43 results about "Thelephora ganbajun" patented technology

The invention relates to the technical field of thelephora ganbajun Zang polysaccharides, in particular to a thelephora ganbajun Zang exopolysaccharide. A preparation method of the thelephora ganbajun Zang exopolysaccharide includes the steps: fermenting thelephora ganbajun Zang strains TG-1; extracting the thelephora ganbajun Zang exopolysaccharide from fermentation broth; separating mixture by the aid of an anion exchange column chromatography; eluting by the aid of deionized water, 0.1 mol/L of NaCl solution and 0.3 mol/L of NaCl solution to respectively obtain thelephora ganbajun Zang exopolysaccharide components such as EPS-1, EPS-2 and EPS-3. The preservation number of the thelephora ganbajun Zang strains TG-1 is CGMCC No.12977. The thelephora ganbajun Zang EPS has good humidity preservation and antioxidant activity, antioxidant ability of the EPS-2 in the three components is highest, and the EPS is high in the humidity preservation ability. The thelephora ganbajun Zang exopolysaccharide can be widely applied to anti-aging health care products and cosmetics.

The invention relates to a PCR method identified by thelephora ganbajun Zang and a specific primer thereof. The Thelephora ganbajun Zang can be efficiently distinguished from other telephora and thelephora ganbajun Zang companion fungus with similar shape through DNA extraction, specificity PCR reaction and agarose gel electrophoresis detection, and a quick and accurate identification method is provided for the separation and purification of thelephora ganbajun. The invention has the advantages of high efficiency, speediness, strong specificity, good repeatability, and the like.

The invention discloses an extraction technology of active substances in Thelephora ganbajun Zang. The extraction technology is simple, convenient and fast, is applicable to large-scale industrial production and can keep activity of chemical components in the Thelephora ganbajun Zang to the greatest extent. With the adoption of the chemical active substances extracted with the method, cancer cell growth can be inhibited remarkably, and good anti-oxidative and anti-bacterial effects are realized.

The invention discloses a selenium-enriching type solid compound condiment and a preparation method thereof. The selenium-enriching type solid compound condiment is prepared from the following components in parts by mass: 18-29 parts of a selenium-enriching component, 6-10 parts of Chinese prickly ash, 4-7 parts of star aniseed powder, 3-8 parts of ground pepper, 3-7 parts of liquorice root powder, 2-5 parts of powdered cinnamon, 3-6 parts of funnel powder, 2-5 parts of clove powder, 2-5 parts of dried hawthorn fruit powder, 6-11 parts of shrimp meal, 7-12 parts of beef powder, 8-14 parts of chicken powder, 6-10 parts of Thelephora ganbajun Zang powder, 7-12 parts of collybia albuminosa powder, 5-8 parts of ginger powder, 6-10 parts of yeast extract, 76-84 parts of sodium chloride, 32-41 parts of potassium chloride, 12-18 parts of refined chicken fat, 3-7 parts of disodium 5'-ribonucleotide, 7-11 parts of astaxanthin and 4-8 parts of silkworm chrysalis meal. The preparation method comprises the following steps: mixing the sodium chloride, the potassium chloride and the yeast extract in a stirring kettle; adding water and dissolving the materials, and heating to 80 DEG C; stirring and dissolving; heating and melting the refined chicken fat and adding the melted refined chicken fat and the residual components into the stirring kettle; stirring for 30 minutes; and spraying and drying by adopting a centrifugal spraying drying tower to obtain granular products. The condiment disclosed by the invention has abundant tastes, contains low sodium and high potassium and contains rich organic-state selenium.

The invention discloses a functional agent containing a thelephora Ganbajun extract and a cigarette filter tip prepared by utilizing the functional agent, belonging to the technical field of production of cigarettes. By the process for generating a thelephora Ganbajun characteristic potential fragrance substance from a relevant precursor substance through a biotechnology, the precursor substance generates enzymatic conversion reaction and is purified to obtain a thelephora Ganbajun natural potential fragrance substance; and the thelephora Ganbajun potential fragrance substance is prepared into the functional agent according to a certain proportion. The functional agent prepared by the extract containing thelephora Ganbajun is added to a chitosan fiber for preparing a cigarette filter tip core line. The cigarette filter tip prepared by the scheme can generate special fragrance during smoking, enable smokers to feel strong fragrance and improve taste of cigarettes; meanwhile, the chitosan fiber can adsorb and remove heavy metals in smoke.

The invention relates to the technical field of thelephora ganbajun polysaccharide, and particularly relates to a thelephora ganbajun mycelium polysaccharide. The preservation number is CGMCC No.12977; a thelephora ganbajun strain TG-1 is fermented in a liquid culture medium; and polysaccharide is extracted from mycelium and is eluted by using deionized water and 0.05, 0.1 or 0.3mol/L of NaCl solution after chromatographic separation to obtain MPS-1, MPS-2, MPS-3 and MPS-4 separately. The mycelium grows vigorously when the TG-1 is fermented and cultivated in liquid, and the mycelium is high in polysaccharide yield, and is of very great significance in development and utilization of thelephora ganbajun and massive production of the thelephora ganbajun mycelium polysaccharide. The antioxidant ability of the MPS-2 is the highest, the antioxidant ability of the MPS-3, the MPS-4 and the MPS-1 are secondary, and a theoretical basis is laid for activity analysis of the polysaccharide and development of polysaccharide foods with an anti-aging function; and the thelephora ganbajun mycelium polysaccharide also has excellent moisturizing ability.

The invention provides a method for improving the yield of thelephora ganbajun mycelia and polysaccharides and application of the thelephora ganbajun polysaccharides. Thelephora ganbajun strains withthe number of BNCC 230987 are subjected to fermented culture in a liquid culture medium with zinc salt, after culture and separation, the thelephora ganbajun mycelia are obtained, endopolysaccharidesof the mycelia are extracted in the thelephora ganbajun mycelia, and exopolysaccharides are extracted in a fermentation liquid obtained after separation of the mycelia. A yield improvement way is provided for producing the thelephora ganbajun mycelia and the polysaccharides by using a liquid fermentation method, when the zinc concentration is 400 mg/L, the yield of the thelephora ganbajun mycelia,the yield of the endopolysaccharides of the mycelia and the yield of the exopolysaccharides are increased by 49.85%, 119.21% and 361.11% respectively, and other thelephora ganbajun strains cannot reach the yield. The thelephora ganbajun polysaccharides prepared by using the method has an anti-inflammatory effect and is free of side and toxic effect, and the thelephora ganbajun polysaccharides canbe developed into health care foods or cosmetics or drugs with anti-inflammatory and anti-oxidative functions.

The invention discloses a preparation process and device of a sol capable of releasing the dormancy of a dormant thelephora ganbajun fruiting body to enter into a growing period. The preparation process comprises the following steps: leaching and extracting rosin after microorganisms are killed in anhydrous ethanol in advance to obtain a brownish yellow anhydrous ethanol rosin solution, mixing theobtained anhydrous ethanol rosin solution with sterile water, preparing a sol by adopting a centrifugal spraying method, and adding calcium nitrate and xylose into the prepared sol to prepare a sol capable of releasing dormant thelephora ganbajun; the preparation device comprises a liquid storage tank, an anhydrous ethanol storage tank and a Nobes steam generator; a fractional sterilization method is adopted, therefore, the air tightness is good; the sol can promote the releasing of dormant thelephora ganbajun, and provide a favorable foundation for the research and promotion technology of thelephora ganbajun, and as a result, the propagules of thelephora ganbajun are further expanded, the forest biome is improved and the restoration of destroyed forest lands is promoted.

The invention belongs to the chemical field and the medical field, and relates to application of thelephora ganbajun in preparation of antitumor drugs. Tumor cells comprise hepatocellular carcinoma cells, lung adenocarcinma cells and pancreatic cancer cells. The thelephora ganbajun belongs to a natural product, is small in toxic and side effects, high in bioavailability and stable in performance,and has clinical service values. Small molecular compounds are developed as novel antitumor drugs or auxiliary elements of the novel antitumor drugs, are obvious in antitumor effect and are environment-friendly. The application of thelephora ganbajun in preparation of antitumor drugs provides a novel way and a novel means for treating and curing tumor.

The invention relates to healthcare noodles which are prepared from the following raw materials in parts by mass: 15-20 parts of cyclocarya paliurus leaf powder, 15-20 parts of kelp powder, 1-10 parts of yam powder, 1-5 parts of radix polygonati officinalis powder, 2-4 parts of konjac flour, 1-3 parts of desert cistanche powder, 1-3 parts of thelephora ganbajun powder, 70-80 parts of strong flour, 1-3 parts of table salt and the balance of water. A preparation method comprises the steps of solution preparation, efficacy mixing, mixing and proportioning, machine noodle making, light baking and setting, etc. Without accessories such as spice, sweetening agent and preservative, the blood sugar reducing healthcare noodles provided by the invention have high safety and can realize obvious functions of reducing blood sugar concentration, preventing arteriosclerosis, enhancing immunity, treating constipation, frequent micturition and urgent micturition and the like after being eaten for a long time; moreover, the requirements on equipment are not high, the cost is relatively low, and the operability is high; and therefore, the healthcare noodles are suitable for large-scale production of noodle factories.

The invention provides wild mushroom powder and a preparation method thereof. The wild mushroom powder comprises raw materials in parts by weight as follows: 250-300 parts of bolete, 120-140 parts of Russula virescens, 40-60 parts of Thelephora ganbajun, 8-12 parts of a yeast extract, 80-95 parts of table salt, 80-90 parts of sodium glutamate, 5-8 parts of hydrolyzed vegetable protein, 2 parts of vitamin E, 1 part of d-sodium erythorbate, 0.2-0.4 parts of papain, 0.1-0.3 parts of a flavor enzyme, 0.3-0.5 parts of cellulase and 0.3-0.5 parts of hemicellulose. According to the wild mushroom powder and the preparation method thereof, through comprehensive utilization of the cellulase, the hemicellulose and the flavor enzyme, cell walls of wild mushrooms are broken, rich nutritional substances in the wild mushrooms are extracted, the edible value of the wild mushrooms is increased, and effects that the wild mushrooms are stored for a long time and are convenient to eat are realized.

The invention discloses brain-strengthening children porridge which is prepared from the following raw materials: rice, millet, red rice, soybeans, black beans, needle mushroom, day lily, athyrium multidentatum, thelephora ganbajun, walnut kernels, traditional Chinese medicines, table salt, mannitol, fructo-oligosaccharide, angel yeast and vitamin B. The brain-strengthening children porridge disclosed by the invention has the advantages of abundant raw materials, moderate sweetness, comprehensive nutrition and easiness in absorption and has the functions of strengthening the brain and improving intelligence, enhancing memory ability, promoting metabolism, detoxifying for beauty, promoting intestinal functions, resisting oxidation and improving immunity; coarse food grain powder and fruit/vegetable pulp are fermented to obtain an enzyme which has rich fermentation aroma and promotes body absorption; through freeze drying, the enzyme activity is maintained, and the acid-base balance of the body can be kept to keep a healthy state; multiple traditional Chinese medicine extracts are added to coordinate with the enzyme so as to promote the functions of the immune system and resist bacteria, virus and cancer; and in a form of dry powder, the porridge is convenient to carry and eat, can be quickly re-watered and conforms to the fast-pace lifestyle.

The invention discloses deep-fried bolete canned foods. The deep-fried bolete canned foods are made through the following steps of deep-frying fresh bolete at low temperature for a short time, then adding seasoning prepared by fine grinding to obtain fine powder, adding fresh thelephora ganbajun zang, after blending, performing canning, adding hot oil of 90-100 DEG C, and after performing sealing,performing steam heating, so that on one hand, sterilizing and disinfection can be performed, the deep-fried bolete is safe to eat, and the quality guaranteeing time is long. The deep-fried and the thelephora ganbajun zang are reprocessed at 125-130 DEG C, so that unique and rich fragrance of the bolete and the thelephora ganbajun zang can be stimulated, and the deep-fried bolete canned foods arefresh and delicious in flavor, rich in nutrients and long in quality guaranteeing time.

The invention provides tea wine and a preparing method thereof. The tea wine is mainly prepared from, by weight, 50-100 parts of bagasse pith, 1-2 parts of starch, 10-20 parts of tea leaves, 5-8 partsof thelephora ganbajun and 3-5 parts of rhizoma polygonati. The preparing method of the tea wine mainly includes the following steps of mixing and wetting the impurity-removed bagasse pith with water, adding the starch to the bagasse pith for constant-temperature fermentation, smashing dry tea leaves to obtain tea powder, weighing the tea powder, thelephora ganbajun, rhizoma polygonati, bagasse pith and starch fermentation objects, adding base wine for soaking for 20-30 days to obtain soaked wine, and conducting filtering and saccharifying, low-temperature constant-pressure filling and sterilizing on the soaked wine to obtain the tea wine. The tea wine is mellow and sweet in taste and fragrant in smell, is free of side effects after being drunk and has the functions of improving the immunity, reducing blood lipid, promoting digestion, tonifying the stomach and reducing the morbidity of cardia-cerebrovascular diseases and heart diseases after being drunk properly.

The invention discloses a method for determining the content of Thelephora ganbajun mycelia in soil. The method comprises the following steps: in a Thelephora ganbajun growing field, taking seven soilsamples at different depths from soil in a circular area where sporocarp is taken as a center, a radius is 5 cm and a depth is 14 cm through a five-point method, wherein a depth of 2 cm is taken as one soil depth, putting the seven soil samples into prepared sterile sampling bags, numbering the sterile sampling bags, and storing the soil samples at a temperature of -80 DEG C; carrying out DNA extraction on the soil sample to obtain a liquid which is a mixed liquid of DNAs of soil fungi, and naturally cooling the mixed liquid of the DNAs of the soil fungi at -20 DEG C for later use; and carrying out a qPCR test by adopting a primer Tg, and substituting a detected Ct value into a standard curve equation to calculate the mycelium content of the soil. The method can achieve the purpose of rapidly detecting the content of Thelephora ganbajun mycelia in soil, and is simple and efficient.

The invention relates to a composite edible mushroom health-care food and a making method thereof. The composite edible mushroom health-care food consists of the following raw materials in parts by weight of 100-300 parts of black fungus powder, 60-140 parts of bolete powder, 50-250 parts of tricholoma quercicola zang powder, 30-70 parts of thelephora ganbajun zang powder, 200-400 parts of poria cocos powder and 150-350 parts of agricus bisporus powder. The composite edible mushroom health-care food not only can strengthen cell viability and improve the immunity, but also can achieve good conditioning effects on lobular hyperplasia of mammary gland, cystic hyperplasia or breast tumors. The composite edible mushroom health-care food is strict in making method, through repeated long-time sterilization, the storage of the composite edible mushroom health-care food is facilitated, and the quality guarantee period is prolonged.

The invention discloses a Sagittaria trifolia slice with a Thelephora ganbajun Zang taste and a preparation method thereof and belongs to the technical field of food processing. The Sagittaria trifolia slice solves the problem that the existing Sagittaria trifolia slice is prepared by frying and is bad for human health after long-term use. The Sagittaria trifolia slice is prepared from 80-90 parts by weight of Sagittaria trifolia starch, 20-30 parts by weight of Thelephora ganbajun Zang, 20-25 parts by weight of egg, 4-6 parts by weight of peanut oil, 2-3 parts by weight of table salt and 0.3-0.6 parts by weight of sodium bicarbonate. The preparation method comprises raw material selection, gelatinized starch preparation, baking raw material preparation, microwave baking and packaging. The Sagittaria trifolia slice is a non-fried food and is conducive to human health. The Sagittaria trifolia slice has a cool and refreshing taste of Sagittaria trifolia and a thick Thelephora ganbajun Zang fragrance. The Sagittaria trifolia and Thelephora ganbajun Zang produce synergism and have antioxidation effects. The Sagittaria trifolia slice is a good medicinal food.

The invention provides a specific DNA fragment for identification of thelephora ganbajun, an amplification primer, a preparation method and application thereof, and belongs to the technical field of biotechnology and enzyme engineering. A nucleotide sequence of the specific DNA fragment for the identification of the thelephora ganbajun is shown in an SEQ ID No.1 sequence table. The primer for amplifying the specific DNA fragment includes a 158F primer and a 437R primer. The specific DNA fragment for the identification of the thelephora ganbajun, the amplification primer or the application of the thelephora ganbajun specific DNA fragment obtained by the preparation method in the identification of the thelephora ganbajun can quickly and accurately complete the identification of the thelephora ganbajun.