1578results about How to "Low pollution rate" patented technology

The invention discloses a method for cultivating phellinus igniarius. The method includes the steps that firstly, short-cut wood is directly placed in a material bag or placed in the material bag after being bundled, the two end faces of the short-cut wood or the two ends of a short-cut wood bundle are paved with cultivation media, an opening of the bag is tightened, and then a material package is acquired; secondly, the material package is sterilized, cooled and then inoculated, and then phellinus igniarius is acquired through cultivation, wherein the cultivation media are radix puerariae residues. The radix puerariae residues are used as the cultivation media, so that growth and reproduction of phellinus igniarius mycelia can be stimulated, the mycelia are stronger, germination and field planting of the phellinus igniarius mycelia are accelerated, and eventually the spawn running time can be shortened by about 15 d; in addition, invasion and infection of contaminating microorganisms can be reduced, and statistics show that the contamination rate of the material package can be reduced by 11.7% to 17.9% and the yield of phellinus igniarius can be increased by more than 15%.

The invention relates to a high-efficiency breeding method of healthy seedlings of cane, the method is proposed aiming at the problems that seed performance degenerates, yield is greatly reduced and the quality is reduced, which are caused by that the cane is taken as asexually propagated crop, propagation coefficient is low, the amount of seeds used for cane implantation is large, and after the implantation of a plurality of years, the cane is easy to be infected with a disease; improved type healthy seedlings of the cane can be cultured by nine steps of seedling mild water detoxication processing, seedling sprouting, in vitro peeling inoculation, induction culture, virus check, propagation culture, rootage culture, domesticated seedling-refining and outdoor temporary planting; the method adopts the combination of mild water detoxication and stem tip detoxication with thorough detoxication and good effect; the direct peeling inoculation of the explant does not need to be treated with disinfection processing, has low pollution rate and light browning, and is easy to generate clustering buds by induction; the propagation coefficient is high, the speed is high, the period is short; the seed performance is table, the seedling is strong; the virus detection is precise and accurate; the survival rate of the outdoor temporary planting is high and is up to more than 90 percent; the production cost is low, the efficiency is high, and the method is suitable for industrialized production.

The invention discloses a technology for carrying out fungus growing on loose tea. The technology comprises the following steps: (1) preparing a fermenting agent, namely, separating out eurotium cristatum from fuzhuan tea, and preparing to obtain the solid pure strain fermenting agent; (2) treating raw dark green tea raw material, namely, removing the impurities in the raw dark green tea, steaming, carrying out pile fermentation and then feeding tea juice; (3) carrying out steaming inoculation and fungus growing, namely, steaming the raw dark green tea with the tea juice, then immediately feeding the solid pure strain fermenting agent and inoculating; after that, putting the inoculated raw dark green tea into a fungus growing culture room, and carrying out fungus growing culture; and (4) drying and packaging, namely, drying after fungus growing, and directly packaging the dried loose tea. After the technology is adopted, the fungus growing time is shortened, the bacterial pollution rate can be reduced in the fungus growing process of the loose tea, the problems that the existing loose tea is hard in fungus growing and easy in bacterial growth can be solved, the fungus growing rate of the loose tea reaches up to 100%, and the tea is widespread in golden flowers on the surface and the granules are plump.

The invention discloses a bletilla seed tissue culture propagation method which belongs to the technical field of Chinese herbal medicine planting. The method comprises the following six steps of seed introduction and sterilization, induction culture of protocorm, enrichment culture of protocorm, induction culture of multiple shoots, enrichment culture of multiple shoots, and rooting culture. The method has the beneficial effects that seedlings generated by adopting seed tissue culture is low in pollution rate, high in differentiation rate, stable in hereditary characters, and high in active substance content. According to the method disclosed by the invention, the tissue culture propagation period of bletilla is also greatly shortened, and the tissue culture period from seeding to rooting is less than four months, so that the production time is shortened, and the production cost is lowered.

The invention provides a ganoderma lucidum cultivation method, and belongs to the technical field of cultivation of fungus for edible and medicinal use. The ganoderma lucidum cultivation method comprises choosing of a field, selection of building materials, construction of a permanent greenhouse, consolidation of sand culture bed land, isolation of contact between sand and soil in the field through plastic membranes, sand burying of ganoderma lucidum fungus sticks, laying of spraying pipelines, shading, insect prevention measures, and the like. According to the ganoderma lucidum cultivation method, standardized cultivation of ganoderma lucidum can be achieved, cultivation conditions can be controlled, traditional Chinese medicine quality management code requirements are fitted, a foundation is not needed to be moved every year, and manpower, material resources and financial resources are saved. The ganoderma lucidum fungus sticks are not contacted with the soil, so heavy metal, toxic substances and pesticide residues all can be controlled effectively. Cultivated ganoderma lucidum is stable and high in yield, namely the yield of the ganoderma lucidum is 570-580 kilograms per mu and is increased by 30% or so compared with conventional cultivation, quality is excellent, namely, competitor pollution rates of the fungus sticks and secondary entities are greatly reduced, malformation does not exist in the secondary entities, and the secondary entities cannot be polluted by water and pollutants in the soil, and the ganoderma lucidum cultivation method is popularization and application of ganoderma lucidum standardized cultivation. The ganoderma lucidum cultivation method is also suitable for edible and medicinal fungus of phellinus igniarius, mushrooms, agaric and the like.

The invention discloses a rapid tissue culture and propagation method of curcuma alsimatifolia, belonging to the technical field of plant tissue culture. The method comprises the following steps of: firstly, preparation of a culture medium; secondly, culture and sterilization of earthnut budlet; thirdly, culture of explant sterile seedling; fourthly, induction culture of adventitious bud; fifthly, propagation culture of adventitious bud; sixthly, root growing culture of plantlet; and seventhly, seedling adaptation, acclimation and transplanting of tissue culture seedling. The method disclosedby the invention has the advantages that propagation culture period is short (14-20 days), propagation coefficient is high (improved to 5-8 times from 2-4 times), the consistency of the adventitious bud is good, tissue culture breeding cycle is short (50-60 days), seedling quality is good, and transplanting survival rate can reach up to more than 95%, and is a method for efficiently and rapidly providing high-quality curcuma alsimatifolia seedlings. The method disclosed by the invention can be popularized and applied to landscaping and tissue culture seedling enterprises.

The invention relates to a method for designing a high-enthalpy arc heater with fixed arc length, which comprises the following steps that: a compression channel positioned between a positive electrode and a negative electrode of an arc heater is made into a plurality of insertion section (or slice) structures which are insulated from each other; adjacent insertion sections (or slices) are insulated from each other, and tangential rotary gas is aerated between the adjacent insertion sections (or the slices) to compress arc columns and blow over plasmas which are gathered between the sections (or the slices); the thickness of each insertion section (or slice) can be designed according to the need; and when an airflow enthalpy value is higher, the insertion sections (or the slices) have smaller thicknesses, more insertion sections (or the slices) are needed, the pressure gradient distribution of the gas which tangentially enters compression sections from the positions between the insertion sections (or the slices) is also greater, the arc columns can be compressed to be thinner and closer to the axial line, the heat exchange between the electric arc and the gas is also more sufficient, and the airflow specific enthalpy is also higher. The invention can provide the basic design method for the high-enthalpy arc heater with the fixed arc length, and can ensure that an enthalpy value of the arc heater is improved to more level.

The invention relates to a pretreatment method of Acesulfame-K, comprising the following steps: firstly conducting nitric acid digestion treatment, then conducting high-temperature ashing treatment, and finally dissolving. The pretreatment method disclosed herein is an improvement based on orginal dry ashing and wet ashing, simplified the operation process, and effectively reduces the possibility of sample contamination. The invention further provides a detection method for detecting potassium in Acesulfame-K after pretreating the Acesulfame-K with the above pretreatment method, characterized by pretreating an Acesulfame-K sample with the above pretreatment method and then conducting instrument detection of potassium, thus the precision of the detection can be raised, and the content of potassium in the original sample can be accurately reflected.

The present invention is protein feed and its preparation process with potato slag. Protein feed is prepared with waste potato slag from starch production, and through squeezing to dewater, adding certain amount of supplementary material, inoculating certain amount of microbe seed and solid fermentation. The said preparation process is simple, and has high yield, low investment, low power consumption and other advantages. Thus obtained feed product has the fermented product, including microbe, metabolite and substrate utilized fully, and possesses the active components maintained, high amino acid content and high nutritious value.

The invention belongs to the technical field of artificial cultivation of medicinal fungi, and particularly relates to a method for cultivating ganoderma lucidum karst. The method includes the steps of obtaining ganoderma lucidum karst mother species, ganoderma lucidum karst protospecies and ganoderma lucidum karst cultispecies respectively, and conducting substitute cultivation of the ganoderma lucidum karst cultispecies to obtain ganoderma lucidum karst, wherein a cultispecies culture medium is prepared from corn straw, sawdust, pig manure, cow dung, rapeseed meal, humic acid, an amillariella mellea fermented liquid, a bacillus subtilis fermentation liquid and gypsum, and a culture medium of the substitute cultivation is same as the cultispecies culture medium. According to the method, the yield of ganoderma lucidum karst sporophore and conidial powder is significantly improved, and the contents of effective components, like ganoderma lucidum karst polysaccharide and ganoderma lucidum karst total triterpenoid, are significantly improved.

The invention discloses a method for using lily to prepare parent group to cultivate grouped and cross, belonging to plant group cultivate and cross technique. The inventive method comprises that preparing cultivate medium, using non-matured blank of lily as explant to be induced, increased, expanded, cultivated, refrigerated, transplanted, and virus detected, and using harmless tube bulb seed to plant in field, flower, and cross, to obtain lots of lily harmless seeds. The invention has the advantages in simple grafting operation, low pollution, high survival rate, low variant rate, high cross purity, high seed quality, shortened induce cultivate period, reduce cost and high practicality.

A roller washing machine with a detachable inner barrel comprises a tank body; a tank body door is arranged on the tank body; an outer barrel door is arranged on an outer barrel; a magnetizer is fixed on a bottom flange of the inner barrel; a spline groove is formed in the magnetizer; the bottom of an outer barrel casing is provided with a carbon brush which supplies power for an electromagnet; a transmission mechanism which is arranged at the bottom of the outer barrel is mainly formed by a transmission shaft and the electromagnet; a sliding channel is arranged on the transmission shaft; the electromagnet is fixed at the front end of the transmission shaft; a spline is fixed on the electromagnet; the spline is connected with the spline groove. When the roller washing machine works, the electromagnet generates magnetism after being electrified, the inner barrel is adsorbed in the transmission mechanism, mutual mechanical transmission between the electromagnet and the magnetizer is correspondingly matched with axial positioning, or the transmission shaft and a groove of an inner barrel flange plate are connected in a matching mode to form into an axial positioning device, when the washing machine is required to be washed, power supply of the electromagnet is interrupted, the inner barrel is conveniently taken out to clean and sterilize the inner barrel and the outer barrel, the operation is convenient, the contamination rate is reduced, and cloth washing is clean and environment friendly.

A preparation method of an efficient culture material for hypsizigus marmoreus. The efficient culture material comprises, by weight, 10-30% of wood chip, 20-40% of cotton straw, 10-20% of wheat bran, 15-25% of rice bran, 2-10% of corn flour, 1-5% of cob, 5-10% of cottonseed casing and 0.5-1% of gypsum powder. The preparation method comprises steps of: weighing wood chip, cotton straw, wheat bran, rice bran, corn flour, cob and cottonseed casing according to the proportion and mixing; dumping a mixture into a pulverizer and crushing the mixture into particles about 120 mesh; adding gypsum powder according to the proportion, stirring in a mixer for 65 min; supplementing a proper amount of water till a water content of 64-66%; and disinfecting to realize a pH of 5.5-7.5. The invention has beneficial effect that because of employment of the above formula, the prepared culture material for hypsizigus marmoreus has low cost, low pollution rate, fast spawn running effect, and a single bottle mushroom yield similar to that of a culture material using cottonseed casing and cob, and high comprehensive benefit.

The invention provides a method for rapid tissue propagation and breeding seedling of bletilla striata seeds. The method comprises the following steps: disinfection and sterilization of the seeds, seed germination and protocorm induction, protocorm propagation, cluster bud induction and propagation, rooting culture, seedling adaptation and transplantation. The method has the benefits that the seed tissue culture is adopted, and obtained seedlings are low in pollution rate, high in differentiation, and high in content of active substances; meanwhile, through the adoption of the method, the tissue culture and propagation period of bletilla striata is greatly shortened, and the tissue culture period from seeds to rooted seedlings is only about 4 months, so that the production time is greatly shortened, and the production cost is reduced.

The invention discloses an RT-LAMP (loop-mediated isothermal amplification) rapid detection kit for cucumber green mottle mosaic virus (CGMMV) and a detection method. The method comprises the steps of extracting plant viruses RNA, detecting an RT-LAMP reaction and electrophoresis detection or fluorochrome, and determining whether a plant carries the viruses. An RT-LAMP primer is designed according to a coat protein gene conserved region of CGMMV, and an RT-LAMP technology is adopted to establish a detection technology which can rapidly and sensitively detect the cucurbitaceous plant CGMMV such as cucumbers, watermelons and calabashes with high specificity and an application method thereof in diagnosing disease. The detection method provided by the invention can utilize the rapid extraction RNA and conventional RNA extraction as an RNA template for an RT-LAMP experiment. The method is utilized to rapidly identify a CGMMV plant sample, the targeted quarantine inspection protective measure is adopted, and the loss caused by the disease is reduced.

The invention provides a method for cultivating plant tissue under non-aseptic condition, wherein the whole procedure of plant tissue cultivation is conducted under the natural ambient condition of non-asepsis, the method consists of adding disinfection and bactericidal agent into the culture media, preparing bacterium suppression culture media, inoculating under the natural ambient condition of non-asepsis, and placing the culture material in natural ambient condition meeting plant tissue culture.

The invention discloses a technology for bionic cultivation of wild ganoderma lucidum in a bamboo forest. Ganoderma lucidum is cultivated in the bamboo forest which is free of pollution, dense and abundant in rainfall. Basswood is buried under soil of the bamboo forest, and ganoderma lucidum strains are inoculated on the basswood to grow naturally. In this way, growth and development of the ganoderma lucidum are facilitated, and the ganoderma lucidum is similar to wild ganoderma lucidum in appearance.

A dividual conjugate fiber for obtaining a fiber structure excelling in denseness and bulkiness. The dividual conjugate fiber is one having a polyamide resin composition and a fiber forming polymer with no compatibility with the polyamide resin composition bonded together in the direction of fiber length, characterized in that the polyamide resin composition is composed of an aromatic polyamide and an aliphatic polyamide. Preferably, the aromatic polyamide is a nylon MXD6 polymer, and the aliphatic polyamide is a nylon 6 polymer.

The invention discloses a novel SERS substrate-based method for quantitatively testing pathogenic bacteria, and belongs to the technical field of biological detection. Silicon dioxide treated by an immunospectral marker is coated with gold nanoparticles for quantitative test of the pathogenic bacteria. Raman spectra signal monitoring data are more stable and reliable and can be greatly repeated. The method is good in test stability, high in sensitivity, high in specificity, the detection process is simple, fast, accurate and quantitative, and a wide detection interval and a low detection limit are obtained. The method is simple in operation, an SERS immune signal probe and a capture probe can be prepared in advance, the capture probe and the signal probe only need to be sequentially added to a to-be-detected sample for reaction during operation and the test is carried out at once through magnetic separation after the reaction is completed: the collection time is 30s, and then the concentration is immediately read from a standard curve, thereby achieving fast quantitatively testing. The method can meet the requirements of food safety and environment monitoring departments, and has wide practicability.

A process is provided for catalyst dehydrogenation of light paraffinic hydrocarbons using a catalyst comprising a noble metal and an intermediate pore size zeolite having a specified alkali content. The catalyst is sulfur tolerant, so that the dehydrogenation process can be carried out in the presence of sulfur or with periodic exposure to sulfur.

The invention belongs to the field of biological engineering and is aimed for solving the problem of mass scale plant diseases caused by fungus. The preparing process comprises subjecting a strong of Bacillus firmus (CGMCC No.0924) to second level fermentation culture under suitable condition, then carrying out filtering, salting out and column chromatography treatment.

The invention relates to a preparation method of pleurotus abalonus culture nourishment, which comprises the following components by weight percent: 32-45 of cotton seed hulls, 25-40 of bran, 12-19 of wood chips, 3.6-6.7 of corn flour, 3.5-4 of corn cobs, 0.5-1 of bean cake meal, 0.5-1 of beanstalk meal, 1.7-2 of colza cakes, 0.1-0.3 of oxygenation powder, 0.1-0.3 of calcium carbonate, 0.1-0.3 of bitter salt and 0.5-0.8 of monopotassium phosphate. The cotton seed hulls, the bran, the wood chips, the corn flour, rice bran meal, the corn cobs, the bean cake meal, the beanstalk meal and the colza cakes are combined together in proportion and are poured into a pulverizeer to be pulverized to be granules of about 160 meshes, after the granules are taken out, the oxygenation powder, the calcium carbonate, the bitter salt and the monopotassium phosphate are added in proportion, then the mixture is poured into the pulverizer to be pulverized for 60 min and then is taken out, water is added to the mixture in the proportion of 1:1.8, and then certified culture nourishment is obtained after all materials are piled in a fermentation tank, tightly covered by plastic cloth, compacted and fermented for 72 hours.

The invention provides multifunctional catalytic cracking strengthening additive which comprises a rare earth organic compound, heteropoly acid (and salt thereof), chemical inhibitor, solvent, and the like and has the functions of resisting oxidation and preventing from scorch, dispersing, increasing the acid center, passivating the metal, and the like. When being added into a catalytic cracking lifting pipe reactor, the multifunctional catalytic cracking strengthening additive can inhibit the generation of secondary reaction such as thermal cracking reaction, dehydrogenation condensation reaction, and the like, reduces the generation of side products of net gas, coke, hydrogen, and the like, and improves the yield coefficients of products with high added values, such as liquid gas (especially propylene), gasoline and diesel. The catalytic cracking strengthening additive has the function of metal deactivator and can replace the metal deactivator, therefore, the metal deactivator needs not to be added after the catalytic cracking strengthening additive is added. The invention has the function of resisting oxidation and preventing from scorch, can inhibit a lifting pipe and a settling vessel from being coked and prevent a reaction system from scale deposit, thereby being multifunctional catalytic cracking strengthening additive.

A cold cathode ionization manometer for measuring pressure in vacuum and operating on the inverse magnetron principle. For extending the service life of the manometer it is provided with two independently operating cathodes and a common anode mounted in a measuring tube. The first cathode is mounted at the inlet of the measuing tube and its discharge serves as a gas purification device which prevents the formation of contaminating layers by cracking and polymerizing hydrocarbons and other substances entering the measuring tube thereby protecting the second cathode and extending the overall service life of the manometer.

The invention provides a method for isolating and culturing liver primary cells. The method comprises the following steps: (1) inputting perfusate from hepatic portal vein of a liver; (2) inputting collagenase perfusate; (3) soaking the maturely digested liver in the collagenase perfusate; (4) adding a cleaning solution to stop digestion, filtering, and collecting hepatic cell suspension; and (5) centrifuging, discarding supernatant, resuspending with complete medium, and culturing. The operation of the method provided by the invention is simple, is low in cost and is stable and efficient, and the hepatic cells are high in yield, high in vitality and easy for adherence. The method can be generalized in laboratories, and is a conventional method for scientific research.

The invention provides an antigen-specific T lymphocyte freezing medium, comprising freezing medium A and freezing medium B; the freezing medium A includes, by volume, 30-40% of plasmalyte electrolyte injection, 30-40% of glucose and sodium chloride injection, 5-15% of dextran glucose injection and 15-25% of human albumin solution; the freezing medium B includes, by volume, 20-30% of plasmalyte electrolyte injection, 20-30% of glucose and sodium chloride injection, 5-15% of dextran glucose injection, 15-25% of human albumin solution and 10-20% of dimethyl sulfoxide; the freezing medium A and the freezing medium B are stored separately; in use, the freezing medium A and the freezing medium B are mixed in a ratio of 1:(0.5-2), forming the antigen-specific T lymphocyte freezing medium. The antigen-specific T lymphocyte freezing medium is capable of enabling less crystal to form in cells, increasing cell survival rate and maintaining tumor-killing ability of cells. The invention also provides a preparation method and application of the antigen-specific T lymphocyte freezing medium and antigen-specific T lymphocyte injection.

The invention relates to a method for earthed cultivation and strain production of Pleurotus tuberregium and a culture medium. The method provided by the invention comprises the following steps of: directly placing sclerotium tissues on a slant culture medium to carry out a strain production; culturing and cultivating by a solid culture medium and then utilizing an earthed cultivation. The method solves the problem that the Pleurotus tuberregium is cultivated by liquid strains or a bag type fruiting method for long time. The slant culture medium is composed of 35-40 parts of corn starch, 15-25 parts of wheat bran, 8-12 parts of cane sugar, 8-12 parts of glucose, 1.8-2.2 parts of monopotassium phosphate, 0.9-1.1 parts of magnesium sulfate, 5-7 parts of yeast powder, 0.008-0.012 part of VB1, 16-20 parts of agar and 800-1200 parts of water. The method provided by the invention is convenient for large-area production, the mycelium is thick and strong, the pollution rate is less than 5%, and the time for obtaining the mycelium only needs 7 days; the husk of the solid culture medium has good air permeability, the pH value can be adjusted by lime, and the solid culture medium satisfies mineral requirements; and the biological conversion rate of the earthed cultivation method can reach more than 80%.

The invention discloses a packed bed type cell bioreactor without a stirrer and a method for cultivating animal cells. To meet the requirements for large-scaled high-density animal cell cultivation, the cell bioreactor is required to amplify and strengthen functions thereof. The packed bed type cell bioreactor without the stirrer consists of a reactor body (110), wherein the reactor body is connected with a shaker (50) through an eccentric wobble plate (58); and the shaker shakes the eccentric wobble plate to make the reactor body rotate eccentrically. The upper part of the reactor body is shaped as a round table (100), and the lower part of the reactor body is a cylinder (113); a flow guide tube (130) is arranged on the upper part of the round table, an inlet (131) of the flow guide tubeis close to the inner wall of the reactor body, an outlet (133) of the round table enters a central hopper (160) along the tangential direction of the central hopper; the reactor body has a reactor lid (140) with an air inlet, an air outlet, a liquid inlet and a liquid outlet; and a packed bed (120) is arranged in the reactor body (120). The packed bed type cell bioreactor without the stirrer provides a good environment for cell cultivation.

The invention provides a novel method for preparing a third-level strain of pleurotus eryngii. The method combines traditional processes for a first-level strain, a second-level strain and a branched strain and is suitable for production of the pleurotus eryngii and other wood rotting edible fungus strains. The processes include inoculation from the first-level strain and the second-level strain to the three-level branched strain and then to a cultivating bag. A method for preparing the third-level strain of the pleurotus eryngii by using a poplar branch culture medium and a bridging material composed of broad-leaved wood sawdust, wheat bran and calcium carbonate is adopted. The method provided by the invention has the characteristic that branch is adopted for inoculating the third-level strain to the cultivating bag; the strain production process has the advantages of easiness for inoculation operation, high speed and low pollution rate; and the production cost is reduced and the production efficiency is improved by using the production process.

The invention provides a method for cultivating black funguses in a three-dimensional mode in a greenhouse, and relates to a cultivation method of edible mushrooms. The problems that in the hanging bag cultivation technology, fungus seeds are not properly selected, arrangement density is large, ventilation is difficult, the fungus making requirement is high, black fungus slices can not open, and the temperature and humidity of the greenhouse can not be easily controlled are solved. The method for cultivating the black funguses comprises the steps of selecting fungus seeds, building up the greenhouse, disinfecting the inside of the greenhouse, producing fungus bags, sterilizing, inoculating funguses, growing funguses, managing opening and fungus slice appearing, hanging bags, managing accelerating germination, managing the fungus slice growth period, managing collection and humidification turning, and picking top fungus slices when fungus bags fall down on the ground. The method has the advantages that fruit bodies are good in economic character, free of pollution, organic and watery, picking is easy and practical, and management efficiency is high.