Novel strain of streptomyces fradiae and application thereof
A technology of Streptomyces freundii and new strains, applied in the direction of application, bacteria, biochemical equipment and methods, etc., can solve the problems of soil beneficial microbial flora destruction, insufficient supply of organic fertilizer, affecting the quality of agricultural products, etc., and achieve long shelf life , good activation of soil nutrients, and good resistance to various farming obstacles
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Embodiment 1
[0048] Identification of Example 1 Streptomyces flexneri CLIMAX-VSF
[0049] The morphological characteristics of Streptomyces flexneri CLIMAX-VSF of the present invention are shown in the following table.
[0050]
[0051] Physiological and biochemical characteristics are as follows:
[0052] Pilot projects
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Pilot projects
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Pilot projects
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glucose
+
Glycerin
+
sodium malate
+
+
+
sodium gluconate
+
-
+
Sodium citrate
-
-
melezitose
+
sodium succinate
-
+
+
sodium malonate
-
-
+
sodium hippurate
-
Sorbitose
-
+
-
-
+
+
...
Embodiment 2
[0056] The biological characteristic of embodiment 2 Streptomyces flexneri CLIMAX-VSF
[0057] 1. Auxin secretion performance
[0058]1) Activate the freeze-dried tube containing Streptomyces flexneri CLIMAX-VSF first. The specific method is to first insert it into the slant of Gao’s No. 1 medium and cultivate it at 30±2°C for 15 days; after obtaining healthy colonies, insert it into PDA medium and cultivate it at 30°C for 5 days, and then select healthy colonies. The strains were inserted into a sterilized liquid medium consisting of 20 parts of sucrose, 5 parts of sodium chloride, 20 parts of peptone, 5 parts of magnesium sulfate, 3 parts of potassium dihydrogen phosphate, and 1000 parts of water, and then 150 r / Min, cultured at 30±2°C for 3 days to obtain Streptomyces flexneri seed liquid.
[0059] 2) Solid proliferation culture: insert the Streptomyces freundii seed solution into a 120-minute process consisting of 60 parts of wheat bran, 5 parts of brown sugar, 30 parts...
Embodiment 3
[0068] The preparation of embodiment 3 Streptomyces freundii CLIMAX-VSF original bacterial agent
[0069] The present invention uses a liquid-solid combined fermentation culture process to cultivate the Streptomyces freundii inoculum, through the first liquid multiplication culture and then inserting into the solid culture for multiplication and cultivation, the formed inoculum has strong biological enzyme activity, and the specific steps include:
[0070] 1) Seed preparation and liquid multiplication culture: first activate the freeze-dried tube containing Streptomyces flexneri CLIMAX-VSF. The specific method is to first insert it into the slant of Gao’s No. 1 medium and cultivate it at 30±2°C for 15 days; after obtaining healthy colonies, insert it into PDA medium and cultivate it at 30°C for 5 days, and then select healthy colonies. The strains were inserted into a sterilized liquid medium consisting of 20 parts of sucrose, 5 parts of sodium chloride, 20 parts of peptone, ...
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