151 results about "Auxin" patented technology

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

The invention belongs to the technical field of microorganisms, and discloses saline-alkaline tolerant halomonas and application thereof. The Franzmannia qiaohouensis is separated from rhizosphere soil of broom corn millet in saline-alkali soil, the strain number is STM-122, the preservation number is CGMCC No.36349, the classification status is clear through 16S rRNA (ribosomal ribonucleic acid) gene sequence identification and phylogenetic analysis, and the Franzmannia qiaohouensis has the functions of dissolving phosphorus and producing EPS (expandable polystyrene), auxin and siderophore. Through bacterial suspension seed soaking treatment, the germination potential and germination rate of broom corn millet seeds under saline-alkali stress can be remarkably improved, and the indexes of seedling root length, plant height and the like are promoted to be improved; meanwhile, the invention provides a complete preparation method of the bacterial strain fermented bacterial fertilizer, and the viable count of the obtained fermented bacterial fertilizer is greater than or equal to 1 * 10 < 8 > CFU / g. The method can enrich salt-tolerant microbial resources, and provides technical support for planting broom corn millet and other crops in saline-alkali soil.

The invention belongs to the technical field of cultivation and utilization of herba artemisiae annuae, and particularly relates to a method for increasing the breeding success rate of herba artemisiae annuae seeds in alpine regions. The method comprises the following steps: cleaning the fluffy wormwood seeds, then adding the fluffy wormwood seeds into an acid solution, carrying out acid soaking treatment, and then washing the fluffy wormwood seeds to obtain the fluffy wormwood seeds subjected to acid soaking; the method comprises the following steps: adding the acid-soaked artemisia splendens seeds into somatotropin, carrying out growth promoting treatment, washing with water, treating the seeds with alcohol and sodium hypochlorite in sequence, and finally carrying out seed germination treatment. According to the method, through the synergistic effect of the steps, the germination rate of the artemisia splendens seeds can be remarkably increased, the germination time lag is greatly shortened, robust growth of seedlings is promoted, and the problem of large-scale propagation of the artemisia splendens seeds is effectively solved.

The present application belongs to the field of agricultural technology, and particularly relates to a composition for improving salt tolerance of plants and application thereof. The present application applies auxin (in the form of aqueous solution or powder), an aqueous solution of auxin and proline, an aqueous solution of auxin and betaine, an aqueous solution of auxin-proline-betaine ternary complex to plants by foliar spraying or root irrigation. Experiments have proved that auxin can quickly promote the sodium ion efflux of plants, reduce the sodium ion content in plants, and significantly improve the salt tolerance; the above-mentioned complex treatment can produce a synergistic effect, and further improve the growth of plants in a high-salt environment. The composition provided by the present application can be used as a plant salt tolerance promoter in field production, has a remarkable effect on enhancing the adaptability and stress resistance of plants to salt-affected land, has strong practicability, and has good popularization and application value.

The invention discloses a fertilizer for promoting and improving the immunity of plants and a preparation method, and relates to the field of fertilizers. The preparation method comprises the following steps: mixing, smashing and grinding earthworms, eucalyptus leaves, wheat leaves and hawthorn leaves, putting into an ethanol solution, oscillating for 20-24 hours in a shaking table, and centrifugally filtering to obtain an active extracting solution; adding a sodium alginate solution into the suspended bacteria solution and the active extracting solution, and slowly adding carboxymethyl chitosan and a calcium chloride solution; and preparing. According to the invention, the plant growth-promoting rhizobacteria are fixed in the composite hydrogel, and the composite hydrogel can promote colonization of strains at plant root tips and lateral roots so as to regulate deposition of endothelial cell suberite, optimize mineral nutrition balance and abiotic stress response of plants and improve accumulation of auxin at the root tips; meanwhile, an active extracting solution is added into the composite hydrogel, immune response can be activated, and the metabolic activity and antioxidant activity of plants are greatly improved in combination with extracts of other substances.

The invention provides a nutrition regulator based on bioactive substances and a preparation method of the nutrition regulator based on the bioactive substances. Comprising the following raw materials in parts by weight: 10-20 parts of zymosan, 15-25 parts of fulvic acid, 20-30 parts of a high-activity carbon source, 5-10 parts of compound biological enzyme, 8-15 parts of algae auxin, 8-12 parts of compound probiotics, 3-8 parts of betaine, 15-25 parts of compound mineral elements and 5-10 parts of compound trace elements. According to the nutrient regulator, the algae auxin is formed by compounding the undaria pinnatifida fermentation product, the pomegranate peel fermentation product and the enteromorpha fermentation product, the undaria pinnatifida fermentation product promotes diatom growth, the enteromorpha fermentation product promotes green algae growth, the pomegranate peel fermentation product can inhibit growth of harmful algae such as blue-green algae, and the nutrient regulator has a synergistic effect on the algae auxin. Therefore, the proportion of beneficial algae such as diatom is increased and maintained for a long time, stable, efficient and high-quality water color beneficial to culture is formed, and the productivity structure of the pond is fundamentally optimized.

The application discloses an application method of leguminous green manure combined with growth-promoting bacteria for emission reduction and efficiency increase, and relates to the technical field of crop planting. The method comprises the following steps: sowing black bean seeds in soil inoculated with bacillus velezensis, and culturing black bean plants to the full flowering stage, and then crushing and turning over the black bean plants as green manure. A special bacillus velezensis is separated, which has the effects of bacteriostasis, nitrogen fixation, phosphorus dissolution, potassium solution, biofilm formation and auxin production, and has excellent biological characteristics such as stress resistance, rapid growth and easy separation, and the advantages of easy cultivation, safety, harmlessness and no pollution. Meanwhile, the bacillus velezensis can ferment to produce rich metabolic products, has good antibacterial activity and stress resistance. The bacillus velezensis is particularly suitable for being combined with black beans for green manure cultivation, and can maximize the effects of soil fertilization and gas emission reduction of green manure.

The invention discloses an application of an indica rice OsRNU2 gene. The registration number of the nucleotide sequence of the OsRNU2 gene in Genbank is LOC4324161, and the registration number of the nucleotide sequence of the OsRNU2 gene in Genbank is LOC4324161. The difference of the indica rice OsRNU2 gene promoter is the reason for the utilization efficiency and yield of indica rice and japonica rice nitrogen fertilizer, and the indica rice OsRNU2 gene promoter sequence is shown as SEQ ID NO.2. In the background of Huajing indica 74, 159 parts of materials carrying IRAT261 single fragment replacement lines are used for QTL (quantitative trait loci) analysis, and the site qRNU2 influencing the nitrate nitrogen absorption rate is positioned. The OsRNU2 haplotype expression level in indica rice is low, so that the indica rice can accumulate more auxin. The excellent allelic variation of the OsRNU2 in the indica rice is introduced into the japonica rice, so that the nitrogen fertilizer utilization efficiency and yield of the japonica rice can be improved.

The invention discloses application and a method of an iaaM gene in creating a new chrysanthemum flower type, and belongs to the field of plant genetic engineering and ornamental plant molecular breeding. The complete CDS sequence of the iaaM gene derived from agrobacterium tumefaciens is constructed into a plant expression vector and is introduced into a chrysanthemum plant, so that stable overexpression of iaaM in a chrysanthemum body is realized. By improving the level of endogenous auxin of plants, the corolla tube fusion of the ligulate flowers is obviously promoted, the flat petal flower is changed into the key petal flower, and the directional improvement of the flower is realized. Compared with traditional cross breeding, the method has the advantages of being short in breeding period, low in genetic background complexity, controllable in target character and the like, and an effective molecular breeding strategy is provided for precise regulation and control of chrysanthemum flower types.

The application discloses application of a rice OsARF24 gene. The nucleotide sequence of the OsARF24 gene has an accession number of LOC4352211 in Genbank. The rice OsARF24 gene is a key element in an auxin signal pathway. Knocking out the OsARF1 can cause the expression amount of nitrogen metabolism related genes of the rice, the nitrate nitrogen absorption rate, the plant height and the yield of the rice to be reduced, and the nitrogen fertilizer utilization efficiency to be reduced in a comprehensive manner. The function of the OsARF24 gene in positively regulating the nitrogen fertilizer utilization efficiency of the rice is clarified, and a technical route for increasing the nitrogen fertilizer utilization efficiency of the rice by increasing the expression of the OsARF24 in the rice is provided.

The application discloses Zanthoxylum bungeanum miR167b The application belongs to the technical field of genetic engineering, and discloses a gene in the application of regulating plant growth and development miR167b The application constructs an expression vector of the gene, and makes the gene overexpress in tomatoes, and finds that miR167b The overexpression plant is dwarfed, the shape of the fruit is changed, the size and weight of the fruit are significantly lower than those of the wild type, the fruit setting rate is high, the fruit is seedless, the number of fruit chambers is reduced, ovule abortion occurs, and no embryo sac is formed in the interior. Further analysis finds that the indole butyric acid content in the fruit of the overexpression plant is increased, which indicates that miR167b The gene can regulate auxin to induce fruit apomixis, lays a foundation for revealing the apomixis mechanism of Zanthoxylum bungeanum, and provides gene resources and basis for improving the breeding strategy of woody plants, and has important theoretical value and application prospect.

The application discloses a plant-source bio-growth-promoting synergistic fertilizer and a preparation method thereof. The fertilizer is prepared by compounding plant-source composite growth-promoting substances, a composite microbial flora and a carrier material in a specific mass ratio. The plant-source composite growth-promoting substances are prepared by solid-state fermentation and extraction of gynostemma pentaphyllum, astragalus and acanthopanax. The composite microbial flora contains brazilian nitrogen-fixing spirillum, fluorescent pseudomonas and brevibacillus brevis, and is embedded and fixed by using kappa-carrageenan microcapsules containing biochar nanoparticles. The carrier is lignosulfonate-modified nano-attapulgite. The application also provides a corresponding preparation method. The granular fertilizer is finally prepared through steps of activating the strains by a composite activation solution, embedding by microencapsulation and carrier loading granulation. The fertilizer combines the synergistic effect of plant-source active substances and functional microorganisms, significantly improves the activity of the flora and the field colonization capacity, and can effectively promote plant growth, improve crop yield and quality.

The invention discloses a nutrient solution for cultivating cynara plantago, and belongs to the technical field of plant cultivation, the nutrient solution comprises a Hoagland nutrient solution, auxin and a plant growth retardant, the concentration of the auxin is 0-0.01 mg / L, and the concentration of the plant growth retardant is 0.005-0.01 mg / L. According to the method, the problem that the survival rate of the plantain thistle seedlings is low is solved, the overall growth of the plantain thistle seedlings is effectively promoted, the plantain thistle seedlings with developed root systems and robust plant types are efficiently cultivated, and therefore the survival rate of transplanting is remarkably increased. The invention further provides a seedling raising method for cultivating the cynara plantaginis.

This invention belongs to the field of microbiology, specifically disclosing a strain of *Mcrobacterium paraoxydans* and its applications. The *Mcrobacterium paraoxydans* strain TYQ7 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 27216. The applicant discovered that the aforementioned *Mcrobacterium paraoxydans* can secrete auxin, promoting the growth of cucumber seedlings. It also has the ability to interfere with the directional migration of nematodes and kill nematodes, significantly alleviating root-knot nematode stress. Furthermore, it was found to inhibit the normal growth of *Fusarium oxysporum*, and further exhibits the ability to degrade vegetable straw and solubilize phosphorus. Therefore, the applicant believes that the aforementioned *Mcrobacterium paraoxydans* has significant application prospects in the fields of agriculture and environmental protection.

The invention discloses a culture medium for high yield of auxin from trichoderma invading as well as a culture method and application of the culture medium, and belongs to the technical field of microbial fermentation. According to the invention, a special fermentation culture medium and culture conditions for high-yield IAA are designed and optimized aiming at the physiological characteristics of a specific strain Trichoderma invading HD2112, and the problem that efficient synthesis of the strain IAA cannot be realized by the existing general fermentation technology is effectively solved. The core of the technical scheme is that the optimal proportion of glucose, yeast extract powder and specific inorganic salt is determined, and precise pH, temperature and rotating speed parameters are matched, so that strain growth and IAA anabolic flow are synergistically regulated and controlled, the IAA yield in fermentation liquor is increased to 57.32 mg / L which is remarkably higher than that of a control strain, and directional activation of the high-yield potential of the strain is realized.

The present disclosure provides an engineered nucleic acid sequence comprising a gene cassette, the gene cassette comprising one or more regulator genes, the regulator genes including an iadR gene, and at least three catabolic pathway genes downstream from the one or more regulator genes, the at least three catabolic pathway genes including an iadC gene, an iadD gene, and an iadE gene, wherein the engineered nucleic acid sequence is free of an iadA gene and iadB gene. The disclosure also provides bacteria cells comprising the engineered nucleic acid sequence, wherein the bacteria cell is capable of degrading indole-3-acetic acid. Methods for degrading auxin, controlling auxin, and promoting plant growth and root development are provided, comprising providing the bacteria cell and allowing the bacteria cell to interact with roots of a plant.

A microorganism engineered to: produce or overproduce auxin; reduce the production of ethylene; produce or overproduce one or more metal chelator molecules; and / or reduce or knockout the production of

The use of exogenous mRNA isolated from leucoplasts of germinating plants (AEM2-mRNA) to increase directed auxin transport under reduced gravity conditions is described, wherein the mRNA includes sequences encoding proteins of the PIN and AUX1 / LAX transport systems or their regulatory kinases, thereby stabilizing intrinsic cellular polarity and maintaining directed auxin translocation.

The application discloses application of a flavonoid metabolic pathway network key regulation gene GmCHR1 in Arabidopsis thaliana. Research shows that the expression amount of a soybean chalcone reductase (CHR) gene GmCHR1 in soybean roots is significantly up-regulated under low-phosphorus stress, and the low-phosphorus stress enhances the expression of GmCHR1 in the root system of Arabidopsis thaliana, and GmCHR1 influences the response of the root system of Arabidopsis thaliana to low-phosphorus stress. Through construction of a transgenic plant, overexpression of the GmCHR1 gene in Arabidopsis thaliana can promote the growth of Arabidopsis thaliana, significantly improve the biomass, total root length and total phosphorus content of the transgenic Arabidopsis thaliana under normal phosphorus conditions and low-phosphorus conditions; meanwhile, overexpression of GmCHR1 reduces the accumulation of flavonol in the root tip of the transgenic Arabidopsis thaliana, thereby enhancing the accumulation of auxin in the root tip of the transgenic Arabidopsis thaliana, positively regulating the synthesis of auxin in the root system of Arabidopsis thaliana and improving the content of auxin, and playing an important role in improving the growth and phosphorus efficiency of Arabidopsis thaliana.

The application discloses an endophytic fungus with functions of improving stress resistance and promoting growth of Fraxinus mandshurica and application thereof, and belongs to the technical field of microorganisms. Alternaria tenuissima The endophytic fungus is named as Alternaria tenuissima (SLR21), is preserved in the China General Microbiological Culture Collection Center, and has a preservation number of CGMCC NO.42057. The application screens out the endophytic fungus with functions of improving stress resistance and promoting growth of Fraxinus mandshurica by taking Fraxinus mandshurica as a material, provides new microbial resources for mining of new strains of biological energy functions, and provides a new path for solving the problem of difficult forestation of Fraxinus mandshurica. The fermentation liquor of the endophytic fungus contains high contents of polyphenols, polysaccharides, flavones, salicylic acid and auxin and the like, has high drought resistance, salt and alkali resistance and functions of promoting growth of seedling height, can be used as a seed soaking agent alone, and can be applied to promotion of seed germination, and the fermentation liquor of the endophytic fungus can also be used for root irrigation or spraying treatment of seedlings.

The invention provides a hormone inducible ZmEXPB6 promoter and application thereof in construction of a plant genetic transformation system, and belongs to the technical field of genetic engineering. The invention provides a hormone inducible ZmEXPB6 promoter. The nucleotide sequence of the hormone inducible ZmEXPB6 promoter is as shown in SEQ ID NO: 1. According to the embodiment of the invention, after a carrier containing the hormone-induced promoter and a target gene ZmWus2 gene is transferred into a corn immature embryo receptor, induced culture is carried out in a culture medium containing auxin 2 and 4-D. The result shows that the hormone-induced promoter drives the ZmWus2 gene to express, so that not only can a transgenic embryoid be quickly obtained and the conversion rate be increased, but also the yield of the corn immature embryo is increased. And compared with a constitutive promoter 35S promoter, the malformation rate of regenerated seedlings is obviously reduced. The hormone-induced promoter has a wide application prospect in the aspect of constructing a plant genetic transformation system.

The invention belongs to the technical field of plant promoters, and discloses an expression cassette for efficiently indicating the distribution position of auxin in wheat and application of the expression cassette. A DR5V2 promoter fragment induced by auxin IAA is constructed, a beta-glucosidase (GUS) reporter gene is started by the fragment, and the expression fragment is introduced into a monocotyledonous plant high-efficiency expression vector pBK-UBI. On the basis, a plant recombinant expression vector and recombinant bacteria containing a mini 35S promoter and a nuclear localization signal are constructed and are used for promoting expression of a reporter gene in auxin-enriched tissues of plants. After the auxin distribution indication expression box disclosed by the invention is transferred into wheat, the distribution of auxin can be observed by naked eyes through dyeing. The expression cassette can enable the reporter gene to be expressed at the wheat auxin aggregation position, and has important application value in the fields of wheat tillering and spikelet development basic theory research, wheat specific variety auxin distribution verification, wheat new material cultivation and the like.

The invention discloses an immune microelectrode, an immune electrochemical sensor and a preparation method and application of the immune microelectrode and the immune electrochemical sensor. An immune microelectrode prepared by modifying a micron-level metal electrode wire with a carbon-based nano material and a plant hormone antibody is used as a working electrode, and the electrochemical sensor taking the working electrode as a substrate is used for detecting plant hormones in a plant tissue micro-area. A sensor based on the electrode shows good electrode stability in the process of detecting plant hormones in a plant tissue microcell, and the detection range is large. For other interfering substances such as auxin, salicylic acid and the like in a plant body, generated response signals are greatly different from the auxin and the salicylic acid or basically have no response signals, the electrode shows good specificity, and the method for rapidly detecting the plant hormones in the plant tissue microcell through the electrode is convenient and simple. On the basis of plant tissue micro-area detection, in-situ detection can be carried out on plants.

The application belongs to the field of material preparation, and particularly relates to a preparation method and application of CNT@TpBD composite material, wherein CNT@TpBD composite material is synthesized by self-assembling TpBD on the surface of CNT-NH2 through a simple and rapid one-pot method. The obtained composite material has the characteristics of high specific surface area and rich pores (micropores and mesopores). The composite material contains rich delocalized π bonds, amino groups, hydroxyl groups and carboxyl groups, and indole rings, benzene rings, carboxyl groups and amino groups in stress hormones and auxins, so that strong hydrophobic interaction, electrostatic interaction, π-π interaction and hydrogen bonding interaction can be generated between the two, further enhancing the enrichment effect. The adsorption mechanism research shows that the hydrophobic interaction and electrostatic interaction are the main adsorption forces. The composite material is used as an adsorbent of a dispersive solid-phase extraction method, and is used for enrichment and high-sensitivity detection of plant stress hormones and plant auxins.

The invention relates to the technical field of agricultural microorganisms, in particular to a proline microphage GLBv-Z4 with salt-tolerant and growth-promoting functions and application of the proline microphage GLBv-Z4, and the proline microphage GLBv-Z4 is obtained by being separated and screened from beet rhizosphere soil in a comprehensive treatment and utilization trial project test field of saline-alkali cultivated land in Gansu County 2024. The strain has good salt resistance and acid and alkali resistance; the plant growth promoting agent has various plant growth promoting capacities including nitrogen fixation, phosphorus dissolution, potassium dissolution, siderophore production, auxin IAA production and the like. After the strain is inoculated, under salt stress, the salt tolerance of plant seedlings can be remarkably improved, the growth and development of plant roots are promoted, the leaf area and fresh weight of plants are increased, and the accumulation of soluble substances and the activity of an antioxidant enzyme system of the plants can be increased under the salt stress, so that the adverse effect of the salt stress on the plant seedlings is relieved; the method has a considerable application prospect in saline-alkali soil improvement.

The invention discloses an SlFMO1 gene and application thereof in regulation and control of growth and development of tomato plants, and belongs to the technical field of gene engineering. According to the method, a CRISPR / Cas9 gene editing technology is utilized, firstly, a CRISPR / Cas9-gRNA vector is constructed to accurately edit an SlFMO1 gene in tomatoes, GV3101 agrobacterium tumefaciens is utilized to conduct mediated transformation on the tomatoes, and kanamycin serves as a resistance marker to conduct screening to obtain SlFMO1-deleted mutant tomatoes. Phenotype observation and analysis show that after the SlFMO1 gene is knocked out, the plant height and the plant width of a tomato plant are obviously increased, the apical development is enhanced, the stem diameter of the plant is increased, and the inflorescence spacing is increased, which indicates that the SlFMO1 gene negatively regulates the growth and development of the tomato, and meanwhile, the expression of related genes synthesized by auxin, gibberellin and brassinolide is obviously enhanced. The discovery has important theoretical significance and practical application value for follow-up cultivation of ideal tomato varieties.

The present application relates to the technical field of microorganism, in particular to a bacillus SGA510 and application thereof, which is classified and named as Bacillus sp., has been preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO:M20241840.The bacillus SGA510 has good plant growth promoting function and phosphorus solubilizing performance, and can secrete auxin, promote seed germination, promote seedling growth and degrade insoluble phosphorus in soil when used as a single strain in preparation of a compound microbial agent, so that the bacillus SGA510 has wide application prospect and great economic value in the field of biological fertilizer and the like.

The present invention relates to modified bacteria and plasmids (e.g. conjugative plasmids) which are engineered to express auxins and auxin-like molecules (ALMs), to pharmaceutical compositions containing them and their use in the treatment of various metabolic and cardiovascular diseases.

This invention relates to the field of microbial technology and discloses a biocontrol strain for controlling peanut stem rot, wherein the biocontrol strain is *Bacillus pilaris* (…). Paenibacillus peoriae This invention also provides a biocontrol agent containing this strain and its preparation method. Genomic analysis revealed that this strain contains gene clusters encoding active substances such as fusarium and polymyxin, exhibiting significant antagonistic activity against peanut stem rot fungus, and broad-spectrum inhibitory activity against various plant pathogenic fungi, including wheat stem base rot fungus and rapeseed sclerotinia. Furthermore, this strain possesses the ability to secrete auxin, produce proteases, and produce cellulases. The prepared inoculum, applied through root irrigation, can construct a preventative biological barrier in the rhizosphere, providing both disease prevention and growth promotion effects. This invention provides a highly efficient germplasm resource for the green control of crop diseases.