233 results about "Auxin" patented technology

The invention discloses a growth-promoting bacillus velezensis FSUXF-717 capable of tolerating heavy metals and application of the growth-promoting bacillus velezensis FSUXF-717, and belongs to the technical field of agricultural microorganisms. The bacillus velezensis FSUXF-717 has the following advantages: (1) the bacillus velezensis FSUXF-717 has multifunctional metabolic characteristics: various carbon sources such as cane sugar, raffinose and aesculin can be utilized, and the bacillus velezensis FSUXF-717 has the functions of producing long-chain hormone, dissolving phosphorus, secreting ACC deaminase, efficiently forming a biological membrane and the like; (2) broad-spectrum adaptability: the strain has strong tolerance to heavy metal pollution (Cd / Pb / Cr / Ni), drought and broad-spectrum acid-base stress (pH 4.5-9.5); under the condition of relatively low bacterium concentration, the auxin can be stably synthesized in a heavy metal-acid-base composite stress environment. And (3) the activity of soil sucrase and soil phosphatase is enhanced. Excavation of the strain can provide an important germplasm resource basis for development and utilization of novel efficient microbial fertilizers and lay a foundation for green and high-quality development of agriculture.

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

The invention provides a Kitasatospora xanthophycea JY01 strain and application thereof, the strain is separated from intestinal tracts of spring silkworms, has good disease-resistant and growth-promoting effects, and is specifically expressed in that cellulase, beta-1, 3-glucanase, siderophore and auxin IAA can be secreted; pot experiments show that the biocontrol efficiency of the microbial inoculum to wheat stalk rot is 57.90%, the biocontrol efficiency of the microbial inoculum to corn stalk rot is 46.41%, and the plant height and fresh weight are both significantly increased. The microbial agent is expected to be applied to preparation of biological bacterial fertilizer for effectively preventing and controlling soil-borne fungal diseases.

Herbicidal compositions comprising a herbicidal malonamide (Component A), selected from a compound of formula (I) and an auxin mimic (Component B).

The invention provides bacillus subtilis KC-1 as well as a composition and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacillus subtilis KC-1 is CGMCC (China General Microbiological Culture Collection Center) No.35237. The bacillus subtilis KC-1 can secrete IAA, has nitrogen fixation capacity and can generate siderophores. The bacillus subtilis KC-1 and whey protein are used as a composition, and the bacillus subtilis KC-1 and the whey protein jointly act to prepare the seed treatment material, so that a synergistic effect can be achieved, and corn seed germination and seedling root development are remarkably promoted. Experiments show that compared with single use of the whey protein and the bacillus subtilis KC-1, the combined use of the whey protein and the bacillus subtilis KC-1 can significantly improve the seed germination rate, the length of buds on the overground part, the length of main roots on the underground part, the total root volume, the total root surface area, the total root length and the seedling auxin concentration, and also can significantly reduce the concentration of abscisic acid in seedlings, so that the survival rate of the seedlings is improved. The germination of seeds and the growth of seedlings under drought conditions are promoted, and the drought resistance of plants is improved.

The invention belongs to the field of microorganisms, and particularly discloses a microbacterium paraoxidans strain and application thereof, the microbacterium paraoxidans strain TYQ7 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.27216, and the preservation number is CGMCC No.27216. The microbacterium paraoxidans strain TYQ7 has the advantages that the microbacterium paraoxidans strain TYQ7 is preserved in the China General Microbiological Culture Collection Center (CGMCC); the applicant finds that the microbacterium paraoxidans can secrete auxin, can promote growth of cucumber seedlings, also has the capabilities of interfering tropism migration of nematodes and killing nematodes, has the effect of remarkably relieving stress of root-knot nematodes, also finds that the microbacterium paraoxidans has the capability of inhibiting normal growth of fusarium oxysporum, and has the effect of remarkably relieving stress of root-knot nematodes. Furthermore, the microbacterium paraoxidans have the capability of degrading vegetable straws and the capability of dissolving phosphorus, so that the applicant considers that the microbacterium paraoxidans have a remarkable application prospect in the fields of agriculture and environmental protection.

The invention belongs to the field of agricultural microorganisms, and particularly relates to coxakella cocori and application thereof. The coxakia cokei is preserved in the China General Microbiological Culture Collection Center, the classification name of the coxakia cokei is HHQGLZ30-1 (Kosakia cokei HHQGLZ30-1), the preservation date of the coxakia cokei is January 29, 2024, and the preservation number of the coxakia cokei is CGMCC No.29798. The coxakia cokei is named as the coxakia cokei HHQGLZ30-1. The coxakella cocori can secrete auxin, has nitrogen fixation capacity and siderophore production capacity, can convert iron elements which are difficult to absorb and utilize by plants in soil into siderophore chelated iron, and can promote plant growth and antagonize phytopathogens. By applying the growth-promoting and disease-preventing microbial agent prepared from the Corisakia cocori HHQGLZ30-1, the stress resistance of crops can be effectively improved, the growth of the crops is promoted, the crops are healthier, and the leaf surfaces are brighter and greener.

The invention relates to the technical field of microbial fermentation, and discloses an agricultural ecological probiotic complex microbial inoculant, which is prepared from the following components in percentage by mass: 30 to 35 weight percent of beneficial microbial inoculant, 20 to 25 weight percent of mineral source potassium fulvate, 15 to 20 weight percent of slow release agent, 10 to 15 weight percent of nano polypeptide fermentation liquor and the balance of carrier, the processing method comprises the following steps: S1, pretreating the raw materials; s2, preparing nano polypeptide fermentation liquor; s3, complex microbial inoculants are compounded; and S4, drying and forming. According to the invention, the mineral source potassium fulvic acid and the nano-polypeptide fermentation liquor are added, so that effective regulation and control of soil micro-ecology are realized; crop growth is promoted through cooperation of multiple strains, growth hormone is secreted through bacillus subtilis and the like, nitrogen is fixed through bacillus amyloliquefaciens, root development is stimulated in cooperation with mineral source potassium fulvic acid, and the plant height, stem diameter and fresh weight of crops are remarkably increased; the particle size of the polypeptide subjected to high-pressure homogenization treatment is 80-120nm, the absorption efficiency is improved by 60%, and a crop disease-resistant signal channel is activated.

The present disclosure provides compositions and methods for altering auxin accumulation in corn or maize plants. Methods and compositions are also provided for altering the expression of genes related to auxin efflux through editing or mutagenesis of a brachytic2 (br2) gene to introduce a premature stop codon or a deletion into the gene such that a truncated Br2 protein encoded by the mutant allele of the br2 gene, which may be a dominant or semi-dominant allele, has at least part of a transmembrane domain without a nucleotide binding domain or motif. Modified plant, plant parts and cells having such a mutant allele with reduced or altered expression or activity of a br2 gene product can have improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types in the plant.

The invention discloses mortierella alpina GRB-1, a fungicide and application of the fungicide, and belongs to the technical field of agricultural microorganisms. The Mortierella alpina GRB-1 is separated from root systems of healthy fruit trees in a continuous cropping apple orchard for the first time, and the Mortierella alpina GRB-1 has the capabilities of producing protease, producing siderophores, dissolving phosphorus, producing ammonia gas and producing auxin, can inhibit fusarium and has the application potential of relieving continuous cropping obstacles. The product prepared by combining the straw with crop straws has an excellent effect on relieving apple continuous cropping obstacles, can promote the growth of overground parts and underground parts of continuous cropping apple trees, improves the relative abundance of bacterial communities in apple continuous cropping soil and the nutrient content in the apple continuous cropping soil, improves the root activity of apples, and improves the survival rate of the apples. The method can be used for preventing and treating apple successive cropping obstacles.

The invention belongs to the technical field of microorganisms, and discloses saline-alkaline tolerant halomonas and application thereof. The Franzmannia qiaohouensis is separated from rhizosphere soil of broom corn millet in saline-alkali soil, the strain number is STM-122, the preservation number is CGMCC No.36349, the classification status is clear through 16S rRNA (ribosomal ribonucleic acid) gene sequence identification and phylogenetic analysis, and the Franzmannia qiaohouensis has the functions of dissolving phosphorus and producing EPS (expandable polystyrene), auxin and siderophore. Through bacterial suspension seed soaking treatment, the germination potential and germination rate of broom corn millet seeds under saline-alkali stress can be remarkably improved, and the indexes of seedling root length, plant height and the like are promoted to be improved; meanwhile, the invention provides a complete preparation method of the bacterial strain fermented bacterial fertilizer, and the viable count of the obtained fermented bacterial fertilizer is greater than or equal to 1 * 10 < 8 > CFU / g. The method can enrich salt-tolerant microbial resources, and provides technical support for planting broom corn millet and other crops in saline-alkali soil.

The invention relates to the technical field of plants, in particular to application of surfactin in promotion of rape root development. According to the invention, a new function of the surfactin is found, and the surfactin can be used as a signal molecule to promote expression of related genes of auxin of the rape and promote development of lateral roots of the rape and formation of a branch prosite, so that development of a root system of the rape is remarkably promoted. Surfactin can be used for preparing products for promoting root development of rape, such as plant growth regulators, fertilizers or fertilizer additives and the like, and has a wide application prospect.

The invention relates to a compound microbial fertilizer containing a seaweed extract as well as a preparation method and application of the compound microbial fertilizer, and aims to solve the problems that a traditional embedding material in a microbial fertilizer provided by the prior art is easy to disintegrate in a high-salt environment, a microbial inoculum is easy to inactivate, and the effect of the fertilizer in a high-pH environment is relatively small. Adding a seaweed extract and tannic acid into the bacterial liquid to form microcapsules, taking seaweed oligosaccharide and humic acid composite liquid as an outer slow-release layer, and spraying the bacterial liquid onto the surfaces of fertilizer particles through a nozzle, so that the fertilizer forms a gradient coating structure. According to the preparation method of the compound microbial fertilizer containing the seaweed extract, the fertilizer forms an ion barrier layer in a high-salt environment, natural auxin and humic acid in the seaweed extract synergistically activate strain metabolism, and after the prepared compound microbial fertilizer is applied, the growth effect of crops in a high-pH environment can be greatly improved.

The invention discloses a PagARF16.1 gene for regulating xylem development of poplar and application of the PagARF16.1 gene, and belongs to the technical field of biological genetic engineering. A nucleotide sequence and an amino acid sequence of a coding region of the PagARF16.1 gene are respectively shown as SEQ ID NO. 3 and SEQ ID NO. 4. The PagARF16.1 gene is knocked out by utilizing a CRISPR / Cas9 technology, and an experimental result shows that compared with a wild type poplar, the plant height of the mutant poplar is remarkably reduced, and xylem is remarkably reduced. The result shows that the poplar auxin response factor gene PagARF16.1 is a key regulatory factor for regulating xylem development. The invention provides a new technical means for regulating and controlling the xylem development of the poplar, and has an important forest tree genetic engineering application value.

The invention belongs to the technical field of cultivation and utilization of herba artemisiae annuae, and particularly relates to a method for increasing the breeding success rate of herba artemisiae annuae seeds in alpine regions. The method comprises the following steps: cleaning the fluffy wormwood seeds, then adding the fluffy wormwood seeds into an acid solution, carrying out acid soaking treatment, and then washing the fluffy wormwood seeds to obtain the fluffy wormwood seeds subjected to acid soaking; the method comprises the following steps: adding the acid-soaked artemisia splendens seeds into somatotropin, carrying out growth promoting treatment, washing with water, treating the seeds with alcohol and sodium hypochlorite in sequence, and finally carrying out seed germination treatment. According to the method, through the synergistic effect of the steps, the germination rate of the artemisia splendens seeds can be remarkably increased, the germination time lag is greatly shortened, robust growth of seedlings is promoted, and the problem of large-scale propagation of the artemisia splendens seeds is effectively solved.

The present application belongs to the field of agricultural technology, and particularly relates to a composition for improving salt tolerance of plants and application thereof. The present application applies auxin (in the form of aqueous solution or powder), an aqueous solution of auxin and proline, an aqueous solution of auxin and betaine, an aqueous solution of auxin-proline-betaine ternary complex to plants by foliar spraying or root irrigation. Experiments have proved that auxin can quickly promote the sodium ion efflux of plants, reduce the sodium ion content in plants, and significantly improve the salt tolerance; the above-mentioned complex treatment can produce a synergistic effect, and further improve the growth of plants in a high-salt environment. The composition provided by the present application can be used as a plant salt tolerance promoter in field production, has a remarkable effect on enhancing the adaptability and stress resistance of plants to salt-affected land, has strong practicability, and has good popularization and application value.

The invention discloses a fertilizer for promoting and improving the immunity of plants and a preparation method, and relates to the field of fertilizers. The preparation method comprises the following steps: mixing, smashing and grinding earthworms, eucalyptus leaves, wheat leaves and hawthorn leaves, putting into an ethanol solution, oscillating for 20-24 hours in a shaking table, and centrifugally filtering to obtain an active extracting solution; adding a sodium alginate solution into the suspended bacteria solution and the active extracting solution, and slowly adding carboxymethyl chitosan and a calcium chloride solution; and preparing. According to the invention, the plant growth-promoting rhizobacteria are fixed in the composite hydrogel, and the composite hydrogel can promote colonization of strains at plant root tips and lateral roots so as to regulate deposition of endothelial cell suberite, optimize mineral nutrition balance and abiotic stress response of plants and improve accumulation of auxin at the root tips; meanwhile, an active extracting solution is added into the composite hydrogel, immune response can be activated, and the metabolic activity and antioxidant activity of plants are greatly improved in combination with extracts of other substances.

The invention provides a nutrition regulator based on bioactive substances and a preparation method of the nutrition regulator based on the bioactive substances. Comprising the following raw materials in parts by weight: 10-20 parts of zymosan, 15-25 parts of fulvic acid, 20-30 parts of a high-activity carbon source, 5-10 parts of compound biological enzyme, 8-15 parts of algae auxin, 8-12 parts of compound probiotics, 3-8 parts of betaine, 15-25 parts of compound mineral elements and 5-10 parts of compound trace elements. According to the nutrient regulator, the algae auxin is formed by compounding the undaria pinnatifida fermentation product, the pomegranate peel fermentation product and the enteromorpha fermentation product, the undaria pinnatifida fermentation product promotes diatom growth, the enteromorpha fermentation product promotes green algae growth, the pomegranate peel fermentation product can inhibit growth of harmful algae such as blue-green algae, and the nutrient regulator has a synergistic effect on the algae auxin. Therefore, the proportion of beneficial algae such as diatom is increased and maintained for a long time, stable, efficient and high-quality water color beneficial to culture is formed, and the productivity structure of the pond is fundamentally optimized.

The invention discloses an active polypeptide of protoporphyrinogen oxidase and application, the polypeptide has at least 85%, 90%, 95%, 99% and 100% sequence identity of an amino acid sequence as shown in one of SEQ ID NO: 1-42, and has protoporphyrinogen oxidase activity. The active polypeptide disclosed by the invention shows relatively high tolerance to PPO inhibitor herbicides, and has a wide application prospect in plants. According to the weed control method provided by the invention, the occurrence of resistant weeds can be effectively reduced, and transgenic crops obtained by the method can resist various herbicides such as PPO inhibitors, ACC enzyme inhibitors, ALS inhibitors, EPSPS inhibitors and synthetic auxin at the same time, and have broad-spectrum herbicide resistance; in the weed control process, the PPO inhibitor herbicide is used in combination with one or more other herbicides, so that the cost can be effectively reduced, the control efficiency can be improved, and the weed resistance can be greatly avoided.

The application discloses an application method of leguminous green manure combined with growth-promoting bacteria for emission reduction and efficiency increase, and relates to the technical field of crop planting. The method comprises the following steps: sowing black bean seeds in soil inoculated with bacillus velezensis, and culturing black bean plants to the full flowering stage, and then crushing and turning over the black bean plants as green manure. A special bacillus velezensis is separated, which has the effects of bacteriostasis, nitrogen fixation, phosphorus dissolution, potassium solution, biofilm formation and auxin production, and has excellent biological characteristics such as stress resistance, rapid growth and easy separation, and the advantages of easy cultivation, safety, harmlessness and no pollution. Meanwhile, the bacillus velezensis can ferment to produce rich metabolic products, has good antibacterial activity and stress resistance. The bacillus velezensis is particularly suitable for being combined with black beans for green manure cultivation, and can maximize the effects of soil fertilization and gas emission reduction of green manure.

The invention discloses an application of an indica rice OsRNU2 gene. The registration number of the nucleotide sequence of the OsRNU2 gene in Genbank is LOC4324161, and the registration number of the nucleotide sequence of the OsRNU2 gene in Genbank is LOC4324161. The difference of the indica rice OsRNU2 gene promoter is the reason for the utilization efficiency and yield of indica rice and japonica rice nitrogen fertilizer, and the indica rice OsRNU2 gene promoter sequence is shown as SEQ ID NO.2. In the background of Huajing indica 74, 159 parts of materials carrying IRAT261 single fragment replacement lines are used for QTL (quantitative trait loci) analysis, and the site qRNU2 influencing the nitrate nitrogen absorption rate is positioned. The OsRNU2 haplotype expression level in indica rice is low, so that the indica rice can accumulate more auxin. The excellent allelic variation of the OsRNU2 in the indica rice is introduced into the japonica rice, so that the nitrogen fertilizer utilization efficiency and yield of the japonica rice can be improved.

The invention discloses application and a method of an iaaM gene in creating a new chrysanthemum flower type, and belongs to the field of plant genetic engineering and ornamental plant molecular breeding. The complete CDS sequence of the iaaM gene derived from agrobacterium tumefaciens is constructed into a plant expression vector and is introduced into a chrysanthemum plant, so that stable overexpression of iaaM in a chrysanthemum body is realized. By improving the level of endogenous auxin of plants, the corolla tube fusion of the ligulate flowers is obviously promoted, the flat petal flower is changed into the key petal flower, and the directional improvement of the flower is realized. Compared with traditional cross breeding, the method has the advantages of being short in breeding period, low in genetic background complexity, controllable in target character and the like, and an effective molecular breeding strategy is provided for precise regulation and control of chrysanthemum flower types.

The application discloses application of a rice OsARF24 gene. The nucleotide sequence of the OsARF24 gene has an accession number of LOC4352211 in Genbank. The rice OsARF24 gene is a key element in an auxin signal pathway. Knocking out the OsARF1 can cause the expression amount of nitrogen metabolism related genes of the rice, the nitrate nitrogen absorption rate, the plant height and the yield of the rice to be reduced, and the nitrogen fertilizer utilization efficiency to be reduced in a comprehensive manner. The function of the OsARF24 gene in positively regulating the nitrogen fertilizer utilization efficiency of the rice is clarified, and a technical route for increasing the nitrogen fertilizer utilization efficiency of the rice by increasing the expression of the OsARF24 in the rice is provided.

The application discloses Zanthoxylum bungeanum miR167b The application belongs to the technical field of genetic engineering, and discloses a gene in the application of regulating plant growth and development miR167b The application constructs an expression vector of the gene, and makes the gene overexpress in tomatoes, and finds that miR167b The overexpression plant is dwarfed, the shape of the fruit is changed, the size and weight of the fruit are significantly lower than those of the wild type, the fruit setting rate is high, the fruit is seedless, the number of fruit chambers is reduced, ovule abortion occurs, and no embryo sac is formed in the interior. Further analysis finds that the indole butyric acid content in the fruit of the overexpression plant is increased, which indicates that miR167b The gene can regulate auxin to induce fruit apomixis, lays a foundation for revealing the apomixis mechanism of Zanthoxylum bungeanum, and provides gene resources and basis for improving the breeding strategy of woody plants, and has important theoretical value and application prospect.

The present invention belongs to the field of microalgae cultivation technology, and discloses a method for promoting microalgae carbon fixation and oil production by using plant hormones and saline wastewater. Microalgae are cultivated in a wastewater culture medium to which sodium chloride and plant hormones are added. The plant hormones include at least abscisic acid, which can effectively alleviate salt stress and promote microalgae oil accumulation and growth. Plant hormones also include one of auxin and gibberellin. When abscisic acid acts synergistically with auxin or gibberellin, it is more helpful to improve the oil yield of microalgae. In addition, sodium chloride, abscisic acid, 4-amino-2-methylthiopyrimidine-5-carboxamide and 4-methyl-5-thiazoleethanol can also be added to the wastewater to synergistically enhance microalgae biomass and oil accumulation. The present invention provides an effective way to reduce the cost of microalgae cultivation and realize the industrial production of microalgae biodiesel, with both environmental and energy benefits.

The application discloses a plant-source bio-growth-promoting synergistic fertilizer and a preparation method thereof. The fertilizer is prepared by compounding plant-source composite growth-promoting substances, a composite microbial flora and a carrier material in a specific mass ratio. The plant-source composite growth-promoting substances are prepared by solid-state fermentation and extraction of gynostemma pentaphyllum, astragalus and acanthopanax. The composite microbial flora contains brazilian nitrogen-fixing spirillum, fluorescent pseudomonas and brevibacillus brevis, and is embedded and fixed by using kappa-carrageenan microcapsules containing biochar nanoparticles. The carrier is lignosulfonate-modified nano-attapulgite. The application also provides a corresponding preparation method. The granular fertilizer is finally prepared through steps of activating the strains by a composite activation solution, embedding by microencapsulation and carrier loading granulation. The fertilizer combines the synergistic effect of plant-source active substances and functional microorganisms, significantly improves the activity of the flora and the field colonization capacity, and can effectively promote plant growth, improve crop yield and quality.

The invention discloses a nutrient solution for cultivating cynara plantago, and belongs to the technical field of plant cultivation, the nutrient solution comprises a Hoagland nutrient solution, auxin and a plant growth retardant, the concentration of the auxin is 0-0.01 mg / L, and the concentration of the plant growth retardant is 0.005-0.01 mg / L. According to the method, the problem that the survival rate of the plantain thistle seedlings is low is solved, the overall growth of the plantain thistle seedlings is effectively promoted, the plantain thistle seedlings with developed root systems and robust plant types are efficiently cultivated, and therefore the survival rate of transplanting is remarkably increased. The invention further provides a seedling raising method for cultivating the cynara plantaginis.

This invention relates to a gene, MaYUCCA2, that promotes adventitious root development and its applications, belonging to the field of plant growth and development genetic engineering. The nucleotide sequence of the MaYUCCA2 gene is shown in Sequence 3; its amino acid sequence is shown in Sequence 4. This invention, by transferring MaYUCCA2 into *Populus alba*, produces transgenic poplar trees named MaYUCCA2-OE. Compared with non-transgenic poplar trees (WT), the results show that MaYUCCA2-OE has a significantly higher endogenous auxin content, and during asexual propagation tissue culture, adventitious roots are more easily formed and their number is significantly increased. Furthermore, the callus tissue of MaYUCCA2-OE transgenic poplar is more significantly enlarged and deeper red in color compared to WT, and it is more likely to induce the production of adventitious buds.

The present invention discloses a strain of Mortierella alpina GRB-1, a microbial agent and its application, belonging to the field of agricultural microbiology. The present invention is the first to isolate a strain of Mortierella alpina ( Alpine Mortierella ) GRB‑1, a strain that simultaneously produces proteases, siderophores, phosphate solubilization, ammonia, and auxin, can inhibit Fusarium spores and has the potential to alleviate continuous cropping problems. Combining it with crop straw to create a product has shown excellent results in alleviating continuous cropping problems with apples. It can promote the growth of both above- and below-ground parts of continuously cropped apple trees, increase the relative abundance of bacterial communities and nutrient content in soils containing continuously cropped apples, and enhance apple root vitality, making it suitable for preventing and treating continuous cropping problems.