The invention discloses a KMnO4 colorimetric
information card sensor for rapidly detecting
xanthine oxidase in
liver cancer CTCs and application of the KMnO4 colorimetric
information card sensor. According to the method, the color of KMnO4 is gradually changed into pink, brown yellow and light yellow from
amaranth by utilizing the reduction effect of H2O2 on KMnO4,
visual detection of H2O2 is realized, the process can be completed within 1 minute, meanwhile, the change of an RGB
chromaticity space and an
absorbance value at the position of 526 nm can be caused, and an R / G value and an A526 value are in
linear correlation with the concentration of H2O2 within a certain range. XOD can selectively oxidize
xanthine (XA) to generate H2O2, KMnO4 is reduced in the presence of H2O2, the color of KMnO4 is changed from dark purple to dark brown and then to light yellow, and
visual detection of XOD and XA is achieved. By adjusting the concentration of the XOD or the XA, a detection method of the XOD can be constructed. Finally, the sensing method is applied to analysis of the concentration of XOD in
liver cancer CTCs, and it is found that the expression quantity is higher than that of normal cells. The method has the advantages of simplicity in operation, low cost,
visualization, high sensitivity and good specificity.