267 results about "Microbiologic agent" patented technology

The invention relates to a microbacterium ester, a microbial preparation thereof and a method for degrading plastics by using the microbacterium ester, the strain is microbacterium ester 2A3-D1, the microbacterium ester is registered in China General Microbiological Culture Collection Center on June 19, 2025, and the preservation number is CGMCC No.34950. The invention further discloses a method for degrading plastics by using the microbacterium ester. The invention relates to a microbial preparation containing microbacterium esterotorum 2A3-D1 as an active component. The invention relates to a method for degrading polyethylene and polypropylene micro-plastics by utilizing microbacterium esteroflavus 2A3-D1. The strain can grow by taking polyethylene and polypropylene micro-plastics as a unique carbon source, and has the capability of degrading the polyethylene and polypropylene micro-plastics. The method is applied to degradation of polyethylene and polypropylene plastics in a river in-situ environment, and has a wide application prospect.

The invention discloses a microbiological deodorization method, which belongs to the technical field of disinfection and deodorization, and comprises the following steps: S1, preparing a microbial preparation, S2, pre-stirring a strain combination; S3, preparing a deodorant from the prepared composite microbial preparation according to the mass; and S4, putting a prepared deodorant into an ultrasonic atomizer or a spray tower. According to the invention, the microbial preparation is creatively adopted to perform spray deodorization on the odor source, so that the deodorization efficiency is high, the effect is good, the treatment cost of the odor source and odor is effectively reduced, the overall operation difficulty is low, only the polluted area is atomized, the odor is effectively controlled before volatilization, the odor pollution spreading can be effectively controlled, and the influence on the operation environment is avoided; the microbial inoculum is non-toxic, can be naturally degraded, has no residue after deodorization, does not need additional neutralization and filtration, has higher environmental adaptability, and meets the deodorization requirements of small and medium-sized farms and small and medium-sized garbage collection stations.

The invention discloses a salt-tolerant brevibacillus for promoting growth and preventing and controlling plant diseases and application of the salt-tolerant brevibacillus. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025, and the preservation number of the brevibacillus halotolerans T101 is GDMCC No: 66731. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025. The strain T101 disclosed by the invention can effectively antagonize plant pathogenic fungi peronophythora litchii and plant pathogenic bacteria such as pseudomonas syringae, xanthomonas campestris, citrus canker pathogen and ralstonia solanacearum, can generate siderophores and plant growth hormone IAA, and can remarkably promote the growth of tomato root systems in a pot experiment. Therefore, the strain T101 can be used for preparing microbial preparation products for preventing and treating plant diseases and promoting plant root growth, and has important application potential in the aspects of microbial agents, biological fertilizers and the like for promoting plant growth and development.

The invention discloses a process parameter regulation and control method and system for the real-time fermentation state of an agricultural microbial preparation, and the method comprises the steps: S1, building a monitoring and data integration platform, carrying out the coding management of a fermentation container, collecting multi-dimensional data, carrying out the standardization processing, and building a historical database; s2, developing a visual display system, presenting fermentation room layout and color block superposition duration information in a graphical interface, and early warning deviation; s3, constructing an evaluation and prediction model, evaluating the state through a deep neural network and a time sequence algorithm, and predicting the progress; s4, developing an adjustment strategy generation engine, automatically analyzing an abnormal reason and generating a multi-parameter collaborative optimization suggestion; and S5, evaluating the regulation and control effect and continuously optimizing. The system comprises a monitoring and data integration module, a visual display module, an evaluation and prediction module, an adjustment strategy generation module and an effect evaluation optimization module. Intelligent upgrading of the whole production process is achieved, the response speed and decision accuracy are improved, the fermentation regulation lag loss is reduced, and high-quality production is guaranteed.

The invention relates to the technical field of microorganisms, and particularly discloses a lactobacillus paracasei KQNIU, a microbial preparation and application of the lactobacillus paracasei KQNIU. The Lactobacillus paracasei KQNIU is preserved in the China General Microbiological Culture Collection Center on August 25, 2025, the preservation number is CGMCC No.35739, and the Lactobacillus paracasei KQNIU is classified and named as Lactobacillus paracasei, and the Lactobacillus paracasei KQNIU is named as Lactobacillus paracasei KQNIU. The Lactobacillus paracasei KQNIU is named as Lactobacillus paracasei KQNIU. The lactobacillus paracasei KQNIU provided by the invention has good gastrointestinal tract tolerance, has an inhibition effect on pathogenic bacteria such as escherichia coli, salmonella and the like, can be prepared into a microbial preparation or a feed additive for preventing and treating calf diarrhea, replaces antibiotics and realizes green and healthy breeding.

The invention provides a stenotrophomonas sp. CLX-022 strain which has a good antagonistic effect on colletotrichum gloeosporioides and fusarium fusiforme, and the strain is preserved in China Center for Type Culture Collection (address: Wuhan University, Wuhan, China) and has a good antagonistic effect on colletotrichum gloeosporioides and fusarium fusiforme. The strain is preserved in China Center for Type Culture Collection (CCTCC) NO: M20252110 on September 24, 2025, the postal code is 430072, and the preservation number is CCTCC NO: M20252110. The 16S rDNA (ribosomal deoxyribonucleic acid) sequence of the strain is as shown in SEQ ID NO. 1. The stenotrophomonas CLX-022 strain is high in antibacterial property and can be used for preparing a microbial preparation for treating / preventing strawberry anthracnose and rice bakanae disease.

The invention relates to the technical field of microbial preparations, and particularly discloses bacillus velezensis HY16 fermentation liquor freeze-dried powder and a preparation method and application thereof.The preparation method includes the steps that hydroxyl of beta-cyclodextrin reacts with anhydride of maleic anhydride, double bonds are introduced to beta-cyclodextrin molecules, and then under the action of persulfate serving as an initiator, the beta-cyclodextrin and maleic anhydride react with each other to prepare beta-cyclodextrin HY16 fermentation liquor freeze-dried powder; and carrying out free radical copolymerization reaction on the beta-cyclodextrin and 2-acrylamide-2-methylpropanesulfonic acid to obtain the modified beta-cyclodextrin. Sulfonic acid groups in 2-acrylamide-2-methylpropanesulfonic acid are strong hydrophilic groups, the water solubility of beta-cyclodextrin can be remarkably improved, growth of ice crystals during freezing is delayed, mechanical damage of the ice crystals to strains is reduced, and meanwhile negative charges of the sulfonic acid groups can be effectively absorbed through electrostatic repulsion. The freeze-drying process is simple, aggregation and inactivation of fermentation active substances or strains in the freeze-drying process are prevented, the survival rate of viable bacteria in the freeze-drying powder is increased, the stability of active ingredients in fermentation liquor is kept, and then the algae-lysing effect is improved.

The application relates to the technical field of soil remediation, and particularly discloses a composite soil remediation agent, a preparation method and application thereof.A composite soil remediation agent comprises modified calcined oyster shell, plant biomass carbon, bone charcoal, compost, microbial preparation and ferrous sulfide; the preparation of the modified calcined oyster shell comprises the following steps: crushing, calcining oyster shell, and then adding a modified solution prepared from urea, rhamnolipid, iminodisuccinic acid tetrasodium and sodium dodecylbenzenesulfonate to modify the calcined oyster shell, so that the modified calcined oyster shell is obtained.The composite soil remediation agent can effectively adsorb and fix heavy metals in slightly heavy metal contaminated soil, reduce the mobility and bioavailability of the heavy metals, and thus reduce the harm of the heavy metals to soil and crops.

Disclosed herein are methods, systems and devices for screening compounds for efficacy against biofilm formation. These methods have the advantage over the prior art, in that the biofilm formation (or inhibition thereof) is tested directly on the material of interest, thereby providing a high throughput system for testing antimicrobial formulations against corrosion. Also disclosed is a peg assembly, and kits comprising a peg assembly, wherein the pegs are composed of a material of interest.

The invention discloses serratia marcescens R-Do03 and application of the serratia marcescens R-Do03 in preparation of a microbial preparation for preventing and treating black spot of Chinese yam, the serratia marcescens R-Do03 is preserved in China General Microbiological Culture Collection Center, the preservation date is September 17, 2025, and the preservation number is CGMCC No.35939. The serratia marcescens R-Do03 can be used for preventing and treating black spot of Chinese yam. The invention also specifically discloses an application of the serratia marcescens R-Do03 in preparation of a microbial preparation for preventing and treating black spot of Chinese yam. The serratia marcescens R-Do03 strain fermentation liquor screened by the invention can remarkably inhibit the growth of pathogenic bacteria of the Chinese yam black spot, and the extract of the fermentation liquor can effectively inhibit the pathogenicity of the pathogenic bacteria, so that the serratia marcescens R-Do03 has a remarkable prevention and control effect on the Chinese yam black spot, is expected to be developed as a novel biocontrol agent, and has a wide application prospect. And the method has a wide application prospect for industrial development of Chinese yam.

The present application relates to a method and system for collaborative management of multiple devices in an agricultural microbial preparation production line, which method comprises calculating a device health score based on operating state information, combining the device health score with a biological risk index to generate a task release score, generating a production task instruction when the task release score meets a preset release threshold, constructing a collaborative interlocking matrix between multiple types of process equipment and material handling equipment based on the production task instruction, distributing control execution instructions to the multiple types of process equipment and material handling equipment, obtaining device feedback state information and material flow information respectively, performing consistency verification on the device feedback state information and the material flow information to obtain a consistency verification result, generating a revision audit chain corresponding to the control event information when manually modified, and generating a disposal control instruction when the operating state information or the control event information triggers a preset abnormal threshold. The present application has the effect of improving production safety.

The invention discloses a preparation method of a microbial preparation for preventing and treating powdery mildew of melons, which comprises the following steps: firstly preparing keratin microspheres, modifying the keratin microspheres with acryloylmorpholine, and compounding the modified keratin microspheres with mesoporous silica and an ethylene-vinyl alcohol copolymer to obtain a composite carrier; and finally, immersing the carrier into a mixed bacteria solution of ceriporia lacerata, bacillus velezensis and bacillus agave for adsorption and drying. Stable immobilization of strains is achieved through multiple interactions, a protective film can be formed on the surfaces of leaves through illumination after the preparation is sprayed, pathogenic bacteria are physically prevented from being attached and infected, and the fungicide can be intelligently released along with the change of environment humidity; the three strains have a synergistic effect to efficiently prevent and control powdery mildew pathogenic bacteria of melon crops by preempting sites, degrading pathogenic bacteria structures, secreting antibacterial substances and inducing plant immunity, meanwhile, the utilization efficiency and quality of crop nutrients are improved, and the application prospect is good.

The application discloses a plant lactobacillus strain and application thereof, and belongs to the technical field of microorganisms. The preservation number of the strain is CGMCC No. 33864. The strain has good tolerance to gastrointestinal juice and bile salts and has no hemolytic activity. Concentrated cell-free supernatant prepared from the strain can inhibit salmonella, including inhibiting the growth of salmonella, killing bacteria, inhibiting or removing biofilm. Animal experiments show that the strain and the concentrated cell-free supernatant thereof can prevent salmonella infection, alleviate weight loss caused by infection, maintain intestinal barrier integrity, reduce the level of pro-inflammatory factors, alleviate tissue damage, improve the expression of colon tight junction proteins and mucin, promote the generation of short-chain fatty acids, and restore the intestinal flora structure. The application further provides a microbial preparation containing the strain and / or the concentrated cell-free supernatant thereof. The strain and the microbial preparation can be used for preparing products for resisting salmonella, preventing salmonella infection or improving intestinal health.

The application discloses a floating composite microbial preparation for removing marine oil pollution and a preparation method thereof. The floating composite microbial preparation contains bacillus thuringiensis NH26 strain, hollow glass microbeads and a lipopeptide surfactant purified from a fermentation liquor of the NH26 strain, has floating characteristics, can rapidly activate and efficiently degrade oil hydrocarbon, has good degradation capacity for alkanes and polycyclic aromatic hydrocarbons in seawater, and still has good degradation capacity for alkanes in seawater containing high-concentration oil hydrocarbon. The floating composite microbial preparation can be used for removing marine oil pollution and repairing marine environment. In addition, the application further provides a preparation method of the floating composite microbial preparation. The preparation method has simple preparation process, the obtained floating composite microbial preparation has uniform particle size, has high embedding rates for nitrogen and phosphorus, and can be prepared on a large scale.

The invention discloses an aquaculture wastewater treatment device adopting a microbiological treatment technology, and relates to the technical field related to aquaculture wastewater treatment.The aquaculture wastewater treatment device comprises a gantry moving frame, a liquid supply pipe and an exchange assembly, and the liquid supply pipe is filled with microbial preparation liquid through a liquid supply mechanism; the liquid feeding pipe is provided with a plurality of spraying nozzles, the spraying nozzles are used for pushing and discharging microbial preparation liquid out of the liquid feeding pipe, the spraying nozzle disc is used for spraying the microbial preparation liquid and spraying and putting aquaculture wastewater on the surface, the exchange assembly is used for switching a moving state or a static state between the liquid feeding pipe and the spraying nozzle disc, and the spraying nozzle disc moves up and down on the liquid feeding pipe. Deeply spraying and putting the aquaculture wastewater. According to the breeding wastewater treatment device adopting the microbiological treatment technology, free conversion between deep-layer spraying and feeding and surface spraying and feeding of microbial preparation liquid is achieved, the diversity of feeding modes is guaranteed, and in addition, during deep-layer spraying and feeding, soft stirring treatment is achieved.

The invention discloses an application of trichoderma griseaginii TR2122 in prevention and treatment of beet root rot, a strain of the trichoderma griseaginii TR2122 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.21025, and the strain has strong antagonism and hyperparasitism effects on fusarium oxysporum causing the beet root rot. Meanwhile, the invention further provides a microbial preparation containing the live spores of the strain, the spore content is not lower than 2 * 10 < 8 > CFU / g, and the microbial preparation is preferably wettable powder containing a parent drug, a dispersing agent, a wetting agent and filler. In addition, the invention further discloses a preparation method of the preparation, and the preparation method comprises the steps of fermentation culture, concentration drying and mixing preparation. The strain and the preparation have stable and lasting control effect on beet root rot, are superior to or equivalent to conventional chemical agents, are safe and environment-friendly, and provide an effective solution for beet green control.

This invention relates to a resin-based nano-iron oxide-enhanced microbial preparation, a method for preparing the same, and its applications. In this invention, after mixing resin-based nano-iron oxide, the microbial preparation, and food waste, at room temperature, the resin-based nano-iron oxide enhances the decomposition of food waste by the microbial preparation through multiple actions, including promoting microbial growth, inducing high activity of functional proteases, and accelerating electron transport processes. The alternating aerobic and anaerobic environments allow the microbial preparation to exert its full effect, resulting in high biochemical activity of metabolites. A reaction under room temperature conditions of 3 to 5 hours can achieve a food waste reduction rate of 80% to 99%. This invention is technically simple, consumes little energy, does not cause secondary pollution, and effectively achieves the goals of food waste reduction, resource recovery, and detoxification.

The invention relates to the technical field of microbial preparation optimization and intelligent algorithm crossing, and particularly discloses a foraging algorithm for coupling dominant functional bacteria of targeted biological bacteria and host original flora, and the foraging algorithm simulates a coupled symbiotic system of the targeted biological bacteria and the host original flora into a flora society. Each to-be-optimized single-function bacterial strain is regarded as a'bacterial 'individual in the algorithm, the comprehensive effect of a flora on a specific target is used as a fitness function, global optimization is carried out through an improved chaos optimization bacterial foraging algorithm, and through the steps of flora proportion initialization, chemotaxis operation, breeding operation, migration operation and the like, the optimal bacterial foraging algorithm is obtained. The method provided by the invention solves the problems of complex work, long period and difficulty in obtaining a globally optimal solution in the traditional'trial-and-error method 'for preparing the composite flora, and provides core algorithm support for quickly, accurately and intelligently developing an efficient composite microbial preparation.

The invention discloses a preparation method of an ingredient containing waste milk and the ingredient. The ingredient is prepared from the following raw materials in parts by weight: 900-1100 parts of waste milk, 8-12 parts of a compound microbial preparation and 25-45 parts of a culture medium auxiliary material, the compound microbial preparation is prepared from bacillus subtilis, bacillus licheniformis, lysozyme and protease, wherein the mass ratio of the bacillus subtilis is 32 to 38 percent, the mass ratio of the bacillus licheniformis is 32 to 38 percent, the mass ratio of the lysozyme is 12 to 18 percent, and the mass ratio of the protease is 12 to 18 percent; the culture medium auxiliary material consists of protein powder, brown sugar powder and amino acid, and the mass ratio of the protein powder to the brown sugar powder to the amino acid is 1: 20: 5. According to the invention, the waste milk is used as a main raw material, so that the resource utilization of wastes is realized, the environmental pollution is reduced, and the production cost of the feed is reduced.

This invention discloses a method for co-culturing giant freshwater prawns (Macrobrachium rosenbergii) with shrimp, grass, and fish based on probiotic regulation, and its application. This invention constructs a three-dimensional ecological system with shrimp as the main body, grass as the protector, and fish as a supplement. Elodea and water spinach purify the water, silver carp and crucian carp decompose uneaten feed and feces, and a compound microbial preparation of Lactococcus gasseri C6a2 regulates water quality, while a biological bottom conditioner improves the bottom sediment, achieving precise ecological regulation of the aquaculture environment. This invention does not rely on chemical agents, effectively inhibits ammonia nitrogen accumulation, reduces disease incidence, and significantly improves the survival rate and size of giant freshwater prawns, achieving a win-win situation for both ecological and economic benefits.

The invention provides a plant lactobacillus PCFS-60493 and application of the plant lactobacillus PCFS-60493 in fermentation of low-sugar and low-acid beverages. The plant lactobacillus PCFS-60493 is preserved in China General Microbiological Culture Collection Center on November 25, 2025, and the preservation number is CGMCC No.36673; the strain can be used for preparing the low-sugar low-acid beverage, and the preparation method comprises the following steps: adding a proper amount of white sugar into fruit juice to enable the sugar degree to reach 15-25%, then adding a microbial preparation containing the plant lactobacillus PCFS-60493 into the fruit juice, and carrying out sealed standing fermentation at 37 + / -2 DEG C for 40-50 hours to obtain the low-sugar low-acid beverage. The strain can significantly reduce the content of total sugar and total acid in the beverage, degrade bitter components, and promote protein hydrolysis to generate amino acids to improve delicate flavor; the preparation process is simple, short in fermentation period and safe, realizes resource utilization of grape byproducts, is suitable for large-scale production of the high-polyphenol low-sugar fermented beverage, and has both economic and social benefits.

The invention relates to the technical field of biological fermentation process control, and discloses an agricultural microbial preparation fermentation deviation analysis and optimization system and method, and the system comprises a data collection and knowledge extraction module which is used for collecting historical fermentation data: data structuring, knowledge extraction and mining, and knowledge fusion and association; the system comprises an establishment module used for establishing a fermentation field knowledge graph containing entities and relationships between the entities, a normal fermentation trajectory graph generation module used for determining a successful fermentation case from historical fermentation data according to a final product quality index and generating a normal fermentation trajectory graph representing an ideal fermentation process, and a real-time data acquisition module used for acquiring real-time data of the normal fermentation trajectory graph. The acquisition module is used for acquiring real-time fermentation parameters of the current fermentation batch. According to the hybrid reasoning engine, firstly, candidate adjusting instructions are rapidly generated from historical successful deviation correction cases through case reasoning, then rule reasoning is utilized, and it is ensured that the finally generated scheme has the practical experience basis.

The invention provides a plant-growing bag suitable for an extreme environment and a planting method using the plant-growing bag. Firstly, the vegetation bag suitable for the extreme environment is disclosed and comprises a bag body, and the bag body is of a multi-layer bag film structure with the closed bottom and comprises an outer layer which is a supporting layer composed of high-strength moisturizing dense grain composite fibers; the middle layer is a functional layer attached to the inner side of the outer layer and is composed of a slow-release vegetative mass and a curing agent and / or a microbial preparation and / or a plant growth regulator; the inner layer is a micro-permeable layer arranged on the inner side of the middle layer and is composed of a high-strength degradable plant fiber micro-permeable film. The invention further discloses a planting method using the vegetation bag. The vegetation bag is good in water retention effect, remarkable in water and fertilizer saving effect, high in construction efficiency and suitable for afforestation in the extreme environment, and a sustainable scheme is provided for ecological safety of the extreme environment.

The application provides a growth promoting microbial preparation for relieving saline-alkali stress and application thereof, and belongs to the technical field of agricultural microorganisms. The microbial preparation provided by the application can significantly relieve the adverse effect of saline-alkali stress on the growth and development of corn, and can stably improve the growth indexes of corn such as plant height, stem diameter, fresh weight, dry weight, leaf area index and chlorophyll content in different saline-alkali degree plots, and continuously promotes the growth in the whole growth period of corn, especially promotes the grain fresh weight in the late growth period, which is beneficial to yield formation. Meanwhile, the different strains in the compound microbial preparation have good interaction ability, can play the synergistic effect of multiple bacteria and functional complementary effect, and further improve the adaptability and growth promoting effect of the microbial preparation in the complex soil environment. The application has the advantages of environmental friendliness, low cost, difficulty in producing drug resistance and the like, and has a good application prospect in the fields of agricultural yield increase in saline-alkali land and development of microbial fertilizers.

This invention discloses a natural polysaccharide-based multilayer composite encapsulated probiotic and its preparation method, relating to the field of functional food and microbial preparation technology. This invention provides metabolic substrates for the probiotics through a probiotic-prebiotic co-encapsulation core located in the innermost layer, enabling them to maintain physiological activity during encapsulation. The first anionic polysaccharide encapsulation layer uses sodium alginate, which forms a three-dimensional gel network in a calcium-ion-containing curing solution. In the acidic environment of the stomach, the carboxyl groups in the calcium alginate gel undergo carboxylation, and the network shrinks and densifies, effectively blocking the invasion of hydrogen ions and pepsin, thus physically isolating the probiotics from gastric juice. When the encapsulated probiotics enter the small intestine and colon, the chitosan in the second pH-responsive composite encapsulation layer undergoes a conformational change due to amino deprotonation. Simultaneously, phosphate and citrate ions in the outer environment exchange ions with calcium alginate, achieving a triggered release of the probiotics in the colon.

The disclosure relates to genetically modified microorganisms of the genus Bacillus, for the improvement of phenotypes of plants, for example nitrogen availability for non-leguminous plants. Included are novel strains of the microorganisms, microbial consortia, and agricultural compositions comprising the same. Furthermore, the disclosure teaches methods of utilizing the described microorganisms, microbial consortia, and agricultural compositions comprising the same, in methods for imparting beneficial properties to target plant species. In particular aspects, the disclosure provides methods of increasing desirable plant traits in agronomically important species, for example nitrogen fixation, utilization, regulation, uptake, acquisition, tolerance, and / or processing in plants.

The invention belongs to the technical field of microorganisms, and discloses clostridium butyricum ML5358 and application thereof.The clostridium butyricum ML5358 provided by the invention has the preservation number of CGMCC No.37000. The strain can effectively relieve mouse colitis induced by DSS and remarkably reduce expression of colon tissue inflammatory factors Il-6 and Il-1beta, and the preservation number of the clostridium butyricum ML5358 is CGMCC No.37000. The clostridium butyricum ML5358 is CGMCC No.37000. The clostridium butyricum ML5358 is CGMCC No.37000. In addition, skeletal muscle lipid metabolism can be regulated and controlled through the intestine-muscle axis, expression of fat synthesis and uptake related genes Srebf1, Acc1 and Fabp4 is specifically up-regulated, the content of intramuscular triglyceride is remarkably increased, and intramuscular fat deposition is promoted. According to the clostridium butyricum ML5358 and the application thereof, the functional limitation of traditional clostridium butyricum is broken through, and a new strain resource is provided for developing a novel microbial preparation or a feed additive capable of improving intestinal health and improving livestock and poultry meat quality.

The invention relates to the technical field of microbial preparations for agricultural environment restoration, and provides a microbial agent for reducing pesticide residues in soil, which is characterized by comprising the following components in parts by weight: 30-60 parts of ochrobactrum having viable count of more than or equal to 5 * 10 < 8 > cfu / g; the spore number is greater than or equal to 2 * 10 < 8 > cfu / g. The final residues of imidacloprid and difenoconazole in the compounded microbial agent are reduced to about 0.20 mg / kg, balanced and efficient removal is realized, and the possible unbalance situation of'degrading one pesticide and remaining one pesticide 'in the traditional method is avoided.

The invention belongs to the field of microbial preparations, and particularly relates to a protection method of a microzyme freeze-dried fungicide based on low-liquefied starch. On one hand, the invention provides a freeze-drying protective agent. The freeze-drying protective agent is obtained by carrying out enzymolysis on a starch solution with amylase for 20-200 minutes, centrifuging and freeze-drying. On one hand, the invention provides a protection method of a saccharomycetes freeze-drying bacterial agent, which comprises the following steps: adding the freeze-drying protective agent into saccharomycetes resuspended thalli, standing, removing supernatant to obtain paste, and freeze-drying the paste. By using the freeze-dried microbial inoculum provided by the invention, the survival rate of the saccharomycetes freeze-dried microbial inoculum is remarkably improved.

The application provides a preparation method of an antioxidant Aeschynomene indica L. probiotic fermentation liquor, and belongs to the technical field of biological fermentation and microbial agents. The method comprises the following steps: step one, respectively preparing an Aeschynomene indica L. extract and a probiotic seed liquor; and step two, inoculating the seed liquor into the Aeschynomene indica L. extract or a culture medium containing the Aeschynomene indica L. extract for fermentation to obtain the Aeschynomene indica L. probiotic fermentation liquor. The Aeschynomene indica L. probiotic fermentation liquor provided by the application can effectively maintain the viable count of probiotics so that the probiotics can exert the probiotic effect, and the Aeschynomene indica L. water extract itself also has multiple probiotic activities, and the content of antioxidant metabolites is obviously increased after fermentation.