177 results about "Rhizobia" patented technology

The invention relates to a microbial agent for purifying river wastewater and a preparation method of the microbial agent. The microbial agent comprises the following components in parts by weight: 20-40 parts of bacillus natto, 10-30 parts of nitrobacteria, 5-15 parts of white-rot fungi, 5-15 parts of pseudomonas and 10-40 parts of photosynthetic bacteria. The microbial agent is well-adapted and has the capabilities of fast forming dominant bacterial communities and replenishing indigenous rhizobia to the water body; and the microbial agent can be used for reducing the COD (Chemical Oxygen Demand), NH3-H, total nitrogen, TP (Total Phosphorus) and the like, removing odor and color and producing high-activity bio-enzyme to suppress the growth of harmful bacteria and acceleratively decompose residual baits as well as animal and plant residues, and a remarkable water quality purifying effect is achieved.

The invention relates to a compound microbial preparation for promoting paddy growth and a preparation method thereof. The compound microbial preparation for promoting paddy growth is characterized by consisting of 20-40 parts by weight of a microbial strain culture and 60-80 parts by weight of a microbial carrier; wherein, the microbial strain culture consists of the following raw materials in parts by weight: 2-8 parts of bacilli subtilis, 2-8 parts of bacilli megaterium, 5-10 parts of saccharomyces cerevisiae and 1-4 parts of rhizobia; the microbial carrier is zeolite or bentonite or mixture thereof. The compound microbial preparation has the advantages of promoting growth of beneficial microbes, enhancing absorption of nitrogen, phosphorous, potassium and other organic substances for paddy root system and promoting normal growth of the root system, thus promoting the paddy growth and reducing chemical fertilizer application.

The invention relates to a cultivation method of alfalfa. The cultivation method includes the steps: in the land selection step, a plot with deep, thick and loose soil and less prone to waterlogging is selected, the plot is tilled, crop rotation is performed, gramineous crops or root crops are planted as previous crops as required, and continuous cropping of leguminous crops as precious crops is forbidden; in the land preparation step, 28cm deep cultivation is performed after the previous crops are harvested, the surface is leveled in time, weeds are removed, raking, leveling and compacting are performed, and moisture preservation and water storage are performed; in the base fertilizer application step, organic fertilizer special for alfalfa is used, and 20kg-25kg of the organic fertilizer is applied per mu as base fertilizer; in the variety selection step, AiFeiNiTe alfalfa is selected; in the seed treatment step, seeds are cleaned and aired for 2-3 days before sowing and are then soaked in seed soaking agent and subjected to nodule bacteria inoculation.

The invention discloses a micro-ecological flora of crop root system probiotics and an application thereof. The micro-ecological flora comprises a leading flora, a balance flora, a basic flora, a secondary-function flora and a special-function flora, wherein the leading flora comprises 25-35 parts of nitrogen-fixing bacteria by mass; the balance flora comprises 17-23 parts of phosphate solubilizing bacteria and 17-23 parts of potassium solubilizing bacteria by mass; the basic flora comprises 2-7 parts of yeast, 2-7 parts of rhizobia and 2-7 parts of photosynthetic bacteria by mass; the secondary-function flora comprises 2-7 parts of bacillus and 2-7 parts of actinomycetes by mass; the special-function flora comprises 2-4 parts of white rot fungi and 1-3 parts of pseudomonas. In the micro-ecological flora disclosed by the invention, the strain stability is good, functional microorganisms can be effectively planted in soil, and the functions of improving the crop yield and quality, improving the soil, detoxifying heavy metals and resisting diseases and pests can be realized.

The invention relates to a kind of radiating legume bacteria that can reduce the content of nitrosamine in tobacco and its application. The radiating legume bacteria, Rhizobium radiobacter KenLXR34, of the invention has been kept by common microorganism center of China microorganism storing management committee, its number is CGMCC NO:0986. the main character of the invention follows this, screen out the radiating legume bacteria, KenLX34, which can reduce the content of nitrosamine in tobacco markedly, after fermenting culturing in liquid, it can be used for tobacco to reduce the content of TSNA in smoke. If dip footstalk in strain hanging liquid, the depression ratio is highest, it can reach 98.28%. so the invention has favourable and latent application.

The invention discloses a fertilizer for peanuts. The fertilizer is characterized by being prepared from 5-15 parts (in parts by weight) of microbial agent, 5-15 parts of pesticidal microbial agent, 0.5-1 part of zinc sulfate, 0.5-1 part of borax, 1-2 parts of magnesium sulfate, 5-15 parts of clay and 50-80 parts of chemical fertilizer rich in nitrogen, phosphor and potassium. The fertilizer for peanuts disclosed by the invention achieves effect of activating phosphor potassium and medium trace elements and effects of other biological bacteria, increases functions of the fertilizer, has pesticidal effect, and can promote formation of peanut rhizobium and improve fertilizer supply effect of the fertilizer.

The invention discloses an organic fertilizer containing medical dregs and biological germs and a preparation method thereof. The organic fertilizer containing medical dregs and biological germs, provided by the invention, comprises the following components in parts by weight: 15 parts of Chinese prickly meal, 10 parts of bean meal, 15 parts of plant ash, 20 parts of medicine residue, 15 parts of corm pulp, 10 parts of bone meal, 10 parts of bentonite and 5 parts of biological germ; and the biological germ is either any one of or the mixture of more than one of bacillus megatherium, rhizobium japonicum, basil rhizobia and EM flora. According to the organic fertilizer provided by the invention, the Chinese prickly meal and the medicine residue are added in the active ingredients, the Chinese prickly meal has an insecticidal property, and the medicine dregs has medical value, so that the synergistic effect of the two can reduce crop diseases and insect pests, reduce the usage of pesticide, and preserve the ecological environment. After using the organic fertilizer containing medical dregs and biological germs, the applicant found that the production of sweet potato increases more than 24 percent a year, the nutrient substance in the organic fertilizer is provided to the plants, the production cost is low, and the method can be widely used in agricultural field.

The invention discloses an organic fertilizer containing medical dregs and microelements and a preparation method thereof. The organic fertilizer containing medical dregs and microelements, provided by the invention, comprises the following components in parts by weight: 15 parts of Chinese prickly meal, 10 parts of bean meal, 15 parts of plant ash, 20 parts of medicine residue, 15 parts of corm pulp, 10 parts of bone meal, 10 parts of bentonite, 4.5 parts of biological germ and 0.5 part of micronutrients fertilizer; and the biological germ is either any one of or the mixture of more than one of bacillus megatherium, rhizobium japonicum, basil rhizobia and EM flora. According to the organic fertilizer provided by the invention, the Chinese prickly meal and the medicine residue are added in the active ingredients, the Chinese prickly meal has an insecticidal property, and the medicine dregs has medical value, so that the synergistic effect of the two can reduce crop diseases and insect pests, reduce the usage of pesticide, and preserve the ecological environment. After using the organic fertilizer containing medical dregs and microelements, the applicant found that the production of sweet potato increases more than 24 percent a year, the nutrient substance in the organic fertilizer is provided to the plants, the production cost is low, and the method can be widely used in agricultural field.

The invention relates to a composite microbial preparation for promoting the growth of rape and a preparation method thereof. The composite microbial preparation for promoting the growth of the rape is characterized by comprising 20-40 parts by weight of strain culturing substance and 60-80 parts by weight of microbial carrier, wherein the strain culturing substance comprises the following raw materials in parts by weight: 2-8 parts of bacillus subtilis, 2-8 parts of bacillus megaterium, 5-10 parts of rhodopseudomonas palustris and 1-4 parts of azotobacter chroococcum. The preparation method of the composite microbial preparation for promoting the growth of the rape comprises the following steps: culturing 2-8 parts of bacillus subtilis, 2-8 parts of bacillus megaterium and the rhodopseudomonas palustris together, culturing the azotobacter chroococcum independently, mixing and fermenting. The invention has the advantages of promoting the growth and the propagation of beneficial microorganisms, increasing the absorption of the root system of the rape to nitrogen, phosphorus, potassium and other organic substances and promoting the normal development of the root system, thus promoting the growth of the rape and reducing the applied chemical fertilizer.

The invention relates to a swine fermentation bed and a preparation method thereof. The swine fermentation bed contains a fermentation bed packing and a fermentation bed fungicide, wherein the fermentation bed packing per cubic meter is prepared from, by weight, rice husk 10-15 kg, sawdust 20-25 kg, vinasse 15-20 kg, straw 10-15 kg, rice bran 5-8 kg, corn meal 5-8 kg, rapeseed cake 4-6 kg, tea residues 3-5 kg, sediment 5-8 kg, wormcast 5-8 kg, wormwood 0.3-0.5 kg, Chinese fever vines 0.3-0.5 kg, garlic powder 0.1-0.3 kg and salt 0.5-0.8 kg. The fermentation bed fungicide is indigenous rhizobia, and the usage amount is 0.2-0.3 kg per cubic meter. The fermentation bed prepared by adopting the preparation method is reasonable in composition, straw is laid on the bottom layer of the fermentation bed, the thickness of the packing can be decreased, the temperature of the fermentation bed can be also kept, the Chinese herbal medicines are added to the composition, have the disinfection effect on swine excrement and meanwhile can promote proliferation of beneficial bacteria in swine intestinal tracts to promote growth, the environmental pollution brought by breeding is relieved by adopting the fermentation bed, and the purpose of zero breeding emissions is really achieved.

The invention discloses a bacterial strain of a root nodule nitrogen-fixing strain series RY3 and application thereof. The bacterial strain (Bradyrhizobium sp. RY3) is preserved in the China Center for Type Culture Collection on November 13th, 2009, and the collection number of the bacterial strain is CCTCC No: M 209267. The root nodule nitrogen-fixing strain series RY3 of the invention is grafted back to Stylosanthes guianensis TPRC2 and TPRC5 so that the fresh weight and dry weight of the Stylosanthes guianensis are increased by over triple. The RY3 is a highly-efficient bacterial strain which can ensure that the high yield of both the fresh weight and dry weight of the Stylosanthes guianensis can be achieved and the dissemination capacity of the Stylosanthes guianensis is stronger after the Stylosanthes guianensis is grafted with rhizobia; and after being released to the natural environment, the RY3 has no harm to human, animals and plants and does not pollute the environment.

The invention relates to an under-forest planting and ecological fertilizing method for konjak. According to the method, the konjak is planted by modes of the selection of forest lands, the planting of green manure and the reduction of the using amount of industrial fertilizers, so that the utilization efficiency of lands can be improved, and discarded areas in forests are changed into crop planting fields which can be utilized effectively; the konjak is shade-loving and can be prevented from being irradiated by sunlight under the forests while obtaining part of sunlight, so that the quality of the konjak can be improved, and the yield of a single plant can be increased; broad bean green manure is planted, so that a large number of ecological fertilizers can be provided, the using amount of the industrial fertilizers can be reduced, and the quality is improved while the yield is ensured; and the broad bean green manure contains a large number of symbiotic rhizobia, so a large number of ecological nitrogen fertilizers can be provided for the planting of the konjak, pathogenic bacteria are prevented from being generated on the konjak, and the diseases of the konjak are reduced.

The invention discloses a microbial fertilizer capable of improving soil structure, which is characterized by comprising the following components in parts by weight: 50-90 parts of livestock and poultry manure, 4-9 parts of sodium dihydrogen phosphate, 5-6 parts of disodium hydrogen phosphate, 6-15 parts of sodium polyphosphate, 4-9 parts of earthworm manure, 8-13 parts of rape cake, 40-55 parts of chicken manure, 15-35 parts of rice bran, 10-15 parts of wheat bran, 14-24 parts of bean straw, 5-9 parts of potato straw, 0.1-0.3 part of lactobacillus powder, 0.1-0.4 part of nitrobacteria powder and 0.1-0.2 part of rhizobia powder. The microbial fertilizer contains rich beneficial microbes, has the advantages of comprehensive and balanced nutrients and persistent effect, and can enhance the organic matter content in soil.

The invention discloses a method for increasing the alfalfa seed yield. The method comprises the steps that soil is prepared and fertilizers are applied from the middle ten days to the last ten days of March and membrane covering, bunch planting and alfalfa seed pelleting are carried out in the middle and last ten days of March; the seedlings of alfalfa emerge in the middle ten days of April, alfalfa enters the squaring stage in the last ten days of May, and 3-4g of mepiquat chloride is sprayed per mu at the squaring stage; boron fertilizers, potassium dihydrogen phosphate and molybdenum fertilizers are sprayed every other ten days from the bud stage to initial flowering, and watering is carried out once at every flowering stage in the middle and last ten days of June; pesticides are sprayed before flowering to control thrips and aphids and alfalfa enters the flowering stage in the first ten days of July; the seeds are harvested when 70-80% of seed pods become brown in the first ten days to the middle ten days of September. The method has the beneficial effects that the used coated seeds are inoculated with rhizobia, so that the application amount of nitrogen fertilizers is reduced, and environmental pollution of the nitrogen fertilizers is alleviated; the production cycle of the alfalfa seeds is shortened from two years to one year, namely, one season of seeds are additionally produced, so that the occupation ratio of the land on which alfalfa is planted in the current year can be reduced, the use ratio of the land is increased, and the seed yield and the incomes of growers are increased.

The invention relates, in part, to nucleic acid constructs, genetically modified host cells and methods employing such constructs and host cells to increase the production of 3-methyl-2-butenol from IPP. Thus, in some aspects, the invention provides a genetically modified host cell transformed with a nucleic acid construct encoding a fusion protein comprising a phosphatase capable of catalyzing the dephosphorylation of dimethylallyl diphosphate (DMAPP) linked to an IPP isomerase capable of converting IPP to DMAPP, wherein the nucleic acid construct is operably linked to a promoter. In some embodiments, the genetically modified host cell 5 further comprises a nucleic acid encoding a reductase that is capable of converting 3-methyl-2-butenol to 3-methyl-butanol. In some embodiments, the reductase is encoded by a nucleic acid construct introduced into the cell. In some embodiments, the IPP isomerase is a Type I isomerase. In some embodiments, the IPP isomerase is a Type II isomerase. In some embodiments, the host cell is selected from a group of taxonimcal classes consisting of 20 Escherichia, Enterobacter, Azotobacter, Erwinia, Bacillus, Pseudomonas, Klebsiella, Proteus, Salmonella, Serratia, Shigella, Rhizobia, Vitreoscilla, Synechococcus, Synechocystis, and Paracoccus taxonomical classes. In some embodiments, the host cell is an Escherichia coli cell. In some embodiments, the host cell is a fungal cell, such as a yeast cell. In some embodiments, the yeast cell is a Saccharomyces sp. cell. In some embodiments, the host cell is an algal, insect or mammalian cell line. In some embodiments, the phosphatase is nudB from E. coli. In some embodiments, the IPP isomerase is encoded by an idi gene from E. coli or idil gene from Saccharomyces cerevisiae.

The invention discloses special biological fertilizer for preventing and controlling peanut nematodosis and a preparation method thereof and belongs to the technical field of agricultural fertilizer. The fertilizer is prepared by mixing and evenly stirring a prepared compound microbial agent, a prepared insecticidal microbial agent and prepared inorganic fertilizer according to the adding percent. Due to the fact that the compound microbial agent and the insecticidal microbial agent are added to the fertilizer, the product can have the insecticidal action, promote formation of peanut nodule bacteria and improve the fertilizer supply effect, and is more suitable for peanuts. The fertilizer is definite in directivity and pertinence and very prominent in effect.

The invention discloses a mixed-sowing method of forage grass in a high altitude area of the Three Gorges reservoir area, comprising soil preparation before sowing, mixed sowing and fertilizer application, wherein the mixed sowing means mixed sowing of orchard grass seeds, trifolium repens seeds and alfalfa seeds, and specifically comprises the following steps of: performing row-spaced drilling on the orchard grass seeds and the alfalfa seeds, with row spacing of 0.3m; and uniformly sowing the trifolium repens seeds among the rows of the orchard grass seeds and the alfalfa seeds, wherein the dosage ratio of the orchard grass seeds to the trifolium repens seeds to the alfalfa seeds is 1: (1 to 1.6): (1 to 1.6), in parts by weight. The leguminous forage grass and gramineous forage grass selected for the mixed-sowing of forage grass disclosed by the invention are featured with heat resistance, drought resistance and leanness resistance, and are mixed-sowed with each other, thus adequately utilizing light energy and increasing photosynthates; additionally, the leguminous forage grass has a high protein content and the rhizobia of the leguminous forage grass has a function of nitrogen fixation, thus remarkably increasing the yield of the forage grass, and improving the quality of the forage grass and soil fertility, so that the advantages of the leguminous forage grass are very prominent. The method disclosed by the invention is simple and convenient, and simple in operation; and the artificial pasture established by the method has the characteristics of heat resistance, drought resistance and leanness resistance.

The invention discloses a special higher-utilization-ratio environment-friendly coated fertilizer for blueberries. The invention is characterized in that the fertilizer is prepared from the following raw materials in parts by weight: 1-3 parts of rhizobia, 1-2 parts of Azotobacter chroococcum, 1-3 parts of Bacillus megatherium, 34-37 parts of soybean meal, 30-34 parts of rape seed cake, 45-50 parts of clover stover, 1-2 parts of borax, 3-5 parts of bentonite, 7-11 parts of sodium humate, 4-6 parts of castor oil, 3-5 parts of diglycol, 2-5 parts of vitrified microbead, 14-20 parts of hydroxypropyl methyl cellulose, 4-7 parts of gypsum, 7-9 parts of effective microorganisms and 9-12 parts of soil conditioner. The fertilizer solves the problem of low utilization ratio of nitrogen; the nitrogen fixation fungus added in the fertilizer can reduce the application amount of the nitrogen fertilizer, and lowers the ecological environment for land pollution protection; proper proportions of phosphate fertilizer and potassium fertilizer are added according to the fertilizer demand characteristics of blueberries to satisfy the nutrients required in the whole blueberry growth period; the coating technique is utilized to enable the fertilizer to release nutrients slowly for a long time and implement balanced wants and needs of the blueberries, thereby avoiding the phenomenon of seedling burning due to overfertilization; and the fertilizer can promote blossoming and fruiting of the blueberries and enable the blueberries to come into the market in advance.

The invention discloses a method for interplanting camellia sinensis, alstonia scholaris and alfalfa. The method comprises the following steps: performing soil preparation and applying a base fertilizer; planting the camellia sinensis; planting the alstonia scholaris; inoculating the alfalfa with rhizobia; sowing the alfalfa; harvesting the alfalfa in real time; pruning the alstonia scholaris. According to the method, the alstonia scholaris is pruned in a natural open center shape with a single trunk, so that the light transmission rate of the alstonia scholaris can be effectively controlled, a certain shading effect can be provided for the camellia sinensis, and the growth of the alstonia scholaris is facilitated; the alfalfa is harvested in batches in earl time, so that the influence of the alfalfa on the camellia sinensis can be effectively reduced and the harvesting amount of the alfalfa can be increased; the camellia sinensis, the alstonia scholaris and the alfalfa are planted together, so that the soil resources can be fully utilized and the economic value can be increased.