This invention relates to the field of food testing technology, and more particularly to a colorimetric LAMP method for the simultaneous
visual detection of
Vibrio vulnificus and
Vibrio alginolyticus. The method employs a LAMP detection
system composed of
DNA-functionalized
gold nanospheres and gold nanorods for detecting
Vibrio vulnificus and
Vibrio alginolyticus, respectively, and LAMP primer pairs. This allows for the detection and determination of the following states of
Vibrio vulnificus and
Vibrio alginolyticus: when neither
pathogen is present, the solution is transparent; when both pathogens are present, the solution is purplish-red; when only
Vibrio alginolyticus is present, the solution is gray; and when only
Vibrio vulnificus is present, the solution is purple. This method can accurately determine the presence or absence of
Vibrio vulnificus and Vibrio alginolyticus, and the specific type of
bacteria, avoiding misjudgments, missed detections, and inaccurate
traceability of these two types of
pathogen contamination in
food safety monitoring, clinical practice, and
aquaculture protection.