471 results about "Microbiologic agent" patented technology

The invention relates to the technical field of soil conditioners, and particularly discloses an intelligent controlled-release core-shell alginate soil conditioner and a preparation method thereof.The conditioner is of a core-shell-layer three-layer structure and comprises a core layer, a middle shell layer and an outer layer, the three layers account for 30%, 40% and 30% of the total weight respectively, and the core layer mainly comprises improved alginate and nutrients; the middle shell layer mainly comprises chitosan and a microbial preparation; and the outer layer mainly comprises modified charcoal and magnetic nano # imgabs0 #. The preparation method comprises the four steps of core layer preparation, middle shell layer coating, outer layer coating and forming post-treatment, and multiple functions of accurate nutrient supply, efficient heavy metal fixation and soil microenvironment regulation and control are achieved through a core-shell-layer three-layer structure design and a pH / temperature / microorganism triple response mechanism by adopting a coaxial electronic injection and layer-by-layer self-assembly technology; the fertilizer has a remarkable improvement effect on sandy soil and saline-alkali soil, and the organic matter increasing rate reaches 40%.

The invention relates to an agricultural microbial preparation production line multi-device collaborative control method and system, and the method comprises the steps: calculating a device health score based on operation state information, combining the device health score with a biological risk index to generate a task release score, and generating a production task instruction when the task release score meets a preset release threshold value, constructing a cooperative interlocking matrix between the multiple types of process equipment and the material handling equipment based on the production task instruction, distributing a control execution instruction to the multiple types of process equipment and the material handling equipment, respectively obtaining equipment feedback state information and material circulation information, executing consistency verification on the equipment feedback state information and the material circulation information, and sending a verification result to the material handling equipment; a consistency verification result is obtained, during manual modification, a revision audit chain corresponding to the control event information is generated, and when the running state information or the control event information triggers a preset abnormal threshold value, a disposal control instruction is generated. The method has the effect of improving the production safety.

The invention relates to the technical field of organic fertilizers, and discloses an agricultural waste two-stage fermentation bio-organic fertilizer and a preparation method thereof.The bio-organic fertilizer comprises 70%-80% of a mixture of livestock and poultry manure and straw subjected to anaerobic fermentation and aerobic fermentation two-stage treatment, 5%-10% of auxiliary organic matter, 3%-5% of a soil improvement material, 2%-4% of a functional additive and 1%-3% of a microbial preparation. The preparation method comprises the following steps: mixing the livestock and poultry manure and the straw according to a carbon-nitrogen ratio of 30: 1, and performing pretreatment; carrying out anaerobic fermentation for 7 days, and collecting biogas; performing solid-liquid separation to obtain a solid material and biogas slurry; concentrating the biogas slurry to prepare a leaf fertilizer; adding a microbial preparation into the solid material, and carrying out aerobic fermentation for 10-15 days; after fermentation is completed, drying, functional component adding, granulating, screening and packaging are conducted, a finished product is obtained, collaborative integration of anaerobic-aerobic fermentation and full-quantitative utilization of agricultural waste are achieved, and the method has the remarkable advantages of being short in fermentation period, small in nutrient loss, high in functionality and the like.

The invention discloses a paenibacillus kribbensis spaceflight mutant strain and application of the paenibacillus kribbensis spaceflight mutant strain in preparation of nano-selenium. According to the invention, a paenibacillus kribbensis PS04-2 strain is obtained through space mutation, and the strain has high selenite reducing capacity and can tolerate high-concentration selenite, and the tolerable concentration exceeds 1000mM; the method has the capacity of efficiently converting sodium selenite to generate nano-selenium, the synthesized nano-selenium is high in conversion rate and uniform in nano-selenium particle, and the provided preparation method is simple, convenient, low in cost, high in product purity and environmentally friendly. The nano-selenium synthesized by the PS04-2 strain has a good antagonistic effect on various plant pathogenic fungi, can prevent and treat various plant diseases, and can be used for developing more selenium-enriched microbial preparations. Meanwhile, nano-selenium synthesized by the PS04-2 strain is used for sesame production, so that sesame growth can be promoted, selenium-rich sesame can be obtained, and more effective methods are provided for increasing the selenium content of crops.

The invention relates to the technical field of microorganisms, in particular to application of bacillus glucans and a complex microbial inoculant thereof in preparation of bio-organic fertilizers, the strain Mh85 is separated from the root system of apple rootstock Malus hupehensis treated by a research group through trichoderma TB2, has good IAA production, nitrogen fixation, inorganic phosphorus decomposition and siderophore production capacities, and can be used for preparing bio-organic fertilizers. The strain Mh85 not only can directly promote plant growth, but also has a good growth-promoting effect on trichoderma TB2, and when the strain is compounded with the trichoderma TB2 to form a complex microbial inoculant, tomato seedling pot experiments are carried out. The complex microbial inoculant shows a synergistic effect, effectively overcomes the problems of unstable growth promoting effect, insufficient synergistic effect among strains and the like of an existing single microbial preparation, provides an efficient and stable technical scheme for improving plant growth, improving root development and increasing crop yield, and is a good raw material for preparing a bio-organic fertilizer.

The invention discloses a compound microorganism and application thereof to improvement of saline-alkali soil, and relates to the technical field of agriculture, the compound microorganism is obtained by compounding halophilic bacillus, rhodotorula mucilaginosa, alkali-resistant rhizobium, desulfurization vibrio and siliconizing bacteria, and the excellent saline-alkali soil improvement effect can be achieved. The functional strains cooperate with one another, have complementary advantages, and play a role in multiple aspects such as soil structure improvement, nutrient supply and absorption, salt and alkali relief, microbial community optimization and the like. The compound microbial preparation has an efficient synergistic mechanism, provides an innovative solution for solving the problem of saline-alkali soil, and shows huge potential in the aspects of promoting agricultural green development and ecological protection.

The invention provides a method for intensively removing antibiotic resistance genes in a composting process, and belongs to the technical field of solid waste treatment. The method comprises the following steps: performing aerobic composting on compost materials, and when the temperature in a compost body is reduced to 38-42 DEG C, inoculating a microbial preparation; the microbial preparation is a microbial agent with a function of promoting degradation of organic matters. Based on the principle that a microbial preparation is inoculated in a compost cooling period (38-42 DEG C), a microbial community structure is changed, and ARGs host bacterium proliferation is effectively inhibited, the effect of strengthening removal of resistance genes in the compost process is remarkable, operation is easy and convenient, control and implementation are easy, secondary pollution cannot be caused in the whole process, and the method is suitable for industrial production. The method has a wide prospect of popularization and application in a large range.

The invention relates to a microbacterium ester, a microbial preparation thereof and a method for degrading plastics by using the microbacterium ester, the strain is microbacterium ester 2A3-D1, the microbacterium ester is registered in China General Microbiological Culture Collection Center on June 19, 2025, and the preservation number is CGMCC No.34950. The invention further discloses a method for degrading plastics by using the microbacterium ester. The invention relates to a microbial preparation containing microbacterium esterotorum 2A3-D1 as an active component. The invention relates to a method for degrading polyethylene and polypropylene micro-plastics by utilizing microbacterium esteroflavus 2A3-D1. The strain can grow by taking polyethylene and polypropylene micro-plastics as a unique carbon source, and has the capability of degrading the polyethylene and polypropylene micro-plastics. The method is applied to degradation of polyethylene and polypropylene plastics in a river in-situ environment, and has a wide application prospect.

The invention belongs to the field of microbial preparations, and particularly relates to a complex microbial inoculant for promoting microbial quorum sensing and application of the complex microbial inoculant in compost. The composite microbial agent comprises pseudomonas A3 and paenibacillus polymyxa 21-2, and the preservation number of the pseudomonas A3 is CCTCC (China Center for Type Culture Collection) NO: M20251394. The pseudomonas A3 can express key genes such as rhlI and lasI of a gram-negative bacteria AHL quorum sensing system and signal molecules of the key genes, and the paenibacillus polymyxa 21-2 can express luxS genes of a gram-positive bacteria AI-2 signal system and signal molecules DPD of the luxS genes. In the pig manure compost, the complex microbial inoculant is added, so that the expression of AI-2 and AHLs system related genes in the compost and the content of signal molecules DPD are remarkably improved, the microbial population sensing process of the compost is promoted, finally, the compost temperature is increased by about 6 DEG C, the compost carbon-nitrogen ratio is reduced, and the compost maturity is promoted.

The invention provides application of candida tropicalis and a preparation thereof in prevention and treatment of tobacco mildew, and belongs to the technical field of microorganisms. A strain of candida tropicalis is named as candida tropicalis 2-17, the preservation number of the candida tropicalis 2-17 is CCTCC NO: M 20242184, a microbial preparation prepared from the candida tropicalis can inhibit growth of main mould in tobacco leaves, and the candida tropicalis has good application potential for biological prevention and treatment of tobacco leaf mould.

The invention relates to the technical field of microorganisms, in particular to animal-derived pediococcus acidilactici and application thereof. The pediococcus acidilactici is classified and named as pediococcus acidilactici, the strain number of the pediococcus acidilactici is 12NN1, the pediococcus acidilactici is preserved in Guangdong Microbial Culture Collection Center on January 2, 2025, and the preservation number of the pediococcus acidilactici is GDMCC No: 65706. The strain is resistant to acid and cholate, has a remarkable inhibiting effect on growth and reproduction of pathogenic escherichia coli and staphylococcus aureus, is safe and non-toxic, does not have pathogenicity, and provides beneficial strain resources and biological materials for development of corresponding microbial preparations. Therefore, the compound can be used for preparing a biocontrol preparation for inhibiting the growth of pathogenic bacteria, preparing animal feed and preparing a preparation for preventing, improving and / or treating gastrointestinal tract pathogenic bacteria infection, so that the compound has a wide application prospect.

The invention discloses enterobacter hormaechei XW-01 and a microbial preparation thereof, the strain (preservation number is CCTCC NO: M 20242595) can efficiently degrade protein, total sugar and nicotine in tobacco leaves, sensory indexes such as aroma and aroma quantity are remarkably improved, and protease activity is remarkably improved when the enterobacter hormaechei XW-01 and wheat bran act synergistically. The microbial preparation is obtained through the processes of strain activation, fermentation culture and microbial agent preparation, and when the microbial preparation is applied to tobacco leaf treatment, through the steps of water content adjustment, preparation spraying, sealed fermentation, low-temperature drying and the like, the technical breakthrough that the protein content is reduced, the tar release amount is reduced, and the comprehensive quality grade is improved is achieved. Compared with a traditional method, the technology has the advantages that the treatment period is shortened, the cost is greatly reduced, the COD value of wastewater is greatly reduced, the cigarette quality and the production environmental protection property are remarkably improved, and the method has a wide industrial application prospect.

The invention discloses a composite microbial preparation for treating obesity of pet dogs and application of the composite microbial preparation, and belongs to the technical field of microbial preparations. The compound microbial preparation is prepared by fermenting lactobacillus acidophilus L100 and lactobacillus reuteri GBW1146, and the preservation number of the lactobacillus reuteri GBW1146 is CGMCC (China General Microbiological Culture Collection Center) No. 34455. The compound microbial preparation is prepared by fermenting lactobacillus acidophilus L100 and lactobacillus reuteri GBW1146. The compound microbial preparation can remarkably reduce digestion and absorption of intestinal tracts of dogs on fat and reduce triglyceride and cholesterol levels in blood, so that the average food intake and average calorie consumption of the dogs are reduced, and finally the effect of reducing obesity of the pets is achieved. The compound microbial preparation provided by the invention enhances the competitiveness of the pet feed additive and series products thereof in China in the international market, and has a wide application prospect.

The invention discloses a microbiological deodorization method, which belongs to the technical field of disinfection and deodorization, and comprises the following steps: S1, preparing a microbial preparation, S2, pre-stirring a strain combination; S3, preparing a deodorant from the prepared composite microbial preparation according to the mass; and S4, putting a prepared deodorant into an ultrasonic atomizer or a spray tower. According to the invention, the microbial preparation is creatively adopted to perform spray deodorization on the odor source, so that the deodorization efficiency is high, the effect is good, the treatment cost of the odor source and odor is effectively reduced, the overall operation difficulty is low, only the polluted area is atomized, the odor is effectively controlled before volatilization, the odor pollution spreading can be effectively controlled, and the influence on the operation environment is avoided; the microbial inoculum is non-toxic, can be naturally degraded, has no residue after deodorization, does not need additional neutralization and filtration, has higher environmental adaptability, and meets the deodorization requirements of small and medium-sized farms and small and medium-sized garbage collection stations.

The invention discloses a dual-purpose composite microbial preparation for straw comprehensive treatment and a straw fermentation method.According to the preparation, a composite microbial system is formed by a core bacterial group and an auxiliary bacterial group, the adaptability to the environment temperature is high, and the preparation can be applied to high-temperature composting or straw returning as required; the preparation utilizes the comprehensive decomposition effect of microorganisms and functional enzymes to destroy the compact cellulose structure on the surface layer of the straw, so that the sugar and nutrients in the straw are released, the composting decomposition, field decomposition and the like of the straw are efficiently promoted, the problems of low decomposition speed, difficulty in temperature control and the like in the straw treatment process are effectively solved, and the quality of the straw is improved. Therefore, efficient and green treatment and resource utilization of the straws are realized, and meanwhile, a new technical path is provided for green and environment-friendly treatment of agricultural wastes.

The invention relates to the technical field of microorganisms, in particular to enterococcus faecalis EF-1 capable of reducing blood lipid, a microbial preparation and application of the enterococcus faecalis EF-1. The enterococcus faecalis EF-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on September 27, 2024, and the preservation number is CGMCC No.32097. The enterococcus faecalis EF-1 is named as enterococcus faecalis EF-1. The enterococcus faecalis EF-1 has the functions of: (1) relieving the weight increase speed of animals, reducing body weight gain, and preventing, relieving or treating obesity; (2) the contents of triglyceride, cholesterol and low-density lipoprotein cholesterol in liver and serum are reduced; (3) the liver function is protected, and the contents of ALT and AST in serum and liver are reduced; (4) liver lipid deposition and lesion are relieved; (5) relieving adipose tissue hypertrophy; (6) preventing, relieving or treating metabolic syndrome; (7) reducing blood sugar, improving insulin resistance and relieving diabetes mellitus; 8, increasing the content of short-chain fatty acid; and (9) regulating intestinal flora.

The invention discloses bacillus subtilis MY-01 as well as a microbial preparation and application of the bacillus subtilis MY-01. The strain (preservation number is CCTCC NO: M 20242596) can efficiently degrade cellulose, total sugar and nicotine in tobacco stems and remarkably improve sensory indexes such as aroma quality and aroma amount, and the cellulase activity is greatly improved when the strain and sodium carboxymethyl cellulose act synergistically. The microbial preparation is obtained through the processes of strain activation, fermentation culture and microbial agent preparation, and when the microbial preparation is applied to tobacco stem treatment, the technical breakthrough of reduction of the cellulose content, increase of the filling value and reduction of the tar release amount is achieved through the steps of water content adjustment, preparation spraying, sealed fermentation, low-temperature drying and the like. Compared with a traditional method, the technology has the advantages that the treatment period is greatly shortened, the cost is remarkably reduced, the COD value of wastewater is reduced, the cigarette quality and the production environmental protection property are remarkably improved, and the industrial application prospect is wide.

The invention discloses a salt-tolerant brevibacillus for promoting growth and preventing and controlling plant diseases and application of the salt-tolerant brevibacillus. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025, and the preservation number of the brevibacillus halotolerans T101 is GDMCC No: 66731. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025. The strain T101 disclosed by the invention can effectively antagonize plant pathogenic fungi peronophythora litchii and plant pathogenic bacteria such as pseudomonas syringae, xanthomonas campestris, citrus canker pathogen and ralstonia solanacearum, can generate siderophores and plant growth hormone IAA, and can remarkably promote the growth of tomato root systems in a pot experiment. Therefore, the strain T101 can be used for preparing microbial preparation products for preventing and treating plant diseases and promoting plant root growth, and has important application potential in the aspects of microbial agents, biological fertilizers and the like for promoting plant growth and development.

The invention relates to bacillus megatherium XW-02 (the preservation number is CCTCC NO: M 2025185), a microbial preparation of the bacillus megatherium XW-02, and application of the microbial preparation. The bacillus megatherium XW-02 has the advantages that the preservation number is CCTCC NO: M 2025185; the strain can efficiently express xylanase through xylan induction, hemicellulose in tobacco leaves is remarkably degraded, chemical composition is optimized, and combustion harmful substances are reduced. The treated cigar has the advantages that the aroma richness is improved, the purity is improved, the taste mellowness is improved, and the overall quality is improved by 1-2 grades. The xylan extracted from agricultural wastes is used as an inducer to construct a green biological manufacturing system, the cost is reduced by more than 80% compared with the traditional process, and the emission of three wastes is reduced. The process compatibility is high, the method can be matched with an existing production line only by additionally arranging a strain culture module, the single-line annual tobacco leaf treatment amount reaches 500 tons, and economic benefits are remarkable. The invention provides a safe, efficient and environment-friendly quality improvement solution for the tobacco industry, and has great industrialization value.

The invention provides a bifidobacterium longum subsp. Longum aFMT-103 strain, which is classified and named as Bifidobacterium longum subsp. Longum, is preserved in Guangdong Microbial Culture Collection Center, and has a preservation number of GDMCC No: 65181. The invention also provides a preparation method of the bifidobacterium longum subsp. Longum aFMT-103 strain. The invention further provides a microbial preparation containing the aFMT-103 bacterial strain. In addition, the invention also provides an application of the strain or the microbial preparation in preparation of an anti-depression product, and also provides an anti-depression product. The bifidobacterium longum subsp. Longum aFMT-103 strain has the advantages that the bifidobacterium longum subsp. Longum aFMT-103 strain is separated from the faeces of healthy adults; the strain has a remarkable anti-depression effect, can be used for preparing a preparation for resisting depression symptoms, and is of great significance in prevention and adjuvant therapy of depression.

The bacillus velezensis MY-8 disclosed by the invention has obvious broad-spectrum antibacterial activity, and has an obvious inhibition effect on various plant pathogenic bacteria including cabbage alternaria alternata, rhizopus arrhizus, cladosporium very fine, fusarium tripteroides, alternaria alternata, Columbia cladosporium, botryosphaeria dothidea, fusarium oxysporum, geotrichum candidum, colletotrichum gloeosporioides and A. arundinis, and the bacillus velezensis MY-8 has an obvious inhibition effect on various plant pathogenic bacteria such as bacillus velezensis MY-8. The compound has a remarkable control effect on downy mildew, is expected to be prepared into a microbial preparation for biological control, and can be used as a biopesticide, a bio-fertilizer, a plant growth promoter and the like. The bacillus velezensis MY-8 disclosed by the invention can also express a structural domain containing protein of DUF4393, so that the bacillus velezensis MY-8 has potential bacteriophage infection resistance.

The invention discloses a sewage treatment system which is composed of a grating pretreatment unit, an oil separation tank, a high-speed air flotation machine, a coagulation primary sedimentation tank, a hydrolytic acidification and anoxic tank, a screening nano bubble generator, a contact oxidation and aerobic tank of microbial filler, a microbial preparation coagulation sedimentation unit, a sludge treatment unit, a sulfur-autotrophic phosphorus and nitrogen removal unit, an ultraviolet disinfection unit and the like. The air dissolving efficiency of the micro-charge high-speed air flotation machine is as high as 95-99%, the equipment volume is only 1 / 5 of that of traditional equipment, and the decontamination and separation effects are good; the water outlet and air outlet amount of the screening nano bubble generator is 10 times larger than that of a conventional nano aerator, ionized water, small molecular group water and magnetized water are generated in a combined mode, so that charges are carried, the solubility and the permeability are high, microorganisms are activated, the biochemical rate is increased, pollutants are effectively removed, sludge is degraded, and the sludge yield is reduced. The coagulation of the microbial preparation enables the inclined tube precipitation effect to be better, and the sulfur autotrophic technology and the efficient denitrification of the denitrification filter material solve the problems of insufficient carbon sources and denitrification, so that the sewage treatment constantly reaches the standard.

The invention relates to the field of microorganisms, in particular to lactobacillus plantarum TRT-01 with anti-inflammatory and bacteriostatic functions as well as a preparation method and application of the lactobacillus plantarum TRT-01, the lactobacillus plantarum TRT-01 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32237, and the preservation date is October 17, 2024. The plant lactobacillus has broad-spectrum bacteriostatic activity on 10 pathogenic bacteria such as helicobacter pylori, streptococcus mutans and the like, and can regulate Th1 / Treg immune balance and relieve inflammatory response by inducing dendritic cells to secrete cell factors IL-10 and IL-12, inducing T cells to secrete cell factors TGF-beta and IFN-gamma and inhibiting secretion of cell factors IL-6 at the same time. Therefore, the plant lactobacillus TRT-01 disclosed by the invention can be used for preparing a composition with anti-inflammatory and bacteriostatic effects, is suitable for the fields of medicines, foods, health-care products and the like, and is particularly suitable for developing a microbial preparation with a probiotic effect.

The invention belongs to the field of functional composite microbial preparations, and particularly discloses a microbial agent and GABA compounded composition and application thereof in promoting growth and development of children. The composition is prepared from a freeze-dried bacterial agent and a GABA (gamma-aminobutyric acid) compound, wherein the freeze-dried bacterial agent comprises lactobacillus johnsonii YH1136 and lactobacillus helveticus Helveticus; the GABA compound is prepared by loading GABA with the purity of greater than or equal to 98% on a nano cellulose carrier, and the loading rate is 20-35%. The composition realizes high survival rate of the microbial inoculum and targeted slow release of GABA through a freeze-drying process and enteric coating. Experiments show that the composition can significantly improve the serum GH / IGF-1 level, the bone mineral density and the body growth rate, and has a synergistic effect when combined with the vitamin D3. According to the invention, the problems of single strain function, low GABA utilization rate and gastric acid damage in the prior art are solved, and an efficient and safe solution is provided for children growth and development intervention.

The invention relates to an application of a compound microbial agent in promoting phosphorus and potassium solubilization of soil and increasing the content of available phosphorus and potassium in the soil. The bacterial strains in the compound microbial agent consist of a bacillus coagulans BC66 bacterial strain, a lactobacillus plantarum LP194 bacterial strain and a lactobacillus acidophilus LA71 bacterial strain. The invention also relates to an application of the compound microbial agent in promoting phosphorus and potassium solubilization of the soil and increasing the content of available phosphorus and potassium in the soil, and the bacterial strains in the compound microbial agent consist of a bacillus coagulans BC66 bacterial strain, a lactobacillus plantarum LP194 bacterial strain and a lactobacillus acidophilus LA71 bacterial strain. The invention develops a brand-new microbial preparation capable of promoting the phosphorus and potassium solubilizing effect of soil, and researches find that the three strains are mutually matched and have synergistic interaction on the effects of promoting phosphorus and potassium solubilizing of soil, improving the content of available phosphorus and potassium in soil, improving the utilization rate of phosphate fertilizer and potassium fertilizer and increasing the yield of fruit trees, and the yield of the fruit trees is increased under the condition that the using bacterium amount is consistent. Compared with a single strain intervention mode or other strain combined intervention mode, the microbial preparation has the advantage that the effects are obviously improved. Therefore, the compound microbial agent provides a new strategy for promoting phosphorus and potassium solubilization of soil, increasing the content of available phosphorus and potassium in soil, increasing the utilization rate of phosphate fertilizer and potassium fertilizer and increasing the yield of fruit trees.

The present disclosure relates to the field of cosmetics, and particularly to Bifidobacterium longum subsp. infantis, a microbial agent and use thereof. The present disclosure provides Bifidobacterium longum subsp. infantis, with preservation No. CGMCC NO. 27851. A species related to in the present disclosure can achieve efficacies of repairing, anti-inflammation, whitening and the like in food and beauty fields, has a wider range of efficacies, and can play a better role in the field of beauty and skin care.

The invention belongs to the field of microbial preparations, and discloses a microbial stabilizer which comprises non-bacillus or a zymophyte solution thereof and a chemical stabilizer, and the non-bacillus comprises at least one of serratia nematophila and acinetobacter calcoaceticus. The invention also discloses a preparation method of the microbial stabilizer and an application of the microbial stabilizer in prolonging the shelf life of a microbial agent. The research on the microbial stabilizer containing non-bacillus discovers that the microbial stabilizer formed by proper matching of the non-bacillus and the chemical stabilizer can keep the stability of the concentration of viable bacteria for a long time, prolong the shelf life of microbial products and reduce the requirements of frequent production and storage; therefore, the production cost is directly reduced, the economic benefit is improved, and the method can be used in the technical field of microbial preparations.

The invention relates to the technical field of microorganisms, and particularly discloses a microbial preparation for improving soil-borne diseases of soil and a preparation method thereof.The microbial preparation is prepared from modified biochar, a compound microbial agent, plant essential oil, chitosan and konjaku flour; the preparation method comprises the following steps: soaking the modified charcoal in the compound microbial agent, adding the konjaku flour, the chitosan and the plant essential oil, uniformly stirring and mixing, and then freeze-drying to obtain the required microbial preparation for improving the soil-borne diseases of the soil. The microbial preparation prepared by the invention has the beneficial effects of promoting plant root growth and improving soil-borne diseases.

The invention discloses a process parameter regulation and control method and system for the real-time fermentation state of an agricultural microbial preparation, and the method comprises the steps: S1, building a monitoring and data integration platform, carrying out the coding management of a fermentation container, collecting multi-dimensional data, carrying out the standardization processing, and building a historical database; s2, developing a visual display system, presenting fermentation room layout and color block superposition duration information in a graphical interface, and early warning deviation; s3, constructing an evaluation and prediction model, evaluating the state through a deep neural network and a time sequence algorithm, and predicting the progress; s4, developing an adjustment strategy generation engine, automatically analyzing an abnormal reason and generating a multi-parameter collaborative optimization suggestion; and S5, evaluating the regulation and control effect and continuously optimizing. The system comprises a monitoring and data integration module, a visual display module, an evaluation and prediction module, an adjustment strategy generation module and an effect evaluation optimization module. Intelligent upgrading of the whole production process is achieved, the response speed and decision accuracy are improved, the fermentation regulation lag loss is reduced, and high-quality production is guaranteed.

The invention provides compound bacteria for biological enhancement and sludge reduction and a preparation method of the compound bacteria. The compound microbial preparation comprises 25 microbial strains capable of widely degrading organic compounds and sludge zoogloea, original strains with specific degradation capability are subjected to multi-step pure culture fermentation under limited conditions and then are compounded to obtain the compound microbial agent. The composite microbial preparation can be used for degrading various organic matters including aromatic hydrocarbons, alcohols, aldehydes, heterocyclic rings, halogenated hydrocarbons, anilines, phenols and the like and aged activated sludge zoogloea, and can be used for synergism and sludge in-situ reduction of biological systems of various industrial wastewater including municipal wastewater, petrochemical wastewater, synthetic chemistry, printing and dyeing wastewater and the like. Due to the excellent extracellular hydrolase producing function of the compound bacteria, the sludge yield of an existing biochemical system can be remarkably reduced, and in-situ reduction of the sludge is achieved.