62 results about "Nitrobacter" patented technology

A wastewater treatment method employing chemicals for disinfection, and precipitation of suspended solids, heavy metals and phosphates before subsequent aerobic bacterial biological treatment using Nitrosomonas and Nitrobacter bacteria to remove ammonia and nitrates / nitrites and BOD compounds to produce recovered treated wastewater suitable for open water discharge.

The invention provides a compound microorganism agent for treating a black and odorous water body. The compound microorganism agent comprises compound bacteria and a compound enzyme preparation, wherein the compound enzyme preparation comprises candida tropicalis, candida utilis, bacillus natto, bacillus subtilis, bacillus licheniformis, nitrobacter, achromobacter denitrificans, nitrosomonus, acinetobacter, lactobacillus, aspergillus niger, aspergillus oryzae and photosynthetic bacteria; the compound enzyme preparation comprises protease, amylase, lipase, sucrase, and cellulase.

The invention discloses an immobilized microbial preparation which is prepared through the following steps: S1, fermentation culturing strain: respectively inoculating bacillus subtilis, bacillus megatherium, nitrobacter and nitrosomonas in an enrichment medium to be cultured; S2, mixing thallus to obtain mixed thallus liquor; S3, immobilizing PVA-sodium alginate, namely uniformly mixing PVA gel with the mixed thallus liquor, dripping the mixture into a boric acid solution containing CaCl2 while continuously stirring at a low speed, immobilizing and crosslinking the mixture, taking out the mixture, washing the mixture through deionized water, and freeze drying the mixture to obtain the immobilized microbial preparation. The invention further discloses a method of treating meat product processing waste water by utilizing the immobilized microbial preparation. The immobilized microbial preparation disclosed by the invention has the beneficial effects that the microorganism categories are various, various pollutants in the waste water can be degraded, the degrading speed is fast and the capability is strong; the immobilized microbial preparation is suitable for changes of water quality and pH; the sludge yield is small, the products are liable to separate, the stability is good, the technique operation is simple, and the production cost is low.

One kind method and equipment for life sewage's denitrogenation and dephosphorization belongs to the water treatment domain. The method is that the sewage through obligate baffle reactor obligate sludge layer, spreads the phosphorus on the iron filings padding layer, remove the organic pollutant microorganism in obligate way, the not transformed organic pollutant is degraded in aerobic sunk-style biofilter biomembrane, the ammonia nitrogen transforms to nitrate nitrogen, then is removed again in obligate region by biomembrane counter- nitrobacter, again passes through the precipitation then discharges. The installment is made up of obligate baffle reactor mutually linking and in turn, the aerobic sunk-style biofilter and settling basin. The obligate baffle reactor compose of 3 - 4 baffle in, the partition board, obligate sludge separate room , 1st traverse has the iron filings padding layer, has counter- tube, in the aerobic sunk-style biofilter has the padding layer.

The invention aims to provide a microbial flora that can perform degradation of ammonia nitrogen at low temperature. The microbial flora includes Nitrosomonas, Pseudomonas, Bacillus and Nitrobacter. The microbial flora is separated and screened from active sludge and can perform degradation of ammonia nitrogen in sewage at low temperature; when bacterial strain quantity reaches 10<9>-10<11> cfu / ml and initial ammonia nitrogen concentration is 5.0 mg / l, sodium acetate, as a carbon source, is added according to that C / N ratio is 2; under the conditions that temperature is 2 DEG C, pH value is 7.2 and rotation speed is 150 rpm (dissolved oxygen is higher than 2 mg / L), the ammonia nitrogen is degraded for 30 min, wherein degradation rate can reach 9.350 mg(NH4<+>) / L*h. Compared with conventional single bacterial strain, the compound microbial flora can greatly increase the degradation rate of the ammonia nitrogen in sewage. The invention provides a high-quality and high-effective bacterial strain for sewage treatment.

The invention provides a composite microbial agent for treating contaminated water and a preparation method thereof. The composite microbial agent for treating contaminated water comprises photosynthetic bacteria, bacillus, nitrosobacter, nitrobacter, denitrifying bacillus and biological enzymes. The photosynthetic bacteria, bacillus, nitrosobacter, nitrobacter and denitrifying bacillus are obtained by culture and domestication of microbes extracted from natural water, and the combined bacteria have characteristics of species diversity, high activity, high efficiency and good stress resistance. The composite microbial agent can effectively stimulate the activity of microorganisms in water, accelerate the growth and reproduction of microorganisms and provide a good support for in-situ remediation in treatment on waste water in rivers, lakes and rivers.

The invention discloses a preparation method of a compound microbial inoculum for degrading domestic sewage, relating to a preparation method of compound microbial inocula and solving the problem in the prior art of low nitrogen and phosphorus removal rate of sewage in artificial wetland at a low temperature environment. The preparation method comprises the following steps of: I, respectively inoculating pseudomonas, bacillus, nitrobacter, nitrosomonas and bacillus megatherium in a sterilized domestic sewage culture medium for culturing to obtain a respective inoculum; II, preparing COD (Chemical Oxygen Demand) degradable compound bacteria, preparing nitrobacter and nitrosomonas compound inocula, and taking the bacillus megatherium inoculum as phosphorous removal bacteria; and III, mixing the COD degradable compound bacteria, the nitrobacter and nitrosomonas compound inocula and the phosphorous removal bacteria under the condition of the same bacterial count. The prepared compound microbial inoculum for degrading domestic sewage is strong in adaptability to the environment, suitable for microorganism enhanced sewage treatment in the northern area, the ammonia nitrogen conversion rate of sewage can reach 95%, the total nitrogen conversion rate reaches 82%, the COD conversion rate reaches 60%, and the total phosphorus conversion rate is about 75%.

The invention provides a zeolite-calamus constructed wetland sewage disposal system as well as a method utilizing the constructed wetland system to treat domestic sewage. The zeolite-calamus constructed wetland sewage disposal system mainly comprises a tank body, a filler filled in the tank body, aquatic plants planted on the surface of the tank body as well as a water distribution pipe and a water collecting pipe distributed in the tank, the filler is mainly clinoptilolite, Nitrobacter opacus Sack and Nitrosomonas monocella Nelson are put into the filler, and the aquatic plants planted on the surface of the tank body are calami. The system and method adopt clinoptilolite which has good ammonia nitrogen removal effects to serve as the filler of the constructed wetland, in order to improve the treatment efficiency of zeolite, special nitrifying bacteria under artificial culture are screened to regenerate the zeolite, and the constructed wetland system has advantages of high ammonia nitrogen removal capacity, low investment and running costs and simple operation.

The invention discloses nitrobacter winogradsky and nitrobacteria-denitrifying bacteria complex microbial inoculants, a production method and application. Nitrobacter winogradsky is deposited at the China Center for Type Culture Collection with the accession number of ((i) Nitrobacter Winogradsky i ( / i)) Y3-2, CCTCC NO: M2014203. The nitrobacter winogradsky has a function of effectively treating nitrite, and is mixed with nitrosomonas europaea and pseudomonas stutzeri to obtain the complex microbial inoculants, and the complex microbial inoculants can efficiently remove nitrogen in water, and can be used for treating aquaculture water, livestock breeding sewage or industrial sewage.

The invention discloses zeolite modified macromolecule suspension biological carrier based wastewater reinforcement and nitrification process, and belongs to the technical field of sewage treatment. Through the improvement on the core part, a suspension biological carrier, of moving bed biofilm process and biofilm and activated sludge composite process, the suspension biological carrier can have functions of adsorbing ammonia nitrogen and enriching nitrobacteria by adding a functional material; and the zeolite modified macromolecule suspension biological carrier is put into a MBBR / IFFAS reactor according to a certain proportion, so that the enrichment effects of nitrosomonas & nitrobacter on the carrier can be improved, the abundance of functional flora in a reaction system can be enhanced, and therefore, the nitrification effects of the system can be reinforced, and effluent ammonia nitrogen can meet requirements for discharging.

The invention discloses a strain formula for treating a black and odorous water body. The strain formula is characterized by comprising strains as follows: photosynthetic bacteria, bacillus, nitrosifying bacillus, nitrobacter, denitrifying bacillus, bio-enzyme, clostridium papyrosolvens, actinomycetes, paracoccus denitrificans and compound microorganisms, wherein the compound microorganisms comprise pseudomonas, vibrio, flavobacterium, Nitrosococcus, thiobacillus thiooxidant, Bacillus subtilis, bacillus licheniformis, Aneurinibacillus, saccharomycetes, denitrifying coccus and actinomycetes. The invention further discloses a preparation method of the strain formula for treating the black and odorous water body. When the strain formula is applied to treatment of wastewater of rivers and lakes, the microbial agent formula has effects that activity of microorganisms in the water body can be effectively stimulated, growth and reproduction of the microorganisms are accelerated, and good support function is provided for in-situ remediation.

The invention discloses a bacterial strain and complex microbial agent for bioremediation of petroleum polluted water, and a preparation method and application of the agent. The invention particularlydiscloses Pseudomonas putida which is used for bioremediation of petroleum and petroleum product pollution. On one hand, the invention discloses a complex microbial agent A. The complex microbial agent takes the Pseudomonas putida as an active core, is supplemented with bacillus pumilus, bacillus subtilis and bacillus licheniformis, greatly increases the petroleum hydrocarbon degradation rate through a synergistic effect, and has the application potential of efficiently repairing petroleum and petroleum product pollution. On the other hand, the invention discloses a complex microbial agent Bwhich comprises the Pseudomonas putida, and Nitrosomonas europaea and Nitrobacter winogradsky with a nitrification function, synergistically degrades petroleum and petroleum products, and increases the ammonium nitrogen removal rate at the same time.

The invention provides nitrobacter winogradsky XY-01 with the preservation number of CGMCC (China General Microbiological Culture Collection Center ) 10171, the nitrobacter winogradsky XY-01 can be subjected to fermentation cultivation by adopting a batch feeding way, a cultivated product can be used for removing nitrite nitrogen in aquatic water, the problem of nitrite accumulation in the aquatic water can be effectively solved, the fish and shrimp yield is increased, and the nitrobacter winogradsky XY-01 has a wide application prospect in the field of aquaculture.

The invention discloses a compound microorganism water-purification agent and a preparation method thereof. The water-purification agent is composed of bacillus megaterium, bacillus circulans, rhodopseudomonas palustris, nitrobacter and lactobacillus plantarum. In a culture process, after all strains are cultured separately, mixed culture of the strains is carried out according to the ratio of all strains, so that the finished product is obtained finally. The density of each of five strains in the agent can reach 7-10*10<8> / ml / . By adopting the method provided by the invention, all strains can grow effectively, so that effective microorganism concentration of the compound microorganism water-purification agent is ensured; after the compound microorganism water-purification agent is fed into recycled water receiving water, the effective concentration of microorganisms is high, various microorganism strains play corresponding roles to effectively remove COD, ammonia nitrogen, TN, TP and other pollutants in water originally, particularly, the removal rate for TN is high, and the compound microorganism water-purification agent can change chromaticity and odor of water, and has an obvious water purification effect.

The invention discloses a microbial water purifying agent for loach culture water and a preparation method of the microbial water purifying agent. The preparation method comprises the following steps of adding 30-50 parts by weight of magnetic silicon balls with surfaces modified with C8 and C18 alkyl chains to 300-500 parts by weight of water; adding 100-150 parts by weight of microbial reagents which include the following components in parts by weight: 60-80 parts of rhodopseudomonas palustris, 60-80 parts of synechococcus bacteria, 10-30 parts of nitrococcus, 20-40 parts of nitrobacter Winogradsky, 20-30 parts of pseudomonas aeruginosa, 10-20 parts of paracoccus denitrificans, 3-5 parts of beggiatoa, 3-5 parts of thiobacilli, 30-60 parts of infusorian, 30-60 parts of plantlike flagellate, 30-50 parts of dunaliella tertiolecta and 30-50 parts of chlorella vulgaris; then adding to 60-100 parts by weight of beef extract peptone agar mediums for culture. The microbial water purifying agent disclosed by the invention can achieve the purpose of purifying the water inside a culture pond by being added to a loach culture pond.

The invention provides a feed for breeding crawfish in a rice field and a preparation method thereof. Raw materials of the feed comprises: corn flour, wheat, rapeseed meal, vital gluten, soya bean lecithin, konjaku flour, sodium alginate, chitosan, xanthan gum, fish oil, lysine, vitamin C, disodium succinate, citric acid, bacillus and nitrobacter. The preparation method of the feed comprises: firstly, adding the corn flour, the wheat, the rapeseed meal, the vital gluten and the konjaku flour into water; after carrying out heating and heat preservation, adding the sodium alginate and the xanthan gum, and carrying out heat preservation and stirring; after cooling, adding the soya bean lecithin, the chitosan, the fish oil, the vitamin C, the citric acid and the disodium succinate, and carrying out heating and heat preservation; after cooling, adding the bacillus and the nitrobacter, stirring and carrying out extrusion granulation to obtain the feed. According to the feed disclosed by the invention, not only is the immunity of the crawfish effectively reinforced, but also water body pollution caused by the feed can be effectively reduced, the survival rate of the crawfish is improved, and occurrence of diseases and insect pests is reduced.

The present invention refers to a process to increase the concentration of micro organisms' colonies which are formed on the surface of Gramineas bambusoideae in a batch and / or continuous flow process which utilizes biomass as a filtration means in order to remove nitrates as well as organic and inorganic impurities from water and / or from affluents in which a step of adsorption is followed by a step of biological degradation by the anaerobic digestion of micro organisms of the types Pseudonomas SP (Nitrosomonas, Nitrosococus, Nitrobacter, Azobacter, Azotomas and Rhixobium). In accordance to the present invention, the addition of about 200-300 ppm of sodium acetate to the solution fed to the reactor, maintaining a relation of 2:1, C:N, provides an efficiency increase of 80% to 98% regarding the removal of nitrate as well as organic and inorganic soluble matter from the water.

The invention discloses an aquaculture pond sediment improver which comprises raw materials in parts by weight as follows: 20-30 parts of construction waste regenerated particles, 5-10 parts of medical stone, 1-5 parts of chitin and 10-20 parts of a probiotics mixed preparation, wherein a nitrobacterium reagent adopts a nitrosomonas, nitrobacter and actinomycetes mixture. According to the improver, the regenerated waste particles having specific particle size after ball milling have excellent adsorption, not only is the problem of construction waste disposal solved effectively, but also a novel improver adsorption subject with better adsorption capacity is provided; the probiotics mixed preparation can effectively promote decomposition of pond bottom sediment, so that the water quality is optimized; the improver after effective compounding of the construction waste regenerated particles, the medical stone, the chitin and the probiotics mixed preparation can be normally used in an aquaculture process, aquaculture animals are not needed to be transferred to another pond, and time is saved; the content of ammoniacal nitrogen, nitrite, hydrogen sulfide and the like in the pond is reduced remarkably after the improver is used for 1-3 days.

The invention discloses a microbial water purifying agent used for grass shrimp culture wastewater and a preparation method thereof. The preparation method: add 30-50 parts by weight of magnetic silicon spheres with surface-modified C8 and C18 alkyl chains to 300-500 parts by weight of water; add 150-200 parts by weight of microbial reagents: 40-70 parts of Rhodomicrobacteria, 40-50 parts of Rhodobacter sphaericus, 20-40 parts of Nitrosomonas, 20-30 parts of Nitrobacter victoria, 10-30 parts of Pseudomonas stutzeri, 20-40 parts of Phytophthora, Thiobacillus 4- 5 parts, 2-5 parts of Thiobacillus beinerii, 50-80 parts of nematodes, 30-40 parts of vibrating worms, 20-40 parts of Dunaliella, 20-40 parts of chlorella; then add 70-90 parts by weight of beef extract Cultured on peptone agar medium. Putting the microbial water purifying agent of the present invention into the grass shrimp culture pond can achieve the purpose of purifying the water in the culture pond.

The invention discloses a microbial preparation for aquiculture. The microbial preparation comprises the following components in parts by weight: 3-5 parts of Bacillus subtilis, 3-5 parts of Bacillus licheniformis, 3-4 parts of Bacillus cereus, 3-4 parts of Bacillus megaterium, 2-3 parts of Bacillus polymyxa, 2-3 parts of Lactobacilli, 2-3 parts of Streptococcus lactis, 0-1 part of pseudomonas, 0-1 part of Nitrosomonas, 0-1 part of Nitrobacter and 0-1 part of thiobacillus. The microbial preparation for aquiculture is capable of thoroughly implementing disinfection, cutting off the virus infection, purifying the water, enabling the purified water to achieve the harmless standard, enhancing the aquiculture survival percent and the like, is natural and nontoxic, and thus, has wide market prospects in popularization of microbial preparations for aquiculture.

The invention relates to an ammonia nitrogen removal agent and a method for treating sewage by utilizing the agent, relates to the field of sewage treatment, and provides the ammonia nitrogen removalagent capable of improving a denitrifier propagation ability and with high shock resistance, and a method for treating the sewage by utilizing the agent. The ammonia nitrogen removal agent is preparedfrom the following components: 8 to 10 parts of arthrobacter globiformils, 15 to 20 parts of paracoccus denitrification, 10 to 12 parts of bacillus subtilis, 8 to 10 parts of nocardia amarae, 15 to 20 parts of nitrobacter winogradsky, 20 to 25 parts of pseudomonadaceae K50, 20 to 25 parts of pseudomonadaceae TR2, and 2 to 5 parts of snail stoste. The appropriate cultures are matched reasonably, so that a nitration-denitrification effect achieves the best; the snail slime is added into the agent, so that the cultures are promoted to grow and propagate, an ammonia nitrogen treatment effect is improved, and the shock resistance is improved; coconut oil fatty acid diethanol amide converts ammonium at an ion state into free ammonia, so that a denitrification progress of whole denitrification culture is accelerated; in addition, only N2 is produced during a denitrification process, and the production of N2O products in a sewage treatment system is reduced, so that the environment is protected.

A real-time fluorescent quantitative PCR detection method of nitrite oxidizing bacteria from a sewage treatment plant belongs to the technical field of biological sewage treatment. According to the invention, two genuses of nitrite oxidizing bacteria from a sewage treatment plant, namely nitrobacter and nitrospira, can be detected. All nitrite oxidizing bacteria can be analyzed, and dynamic changes of each genus can be analyzed. By a SYBR Green fluorochrome method with good versatility and low cost, real-time fluorescent quantitative standard curves of the two genuses of nitrite oxidizing bacteria are established. Correlation coefficients of the two standard curves are respectively 0.998 and 0.997, and amplification efficiency is between 100% and 110%. The established standard curves are utilized to monitor and identify nitrite oxidizing bacteria in a sewage treatment system. The invention provides an economical, simple and reliable real-time fluorescent quantitative PCR detection method of nitrite oxidizing bacteria from a sewage treatment plant.

The invention provides a composite strain biological fluidized bed membrane reactor, which comprises a plurality of reaction tanks, a biological membrane carrier and an aeration mechanism, and is characterized in that the biological membrane carrier is provided with composite strains, and the composite strains comprise composite oil removing bacteria, composite phosphorus accumulating bacteria, composite denitrifying bacteria and composite desulfurizing bacteria. Preferably, the composite oil removing bacteria comprise bacillus, saccharomycetes and micrococcus, the composite phosphorusaccumulating bacteria comprise aeromonas, pseudomonas and acinetobacter, the composite denitrifying bacteria comprise nitrosomonas and nitrobacter, and the composite desulfurizing bacteria comprise thiobacillus, pseudomonas and acinetobacter. By the adoption of the above scheme, petroleum, gasoline hydrocarbons, phosphorus-containing substances, ammonia nitrogen, nitrites and sulfides in water can be wellremoved, rapid establishment of nitrifying bacteria, phosphorusaccumulating bacteria and desulfurization bacteria in a sewage system is promoted, and the effluent quality is improved.

The invention relates to the field of biotechnology, in particular to a combined fermentation method of a nitrifying-denitrifying bacteria composite microecological preparation. The composite microecological preparation consists of Nitrobacter winogradsky KS05 and denitrifying bacteria KS03. The combined fermentation method specifically includes the following steps: preparing a strain; preparing culture media including a denitrifying bacteria culture medium and a Nitrobacter culture medium; inoculating the Nitrobacter culture medium with the Nitrobacter winogradsky KS05 while maintaining the concentration of sodium nitrite; and after culture of the nitrifying bacteria, adding the denitrifying bacteria culture medium on the basis, inoculating the denitrifying bacteria KS03, and when the content of nitrite and nitrate in the culture medium reaches 0 mg / l, ending the mixed culture. The staged continuous cultivation of nitrifying bacteria and denitrifying bacteria of the present inventioncan complete the nitrification and denitrification culture enrichment problems in a short time, save time and cost, improve fermentation efficiency, and reduce the cost in actual production.