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Formula for culturing tissues with buds and stems of roxburgh anoectochilus terminal bud to seedlings by one step

A technology of tissue culture and stem segment, which is applied in the field of culture medium formula for one-step seedling growth of A. Difficult reproduction and other problems, to achieve the effect of rapid seedling cultivation in industrialized tissue culture, reduction of drug and labor costs, and convenient preparation

Active Publication Date: 2014-08-27
杭州虹越花卉有限公司
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Problems solved by technology

[0003] In recent years, as the medicinal value of clematis has been continuously explored, its market demand has increased rapidly. However, because clematis is extremely difficult to reproduce and grows slowly in the natural state, it is difficult to meet the increasing market demand. As a result, domestic scholars continue to devote themselves to the research on its artificial propagation and cultivation, aiming to explore ways to improve the reproductive coefficient of A.
Studies have shown that tissue culture and rapid propagation technology is the most suitable propagation method for artificial cultivation of Aureus aureus, but the research reports on tissue culture technology at this stage mainly focus on improving the adventitious bud induction rate and bud proliferation coefficient, etc., but relatively speaking, the time , labor, and drug costs are still high, and the output of clematis has been unable to keep up with market demand

Method used

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  • Formula for culturing tissues with buds and stems of roxburgh anoectochilus terminal bud to seedlings by one step

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] 1) Preparation medium: Prepare the medium according to the following formula for each liter of nutrient solution: Huabao No. 1g / L, Huabao No. 1g / L, Huabao No. 5 0.5g / L, inositol 0.1g / L, peptone 2g / L, activated carbon 1.6g / L, agar 7g / L, sucrose 20g / L, banana 62.5g / L, potassium nitrate 0.5g / L, ammonium nitrate 0.5g / L, indole butyric acid 0.25mg / L, naphthalene acetic acid NAA 0.25mg / L, rooting powder 0.25mg / L, pH adjusted to 5.6.

[0012] 2) Selection of explants: Choose clematis plants that grow healthily and are free from diseases and insect pests.

[0013] 3) Detoxification: (1) Cut off the roots and leaves, and then use a knife to cut off the leaf sheath and the outer leaves of the terminal buds, taking care not to hurt the axillary buds; (2) Rinse the explants with clean water; (3) Use sterile (4) Rinse 4-5 times with 75% alcohol, then soak in 75% alcohol for 1 min; (5) Soak in 84 disinfectant for 2 min; (6) Soak in 0.1% mercury solution for 1 min; (7) ) Rinse 3-...

Embodiment 2

[0018] 1) Preparation medium: Prepare the medium according to the following formula for each liter of nutrient solution: Huabao No. 2g / L, Huabao No. 1g / L, Huabao No. 5 0.5g / L, inositol 0.1g / L, peptone 2g / L, activated carbon 1.6g / L + agar 7g / L, sucrose 20g / L, banana 62.5g / L, potassium nitrate 0.5 g / L, ammonium nitrate 0.5g / L, indole butyric acid 0.25mg / L, naphthalene acetic acid NAA0.5mg / L, rooting powder 0.25mg / L, pH adjusted to 5.6.

[0019] 2) Selection of explants: Choose clematis plants that grow healthily and are free from diseases and insect pests.

[0020] 3) Detoxification: (1) Cut off the roots and leaves, and then use a knife to cut off the leaf sheath and the outer leaves of the terminal buds, taking care not to hurt the axillary buds; (2) Rinse the explants with clean water; (3) Use sterile (4) Rinse 4-5 times with 75% alcohol, then soak in 75% alcohol for 1 min; (5) Soak in 84 disinfectant for 2 min; (6) Soak in 0.1% mercury solution for 1 min; (7) ) Rinse 3-...

Embodiment 3

[0025] A control group was set up, and the conventional formula of "bud induction --- adventitious bud proliferation --- rooting culture" was selected for 3-step seedling formation, and the steps were as follows:

[0026] 1) Matching medium: Adventitious bud induction medium formula: MS+6-BA1.0mg / L+KT1.0mg / L+agar 7g / L+sugar 20g / L, cluster bud proliferation medium formula: MS+6-BA1. 0mg / L+NAA0.5mg / L+agar 7g / L+sugar 20g / L, rooting medium formula: MS+IBA0.5mg / L+activated carbon 0.5g / L+banana puree 20g / L+agar 7g / L+sugar 20g / L, PH5 .6.

[0027] 2) Selection of explants: Choose clematis plants that grow healthily and are free from diseases and insect pests.

[0028] 3) Detoxification: (1) Cut off the roots and leaves, and then use a knife to cut off the leaf sheath and the outer leaves of the terminal buds, taking care not to hurt the axillary buds; (2) Rinse the explants with clean water; (3) Use sterile (4) Rinse 4-5 times with 75% alcohol, then soak in 75% alcohol for 1 min; (5...

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Abstract

The invention provides a formula for culturing tissues with buds and stems of roxburgh anoectochilus terminal bud to seedlings for one step. Each liter of a nutrient solution comprises the following components: 1-3g of hyponex I, 1-2g of hyponex II, 0.5-1g of hyponex V, 0.1g of inositol, 2g of peptone, 1.6g of active carbon, 7g of agar, 20g of sugar, 62.5g of banana, 0.5g of potassium nitrate, 0.5g of ammonium nitrate, 0.25mg of indolebutyric acid, 0.25-0.5mg of naphthylacetic acid and 0.25mg of rooting powder; the pH value of the nutrient solution is adjusted to 5.6. The formula is convenient to prepare; the transferring steps are reduced; the pharmaceutical costs and the artificial costs are reduced; the pollution rate is reduced; the rate of seedling emergency is high; the practical effect is good; the tissues begin to sprout in 2 months after inoculating and begin to strike root in 3 months, and the tissues are cultured to normal tissue culture seedlings after 4 months; the rate of seedling emergency is greater than 96%; each tissue culture seedling is relatively strong; after transplanting the tissue culture seedlings for 2 months, the counted survival rate reaches more than 95%, so that the production period of the roxburgh anoectochilus terminal bud seedlings is shortened, thus facilitating quick breeding of tissue culture seedlings in factories.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a medium formula for one-step growth of seedlings of A. auranthus tissue culture. Background technique [0002] Clematis clematis is a plant of the orchidaceae, which is a traditional and precious medicinal material in my country. It is known as the "King of Medicine" among the people. It has become a precious flower with both ornamental value and medicinal value. [0003] In recent years, as the medicinal value of clematis has been continuously explored, its market demand has increased rapidly. However, because clematis is extremely difficult to reproduce and grows slowly in the natural state, it is difficult to meet the increasing market demand. As a result, domestic scholars continue to devote themselves to the research on its artificial propagation and cultivation, aiming to explore methods to improve the reproductive coefficient of A. Studies have shown that tissue cultu...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 江胜德郭兰付习陈溪李春燕
Owner 杭州虹越花卉有限公司
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