Umbelopsis nana and applications thereof in preventing leaf spot disease of dendrobium officinale

A technology for Agaric dwarfum and Dendrobium officinale, which is applied in the field of microorganisms, can solve the problems of slow growth rate of Dendrobium officinale, low average yield, occurrence of diseases, etc., and achieves the effects of good development and application prospects, easy preservation, and simple culture conditions.

Active Publication Date: 2014-12-10
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Description
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Problems solved by technology

As wild Dendrobium officinale resources are on the verge of depletion, at present, the market demand of Dendrobium officinale mainly depends on a

Method used

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  • Umbelopsis nana and applications thereof in preventing leaf spot disease of dendrobium officinale
  • Umbelopsis nana and applications thereof in preventing leaf spot disease of dendrobium officinale
  • Umbelopsis nana and applications thereof in preventing leaf spot disease of dendrobium officinale

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Embodiment 1

[0020] Analysis of the effect of Umbelopsis nana JSNL001-3 on the disease resistance of Dendrobium officinale.

[0021] (1) Using cottonseed shell fungus residue as the culture substrate, ammonium nitrate as the exogenous nitrogen source, and sucrose as the exogenous carbon source, prepare a solid culture substrate and put it in an autoclave bag, and put it in a high-pressure condition of 121 ° C and 101.3 kPa Sterilize for 20 minutes. After cooling the culture medium, inoculate the mycorrhizal fungus JSNL001-3 under aseptic conditions, and then carry out artificial culture. The specific culture conditions are 28° C., 12 hours of light + 12 hours of darkness, pH 6.5, and cultured for 25 to 35 days. Process the cultured bacterial agent into 1cm 3 Blocks of large or small sizes are placed on the surface of the cultivation substrate of Dendrobium candidum seedlings. The inoculation amount of solid bacterial agent for each clump of Dendrobium candidum seedlings is 5g. After appl...

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Abstract

The invention discloses umbelopsis nana, the classification and name of the umbelopsis nana are zygomycota mucorales umbelopsis nana, the strain number is JSNL001-3, and the umbelopsis nana is currently collected in China General Microbiological Culture Collection Center (CGMCC) at 15th August, 2014, with the collection number of CGMCC No.9565. The umbelopsis nana JSNL001-3 is remarkable in effect on improving the leaf spot disease during the growth of dendrobium officinale; the umbelopsis nana can be artificially cultured, is fast in breeding speed and convenient for large-scale production, and has excellent development and application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to an agaricus dwarf and its application in dendrobium candidum for resisting leaf spot disease. Background technique [0002] Dendrobium officinale is a perennial epiphytic herb of the genus Dendrobium orchidaceae. Studies have shown that from seed germination to healthy growth of seedlings, there is no need for the participation of symbiotic fungi. As wild Dendrobium officinale resources are on the verge of depletion, at present, the market demand of Dendrobium officinale mainly depends on artificial cultivation, but artificial cultivation of Dendrobium officinale has slow growth rate, low average yield and serious disease occurrence. At present, disease control still mainly relies on pesticides. From the perspective of protecting the ecological environment and food safety, Dendrobium candidum, as a precious traditional Chinese medicine, should reduce the appli...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
Inventor 徐超席刚俊张红岩赵桂华何任红史俊郑凯杨鹤同
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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