43 results about "Thelephora ganbajun" patented technology

The invention relates to the technical field of thelephora ganbajun Zang polysaccharides, in particular to a thelephora ganbajun Zang exopolysaccharide. A preparation method of the thelephora ganbajun Zang exopolysaccharide includes the steps: fermenting thelephora ganbajun Zang strains TG-1; extracting the thelephora ganbajun Zang exopolysaccharide from fermentation broth; separating mixture by the aid of an anion exchange column chromatography; eluting by the aid of deionized water, 0.1 mol / L of NaCl solution and 0.3 mol / L of NaCl solution to respectively obtain thelephora ganbajun Zang exopolysaccharide components such as EPS-1, EPS-2 and EPS-3. The preservation number of the thelephora ganbajun Zang strains TG-1 is CGMCC No.12977. The thelephora ganbajun Zang EPS has good humidity preservation and antioxidant activity, antioxidant ability of the EPS-2 in the three components is highest, and the EPS is high in the humidity preservation ability. The thelephora ganbajun Zang exopolysaccharide can be widely applied to anti-aging health care products and cosmetics.

The invention relates to a PCR method identified by thelephora ganbajun Zang and a specific primer thereof. The Thelephora ganbajun Zang can be efficiently distinguished from other telephora and thelephora ganbajun Zang companion fungus with similar shape through DNA extraction, specificity PCR reaction and agarose gel electrophoresis detection, and a quick and accurate identification method is provided for the separation and purification of thelephora ganbajun. The invention has the advantages of high efficiency, speediness, strong specificity, good repeatability, and the like.

The invention discloses an industrial production method for wild thelephora ganbajun. A thelephora ganbajun strain is preserved in CGMCC, wherein the preservation number is CGMCC NO. 990(2). The production method comprises: longitudinally tearing disinfected wild thelephora ganbajun into blocks, and inoculating the wild thelephora ganbajun in a culture medium for slant culture to obtain a thelephora ganbajun strain; inoculating the thelephora ganbajun strain to a culture medium of a nutrition bag; inserting a disinfected pine root into the nutrition bag to be cultured; then dividing the second generation of thelephora ganbajun strain into number of parts and separately inoculating the pipe roots in the same culture medium to be cultured by a same method; and then culturing the whole bag in soil of the pine root, and carrying out cultivation under a certain condition till sporocarp is exposed out of the soil in 20-30 days to pick up the thelephora ganbajun. Therefore, large-scale and industrialized production of the wild thelephora ganbajun is realized. According to the thelephora ganbajun sporocarp, the original fragrance and nutrition of thelephora ganbajun are ensured. The thelephora ganbajun produced by the method disclosed by the invention is high in survival rate which can reach 99%.

The invention relates to selenium-rich chicken essence seasoning containing wild fungi and a preparation method of the selenium-rich chicken essence seasoning and belongs to the technical field of food additives. According to the selenium-rich chicken essence seasoning containing wild fungi, holy basil, tricholoma matsutake powder, russula vinosa powder, truffle powder, thelephora ganbajun zang powder and an enzymatic hydrolysate form main delicate flavor, then, roxburgh rose juice and Stanleya pinnata are used for correcting the delicate flavor, besides, disodium 5'-ribonucleotide, xylose and low-sodium salt are compounded, so that the chicken essence seasoning has unique flavor, is fragrant and delicious and contains rich nutrients; meanwhile, through compounding of the thelephora ganbajun zang powder and the Stanleya pinnata, the selenium content of the product is greatly increased, and a good health-care function is realized. Besides, the content of fat and protein in the chicken essence seasoning is greatly reduced, worry of an eater about obesity is avoided, and the selenium-rich chicken essence seasoning is easy to popularize and apply.

The invention discloses a curing method of Ganba fungus-Chinese chive flowers. The curing method comprises the following steps: curing the Chinese chive flowers, curing the Ganba fungus, curing chili peppers, making main ingredients, making ingredient liquid, curing, packaging, sterilizing and the like, wherein mass ratio of the Chinese chive flowers to the Ganba fungus is 0.5-3 to 1. The Ganba fungus and the Chinese chive flowers are innovatively matched in curing in the invention, so that the flavors and the health care effects of the two main ingredients are organically combined; the product obtained according to the invention has good taste, unique flavor and rich nutrients; special ingredient recipe and curing process are utilized, so that nitrite concentration is effectively reduced in the cured food; thus, the product is safe and healthy for eating. The Ganba fungus-Chinese chive flowers can be dipped or eaten together with rice or bread; the Ganba fungus-Chinese chive flowers can also be used as flavoring to improve taste of food; and the Ganba fungus-Chinese chive flowers has effects of eliminating fishy smell and enhancing flavor. The Ganba fungus-Chinese chive flowers is convenient for eating and has wide applied range.

The invention discloses an extraction technology of active substances in Thelephora ganbajun Zang. The extraction technology is simple, convenient and fast, is applicable to large-scale industrial production and can keep activity of chemical components in the Thelephora ganbajun Zang to the greatest extent. With the adoption of the chemical active substances extracted with the method, cancer cell growth can be inhibited remarkably, and good anti-oxidative and anti-bacterial effects are realized.

The invention discloses a lipid-lowering instant congee. The lipid-lowering instant congee is prepared from the following raw materials: rice, brown rice, quinoa, chickpea, black soya bean, balsam pear, daylily, alga, Thelephora ganbajun, pine nut, traditional Chinese medicines, salt, soybean oligosaccharide, oligomeric lactulose, Angel yeast, laminarin, laver polysaccharide and alliin. The lipid-lowering instant congee is mild in sweetness, comprehensive in nutrients, easy to absorb and capable of lowering lipid, protecting heart and cerebral vessels, promoting metabolism, eliminating toxin, maintaining beauty, promoting intestinal functions, resisting oxidation and enhancing immunity; coarse grain powder and fruit and vegetable pulp are subjected to fermentation so as to obtain ferment, so the lipid-lowering instant congee is rich in fermentation fragrance and can promotion by the body; through freeze drying of the conge, ferment activity is maintained, fat absorption is reduced, and the content of blood fat is lowered; a plurality of traditional Chinese medicine extract and a nutritional agent are added and are cooperated with the ferment to exert effect on prevention and treatment of cardio-cerebrovascular diseases; and since the congee is in the form of dried powder, the congee is convenient to carry and eat and rapid in rehydration, which accords with a fast-pace living mode.

An artificial cultivation method of Thelephora ganbajun is provided, solving the problem that the previous bacterial liquid infection has low efficiency. A culture medium simulating the natural environment is used to culture hypha, so that breeding promotion efficiency is improved for Thelephora ganbajun and a better survival environment is provided for Thelephora ganbajun without affecting the growth of pine trees; therefore, Thelephora ganbajun can grow to a bigger colony, breeding is scientifically promoted, pinewood is scientifically managed, and the natural environment is better protected.

The invention discloses a selenium-enriching type solid compound condiment and a preparation method thereof. The selenium-enriching type solid compound condiment is prepared from the following components in parts by mass: 18-29 parts of a selenium-enriching component, 6-10 parts of Chinese prickly ash, 4-7 parts of star aniseed powder, 3-8 parts of ground pepper, 3-7 parts of liquorice root powder, 2-5 parts of powdered cinnamon, 3-6 parts of funnel powder, 2-5 parts of clove powder, 2-5 parts of dried hawthorn fruit powder, 6-11 parts of shrimp meal, 7-12 parts of beef powder, 8-14 parts of chicken powder, 6-10 parts of Thelephora ganbajun Zang powder, 7-12 parts of collybia albuminosa powder, 5-8 parts of ginger powder, 6-10 parts of yeast extract, 76-84 parts of sodium chloride, 32-41 parts of potassium chloride, 12-18 parts of refined chicken fat, 3-7 parts of disodium 5'-ribonucleotide, 7-11 parts of astaxanthin and 4-8 parts of silkworm chrysalis meal. The preparation method comprises the following steps: mixing the sodium chloride, the potassium chloride and the yeast extract in a stirring kettle; adding water and dissolving the materials, and heating to 80 DEG C; stirring and dissolving; heating and melting the refined chicken fat and adding the melted refined chicken fat and the residual components into the stirring kettle; stirring for 30 minutes; and spraying and drying by adopting a centrifugal spraying drying tower to obtain granular products. The condiment disclosed by the invention has abundant tastes, contains low sodium and high potassium and contains rich organic-state selenium.

The invention discloses a functional agent containing a thelephora Ganbajun extract and a cigarette filter tip prepared by utilizing the functional agent, belonging to the technical field of production of cigarettes. By the process for generating a thelephora Ganbajun characteristic potential fragrance substance from a relevant precursor substance through a biotechnology, the precursor substance generates enzymatic conversion reaction and is purified to obtain a thelephora Ganbajun natural potential fragrance substance; and the thelephora Ganbajun potential fragrance substance is prepared into the functional agent according to a certain proportion. The functional agent prepared by the extract containing thelephora Ganbajun is added to a chitosan fiber for preparing a cigarette filter tip core line. The cigarette filter tip prepared by the scheme can generate special fragrance during smoking, enable smokers to feel strong fragrance and improve taste of cigarettes; meanwhile, the chitosan fiber can adsorb and remove heavy metals in smoke.

The invention relates to the technical field of thelephora ganbajun polysaccharide, and particularly relates to a thelephora ganbajun mycelium polysaccharide. The preservation number is CGMCC No.12977; a thelephora ganbajun strain TG-1 is fermented in a liquid culture medium; and polysaccharide is extracted from mycelium and is eluted by using deionized water and 0.05, 0.1 or 0.3mol / L of NaCl solution after chromatographic separation to obtain MPS-1, MPS-2, MPS-3 and MPS-4 separately. The mycelium grows vigorously when the TG-1 is fermented and cultivated in liquid, and the mycelium is high in polysaccharide yield, and is of very great significance in development and utilization of thelephora ganbajun and massive production of the thelephora ganbajun mycelium polysaccharide. The antioxidant ability of the MPS-2 is the highest, the antioxidant ability of the MPS-3, the MPS-4 and the MPS-1 are secondary, and a theoretical basis is laid for activity analysis of the polysaccharide and development of polysaccharide foods with an anti-aging function; and the thelephora ganbajun mycelium polysaccharide also has excellent moisturizing ability.

The invention provides a method for improving the yield of thelephora ganbajun mycelia and polysaccharides and application of the thelephora ganbajun polysaccharides. Thelephora ganbajun strains withthe number of BNCC 230987 are subjected to fermented culture in a liquid culture medium with zinc salt, after culture and separation, the thelephora ganbajun mycelia are obtained, endopolysaccharidesof the mycelia are extracted in the thelephora ganbajun mycelia, and exopolysaccharides are extracted in a fermentation liquid obtained after separation of the mycelia. A yield improvement way is provided for producing the thelephora ganbajun mycelia and the polysaccharides by using a liquid fermentation method, when the zinc concentration is 400 mg / L, the yield of the thelephora ganbajun mycelia,the yield of the endopolysaccharides of the mycelia and the yield of the exopolysaccharides are increased by 49.85%, 119.21% and 361.11% respectively, and other thelephora ganbajun strains cannot reach the yield. The thelephora ganbajun polysaccharides prepared by using the method has an anti-inflammatory effect and is free of side and toxic effect, and the thelephora ganbajun polysaccharides canbe developed into health care foods or cosmetics or drugs with anti-inflammatory and anti-oxidative functions.

The invention discloses a preparation process and device of a sol capable of releasing the dormancy of a dormant thelephora ganbajun fruiting body to enter into a growing period. The preparation process comprises the following steps: leaching and extracting rosin after microorganisms are killed in anhydrous ethanol in advance to obtain a brownish yellow anhydrous ethanol rosin solution, mixing theobtained anhydrous ethanol rosin solution with sterile water, preparing a sol by adopting a centrifugal spraying method, and adding calcium nitrate and xylose into the prepared sol to prepare a sol capable of releasing dormant thelephora ganbajun; the preparation device comprises a liquid storage tank, an anhydrous ethanol storage tank and a Nobes steam generator; a fractional sterilization method is adopted, therefore, the air tightness is good; the sol can promote the releasing of dormant thelephora ganbajun, and provide a favorable foundation for the research and promotion technology of thelephora ganbajun, and as a result, the propagules of thelephora ganbajun are further expanded, the forest biome is improved and the restoration of destroyed forest lands is promoted.

The present invention discloses dietary therapeutic powder having main effects of preventing cancers and a preparation method thereof. The dietary therapeutic powder comprises raw materials of corn kernels, chickpea kernels, purple sweet potato fleshes, carrot fleshy roots, sweet potatoes, broccoli, blueberry fruits, black cerasus humilis fruits, rubus parviflolius fruits, semen armeniacae amarae, hazelnut kernels, halophilic algae, saarcodon imbricatum, thelephora ganbajun, agaricus blazei, flammulina velutipes, shii-take, Curasao aloe vera leaves at more than 3 years and Milpa Alta edible cactus fleshy stems. The various non-polluting natural edible ingredients are finely selected and subjected to a scientific compatibility. The simple physical processing method is used to maximally retain active ingredients in the raw materials. The dietary therapeutic powder is good in palatability and free of any side effects. The dietary therapeutic powder has good effects in preventing the cancers and at the same time has good performances in various aspects of adjuvantly treating gastrointestinal diseases, diabetes, dermatosis, heart diseases and eye diseases, lowering high blood lipids, high blood pressure and high blood sugar, preventing allergies, preventing radiation, preventing bacteria, preventing aging, etc.

A making method of pickled leek flower with Thelephora ganbajun includes: weighing, by weight, 6-8 parts of fresh Thelephora ganbajun, 18-22 parts of fresh leek flower, 22-28 parts of fresh chili pepper, 6-8 parts of fresh ginger, 8-12 parts of brown sugar, 7-9 parts of salt, 2-5 parts of honey, and 2-4 parts of Thelephora ganbajun wine; washing the fresh Thelephora ganbajun, airing, adding the honey, stirring until Thelephora ganbajun shines, mixing with the fresh leek flower, the fresh chili pepper, the fresh ginger, the brown sugar and the salt, adding the Thelephora ganbajun wine, mixing well, loading in a jar, and sealing and pickling for 30 days and longer. The making method has the advantages that the amount of added Thelephora ganbajun can be greatly increased, fungal aroma is strong, Thelephora ganbajun never softens, the fragrant and crispy taste of Thelephora ganbajun is maintained constantly, and the pickled leek flower with Thelephora ganbajun having good fungal aroma andtaste is made by pickling.

The invention belongs to the chemical field and the medical field, and relates to application of thelephora ganbajun in preparation of antitumor drugs. Tumor cells comprise hepatocellular carcinoma cells, lung adenocarcinma cells and pancreatic cancer cells. The thelephora ganbajun belongs to a natural product, is small in toxic and side effects, high in bioavailability and stable in performance,and has clinical service values. Small molecular compounds are developed as novel antitumor drugs or auxiliary elements of the novel antitumor drugs, are obvious in antitumor effect and are environment-friendly. The application of thelephora ganbajun in preparation of antitumor drugs provides a novel way and a novel means for treating and curing tumor.

The invention relates to healthcare noodles which are prepared from the following raw materials in parts by mass: 15-20 parts of cyclocarya paliurus leaf powder, 15-20 parts of kelp powder, 1-10 parts of yam powder, 1-5 parts of radix polygonati officinalis powder, 2-4 parts of konjac flour, 1-3 parts of desert cistanche powder, 1-3 parts of thelephora ganbajun powder, 70-80 parts of strong flour, 1-3 parts of table salt and the balance of water. A preparation method comprises the steps of solution preparation, efficacy mixing, mixing and proportioning, machine noodle making, light baking and setting, etc. Without accessories such as spice, sweetening agent and preservative, the blood sugar reducing healthcare noodles provided by the invention have high safety and can realize obvious functions of reducing blood sugar concentration, preventing arteriosclerosis, enhancing immunity, treating constipation, frequent micturition and urgent micturition and the like after being eaten for a long time; moreover, the requirements on equipment are not high, the cost is relatively low, and the operability is high; and therefore, the healthcare noodles are suitable for large-scale production of noodle factories.

The invention provides wild mushroom powder and a preparation method thereof. The wild mushroom powder comprises raw materials in parts by weight as follows: 250-300 parts of bolete, 120-140 parts of Russula virescens, 40-60 parts of Thelephora ganbajun, 8-12 parts of a yeast extract, 80-95 parts of table salt, 80-90 parts of sodium glutamate, 5-8 parts of hydrolyzed vegetable protein, 2 parts of vitamin E, 1 part of d-sodium erythorbate, 0.2-0.4 parts of papain, 0.1-0.3 parts of a flavor enzyme, 0.3-0.5 parts of cellulase and 0.3-0.5 parts of hemicellulose. According to the wild mushroom powder and the preparation method thereof, through comprehensive utilization of the cellulase, the hemicellulose and the flavor enzyme, cell walls of wild mushrooms are broken, rich nutritional substances in the wild mushrooms are extracted, the edible value of the wild mushrooms is increased, and effects that the wild mushrooms are stored for a long time and are convenient to eat are realized.

The invention discloses brain-strengthening children porridge which is prepared from the following raw materials: rice, millet, red rice, soybeans, black beans, needle mushroom, day lily, athyrium multidentatum, thelephora ganbajun, walnut kernels, traditional Chinese medicines, table salt, mannitol, fructo-oligosaccharide, angel yeast and vitamin B. The brain-strengthening children porridge disclosed by the invention has the advantages of abundant raw materials, moderate sweetness, comprehensive nutrition and easiness in absorption and has the functions of strengthening the brain and improving intelligence, enhancing memory ability, promoting metabolism, detoxifying for beauty, promoting intestinal functions, resisting oxidation and improving immunity; coarse food grain powder and fruit / vegetable pulp are fermented to obtain an enzyme which has rich fermentation aroma and promotes body absorption; through freeze drying, the enzyme activity is maintained, and the acid-base balance of the body can be kept to keep a healthy state; multiple traditional Chinese medicine extracts are added to coordinate with the enzyme so as to promote the functions of the immune system and resist bacteria, virus and cancer; and in a form of dry powder, the porridge is convenient to carry and eat, can be quickly re-watered and conforms to the fast-pace lifestyle.

The invention relates to a method for forming mycorrhizae after two types of ectotrophic mycorrhiza fungi, namely sparassis crispa and tricholoma matsutake infect seedlings of several kinds of pinus plants and belongs to the fields of artificial ectotrophic mycorrhiza fungus infection seedling-growing application technology and agricultural cultivation technology. The method comprises the steps of 1, acquisition and cultivation of sterile seeds of the pinus plants; 2, selection and treatment of a culture substrate; 3, selection and treatment of seedling-growing hole trays; 4, preparation before infection treatment; 5, infection treatment of the sparassis crispa and the tricholoma matsutake; 6, seedling stage management after infection treatment. The seedlings with the mycorrhizae successfully formed through infection grow stronger than the seedlings not subjected to infection, the leaf color is thick green, euphylla grows out in advance, disease resistance is enhanced, the symbiotic relationship between the sparassis crispa, the tricholoma matsutake and the several kinds of pinus plants under controllable conditions is creatively and successfully created, a study case can be provided for the symbiotic relationship between plants and fungi in the study of the ectotrophic mycorrhiza fungi, and a solid scientific foundation is laid for realizing artificial cultivation of the ectotrophic mycorrhiza fungi.

The invention discloses deep-fried bolete canned foods. The deep-fried bolete canned foods are made through the following steps of deep-frying fresh bolete at low temperature for a short time, then adding seasoning prepared by fine grinding to obtain fine powder, adding fresh thelephora ganbajun zang, after blending, performing canning, adding hot oil of 90-100 DEG C, and after performing sealing,performing steam heating, so that on one hand, sterilizing and disinfection can be performed, the deep-fried bolete is safe to eat, and the quality guaranteeing time is long. The deep-fried and the thelephora ganbajun zang are reprocessed at 125-130 DEG C, so that unique and rich fragrance of the bolete and the thelephora ganbajun zang can be stimulated, and the deep-fried bolete canned foods arefresh and delicious in flavor, rich in nutrients and long in quality guaranteeing time.

The invention provides tea wine and a preparing method thereof. The tea wine is mainly prepared from, by weight, 50-100 parts of bagasse pith, 1-2 parts of starch, 10-20 parts of tea leaves, 5-8 partsof thelephora ganbajun and 3-5 parts of rhizoma polygonati. The preparing method of the tea wine mainly includes the following steps of mixing and wetting the impurity-removed bagasse pith with water, adding the starch to the bagasse pith for constant-temperature fermentation, smashing dry tea leaves to obtain tea powder, weighing the tea powder, thelephora ganbajun, rhizoma polygonati, bagasse pith and starch fermentation objects, adding base wine for soaking for 20-30 days to obtain soaked wine, and conducting filtering and saccharifying, low-temperature constant-pressure filling and sterilizing on the soaked wine to obtain the tea wine. The tea wine is mellow and sweet in taste and fragrant in smell, is free of side effects after being drunk and has the functions of improving the immunity, reducing blood lipid, promoting digestion, tonifying the stomach and reducing the morbidity of cardia-cerebrovascular diseases and heart diseases after being drunk properly.

The invention discloses a method for determining the content of Thelephora ganbajun mycelia in soil. The method comprises the following steps: in a Thelephora ganbajun growing field, taking seven soilsamples at different depths from soil in a circular area where sporocarp is taken as a center, a radius is 5 cm and a depth is 14 cm through a five-point method, wherein a depth of 2 cm is taken as one soil depth, putting the seven soil samples into prepared sterile sampling bags, numbering the sterile sampling bags, and storing the soil samples at a temperature of -80 DEG C; carrying out DNA extraction on the soil sample to obtain a liquid which is a mixed liquid of DNAs of soil fungi, and naturally cooling the mixed liquid of the DNAs of the soil fungi at -20 DEG C for later use; and carrying out a qPCR test by adopting a primer Tg, and substituting a detected Ct value into a standard curve equation to calculate the mycelium content of the soil. The method can achieve the purpose of rapidly detecting the content of Thelephora ganbajun mycelia in soil, and is simple and efficient.

The invention provides thelephora ganbajun Zang distilled liquor and a making process thereof, and belongs to the technical field of wine brewing.The making process of the thelephora ganbajun Zang distilled liquor comprises the following steps that cleaned thelephora ganbajun Zang is soaked in base liquor for 15-365 days, and soaking liquid containing thelephora ganbajun Zang is formed; and distilling the soak solution, controlling the temperature of the obtained wine to be less than or equal to 25 DEG C during distillation, and keeping the distilled wine with the alcoholic strength of 30-65% vol. According to the preparation process, the thelephora ganbajun Zang is distilled after being soaked, so that the taste of the wine can be improved, most effective components in the thelephora ganbajun Zang are reserved, and the nutrition is rich.

The invention discloses a method for artificially forming a thelephora ganbajun fungal colony (shiro). The method includes the following steps: selecting an appropriate forest land and young pines, artificially excavating a fungal colony (shiro), adding highly-dispersing resin particles and amines and amides extracted from rotten thelephora ganbajun into the soil of the fungal colony (shiro), artificially monitoring and maintaining, promoting adaptability of thelephora ganbajun propagules to the soil, rapidly promoting the growth of a symbiosis system for fungi and plants, and forming the fungal colony (shiro) that enables thelephora ganbajun trophozoites to grow normally and hyphae to be active. The method has the advantages of simple operation, high survival probability of inoculation, rapid symbiosis system formation, and short period of forming the fungal colony (shiro). Resin sol is utilized to act with soil and form an area distributed with organic matter and minerals and inorganic salt; information transferring rate between pines and thelephora ganbajun is improved; the adsorption of the soil expedites the generation of microorganism under trees; the water-retaining capability of the soil is improved; circulating speed of substances in forest land and energy are actually quickened.

The invention discloses a wild thelephora ganbajun zang compound condiment which is formed by the following components in parts by weight: 12-15 parts of wild thelephora ganbajun zang, 10-20 parts of beef powder, 5-10 parts of shrimp powder, 12-25 parts of sucrose, 3-5 parts of pepper, 3-5 parts of paprika powder, 3-4 parts of chili powder, 3-5 parts of ginger powder, 1-4 parts of garlic powder, 3-5 parts of salt, 2-3 parts of sucrose, 5-8 parts of rosemary, 10-15 parts of plant oil, 12-18 parts of papain, 6-9 parts of flavorzyme, 3-5 parts of medlar powder and 2-3 parts of red date powder. A preparation method of the wild thelephora ganbajun zang compound condiment comprises the following steps of: 1) adding the papain and the flavorzyme into the wild thelephora ganbajun zang to carry out enzymolysis and further treating to obtain wild thelephora ganbajun zang powder; and 2) heating the plant oil to be at 200-220 DEG C and sequentially adding all the raw materials to be uniformly mixed to obtain the wild thelephora ganbajun zang compound condiment. The condiment disclosed by the invention has special aroma, good flavor, and delicious mouth feeling and taste, and contains substances including a plurality of delicious amino acids, low-molecular-weight peptides and the like, so that the human immunity can be enhanced.

The invention discloses a Sagittaria trifolia slice with a Thelephora ganbajun Zang taste and a preparation method thereof and belongs to the technical field of food processing. The Sagittaria trifolia slice solves the problem that the existing Sagittaria trifolia slice is prepared by frying and is bad for human health after long-term use. The Sagittaria trifolia slice is prepared from 80-90 parts by weight of Sagittaria trifolia starch, 20-30 parts by weight of Thelephora ganbajun Zang, 20-25 parts by weight of egg, 4-6 parts by weight of peanut oil, 2-3 parts by weight of table salt and 0.3-0.6 parts by weight of sodium bicarbonate. The preparation method comprises raw material selection, gelatinized starch preparation, baking raw material preparation, microwave baking and packaging. The Sagittaria trifolia slice is a non-fried food and is conducive to human health. The Sagittaria trifolia slice has a cool and refreshing taste of Sagittaria trifolia and a thick Thelephora ganbajun Zang fragrance. The Sagittaria trifolia and Thelephora ganbajun Zang produce synergism and have antioxidation effects. The Sagittaria trifolia slice is a good medicinal food.

The invention provides a specific DNA fragment for identification of thelephora ganbajun, an amplification primer, a preparation method and application thereof, and belongs to the technical field of biotechnology and enzyme engineering. A nucleotide sequence of the specific DNA fragment for the identification of the thelephora ganbajun is shown in an SEQ ID No.1 sequence table. The primer for amplifying the specific DNA fragment includes a 158F primer and a 437R primer. The specific DNA fragment for the identification of the thelephora ganbajun, the amplification primer or the application of the thelephora ganbajun specific DNA fragment obtained by the preparation method in the identification of the thelephora ganbajun can quickly and accurately complete the identification of the thelephora ganbajun.