The invention discloses Fructus aurantii disease-resistant high-yield cultivation technology. A culture medium is adopted for culture, so that absorption, on S-methyl benzoxazole [1, 2, 3] thiadiazole-7-benzothiodiazole, of seeds and seedlings can be improved effectively, resistance mechanism of Fructus aurantii can be activated efficiently, disease-resistance defense reaction of Fructus aurantii can be activated, Fructus aurantii strains with high disease resistance can be obtained, and disease probability of Fructus aurantii can be lowered. In addition, 10-13 parts of marigold stalk, 9-13 parts of perlite, 5-9 parts of vermiculite, 7-10 parts of Radix sophorae flavescentis and 8-13 parts of citrus peel residue which are weighed by weight are mixed to obtain a mixture, and 8-10 g of calcium superphosphate and 7-9 g of diammonium phosphate are added into each cube of the mixture for mixing to obtain a matrix, so that Fructus aurantii yield is increased and cultivation cost is lowered.