12 results about "Nitrobacter" patented technology

The invention discloses a microbial organic fertilizer which comprises the following raw materials in parts by weight: 35-45 parts of a microbial agent, 20-25 parts of an organic fertilizer, 15-20 parts of amino acid, 10-15 parts of sodium humate and 10-15 parts of filler particles, the microbial agent is prepared from bacillus subtilis, lactobacillus and nitrobacter according to the weight ratio of 3: 1: 1; the organic body fertilizer is obtained by mixing 30-45 parts of rice straw, 20-25 parts of livestock manure and 10-14 parts of water, stacking the mixture at 45 DEG C for 30 days, and finally crushing the mixture in a crusher to pass through a 100-mesh sieve. According to the microbial organic fertilizer, the microbial agent is matched with the organic fertilizer, the amino acid and the sodium humate are added, meanwhile, the filler particles are added, and the filler particles are matched into the system, so that the fertilizer quality of the microbial organic fertilizer is enhanced, and the heavy metal removal efficiency of the product is improved.

The invention relates to the technical field of water pollution control and treatment, in particular to a nitrifying bacteria consortium, a preparation method thereof and application of the nitrifying bacteria consortium in wastewater treatment. The nitrobacteria consortia comprises nitrosomonas sp. And nitrobacter winogradsky, and a compact and spherical cenobium structure is formed; the nitrification rate of the continuous passage of the nitrifying bacterium consortium is stabilized between 200-300mg NH4 < + >-N / L / h, so that the long-term stability of the nitrifying bacterium community is ensured; meanwhile, ammonia nitrogen can still be efficiently degraded under the extreme environmental conditions of high salt, high alkalinity, high aniline content and the like.

The invention discloses nitrous acid and ammonia nitrogen resistant nitrobacter (Nitrobacter sp.) TKz-YC01, the preservation number of the nitrous acid and ammonia nitrogen resistant nitrobacter (Nitrobacter sp.) TKz-YC01 is GDMCC No: 65729, the nitrous acid and ammonia nitrogen resistant nitrobacter (Nitrobacter sp.) TKz-YC01 has been preserved in Guangdong Microbial Culture Collection Center on March 7, 2025, is called GDMCC for short, has the preservation address of Nitrobacter sp., and is classified and named as Nitrobacter sp., and the preservation name of the nitrous acid and ammonia nitrogen resistant nitrobacter (Nitrobacter sp. TKz-YC01 is Nitrobacter sp. The nitrobacter can effectively degrade nitrite, cyanate and / or ammonium salt in water, and the nitrobacter can still effectively remove nitrogen under the impact of 30 mM ammonium salt (ammonium chloride) and 50 mM nitrite (sodium nitrite).

The present disclosure belongs to the technical field of bioengineering. Specifically, the present disclosure relates to a Nitrobacter strain and the use thereof for water purification. Nitrobacter strain SZG-NOB-003 of the present disclosure requires no additional carbon source, has a relatively high affinity for nitrite, exbihits multiple environmental and antibiotic resistance, and can stably exert a purification effect and improve water quality in harsh or complex environments.

The invention relates to a method for composting black soldier fly feces by using duckweed and charcoal, which comprises the following steps: collecting black soldier fly feces and biological wastes, weighing the black soldier fly feces and biological wastes, and spraying a microbial complex microbial inoculant which is bacillus subtilis and bacillus stearothermophilus. A combination of nitrosomonas, nitrobacter, white-rot fungi and streptomyces; obtaining a primary compost; laying biochar on the surface of the preliminary compost, and then laying duckweed on the outer surface of the biochar layer; stacking in sunlight at normal temperature; and after the temperature of the compost body is reduced to 50 DEG C or below, turning the outer charcoal and the duckweed into the compost, and covering the surface of the compost body with the charcoal after turning to complete the composting. The adopted duckweed can utilize nitrogen discharged by compost, and the biochar can adsorb the nitrogen discharged by the compost, so that the duckweed can better utilize the nitrogen discharged by the compost, the nitrogen in the compost is immobilized and converted, the nitrogen loss of the compost is reduced, and the foul smell of a compost body is reduced.

The application discloses a method for improving the nitrification efficiency under alkaline conditions by regulating extracellular polymeric substances and ion transport. The method induces the secretion of more acidic polysaccharides and acidic organic substances in the extracellular polymeric substances of nitrifying bacteria and regulates the activities of anti-transport proteins and F0-F1 ATPase by gradually increasing the pH value of the system, the operation days and other comprehensive strategies, thereby promoting the intracellular H + input to maintain the intracellular pH stability. At the same time, the biological mineralization effect of carbonic anhydrase is utilized to accelerate the consumption of extracellular OH ‑ , improve the alkaline environmental adaptability and nitrification performance of the nitrification sludge, and significantly enrich Nitrosomonas and Nitrospira and other alkali-tolerant nitrifying bacteria, and the average ammonia nitrogen removal rate of the system under the condition of pH 10.0 can be as high as 96.6%.

The present invention relates to the technical field of sewage treatment, and specifically discloses a sewage treatment method that is beneficial to reducing the amount of sludge production, and the sewage treatment method that is beneficial to reducing the amount of sludge production includes the following steps: step 1), adding a flocculant to sewage, filtering, and obtaining pretreated water; step 2), adding a composite bacterial liquid to the pretreated water, stirring for 3-4 days; step 3), sterilizing, filtering, and obtaining clean water; the culturing method of the composite bacterial liquid is as follows: step 01), taking sewage, concentrating, and obtaining concentrated sewage; step 02), inoculating Nitrospira, Nitrobacter vervetii, and Kingella denitrificans in the concentrated sewage, culturing to an OD value of ≥1, and obtaining a composite bacterial liquid. The present invention has the advantage of reducing sludge production during sewage treatment.

The invention relates to a halophilic denitrifying bacterium LH-B.0012 as well as a microbial inoculum and application of the halophilic denitrifying bacterium LH-B.0012. The bacterial strain is classified and named as Salimicrobium salinigenes, and the preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) NO: 16317. The halophilic denitrifying bacterium LH-B.0012 disclosed by the invention belongs to facultative anaerobic bacteria, and has wide salt tolerance; the high salt-resistant denitrification total nitrogen removal capacity is achieved, nitrate nitrogen and nitrite nitrogen can be removed in a limit denitrification mode within the salinity range of 0.5%-25%, and the removal rate can reach 99.5% or above; the low-temperature-resistant performance is achieved, and the denitrification efficiency can be remarkably improved under the low-temperature condition of 6-12 DEG C. The invention further discloses an autotrophic and heterotrophic synergistic efficient total nitrogen removal bacterial agent containing the LH-B.0012 strain. The total nitrogen removal efficiency of the microbial inoculum is 20% or above higher than that of independent heterotrophic or independent autotrophic denitrification nitrogen removal efficiency, and the denitrification nitrogen removal efficiency of the system can be improved by 20% or above under the low-temperature condition of the microbial inoculum.

The invention relates to a tetracycline-resistant pseudomonas aeruginosa denitrifying bacterium, and belongs to the technical field of sewage treatment, the tetracycline-resistant pseudomonas aeruginosa denitrifying bacterium specifically comprises an anaerobic denitrification drug-resistant strain, the strain of the strain is pseudomonas aeruginosa, the name of the anaerobic denitrification drug-resistant strain is Pseudomonas aeruginosa YF2024, and the preservation number of the anaerobic denitrification drug-resistant strain is CGMCC NO.34912; the anaerobic denitrification drug-resistant strain is in the form of a short rod, the length is 1.0-1.5 mu m, the diameter is 0.6-0.7 mu m, gram staining is negative, VP reaction is positive, and drug sensitivity to tetracycline is negative. The denitrifying bacterium provided by the invention can still keep the nitrate nitrogen degradation rate of 98% under the conditions of high tetracycline and high nitrogen source.

The invention relates to an MBBR (Moving Bed Biofilm Reactor) unit (1) that reduces the amount of total nitrogen (TAN, NH3N), which yields fatal consequences for living organisms in living systems; requires no downtime for system maintenance; and maximizes the efficiency of the nitrification process by activating Nitrobacter and Nitrosomonas type bacteria. More specifically, the present invention relates to an MBBR unit (1) that allows for homogeneous water distribution and high-performance bio-media movement, which improves the performance values of the nitrification process vital for living systems, provides longevity, and increases efficiency while reducing water consumption.

The invention discloses a method for improving nitration efficiency under alkaline conditions by regulating extracellular polymeric substances and ion transport. According to the method, through comprehensive strategies of gradually increasing the pH value of the system, controlling the number of operation days and the like, nitrobacterium extracellular polymeric substances are induced to secrete more acidic polysaccharides and acidic organic matters, and antitransporter and F0-F1 ATP enzyme activity are regulated and controlled, so that the input of intracellular H < + > is promoted to maintain the intracellular pH stable. Meanwhile, the consumption of extracellular OH <-> is accelerated by utilizing a biomineralization effect participated by carbonic anhydrase, the alkaline environment adaptability and nitrification performance of the nitrification sludge are improved, alkali-resistant nitrifying bacteria such as Nitrosomonas and Nitrospira are remarkably enriched, and the average ammonia nitrogen removal rate of the system can stably reach 96.6% under the condition that the pH is 10.0.

The invention discloses a water pollution treatment method in an energy transfer mode. The water pollution treatment method mainly comprises the following steps that oysters are bred in a pond in a hanging mode, and mullets, sea cucumbers and other filter-feeding and sponging marine organisms are bred in the pond; marine plants such as undaria pinnatifida, gelidium amansii and ulva pertusa which are naturally generated in a pond are utilized, and a small part of beneficial microbial populations such as nitrobacter and bacillus are added; putting a part of unicellular algae to decompose and absorb soluble pollutants such as nitrogen and phosphorus dissolved in the water body; on the basis of the energy transfer principle, a multi-nutrient-level full-ecological-system aquaculture tail water purification system is constructed, an energy transfer channel is opened, and aquatic organisms at different nutrient ecological nichs are utilized to comprehensively treat insoluble granular nutrients and soluble inorganic nitrogen and phosphorus pollutants in a polluted water body in a three-dimensional manner. The organisms in each level of the whole ecological system in the water body are used as energy carriers to respectively absorb nutrition, convert energy and actively transfer energy to the highest level to become aquatic organism energy, the water body is separated out, the water quality is improved, and pollution is treated.