1673 results about "Nitrifying bacteria" patented technology

The invention relates to a method for producing a high-efficiency composite microbial agent by using livestock liquid dung, and belongs to the technical field of environmental microbiology and agricultural science. The method comprises the following steps: diluting livestock liquid dung or livestock feces with water; after hydrolysis and acidification treatment, performing standing and settlement for desilting; mixing a supernatant obtained with a photosynthetic bacterial solution for anaerobic culture; using the mixed solution as a base liquid and stepwise adding lactobacilli, saccharomyces, bacillus thuringienesis and actinomycete into the base liquid in a proportion; and after anaerobic culture and aerobic treatment, finally filling the mixture into a biological fluidized bed for further treatment to obtain the high-efficiency composite microbial agent. The microbial agent is rich in the photosynthetic bacteria, the lactobacilli, the saccharomyces, the actinomycete, the bacillus thuringienesis, azotobacter, nitrobacteria, denitrifying bacteria and other beneficial organisms, and be widely used in agriculture, livestock breeding, fresh water aquaculture, organic sewage treatment, microbial deodorization, and other aspects. The technology is suitable to be used by various livestock farms (raisers) for liquid dung treatment.

The invention discloses a microbe complexing agent with high efficiency and method using thereof for processing municipal wastewater. The microbe complexing agent contains 5-20 shares of nitrifying bacteria, 5-20 shares of denitrifying bacteria, 5-15 shares of bacillus brevis, 5-20 shares of photosynthetic bacteria, 1-20 shares of saccharomycete, each grame culture medium of single species bacteria contains 1.0-3.5 hundred million numbers of bacteria. The invention adopts improved bio-contact oxidation process with a microbe complexing agent with high efficiency as main body, adopting soft assembly complexing biology stuff as biology carrier, increaing anoxia step in a integral SBQ biochemistry treating pool to make hydrolysis and good oxygen oxidize as an integer, nitrifying and denitrifying finished in a pool, removing effectively ammonia, nitrogen and phosphor via aeration regulation and regulation of microbe reaction process. The microbe complexing agent is in characterized in high efficiency and no pollution, the waste water processing method with low cost, less invest, low operation cost, and the method has strong adaptability, less mud product, having better economy and social benefit.

The present invention relates to a wastewater treating system and method for removing pollutants of carbon, nitrogen, phosphorous, comprising: a first reactor contains methanogenic bacteria, PAO and denitrifying bacteria for eliminating the organic carbon in denitrification by denitrifying bacteria, adsorbing partial organic carbon and releasing phosphate by PAO and; metabolizing organic carbon to form methane gas by methanogenic bacteria; a second reactor disposed at the rearward of first reactor, second reactor treats the effluent from first reactor by denitrifying bacteria and PAO, denitrifying bacteria is able to denitrifying the nitrate and PAO is able to process the phosphorous releasing; a third reactor disposed rearward of second reactor, which comprises PAO, heterotrophic bacteria and nitrifying bacteria, wherein PAO is applied for phosphorous accumulating process, the heterotrophic bacteria is applied for carbon removal process, the nitrifying bacteria is applied for nitrifying process; and a membrane separation reactor disposed inside or rear of third reactor to separate various microorganisms and the treated effluent. The present invention applies methanogenic bacteria in the system for decreasing the amount of waste sludge efficiently and reduce power consuming.

The invention discloses a enrichment method of nitrifying organism. The method adapts discontinuous activated sludge process by gradually improving ammonia nitrogen ph indicator in nutrient fluid to enrich. The main component of the enrichment nutrient fluid is inorganic salt comprising microelement Fe, Mg, Na, K and amortization liquor, in which inceptive concentration of NH(4)(+-N) is 100mg/L to 200mg/L, final concentration is 500mg/L to 1200mg/L and COD is less than or equal to 200mg/L. The method can restrain evidence the growth of sundry bacterium such as carbonizing bacterium, is propitious for nitrifying organism to become ascendency bacterium and resist more and more high ammonia nitrogen concentration, and finally disposing concentration of the ammonia nitrogen wasted water is up to 1200mg/L and high concentration ammonia nitrogen in wasted water can be reduce to less 10mg/L.

A method for the phylogenetic identification of indigenous anaerobic denitrifying bacterial species in an oil well is described. The method avoids the process of enrichment of microbial samples.

The invention discloses a preparation method and an application of immobilized bacteria for improving the denitrification efficiency of a constructed wetland. 1. The preparation steps are as follows: 1) screening and culturing aerobic denitrification strains which are screened out of the constructed wetland in a thermotank; 2) selecting aerobic denitrification bacteria in slope conservation, inoculating into a sterile enrichment culture medium and aerating to get bacterial suspension; 3) using sodium alginate-polyvinyl alcohol to embed so as to get the immobilized aerobic denitrification bacteria; and 4) taking pellets of the aerobic denitrification bacteria out of a refrigerator, washing with double distilled water or physiological saline, soaking in the physiological saline, and aerating to activate the immobilized pellets. 2. An application of the immobilized bacteria in the constructed wetland is as follows: filling the immobilized pellets into a simulation column of the constructed wetland for performing waste water denitrification treatment. The preparation method of the immobilized bacteria is simple, the production cost is low, the application in the industrial waste water treatment is easy, the denitrification efficiency is high, the accumulation amount of nitrite nitrogen is low, and the service life is long.

The invention relates to a compound microecological preparation used to purify aquaculture water in the field of aquiculture, a preparation form and a preparation process thereof; the preparation is composed of photosynthetic bacterium, bacillus subtilis, denitrifying bacterium, coagulant aid and auxiliary components; the preparation form is composed of an inner layer and an outer layer, wherein, the outer layer comprises bacillus subtilis, denitrifying bacterium and coagulant aid, and the inner layer comprises solid photosynthetic bacterium particles. The invention breaks through the prior theory that people keeps using nitrite bacteria and nitrobacteria to solve the problem of the accumulation of ammonia nitrogen and nitrite in the aquaculture water in the control of aquaculture water, introduces an advanced SHARON theory in wastewater treatment, integrates the characteristics of the aquaculture water quality, purifies the aquaculture water with denitrifying bacteria strains as the main bacteria strains, can quickly and effectively reduce the content of NH3 and NO2- in the aquaculture water, is not inhibited by the concentration of the NO2-, and effectively reduces the oxygen consumption in the aquaculture water.

A method for co-immobilizing anammox bacteria-short-range nitrifying bacteria and its application belong to the technical field of sewage treatment, using polyvinyl alcohol, sodium alginate and activated carbon as embedding agents to anaerobic ammonium oxidizing bacteria and short-range nitrifying bacteria Layered embedding and fixation, firstly mix equal volumes of anammox sludge and embedding agent uniformly, get anaerobic ammonium oxidation gel pellets after fixation, and then put them into the mixed solution of short-range nitrifying bacteria and embedding agent In the process, anammox-short-range nitrifying bacteria gel pellets were obtained after fixation, and were directly added to the autotrophic denitrification reactor after activation. The present invention uses immobilized microbial technology to co-immobilize anammox bacteria and short-range nitrification bacteria on the same carrier, and stably enrich high-activity anammox and short-range nitrification sludge, so that the biological efficacy of the two bacteria can be coordinated. , to realize the coordinated operation of short-range nitrification reaction and anammox reaction.

The invention discloses a method for treating high-salinity high-concentration ammonia-nitrogen wastewater, characterized by: adopting sequencing batch reactor, accumulating nitrification sludge by alternately increasing the concentration of a nutrient solution or ammonia-nitrogen concentration, and domesticating to obtain high salinity-resisting high ammonia nitrogen-resisting nitrobacteria. According to the invention, the nitrobacteria can treat high-salinity wastewater having ammonia-nitrogen concentration of 500mg/L and having the salt content of no larger than 35g/L to make the effluent have the ammonia-nitrogen concentration of no larger than 10mg/L, thus the effluent achieve national level of discharging standard; the method has good effect on treating high-salinity high ammonia-nitrogen wastewater in chemical plant, leather factory, oil refinery and the like, can overcome the shortage that the nitrobacteria cannot simultaneously treat high-salinity and high ammonia-nitrogen wastewater existing in the prior art, and has important economic benefits and social benefits.

The invention discloses a device and method for nitrogen and phosphorus removal treatment by denitrification on high-ammonia nitrogen anaerobic ammonia oxidation effluent and domestic sewage, belonging to the field of biological sewage treatment. The device comprises a high-ammonia nitrogen inflow water tank, an integrated partial nitrification and anaerobic ammonia oxidation reactor, a sedimentation tank, a domestic sewage inflow water tank, a denitrification nitrogen and phosphorus removal reactor and an effluent water tank sequentially connected in series. The method comprises the following steps: in the integrated reactor, effectively removing nitrogen of the high-ammonia nitrogen wastewater through the effect of partial nitrification and anaerobic ammonia oxidation; in the denitrification nitrogen and phosphorus removal reactor, performing anaerobic phosphorus release by phosphorus-accumulating bacteria by use of an organic carbon source in the domestic sewage, and performing hypoxia denitrification phosphorus removal by use of nitrate nitrogen in the anaerobic ammonia oxidation effluent; and finally, further absorbing phosphorus at the aerobic zone in accompany with the nitrification effect generated by the nitrification bacteria. According to the method, the nitrogen removal by anaerobic ammonia oxidation and phosphorus removal by denitrification are coupled into a biological nitrogen and phosphorus removal system for sewage, the nitrate nitrogen generated in the anaerobic ammonia oxidation process is effectively utilized, and the oxygen consumption and energy consumption are greatly reduced.

The invention discloses a method and a device for simultaneously carrying out wastewater treatment and power generation by using a photocatalysis associated microbial fuel cell technology. The device takes conductive glass coated with an n-type semiconductor photocatalyst film as an outside wall of a cathode chamber and takes conductive glass coated with an n-type semiconductor photocatalyst film as an outside wall of an anode chamber, a conductive filler respectively connected on the outside walls of the cathode chamber and the anode chamber is used as an electrode of a microbial fuel cell. The conductive filler is a carbon fiber rope for microbial growth. Denitrifying bacteria and nitrifying bacteria are respectively cultivated at the cathode and anode of the microbial fuel cell, and mainly used for the degradation of organic matters and the removal of ammonia nitrogen. Under the irradiation of ultraviolet light, electrons flow from the anode to the cathode, so that the anode has strong oxidability, and the cathode has strong reducibility. In the invention, the functions of microbial fuel cells and a photocatalysis technology are mutually associated, so that the wastewater treatment cost is low, and the generating capacity is large.

The invention relates to a microorganism immobilization method. The method comprising the following steps of: taking a sodium alginate solution containing dense suspending microorganisms and nano calcium carbonate as disperse phases, taking atoleine or vegetable oil as a disperse medium, and taking Span85 as a surfactant; forming droplets through agitation and emulsification; then preparing calcium alginate bead gel embedding the microorganisms through a gel initiator of glacial acetic acid; forming a polycation-polysnion complexing membrane through the reaction and cross linking of the bead gel and a chitosan solution; and finally forming a sodium alginate-chitosan microcapsule embedding the microorganisms. The method has mild conditions in the preparation process, simple requirements on apparatuses and equipment, easy mass production, good maintenance of the activity of the microorganism cells in the preparation process, and lower cell leakage rate. Thus, the technology is suitable for the immobilization embedding of a plurality of microorganisms such as yeast, bacillus coli, lactic acid bacteria, nitrobacteria, etc., thereby realizing high-density and large-scale microorganism culture.

The invention discloses a preparation method of compound microorganisms and a recombination enzyme solid agent which can degrade the ammonia- nitrogen and nitrite in the aquicultural water environment. The invention uses universal liquid medium to culture bacillus subtilis, nitrifying bacteria, photosynthetic bacteria, denitrifying bacteria, yeast, actinomyces etc six under different temperatures. Then the culture liquid is centrifugally separated and then the bacteria body is collected. The bran is added into the bacteria body as an adsorbent. The mixture of the bacteria body and the bran is boiled and dried on a fluidized bed. And the freeze-dried powder of compound microorganisms is obtained. Then proteases, lipase, and amylase are added to make the compound microorganisms and the recombination enzyme solid agent. The invention is put into the aquicultural water that needs to be dealt with and becomes nitro-nitrogen by the decomposition of proteases, lipase, and amylase and the ammonification of bacillus subtilis. Next, the nitro-nitrogen becomes nitrogen which is released into the air. So the invention can take effect quickly on completely degrading the ammonia- nitrogen and nitrite in the aquicultural water.

A process for treating nitrogenous wastewater contains an autotrophic denitrification reaction, a heterotrophic denitrification reaction and a COD removal reaction simultaneously and mixedly taking place in a single reactor. The nitrification reaction is caused by nitrifying bacteria, in which ammonium is oxidized into nitrite. The autotrophic denitrification reaction is caused by autotrophic denitrifying bacteria, in which ammonium used as electron donor and nitrite used as electron acceptor are converted into nitrogen gas and nitrate. The heterotrophic denitrification reaction is caused by heterotrophic denitrifying bacteria, in which nitrate and COD are consumed. It is not necessary to build two separate reactors for aerobic nitrification and anaerobic denitrification, thereby effectively reducing the fabrication and operation cost.

The invention discloses a microorganism concrete brick and a preparation method thereof. The microorganism concrete brick is formed by mixing cement, gravel, sawdust (or straw scraps, scraps of paper, and the like), mixing mixed microorganism with water according to a certain proportion, forming by a mold and then naturally hardening, wherein the mixed microorganism comprises six floras including bacillus, nitrobacteria, photosynthetic bacteria, saccharomycetes, lactobacillus and actinomycetes. The microorganism concrete brick has excellent water quality purifying function, can continuously play roles for a long time, and is widely applicable to purify various water qualities such as rivers, lakes, canal water, swimming pools, fishponds and the like.

The invention discloses domestic garbage harmful microorganism elimination deodorization liquid and a preparation method thereof. The domestic garbage harmful microorganism elimination deodorization liquid comprises probiotics components as follows: photosynthetic bacteria accounting for 11 to 17 parts, bacillus accounting for 9 to 13 parts, lactic acid bacteria accounting for 24 to 31 parts, saccharomycete accounting for 17 to 23 parts, nitrifiers accounting for 8 to 12 parts, acidithiobacillus accounting for 8 to 12 parts and ray fungi accounting for 10 to 15 parts. Meanwhile, the invention further discloses the preparation method of the domestic garbage harmful microorganism elimination deodorization liquid. The domestic garbage harmful microorganism elimination deodorization liquid performs self symbiotic propagation to generate group bacteria containing a plurality of beneficial bacteria that can achieve co-existence, co-prosperity and synergism; propagation can produce acid, so that the pH value is decreased to reach 3.6 to 4.8; and various beneficial derivatives for harmful microorganism elimination deodorization are generated. Various harmful microorganisms in domestic garbage can be inhibited so as to be eliminated, so that the generation of malodorous gas can be solved from the source of malodorous gas (hydrogen sulfide, ammonia gas and the like). The harmful microorganism elimination deodorization effect is good, the speed is high, and the suitability is strong. Meanwhile, toxic and harmful substances are converted to nontoxic and harmless substances facilitating growth and absorption of plants during the decomposing process of domestic garbage.

The invention relates to a biological nitrite-degrading agent. It is characterized in that it comprises following steps: culturing bacillus subtilis and nitration bacteria separately, solidifying culturing product with centrifugal, absorption and alginate-calcium chloride embedding, mixing and granulating to get said product. The invention is characterized in that it employs special fermentation method to make product posses a large quantity of bacteria and active physiological metabolism; it makes use of composite solidifying method to make various elements be fixed on special carrier for effective and stable wastewater treatment.

The invention discloses a composite bacteria-algae preparation, which consists of nitrifying bacteria, bacillus licheniformis, scenedesmus obliqnus and selenastrum capricornutum, wherein the volume ratio of the nitrifying bacteria to the bacillus licheniformis to the scenedesmus obliqnus to the selenastrum capricornutum is 1-2: 1-3:1-3:1-3, and the microbe inoculation amount per milliliter is 8*106CFU. The composite bacteria-algae preparation also can be prepared into a globular immobilized preparation by adopting a known embedding method, using the nitrifying bacteria, the bacillus licheniformis, the scenedesmus obliqnus and the selenastrum capricornutum as embedding bacteria and using polyvinyl alcohol, sodium alginate, active carbon, silicon dioxide and calcium carbonate as embedding agents. The bacteria, algae and immobilization materials used by the composite bacteria-algae preparation have no toxic or side effect on aquaculture animals, and can effectively purify the aquaculture waste water; the bacteria and the algae can inhibit the growth of harmful pathogenic bacteria to a certain degree; an immobilization method has simple operation; the immobilized efficient bacteria-algae microbial bactericide is easy to store and transport; and the immobilization materials are economic and practical and easy to recycle.

The invention relates the microorganism agent used to control oil field sulfate-reducing bacteria and its application. The invention can used to control oil field secondary depletion sulfate-reducing bacteria and microorganism. The water outlet system of secondary depletion technology comprises sulfate-reducing bacteria, H2S and sulphide, which can cause corrosion and pollution of sulphide. Microorganism agent comprises denitrifying bacteria culture, nutrescin and synergic nutrescin. 0.01-1wt% denitrifying bacteria culture, 5-100g/L culture and 10-200ppm synergic nutrescin. Microorganism agent can avoid SRB growing, SRB producing H2S, and removing sulphide. At the same time, metabolite can improve recovery efficiency.

The invention discloses a method for purifying mariculture wastewater by utilizing a compound microbial inoculant. The method comprises the following steps: (1) screening the compound microbial inoculant which consists of photosynthetic bacteria, nitrobacteria, denitrifying bacteria, candida utilis, lactobacillus acidophilus and bacillus; (2) activating the compound microbial inoculant by utilizing a culture medium: directly adding the strain into the culture medium based on 1% of inoculation amount (w/v), and activating the strain by a shaker at the temperature of 37 DEG C with the speed of 150rpm (revolution per minute) for 24 hours so that the total content of microbes is 7*10<8>-9*10<8>CFUs (colony forming units)/mL; and (3) adding the activated compound inoculant into the treated aquaculture wastewater, wherein the compound inoculant accounts for 0.06%-0.12% of the total weight of the aquaculture wastewater. The method has the advantages of no toxicity and harm on aquaculture animals, simple process, fast organic matter degradation speed, obvious effect, no drug residues and no secondary pollution. Therefore, the method has economic feasibility and is applicable to popularization and application to coastal aquaculture industry.

Water treatment equipment for recirculating aquaculture contains: at least one aquaculture tank, an aeration unit, at least one water circulating tank, a plurality of membrane filtering units and a water returning unit. Each aquaculture tank includes plural connecting plate units and a flowing cup; the aeration unit includes a plurality of air supply pipes. Each water circulating tank includes a nitration reacting chamber and an accommodating chamber, and the nitration reacting chamber has a flow tube outwardly extending to the flowing cup and a nitrifying bacteria layer fixed therein. Each membrane filtering unit is a circularly flat membrane and includes a water pipe and plural disc-shaped film bags, wherein each film bag has two filtering films communicating with the water pipe; and the water returning unit is fixed outside each aquaculture tank and includes a pump and a water return tube.

The invention provides a microbial carrier. The microbial carrier is in a porous solid form and contains a biodegradation material with the weight content of 50% or more, the specific surface area is 100-900 m<2>/g, the average pore size is 20 nm-0.4 mm, the microbial carrier is at least of a two-layer structure, and the average pore size of the surface layer is 50 nm-0.4 mm. The microbial carrier can provide larger living environment for microorganisms, provide a carbon source for growth and propagation of microorganisms particularly denitrifying bacteria, improve the concentration of microorganisms in unit volume and improve the microbiological treatment efficiency.