Microbe preparation in use for treating high difficult wastewater and preparation method
A technology of microbial preparations and microbial strains, which is applied in the field of microbial preparations and its preparation, and can solve the problems of abnormal operation and high operating costs
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Embodiment 1
[0135] Embodiment 1: preparation is used to handle the microbial preparation of difficult waste water
[0136]After the activated sludge is screened and domesticated by microorganisms in the laboratory, the screened strains are mixed, and the anaerobic bacteria combination and the aerobic bacteria combination are matched in a ratio of 1:1, and the mixed bacteria are ensured The seed mixture contains 105 bacterial strains disclosed in the present invention, and then the mixed flora is divided into two halves, and aerobic fermentation culture and anaerobic fermentation culture are respectively carried out.
[0137] Among them, three kinds of culture medium are used for aerobic culture, which are respectively bacterial complete medium (such as beef extract peptone medium), glucose complete medium, and sucrose inorganic salt medium. The three kinds of medium are respectively inoculated and cultured, and the culture condition is 30 ° C. , 120 rev / min, and the time is 36 hours. Afte...
Embodiment 2
[0143] Embodiment 2: preparation is used to handle the microbial preparation of difficult waste water
[0144] The pH of the three mediums used in the aerobic culture was adjusted to 7.4 and sterilized at 121° C. for 15 minutes. The culture conditions of the aerobic culture were 32° C., 120 rpm, and the time was 24 hours. The culture temperature of the anaerobic culture is 32° C., stirring for 5 minutes every two hours, and the culture time is 48 hours. All the other preparation conditions and preparation steps are the same as in Example 1.
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