9 results about "Rhizobia" patented technology

The utility model relates to the technical field of plowing devices, in particular to a plowing device for corn and soybean rotation cultivation, which comprises a shell, an extension assembly is arranged in the shell, the extension assembly comprises a first motor fixedly connected with the inner side wall of the shell, the output end of the first motor is fixedly connected with a linkage rod, and the linkage rod is fixedly connected with the inner side wall of the shell. A plurality of rotating discs are fixedly connected to the side wall of the linkage rod, and a plurality of fixing rails are fixed to the inner side wall of the shell. Through cooperative arrangement of a first motor, a rotating disc and an extension rod, adjustment of the soil digging depth of the soil loosening hoe is achieved, corn roots are relatively deep, a deep loosened soil layer is needed to take roots and absorb nutrients in the early growth stage, soybean roots are relatively shallow, and rhizobium activities exist, so that the soil digging depth of the soil loosening hoe is adjusted. A relatively shallow loose soil layer is needed to ensure the activity of the rhizobium and the normal growth of the root system, the soil digging depth is accurately adjusted, the most suitable soil condition is created for the two crops, the root system development is promoted, and the crop yield and quality are improved.

This invention discloses a nitrogen-potassium synergistic fertilization method suitable for alfalfa cultivation in saline-alkali land, belonging to the field of agricultural fertilization technology. Addressing the problem that existing technologies lack specific alfalfa fertilization methods for saline-alkali land and fail to stimulate nitrogen fixation by rhizobia through nutrient synergy, resulting in high nitrogen fertilizer dependence and low utilization, the invention includes: measuring the rhizosphere soil electrical conductivity before sowing or before the greening stage to classify the degree of saline-alkali stress, and measuring the nitrate nitrogen content; dynamically adjusting the mass ratio of nitrogen to potassium oxide in the fertilizer based on the nitrate nitrogen content and salinity level; selecting a baseline ratio based on the salinity level when soil nitrate nitrogen is low, halving nitrogen application when moderate, and stopping nitrogen fertilizer application when sufficient; applying exogenous nitrogen fertilizer twice during the greening to initial flowering stage, and applying potassium fertilizer containing potassium humate once during the greening to branching stage, with the potassium humate supplying no less than 30% of the total potassium. This method is mainly used for alfalfa cultivation in saline-alkali land, achieving efficient synergistic utilization of nitrogen and potassium and alleviating saline-alkali stress.

The invention provides a coal gangue rapid soil formation method and application thereof in ditch filling and land reclamation, and belongs to the technical field of coal gangue ecological utilization. The coal gangue rapid soil formation method comprises the following steps that a foundation layer, a parent material-like layer, a soil-like layer and an ecological surface layer are sequentially constructed in a gully from bottom to top, and ecological stockpiling is carried out; the base layer is formed by a flexible biological impermeable material and a compound bacteria solution A; the parent material-like layer is formed by low-sulfur coal gangue, an improved matrix A and a bacillus pasteurii liquid; the soil-like layer is formed by high-sulfur coal gangue, an improved matrix B and a compound bacteria solution A; the ecological surface layer is formed by an ecological base material, a compound bacteria solution B and plant seeds; the compound bacteria liquid A in the base layer and the soil-like layer comprises acidophilic sulfate reducing bacteria and bacillus pasteurii, and the compound bacteria liquid B comprises bacillus subtilis, rhizobium and nitrogen-fixing bacteria. By adopting the method disclosed by the invention, harmless and large-scale digestion treatment of the coal gangue can be realized.

The application discloses a nitrogen-potassium synergistic fertilization method suitable for alfalfa planting in saline-alkali soil and belongs to the technical field of agricultural fertilization. In view of the fact that alfalfa fertilization methods for saline-alkali soil are lacked in the prior art, the nodule bacteria nitrogen fixation cannot be excited by nutrient synergy, nitrogen fertilizer is highly dependent, and the utilization rate is low, the application scheme comprises the following steps: determining the rhizosphere soil conductivity before sowing or before the green stage to divide the saline-alkali stress degree, and determining the nitrate nitrogen content; according to the mass ratio of nitrogen to potassium oxide in the fertilizer is dynamically adjusted according to the nitrate nitrogen content and the saline-alkali degree, when the soil nitrate nitrogen is low, the standard ratio is selected according to the saline-alkali degree, when it is moderate, the nitrogen is halved, and when it is sufficient, the nitrogen fertilizer is stopped; the exogenous nitrogen fertilizer is applied twice from the green stage to the initial flowering stage, the potassium fertilizer containing potassium fulvate is once deep applied from the green stage to the branching stage, and the potassium supply amount of the potassium fulvate is not less than 30% of the total potassium amount. The method is mainly used for alfalfa planting in saline-alkali soil, realizes nitrogen-potassium synergistic high-efficiency utilization, and relieves the saline-alkali stress.

The invention relates to a microbial organic fertilizer and application thereof, and belongs to the technical field of biology, the microbial organic fertilizer comprises the following components: a component A comprising the following components in parts by mass: 4-6 parts of soybean peptide powder, 18-22 parts of soybean enzymatic hydrolysate, 18-24 parts of corncob furfural residue powder, 14-16 parts of mineral source humic acid, 12-18 parts of mushroom residue powder, 6-15 parts of rice hull powder, 6-9 parts of oyster shell powder, and 5-7 parts of cattle bone powder; the component B comprises a Japanese bradyrhizobium inoculant, a micrococcus luteus inoculant and a bacillus subtilis inoculant in a mass ratio of 1: (1-2): (3-6); and the mass of the component B is 1-3% of that of the component A. The bio-organic fertilizer provided by the invention can effectively prevent and treat clubroot caused by plasmodiophora brassicae and improve the yield of Chinese cabbages; the biological organic fertilizer provided by the invention can be used for effectively preventing and treating tomato bacterial wilt caused by pseudomonas solanacearum and increasing the yield of tomatoes; the bio-organic fertilizer provided by the invention can be used for effectively preventing and treating tea tree root rot caused by hard aschersonia, and the yield and the quality of tea leaves are improved.

The application relates to the technical field of genetic engineering, and particularly relates to application of a GmRDR3 gene in improving nodulation nitrogen fixation capacity and increasing yield GmRDR3 The application knocks out a GmRDR3 gene in soybeans by using a CRISPR / Cas9 technology RDR3 It is found through nodulation phenotype identification of the homozygous deletion mutant after inoculation of USDA110 rhizobium that the nodulation number and nitrogen content of the GmRDR3-KO mutant are obviously higher than those of the wild type, and the plant height of the soybean also obviously changes; after investigation of eight agronomic characters of stem diameter, plant height, effective branch, main stem node number, pod number per plant, grain number per plant and yield per plant of the GmRDR3-KO mutant and the WT after field planting, it is found that the stem diameter, plant height, effective branch, main stem node number, pod number per plant, grain number per plant and yield per plant of the GmRDR3-KO mutant are all obviously improved compared with the wild type, the mature period is delayed by 5-7 days, and the lodging resistance is enhanced.

The invention discloses bradyrhizobium guangdongense APHNZY-24-3 which has the functions of efficiently promoting growth, promoting nodulation and inhibiting aspergillus flavus and application of the bradyrhizobium guangdongense APHNZY-24-3, and the serial number of the bradyrhizobium guangdongense APHNZY-24-3 is CCTCC (China Center for Type Culture Collection) M 2026225. According to the invention, integration of two major functions, namely a characteristic antibacterial volatile matter for producing benzothiazole and efficient symbiotic nitrogen fixation, on a single strain is realized in the bradyrhizobium guangdongensis for the first time. The action mechanism is clear: the benzothiazole produced by the strain can accurately down-regulate the expression of a toxin synthesis key gene and a spore development core gene in aspergillus flavus. Through one-time inoculation, double targets of growth promotion and nitrogen fixation and source bacteriostasis can be synchronously realized in the rhizosphere-legume microdomain of the peanuts, and particularly aiming at the biological characteristic of underground fruiting of the peanuts, a biological fumigation protection layer is constructed in soil in the critical legume development period, and aspergillus flavus infection and toxin production are continuously blocked.

This invention relates to the field of ecological technology, specifically disclosing a soil matrix containing mud cake, its preparation method, and its application in ecological restoration. The soil matrix of this invention comprises a solid mixture and water. The solid mixture includes: 0.005%-0.01% nitrogen-fixing compound bacterial agent, 20%-45% organic compound slow-release material, 25%-40% mud cake, 28%-42% deep subsoil, 0.05%-0.1% cationic polyacrylamide, and 0.2%-0.4% vinyl acetate-ethylene carbonate copolymer; the nitrogen-fixing compound bacterial agent includes rhizobia, Azotobacter chrysophyllariae, Bacillus megaterium, and Azospirillum; the organic compound slow-release material includes organic fertilizer, wood flour, and fertilizer. This invention fully utilizes the large amount of mud cake generated in hydropower development and other projects, developing it into a substrate suitable for plant growth, especially providing a platform and carrier for plant growth on slopes, saving land resources, and possessing advantages such as high operability, low cost, and good ecological restoration effect.

The invention discloses an efficient composite intercropping method for pomegranate, dry rice and broad bean grain, which scientifically combines pomegranate (economic output), dry rice (grain output) and broad bean (partial economic output and biological nitrogen fixation and land cultivation), realizes functional complementation, and breaks through the single target limitation of the traditional intercropping mode. Through effective connection of crops for rotation of'broad bean sowing in winter and upland rice sowing in spring ', full utilization of light and heat resources and land in the orchard from winter to spring, summer and autumn in the next year is realized, and the multiple cropping index is remarkably improved; the pomegranate utilizes the upper-layer three-dimensional space, and the upland rice and the broad beans utilize the middle-lower-layer space, so that an efficient three-dimensional lighting structure and space combination are formed; the nitrogen fixation effect of the rhizobium of the broad beans can directly increase the nitrogen nutrition of the soil, the straw returning can increase the organic matters of the soil, and the built-in fertilizing mechanism of the orchard effectively relieves the consumption of the intercropping system on the soil fertility and ensures the sustainability of the mode.