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305 results about "Disease control" patented technology

Tartary buckwheat pest and disease damage dynamic monitoring method, system, equipment and medium

The invention relates to a tartary buckwheat pest and disease damage dynamic monitoring method, system and device and a medium, and belongs to the technical field of agricultural intelligent monitoring, the dynamic monitoring method comprises the following steps: periodically collecting environmental parameter data through fixed sensor nodes deployed in a farmland, and obtaining leaf vibration signals and multispectral image data at the same time; performing space-time alignment on the blade vibration signal and the multispectral image data, correcting radiation distortion in the multispectral image data, and outputting a registration data set; according to the registration data set, fusing to generate a multi-modal feature vector, inputting the multi-modal feature vector into a pre-trained space-time analysis model, and outputting a risk level distribution diagram with a geographic coordinate mark; generating a control instruction set according to the risk level distribution map, and triggering execution equipment to execute pest control operation; and optimizing weight parameters of the space-time analysis model through the generative adversarial network based on the execution log of the control instruction set and the historical multi-modal feature vector. The scientificity and timeliness of pest control can be improved.
Owner:LIANGSHAN YI AUTONOMOUS PREFECTURE ACAD OF AGRI SCI

Semantic segmentation and grading method for cross-scale identification of rice black dwarf disease and lodging

The invention relates to the field of disease control, and discloses a semantic segmentation and grading method for cross-scale identification of rice black dwarf disease and lodging, and the method comprises the following steps: S1, collecting rice field data under different elevation scales, and processing the data through software to obtain image data; s2, based on the altitude information of the DSM, determining a classification threshold by combining elevation error correction and height distribution difference statistical analysis, and constructing a DSM-guided auxiliary supervision mask; s3, constructing a cross-scale semantic fusion network, and inputting the image data set in S1 and the auxiliary supervision mask in S2 into the network for training to obtain a rice black dwarf disease and lodging detection model; and S4, predicting by using the rice black dwarf disease and lodging detection model in the S3. By constructing a digital surface model and a digital orthoimage with aligned pixels and combining elevation error correction, accurate association of rice field data under multiple elevation scales can be realized, and support is provided for rice black dwarf disease and lodging identification.
Owner:ZHEJIANG UNIV

Multi-modal data driven farming decision-making system based on large model

The invention provides a multi-modal data driven farming decision-making system based on a large model. According to the system, the accuracy of farming decision making is improved through remote sensing images and field meteorological sensor data. Through integration of multi-modal data, real-time monitoring and accurate decision support of farming operation are realized, and the method aims at overcoming deviation of single-modal data decision and ensuring crop growth and yield. The specific construction process comprises the following stages: 1, collecting crop and farming historical data; 2, processing data, and constructing an agricultural database; thirdly, finely adjusting the multi-modal large model, setting cue words and optimizing the model; 4, processing daily data by using the fine-tuned model, and generating irrigation, fertilization and pest control decisions; fifthly, when the system detects early warning information, a user is notified in time; and 6, carrying out data analysis and reporting, and researching the crop growth trend and environment change. According to the method, multi-modal data are fully utilized, and powerful support is provided for accurate farming decision making.
Owner:XINJIANG UNIVERSITY

Bacillus thuringiensis CMS-BT17 and application thereof

PendingCN121427774ABiocideMicroorganismsAureobasidium sp.Microbiological culture
The invention discloses a bacillus thuringiensis CMS-BT17, which is classified and named as bacillus thuringiensis, is preserved in the China General Microbiological Culture Collection Center (CGMCC), has the preservation number of CGMCC 35313, and is preserved on July 21, 2025. The bacillus thuringiensis strain CMS-BT17 directly separated and screened from a zizania aquatica soil sample in Haikou City of Hainan carries four insecticidal protein coding genes of Cry8Ca, Cry8Da, Spp1Aa and Vpb4Ca, not only is high in insecticidal toxicity and good in insecticidal effect, but also has a certain effect of inhibiting tomato fungal diseases, can be widely applied to prevention and control of agricultural diseases and pests, and has a wide application prospect. The sustainable development of green agricultural production is promoted.
Owner:VEGETABLE RES INST OF HAINAN ACAD OF AGRI SCI

Stress-tolerant bacillus velezensis L-29 and application thereof

The invention relates to the technical field of agricultural microorganisms, and discloses stress-tolerant bacillus velezensis L-29 and application thereof. The bacillus velezensis L-29 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.35587. The bacillus velezensis L-29 is named as bacillus velezensis L-29. Experiments prove that the bacillus velezensis L-29 can adapt to various extreme environments, can survive and proliferate under the extreme environments of drought conditions simulated by 0-25% PEG 6000 under the conditions that the pH is 2-12 and the salt concentration is 1.5%, and has various functions of soil improvement (organic matter decomposition and phosphorus activation), plant growth promotion, broad-spectrum disease control and the like. In the secondary fermentation stage of compost, humus generation can be remarkably promoted, compost maturation is accelerated, the good colonization ability is achieved, and after an obtained compost product is applied to normal soil, the nutrient effectiveness can be improved, crop growth is promoted, and the disease resistance is enhanced.
Owner:CHINA AGRI UNIV +1

Application of Ustilaginoidea virens UvWSC1 gene in improvement of plant disease resistance

The invention provides an application of a Ustilaginoidea virens UvWSC1 gene in improvement of plant disease resistance. The invention develops a new application of the Ustilaginoidea virens UvWSC1 gene in improving the broad-spectrum disease resistance of crops such as rice for the first time, and the UvWSC1 gene is subjected to heterologous high expression in the rice, so that the broad-spectrum resistance of the rice to various pathogenic bacteria such as Ustilaginoidea virens, Magnaporthe oryzae and Xanthomonas oryzae can be obviously enhanced, and the agronomic traits of the rice are not influenced; an important theoretical basis is provided for rice disease-resistant breeding. By heterologous expression of the UvWSC1 gene, broad-spectrum disease resistance can be safely and effectively endowed to crops, a broad-spectrum disease-resistant rice variety is obtained for production, and the broad-spectrum disease-resistant rice gene has great application prospects and economic values in the aspects of crop breeding disease resistance improvement, rice disease prevention and control and crop yield increase and pesticide reduction.
Owner:ANHUI AGRICULTURAL UNIVERSITY

Bacillus amyloliquefaciens QGFCH3-3 as well as fungicide and application thereof

The invention belongs to the technical field of microorganisms, and particularly discloses bacillus amyloliquefaciens QGFCH3-3 as well as a microbial agent and application of the bacillus amyloliquefaciens QGFCH3-3. The preservation number of the bacillus amyloliquefaciens QGFCH3-3 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No.34814 The strain has the functions of nitrogen fixation, amylase production, ligninase production, protease production and cellulase production, can effectively promote plant growth, has broad-spectrum antibacterial activity, can significantly inhibit various pathogenic bacteria, and significantly improves the disease control effect of crops.
Owner:NANNING HARWORLD BIOLOGICAL TECH CORP +1

Intelligent tuberculosis screening and data analysis management system

The invention relates to the technical field of medical artificial intelligence and public health data management crossing, in particular to an intelligent tuberculosis screening and data analysis management system which comprises a data acquisition module, an intelligent screening module, a data analysis management module, a clinical interaction module and a system management module. The multi-source data fusion and the dynamic threshold reasoning enable the early-stage tiny focus detection rate to be larger than or equal to 85%, the screening time is shortened to 5 minutes per case (80% higher than that of manual film reading), cross-mechanism and cross-dimension data integrated acquisition and sharing are achieved, 'hospital-disease control-grassroots' data intercommunication is achieved, and the screening efficiency is improved. Complete data support is provided for epidemiological analysis, longitudinal individual tracking and transverse group analysis are combined, the early warning period of regional outbreak is larger than or equal to 2 weeks, and a public health prevention and control gateway is assisted to move forwards.
Owner:丽水市中医院

Compound fertilizer with nitrogen fertilizer slow-release and biocontrol functions and preparation method thereof

The application discloses a kind of compound fertilizer with nitrogen fertilizer slow-release and biocontrol function and preparation method, it is related to microbial compound fertilizer field, including the following components: nitrogen source carrier, physical net trapping material, 3,4-dimethyl pyrazole phosphate, biocontrol component, binder.A kind of preparation method, including the following steps: after silanization modification treatment is carried out to attapulgite, with polyglutamic acid aqueous solution mixed reaction, obtain self-assembly gel;Bacillus subtilis is embedded and handled, rhamnolipid is pre-emulsified, chitosan oligosaccharide is crosslinked with boric acid treatment, and is blended;Nitrogen source carrier is heated to melt, add self-assembly gel, mix evenly;Temperature control, add 3,4-dimethyl pyrazole phosphate, and keep warm reaction;Cooling, add the blend obtained in step S200, binder, mix evenly;Extrusion granulation, after fluidized bed drying, slow-release coating is carried out, and the target compound fertilizer is obtained.The application makes disease control efficiency to improve to 89.2%, and blocks nitrosomonas AMO enzyme activity for 120 days.
Owner:SICHUAN RUIXIANG AGRI TECH DEV CO LTD

Use of two indole compounds as colletotrichum gloeosporioides inhibitors

The application relates to the field of agricultural disease control technology, and particularly relates to the use of indole compounds and pharmaceutically acceptable salts, solvates, crystal forms, prodrugs, stereoisomers and tautomers thereof for preparing a medicine for inhibiting pathogenic key protein kinase CgSlt2 of pathogenic fungi. The application first proposes a new type of CgSlt2-targeted inhibitor, specifically, indole compounds ((S)-N-(1-(1H-indol-3-yl)-4-(methylamino)-4-oxobutan-2-yl)-8-bromo-1,6-naphthyridine-2-carboxamide) and ((S)-N-(2-((1H-indol-3-yl)-1-(methylamino)-1-oxopropyl)-8-bromo-1,6-naphthyridine-2-carboxamide) are used as CgSlt2 inhibitors, have the characteristics of high target specificity and high activity, and exhibit excellent pathogenicity inhibition of a strain of the complex group of Colletotrichum gloeosporioides.
Owner:CHINA AGRI UNIV SANYA RES INST

Heritable immunization for disease control

PCT designated stageWO2026136419A1Antibacterial agentsHydrolasesDiseaseMicrobiology
In certain aspects, the invention provides methods and compositions for heritable immunization and their use in infectious disease control. The invention also provides methods of preparing organisms and model organisms that can heritably transfer immunization to offspring.
Owner:MASSACHUSETTS INST OF TECH

Protein capable of inducing plant immune activation function and application of protein in disease control

The invention discloses a protein capable of inducing a plant immune activation function and application of the protein in disease control, the gene sequence of the protein capable of inducing the plant immune activation function is shown as SEQ ID NO: 1, and the amino acid sequence of the protein is shown as SEQ ID NO: 2. According to the invention, a novel plant immune activator protein CgSsp16 secreted by colletotrichum gloeosporioides is identified, can be identified by plants, stimulates a plant immune response mechanism, triggers broad-spectrum resistance of host plants to various pathogens and improves disease resistance of various plants; the compound is expected to be developed into a novel biopesticide to be applied to green and sustainable production of agriculture.
Owner:NANJING FORESTRY UNIV

Ancient city wall daily management and disease management and control system and method based on BIM scene construction

The invention belongs to the technical field of cultural relic protection and building information models, and discloses an ancient city wall daily management and disease management and control system and method based on BIM scene construction. An existing disease management and control system has the problems that the urban wall occurrence environment and the repair history are difficult to comprehensively consider, and multi-disease collaborative analysis is lacked. According to the method, non-structural nodes are introduced, environmental factors such as a drainage system, vegetation coverage, underground facilities and an internal space are incorporated into a city wall management system, and collaborative expression of a city wall structure and an occurrence environment is realized on the basis of structural partitioning. According to the system, urban wall block numbers serve as a unified index, monitoring data, disease information and repair records are fused, spatial visualization and information linkage are achieved based on a BIM scene, meanwhile, a threshold value management mechanism is set, when state parameters of non-structural nodes reach a threshold value, a corresponding maintenance protection scheme is triggered, and the maintenance and protection efficiency is improved. And urban wall information management, disease dynamic monitoring and scientific repair decision making are realized.
Owner:XIAN CONSTR SCI & TECH UNIV ENG TECH CO LTD

Inflammation-related prediction model aiming at curative effect and prognosis of advanced gastric or gastroesophageal junction adenocarcinoma first-line chemotherapy combined with immunotherapy and application thereof

PendingCN121641468AMedical simulationHealth-index calculationDiseaseRemission rate
The invention discloses a risk score prediction curve of objective remission rate (ORR), disease control rate (DCR), progression-free lifetime (PFS) and total lifetime (OS) of a patient with advanced gastric or gastroesophageal junction adenocarcinoma after first-line chemotherapy and immunotherapy based on clinical inflammation indexes and application of the risk score prediction curve. The risk score is established based on clinical inflammation indexes and used for drawing a prediction curve of PFS and OS after first-line chemotherapy and immunotherapy and ORR and DCR, common inflammation-related laboratory indexes are integrated, the calculation method is simple, and clinical doctors and patients can conveniently operate to predict ORR, DCR, PFS and OS after treatment. Meanwhile, the risk score prediction model can provide certain help for selection of treatment schemes of later gastric or gastroesophageal junction adenocarcinoma patients in the future.
Owner:邱妙珍 +2

Evaluation system and method for psoriasis control state

PendingCN121662249AMedical data miningMedical automated diagnosisPsoriasis patientDisease
The invention discloses a disease control state evaluation system and method based on psoriasis patient feedback, subjective feelings of patients on disease control can be captured in clinical and non-clinical scenes, and a concept framework is based on literature and doctor-patient feedback. Different from the past psoriasis PROM, the invention provides a method for comprehensively, standardly and quickly collecting'disease control 'information, and the structure content has value for clinical and scientific research. Statistical analysis shows that good clinical benefits can be obtained when the model is applied clinically, and an effective basis for individualized precise treatment is provided for improvement and self-management of psoriasis, especially chronic diseases in China.
Owner:XIANGYA HOSPITAL CENT SOUTH UNIV

Vibrio binding protein of cynoglossus semilaevis and application thereof

ActiveCN116102636BBiotechnologyDisease
This invention provides the application of Vibrio receptor protein type VI collagen α2 from the tongue sole as a target protein for blocking Vibrio pathogen adhesion and infection of tongue sole cells, providing an important foundation for the research and development of strategies and disease control technologies for the prevention and treatment of vibrio infections in tongue sole. This invention discovers that tongue sole type VI collagen α2 can mediate the binding of various Vibrio pathogens to tongue sole, and can serve as a candidate protein for blocking Vibrio adhesion and infection of tongue sole cells, thus providing a new strategy for the prevention and treatment of vibrio diseases in tongue sole. This invention also provides a new approach for the development of novel antibacterial drugs, which has application value in the prevention and treatment of tongue sole diseases.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI

Caribike Yarrowia spp. As well as separation and screening method, identification method and application thereof

PendingCN121294168ABiocideFungiMeyerozyma caribbicaFragaria
The embodiment of the invention discloses yarrowia caribeckii as well as a separation and screening method, an identification method and application of the yarrowia caribeckii. The preservation number of the yarrowia caribica is CGMCC (China General Microbiological Culture Collection Center) No.27604, and the preservation number of the yarrowia caribica is CGMCC No.27604. The Caribik Yarrowia spp. Provided by the embodiment of the invention has a good antagonistic effect on gray mold, penicilliosis, black spot and aspergillus niger after fruits are picked, and can obviously reduce the disease morbidity of the fruits of strawberries, apples, tomatoes and pears. And the antagonistic bacterium is identified to be Meyerozyma caribbica, and the antagonistic bacterium can be used as the Meyerozyma caribbica. By respectively adding auxiliary factors, namely calcium chloride, alginate oligosaccharide (AOS), phytic acid (PA) and metschhia pastoris, the disease prevention and control effects of Meyerozyma caribbica can be enhanced. The disease prevention and control effects of the Meyerozyma caribbica can be enhanced by adding the auxiliary factors of calcium chloride, alginate oligosaccharide (AOS), phytic acid (PA) and metschhia pastoris.
Owner:SHANXI AGRI UNIV

Tobacco leaf surface fertilizer for improving oil content and anti-epidemic ability of tobacco leaves and preparation method thereof

The present application belongs to the technical field of tobacco leaf fertilizer, and provides a tobacco leaf fertilizer for improving oil content and anti-epidemic ability of tobacco leaves, which comprises water and active ingredients, wherein the active ingredients comprise the following components in parts by weight: 240-300 parts of sodium humate, 40-60 parts of potassium nitrate, 15-30 parts of magnesium sulfate heptahydrate, 1-2 parts of striga lactone, 5-8 parts of N-succinylated chitosan, and 1000-1400 parts of tobacco stem enzymolysis fermentation product. The leaf fertilizer can significantly improve the oil content of tobacco leaves, and the required content of active ingredients is low when the leaf fertilizer is applied, thereby reducing the application cost. Meanwhile, the leaf fertilizer can also significantly improve the disease control ability of tobacco, and has good popularization and application value.
Owner:KUNMING UNIVERSITY

Wrapped slow-release chlorine composition as well as preparation method and application thereof

InactiveCN121511975ABiocideSpecific water treatment objectivesMicrosphereChitosan microsphere
The invention belongs to the technical field of sterilization and disinfection materials, and particularly relates to a wrapped slow-release chlorine composition and a preparation method and application thereof.The composition is of a unique dual-wrapping structure, the core is a mixture of sodium chlorite and a stabilizer, the composition is primarily wrapped with hydrophobic modified chitosan microspheres and then combined with citric acid, and the wrapped slow-release chlorine composition is prepared. And finally, the metal coordinated polyethylene glycol hydrogel is used for outer layer coating to form a complete slow-release system. The preparation method comprises the following steps: firstly, preparing a microsphere carrier through hydrophobic modification and cross-linking curing of chitosan and lauric aldehyde; then, polyethylene glycol amine and a derivative of pyridinedicarbonyl chloride are subjected to cobalt ion coordination to form hydrogel; finally, the chlorine source components are combined and formed through adsorption, mixing and coating processes in sequence. The composition can effectively control the release rate of the chlorine component, has long-acting and stable sterilization and disinfection effects, and can be widely applied to the fields of water treatment, food preservation, medical instrument disinfection, agricultural disease control and the like.
Owner:SHENZHEN YUELAI YUEHAO NEW RETAIL TECH CO LTD

A bactericidal composition containing WML-01 and a triazole compound, preparation and application thereof

This invention discloses a fungicide composition containing WML-01 and triazole compounds. The fungicide composition is characterized by comprising WML-01 and triazole compounds in a mass ratio of 10:1 to 1:10. The fungicide composition provided by this invention, with WML-01 and triazole agents as active ingredients, exhibits a synergistic effect in the control of rice bakanae disease, wheat stem rot, wheat scab, plant root rot, and rapeseed sclerotinia stem rot. It can effectively improve disease control efficacy, reduce pesticide usage, delay the development of pesticide resistance, and increase crop yield. It is safe, effective, and economical, and has broad market application prospects.
Owner:CHINA AGRI UNIV SANYA RES INST +1

Apparatus, system and method for adaptive emission of radiation in interior environments

A germicidal disinfection apparatus, system and method. Embodiments of the present disclosure include an adaptive germicidal disinfection apparatus, system and method comprising one or more sensors communicably engaged with a controller being configured to control the operation of at least one UV-C emitter. The sensors may be configured to detect various signals from an interior environment and communicate such signals as inputs to the controller. The controller may be configured to process the inputs according to one or more data processing framework to assess and determine a variety of disease control risk factors and trigger conditions. The controller may be configured to configure one or more modes of operation for the at least one UV-C emitter according to an output of one or more data processing operations.
Owner:UD INNOVATIONS LLC

Method for preventing and treating sclerotinia rot of colza by antagonistic trichoderma strains

The invention discloses a method for preventing and treating sclerotinia rot of colza by antagonistic trichoderma strains, and relates to the technical field of agricultural disease prevention and control, and the method comprises the following specific steps: S100, strain screening and mutagenesis: separating trichoderma strains from rhizosphere soil of colza in different environments, primarily screening strains with antagonistic action on sclerotinia sclerotiorum by a plate confrontation method, and carrying out mutagenesis on the strains with antagonistic action on sclerotinia sclerotiorum; carrying out chemical mutagenesis treatment on the primarily screened strains, carrying out confrontation culture on the primarily screened strains and sclerotinia sclerotiorum again, screening out high-yield antagonistic substances with larger inhibition zones and mutant strains with strong stress resistance, and culturing to obtain trichoderma fermentation liquor; by obtaining the trichoderma strains which can keep efficient antagonism and strong stress resistance under different environmental conditions, the stability of the trichoderma strains on disease control is enhanced, so that the rape can effectively resist invasion of sclerotiniose under different environmental conditions in the growth process, and the disease resistance of the rape is improved. And antagonistic active substances are extracted to prepare a biological fertilizer carrier, and the biological fertilizer carrier is applied to the planting process of oilseed rape, so that the growth and reproduction of sclerotinia sclerotiorum are effectively inhibited.
Owner:INST OF PLANT PROTECTION JIANGXI ACAD OF AGRI SCI

Primer and kit for rapidly detecting yersinia rouxii through RPA-CRISPR / Cas12a and application

The invention discloses a primer and a kit for rapidly detecting yersinia rouxii through RPA-CRISPR / Cas12a and application of the primer and the kit, and relates to the technical field of disease prevention and control in aquaculture. The invention aims to solve the problems that the existing detection method is time-consuming and labor-consuming, is easy to cause pollution and needs to be matched with professional detection equipment. According to the present invention, the RPA amplification primer is composed of a forward primer yrp1-RPA-F2 and a reverse primer yrp1-RPA-R2, the crRNA is yrp1-crRNA-R1, and the kit for rapid detection of yersinia rouxii contains the RPA amplification primer and the crRNA. The method for rapidly detecting yersinia rouxii comprises a fluorescence method and a test paper method. The method disclosed by the invention has the advantages of good inclusiveness, strong specificity, high sensitivity and the like, detection is carried out based on the RPA primer and the crRNA, and the obtained RPA product is detected by using a lateral flow chromatography test strip and fluorescence, so that the purpose of portable visual rapid detection on site or in a breeding base is achieved, and the method is suitable for large-scale popularization and application. The method has very high clinical application value in the aspects of early discovery of diseases, epidemiological investigation and comprehensive prevention and control.
Owner:HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI

Rice growth state perception and farming decision method based on multi-modal semantic guidance

This invention relates to a method for rice growth status perception and agricultural decision-making based on multimodal semantic guidance, belonging to the fields of smart agriculture, computer vision, and artificial intelligence. The method includes acquiring rice canopy images; constructing a dataset; utilizing a small number of samples labeled by agricultural experts and their corresponding descriptive semantic information; introducing a multimodal semantic-guided auxiliary labeling mechanism to generate growth status labels for the remaining samples, thereby reducing data labeling costs and improving labeling consistency; based on the labeled dataset, constructing a multi-task recognition model to collaboratively identify multi-dimensional growth states of rice, such as phenological stage, nutritional status, disease type, disease impact degree, and population growth, within a unified feature space. Based on the multi-task recognition results, rice growth status analysis information is generated, forming agricultural guidance strategies such as fertilization management and pest and disease control, which are then distributed to field execution equipment, realizing an intelligent management closed loop combining rice growth status perception and agricultural decision-making.
Owner:CHONGQING UNIV OF POSTS & TELECOMM

Application of kushenol F in preparation of antibacterial drugs

PendingCN121488962ABiocideFungicidesNicotiana tabacumAlternaria tenuissima
The invention relates to the technical field of medicinal botanical bactericides, in particular to application of kushenol F in preparation of antibacterial drugs. The sophora alcohol F is found to be used for inhibiting the growth of phytophthora nicotianae and alternaria tenuissima. Compared with the traditional chemically synthesized antibacterial agent, the compound has lower toxicity and lower potential risk to the environment and human health, and provides a new choice for disease control in agricultural production.
Owner:YUNNAN UNIVERSITY OF CHINESE MEDICINE +1

Method for preparing biocontrol engineering bacteria by using trichoderma harzianum as dsrna carrier

ActiveCN116286937BBiocideFungiGene technologyPathogenicity
This invention belongs to the field of gene technology for disease control. This invention provides a method for preparing biocontrol engineered bacteria using *Trichoderma harzianum* as a dsRNA vector, comprising the following steps: determining the target gene based on the pathogenic fungus; using the target gene as an arm sequence, and centering on the VdTublin intron sequence of the *Verticillium dahliae* endogenous gene, connecting two identical arm sequences to both ends of the intron in a forward and reverse direction, respectively, to construct the target sequence; and transferring the target sequence into *Trichoderma harzianum* using ATMT to obtain engineered *Trichoderma harzianum* bacteria. The engineered strain obtained by this invention has a good inhibitory effect on the growth and pathogenicity of the corresponding fungus.
Owner:INST OF MICROBIOLOGY CHINESE ACAD OF SCI

Method for improving survival rate of forsythia tender branch cutting seedling

This invention belongs to the field of plant seedling technology, specifically relating to a method for improving the survival rate of Forsythia suspensa cuttings. Specifically, fruiting mother trees are selected, and semi-lignified branches of the current year are collected as cuttings, retaining plump buds. The cuttings are soaked in a rooting agent compound solution and then dried for later use. The treated cuttings are then inserted into a gradient substrate and thoroughly watered after planting. The stomatal conductance of the cutting leaves and the electrical conductivity at the base of the cuttings are monitored in real time. The substrate temperature, spraying frequency, shading rate, and ventilation are adaptively adjusted in a closed-loop manner according to the physiological thresholds of different rooting stages of Forsythia suspensa cuttings. Stress-resistant fertilizer is applied according to the physiological state of the cuttings, and disease control and daily management are carried out. This method improves the survival rate of Forsythia suspensa cuttings, reduces the rate of cutting rot, exhibits strong environmental adaptability, and high seedling stability.
Owner:LONGNAN HONGFENG AGRICULTURAL SCIENCE & TECHNOLOGY CO LTD

Preparation method of sea cucumber-derived multifunctional active peptide Aj-UBL

PendingCN122278891ABiotechnologyProtozoa
This invention discloses a method for preparing the multifunctional bioactive peptide Aj-UBL from sea cucumber, relating to the fields of biotechnology and biomedicine. Using the Aj-UBL coding sequence from sea cucumber as a template, the method involves PCR amplification, recombinant expression vector construction, engineered bacterial induction expression, bacterial cell disruption, affinity chromatography purification, and identification to obtain the target peptide possessing triple activities of antibacterial, antiprotozoal, and antitumor activity. This invention employs an optimized coupling process of genetic engineering and affinity chromatography to achieve high-purity, high-quality, and stable product preparation, while being environmentally friendly and free from drug resistance risks. It not only solves the problem of coordinated disease control in sea cucumber farming but also expands the high-value application of sea cucumber resources in the biomedical field, filling the technological gap in the large-scale preparation of multifunctional bioactive peptides from sea cucumber and the biological control of ciliates, possessing significant theoretical and practical application value.
Owner:LIAONING ACAD OF MARINE FISHERIES SCI (DALIAN INST OF BIOTECHNOLOGY LIAONING ACAD OF AGRI SCI LIAONING MARINE ENVIRONMENT MONITORING STATION)

Complex microbial inoculant and application thereof in prevention and control of tobacco black shank and tomato spotted wilt

The invention relates to a complex microbial inoculant and application thereof in prevention and control of tobacco black shank and tomato spotted wilt, and belongs to the technical field of prevention and control of tobacco diseases. The complex microbial inoculant comprises antibacterial lipopeptide and bacillus subtilis, wherein the mass ratio of the antibacterial lipopeptide to the bacillus subtilis is (0.5-1.2): 10; 0.08 wt% to 0.12 wt% of antibacterial lipopeptide powder is adopted as the antibacterial lipopeptide; the antibacterial lipopeptide comprises at least one of surfactin, iturin and abinogen; the viable count of the bacillus subtilis is greater than or equal to 2 * 10 < 9 > CFU / g. According to the invention, a combined prevention and control method taking antibacterial lipopeptide and bacillus subtilis as cores is adopted, so that the synergistic prevention and control of tobacco black shank and tomato spotted wilt are realized, and meanwhile, the agricultural and economic characters of tobacco are improved; due to the dual effects of the bacillus subtilis and the antibacterial lipopeptide, the lasting period can be prolonged, and the stability is high; the complex microbial inoculant can be completely biodegraded and is suitable for organic agriculture.
Owner:YUNNAN TOBACCO CORP QUJING BRANCH

Specific primer for detecting root rot of wild bluegrass, rapid detection method and application thereof

PendingCN122357768ANucleotideGenetics
This application provides specific primers, rapid detection methods, and applications for detecting root rot of *Imperata cylindrica*. The primer pairs for detecting or assisting in the detection of root rot of *Imperata cylindrica* include primer Rd-F and primer Rd-R. The nucleotide sequence of primer Rd-F is shown in SEQ ID No. 1, and the nucleotide sequence of primer Rd-R is shown in SEQ ID No. 2. This application designs specific primer pairs Rd-F / Rd-R based on the ITS sequence of the root-invasive fungus isolated from the roots of *Imperata cylindrica* in Qinghai Province, and establishes a corresponding PCR detection method for specific identification. Rhypophila deceiving The results show that this method is rapid, specific, sensitive, and easy to operate. It can be used for pathogen detection, early diagnosis, and disease control of *Imperata cylindrica* root rot, providing technical support for precise monitoring and sustainable management of grassland diseases.
Owner:LANZHOU UNIV