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160 results about "Anoectochilus" patented technology

Anoectochilus, commonly known as marbled jewel orchids or filigree orchids, is a genus of about fifty species in the orchid family Orchidaceae. They are terrestrial herbs with a creeping rhizome, an upright flowering stem and dark coloured leaves with contrasting veins. The flowers are relatively large and have a large labellum, markedly different from the sepals and petals.

Undergrowth cultivation medium and cultivation method for anoectochilus formosanus

The invention relates to an undergrowth cultivation medium and cultivation method for anoectochilus formosanus. The undergrowth cultivation method for the anoectochilus formosanus comprises the steps of (1) selecting a forest environment with a natural shading degree of 60-90% and illumination intensity of 2000-5000lux to serve as a cultivation place of the anoectochilus formosanus; (2) forming shallow holes in the cultivation substrate in a scratched mode, inserting the root of each anoectochilus formosanus seedling into each shallow hole, covering each root, and watering rooting water; (3) adjusting air humidity to be 75-90%, adjusting the temperature to be 15-30DEG C; (4) forming the cultivation medium, wherein the cultivation medium mainly comprises 8-23 parts of wood chip smashed objects, 8-25 parts of differentiated yellow loam and 65-80 parts of under-forest humus soil in a mixing mode; and (5) carrying out spraying watering when the surface of the cultivation medium whites after planting, and stopping watering when the cultivation medium is thoroughly watered every time. According to the undergrowth cultivation medium and cultivation method for the anoectochilus formosanus, due to the facts that the forest environment which is in natural growth and has the appropriate shading effect serves as the cultivation base, and the high-quality and high-efficiency cultivation medium is selected, growth of the anoectochilus formosanus is facilitated, the yield, the survival rate and the drying rate of the anoectochilus formosanus can be remarkably improved, operation is convenient, and production cost is low.
Owner:福建省农业科学院农业生物资源研究所

Greenhouse cultivation method for anoectochilus formosanus

The invention discloses a greenhouse cultivation method for anoectochilus formosanus. The greenhouse cultivation method comprises the four steps of acclimating anoectochilus formosanus seedlings, disinfecting a cultivation substrate, planting, and controlling temperature and light, wherein in the step of planting, a cultivation cup after the anoectochilus formosanus seedlings are planted is placed into a plastic bag, and a plurality of small holes are formed in the position where the plastic bag higher than the cultivation cup; and water is poured into the bottom of the plastic bag, a bag opening in the plastic bag is sealed, and the plastic bag is hung below a seedling bed in a greenhouse. Due to the adoption of the technical scheme, the water is evaporated in the bag, so that the water is not liable to run away and a wetting environment suitable for growth of the anoectochilus formosanus is formed in the plastic bag. The anoectochilus formosanus seedlings are spaced from the outside environment through the plastic bag, the water is not liable to run away and the anoectochilus formosanus seedlings do not need to be watered in the later period after being planted, so that the anoectochilus formosanus seedlings cannot be polluted by various bacteria in air or water and the pest and disease damage is avoided. Therefore, higher survival rate of the anoectochilus formosanus seedlings can be also guaranteed in the absence of farm chemicals.
Owner:郑泽新

Key technology of organic additive for roxburgh anoectochilus terminal bud industrialization intermediate propagation

The invention provides a key technology of an organic additive for roxburgh anoectochilus terminal bud industrialization intermediate propagation, belonging to a massive propagation and cultivation technology for the roxburgh anoectochilus terminal bud. According to the key technology, a terminal bud and a stem of the roxburgh anoectochilus terminal bud are taken as the material, in industrialization, the organic additive is adjusted and added timely, and the entire plant grows up after induction cultivation, enrichment cultivation and root induction, and the rooting percentage can reach 99%; and after hardening-seedling, the plant can be transplanted into humus, and the rate of survival can reach above 99%. According to the technology, the time for enrichment cultivation is short, the expanding propagation coefficient is high, the seedling can grow up once and is healthy, the whole roxburgh anoectochilus terminal bud plant is thick, the stem is thick and the roots are flourishing, the color of leaves is dark, the leaves have obvious fine hair, the leaf vein gold threads are clear, the rate of survival is high, the weight of each plant is 4.5-5.5g, and the economic efficiency is obvious.
Owner:福建海默农业科技发展有限公司

Interplanting cultivation device for anoectochilus formosanus and dendrobium officinale and method thereof

The invention provides an interplanting cultivation device for anoectochilus formosanus and dendrobium officinale. The interplanting cultivation device comprises a thin film greenhouse and an interplanting shelf placed in the thin film greenhouse. The interplanting shelf is a three-dimensional triangular shelf formed by two longitudinal isosceles triangle frames and three transverse parallel rectangular frames. Dendrobium officinale planting frames formed by mutually stacking trunks and/or bamboo rods are placed on an upper layer and a middle layer. An anoectochilus formosanus planting tray shelf is arranged on a lower layer. The dendrobium officinale is planted into gaps among the upper and lower trunks of the upper layer and the middle layer of the interplanting shelf. The anoectochilus formosanus is planted in planting trays, with matrixes, on the lower layer of the shelf. The relative humidity in the thin film greenhouse is controlled to be 70-90%, the temperature is controlled to be 18-25 DEG C, the light transmittance is 60-80%, and spraying and ventilating facilities are arranged. Roots of the dendrobium officinale adhere to the trunks so as to shade the anoectochilus formosanus on the lower layer, the anoectochilus formosanus on the lower layer is cultivated in a lifting mode by adopting the matrixes, the water permeability is good, soil insect harm can be avoided, organic fertilizer sprayed from the upper layer can be fully utilized, the planting cost is lower, the harvesting reasons are spaced, and benefits are higher.
Owner:NINGBO ACAD OF AGRI SCI

Medicinal anoectochilus formosan stem-cutting root-retaining regeneration planting method

InactiveCN101233808AGuaranteed healthy growthPromote germination and regenerationClimate change adaptationHarvestersDiseaseAnoectochilus
The invention discloses a method for regenerating and planting medicinal anoectochilus roxburghii with the stem removed but the root left, which has the key point of regeneration with the root left and stem removed. First, strong tissue culture seedling are elected and then transmitted to be planted in a mixed substrate consisting of soil, coarse sand and wood dust after plantlet hardening and watered with a mixed liquid of root agent and carbendazim wettable powder. Field management, fertilization and disease preventing and treating are required to be respected during the growth period of the anoectochilus roxburghii. By applying the method of regeneration with the stem removed and the stem left, the anoectochilus roxburghii stems on the ground are harvested but a stem node at each stem is left before the bolting and blossom of the anoectochilus roxburghii. After harvesting, the remained plant is sprayed with a mixed liquid of 6-benzyl aminopurine and carbendazim wettable powder. The method aims at solving the problem of narrow availability of the anoectochilus roxburghii seedling and easily sprouting of the stem node thereof and adopts the production method of regeneration with the stem removed and the stem left, thereby improving the seedling use ratio, guaranteeing the product supply and realizing continuous production. Meanwhile, the complete retaining of the root system creates the good condition for the quick growth of new sprouts and therefore, the regeneration method of the invention has comparatively high production efficiency and good economic benefit.
Owner:浙江省中药研究所有限公司

Hormone-free rapid tissue culture and propagation method of Emei roxburgh anoectochilus terminal bud seeds

The invention discloses a hormone-free rapid tissue culture and propagation method of Emei roxburgh anoectochilus terminal bud seeds. According to the hormone-free rapid tissue culture and propagation method, no hormone is used in the whole process, the mature and non-cracking fruits, which is pollinated for 5 months after flowering, of wild or artificially planted roxburgh anoectochilus terminal bud distributed in Szechuan Emei region are taken as the material, and seedlings are bred efficiently and quickly according to the route from seeds to protocorms, complete plants and acclimatization and transplant. A hormone-free culture medium is adopted in the whole process; more than 5000 good-quality seedlings can be quickly bred from one mature fruit by virtue of tissue culture of about 10-12 months, and after acclimatization and transplant, the greenhouse culture survival rate can be above 95%. According to The hormone-free rapid tissue culture and propagation method of the Emei roxburgh anoectochilus terminal bud seeds is capable of keeping the good traits of the Emei roxburgh anoectochilus terminal bud and maintaining the good medicinal properties of the Emei roxburgh anoectochilus terminal bud; besides, the method is simple, and the cost is low; industrialized seedling production can be realized; no hormone is adopted so that the safety of the seedlings can be ensured; the hormone-free rapid tissue culture and propagation method has important significance on regeneration and sustainable utilization of critically endangered valuable and rare resources of medicinal plants and protection of seed resources.
Owner:四川省自然资源科学研究院 +1

Tissue culture rapid propagation method of Emei anoectochilus formosanus

InactiveCN103688854ARealize commercial scale cultivationGood characterPlant tissue cultureHorticulture methodsGrowth coefficientGreenhouse
The invention discloses a tissue culture rapid propagation method of anoectochilus formosanus, and belongs to the technical field of plant tissue culture. By adopting the tissue culture rapid propagation method, the survival rate of transplanting and the growth coefficient can be increased, and the good characters and the good drug properties of the Emei anoectochilus formosanus can be kept. The tissue culture rapid propagation method comprises the following steps: selecting wild Emei anoectochilus formosanus growing in May to October, preparing an explant, carrying out disinfection treatment, inducing cluster buds, carrying out secondary culture and rooting and strengthening culture, and transplanting to greenhouses for culturing, and rapidly propagating the anoectochilus formosanus through hormone induction. The tissue culture rapid propagation method can be used for ensuring the good characters and the good drug properties of the Emei anoectochilus formosanus, is simple and low in cost and can realize industrialized seedling production. The percentage of inducing the cluster buds and rooting by adopting the method reaches above 90%, the survival rate of transplanting is above 95%, and the growth coefficients within four months can reach 8-10 times. The Emei anoectochilus formosanus produced by adopting the method does not mutate, is high in yield, low in cost and short in period and has relatively strong market competitiveness.
Owner:四川省自然资源科学研究院 +1

Preparation method of roxburgh anoectochilus terminal bud extracts containing kinsenoside and application of extracts

The invention provides a preparation method of a roxburgh anoectochilus terminal bud extracts containing kinsenoside and application of the extracts. The roxburgh anoectochilus terminal bud extracts are is prepared from roxburgh anoectochilus terminal bud extraction liquid, roxburgh anoectochilus terminal bud thick paste, roxburgh anoectochilus terminal bud dry paste and roxburgh anoectochilus terminal bud dry paste powder. The preparation method comprises the work procedures of medical material alcohol extraction, water extraction, reduced pressure concentration, drying, crushing and the like. The method has the advantages that the transfer rate of the effective ingredient of the kinsenoside can be improved by 50 percent or higher through being comparedin comparison with a conventional water extraction process; the utilization rate of the rare medicinal materials is obviously improved; the process operability is high; the repeated performance is good; the equipment requirement is low;the preparation method is applicable to industrialized production. The roxburgh anoectochilus terminal bud extracts prepared by the method can be used for preparing health care food; the taking and the carrying of the finished product are is convenient to take and carry; the quality is stable; the auxiliary protection effect is achieved on the chemical liver damage; the effects of assistance in reducing the blood fat in an auxiliary way and reducing the blood sugar in an auxiliary way areis obvious.
Owner:HUAZHONG UNIV OF SCI & TECH

Molecular marker fingerprinting for identifying anoectochilus formosanus produced at Huoshan in Anhui and similar species thereof

The invention discloses a molecular marker fingerprinting for identifying the anoectochilus formosanus produced at Huoshan in Anhui and the similar species thereof. The molecular marker fingerprinting comprises the steps of preprocessing anoectochilus formosanus samples, extracting DNA of each sample genome, executing ISSR-PCR (inter-simple sequence repeat-polymerase chain reaction), executing agarose gel electrophoresis, building ISSR molecular marker fingerprint of each sample and using the ISSR molecular marker fingerprint for identifying the anoectochilus formosanus germplasm. The method provided by the invention saves time and economic cost, and can obtain the accurate and reliable identification fingerprint just by extracting the DNA of the anoectochilus formosanus plants, executing ISSR-PCR and executing agarose gel electrophoresis; the method identifies the species, which are easy to confuse in appearance, from the essence of inheritance, has the advantages of being simple, convenient and efficient, excellent in repeatability and high in resolution, and so on; and the method can identify the species in seeding stage of plants, and plays an important role in guaranteeing the accuracy and the stability of the Huoshan anoectochilus formosanus crude drug base source.
Owner:安徽同济生生物科技有限公司

Underwood planting method of anoectochilus formosanus

The invention discloses an underwood planting method of anoectochilus formosanus. The method is characterized by including the steps that first, forest land is prepared, wherein, a plurality of water spraying pipes are arranged above a forest site, an underwood soil layer is ploughed so as to loosen and break the soil layer, and a dead leaf layer with the thickness ranging from 2 cm to 3 cm is laid above the ploughed soil layer; second, anoectochilus formosanus seedlings are planted, wherein the anoectochilus formosanus seedlings are obtained through tissue culture and planted into the ploughed soil layer; third, underwood management is performed, wherein in the growth stage of the anoectochilus formosanus seedlings, when the dead leaf layer is in a dried state, the water spraying pipes are adopted for spraying water, and therefore the dead leaf layer can be kept moist. According to the method, dead leaves are stimulated to decompose with a fermentation method to generate small molecule nutrient substances capable of being easily absorbed by anoectochilus formosanus, meanwhile, dead leave powder can provide a good moist environment for anoectochilus formosanus, and therefore the fresh weight of the anoectochilus formosanus and the content of medical composition of the anoectochilus formosanus are increased.
Owner:XIAMEN JINTAIGU AGRI TECH CO LTD

Method for cultivating anoectochilus formosanus in wild imitation mode

InactiveCN103960030AImprove the survival rate of artificial plantingImprove survival rateHorticultureAnoectochilusEarth surface
The invention relates to the field of plant cultivating, in particular to a method for cultivating anoectochilus formosanus in a wild imitation mode. The method comprises the following steps that (1) seedling exercising is carried out on anoectochilus formosanus tissue cultured seedlings in a pre-cultivating field, and the pre-cultivating field is an earth surface humus horizon under an original ecological broad leaved forest; (2) the excised anoectochilus formosanus tissue cultured seedlings are taken out of tissue culturing bottles, and culturing media are washed away through running water; (3) the tissue cultured seedlings washed in the step (2) are steeped with a carbendazim solution for ten minutes; (4) anoectochilus formosanus cultivating soil is disinfected before the anoectochilus formosanus is cultivated, root systems of the anoectochilus formosanus in the step (3) are straightened and buried in the disinfected soil, and root fixing water is applied; (5) cultivating management is carried out on the anoectochilus formosanus until harvesting is conducted. According to the method, the anoectochilus formosanus is cultivated in the earth surface humus horizon of the original ecological broad leaved forest, the earth surface humus horizon of the original ecological broad leaved forest originally has a large amount of distributed wild anoectochilus formosanus and is quite suitable for cultivating the anoectochilus formosanus in the wild imitation mode, and the survival rate of the anoectochilus formosanus cultivated artificially is improved.
Owner:FUJIAN PROVINCE SHAOWU CITY ERDU STATE OWNED FOREST FARM

Method for promoting germination and seedling formation of anoectochilus formosanus seeds

InactiveCN106856713ASolve the technical problem of low efficiency of natural germination and seedling formationPromote germinationFungiMicroorganism separationAnoectochilusEndophytic fungus
The invention provides a method for promoting germination and seedling formation of anoectochilus formosanus seeds. According to the technical scheme, an idea that the anoectochilus formosanus seeds can germinate only through symbiosis with fungi is found by virtue of an experimental measure, a set of fungus acquiring methods and symbiosis culture conditions are explored under the guidance of the idea, meanwhile, a strain capable of outstandingly promoting the germination and seedling formation of the anoectochilus formosanus seeds on a sawdust matrix is definitized. Particularly, the method comprises the steps of separating endophytic fungi from the root of wide anoectochilus formosanus, mixing a mixed fungal colony or a purified specific colony with the disinfected anoectochilus formosanus seeds in the sterile sawdust matrix, culturing for one week under a dark condition, and then culturing at specific illumination intensity and temperature for specific illumination time until the seeds germinate. According to the technical scheme, the germination velocity of the anoectochilus formosanus seeds is increased, meanwhile, the method has good repeatability due to a clear principle.
Owner:NANCHANG UNIV

Method for preparing anoectochilus formosanus vinegar through liquid fermentation

The invention discloses a method for preparing anoectochilus formosanus vinegar through liquid fermentation. The method comprises the following steps of (1) preparing a fermented vinegar solution by carrying out liquid fermentation on sugarcane processing residues serving as raw materials, namely cleaning the sugarcane processing residues, smashing, then, mixing the sugarcane processing residues and water, adding a compound enzyme into the mixture to carry out liquification fermentation, and then, adding a saccharifying enzyme to carry out saccharification fermentation; next, adding an active dry yeast to carry out alcohol fermentation; and finally, adding activated acetic bacteria to carry out acetic fermentation, filtering and collecting filtrate; (2) preparing dry powder of anoectochilus formosanus, namely cleaning annual anoectochilus formosanus plants, drying, and smashing for later use; and (3) preparing the anoectochilus formosanus vinegar, namely mixing the fermented vinegar solution prepared by carrying out liquid fermentation on the sugarcane processing residues serving as the raw materials and the dry powder of the anoectochilus formosanus, uniformly stirring the mixture, processing at high temperature, and airing at normal temperature to obtain the anoectochilus formosanus vinegar. The anoectochilus formosanus vinegar prepared by using the method is soft in sour, mellow in taste, faint scent and elegant and is also rich in the anoectochilus formosanus and beneficial components in sugarcane bagasse and leaves. The method is easy to master and capable of realizing large-scale production, waste utilization and environment protection.
Owner:WEST ANHUI UNIV

Formula for culturing tissues with buds and stems of roxburgh anoectochilus terminal bud to seedlings by one step

The invention provides a formula for culturing tissues with buds and stems of roxburgh anoectochilus terminal bud to seedlings for one step. Each liter of a nutrient solution comprises the following components: 1-3g of hyponex I, 1-2g of hyponex II, 0.5-1g of hyponex V, 0.1g of inositol, 2g of peptone, 1.6g of active carbon, 7g of agar, 20g of sugar, 62.5g of banana, 0.5g of potassium nitrate, 0.5g of ammonium nitrate, 0.25mg of indolebutyric acid, 0.25-0.5mg of naphthylacetic acid and 0.25mg of rooting powder; the pH value of the nutrient solution is adjusted to 5.6. The formula is convenient to prepare; the transferring steps are reduced; the pharmaceutical costs and the artificial costs are reduced; the pollution rate is reduced; the rate of seedling emergency is high; the practical effect is good; the tissues begin to sprout in 2 months after inoculating and begin to strike root in 3 months, and the tissues are cultured to normal tissue culture seedlings after 4 months; the rate of seedling emergency is greater than 96%; each tissue culture seedling is relatively strong; after transplanting the tissue culture seedlings for 2 months, the counted survival rate reaches more than 95%, so that the production period of the roxburgh anoectochilus terminal bud seedlings is shortened, thus facilitating quick breeding of tissue culture seedlings in factories.
Owner:杭州虹越花卉有限公司

Multidirectional disturbance type device for preparation of roxburgh anoectochilus terminal bud

The invention discloses a multidirectional disturbance type device for preparation of roxburgh anoectochilus terminal bud, which comprises a drying shell body; the drying shell body closing to left and right side walls is provided with a left installing block and a right installing block; the upsides of the left installing block and the right installing block are fixedly connected to a flexible device; the flexible device comprises a supporting pillar which is arranged vertically, and the supporting pillar crosses through the drying shell body; a groove with a downwards opening is arranged onthe supporting pillar, and the inner top part of the groove is fixedly connected with a spring; the lower end of the spring is fixedly connected with a rack, one side of the rack is meshed with a gear; the gear is fixedly connected with a rotary shaft, and the rotary shaft is erected on the supporting pillar; one end of the rotary shaft is fixedly connected to a motor output shaft, outsides of theleft installing block and the right installing block are provided with a left slide slot and a right slide slot; a connecting rod is arranged in each of the left slide slot and the right slide slot.The multidirectional disturbance type device is reasonable in structure design, convenient to use and high in drying efficiency; besides, the multidirectional disturbance type device is valuable to popularize and apply.
Owner:湖北金草堂药业有限公司

A roxburgh anoectochilus terminal bud-dendrobium officinale soft capsule and a preparing method thereof

A roxburgh anoectochilus terminal bud-dendrobium officinale soft capsule is disclosed. The soft capsule comprises roxburgh anoectochilus terminal bud and dendrobium officinale which are mixed according to a weight ratio of 1-1.5:2-3. The soft capsule is prepared by following steps of: mixing the roxburgh anoectochilus terminal bud and the dendrobium officinale according to the ratio, drying until the weight is constant, smashing to obtain composite medicine powder, adding distilled water into the composite medicine powder, dipping, putting into a thermostat water bath, filtering, keeping an extracting solution, adding distilled water with the same amount, repeating the above steps, combining the extracting solutions, filtering with gauze to remove impurities, centrifuging at a high speed, subjecting the liquid supernatant to rotary evaporation and concentration to obtain a concentrate liquid, adding absolute ethyl alcohol into the concentrate liquid to obtain a concentrate solution, subjecting the concentrate solution to alcohol precipitation, performing centrifugal separation to separate the ethyl alcohol and a precipitate, performing alcohol precipitation to obtain the precipitate, adding ethyl alcohol and acetone into the precipitate to wash the precipitate, subjecting the precipitate to evaporation in a boiling water bath, putting the precipitate into a drying oven, drying until the weight is constant to obtain a roxburgh anoectochilus terminal bud-dendrobium officinale polysaccharide composite, and preparing the soft capsule. The soft capsule is enhanced in medicine effects, saved in medicine materials, accurate in dosage and convenient to eat.
Owner:漳州美利德生物工程有限公司

Preparation process of roxburgh anoectochilus terminal bud concentrated solution

InactiveCN104001033ASoup color orange red brightConducive to expanding deep processing and utilizationFood preparationPlant ingredientsSeparation technologyDietary supplement
The invention discloses a preparation process of roxburgh anoectochilus terminal bud concentrated solution, which comprises the following steps of carrying out a set of processes including extracting, cooling, centrifuging, ultrafiltering, reverse osmosis concentration, sterilizing and filling on roxburgh anoectochilus terminal bud as a raw material, thereby obtaining roxburgh anoectochilus terminal bud concentrated solution. According to the invention, a water extraction method and a membrane separation technology are adopted; effective components and natural colour, aroma and taste of roxburgh anoectochilus terminal bud are reserved to a great extent; the quality of roxburgh anoectochilus terminal bud concentrated solution is ensured; the prepared roxburgh anoectochilus terminal bud concentrated solution is orange red and bright in colour, mellow in taste and pure in aroma; the preparation process is scientific and reasonable; the flow is easy to control, and therefore, the preparation process disclosed by the invention is applied to industrial production and is beneficial to expanding deep processing utilization of roxburgh anoectochilus terminal bud; scientific basis is provided for further developing roxburgh anoectochilus terminal bud series of dietary supplements; the industrial value of roxburgh anoectochilus terminal bud is greatly increased; essence of roxburgh anoectochilus terminal bud is scientifically extracted by adopting the advanced process, and therefore, the effective components and the special flavour of roxburgh anoectochilus terminal bud are reserved to a great extent.
Owner:福建仙洋洋食品科技有限公司
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