1577results about How to "Low pollution rate" patented technology

The invention discloses a bletilla seed tissue culture propagation method which belongs to the technical field of Chinese herbal medicine planting. The method comprises the following six steps of seed introduction and sterilization, induction culture of protocorm, enrichment culture of protocorm, induction culture of multiple shoots, enrichment culture of multiple shoots, and rooting culture. The method has the beneficial effects that seedlings generated by adopting seed tissue culture is low in pollution rate, high in differentiation rate, stable in hereditary characters, and high in active substance content. According to the method disclosed by the invention, the tissue culture propagation period of bletilla is also greatly shortened, and the tissue culture period from seeding to rooting is less than four months, so that the production time is shortened, and the production cost is lowered.

The invention provides a ganoderma lucidum cultivation method, and belongs to the technical field of cultivation of fungus for edible and medicinal use. The ganoderma lucidum cultivation method comprises choosing of a field, selection of building materials, construction of a permanent greenhouse, consolidation of sand culture bed land, isolation of contact between sand and soil in the field through plastic membranes, sand burying of ganoderma lucidum fungus sticks, laying of spraying pipelines, shading, insect prevention measures, and the like. According to the ganoderma lucidum cultivation method, standardized cultivation of ganoderma lucidum can be achieved, cultivation conditions can be controlled, traditional Chinese medicine quality management code requirements are fitted, a foundation is not needed to be moved every year, and manpower, material resources and financial resources are saved. The ganoderma lucidum fungus sticks are not contacted with the soil, so heavy metal, toxic substances and pesticide residues all can be controlled effectively. Cultivated ganoderma lucidum is stable and high in yield, namely the yield of the ganoderma lucidum is 570-580 kilograms per mu and is increased by 30% or so compared with conventional cultivation, quality is excellent, namely, competitor pollution rates of the fungus sticks and secondary entities are greatly reduced, malformation does not exist in the secondary entities, and the secondary entities cannot be polluted by water and pollutants in the soil, and the ganoderma lucidum cultivation method is popularization and application of ganoderma lucidum standardized cultivation. The ganoderma lucidum cultivation method is also suitable for edible and medicinal fungus of phellinus igniarius, mushrooms, agaric and the like.

The invention discloses a technology for bionic cultivation of wild ganoderma lucidum in a bamboo forest. Ganoderma lucidum is cultivated in the bamboo forest which is free of pollution, dense and abundant in rainfall. Basswood is buried under soil of the bamboo forest, and ganoderma lucidum strains are inoculated on the basswood to grow naturally. In this way, growth and development of the ganoderma lucidum are facilitated, and the ganoderma lucidum is similar to wild ganoderma lucidum in appearance.

The invention provides an antigen-specific T lymphocyte freezing medium, comprising freezing medium A and freezing medium B; the freezing medium A includes, by volume, 30-40% of plasmalyte electrolyte injection, 30-40% of glucose and sodium chloride injection, 5-15% of dextran glucose injection and 15-25% of human albumin solution; the freezing medium B includes, by volume, 20-30% of plasmalyte electrolyte injection, 20-30% of glucose and sodium chloride injection, 5-15% of dextran glucose injection, 15-25% of human albumin solution and 10-20% of dimethyl sulfoxide; the freezing medium A and the freezing medium B are stored separately; in use, the freezing medium A and the freezing medium B are mixed in a ratio of 1:(0.5-2), forming the antigen-specific T lymphocyte freezing medium. The antigen-specific T lymphocyte freezing medium is capable of enabling less crystal to form in cells, increasing cell survival rate and maintaining tumor-killing ability of cells. The invention also provides a preparation method and application of the antigen-specific T lymphocyte freezing medium and antigen-specific T lymphocyte injection.

The invention discloses a packed bed type cell bioreactor without a stirrer and a method for cultivating animal cells. To meet the requirements for large-scaled high-density animal cell cultivation, the cell bioreactor is required to amplify and strengthen functions thereof. The packed bed type cell bioreactor without the stirrer consists of a reactor body (110), wherein the reactor body is connected with a shaker (50) through an eccentric wobble plate (58); and the shaker shakes the eccentric wobble plate to make the reactor body rotate eccentrically. The upper part of the reactor body is shaped as a round table (100), and the lower part of the reactor body is a cylinder (113); a flow guide tube (130) is arranged on the upper part of the round table, an inlet (131) of the flow guide tubeis close to the inner wall of the reactor body, an outlet (133) of the round table enters a central hopper (160) along the tangential direction of the central hopper; the reactor body has a reactor lid (140) with an air inlet, an air outlet, a liquid inlet and a liquid outlet; and a packed bed (120) is arranged in the reactor body (120). The packed bed type cell bioreactor without the stirrer provides a good environment for cell cultivation.