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Novel strain of streptomyces fradiae and application thereof

A technology of Streptomyces freundii and new strains, applied in the direction of application, bacteria, biochemical equipment and methods, etc., can solve the problems of soil beneficial microbial flora destruction, insufficient supply of organic fertilizer, affecting the quality of agricultural products, etc., and achieve long shelf life , good activation of soil nutrients, and good resistance to various farming obstacles

Active Publication Date: 2012-01-25
BEIJING JINGGENG TIANXIA AGRI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the long-term application of chemical fertilizers, the soil beneficial microbial flora is damaged, the supply of organic fertilizer is insufficient, and the proportion of various nutrients is out of balance, resulting in different degrees of damage to the farmland ecological environment, soil physical and chemical properties and soil microbial flora. The current status of soil resources in my country urgently needs to solve the problem of soil being gradually destroyed through artificial measures to ensure the level of agricultural planting and the quality of crop products.

Method used

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  • Novel strain of streptomyces fradiae and application thereof
  • Novel strain of streptomyces fradiae and application thereof
  • Novel strain of streptomyces fradiae and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Identification of Example 1 Streptomyces flexneri CLIMAX-VSF

[0049] The morphological characteristics of Streptomyces flexneri CLIMAX-VSF of the present invention are shown in the following table.

[0050]

[0051] Physiological and biochemical characteristics are as follows:

[0052] Pilot projects

result

Pilot projects

result

Pilot projects

result

glucose

+

Glycerin

+

sodium malate

+

Mannitol

+

lactose

+

sodium gluconate

+

Salicin

-

Galactose

+

Sodium citrate

-

Raffinose

-

melezitose

+

sodium succinate

-

starch

+

L-arabinose

+

sodium malonate

-

Sorbitol

-

xylose

+

sodium hippurate

-

Sorbitose

-

cellobiose

+

sodium propionate

-

sucrose

-

Erythritol

+

sodium acetate

+

...

Embodiment 2

[0056] The biological characteristic of embodiment 2 Streptomyces flexneri CLIMAX-VSF

[0057] 1. Auxin secretion performance

[0058]1) Activate the freeze-dried tube containing Streptomyces flexneri CLIMAX-VSF first. The specific method is to first insert it into the slant of Gao’s No. 1 medium and cultivate it at 30±2°C for 15 days; after obtaining healthy colonies, insert it into PDA medium and cultivate it at 30°C for 5 days, and then select healthy colonies. The strains were inserted into a sterilized liquid medium consisting of 20 parts of sucrose, 5 parts of sodium chloride, 20 parts of peptone, 5 parts of magnesium sulfate, 3 parts of potassium dihydrogen phosphate, and 1000 parts of water, and then 150 r / Min, cultured at 30±2°C for 3 days to obtain Streptomyces flexneri seed liquid.

[0059] 2) Solid proliferation culture: insert the Streptomyces freundii seed solution into a 120-minute process consisting of 60 parts of wheat bran, 5 parts of brown sugar, 30 parts...

Embodiment 3

[0068] The preparation of embodiment 3 Streptomyces freundii CLIMAX-VSF original bacterial agent

[0069] The present invention uses a liquid-solid combined fermentation culture process to cultivate the Streptomyces freundii inoculum, through the first liquid multiplication culture and then inserting into the solid culture for multiplication and cultivation, the formed inoculum has strong biological enzyme activity, and the specific steps include:

[0070] 1) Seed preparation and liquid multiplication culture: first activate the freeze-dried tube containing Streptomyces flexneri CLIMAX-VSF. The specific method is to first insert it into the slant of Gao’s No. 1 medium and cultivate it at 30±2°C for 15 days; after obtaining healthy colonies, insert it into PDA medium and cultivate it at 30°C for 5 days, and then select healthy colonies. The strains were inserted into a sterilized liquid medium consisting of 20 parts of sucrose, 5 parts of sodium chloride, 20 parts of peptone, ...

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Abstract

The invention discloses a novel strain CLIMAX-VSF of streptomyces fradiae. The preservation number of the novel strain is CGMCC No. 5133; the preservation date is August 12, 2011; and the preservation department is China General Microbiological Culture Collection Center. The invention further discloses a microbial inoculum containing the novel strain. The novel strain CLIMAX-VSF of the streptomyces fradiae, which is provided by the invention, has quite obvious performances of disease resistance, nitrogen fixation, phosphate solubilization and somatotropin secretion. By using the strain, the germination rate and the growth speed in a seedling stage can be obviously improved under the condition that nutrients are not added to soil, and furthermore, diseases in the seedling stage and a growth period are obviously inhibited. On the aspect that the strain is used for the mixed preparation and the synergy of a fertilizer, the strain shows to have the favorable effect of activating soil nutrients and the performance of resisting a plurality of cultivation obstacles.

Description

technical field [0001] The invention belongs to the field of soil fertilizer, and in particular relates to an agricultural microbial preparation and its application. Background technique [0002] White agriculture refers to the industrial new agriculture of microbial resource industrialization, and usually refers to the application of microbial fermentation engineering in agriculture. Agricultural production requires soil to provide nutrients for crop growth and a living environment. The application of chemical fertilizers has played a key role in increasing agricultural incomes for many years. However, due to the long-term application of chemical fertilizers, the soil beneficial microbial flora is damaged, the supply of organic fertilizer is insufficient, and the proportion of various nutrients is out of balance, resulting in different degrees of damage to the farmland ecological environment, soil physical and chemical properties and soil microbial flora. The current statu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/00C12R1/54
CPCY02W30/40
Inventor 刘澎
Owner BEIJING JINGGENG TIANXIA AGRI TECH
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