32 results about "Tilia" patented technology

The invention belongs to the technical field of plant seedling propagation, and discloses a method for installation efficiently cutting tilia miqucliana. The method comprises the following steps of A, selecting and cultivating a female parent; B, building a cutting cultivation room; C, selecting and treating a cutting medium; D, selecting and treating cutting slips; E, cutting; F, transplanting, fertilizing and managing cutting seedlings. According to the method for installation efficiently cutting the tilia miqucliana provided by the invention, the rootage duration of the tilia miqucliana can be greatly shortened to 15d, the survival rate of cutting is 95 percent or more, and 12 times of cutting can be carried out one year, so that the problem of market resource vacancy of the tilia miqucliana is effectively solved, and the application prospect is wide.

A dry extrusion wall-breaking method for pollen comprises the following steps of: (1) soaking pollen in pure water for 30 minutes and filtering the pollen to remove dust and impurities; (2) adding the pollen obtained in step (1) into a food screw extruder to extrude the pollen at a cavity temperature of between 40 and 65 DEG C, a pressure of between 2 and 12 MPa, a rotation speed of 14 to 60 R / minpollen obtained in step (1) into a food screw extruder to extrude the pollen at a cavity temperature of between 40 and 65 DEG C, a pressure of between 2 and 12 MPa, a rotation speed of 14 to 60 R / minand a stroke of 80 to 150 centimeters, and injecting out the pollen at a high speed from a die opening with a 2 to 6 millimeter diameter for wall breaking; and (3) drying the pollen for 1 to 2 hoursunder reduced pressure and filling the pollen in bags for package to obtain the product. The pollen can be pure pollen or a mixture of any several kinds of the pollen. The pollen is selected from hawthorn pollen, rape pollen, thyme pollen, buckwheat pollen, silymarin pollen, chestnut pollen, chrysanthemum pollen, orange pollen, dandelion pollen, tilia europea, pine pollen, acacia pollen, maize polhorn pollen, rape pollen, thyme pollen, buckwheat pollen, silymarin pollen, chestnut pollen, chrysanthemum pollen, orange pollen, dandelion pollen, tilia europea, pine pollen, acacia pollen, maize pollen, sunflower pollen, astragalus sinicus pollen and the like. The pollen wall-breaking efficiency of the method is up to 99 percent. The sanitation, safety and stability of the pollen product are improved. The method is simple in process, can be operated continuously and is high in yield.

The invention discloses a simplified Tilia microphylla tissue culture method, which belongs to the technical field of plant tissue culture. The present invention comprises the following steps: 1) Collect the bud strips with axillary buds on healthy plants 20-40 days after the spring buds germinate, and after surface disinfection, intercept the stems with buds and inoculate them in induction medium for cultivation; 2) cut Remove the axillary bud bud strips in step 1), intercept the stem section with 1 bud, and inoculate it in the induction medium; 3) subculture the adventitious buds obtained in step 2); 4) grow the adventitious buds in step 3) to 2.0-3.0cm adventitious buds were transferred to the rooting medium to obtain aseptic seedlings; 5) Transfer the aseptic seedlings obtained in step 4 into the plug matrix, and cultivate the seedlings in the artificial greenhouse. The invention has strong versatility, simple operation steps, and can effectively realize the germplasm preservation and rapid propagation of different varieties of Tilia microphylla.

The invention discloses a medium for improving the induction rate of tilia callus, comprising: (1) induction medium 1: adding 2, 4-D, zeatin, KT, proline on the basis of MS medium , leucine, chitosan; and (2) induction medium 2: on the basis of MS medium supplemented with 2,4‑D, zeatin, KT, sodium thiosulfate, proline, tyrosine, Chitosan, activated carbon powder; and a culture method is disclosed. The culture medium and method of the invention can improve the speed and quality of callus formation, thereby improving the breeding efficiency.

The invention discloses a set of EST-SSR molecular markers developed based on a full-length transcriptome sequence of tilia miqueliana and relates to 27 pairs of primers in all with nucleic acid sequences shown in SEQ ID No. 1-54 of a sequence listing. The 27 pairs of EST-SSR markers disclosed by the invention are SSR amplification primers obtained through screening candidate SSR loci based on thetranscriptome sequence and carrying out designing, are verified as definite SSR markers through taking different species of this tilia as materials and carrying out amplification by selecting genomicDNA and have the advantages of rich polymorphism, stability in amplification, good repeatability, codominance, easiness in detection and the like. This group of markers fill up a blank in codominantmarkers of the tilia, belong to the fields of variety identification, genetic pedigree analysis, germ plasm resource protection, assisted breeding and the like of plants of this tilia and can be usedfor greatly promoting researches and development application of tilia resources.

A procedure and extraction of a regenerative product and a by-product for cutaneous use consisting of an infusion based on one litre of water boiled at between 95° and 100° centigrade and where, for said quantity of water, the following products must be added, quantified by weight:12 g rosemary (Rosmarinus officinalis)6 g eucalypt (Eucaliptus camaldulensis)6 g thyme (Thymus vulgaris)6 g bayleaf (Laurus nobilis)3 g linden flower (Tilia sp.)3 g fresh mint (Mentha ssp)3 g oregano (Origanum vulgare)

The invention discloses a method for improving the survival rate of wild Nanjing linden transplantation, which comprises the following steps: (1) arrangement of nursery ground; (2) transplantation of seedlings; (3) treatment of seedlings; (4) watering of rooting promoters; (5) Seedling management. The survival rate of transplanting wild Nanjing Tilia by this method is over 90.0%, which is of great significance for ex situ preservation of Nanjing Tilia germplasm gene resources, preventing the loss of excellent Nanjing Tilia genetic genes, and further research, development and utilization of wild Nanjing Tilia resources.

The invention discloses a steamed stuffed bun with sugarcane and safflower and a preparation method thereof. The steamed stuffed bun is prepared from following raw materials including, by weight, 600-700 parts of flour, 9-14 parts of a nutritional additive, 20-30 parts of carambola, 4-9 parts of fresh ginger candies, 5-10 parts of clam flesh, 10-30 parts of wax gourd, 0.6-1 part of lactic acid bacteria, 10-20 parts of grapes, 4-5 parts of salt, 2-3 parts of bagasse, 2-10 parts of white sesame seeds, 1-4 parts of jam, 0.5-0.9 parts of cordyceps sobolifera, 1-1.2 parts of safflower seeds, 0.5-1 part of eriophorum comosum, 1-2 parts of tilia omeiensis, 1-2 parts of beautiful millettia roots, 1-2 parts of monosodium glutamate, a proper amount of olive oil, a proper amount of water, and a proper amount of fresh yeast. With the added raw materials, including the carambola, the fresh ginger candies, the clam flesh and the like, the steamed stuffed bun is rich in nutrition. In addition, the steamed stuffed bun contains various Chinese herbal medicine components so that the steamed stuffed bun, when being often eaten, has effects of activating blood and moistening dryness, relieving cough and asthma, and moving qi and freeing collaterals.