297 results about "Auxin" patented technology

The invention discloses a growth-promoting bacillus velezensis FSUXF-717 capable of tolerating heavy metals and application of the growth-promoting bacillus velezensis FSUXF-717, and belongs to the technical field of agricultural microorganisms. The bacillus velezensis FSUXF-717 has the following advantages: (1) the bacillus velezensis FSUXF-717 has multifunctional metabolic characteristics: various carbon sources such as cane sugar, raffinose and aesculin can be utilized, and the bacillus velezensis FSUXF-717 has the functions of producing long-chain hormone, dissolving phosphorus, secreting ACC deaminase, efficiently forming a biological membrane and the like; (2) broad-spectrum adaptability: the strain has strong tolerance to heavy metal pollution (Cd / Pb / Cr / Ni), drought and broad-spectrum acid-base stress (pH 4.5-9.5); under the condition of relatively low bacterium concentration, the auxin can be stably synthesized in a heavy metal-acid-base composite stress environment. And (3) the activity of soil sucrase and soil phosphatase is enhanced. Excavation of the strain can provide an important germplasm resource basis for development and utilization of novel efficient microbial fertilizers and lay a foundation for green and high-quality development of agriculture.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus mobilis DG-7 and application thereof in inhibiting bolting of angelica sinensis and improving disease resistance of angelica sinensis. The invention provides a strain of bacillus mobilis DG-7, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.31089. The bacillus mobilis DG-7 and the microbial inoculum thereof provided by the invention can play roles in inhibiting early bolting of angelica sinensis and improving disease resistance by increasing the content of plant endogenous melatonin and salicylic acid, reducing the content of abscisic acid, auxin and gibberellin, reducing the content of total phenolic acid and reducing the expression of photoperiod-related genes. In addition, the bacillus mobilis DG-7 does not solubilize phosphorus, so that the promotion effect of high-concentration phosphorus on early bolting is effectively avoided; the laccase activity of the bacillus mobilis DG-7 is high, polyphenol substances can be effectively removed, and the bolting rate can be reduced.

The invention discloses bacillus velezensis capable of tolerating various environmental pollution and promoting growth and application of the bacillus velezensis. A plant endophyte bacillus velezensis L381 strain is identified in wild rice, and the strain is high in environmental adaptability, can grow in the environment with the temperature of 15-40 DEG C and the pH value of 5-8, and can tolerate the NaCl concentration of 4%; the rice growth promoting agent has a good growth promoting function, can secrete auxin IAA, solubilize phosphorus, dissolve potassium and secrete siderophores, and can effectively promote rice growth. Meanwhile, the strain has stress resistance to environmental stress such as heavy metals and antibiotics, and tetracycline can be degraded; the strain has a wide antibacterial spectrum, and can be used as a biocontrol bacterium for preventing and treating various plant diseases. The plant endophyte L381 can adapt to variable temperature conditions and variable soil environments in the natural environment, has very strong practical application potential, not only improves the functions of bacillus velezensis, but also widens the application range of the strain.

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

The invention provides a Kitasatospora xanthophycea JY01 strain and application thereof, the strain is separated from intestinal tracts of spring silkworms, has good disease-resistant and growth-promoting effects, and is specifically expressed in that cellulase, beta-1, 3-glucanase, siderophore and auxin IAA can be secreted; pot experiments show that the biocontrol efficiency of the microbial inoculum to wheat stalk rot is 57.90%, the biocontrol efficiency of the microbial inoculum to corn stalk rot is 46.41%, and the plant height and fresh weight are both significantly increased. The microbial agent is expected to be applied to preparation of biological bacterial fertilizer for effectively preventing and controlling soil-borne fungal diseases.

The invention belongs to the technical field of biological agents, and particularly relates to bacillus siamensis DG-39, a microbial agent and application of the bacillus siamensis DG-39 to inhibition of early bolting of angelica sinensis. According to the bacillus siamensis DG-39 and the microbial inoculum containing the bacillus siamensis DG-39, the physiological and biochemical states of angelica sinensis are effectively changed mainly by increasing the content of endogenous melatonin of angelica sinensis, reducing the content of abscisic acid, auxin and gibberellin and promoting circulation and utilization of nitrogen, and the photoperiod of the angelica sinensis can be effectively improved by reducing expression of related genes of the photoperiod. The effect of inhibiting early bolting of the angelica sinensis is achieved. Meanwhile, the bacillus siamensis DG-39 microbial inoculum has the characteristics of fixing nitrogen, not dissolving phosphorus and producing siderophore, and can promote the absorption and utilization of plants on nitrogen, so that the promotion effect of excessive phosphorus on bolting is avoided; meanwhile, the bacillus siamensis DG-39 laccase is high in activity, can remove phenolamine substances for promoting bolting, and is beneficial to reducing the bolting rate.

Provided is the use of overexpression of the auxin transporter PtoPIN1a gene in increasing the biomass of Populus tomentosa. The nucleotide sequence of the auxin transporter PtoPIN1a gene is as shown in SEQ ID No. 1. By means of using a genetic engineering method, wild-type Populus tomentosa is transformed by means of Agrobacterium tumefaciens infection to obtain a tissue-specific PtoPIN1a overexpressing transgenic plant, and subsequently, a plant with a relatively high expression level is selected, by means of positive identification, for phenotype observation. Results show that compared with the wild type in the same period, the PtoPIN1a transgenic plant has an increased plant height, a thicker stem and a significantly increased aboveground biomass. By means of the provided technical cultivation method, a high-biomass high-quality poplar variety is cultivated. The method is of great significance for future molecular breeding to cultivate excellent poplar varieties.

Herbicidal compositions comprising a herbicidal malonamide (Component A), selected from a compound of formula (I) and an auxin mimic (Component B).

The invention relates to the technical field of microorganisms, and particularly discloses salt-tolerant bacillus XYK4-2 and application thereof. The salt-tolerant bacillus XYK4-2 is preserved in the China General Microbiological Culture Collection Center on May 28, 2024, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC No.30804, and the salt-tolerant bacillus XYK4-2 is classified and named as the salt-tolerant bacillus; the salt-tolerant bacillus XYK4-2 disclosed by the invention has the capabilities of dissolving phosphorus, fixing nitrogen, dissolving potassium, complexing iron and generating auxin, has the capability of generating the auxin (IAA) of 5.71 mg / L, has an effective growth promoting effect on crops such as tomatoes and hot peppers, is wide in application and has certain application potential.

The invention provides a fertilizer synergist for reducing nutrient loss and inhibiting nitrification and application of the fertilizer synergist. The fertilizer synergist is prepared from the following components in percentage by mass: 95 to 98 percent of carboxyl cross-linked modified montmorillonite, 0.05 to 0.1 percent of mannan oligosaccharide, 0.1 to 0.5 percent of mannan, 1 to 3 percent of polyaspartate and 0.5 to 2 percent of a nitrification inhibiting aid. According to the fertilizer synergist capable of reducing nutrient loss and inhibiting nitrification and the application of the fertilizer synergist, the fertilizer synergist serves as a plant nutrient loss control synergist and a nitrification inhibitor and can activate a crop stress resistance signal source, promote and induce synthesis of endogenous auxin in plants, root and growth, activate phosphorus and dissolve potassium, reduce nitrogen loss and improve the yield of the plants. The fertilizer utilization rate is improved.

The invention provides an application of a rice OsCSN5 protein, relates to the technical field of crossing of biotechnology and plant breeding, and particularly provides an application of the rice OsCSN5 protein in a method for controlling the length of a main root of rice and the sensitivity of auxin of a rice root system. The invention further provides application of the rice OsCSN5 gene encoding the rice OsCSN5 protein in a method for controlling the length of a main root of rice and the sensitivity of auxin in a rice root system, the amino acid sequence of the rice OsCSN5 protein is shown as SEQ ID No.2, and the nucleotide sequence of the rice OsCSN5 gene is shown as SEQ ID No.1. The regulation and control effect of the OsCSN5 gene on the growth of the rice root system is analyzed for the first time, and a theoretical foundation is laid for further genetic improvement of rice root system molecules.

The invention provides bacillus subtilis KC-1 as well as a composition and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacillus subtilis KC-1 is CGMCC (China General Microbiological Culture Collection Center) No.35237. The bacillus subtilis KC-1 can secrete IAA, has nitrogen fixation capacity and can generate siderophores. The bacillus subtilis KC-1 and whey protein are used as a composition, and the bacillus subtilis KC-1 and the whey protein jointly act to prepare the seed treatment material, so that a synergistic effect can be achieved, and corn seed germination and seedling root development are remarkably promoted. Experiments show that compared with single use of the whey protein and the bacillus subtilis KC-1, the combined use of the whey protein and the bacillus subtilis KC-1 can significantly improve the seed germination rate, the length of buds on the overground part, the length of main roots on the underground part, the total root volume, the total root surface area, the total root length and the seedling auxin concentration, and also can significantly reduce the concentration of abscisic acid in seedlings, so that the survival rate of the seedlings is improved. The germination of seeds and the growth of seedlings under drought conditions are promoted, and the drought resistance of plants is improved.

The invention belongs to the field of microorganisms, and particularly discloses a Pseudomonas klackmussii strain TYQ5 and application thereof, the Pseudomonas klackmussii strain TYQ5 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.27214, and the Pseudomonas klackmussii strain TYQ5 is preserved in the China General Microbiological Culture Collection Center (CGMCC). The applicant finds that the Pseudomonas kirschner can secrete auxin, can promote the growth of cucumber seedlings, also has the capabilities of interfering tropism migration of nematodes and killing nematodes, has the effect of remarkably relieving stress of root-knot nematodes, also finds that the Pseudomonas kirschner has the capability of inhibiting normal growth of fusarium oxysporum, and also finds that the Pseudomonas kirschner has the capability of inhibiting normal growth of fusarium oxysporum. Furthermore, the pseudomonas kirschner has the capability of degrading vegetable straws and the capability of solubilizing phosphorus, so that the applicant considers that the pseudomonas kirschner has a remarkable application prospect in the fields of agriculture and environmental protection.

The invention belongs to the field of microorganisms, and particularly discloses a microbacterium paraoxidans strain and application thereof, the microbacterium paraoxidans strain TYQ7 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.27216, and the preservation number is CGMCC No.27216. The microbacterium paraoxidans strain TYQ7 has the advantages that the microbacterium paraoxidans strain TYQ7 is preserved in the China General Microbiological Culture Collection Center (CGMCC); the applicant finds that the microbacterium paraoxidans can secrete auxin, can promote growth of cucumber seedlings, also has the capabilities of interfering tropism migration of nematodes and killing nematodes, has the effect of remarkably relieving stress of root-knot nematodes, also finds that the microbacterium paraoxidans has the capability of inhibiting normal growth of fusarium oxysporum, and has the effect of remarkably relieving stress of root-knot nematodes. Furthermore, the microbacterium paraoxidans have the capability of degrading vegetable straws and the capability of dissolving phosphorus, so that the applicant considers that the microbacterium paraoxidans have a remarkable application prospect in the fields of agriculture and environmental protection.

The invention provides pseudomonas chlororaphis, a complex microbial inoculant and application of the complex microbial inoculant in restoration of degraded grassland, and relates to the technical field of microorganisms. The pseudomonas chlororaphis provided by the invention is pseudomonas chlororaphis PS08, and the preservation number of the pseudomonas chlororaphis PS08 is CGMCC (China General Microbiological Culture Collection Center) No.33616. The strain has the capacity of dissolving potassium and phosphorus, also has the capacity of producing siderophores, auxin and ACC deaminase, can improve the seed germination rate, promote plant growth, improve plant biomass and promote plant root development, and is further used for repairing degraded grassland. The complex microbial inoculant prepared from the strain is used for repairing degraded grassland, the soil fertility is improved by constructing a soil structure, nutrient and microorganism multi-dimensional microhabitat and enhancing the density and vitality of underground propagules, and on the basis, alfalfa and wild indigenous grass seeds are reseeded, so that synergistic repairing of vegetation and soil is achieved, and the soil fertility is improved. The above-ground biomass and the pasture quality are improved, and the grassland soil fertility is improved.

The invention discloses a strain of disease-resistant and growth-promoting pseudomonas avenae LC40 and application thereof, and relates to the technical field of microorganisms, the pseudomonas avenae is specifically pseudomonas avenae LC40, the pseudomonas avenae LC40 is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 2, 2024, and the preservation number is CGMCC No.32869. The invention further discloses a method for preparing the disease-resistant and growth-promoting pseudomonas avenae LC40. The bacterial strain can be used for inhibiting tobacco mosaic virus (TMV), tomato brown fruit ruffle virus (ToBRFV), rice blast germ (Magnaporthe oryzae) and alternaria tenuissima (Alternaria tenuissima), and the bacterial strain can be used for inhibiting the tobacco mosaic virus (TMV), the tomato brown fruit ruffle virus (ToBRFV), the rice blast germ (Magnaporthe oryzae (Magnaporthe oryzae (Magnaporthe oryzae (Magnaporthe oryzae) and the allium tenuissima (Alternaria tenuissima). The Pseudomonas Asian LC40 provided by the invention has phosphorus solubilizing capacity and can secrete auxin compounds IAA, and field test results show that the LC40 strain has the effects of promoting growth and increasing yield of tomatoes. The pseudomonas Asian LC40 strain can effectively prevent and control plant diseases and promote crop growth, so that the yield and quality of crops are improved, and the pseudomonas Asian LC40 strain shows good application prospects of disease prevention and growth promotion.

The invention belongs to the field of agricultural microorganisms, and particularly relates to coxakella cocori and application thereof. The coxakia cokei is preserved in the China General Microbiological Culture Collection Center, the classification name of the coxakia cokei is HHQGLZ30-1 (Kosakia cokei HHQGLZ30-1), the preservation date of the coxakia cokei is January 29, 2024, and the preservation number of the coxakia cokei is CGMCC No.29798. The coxakia cokei is named as the coxakia cokei HHQGLZ30-1. The coxakella cocori can secrete auxin, has nitrogen fixation capacity and siderophore production capacity, can convert iron elements which are difficult to absorb and utilize by plants in soil into siderophore chelated iron, and can promote plant growth and antagonize phytopathogens. By applying the growth-promoting and disease-preventing microbial agent prepared from the Corisakia cocori HHQGLZ30-1, the stress resistance of crops can be effectively improved, the growth of the crops is promoted, the crops are healthier, and the leaf surfaces are brighter and greener.

The present invention discloses a method for preparing an acidic soil conditioner from mine waste stone powder, as well as its product and application. The preparation method includes: S1: mixing the mine waste stone powder with an adhesive, granulating and post-treating to obtain stone powder particles; S2: placing waste shells and aquatic product leftovers in an acidic solution, standing for activation, washing to neutrality and then drying for standby; S3: performing high-temperature carbonization and expansion treatment on the product dried in step S2 in an anoxic or oxygen-free environment to obtain biochar; S4: uniformly mixing the stone powder particles prepared in step S1, the biochar prepared in step S3, waste shells, livestock manure, potassium humate and auxin to obtain an acidic soil conditioner. The preparation method disclosed by the present invention uses mine waste stone powder, waste shells and aquatic product leftovers as raw materials, which can not only effectively improve soil problems, solve the problem of waste of waste resources, turn waste into treasure, but also will not cause secondary harm to the soil environment.

The invention relates to the technical field of microbial fermentation, and discloses an agricultural ecological probiotic complex microbial inoculant, which is prepared from the following components in percentage by mass: 30 to 35 weight percent of beneficial microbial inoculant, 20 to 25 weight percent of mineral source potassium fulvate, 15 to 20 weight percent of slow release agent, 10 to 15 weight percent of nano polypeptide fermentation liquor and the balance of carrier, the processing method comprises the following steps: S1, pretreating the raw materials; s2, preparing nano polypeptide fermentation liquor; s3, complex microbial inoculants are compounded; and S4, drying and forming. According to the invention, the mineral source potassium fulvic acid and the nano-polypeptide fermentation liquor are added, so that effective regulation and control of soil micro-ecology are realized; crop growth is promoted through cooperation of multiple strains, growth hormone is secreted through bacillus subtilis and the like, nitrogen is fixed through bacillus amyloliquefaciens, root development is stimulated in cooperation with mineral source potassium fulvic acid, and the plant height, stem diameter and fresh weight of crops are remarkably increased; the particle size of the polypeptide subjected to high-pressure homogenization treatment is 80-120nm, the absorption efficiency is improved by 60%, and a crop disease-resistant signal channel is activated.

The present disclosure provides compositions and methods for altering auxin accumulation in corn or maize plants. Methods and compositions are also provided for altering the expression of genes related to auxin efflux through editing or mutagenesis of a brachytic2 (br2) gene to introduce a premature stop codon or a deletion into the gene such that a truncated Br2 protein encoded by the mutant allele of the br2 gene, which may be a dominant or semi-dominant allele, has at least part of a transmembrane domain without a nucleotide binding domain or motif. Modified plant, plant parts and cells having such a mutant allele with reduced or altered expression or activity of a br2 gene product can have improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types in the plant.

The invention provides a VvYUC10 gene, the nucleotide sequence of the VvYUC10 gene is as shown in SEQ ID No.1, or the nucleotide sequence which is obtained by substituting, deleting and / or adding one or more nucleotides to the nucleotide sequence as shown in SEQ ID No.1 and expresses proteins with the same function; the invention also provides an application thereof. The grape VvYUC10 gene has the advantages that the function of the grape VvYUC10 gene is verified, it is found that the content of auxin in calluses of overexpressed VvYUC10 arabidopsis thaliana and grapes under salt stress is increased, the oxidation resistance is enhanced, and it is further found that the expression quantities of the VvRD22 gene, the VvDREB2A gene, the VvRD29A gene, the VvP5CS gene, the VvCAT1 gene and the VvSOD gene are increased; finally, it shows that the VvYUC10 gene positively regulates and controls the auxin signal channel to positively resist external adverse environmental factor salinization, and a theoretical reference basis is provided for grape salt-tolerant gene mining and breeding.

The invention relates to the technical field of microorganisms, and discloses brevibacillus megatherium, a fungicide containing the brevibacillus megatherium, application of the brevibacillus megatherium and the fungicide and a method for preventing and treating plant diseases. The preservation number of the brevibacillus formmosus is CGMCC (China General Microbiological Culture Collection Center) No.32491, and the brevibacillus formmosus can be used for preparing the bacillus formmosus. The invention also provides a method for preventing and treating plant diseases. The method comprises the step of irrigating plants by using the brevibacillus beautiful and the fungicide. The brevibacillus megaterium has the effects of promoting growth and preventing diseases, has a relatively high bacteriostasis rate on phytophthora nicotianae, can efficiently antagonize black shank caused by phytophthora nicotianae, and has relatively strong capability of secreting auxin and exopolysaccharide.

The invention discloses mortierella alpina GRB-1, a fungicide and application of the fungicide, and belongs to the technical field of agricultural microorganisms. The Mortierella alpina GRB-1 is separated from root systems of healthy fruit trees in a continuous cropping apple orchard for the first time, and the Mortierella alpina GRB-1 has the capabilities of producing protease, producing siderophores, dissolving phosphorus, producing ammonia gas and producing auxin, can inhibit fusarium and has the application potential of relieving continuous cropping obstacles. The product prepared by combining the straw with crop straws has an excellent effect on relieving apple continuous cropping obstacles, can promote the growth of overground parts and underground parts of continuous cropping apple trees, improves the relative abundance of bacterial communities in apple continuous cropping soil and the nutrient content in the apple continuous cropping soil, improves the root activity of apples, and improves the survival rate of the apples. The method can be used for preventing and treating apple successive cropping obstacles.

The invention belongs to the technical field of tobaccos, and particularly relates to a method for improving usability of upper leaves of flue-cured tobaccos. According to the method, after low-nicotine flue-cured tobacco plants are topped, auxin is sprayed to the topped positions and the front faces and the back faces of the tobacco leaves, the low-nicotine tobacco leaves are harvested, then the obtained low-nicotine tobacco leaves are prepared into tobacco shreds, and the tobacco shreds are evenly mixed with high-nicotine upper tobacco shreds to prepare cigarettes. According to the method, the upper tobacco leaves and the tobacco leaves of the low-nicotine flue-cured tobacco variety are mixed and proportioned, so that the taste is balanced, the irritation is reduced, the nicotine content is reduced, the usability of the upper tobacco leaves is improved, and large-scale production of the low-nicotine tobacco leaves is further achieved.

The invention belongs to the technical field of microorganisms, and discloses saline-alkaline tolerant halomonas and application thereof. The Franzmannia qiaohouensis is separated from rhizosphere soil of broom corn millet in saline-alkali soil, the strain number is STM-122, the preservation number is CGMCC No.36349, the classification status is clear through 16S rRNA (ribosomal ribonucleic acid) gene sequence identification and phylogenetic analysis, and the Franzmannia qiaohouensis has the functions of dissolving phosphorus and producing EPS (expandable polystyrene), auxin and siderophore. Through bacterial suspension seed soaking treatment, the germination potential and germination rate of broom corn millet seeds under saline-alkali stress can be remarkably improved, and the indexes of seedling root length, plant height and the like are promoted to be improved; meanwhile, the invention provides a complete preparation method of the bacterial strain fermented bacterial fertilizer, and the viable count of the obtained fermented bacterial fertilizer is greater than or equal to 1 * 10 < 8 > CFU / g. The method can enrich salt-tolerant microbial resources, and provides technical support for planting broom corn millet and other crops in saline-alkali soil.

The invention relates to the technical field of plants, in particular to application of surfactin in promotion of rape root development. According to the invention, a new function of the surfactin is found, and the surfactin can be used as a signal molecule to promote expression of related genes of auxin of the rape and promote development of lateral roots of the rape and formation of a branch prosite, so that development of a root system of the rape is remarkably promoted. Surfactin can be used for preparing products for promoting root development of rape, such as plant growth regulators, fertilizers or fertilizer additives and the like, and has a wide application prospect.

The invention discloses an application of a rice OsARF24 gene. The login number of the nucleotide sequence of the OsARF24 gene in Genbank is LOC4352211, and the login number of the nucleotide sequence of the OsARF24 gene in Genbank is LOC4352211. The rice OsARF24 gene is a key element in an auxin signal path. Knockout of the OsARF1 can cause reduction of the expression quantity, the nitrate nitrogen absorption rate, the plant height and the yield of the nitrogen metabolism related genes of the rice, and the comprehensive performance is that the utilization efficiency of the nitrogen fertilizer is reduced. The function of the OsARF24 gene for positively regulating the utilization efficiency of the rice nitrogen fertilizer is defined, and a technical route for improving the utilization efficiency of the rice nitrogen fertilizer by increasing the expression of the OsARF24 in rice is provided.

The invention relates to a compound microbial fertilizer containing a seaweed extract as well as a preparation method and application of the compound microbial fertilizer, and aims to solve the problems that a traditional embedding material in a microbial fertilizer provided by the prior art is easy to disintegrate in a high-salt environment, a microbial inoculum is easy to inactivate, and the effect of the fertilizer in a high-pH environment is relatively small. Adding a seaweed extract and tannic acid into the bacterial liquid to form microcapsules, taking seaweed oligosaccharide and humic acid composite liquid as an outer slow-release layer, and spraying the bacterial liquid onto the surfaces of fertilizer particles through a nozzle, so that the fertilizer forms a gradient coating structure. According to the preparation method of the compound microbial fertilizer containing the seaweed extract, the fertilizer forms an ion barrier layer in a high-salt environment, natural auxin and humic acid in the seaweed extract synergistically activate strain metabolism, and after the prepared compound microbial fertilizer is applied, the growth effect of crops in a high-pH environment can be greatly improved.

The invention discloses a PagARF16.1 gene for regulating xylem development of poplar and application of the PagARF16.1 gene, and belongs to the technical field of biological genetic engineering. A nucleotide sequence and an amino acid sequence of a coding region of the PagARF16.1 gene are respectively shown as SEQ ID NO. 3 and SEQ ID NO. 4. The PagARF16.1 gene is knocked out by utilizing a CRISPR / Cas9 technology, and an experimental result shows that compared with a wild type poplar, the plant height of the mutant poplar is remarkably reduced, and xylem is remarkably reduced. The result shows that the poplar auxin response factor gene PagARF16.1 is a key regulatory factor for regulating xylem development. The invention provides a new technical means for regulating and controlling the xylem development of the poplar, and has an important forest tree genetic engineering application value.

The invention belongs to the technical field of cultivation and utilization of herba artemisiae annuae, and particularly relates to a method for increasing the breeding success rate of herba artemisiae annuae seeds in alpine regions. The method comprises the following steps: cleaning the fluffy wormwood seeds, then adding the fluffy wormwood seeds into an acid solution, carrying out acid soaking treatment, and then washing the fluffy wormwood seeds to obtain the fluffy wormwood seeds subjected to acid soaking; the method comprises the following steps: adding the acid-soaked artemisia splendens seeds into somatotropin, carrying out growth promoting treatment, washing with water, treating the seeds with alcohol and sodium hypochlorite in sequence, and finally carrying out seed germination treatment. According to the method, through the synergistic effect of the steps, the germination rate of the artemisia splendens seeds can be remarkably increased, the germination time lag is greatly shortened, robust growth of seedlings is promoted, and the problem of large-scale propagation of the artemisia splendens seeds is effectively solved.