Umbelopsis nana and applications thereof in preventing leaf spot disease of dendrobium officinale
A technology for Agaric dwarfum and Dendrobium officinale, which is applied in the field of microorganisms, can solve the problems of slow growth rate of Dendrobium officinale, low average yield, occurrence of diseases, etc., and achieves the effects of good development and application prospects, easy preservation, and simple culture conditions.
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[0020] Analysis of the effect of Umbelopsis nana JSNL001-3 on the disease resistance of Dendrobium officinale.
[0021] (1) Using cottonseed shell fungus residue as the culture substrate, ammonium nitrate as the exogenous nitrogen source, and sucrose as the exogenous carbon source, prepare a solid culture substrate and put it in an autoclave bag, and put it in a high-pressure condition of 121 ° C and 101.3 kPa Sterilize for 20 minutes. After cooling the culture medium, inoculate the mycorrhizal fungus JSNL001-3 under aseptic conditions, and then carry out artificial culture. The specific culture conditions are 28° C., 12 hours of light + 12 hours of darkness, pH 6.5, and cultured for 25 to 35 days. Process the cultured bacterial agent into 1cm 3 Blocks of large or small sizes are placed on the surface of the cultivation substrate of Dendrobium candidum seedlings. The inoculation amount of solid bacterial agent for each clump of Dendrobium candidum seedlings is 5g. After appl...
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