The invention discloses a complex microbial agent used for ocean trash fish fermentation, and a preparation method for an enzymatic fish protein amino acid water-soluble fertilizer. The complex microbial agent consists of any three or four types of bacillus licheniformis, bacillus subtilis, bacillus megaterium, bacillus cereus, lactic streptococci and saccharomyces cerevisiae. The preparation method for the enzymatic fish protein amino acid water-soluble fertilizer comprises the following steps of: before ocean trash fishes are transported, the ocean trash fishes are subjected to pretreatmentthrough the complex microbial agent, breaking, enzymolysis, fermentation through the complex microbial agent, separation, filtering and concentration. The enzymatic fish protein amino acid water-soluble fertilizer is rich in various active substances and trace elements, including micromolecule polypeptides, amino acids, vitamins and the like, can meet the nutrition requirements of fruit and vegetable crops, improves the growth performance of fruit and vegetable crops as well as the quality of fruits and vegetables, reduces the use amount of chemical fertilizers, improves the quality of soil, reduces soil problems, including soil secondary salinization and the like, and has a good economic benefit.