry and marine biological resource development technology. The technical solution includes an arabinogalactan sulfate derived from *Streptococcus filamentosa*, with a monosaccharide composition comprising arabinose, galactose, and rhamnose, the molar percentage of arabinose being not less than 70%; the main chain backbone is modified with natural sulfate groups, with the sulfate groups replacing at least one hydroxyl site of the arabinose residues. This invention, when applied to anticoagulation, exhibits good safety and a significant anticoagulant effect.

This invention provides a Shoumei polysaccharide, its preparation method, and its application, belonging to the field of biomedical technology. The Shoumei polysaccharide is a homogeneous polysaccharide with the repeating sequence →4)-α-D-Gal. p A‑(1→, →4)‑β‑D‑Gal p ‑(1→,→4,6)‑β‑D‑Gal p ‑(1→,→6)‑β‑D‑Gal p -(1→、α-L-Ara f ‑(1→,→5)‑α‑L‑Ara f ‑(1→,→4)‑β‑D‑Glc p -(1→; The molecular weight of the Shoumei polysaccharide is 1100 kDa; The monosaccharide composition of the Shoumei polysaccharide is arabinose, galactose, glucose and galacturonic acid; The molar ratio of arabinose, galactose, glucose and galacturonic acid is 10.9:12.1:4.8:72.2, and the degree of galacturonic acid esterification is 33%. The Shoumei polysaccharide of the present invention can treat and / or prevent depression.

This invention discloses the application of Uncaria rhynchophylla neutral polysaccharide URP-W in the preparation of anti-glioma drugs, belonging to the field of anti-tumor technology of medicinal plant polysaccharides. The invention obtains a crude polysaccharide extract from Uncaria rhynchophylla through hot water extraction and ethanol precipitation, followed by purification using a DEAE cellulose column and G200 dextran gel to obtain Uncaria rhynchophylla neutral polysaccharide URP-W. The monosaccharide composition of URP-W includes arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid, and mannuronic acid in a molar ratio of 17.99:6.32:21.71:27.17:2.56:7.57:16.20:0.49. Experiments have shown that URP-W can inhibit the proliferation of gliomas and induce apoptosis, exhibiting significant anti-tumor activity against glioma cells, providing a new drug raw material and treatment method for the clinical treatment of gliomas.

The invention discloses armillariella mycelium polysaccharide ATMP capable of improving cognitive impairment and a preparation method and application of the armillariella mycelium polysaccharide ATMP. The polysaccharide is composed of two components, and the molecular weights of the two components are 2.30 * 10 < 4 > Da and 4.56 * 10 < 5 > Da respectively; the monosaccharide composition comprises mannose (Man), glucose (Glc) and galactose (Gal), and the molar ratio of the mannose (Man) to the glucose (Glc) to the galactose (Gal) is 1: 16.64: 1.96. Animal experiment results show that the armillariella mycelium polysaccharide ATMP can improve cognitive impairment induced by lipopolysaccharide (LPS) by improving the spatial memory ability of mice, repairing the disorder state and deletion injury of neurons in a hippocampal region and relieving neuroinflammatory response, so that the obvious neuroprotective effect is achieved. As a natural brain-benefiting active component, the armillariella tabescens mycelium polysaccharide ATMP has a wide application prospect in the related fields of brain health.

The invention discloses a nuclear magnetic resonance hydrogen spectrum method for directly analyzing a high molecular weight hyaluronic acid structure and quantitative monomer composition. The method comprises the following steps: S1, dissolving a high-molecular-weight hyaluronic acid sample in D2O, adding NaOD, and uniformly mixing to obtain a sample solution; the concentration of the hyaluronic acid sample in the D2O is 0.5 to 2 mg / mL; the final concentration of the NaOD in the sample solution is 75 mmol / L; s2, performing a nuclear magnetic resonance hydrogen spectrum test on the sample solution to obtain a nuclear magnetic resonance hydrogen spectrum spectrogram; and S3, obtaining an integral area proportion of a hydrogen atom characteristic peak from the nuclear magnetic resonance hydrogen spectrum so as to directly quantify the composition molar ratio of the two monomers of the high-molecular-weight hyaluronic acid. The method provided by the invention not only can realize direct structural characterization of the high molecular weight HA, but also can realize direct quantification of the composition proportion of the two monosaccharides, and the quantitative result is high in precision and reproducibility.

The application discloses Ixeris sonchifolia Hance polysaccharides, a preparation method and application thereof, belongs to the technical field of plant polysaccharides, and provides polysaccharides ISP-1a and ISP-2a, wherein the ISP-1a has a molecular weight of 2086 Da and is composed of seven monosaccharides of mannose, rhamnose, glucuronic acid, glucose, galactose, xylose and arabinose; the ISP-2a has a molecular weight of 1781 Da and is composed of five monosaccharides of mannose, rhamnose, galacturonic acid, xylose and arabinose. The whole grass of Ixeris sonchifolia Hance is subjected to hot water extraction after ethanol degreasing, neutral enzyme + Sevage method is used to remove protein, macroporous resin is used for decolorization, column chromatography elution is carried out, purification is carried out, and two kinds of uniform polysaccharides are obtained after freeze-drying. The polysaccharides ISP-1a and ISP-2a have good antioxidant and lipid-lowering effects, and can be applied to the preparation of food, health products or medicines with antioxidant and lipid-lowering functions.

The application discloses radix sophorae tonkinensis polysaccharide STGP-1, a preparation method thereof and pharmaceutical use for treating lung squamous carcinoma, and relates to the technical field of medicines. The monosaccharide composition of STGP-1 includes fucose Fuc, arabinose Ara, galactose Gal, glucose Glc, xylose Xyl and mannose Man, and the molar ratio is 0.34:12.15:25.76:57.07:1.06:3.62. The STGP-1 is obtained by extraction, separation and purification from dried roots and rhizomes of radix sophorae tonkinensis, and the preparation method comprises the steps of pretreatment and crude extraction, impurity removal treatment, anion exchange column chromatography separation, gel column chromatography purification and the like. The STGP-1 can be used alone or in combination with anti-PD-1 monoclonal antibody for preparing a medicine for treating lung squamous carcinoma. The preparation process is stable, the source is natural, and the STGP-1 can provide a new high-efficiency and low-toxicity candidate medicine for immune combined treatment of lung squamous carcinoma.

The invention belongs to the technical field of plant polysaccharide, and particularly discloses caulis spatholobi polysaccharide, a preparation method thereof and application of the caulis spatholobi polysaccharide in preparation of medicines for treating inflammation. Monosaccharide in the caulis spatholobi polysaccharide disclosed by the invention comprises mannose, glucuronic acid, galacturonic acid, glucose and arabinose in a molar ratio of (0.5-0.6): (0.3-0.5): (1-2): (1-2): (0.5-1.5). The caulis spatholobi polysaccharide provided by the invention is a novel uniform-structure polysaccharide which is separated from caulis spatholobi rattan for the first time, has a slightly acidic pH value, definite monosaccharide composition, definite molecular weight and clear glycosidic bond linking mode, has an anti-inflammatory effect, and is expected to be developed into a new anti-inflammatory drug. The preparation process is mild in condition, simple to operate and environment-friendly.

The application discloses a Salicornia polysaccharide as well as a preparation method and application thereof. The Salicornia polysaccharide is composed of 11 kinds of monosaccharides, wherein arabinose (24.96%), galactose (30.39%) and galacturonic acid (23.20%) are main monosaccharides of the polysaccharide, and the relative molecular weight is 3.24*10 4 Da. The Salicornia polysaccharide has a very special surface morphological structure, and presents a regular sawtooth distribution. The anti-tumor activity of the Salicornia polysaccharide is researched, and it is found that the polysaccharide can inhibit the growth of HepG2 cells, and the molecular mechanism of the anti-tumor activity is that the polysaccharide can induce the apoptosis of HepG2 cells. The Salicornia polysaccharide has significant anti-tumor activity, can be used as a candidate drug for developing an anti-tumor drug, and has a wide development prospect.

The invention relates to lentinan as well as a preparation method and application thereof, monosaccharide of the lentinan comprises glucose, galactose, mannose, fucose and glucosamine, and the molar ratio of the glucose to the galactose to the mannose to the fucose to the glucosamine is (85.0 to 88.0) to (5.5 to 6.5) to (3.5 to 4.1) to (1.8 to 2.2) to (1.0 to 1.2). According to the preparation method disclosed by the invention, a composite filler consisting of AB-8 macroporous resin and acidic aluminum oxide is adopted, static adsorption decoloration is carried out when the optimal mass ratio is 2: 3, an extraction process of combining graded hot water extraction with negative pressure concentration is optimized, and on the premise of avoiding using an organic solvent, efficient purification of lentinan is realized, so that the retention rate of the lentinan reaches 22.38%, and the yield of the lentinan is improved. And the beta-triple helix active structure can be well reserved.

The present application provides a kind of Pseudostellaria heterophylla non-starch uniform polysaccharide and its preparation method and application, belong to natural substance extraction technical field.The Pseudostellaria heterophylla non-starch uniform polysaccharide is divided into five kinds, molecular weight is 2124Da, 85900Da, 72970Da, 35820Da and 20300Da respectively, all have three spiral structures.The Pseudostellaria heterophylla non-starch uniform polysaccharide with molecular weight of 2124Da includes the following molar ratio of glucose, galactose and mannose: 95.47:2.15:2.37, and its repeatable primary structure unit includes the following sugar residues: T-Glcp, 1,3-Glcp, 1,2-Galp, 1,3,6-Manp, 1,4,6-Glcp.The Pseudostellaria heterophylla non-starch uniform polysaccharide of the present application has excellent prebiotic activity and simple preparation method, and the obtained Pseudostellaria heterophylla non-starch uniform polysaccharide has stable yield, high purity and stable structure, and is suitable for industrial scale production.

The invention provides high-activity polygonatum polysaccharide as well as a preparation method and application thereof, and belongs to the technical field of effective component extraction. A mixture of cellulase, hemicellulase and pectinase is used as a compound enzyme preparation for enzymolysis, extraction and purification to obtain the polygonatum polysaccharide PCPA. The molecular weight of the polygahatous polysaccharide PCPA is 1372 Da, the polygahatous polysaccharide PCPA is composed of three monosaccharides of arabinose, glucose and fructose, the molar ratio of the three monosaccharides is 0.39: 6.48: 93.13, and the polygahatous polysaccharide PCPA has the effects of delaying cell senescence and improving cell repair activity.

The application discloses a chaga acidic polysaccharide IOP-2 with anti-thyroid cancer activity and application thereof, and belongs to the field of biological medicines. The application adopts a method for extracting chaga polysaccharide by boiling water and precipitating chaga polysaccharide by ethanol, and combines DEAE cellulose column purification technology, and successfully realizes separation of chaga polysaccharide by using the principle that macromolecular polysaccharide is insoluble in organic solvents, and obtains chaga acidic polysaccharide IOP-2 with anti-thyroid cancer activity. The monosaccharide composition of the chaga acidic polysaccharide IOP-2 includes fucose 0.71%, rhamnose 3.33%, arabinose 2.18%, galactose 2.92%, glucose 80.59%, xylan 3.46% and mannose 6.81% in terms of molar percentage. The significant effect of the chaga acidic polysaccharide IOP-2 on anti-thyroid cancer is verified through in-vivo and in-vitro experiments, and a new treatment method is provided for clinical treatment of thyroid cancer.

The invention discloses cordyceps sobolifera polysaccharide with an effect of reducing blood pressure and a microbial fermentation preparation method of the cordyceps sobolifera polysaccharide, and belongs to the field of preparation and application of fungal polysaccharide extracts. The cordyceps sobolifera polysaccharide is prepared by adopting a microbial fermentation method, cordyceps sobolifera coremium is used as a fermentation substrate, plant lactobacillus is used as a fermentation strain, liquid fermentation extraction is performed to obtain the cordyceps sobolifera polysaccharide CCP-Lp, the monosaccharide composition and the molar ratio of mannose to glucose to galactose are 1: 0.36: 0.62, and the relative molecular mass is 1.32 * 10 < 5 > Da. Efficacy experiments show that CCP-Lp can significantly inhibit abnormal increase of systolic pressure and diastolic pressure of spontaneously hypertensive rats and regulate the key factor level of a renin-angiotensin-aldosterone system, and the antihypertensive effect of CCP-Lp can be comparable with that of a positive drug captopril. The cordyceps sobolifera polysaccharide CCP-Lp is clear in composition and remarkable in effect, and has wide development and application prospects in the aspect of blood pressure regulation and control.

The application discloses a cordyceps cicadae and cordyceps sinensis polysaccharide with blood pressure lowering efficacy and a microbial fermentation preparation method thereof, and belongs to the field of fungal polysaccharide extract preparation and application. The cordyceps cicadae and cordyceps sinensis polysaccharide is prepared by a microbial fermentation method, a cordyceps cicadae and cordyceps sinensis spore bundle is used as a fermentation matrix, and bacillus lactis is used as a fermentation strain; after liquid fermentation extraction, the cordyceps cicadae and cordyceps sinensis polysaccharide CCP-Lp is obtained, the monosaccharide composition and molar ratio of which are as follows: mannose: glucose: galactose = 1: 0.36: 0.62, and the relative molecular mass is 1.32*10 5 Da. Efficacy experiments show that the CCP-Lp can significantly inhibit abnormal increase of systolic pressure and diastolic pressure of spontaneous hypertension rats, and regulate levels of key factors of a renin-angiotensin-aldosterone system; the antihypertensive effect of the CCP-Lp is comparable to that of a positive drug, captopril. The cordyceps cicadae and cordyceps sinensis polysaccharide CCP-Lp has clear composition and significant efficacy, and has a broad development and application prospect in regulating blood pressure.

The invention discloses porphyra yezoensis polysaccharide PSP-I with anti-ultraviolet damage activity as well as a preparation method and application of the porphyra yezoensis polysaccharide PSP-I. The Mw of the PSP-I is 26.149 kDa, the Mn of the PSP-I is 11.267 kDa, and the molar ratio of galactose to glucose to glucuronic acid in monosaccharide composition of the PSP-I is 96.76: 2.31: 0.93. The preparation method comprises the following steps: pretreating a nori raw material, extracting crude polysaccharide by adopting an enzyme method, deproteinizing, dialyzing, collecting a main polysaccharide elution peak by utilizing anion exchange chromatography, further purifying by virtue of sephadex chromatography, collecting the main elution peak, removing impurities and drying, thereby obtaining the nori polysaccharide. The polysaccharide PSP-I can effectively remove active oxygen induced by UVB, inhibit apoptosis and down-regulate expression of matrix metalloproteinase in cells under a safe concentration, shows excellent anti-light aging activity, and can be used for preparing cosmetics or skin care products for resisting ultraviolet damage.

The invention discloses a stringy stonecrop herb polysaccharide extraction process which comprises the following steps: drying and crushing stringy stonecrop herb, adding water according to a material-liquid ratio of (1: 20)-(1: 60), extracting, concentrating, and carrying out alcohol precipitation to obtain crude polysaccharide; deproteinizing, dialyzing and freeze-drying; carrying out gradient elution and purification to obtain sedum sarmentosum polysaccharide SSBP; an extraction process is optimized through a response surface method, optimal conditions are selected, and the yield of crude polysaccharide reaches 19.06%; according to structural characterization, SSBP is acidic polysaccharide, the molecular weight is 7.484 kDa, 36.12% of uronic acid is contained, monosaccharide is composed of rhamnose, arabinose, galactose, galacturonic acid and glucuronic acid, and the molar ratio of rhamnose to arabinose to galactose to galacturonic acid to glucuronic acid is 12.85: 19.13: 18.98: 35.73: 3.35; an in-vitro activity experiment shows that the SSBP has good antioxidant activity and remarkable anti-inflammatory activity, the release of RAW264.7 macrophage NO induced by LPS and the secretion of IL-6, IL-1beta and TNF-alpha can be inhibited in a dose-dependent manner, and the inhibition rates under the concentration of 1.2 mg / mL respectively reach 74.41%, 92.53%, 95.27% and 95.73%; and a new scheme is provided for deep development of sedum sarmentosum polysaccharide and preparation of anti-inflammatory and anti-oxidation products.

The application discloses a rehmannia polysaccharide, a preparation method thereof and application of the rehmannia polysaccharide in preparation of a medicine for preventing and treating kidney injury caused by cisplatin. The rehmannia polysaccharide contains the following monosaccharides in percentage by mass: rhamnose (Rha) 10.265%-14.963%, glucuronic acid (GlcA) 0.514%-0.904%, galacturonic acid (GalA) 35.784%-40.596%, glucose (Glc) 3.128%-5.048%, galactose (Gal) 37.892%-41.438% and arabinose (Ara) 3.869%-4.865%. The structure of the rehmannia polysaccharide is clear, and the rehmannia polysaccharide shows a significant improvement effect on cisplatin nephrotoxicity through cell experiments and animal experiments, and has important value for developing a new and effective prevention and treatment product of cisplatin-induced acute kidney injury.

The invention relates to the technical field of natural product extraction and purification and biological medicine, in particular to day lily polysaccharide with broad-spectrum antibacterial activity and application of the day lily polysaccharide. The molecular weight of the hemerocallis citrina polysaccharide is 1959 Da, monosaccharide components of the hemerocallis citrina polysaccharide mainly comprise fructose (88.87%) and glucose (11.13%), and the hemerocallis citrina polysaccharide is high-fructose neutral polysaccharide. The main chain is-> 1)-beta-D-Fruf-(2->), side chains are connected to O-6 sites of partial main chain residues, and the side chains comprise beta-D-Fruf-(2->) and-> 6)-beta-D-Fruf-(2->); the molar percentages of the sugar residues Fruf1, 2, Fruf2, Fruf1, 2, 6, Fruf2, 6 and Glc1, 6 are respectively 41.94%, 23.59%, 9.27%, 16.80% and 8.40%, and the molar percentages of the sugar residues Fruf1, 2, Fruf2, Fruf2, Fruf6 and Glc1, 6 are respectively 41.94%, 23.59%, 9.27%, 16.80% and 8.40%; the hemerocallis citrina baroni polysaccharide shows remarkable bacteriostatic activity on clinically separated multi-drug-resistant strains and standard strains, and shows dose dependence; in addition, the improvement effect of the hemerocallis citrina polysaccharide on the lung tissue injury is enhanced along with the increase of the dosage, the effect can be quantified and repeatable, and the requirements of drug development are met.

The present application belongs to the technical field of natural polymer materials, and particularly relates to a purified polysaccharide of Rhizoma Polygonati, a preparation method thereof and application of the purified polysaccharide in preparation of a medicine for relieving mammary gland hyperplasia. The purified polysaccharide of Rhizoma Polygonati comprises xylose, mannose, glucose and galactose, and the molar ratio of the xylose, mannose, glucose and galactose is 6.45:30.26:7.43:55.87. The molecular weight distribution of the purified polysaccharide of Rhizoma Polygonati is within 2000-30000 Da, and the molecular weight is concentrated at 22877 Da. The purified polysaccharide of Rhizoma Polygonati can reduce the toxic damage of estrogen and progesterone imbalance to HC11 mammary epithelial cells, and a low concentration of the purified polysaccharide of Rhizoma Polygonati can produce a protective effect on the cells. The purified polysaccharide of Rhizoma Polygonati is verified to have a significant inhibitory effect on mammary gland hyperplasia of mice through a mammary gland hyperplasia model intervention experiment, and therefore can be used for developing a medicine for treating mammary gland hyperplasia.

The invention belongs to the technical field of marine polysaccharides, and relates to an anti-hypoxia urechis unicinctus body cavity fluid sulfated polysaccharide as well as a preparation method and application thereof. The weight-average molecular weight of the polysaccharide is 1000-50000 Da, the mass fraction of sulfate radicals is 10.0%-30.0%, and monosaccharide is prepared from galactose, ribose, fucose, glucosamine, mannose and glucose. The preparation method comprises the following steps: inducing controlled degradation of a main chain through high-temperature hot water extraction, carrying out proteolysis deproteinization, carrying out graded ethanol precipitation interception and carrying out anion exchange chromatography purification. The polysaccharide shows a remarkable protective effect in a nerve cell hypoxia simulation model, has excellent free radical scavenging activity, and can be used for preparing medicines, antioxidants, functional foods and the like for preventing or treating hypoxia-related diseases.

The invention discloses an application of fried highland barley malt polysaccharide in preparation of an anti-tumor preparation. The highland barley roasted malt polysaccharide is heteropolysaccharide of which monosaccharide composition comprises arabinose, glucose and xylose in a molar mass ratio of 1.00: 3.75: 1.54. Research finds that the barley fried malt polysaccharide can significantly inhibit proliferation of liver cancer cells, lung cancer cells, breast cancer cells and colorectal cancer cells, especially has significant anti-tumor activity on the colorectal cancer cells, and is expected to be applied to food, health care products or drugs.

The invention discloses lithocarpus litseifolius polysaccharide LLP-2 as well as a preparation method and application thereof, and relates to the technical field of natural product extraction and medical application. According to the extraction and purification method provided by the invention, the high-purity lithocarpus litseifolius polysaccharide can be effectively obtained, the polysaccharide is an acidic heteropolysaccharide composed of a plurality of monosaccharides, the structural characteristics and morphology of the polysaccharide are preliminarily characterized by a modern analysis technology, and the method is stable in process, good in repeatability and suitable for industrial production. And a material basis and a research basis are provided for deep development and functional application of the lithocarpus litseifolius polysaccharide.

The invention discloses a preparation method and application of aronia melanocarpa fruit homogeneous polysaccharide freeze-dried powder, and belongs to the field of biotechnology and functional food. By optimizing extraction, separation and purification processes, a homogeneous polysaccharide freeze-dried component is obtained from aronia melanocarpa fruits for the first time; the molecular mass, monosaccharide composition, glucosidic bond type and purity of the polysaccharide component are defined; in addition, it is further found from multiple aspects that the component has a good immunity enhancing effect and has important application potential in functional food and health care products for enhancing immunity.

The invention discloses a wolfberry-astragalus compound polysaccharide with an anti-myelosuppression effect as well as a preparation method and application of the wolfberry-astragalus compound polysaccharide. The wolfberry-astragalus compound polysaccharide is prepared by adopting a process of separately extracting wolfberry polysaccharide and astragalus polysaccharide and then mixing, the total sugar content is 70-80%, the uronic acid content is 10-14%, and the wolfberry-astragalus compound polysaccharide has specific molecular weight distribution and monosaccharide composition characteristics. Animal experiment results show that the wolfberry and astragalus compound polysaccharide can significantly improve peripheral hemopenia of myelosuppression mice induced by cyclophosphamide, promote recovery of the number of leukocytes, lymphocytes, monocytes and neutrophils, and effectively reverse weight loss caused by chemotherapeutic drugs. The wolfberry-astragalus compound polysaccharide provided by the invention is high in safety and remarkable in anti-myelosuppression effect, and can be used for preparing medicines or functional preparations for improving myelosuppression related diseases.

The invention belongs to the technical field of biological medicine, and relates to sargassum polysaccharide as well as a preparation method and application thereof. The uniform acidic polysaccharide WZSP-2 is separated and purified from the Weizhou Sargassum through hot water extraction, ethanol precipitation, protein and color removal, anion exchange column and gel column chromatography and other methods, and the physicochemical properties, monosaccharide composition and structural morphology of the Weizhou Sargassum are systematically characterized. In-vitro experiments show that the WZSP-2 has remarkable DPPH, ABTS and hydroxyl radical scavenging capacity and total reducing power, can effectively inhibit the activity of alpha-amylase and alpha-glucosidase, can be combined with glycocholate and taurocholate, and has a bacteriostatic effect on methicillin-resistant staphylococcus aureus (MRSA) at the same time. The sargassum polysaccharide provided by the invention integrates antioxidant activity, hypoglycemic activity, hypolipidemic activity and antibacterial activity, provides a new source for developing multifunctional natural products, and has a good application prospect in the fields of functional food, health care products and medical auxiliary materials.