331 results about "Nitrifying bacteria" patented technology

The invention discloses a composite denitrification accelerant and a preparation method thereof, and belongs to the technical field of sewage treatment. The carbon source composite material is prepared from the following raw materials in parts by weight: 30-50% of a carbon source, 2-5% of vitamins, 2-5% of amino acids, 1-3% of a biological stimulant, 1-2% of signal molecules, 1-5% of metal elements, 3-5% of a pH regulator and 25-60% of water. Through metabolites such as vitamins, amino acids and biological stimulants, growth and reproduction of denitrifying bacteria are promoted, and functions of related enzymes in the denitrification process are improved. The signal molecule optimizes the metabolic activity of bacteria by regulating and controlling the expression of genes, related to the denitrification process, of the denitrifying bacteria. The metal ions can enhance the activity of the denitrifying enzyme, so that the efficiency of denitrification treatment is improved. According to the denitrification accelerant, the denitrification process is promoted through the synergistic effect of three aspects of growth and reproduction of denitrifying bacteria, expression of denitrification genes and improvement of activity of denitrification-related enzymes.

The invention discloses a device and a method for realizing low-carbon-nitrogen-ratio sewage autotrophic and heterotrophic coupled deep nitrogen and phosphorus removal based on a continuous flow membrane aeration bio-membrane reactor. A hollow fiber membrane is arranged in the continuous flow reactor, and an oxygen concentration gradient is formed through a bubble-free membrane aeration technology. In the biomembrane area, spatial partitions of functional bacteria such as nitrifying bacteria and anaerobic ammonium oxidation bacteria are established, and the two anoxic areas realize efficient nitrogen removal through short-cut denitrification coupling anaerobic ammonium oxidation (PD / A) and synchronous nitrification and denitrification. In the aspect of phosphorus removal, the system adopts an anaerobic-aerobic operation mode, phosphorus-accumulating bacteria release phosphorus anaerobically and absorb excessive phosphorus aerobically, and finally, phosphorus removal is realized through residual sludge discharge. The system integrates an internal carbon source extraction process, recovers a sludge carbon source and supplements an electron donor required by denitrification, and reduces the operation cost and carbon emission. The continuous flow MABR denitrification system enhances the denitrification efficiency of the PD / A system, is suitable for large-scale operation, and efficiently and stably realizes deep denitrification of a low-carbon-nitrogen-ratio urban sewage treatment plant.

The present application relates to the field of municipal wastewater treatment, and particularly relates to a highway service area wastewater treatment device and method based on AOOA-MBBR process. The device comprises a first anoxic tank, a first aerobic tank, a second aerobic tank and a second anoxic tank connected in sequence, and is filled with MBBR filler respectively, and the volume of the filled MBBR filler is taken as the effective volume; the first aerobic tank and the second aerobic tank are respectively provided with an aeration device; the first anoxic tank is used for inoculating hydrolytic bacteria and denitrifying bacteria; the first aerobic tank is used for inoculating hydrolytic bacteria, nitrifying bacteria and denitrifying bacteria; the second aerobic tank is used for inoculating nitrifying bacteria, and the second anoxic tank is used for inoculating hydrolytic bacteria and denitrifying bacteria; the second aerobic tank is connected with the first anoxic tank through a reflux pipe, and the reflux pipe is provided with a reflux power assembly. The present application solves the problems of existing highway service wastewater treatment with extremely low carbon-nitrogen ratio, such as the need for additional carbon source / electron donor, high operation cost, poor denitrification effect and high sludge yield.

The invention discloses a composite activated carbon filter material and a preparation process thereof, and belongs to the technical field of water treatment materials. The filter material sequentially comprises a modified coconut shell activated carbon matrix, a bimetallic oxide and a biodegradable functional film from inside to outside, the modified coconut shell activated carbon matrix is prepared by a three-step process of acid leaching modification, microwave activation and nitrogen doping; the bimetallic oxide is Fe-Mn oxide nano particles; the biodegradable functional film is composed of a polyvinyl alcohol / polylactic acid blended film and a complex microbial inoculant, and the complex microbial inoculant comprises a mixed flora of pseudomonas, bacillus and nitrifying bacteria. Through the three-layer composite structure of the coconut shell activated carbon matrix, the bimetallic catalyst layer and the biodegradable film, the synergistic effect of physical adsorption, chemical catalysis and biodegradation is achieved. Through step-by-step superposition of the functional layers, the removal efficiency of complex pollutants such as organic matters, heavy metals, ammonia nitrogen and the like is remarkably improved.

The invention relates to a soil conditioner based on nitrification inhibition and a soil acid control method, and belongs to the technical field of soil conditioning, the soil conditioner comprises the following components by weight: 20-22 parts of humic acid, 32-36 parts of syringic acid-linoleic acid modified porous hydroxyapatite, 4-5 parts of complete nitrifying bacteria, 2-5 parts of syringic acid, and 2.8-4.2 parts of sodium carboxymethyl cellulose. According to the technical scheme, syringic acid and linoleic acid serve as nitrification inhibitors, the nitrification inhibition rate can be effectively increased, ecological niches of ammonia-oxidizing archaea and ammonia-oxidizing bacteria can be reduced through competitive occupation due to addition of complete nitrifying bacteria, the nitrification rate of the ammonia-oxidizing archaea and the ammonia-oxidizing bacteria is inhibited, and N2O emission is reduced. Humic acid and hydroxyapatite can be used as pH buffer substances to regulate and control soil acidity, aluminum toxicity can be relieved, and physical and chemical properties of soil can be improved; meanwhile, the complete nitrifying bacteria can be ensured to have relatively high activity under the condition that the pH is 5.5-6.5.

The application discloses a method for efficient denitrification and decarburization of kitchen waste and sludge co-fermentation anaerobic digestion effluent, which removes part of COD and ammonia nitrogen in the influent sludge by using a first SNAD, and maintains a certain amount of free ammonia of a certain concentration due to the high concentration of ammonia nitrogen and the high pH of the influent, so as to maintain the inhibition effect on nitrifying bacteria and ensure the conversion of part of the ammonia nitrogen into nitrite nitrogen, and the high activity of anaerobic ammonia oxidation bacteria is ensured in the first SNAD by limiting the aeration amount, so that the ammonia nitrogen and nitrite can be further removed; the effluent enters a nitrification tank, and the ammonia nitrogen is nitrified into nitrate nitrogen under the condition of nitrifying bacteria and sufficient aeration amount, and then enters a denitrification tank, and the denitrification process is carried out under the condition of heterotrophic denitrifying bacteria and sufficient carbon source, so that most of the nitrate nitrogen in the system is converted into nitrogen gas and removed, finally, the clear liquid is discharged after being filtered by an MBR membrane in a membrane tank, so as to achieve the purpose of efficient denitrification and decarburization of kitchen waste anaerobic digestion effluent.

The invention relates to the field of sewage treatment, and discloses a sewage treatment method based on desulfurization and denitrification, which comprises the following steps: S1, magnetic flocculation-electro-catalysis pretreatment: adding a polyaluminum chloride-magnetic powder composite flocculant into sewage through a magnetic flocculation reactor to form a magnetic floc, removing suspended matters through a permanent magnetic separation device, and then entering an electro-catalysis oxidation pond, carrying out electrolysis by taking titanium-based lead dioxide as an anode and active carbon fiber as a cathode; and S2, performing synchronous desulfurization and denitrification on the three-dimensional electrode fluidized bed, feeding pretreated effluent into a three-dimensional electrode fluidized bed reactor, filling activated carbon particles as a third electrode, and adding a composite flora of thiobacillus and denitrifying bacteria. The efficient removal of sulfides, total nitrogen, total phosphorus and chemical oxygen demand in the sewage is realized through a multi-stage synergistic process of magnetic flocculation-electrocatalysis pretreatment, synchronous desulfurization and denitrification of a three-dimensional electrode fluidized bed, deep denitrification and dephosphorization of a denitrification filter tank and catalytic ozonation-membrane separation combined deep treatment.

The present invention discloses an on-line wastewater toxicity detection system and method based on nitrifying bacteria biofilm. The present invention uses nitrifying bacteria as a model organism, ensures the concentration of the bacterial community through the biofilm, and at the same time avoids the interference of miscellaneous bacteria through ultraviolet sterilization; by flushing the nutrient solution in the intermittent stage, the biological activity of nitrifying bacteria is protected, providing a highly sensitive and highly stable method and equipment for wastewater toxicity detection. By detecting the concentration of dissolved oxygen in the solutions flowing into and out of the nitrifying bacteria biofilm reactor, the toxicity of wastewater can be evaluated quickly and with high stability. When the wastewater flows through the biofilm reactor, its toxicity can be evaluated in real time through the rate of dissolved oxygen consumption by the nitrifying bacteria biofilm.

The invention discloses an intelligently adjustable micro-ecological base reaction bed, which belongs to the technical field of water treatment, and comprises an ecological base reaction bed, a real-time detection module, a control module, an intelligent aeration module and a fungicide release module, the ecological base reaction bed comprises a reaction bed frame, an MOBR film is attached to the surface of the reaction bed frame, and the reaction bed frame is used for synchronously realizing half-process denitrification and anaerobic ammonia oxidation reaction; the real-time detection module is fixed on the reaction bed frame and is used for monitoring data in the reaction bed and feeding back the data to the control module; the control module is used for realizing remote control, automatic adjustment, fault early warning and dynamic optimization based on real-time detection data; the intelligent aeration module is a nano aeration device and is used for accurately adjusting the aeration rate according to an instruction of the control module, so that the concentration of dissolved oxygen is maintained at 0.2-0.5 mg / L; and the microbial agent release module comprises a plurality of microbial agent slow-release adding devices, is mounted near the intelligent aeration module on the lower layer of the reaction bed frame, and is used for dynamically adding nitrifying bacteria or denitrifying microbial agents according to instructions of the control module.

The invention provides a sewage enhanced nitrogen and phosphorus removal process based on microbial community interaction, and belongs to the field of sewage treatment. The method comprises the steps of sewage pretreatment, anaerobic phosphorus release, anoxic denitrification, aerobic nitrification phosphorus uptake and the like, an anaerobic-anoxic-aerobic three-section reactor is adopted, and proper ecological nichs are provided for phosphorus-accumulating bacteria, denitrifying bacteria and nitrifying bacteria by accurately regulating and controlling parameters such as dissolved oxygen, hydraulic retention time and carbon nitrogen ratio of each section, so that the phosphorus-accumulating bacteria, the denitrifying bacteria and the nitrifying bacteria can be efficiently degraded, and the phosphorus-accumulating bacteria, the denitrifying bacteria and the nitrifying bacteria can be efficiently degraded. The competition problem of biological nitrogen removal and biological phosphorus removal is effectively relieved, the nitrogen and phosphorus removal efficiency is remarkably improved, the total nitrogen removal rate reaches 85% or above, and the total phosphorus removal rate exceeds 90%; straw-based modified charcoal is adopted as a carrier, so that the loading capacity and activity of functional flora are improved, and flora interaction is enhanced; meanwhile, resource utilization of agricultural wastes is realized, and the method has remarkable environmental benefits and economic advantages.

The invention relates to the technical field of soil carbon improvement, and discloses a composite biological stimulant for tropical rainforest soil carbon improvement and a preparation method and application thereof, the composite biological stimulant is obtained by mixing a microbial preparation, an immobilized carrier, porous biochar, an inorganic mineral compound, a slow release fertilizer, a soil conditioner and a plant symbiotic inoculant; the microbial preparation comprises one or more of lactic acid bacteria, nitrifying bacteria, phosphate solubilizing bacteria, nitrogen-fixing bacteria and arbuscular mycorrhizal fungi; the inorganic mineral compound comprises feldspar, apatite and montmorillonite; the slow-release fertilizer comprises biochar, humic acid and phosphate; the soil conditioner comprises biological colloid prepared from seaweed extract and natural plant fiber prepared from straw and palm fiber; the plant symbiotic inoculant comprises rhizobium and / or endophyte. According to the composite biological stimulant, the soil carbon fixing capacity of the tropical rainforest is remarkably improved, the soil void structure is improved, and the plant growth capacity is enhanced.

The invention discloses a water quality conditioning agent based on plant extracts and a preparation method of the water quality conditioning agent, and belongs to the technical field of aquaculture. The water quality conditioning agent is prepared from the following raw materials in parts by weight: 14 to 18 parts of fructus schisandrae chinensis extract, 4 to 5 parts of fructus mume extract, 10 to 13 parts of rhizoma coptidis extract, 8 to 10 parts of glucosamine, 5 to 8 parts of chitosan, 3 to 6 parts of nitrifying bacteria, 2 to 4 parts of thiobacillus thiooxidans and 3 to 6 parts of zeolite powder. Through a four-dimensional synergistic mechanism of'plant active ingredient-chitosan slow release-microbial immobilization-zeolite adsorption ', the pH value, ammonia nitrogen, nitrite, hydrogen sulfide and bottom mud COD in tail water can be reduced and the dissolved oxygen amount can be increased under the condition of one-time addition, which indicates that the water quality regulator has a good effect of improving water quality.

A fast biofilm-forming electrospinning carrier, and its preparation method and application. The present invention belongs to the field of sewage biological treatment. The purpose of the present invention is to solve the technical problem that the existing methods cannot achieve the sustained release of AHLs by directly adding AHLs or simply fixing AHLs on the carrier surface. The method of the present invention: S1: dissolving a polymer and AHLs in an organic solvent to obtain a mixed solution; S2: electrospinning the mixed solution to obtain an electrospinning carrier with fast biofilm formation. In the obtained electrospinning carrier, AHLs are wrapped or attached to fibers. The present invention can accelerate the formation of biofilms on the carrier surface, promote the enrichment of nitrifying bacteria, and enhance the degradation of nitrogen-containing pollutants in water. Compared with traditional biofilm carriers, the present invention can shorten the biofilm formation and acclimation cycle, so that the sewage treatment unit used can meet the relevant effluent standards earlier.

The invention discloses a device and a method for realizing double-short-range coupling anaerobic ammonia oxidation sewage denitrification by combining biologically-enhanced carbon capture with a two-stage membrane aeration bio-membrane reactor. A high-load activated sludge device and an anaerobic fermentation tank are used for carbon source extraction, and a carbon source is provided for a subsequent anoxic tank. The two-stage MABR system adopts a hollow fiber membrane to perform bubble-free membrane aeration, and oxygen is diffused to form an oxygen concentration gradient, so that the surface of the membrane is divided into an oxygen-rich region and an oxygen-poor region. In the aerobic section, the dissolved oxygen gradient of a biological membrane is regulated and controlled to construct a nitrifying bacterium and anaerobic ammonium oxidation bacterium function partition, so that short-cut nitrification coupled anaerobic ammonium oxidation synergistic denitrification is realized; in the anoxic section, nitrate nitrogen and part of ammonia nitrogen are reduced and COD (Chemical Oxygen Demand) is removed through the synergistic effect of short-cut denitrification and anaerobic ammonia oxidation and synchronous nitrification and denitrification on the surface of the membrane. The two-stage MABR synergistic carbon capture device denitrification system constructed by the method realizes efficient and stable deep denitrification, reduces the addition of a carbon source and the yield of sludge, and saves energy consumption.

The invention relates to a CdS / denitrifying bacteria hybrid system as well as a construction method and application thereof, and relates to the technical field of water nitrate pollution treatment. Comprising composite denitrifying bacteria, Zn-doped CdS heterojunction nanoparticles loaded on the surface of the composite denitrifying bacteria, and an electronic mediator anchored at the interface of the heterojunction nanoparticles and a flora, the composite denitrifying bacteria are prepared from Stutzeria balearica CXK-1 and Stutzeria balearica GUA-1 according to the volume ratio of the Stutzeria balearica CXK-1 to the Stutzeria balearica GUA-1 to the Stutzeria balearica GUA-1 to the Stutzeria balearica GUA-1 being 1 to (0.8 to 1.2). The hybrid system can utilize photoelectrons to drive microbial denitrification under an illumination condition, so that the reduction of nitrate radicals is realized, and the effect of water treatment is achieved.

The invention relates to the technical field of sewage treatment, and discloses a sewage biotoxicity detection system. The system comprises a reaction module, a detection module and a data processing module, the reaction module, the detection module and the data processing module are connected in sequence; the reaction module is used for providing a metabolic environment for the sewage to be detected and nitrifying bacteria; the detection module is used for detecting activity data in a metabolic environment; the activity data comprises at least one of ammonia nitrogen concentration, microbial fuel cell current and active oxygen fluorescence signal intensity; and the data processing module is used for determining the toxicity of the sewage to be detected based on the activity data. According to the present invention, with the nitrifying bacteria as the indicator organism of the sewage toxicity, the accurate evaluation of the sewage biological toxicity is achieved based on the detected multi-dimensional activity data such as the ammonia nitrogen concentration, the microbial fuel cell current, the active oxygen fluorescence signal intensity and the like.

The utility model discloses a water distributor for integrated sewage treatment equipment. The water distributor comprises a main water inlet pipe, a water distribution main pipe and a plurality of branch pipe groups, the main water inlet pipe extends in the Z direction, and the bottom of the main water inlet pipe is communicated with the water distribution main pipe; the water distribution main pipe is of a square frame structure formed by mutually connecting two transverse pipes and two longitudinal pipes; the plurality of branch pipe groups are sequentially arranged in the water distribution main pipe square frame along the X direction or the Y direction, each branch pipe group comprises a branch pipe and a plurality of homogeneous dispersion pipes uniformly distributed on the branch pipe, two ends of the branch pipe are communicated with the water distribution main pipe, the upper end of each homogeneous dispersion pipe is communicated with the branch pipe, and the lower end of each homogeneous dispersion pipe is closed; and water outlet holes are annularly distributed in the outer wall of the homogeneous dispersion pipe. Sewage and denitrifying bacteria in the tank can be fully fused, so that the reaction rate of the denitrification process is greatly improved, and the reaction time is shortened.

This utility model discloses an oxygen denitrification treatment device for aquaculture water, including a treatment tank. The treatment tank is equipped with a filtration zone, an aerobic biological zone, an anoxic biological zone, and a degassing zone. The filtration zone is filled with quartz sand filter media. The aerobic biological zone contains biological packing material and has a jet aeration ring on its outer wall, which is connected to an ozone-oxygen mixture generator. The anoxic biological zone is filled with porous ceramsite packing material. The degassing zone has a guide plate inside. In this utility model, the aquaculture water can be filtered for suspended particulate matter through the quartz sand filter media. The jet aeration ring can introduce ozone-oxygen mixture gas, which, together with the honeycomb biological packing material, can complete ammonia nitrogen oxidation. The denitrifying bacteria on the surface of the porous ceramsite packing material can convert nitrates into nitrogen gas. Finally, the gas is separated by the guide plate, and the purified water can be discharged from the outlet. Thus, through the above structure, efficient denitrification, oxygenation, and purification of aquaculture water can be achieved, improving water quality treatment efficiency and stability.

The invention belongs to the technical field of stripping agents, and particularly relates to a slime stripping agent and a preparation method and application thereof. The slime stripping agent disclosed by the invention is prepared from dodecyl dimethyl benzyl amine bromide, dichlorophenol, isothiazolinone, polyoxyethylene alkylolamide, hydroxyethylidene diphosphonic acid, dimethylformamide and sodium dodecyl benzene sulfonate. Through the combined action of the specific components, the slime stripping agent has good stripping capacity and can rapidly permeate and disintegrate slime, dirt and biological membranes attached to the surface of equipment, the cleanliness and operation efficiency of the equipment are recovered, and equipment blockage and performance reduction caused by slime accumulation are effectively avoided; meanwhile, the high killing effect on sulfate reducing bacteria and nitrifying bacteria is achieved.

The application provides a biological denitrification treatment method for low-carbon-nitrogen-ratio wastewater, and utilizes single-stage nitrification and denitrification to treat ammonia-nitrogen wastewater in a one-piece submerged biological filter (SBAF reactor). The method comprises the following steps: sludge inoculation, sludge biofilm formation, system starting, and stable operation. In the sludge inoculation, sludge meeting the microbial community distribution is selected. In the system starting process, the microbial community distribution in the sludge in the one-piece submerged biological filter (SBAF reactor) is used as the symbol of successful system starting. During the input of the high-concentration ammonia-nitrogen wastewater to be treated, the aerobic nitrifying bacteria and the anaerobic denitrifying bacteria can more stably meet the above microbial community distribution, the system has better stability, the stable operation time is longer, the sludge does not need to be replaced for restarting during the stable operation, the continuous operation time is longer, can reach at least 250 days or more, and the frequency of replacing the sludge for restarting is reduced.

The present application relates to sewage treatment technical field, especially a kind of composite biological additive for high-nitrogen sewage advanced treatment.The present application obtains a high-quality fermentation liquor by using specific enzymolysis sequence enzymolysis agricultural derivative, wherein it contains more reducing sugar and organic small molecule acid, and COD equivalent is extremely high, is 2~5 times of the carbon source COD used by conventional sewage plant, and has excellent effect when used as biological carbon source for sewage treatment;Meanwhile, the fermentation liquor is compounded and adjusted with AOB bacterial agent to obtain composite biological additive, the composite biological additive can provide sufficient high-quality nutrient ingredients for denitrifying bacteria, and also inoculate AOB bacteria for sewage treatment system, so that AOB bacteria in the system gradually develop into main nitrosation bacteria group, and have excellent enhanced denitrification effect on high-nitrogen sewage, and have wide application prospect.

This application relates to the field of wastewater treatment, specifically disclosing a sulfur autotrophic denitrification process; including the following specific steps: S1: After adjusting the pH of calcium nitrate tetrahydrate solution and ammonium dihydrogen phosphate solution to alkaline, triethanolamine is added dropwise to the calcium nitrate solution, followed by the addition of ammonium dihydrogen phosphate solution to form a reaction solution. The pH of the reaction solution is maintained at alkaline, and the reaction is heated. After filtration, washing, and drying, hydroxyapatite powder is obtained. The hydroxyapatite powder is mixed with a foaming agent, foam stabilizer, and adhesive, pressed into shape, and sintered to obtain a porous ceramic carrier; S2: The porous ceramic carrier, ground inorganic energy material, and denitrifying bacteria are added to the wastewater to be treated, mixed, and subjected to aeration treatment. The inorganic energy material is replenished periodically to complete the process. The sulfur autotrophic denitrification process used in this application has high denitrification efficiency, and no replenishment of denitrifying bacteria is required during the process.

The invention belongs to the technical field of sewage treatment, and discloses a rapid denitrification device for micro-polluted river water, which comprises a denitrification mechanism, the denitrification mechanism comprises a denitrification tower, and an aerobic nitrification layer, an anoxic denitrification layer and an adsorption insurance layer are respectively arranged in the denitrification tower from top to bottom. The system has the following beneficial effects that river water enters the denitrification tower and sequentially flows through the aerobic nitrification layer, the anoxic denitrification layer and the adsorption insurance layer, the aerobic nitrification layer is aerated to supply oxygen, and nitrifying bacteria accelerate conversion of ammonia nitrogen into nitrate; denitrifying bacteria in the anoxic denitrification layer convert nitrate into nitrogen, and the two layers synergistically improve the efficiency; the adsorption insurance layer is stable in residual substance adsorption effect. The porous partition plate water permeable holes are smaller than the filler particle size, smooth circulation of water flow is guaranteed, and rapid and efficient denitrification is achieved.

This invention discloses a denitrification biological carrier, its preparation method, and its application. The raw materials for preparing the denitrification biological carrier include an iron agent, modified activated carbon, polycaprolactone, sodium dodecyl sulfate, and a hydrogel binder. The modified activated carbon is 3-chloro-2-hydroxypropyltrimethylammonium chloride-modified activated carbon. The iron agent not only removes dissolved oxygen from water but also reduces total nitrogen in wastewater. The modified activated carbon improves its adsorption affinity for nitrogen-containing compounds. Polycaprolactone serves as a slow-release carbon source for denitrification, exhibiting high carbon source utilization. Sodium dodecyl sulfate, through its foaming properties, together with the modified activated carbon, iron agent, and polycaprolactone, forms a high-mechanical-strength, rough, and porous biological carrier structure, providing excellent attachment conditions for heterotrophic denitrifying bacteria. Therefore, applying the denitrification biological carrier of this invention to wastewater treatment systems can achieve good nitrogen removal efficiency and economic benefits.

1. The name of the design product: aquarium filter material (gear bacteria breeding column). 2. The use of the design product: purification, filtration, adsorption and cultivation of nitrifying bacteria for aquariums. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The application discloses a method for efficiently removing sulfonamide antibiotics by flocculent sludge domestication, and belongs to the technical field of sewage treatment. The method greatly improves the removal rate of sulfonamide antibiotics by adjusting the culture conditions of activated sludge in a traditional activated sludge system. The method can effectively remove antibiotics, has the characteristics of simple operation, low treatment cost and large-scale operation, and the removal of pollutants is mainly realized by sludge adsorption and microbial degradation. The important functional bacteria, nitrifying bacteria, in the activated sludge treatment process can improve the removal efficiency of antibiotics including antibiotic drugs through co-metabolism while completing nitrification, so that the functional bacteria can be enriched by inoculating activated sludge and domesticating the sludge into flocculent form, and the functional bacteria are used to efficiently remove sulfonamide antibiotics.

This invention discloses a heterotrophic-aerobic denitrifying bacterium C-3 and its applications. The heterotrophic-aerobic denitrifying bacterium C-3 is deposited at the China General Microbiological Culture Collection Center (CGMCC), classified as *Brevundimonas naejangsanensis*, with accession number CGMCC No. 31729, and deposited on August 23, 2024. The heterotrophic-aerobic denitrifying vesicular *Brevundimonas naejangsanensis* strain C-3 provided by this invention exhibits excellent nitrate nitrogen removal efficiency (100%) under aerobic conditions using sodium acetate as a carbon source, a C / N mass ratio of 10, a pH of 7, a temperature of 30℃, and a rotation speed of 200 r / min. In actual aquaculture wastewater, it demonstrates excellent removal effects on nitrate nitrogen, nitrite nitrogen, ammonia nitrogen, and total nitrogen. Furthermore, this strain exhibits high safety and has significant application value in treating aquaculture wastewater.

The invention relates to the technical field of sewage treatment, in particular to a water body biotoxicity early warning system based on self-sustaining of denitrifying microorganisms. The early warning system comprises an early warning device, an early warning filtering system located at the front end of the early warning device, a nitrate nitrogen monitor located at the rear end of the early warning device, a carbon source storage tank used for storing a carbon source and a nitrate nitrogen storage tank used for storing nitrate nitrogen. The nitrate nitrogen monitoring instrument is used for monitoring the change of the concentration of nitrate nitrogen, so that the toxicity detection and early warning of inlet water are realized. The water body biotoxicity early warning system is arranged based on denitrifying bacteria, through the biofilm filler, the early warning system is high in impact resistance and better in stability in the use process, and meanwhile, the system is more stable and reliable in the use process in cooperation with a corresponding filtering-backflushing system, a filler stirring and flushing system and a parallel system; and the device is convenient to use and maintain, is more suitable for actual production, and has wide application and popularization prospects.

The application provides a method and device for treating wastewater by combining a suspended filler floating bed and a soil infiltration system, and the method comprises the following steps: S1, breeding wastewater is treated by the suspended filler floating bed after passing through a hydrolysis tank, and effluent is treated in a sedimentation tank, and wastewater of the suspended filler floating bed is returned to the suspended filler floating bed for secondary treatment; S2, wastewater of the sedimentation tank is returned to the hydrolysis tank; and S3, effluent of the sedimentation tank is introduced into a soil underground infiltration system. The system provided by the application is beneficial to further strengthening the effects of denitrification, phosphorus removal and removal of suspended solids, and solves the problems of low dissolved oxygen content of influent and wastewater in the system, which is not conducive to the growth of nitrifying bacteria, and low effective volume utilization rate. In the use process, the filler does not need to be replaced, and mechanical aeration equipment does not need to be added, and the system has the advantages of low investment cost, no need to add chemical agents for treatment, obvious effect, not easy to breed mosquitoes, simple operation and management, and beautification of the environment, and has remarkable ecological and economic benefits for promoting deep purification of breeding wastewater.

This utility model discloses an aerobic mixed liquor nitrification and deoxygenation treatment device, including a first nitrification tank. A nitrite-containing bacteria storage tank is located at the top of the first nitrification tank. An inlet pipe is located at the top of one side of the first nitrification tank, and one side of the inlet pipe extends into the interior of the first nitrification tank and is equipped with a sludge-water separation chamber. Through the cooperation of the inlet pipe, sludge discharge pipe, screen, and sludge-water separation chamber, this utility model can separate the sludge mixed in with the mixed liquor during its inflow, while also facilitating the return of the sludge to the treatment tank. Then, through the cooperation of a second discharge pipe, a third pump, a pump installation chamber, a distribution plate, a distribution pipe, a heat dissipation chamber, and a fan, the mixed liquor can be continuously circulated and cooled during the nitrification reaction, thereby maintaining the mixed liquor at 20-30°C to preserve the activity of nitrite-containing bacteria and nitrifying bacteria in the mixed liquor and ensure reaction efficiency.