The invention provides a feed for breeding crawfish in a rice field and a preparation method thereof. Raw materials of the feed comprises: corn flour, wheat, rapeseed meal, vital gluten, soya bean lecithin, konjaku flour, sodium alginate, chitosan, xanthan gum, fish oil, lysine, vitamin C, disodium succinate, citric acid, bacillus and nitrobacter. The preparation method of the feed comprises: firstly, adding the corn flour, the wheat, the rapeseed meal, the vital gluten and the konjaku flour into water; after carrying out heating and heat preservation, adding the sodium alginate and the xanthan gum, and carrying out heat preservation and stirring; after cooling, adding the soya bean lecithin, the chitosan, the fish oil, the vitamin C, the citric acid and the disodium succinate, and carrying out heating and heat preservation; after cooling, adding the bacillus and the nitrobacter, stirring and carrying out extrusion granulation to obtain the feed. According to the feed disclosed by the invention, not only is the immunity of the crawfish effectively reinforced, but also water body pollution caused by the feed can be effectively reduced, the survival rate of the crawfish is improved, and occurrence of diseases and insect pests is reduced.