145 results about "Azoxystrobin" patented technology

According to the nano pesticide and the preparation method and application thereof, pH response type nano particles ZIF-90 serve as a carrier, azoxystrobin is loaded to serve as a medicine, and the nano pesticide is used for preventing and treating rice sheath blight disease caused by rhizoctonia solani. When the rhizoctonia solani infects rice, oxalic acid can be generated to reduce the pH around an infected area, so that the ZIF-90 carrier skeleton and the hyaluronic acid coating are decomposed to release azoxystrobin, and the rhizoctonia solani is inhibited in a targeted manner. It is further found that ZIF-90 and azoxystrobin have synergistic bacteriostatic activity, the EC50 value of azoxystrobin to rhizoctonia solani can be greatly reduced, after ZIF-90 carrier encapsulation and hyaluronic acid end capping, the photolysis stability of azoxystrobin can be improved, the adhesive force of azoxystrobin on rice leaves can be enhanced, and the ZIF-90 and azoxystrobin compound has important application in agricultural disease control.

The invention discloses a high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation. According to the high-yield gastrodia elata planting method based on Tilia brachypoda fungus material optimization and seed ramie stress resistance activation, yield increase and quality improvement are achieved through fungus material composite strengthening treatment and a composite strain synergistic inoculation technology. The method comprises the following steps: (1) fermenting a tilia brachypoda substrate by lactobacillus plantarum and soaking in a nano-composite nutrient solution to form a high-activity bacterial material; (2) nitrogen-fixing bacteria, disease-resistant actinomycetes and armillaria mellea are mixed and inoculated according to the proportion of 3: 1: 1, nitrogen supply is improved, and the occurrence rate of black rot is reduced; 3) activating a GASA stress-resistant gene of the seed hemp through a pulsed electric field (5 kV / cm, 10 microseconds), and enabling the germination rate to reach 88.5% in combination with a calcium signal channel strengthening technology; according to the method, the yield per mu of gastrodia elata is increased by 30%-40%, the gastrodin content is increased by 30% or above, the chemical pesticide dosage is reduced by 55%, the strain passage purity is stable, the method is suitable for large-scale planting in multiple climate areas, and an innovative solution is provided for green production of traditional Chinese medicinal materials.

The present invention discloses a method for synthesizing azoxystrobin, which includes: in a water-oil system of an organic solvent and an aqueous solution of trimethylamine, the compound shown in Formula I reacts with 2-cyanophenol to obtain azoxystrobin; wherein, the molar ratio of the compound shown in Formula I to trimethylamine is 1:(0.96-2). The synthesis method of the present invention has higher reaction efficiency, does not generate CO2, avoids the risk of entrainment and overflow of materials, reduces side reactions, reduces solid waste emissions, and is green and environmentally friendly. The present invention does not need to use solid sodium carbonate or potassium carbonate, and the material reaction system is an oil-water reaction system, so the reaction efficiency is greatly improved, and the yield can also be maintained above 95%. It can take into account both efficiency (timeliness) and yield, and is easy to realize continuous reaction, providing technical support for intelligent and unmanned production; and trimethylamine can be recycled, reducing the use of one raw material, and thus does not require cumbersome treatment of mixed salts and their wastewater, and the energy consumption is significantly reduced.

The invention belongs to the field of pesticide preparation, and particularly relates to a porous chitosan-azoxystrobin nano slow-release agent as well as a preparation method and application thereof. Comprising the following steps: S1, weighing paraffin, Span-80 and Tween-80, stirring and mixing, and heating and stirring to obtain an oil phase; s2, weighing azoxystrobin raw medicine, water, 1% acetic acid and chitosan, and stirring and mixing to obtain a water phase; and S3, slowly dripping the water phase into the oil phase, and mixing and emulsifying to obtain the emulsion. And S4, slowly adding glutaraldehyde l and anhydrous acetic acid into the emulsion, and continuously heating and stirring at 60 DEG C for 1 hour to obtain the porous chitosan-azoxystrobin nano slow-release agent emulsion. And S5, centrifuging the emulsion, and alternately washing with petroleum ether, dichloromethane or trichloromethane to obtain the porous chitosan-azoxystrobin nano slow-release agent. According to the invention, porous chitosan nanoparticles with uniform morphology and particle size are prepared at room temperature by adopting an interfacial polymerization method, and when the porous chitosan nanoparticles are used in the field, the lasting period of the pesticide can be prolonged, the pesticide application frequency is reduced, and the pesticide application amount is reduced.

A shaving aid including a lubricant and a benefit agent. The benefit agent is azoxystrobin. Also provided is hair removal device with a shaving aid and the shaving aid includes azoxystrobin. The shaving aid may be on-board the hair removal device or provided separately. The shaving aid may be a solid or a liquid.

The present application relates to the field of microbiological technology, and particularly relates to a pathogenic bacterium causing root rot symptoms of red heart disease of Astragalus membranaceus and application thereof, the pathogenic bacterium is Fusarium sp. Fusarium sp. FUS-001-2025, preserved in China General Microbiological Culture Collection Center on March 16, 2026, with the preservation number of CGMCC No. 42529; it is determined to be a new recorded strain of Fusarium sp. through morphological and molecular identification, and the bactericide sensitivity determination shows that 25% silthiofam·prochloraz has the strongest inhibition effect, 25% bromothalonil, 50% azoxystrobin and 70% methylthiophanate have significant inhibition at high concentrations. The present application provides an important pathogenic bacterium resource for the pathogenic mechanism, disease resistance evaluation and development of control agents of red heart disease of Astragalus membranaceus.

The invention discloses a bactericide composition for preventing and treating earthen archaeological site mushroom diseases and application of the bactericide composition, triazole bactericides and methoxyl acrylate bactericides are used as active ingredients and compounded with a synergist, the mass ratio of triazole to azoxystrobin is 1: 1-1: 9, the content of the synergist is 0.01%-0.1% of the total mass, the content of the methoxyl acrylate bactericides is 0.01%-0.1% of the total mass, and the content of the methoxyl acrylate bactericides is 0.01%-0.1% of the total mass. The composition shows a remarkable synergistic effect, the FIC index is smaller than or equal to 0.5, and the composition has an efficient killing effect on spores, hyphae and sporocarps of common mushrooms in earthen ruins. The composition ensures safety to a ruins body material, shows a color difference value [delta] E < 3, a penetration depth greater than 5 cm, and maintains long-term stability in a humid environment for at least 12 months. The bactericide formula is systematically optimized for earthen ruins mushroom diseases for the first time, and various macro fungi including coprinus brachypoda, coprinus comatus, pleurotus ostreatus and pleurotus citrinopileatus are effectively prevented and treated. Through laboratory screening, synergistic effect testing, permeability and safety evaluation and field application verification, the composition has an excellent prevention and treatment effect.

Provided is a synthesis method for azoxystrobin, comprising: in a water-oil system of an organic solvent and an aqueous solution of trimethylamine, a compound represented by formula I reacts with 2-cyanophenol to obtain azoxystrobin, wherein the molar ratio of the compound represented by formula I to trimethylamine is 1:(0.96-2). The synthesis method has high reaction efficiency, does not produce CO2, avoids the risks of entrainment and overflow, reduces the generation of side reactions and solid waste emissions and is environmentally friendly. In addition, the method does not require the use of solid sodium carbonate or potassium carbonate, and the material reaction system is an oil-water reaction system, so that the reaction efficiency is greatly improved, and the yield can also be maintained at 95% or more, thereby taking both the efficiency (timeliness) and the yield into account and easily achieving continuous reaction, and thus providing technical support for intelligent and unmanned production. Moreover, since trimethylamine can be recycled, the use of one raw material is reduced, and further there is no need for complicated treatment for mixed salts and waste water thereof, thereby significantly reducing the energy consumption.

The application relates to the technical field of anti-transpiration agents, and discloses application of a compound in the field of plant drought resistance and a plant drought resistance preparation. At least one of the compounds of pyrisoxazole, dinotefuran, azoxystrobin and fluopicolide and the plant drought resistance preparation can significantly reduce a water transpiration rate, improve the resistance of plants to general water shortage, and reduce economic losses of plants caused by drought and water shortage.

A method for preparing a cyclodextrin-metal-organic framework (MOF) nano-sustained-release pesticide, the cyclodextrin-metal-organic framework nano-sustained-release pesticide prepared by this method, and the application of this cyclodextrin-metal-organic framework nano-sustained-release pesticide in fruit preservation. The preparation method includes 1) the preparation of γ-CD-MOF; 2) the preparation of a mixture of γ-CD and azoxystrobin; and 3) the preparation of a γ-CD-MOF / azoxystrobin nano-sustained-release pesticide. Biocompatible γ-cyclodextrin is used as the organic ligand, with K... + γ-CD-MOF materials were prepared using ion centers. The synthesis process conditions were optimized, and a universal synthesis method was established. Azoxystrobin fungicide was loaded into γ-CD-MOF materials to prepare a slow-release pesticide. The process conditions were optimized, and when the solvent ratio was 10:6, the material morphology was a uniform cubic shape with an average particle size of 1.93 μm.

The invention discloses application of carnosic acid as an agricultural bactericide and a synergist in plant diseases and a composition of the carnosic acid, and the carnosic acid has a remarkable inhibition effect on colletotrichum sorghum and is superior to a positive control drug azoxystrobin. In addition, carnosic acid is used as a synergist and is compounded with a QoI bactericide capable of inhibiting mitochondrial respiration and / or an SDHI succinate dehydrogenase inhibitor, so that the bactericidal activity can be remarkably improved. The antibacterial effect of the bactericidal composition is greatly improved by compounding the bactericidal composition with azoxystrobin, and the anti-colletotrichum sorghum result shows that when the compounding ratio of azoxystrobin to carnosic acid is 1: 4, the synergistic coefficient SR value is 1.637, and the bactericidal composition has a remarkable synergistic effect.

The invention belongs to the technical field of pesticides, and relates to 11 bactericide compositions for mainly preventing and treating crop diseases caused by pyricularia oryzae and colletotrichum gloeosporioides. The compound YJY-22 provided by the invention is novel in structure and unique in action mode, and has no cross resistance with isoprothiolane, fenoxanil, pyraclostrobin, tricyclazole, azoxystrobin, mancozeb, captan, difenoconazole, prochloraz, chlorothalonil and tebuconazole. As the action mechanism of the compound YJY-22 is different from that of the medicament, and the compound YJY-22 and the medicament have no cross resistance, the compound YJY-22 and the medicament are compounded, so that the sterilization range can be expanded, the sterilization effect can be enhanced, the drug resistance of phytopathogen can be delayed, the safety to crops is good, the prevention and control spectrum is expanded, and the requirements of pesticide reduction and synergism are met.

A fungicidal composition containing a fungicidally effective amount of the compound of Formula I, (S)-1,1-bis(4-fluorophenyl)propan-2-yl (3-acetoxy-4-methoxypicolinoyl)-Z-alaninate, and at least one fungicide selected from the group consisting of epoxiconazole, prothioconazole, azoxystrobin, pyraclostrobin, fluxapyroxad, benzovindiflupyr, and chlorothalonil, provides synergistic control of selected fungi.

The invention relates to a fungicide composition for preventing and treating peanut root rot as well as preparation and application of the fungicide composition. The invention aims to solve the technical problems of unstable field control effect, high chemical pesticide residue and high drug resistance risk of biological control in peanut root rot control. Bacillus velezensis YJ02 with good biocompatibility is compounded with azoxystrobin (a biological fungicide generates antibacterial substances and induces plant defensive enzymes, and a chemical fungicide is combined to rapidly inhibit respiration of pathogenic bacteria) to prevent and treat peanut root rot; the technical effects that the efficient synergistic control effect (for example, the control effect reaches up to 86.79%) is achieved, the application amount of chemical pesticides is reduced (for example, the dosage of azoxystrobin is only 9.82% of the conventional dosage), the drug resistance risk of pathogenic bacteria is effectively reduced, the morbidity and disease index are reduced, and environment friendliness is achieved are achieved, and high economic benefits and social benefits are achieved.

The invention belongs to the technical field of pesticide sterilization, and discloses a sterilization composition containing Cyclobutrifluram and application of the sterilization composition, the sterilization composition comprises an active component A and an active component B, the active component A is Cyclobutrifluram, and the active component B is azoxystrobin. The mass ratio of the active component A to the active component B is (1: 30)-(35: 1). The bactericidal composition disclosed by the invention has a synergistic effect on prevention and treatment of various plant pathogenic bacteria within a certain mass ratio range, can delay the generation of drug resistance of pathogenic bacteria, and has a wide application prospect in production.

The invention belongs to the technical field of crop disease and pest control, and particularly relates to application of a bactericide in controlling pepper southern blight and a method for controlling pepper southern blight. The invention provides application of a bactericide in prevention and treatment of pepper southern blight. The bactericide comprises hexaconazole and / or azoxystrobin. The varieties of the peppers comprise disease-resistant varieties and disease-susceptible varieties; the use concentration of the hexaconazole is 20 to 100 [mu] g / mL; the use concentration of the azoxystrobin is 20 to 100 [mu] g / mL. It is found that hexaconazole and azoxystrobin have a good comprehensive control effect on resistant and susceptible varieties, disease management can be conducted in a mode that the resistant varieties and the bactericide are combined when southern blight is controlled, the incidence degree of the southern blight can be effectively reduced, the usage amount of the bactericide is reduced, and stable growth of plants is maintained; the root morphology and antioxidant enzyme regulation of the disease-resistant variety are superior to those of the susceptible variety, and the protection activity control effect of the disease-resistant variety is obviously higher than that of the susceptible variety.

The invention relates to a divisional application with the application number of 202411979270.1, and discloses a synthesis method of azoxystrobin, which comprises the following steps: in a water-oil system of an organic solvent and a trimethylamine aqueous solution, reacting a compound as shown in a formula I with 2-cyanophenol to obtain azoxystrobin, wherein the molar ratio of the compound as shown in the formula I to the trimethylamine is 1: (0.96-2); a kettle type continuous reaction or a pipeline continuous reaction is adopted. The synthesis method has the advantages of higher reaction efficiency, no generation of CO2, avoidance of the risk of entrainment of overflow, reduction of side reactions, reduction of solid waste discharge, greenness and environmental protection, no need of adopting solid sodium carbonate or potassium carbonate, and great improvement of the reaction efficiency due to the fact that a material reaction system is an oil-water reaction system, and is suitable for industrial production. The yield can be maintained at 95% or above, the efficiency and the yield can be considered at the same time, continuous reaction is easy to achieve, and technical support is provided for intelligent and unmanned production; the use of one raw material is reduced, and the energy consumption is obviously reduced.

An agrochemical composition comprising component (1) and a mixture of at least two components selected from component (2), component (3) or a combination of component (2) and component (3) wherein component (1) is at least one compound of formula (I) # imgabs0 wherein R1, R2, R3, R4, R5, R6, R7 and n are defined in the specification; component (2) is at least one fungicide selected from the group consisting of: azoxystrobin, metominostrobin, picoxystrobin, trifluroxystrobin, metetropro, benzsovin diflupyr, bixafen, fluindazole, fluxafloxam, infringastatin, difenoconazol, and cyroconazol, and the component (2) is at least one fungicide selected from the group consisting of: azoxystrobin, metominostrobin, picoxystrobin, trifluroxystrobin, metetropro, benzsovin, bixafen, fluindazole, fluxafloxam, fluxafloxam, fluxafloxam, fluxafloxam, and cyroconazol And component (3) comprises at least one fungicide, different from component (1) and component (2), selected from the group consisting of (A) an inhibitor of the biosynthesis of sterols, (B) an inhibitor of the respiratory chain complex I (complex I) or complex II (complex II), (C) an inhibitor of the respiratory chain complex III (complex III), (D) a compound which may have a multi-site action, or (E) an inhibitor of histone deacetylase. The invention also relates to formulations comprising the compositions of the invention and to methods of controlling phytopathogenic fungi of plants using the agrochemical compositions of the invention.

The invention provides an epoxiconazole azoxystrobin nano suspending agent as well as a preparation method and application thereof, and belongs to the technical field of pesticides. The epoxiconazole and azoxystrobin nano suspending agent is prepared from the following components in percentage by mass: 5 to 20 percent of epoxiconazole, 10 to 30 percent of azoxystrobin, 2 to 5 percent of a wetting agent, 6 to 12 percent of a dispersing agent, 0.2 to 2 percent of a thickening agent, 1 to 5 percent of an anti-freezing agent, 0.01 to 0.5 percent of a preservative, 0.1 to 2 percent of a de-foaming agent and the balance of water. The epoxiconazole and azoxystrobin nano suspending agent is prepared by compounding epoxiconazole and azoxystrobin, has good particle size stability (the particle size D90 is less than or equal to 500nm), and can keep good particle size over-time stability under a low dilution multiple (within 100 times). The nano suspending agent can significantly enhance the prevention and treatment effect on fungal diseases, expand the prevention and treatment spectrum, delay the development of resistance to a single effective component, adapt to flight control pesticide application, and can give full play to the use advantages of the nano suspending agent.

The application discloses a propiconazole-azoxystrobin- clothianidin seed treatment suspending agent and a preparation method and application thereof, and belongs to the technical field of pesticide preparations; the agent takes propiconazole, azoxystrobin and clothianidin as active ingredients, takes beta-nicotinamide mononucleotide (NMN) as a functional antimicrobial component, and is supplemented with styrene-maleic anhydride copolymer sodium salt, fatty alcohol polyoxyethylene ether, xanthan gum, magnesium aluminum silicate, glycerol, polydimethylsiloxane defoaming agent, sodium benzoate, polyvinyl alcohol and deionized water to prepare the agent. The application is suitable for seed coating treatment for preventing and treating rice false smut, thrips and wheat sharp eyespot and aphids; NMN promotes the accumulation of phenolic compounds in the phloem of plants, and cooperates with clothianidin on the same insecticidal target, thereby significantly improving the prevention and treatment effect on aphids and thrips; the degradation product of NMN promotes the proliferation of beneficial microorganisms in the rhizosphere, improves soil enzyme activity, and realizes soil micro-ecological restoration.

This invention relates to a hybridoma cell line that secretes a monoclonal antibody against azoxystrobin and its application, belonging to the field of immunochemistry. The hybridoma cell line of this invention was deposited on November 12, 2025, at the China General Microbiological Culture Collection Center (CGMCC), located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, with accession number CGMCC No. 46742, and classified as a monoclonal cell line. The monoclonal antibody secreted by the invented hybridoma cell line exhibits good detection sensitivity (IC50) against azoxystrobin. 50 With a value of 0.049 ng / mL, it can be used for the detection of antimicrobial residues.

The invention discloses an application of fenbendazole as an agricultural fungicide in plant diseases, fenbendazole has a remarkable inhibition effect on botryosphaeria dothidea, rice sheath blight, tea tree colletotrichum and colletotrichum gloeosporioides, and is superior to positive control drugs thiabendazole and azoxystrobin. The inhibition rate of fenbendazole on the two rice sheath blight fungi is 100% under the concentration of 25 micrograms / mL. EC50 values are respectively 0.0093 [mu] g / mL and 0.0015 [mu] g / mL, and a remarkable inhibition effect is achieved. The EC50 of the compound for colletotrichum gloeosporioides and the EC50 of the compound for colletotrichum gloeosporioides are 0.085 mu g / mL and 0.18

The invention discloses an application of micafungin in preparation of a medicine for inhibiting fungi, and an application of micafungin and trifloxystrobin combined medicine in preparation of a medicine for inhibiting fungi, especially an inhibition effect on magnaporthe oryzae. Research finds that micafungin has an inhibition effect on magnaporthe oryzae. Micafungin and trifloxystrobin are combined for use and have a synergistic effect, so that the drug resistance of the trifloxystrobin drug-resistant magnaporthe oryzae can be overcome, and the antibacterial effect on the magnaporthe oryzae is improved. Research finds that micafungin has a broad-spectrum antifungal effect and also has an inhibition effect on botrytis cinerea, fusarium moniliforme, linseed fungus and other fungi besides the inhibition effect on magnaporthe oryzae.

The present invention belongs to the technical field of pesticide preparations, and specifically relates to a pesticide composition for peanut white rot and a preparation method thereof, comprising three active ingredients, Fe2 (EDTA) 3-fluoxazolidinone nanocomposite, prothioconazole and azoxystrobin, wherein the mass ratio of the Fe2 (EDTA) 3-fluoxazolidinone nanocomposite, prothioconazole and azoxystrobin is 6-8:1:1. The pesticide composition for peanut white rot in the present invention is obtained by complexing EDTANa2 with iron ions to obtain Fe2 (EDTA) 3, which can be used as a synergist of fluoxazolidinone to improve the bactericidal effect of fluoxazolidinone, and on the other hand, iron also plays an important role in the electron transfer of the mitochondrial respiratory chain, and can synergistically enhance the bactericidal effect of fluoxazolidinone, so as to achieve a better bactericidal effect.

11 fungicide compositions mainly used for controlling crop diseases caused by Pyricularia oryzae and Colletotrichum spp.. Compound YJY-22 has a novel structure and a unique mode of action, and exhibits no cross-resistance with isoprothiolane, fenoxanil, pyraclostrobin, tricyclazole, azoxystrobin, mancozeb, captan, difenoconazole, prochloraz, chlorothalonil, or tebuconazole. Because compound YJY-22 and the described agents have different mechanisms of action and have no cross-resistance, combining compound YJY-22 and the described agents not only can broaden the antifungal spectrum and enhance antifungal efficacy, but also can delay fungicide resistance of plant pathogens, ensure high crop safety, expand the control spectrum, and meet the requirement of pesticide reduction with increased efficiency.

The invention discloses an application of fluoxastrobin (Fluoxastrobin) in prevention and treatment of pathogenic fungi of crops. The fluoxatrobin has excellent bacteriostatic activity on pathogenic fungi of crops, can be used for preventing and treating diseases of important economic crops such as tea trees, vegetables, rice, fruit trees and the like caused by the pathogenic fungi, and particularly has a remarkable bacteriostatic effect on pestalotiopsis trachichioides (Pestalotiopsis trachichioides), and can be used for preventing and treating the diseases of the important economic crops such as the tea trees, the vegetables, the rice, the fruit trees and the like caused by the pathogenic fungi of the important economic crops such as the tea trees, the vegetables, the rice, the fruit trees and the like. The fluoxastrobin disclosed by the invention can delay the generation of drug resistance of germs, expand the bactericidal spectrum of pesticides and reduce the pollution of the pesticides to the environment, conforms to the policy of pesticide reduction and synergism in China, and has a wide application prospect in the field of crop disease control.

The invention provides a Tralopyril-triphenylphosphine derivative as well as a preparation method and application thereof, and belongs to the technical field of synthesis of pesticide compounds. According to the invention, Tralopyril is used as a parent structure, flexible chains with different lengths are coupled and spliced with triphenylphosphine cations to synthesize a series of new compounds, and the chemical structure is novel. Biological assay results show that in the aspect of nematode killing, toxicity of part of the compounds to caenorhabditis elegans is higher than that of a commercial medicament fosthiazate; in root-knot nematode potting activity determination, the root irrigation activity of the compound 5f is higher than that of a control agent trifluoropyridylamine. In the aspect of sterilization, the compounds have relatively high biological activity on rhizoctonia solani, sclerotinia sclerotiorum, gaeumannomyces graminis and pyricularia oryzae, and the activity of the compounds 5b, 5c, 5d and 5f on the sclerotinia sclerotiorum is higher than that of a commercial medicament azoxystrobin. The synthesized novel compound has a relatively high commercialization prospect.

The application belongs to the field of metal organic framework materials (MOFs), and particularly relates to an amino-functionalized UIO-66 (Zr) and a preparation method and application thereof. The application mainly utilizes an amino ligand containing an aminobenzoic acid group and UIO-66 (Zr) to form a complex, which has good binding capacity with azoxystrobin and other pesticide molecules and saccharide substances, and can reduce the ultraviolet band gap value, so as to improve the laser desorption / ionization efficiency of the analyte. Compared with the mass spectrum peak intensity of the organic matrix, the amino-functionalized UIO-66 (Zr) material shows lower noise and detection limit and higher signal intensity in the MALDI-TOF-MS detection, which is beneficial to realize high-sensitivity and high-throughput detection of pesticides and saccharide substances.

The invention discloses AS-PCR primers and a method for detecting drug resistance of peronophythora litchii to azoxystrobin. An AS-PCR primer capable of being used for detecting whether peronophythora litchii has drug resistance to azoxystrobin or not is obtained by comparing differential sequences of a peronophythora litchii strain capable of resisting azoxystrobin and a peronophythora litchii strain sensitive to azoxystrobin, and a method for detecting the drug resistance of peronophythora litchii to azoxystrobin is constructed based on the AS-PCR primer. The detection specificity is good, and the sensitivity is high. The AS-PCR primer and the detection method are used for amplifying a to-be-detected strain, and if a single band with the consistent size can be obtained, the detected strain is anti-azoxystrobin peronophythora litchii. Compared with a traditional detection method, the detection method disclosed by the invention is short in time consumption, simple, convenient and feasible, is beneficial to quickly monitoring early epidemic of peronophythora litchii anti-azoxystrobin groups, and provides scientific guidance for scientific prevention and control of peronophythora litchii.

The invention relates to the field of plant protection, in particular to a preparation for preventing and treating internal rot of Chinese chestnuts, a method and application. The preparation for preventing and treating internal rot of Chinese chestnuts comprises a first preparation, a second preparation and a third preparation, the first preparation contains azoxystrobin with the concentration of 2 to 4g / L; the second preparation contains pyraclostrobin with the concentration of 2 to 4 g / L; and the third preparation contains chitosan with the concentration of 1-3g / L and sodium chloride with the concentration of 1-3g / L. The preparation comprises a plurality of medicaments, different medicaments are applied in different growth periods of the Chinese chestnuts by combining the growth characteristics of the Chinese chestnuts and the morbidity characteristics of the internal rot of the Chinese chestnuts, the inhibition effect on the internal rot of the Chinese chestnuts is obvious, the intact fruit rate of chestnut kernels in the storage period of the Chinese chestnuts is increased, and the loss caused by the internal rot is reduced; a good control effect on the internal rot of the Chinese chestnuts can be achieved, especially for the internal rot of the Chinese chestnuts planted in a field.