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Claret Chinese cabbage breeding method

A technology of purple and Chinese cabbage, applied in the field of cruciferous vegetable breeding, can solve the problem of no purple Chinese cabbage resources, and achieve the effect of rich varieties and high nutritional value

Inactive Publication Date: 2009-04-29
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are purple cabbage, laver sprouts, purple mustard greens, etc. among the Brassica vegetables, but there are no resources of purple Chinese cabbage.

Method used

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  • Claret Chinese cabbage breeding method
  • Claret Chinese cabbage breeding method
  • Claret Chinese cabbage breeding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043]Select the purple mustard local variety Hongyeqingcai from Qianyang, Hunan Province (quoted from the National Variety Resources Mid-term Repository of the Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, introduction number: II 3A0138), and selfed by optimizing continuous individual plants for 3-5 generations Select the mustard self-bred seeds with purple-red stems and leaves that have no trait separation from the offspring to obtain a genetically stable homozygous inbred line PM058.

[0044] Preparation of B5 extraction medium: According to the B5 medium described in the literature Gamborg, OL, et al., 1968, Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 151-158., first prepare a large number of elements Concentrated stock solution (concentrated 20 times), trace elements (concentrated 200 times), iron salt (concentrated 200 times), and organic substances other than sucrose (concentrated 200 times), then t...

Embodiment 2

[0053] Configure B5 solid medium: According to the B5 medium described in the literature Gamborg, OL, et al., 1968, Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res, 50, 151-158., first prepare a large number of elements Concentrated stock solution (concentrated 20 times), trace elements (concentrated 200 times), iron salt (concentrated 200 times), and organic substances other than sucrose (concentrated 200 times), then take a 1L volumetric flask, and take a large element concentrated stock solution 50ml, 5ml each of trace elements, iron salt and the above-mentioned organic substance concentrated stock solution, in addition, add the following substances to make the concentration of 6-benzylaminopurine (6-BA) 1.5mg·L -1 , Α-Naphthaleneacetic acid (NAA) 0.05mg·L -1 , Sucrose 30.0g·L -1 , Agar 7.0g·L -1 , Distilled water to a constant volume, then adjust the pH of the solution to 5.8-6.0, and dispense into 100ml Erlenmeyer flasks. After autoclaving (1...

Embodiment 3

[0065] F1 plants were obtained by using purple-red mustard PM058 as the female parent and Chinese cabbage DH line Orange Red Heart C24 as the male parent.

[0066] The obtained purple-red F1 plant was used as the female parent, and the Chinese cabbage DH line Orange Red Heart C24 was the male parent backcross to obtain (Orange Red Heart C24) BC1 seeds;

[0067] The obtained (Orange Red Heart C24) BC1 seeds were identified in the field, the purple-red plant was selected as the female parent, and the Chinese cabbage DH line Orange Red Heart C24 was used as the male parent to continue backcrossing to obtain (Orange Red Heart C24) BC2 seeds;

[0068] The obtained (Orange Red Heart C24) BC2 seeds were identified in the field, the purple-red plant was selected as the female parent, and the Chinese cabbage DH line Orange Red Heart C24 was used as the male parent to continue backcrossing. Through field selection and continuous backcrossing, normal flowering and seed setting were obtained....

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Abstract

The invention relates to the selection and cultivation method of purplish red cabbage. Plant generation F1 whose male parent is cabbage and female parent is purplish red rutabaga is cultivated by distant hybridization; the backcross of the hybrid plants takes cabbage as male parent; cabbage with stable hereditary performance and trait of purplish red is obtained by self-crossing purplish red plants whose general traits are similar to the traits of cabbage after generations of backcross. Different obvolvent types such as obvolvent , unrolling carpel, straight, overlapping-folded and conjunctionally folded can be obtained by the method according to the hereditary performance from male parent, meanwhile the growth season can be controlled in the same way. The invention enriches the breed variety of cabbage and leads a novel direction of breeding cabbage and improving the quality and nutritive value thereof.

Description

Technical field [0001] The invention relates to the technical field of cruciferous vegetable breeding, in particular to a method for breeding purple-red Chinese cabbage. Background technique [0002] Chinese cabbage (Brassica rapa L.ssp.pekinensis) is native to China. It is an annual and biannual herbaceous plant belonging to the genus Brassica of the cruciferous family. It includes two major groups of heading and non-heading. It is the most important vegetable in the genus Brassica. One of the crops, it is still the most widely distributed, the largest cultivated area, and the largest number of people in my country. [0003] With the continuous improvement of people's living standards, the quality breeding of Chinese cabbage has been paid more and more attention by many breeders. In recent years, orange-red Chinese cabbage and yellow-hearted Chinese cabbage, which have been bred successively at home and abroad, have been favored by consumers. Breeding of different ball colors ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04A01H1/02A01H5/12
Inventor 孙日飞李菲张淑江章时蕃
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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