49 results about "Rhizobia" patented technology

Disclosed herein are engineered rhizobia having nif clusters that enable the fixation of nitrogen under free-living conditions, as well as ammonium and oxygen tolerant nitrogen fixation under free-living conditions. Also provided are methods for producing nitrogen for consumption by a cereal crop using these engineered rhizobia.

Provided are a quality-improving and nitrogen-fixing coupled microbial agent and the use thereof. The quality-improving and nitrogen-fixing coupled microbial agent is a microbial composition, has coupled functions of improving quality and fixing nitrogen so as to improve the quality of products of leguminous crops, and has the functions of regulating and increasing the rhizobial abundance for the rhizosphere of leguminous crops and increasing the amount of nodules of leguminous crops. The microbial agent contains all gene sequences of nucleotide sequences shown as SEQ ID NO.1-12 or four or more gene sequences among same. When being applied to production of leguminous crops, such as soybeans, peanuts, peas, broad beans and cowpeas, the quality-improving and nitrogen-fixing coupled microbial agent has coupled functions of improving quality and fixing nitrogen and achieves quality improvement and nitrogen fixation and green yield increase. The microbial agent is easy to use, achieves remarkable social and ecological benefits, and facilitates popularization and use.

The present invention relates to the field of microorganisms and also to the field of crop fertilizers. Discloses is a use of an ARC microbial agent in the production of an ARC+ fertilizer. The ARC microbial agent is a microbial composition, has a coupled effect of toxicity control and nitrogen fixation and the effects of regulating and controlling the abundance of rhizobia in the rhizosphere of a leguminous crop and increasing the quantity of nodules in the leguminous crop, and contains DNA sequences as shown in SEQ ID Nos. 1-4. The fertilizer is a complex fertilizer / compound fertilizer, an organic fertilizer, or a microbial agent. Further disclosed are a method for producing an ARC+ fertilizer by compounding an ARC microbial agent with a fertilizer, an ARC+ fertilizer prepared by the method, and a use of the ARC+ fertilizer in crop production. The ARC+ fertilizer can act as both an ARC microbial agent and a fertilizer, and the operation step of mixing the two by a user can be removed, thereby avoiding the problem of non-uniform application of the ARC microbial agent.

The invention belongs to the technical field of greenhouse vegetable salinization soil improvement, and particularly relates to a preparation method of a compound microbial agent for greenhouse vegetable salinization soil improvement. Comprising the following steps: S1, preparing a fermentation substrate: mixing bran, molasses, ammonium sulfate, rice hull powder and water according to a mass ratio, and sterilizing at 121 DEG C for 30 minutes for later use; s2, preparing biochar: preparing modified biochar with MgO loading capacity of 4-6% and magnesium-loaded biochar with MgO loading capacity of 5-7%; s3, preparing a compound bacterium suspension: respectively preparing bacillus amyloliquefaciens, bacillus mucilaginosus, trichoderma viride, rhizobium and saccharomyces cerevisiae into 5 * 10 < 10 > CFU / mL bacterium suspensions, and mixing the bacterium suspensions according to a volume ratio to obtain the compound bacterium suspension. Through cooperation of multiple strains and charcoal-loaded magnesium, the salinization index of the facility vegetable soil can be remarkably reduced, the soil fertility is comprehensively improved, the micro-ecology of the soil is restored, and the growth and quality of vegetables are promoted.

The invention relates to the technical field of soil carbon improvement, and discloses a composite biological stimulant for tropical rainforest soil carbon improvement and a preparation method and application thereof, the composite biological stimulant is obtained by mixing a microbial preparation, an immobilized carrier, porous biochar, an inorganic mineral compound, a slow release fertilizer, a soil conditioner and a plant symbiotic inoculant; the microbial preparation comprises one or more of lactic acid bacteria, nitrifying bacteria, phosphate solubilizing bacteria, nitrogen-fixing bacteria and arbuscular mycorrhizal fungi; the inorganic mineral compound comprises feldspar, apatite and montmorillonite; the slow-release fertilizer comprises biochar, humic acid and phosphate; the soil conditioner comprises biological colloid prepared from seaweed extract and natural plant fiber prepared from straw and palm fiber; the plant symbiotic inoculant comprises rhizobium and / or endophyte. According to the composite biological stimulant, the soil carbon fixing capacity of the tropical rainforest is remarkably improved, the soil void structure is improved, and the plant growth capacity is enhanced.

The invention belongs to the technical field of microbial agents, and particularly relates to an Aspergillus heteromorphus AJF-Z1 strain and application thereof. The aspergillus heteromorpha is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 24, 2025, and the preservation number of the aspergillus heteromorpha is CGMCC No. 41922. The Aspergillus heteromorphus AJF-Z1 strain provided by the invention has an obvious promotion effect on soybean growth and nodulation condition improvement under a pot experiment condition, so that an efficient and stable technology for regulating and controlling synergistic nodulation of host soybean and rhizobium through exogenous fungi is constructed, and soybean root development, nodulation nitrogen fixation and stress resistance are regulated and controlled from multiple dimensions.

The present invention belongs to the technical field of mine tailings restoration, and specifically relates to a tailings soil conditioner, a mine tailings ecological restoration method and application. The tailings soil conditioner comprises raw materials in the following weight ratio: 100-120 parts of rice straw, 1-5 parts of rhizobia, and 30-35 parts of humic acid fertilizer; after applying the soil conditioner in the mine tailings area, leguminous plants are planted. The tailings soil conditioner can be used for mine tailings ecological restoration and reducing the metal content in the soil, and has good application prospects.

The present invention belongs to the field of microorganisms. Specifically, disclosed are an ARC microbial agent for quality improvement, nitrogen fixation and green yield increase, and the use thereof. The ARC microbial agent for quality improvement, nitrogen fixation and green yield increase has the effects of improving quality, fixing nitrogen and increasing yield, thereby improving the quality of a leguminous crop product. The ARC microbial agent has the effects of regulating and improving the abundance of rhizobia in the rhizosphere of the leguminous crop and increasing the number of nodules in the leguminous crop; and contains gene sequences as shown in SEQ ID NOs: 1-4. The ARC microbial agent can be used in the production of leguminous crops such as soybeans, peanuts, peas, broad beans and cowpeas, thereby realizing quality improvement, nitrogen fixation and green yield increase. In addition, the ARC microbial agent is simple to use, has significant social and ecological benefits, and is easy to popularize and apply.

The utility model relates to the technical field of plowing devices, in particular to a plowing device for corn and soybean rotation cultivation, which comprises a shell, an extension assembly is arranged in the shell, the extension assembly comprises a first motor fixedly connected with the inner side wall of the shell, the output end of the first motor is fixedly connected with a linkage rod, and the linkage rod is fixedly connected with the inner side wall of the shell. A plurality of rotating discs are fixedly connected to the side wall of the linkage rod, and a plurality of fixing rails are fixed to the inner side wall of the shell. Through cooperative arrangement of a first motor, a rotating disc and an extension rod, adjustment of the soil digging depth of the soil loosening hoe is achieved, corn roots are relatively deep, a deep loosened soil layer is needed to take roots and absorb nutrients in the early growth stage, soybean roots are relatively shallow, and rhizobium activities exist, so that the soil digging depth of the soil loosening hoe is adjusted. A relatively shallow loose soil layer is needed to ensure the activity of the rhizobium and the normal growth of the root system, the soil digging depth is accurately adjusted, the most suitable soil condition is created for the two crops, the root system development is promoted, and the crop yield and quality are improved.

The invention belongs to the technical field of soil remediation, and discloses a facility agriculture secondary salinization soil remediation and improvement method, which comprises the following steps: acquiring monitoring data of facility agriculture secondary salinization soil to be remedied and improved, and periodically acquiring soil salinity content conditions in different regions in different periods according to the monitoring data, the change rule of the salinity of the soil to be remediated is determined; the facility agriculture secondary salinization soil remediation modifier is prepared; according to the change rule of the salinity of the to-be-remedied soil, a soil remediation modifier is spread into the to-be-remedied and improved soil, and rotary tillage, watering and airing are conducted; an organic fertilizer and a bulking agent are applied to the soil to be remediated, and rotary tillage is conducted again; leguminous plant seeds and rhizobium are planted and irrigated with water, and the plants are fully returned to the field after growing up. The soil conditioner can effectively relieve soil hardening and reduce the content of salt and harmful microorganisms in soil, is good in effect and short in period, and effectively adjusts the pH value of the soil; by applying the organic fertilizer and the bulking agent, the looseness of the soil is ensured.

This invention discloses a nitrogen-potassium synergistic fertilization method suitable for alfalfa cultivation in saline-alkali land, belonging to the field of agricultural fertilization technology. Addressing the problem that existing technologies lack specific alfalfa fertilization methods for saline-alkali land and fail to stimulate nitrogen fixation by rhizobia through nutrient synergy, resulting in high nitrogen fertilizer dependence and low utilization, the invention includes: measuring the rhizosphere soil electrical conductivity before sowing or before the greening stage to classify the degree of saline-alkali stress, and measuring the nitrate nitrogen content; dynamically adjusting the mass ratio of nitrogen to potassium oxide in the fertilizer based on the nitrate nitrogen content and salinity level; selecting a baseline ratio based on the salinity level when soil nitrate nitrogen is low, halving nitrogen application when moderate, and stopping nitrogen fertilizer application when sufficient; applying exogenous nitrogen fertilizer twice during the greening to initial flowering stage, and applying potassium fertilizer containing potassium humate once during the greening to branching stage, with the potassium humate supplying no less than 30% of the total potassium. This method is mainly used for alfalfa cultivation in saline-alkali land, achieving efficient synergistic utilization of nitrogen and potassium and alleviating saline-alkali stress.

The invention discloses an acid-reducing and fertility-improving method for southern acidified red soil dry land and application of the acid-reducing and fertility-improving method. The acid-reducing and fertility-improving method can be divided into the following steps that 1, a high-water-solubility soil conditioner is broadcasted to increase the pH value of soil; step 2, applying a compound microbial fertilizer as a base fertilizer; 3, planting leguminous green manure; and 4, returning the leguminous green manure to the field. Aiming at the characteristic of low moisture content of dry land soil, the invention develops a high-water-solubility acid soil conditioner, and the soil pH and organic matter content can be quickly improved; the compound microbial fertilizer containing phosphate ore, potassium feldspar, dolomite and other materials capable of buffering soil acidification is developed, the soil acid buffer capacity is improved, and later soil re-acidification can be prevented; phosphorus-dissolving and potassium-dissolving microorganisms contained in the compound microbial fertilizer can continuously release nutrients in phosphate ore and potassium feldspar, the fertilizer efficiency is lasting, nodule bacteria can be symbiotic with leguminous green manure, the nitrogen fixation efficiency is improved, and the purposes of reducing acid and improving fertilizer are achieved.

The invention relates to the technical field of gene engineering, in particular to application of a soybean GmRDR3 gene in improvement of nodulation and nitrogen fixation capacity and increase of yield. An RDR3 gene in soybeans is knocked out by utilizing a CRISPR / Cas9 technology, nodulation phenotype identification after a homozygous deletion mutant is inoculated with USDA110 rhizobium shows that the nodulation number and nitrogen content of the GmRDR3-KO mutant are obviously higher than those of a wild type, and the plant height of the soybeans is also obviously changed; after the GmRDR3-KO mutant and WT are planted in a field, eight agronomic characters including stem diameter, plant height, effective branches, the number of main stem nodes, the number of single plant pods, the number of single plant grains and the yield of single plant are inspected, and the stem diameter, the plant height, the effective branches, the number of main stem nodes, the number of single plant pods, the number of single plant grains and the yield of single plant of the GmRDR3-KO mutant are obviously improved compared with those of a wild type, and the mature period is 5-7 days later; and the lodging-resistant capability is enhanced.

The invention provides a coal gangue rapid soil formation method and application thereof in ditch filling and land reclamation, and belongs to the technical field of coal gangue ecological utilization. The coal gangue rapid soil formation method comprises the following steps that a foundation layer, a parent material-like layer, a soil-like layer and an ecological surface layer are sequentially constructed in a gully from bottom to top, and ecological stockpiling is carried out; the base layer is formed by a flexible biological impermeable material and a compound bacteria solution A; the parent material-like layer is formed by low-sulfur coal gangue, an improved matrix A and a bacillus pasteurii liquid; the soil-like layer is formed by high-sulfur coal gangue, an improved matrix B and a compound bacteria solution A; the ecological surface layer is formed by an ecological base material, a compound bacteria solution B and plant seeds; the compound bacteria liquid A in the base layer and the soil-like layer comprises acidophilic sulfate reducing bacteria and bacillus pasteurii, and the compound bacteria liquid B comprises bacillus subtilis, rhizobium and nitrogen-fixing bacteria. By adopting the method disclosed by the invention, harmless and large-scale digestion treatment of the coal gangue can be realized.

Rhizobial infection is the key process for initiating symbiotic nitrogen-fixing root nodules in legumes, which requires specific recognition of signal molecules produced by the bacteria and their hosts. Here, it is established that rhizobial tRNA-derived small RNA fragments (tRFs) are crucial signal molecules modulating host nodulation, which uncovers a bacterial small RNA-mediated mechanism for prokaryote-eukaryote interaction. Transgenic plants are also provided that express a construct encoding rhizobial-derived tRNA, which is subsequently cleaved to produce artificial tRFs. Constructs and methods of producing the same are also provided, as well as modifications for repressing a mechanism for the negative regulation of nodulation present within plants.

The application discloses a nitrogen-potassium synergistic fertilization method suitable for alfalfa planting in saline-alkali soil and belongs to the technical field of agricultural fertilization. In view of the fact that alfalfa fertilization methods for saline-alkali soil are lacked in the prior art, the nodule bacteria nitrogen fixation cannot be excited by nutrient synergy, nitrogen fertilizer is highly dependent, and the utilization rate is low, the application scheme comprises the following steps: determining the rhizosphere soil conductivity before sowing or before the green stage to divide the saline-alkali stress degree, and determining the nitrate nitrogen content; according to the mass ratio of nitrogen to potassium oxide in the fertilizer is dynamically adjusted according to the nitrate nitrogen content and the saline-alkali degree, when the soil nitrate nitrogen is low, the standard ratio is selected according to the saline-alkali degree, when it is moderate, the nitrogen is halved, and when it is sufficient, the nitrogen fertilizer is stopped; the exogenous nitrogen fertilizer is applied twice from the green stage to the initial flowering stage, the potassium fertilizer containing potassium fulvate is once deep applied from the green stage to the branching stage, and the potassium supply amount of the potassium fulvate is not less than 30% of the total potassium amount. The method is mainly used for alfalfa planting in saline-alkali soil, realizes nitrogen-potassium synergistic high-efficiency utilization, and relieves the saline-alkali stress.

The invention belongs to the technical field of peanut cultivation methods, and particularly relates to a method for high-density shake-flask fermentation of bradyrhizobium anhuoshanense and application of the bradyrhizobium anhuoshanense. The improved YGA culture medium is used for carrying out shake flask fermentation on the peanut bradyrhizobium strain WYCCWR 15459, the high-density peanut bradyrhizobium inoculant is prepared, the effective viable count of the inoculant is larger than 10 < 11 > cfu / mL, after fermentation is carried out for 168 h, the inoculant shows significant difference from a YMA culture medium, an MYMA culture medium, a TY culture medium, a YMAR culture medium, a ZJC culture medium and a YDS culture medium, and finally OD600 is 1.26 times that of the YMA culture medium. By optimizing the formula and fermentation conditions of the YGA culture medium, high-density shake flask fermentation of the bradyrhizobium anhuoshanense microbial inoculum is achieved, and the preparation method is efficient, environmentally friendly, simple in technological process and relatively low in cost and has production and application potential.

The invention relates to a microbial organic fertilizer and application thereof, and belongs to the technical field of biology, the microbial organic fertilizer comprises the following components: a component A comprising the following components in parts by mass: 4-6 parts of soybean peptide powder, 18-22 parts of soybean enzymatic hydrolysate, 18-24 parts of corncob furfural residue powder, 14-16 parts of mineral source humic acid, 12-18 parts of mushroom residue powder, 6-15 parts of rice hull powder, 6-9 parts of oyster shell powder, and 5-7 parts of cattle bone powder; the component B comprises a Japanese bradyrhizobium inoculant, a micrococcus luteus inoculant and a bacillus subtilis inoculant in a mass ratio of 1: (1-2): (3-6); and the mass of the component B is 1-3% of that of the component A. The bio-organic fertilizer provided by the invention can effectively prevent and treat clubroot caused by plasmodiophora brassicae and improve the yield of Chinese cabbages; the biological organic fertilizer provided by the invention can be used for effectively preventing and treating tomato bacterial wilt caused by pseudomonas solanacearum and increasing the yield of tomatoes; the bio-organic fertilizer provided by the invention can be used for effectively preventing and treating tea tree root rot caused by hard aschersonia, and the yield and the quality of tea leaves are improved.

The invention discloses a soybean efficient nitrogen-fixing bradyrhizobium Bott59 and application thereof, and relates to the technical field of soybean planting, the key points of the technical scheme are that the efficient nitrogen-fixing bradyrhizobium is extracted from Ahonor black soil of Inner Mongolia, the strain can generate a good symbiotic relationship with soybeans, and the growth and development of soybean plants are remarkably promoted. The bradyrhizobium Bott59 can reduce the dependence of soybeans on nitrogen, effectively alleviates the problem of nitrogen balance, and is of great significance to development of green and sustainable agriculture and increase of soybean yield.

Provided are an ARC microbial agent for toxicity control, nitrogen fixation and yield increase, and a method for producing a crop by using same. The microbial agent is a microbial composition, has a coupled effect of toxicity control and nitrogen fixation, and has the effects of regulating and improving the abundance of rhizobia in the rhizosphere of a leguminous crop and increasing the number of nodules in the leguminous crop. The microbial agent contains DNA sequences as shown in SEQ ID NOs: 1-4.

The invention discloses a mixed microbial agent and application thereof in codonopsis pilosula planting. The composite flora is composed of phosphate solubilizing bacteria, phosphate solubilizing bacteria, phosphate solubilizing bacteria, phosphate solubilizing bacteria, phosphate solubilizing bacteria, phosphate solubilizing bacteria and phosphate solubilizing bacteria, potassium bacteria: pseudomonas aeruginosa and bacillus circulans; and nitrogen-fixing bacteria: autotrophic flavobacterium, clostridium pasteurianum and bradyrhizobium. Wherein the phosphate-solubilizing bacteria are wrapped by adopting a benzene diboronic acid-sodium alginate pH response material, the potassium-solubilizing bacteria are wrapped by adopting selenized sodium alginate-Ca < 2 + > salt ion response gel, and attapulgite is used as a carrier to form the slow-release bacterial agent. Phosphorus-solubilizing bacteria and potassium-solubilizing bacteria have a synergistic effect to maintain the optimal growth environment of codonopsis pilosula, trichoderma harzianum colonization is promoted through bacillus circulans exopolysaccharide to construct a synergistic network, and the acting effect of the fungicide is improved by combining dual-environment response release and boron-selenium element synergy. During application, the microbial inoculum and rotten stems and leaves of codonopsis pilosula are fermented to prepare a bacterial fertilizer for basal application, and then topdressing and flushing application are performed, so that the yield and polysaccharide content of codonopsis pilosula are remarkably increased.

The present invention belongs to the field of microorganisms. Disclosed is the use of an ARC microbial agent for controlling bacteria, reducing toxicity, improving quality and safety levels and reducing losses in the production of maize or wheat. The use of the ARC microbial agent for controlling bacteria, reducing toxicity, improving quality and safety levels and reducing losses in the production of maize or wheat. The ARC microbial agent is a microbial composition, which has a coupled effect of toxicity control and nitrogen fixation, has the effects of regulating and improving the abundance of rhizobia in the rhizosphere of a leguminous crop and increasing the number of nodules of the leguminous crop; and contains a DNA sequence as shown in SEQ ID NOs: 1-4. The ARC microbial agent is used for controlling bacteria, reducing toxicity, improving quality and safety levels and reducing losses in the production of maize or wheat, is simple to use, has significant social and ecological benefits, and is easy to popularize and apply.

The present invention belongs to the field of microorganisms. Disclosed is a use of an ARC microbial agent in the production of an ARC+soil conditioner. The ARC microbial agent is a microbial composition, has a coupled effect of toxin control and nitrogen fixation and the effects of improving the abundance of rhizobia in the rhizosphere of leguminous crops by means of regulation and increasing the nodule number of leguminous crops, and contains DNA sequences represented by SEQ ID Nos. 1-4. Further disclosed is a method for producing an ARC+soil conditioner by compounding an ARC microbial agent with a soil conditioner, an ARC+soil conditioner prepared by the method, and a use of the ARC+soil conditioner in crop production. The ARC+soil conditioner can exert both the function of an ARC microbial agent and the function of a soil conditioner, is simple and convenient to use, requires low cost, and achieves high benefits.

This invention discloses a highly efficient cultivation method for garlic, mushrooms, and soybeans in greenhouse vegetable fields, allowing for four harvests per year. Utilizing greenhouse vegetable fields, a crop of green garlic seedlings is intercropped with a crop of morel mushrooms, followed by a rotation of two crops of fresh soybeans. This allows for the harvesting of green garlic seedlings, morel mushrooms, and two crops of fresh soybeans within a year, increasing the multiple cropping index per unit area and achieving a highly efficient cultivation method with four harvests per year. The intercropping and rotation of garlic, mushrooms, and soybeans creates a virtuous cycle in the greenhouse vegetable field soil. The well-developed root system of green garlic seedlings can kill various harmful bacteria in the soil, facilitating the intercropping of morel mushrooms and producing garlic-flavored morel mushrooms. The abundant mycelium and mushroom stems remaining in the soil after the morel mushroom harvest, along with exogenous nutrient bags, can serve as natural organic fertilizer, increasing soil organic matter content and providing a rich nutrient source for the growth of fresh soybeans. The biological nitrogen fixation by the rhizobia in fresh soybeans can improve the soil, and the secreted acids can activate insoluble phosphorus in the soil, increasing soil fertility, reducing the need for fertilizers and pesticides, and promoting the healthy production of the next crop of organic green garlic seedlings.

This invention belongs to the field of anode material technology and discloses a biomass hard carbon anode material and its preparation method and application. The steps include: (1) culturing soybean rhizobium in a culture medium; (2) washing, drying, and pulverizing the soybean rhizobium to obtain soybean rhizobium powder; (3) pre-carbonizing the soybean rhizobium powder, followed by high-temperature carbonization and crushing; (4) acid leaching the product, and then obtaining a biomass hard carbon anode material after post-treatment. This invention, by using a biomass precursor to prepare biomass hard carbon material, can achieve uniform nitrogen doping, and further improve the electrochemical performance of the material by controlling the preparation process conditions, possessing broad market application prospects; at the same time, the preparation method of this invention is simple and suitable for large-scale industrial production.

The application relates to the technical field of genetic engineering, and particularly relates to application of a GmRDR3 gene in improving nodulation nitrogen fixation capacity and increasing yield GmRDR3 The application knocks out a GmRDR3 gene in soybeans by using a CRISPR / Cas9 technology RDR3 It is found through nodulation phenotype identification of the homozygous deletion mutant after inoculation of USDA110 rhizobium that the nodulation number and nitrogen content of the GmRDR3-KO mutant are obviously higher than those of the wild type, and the plant height of the soybean also obviously changes; after investigation of eight agronomic characters of stem diameter, plant height, effective branch, main stem node number, pod number per plant, grain number per plant and yield per plant of the GmRDR3-KO mutant and the WT after field planting, it is found that the stem diameter, plant height, effective branch, main stem node number, pod number per plant, grain number per plant and yield per plant of the GmRDR3-KO mutant are all obviously improved compared with the wild type, the mature period is delayed by 5-7 days, and the lodging resistance is enhanced.

A liquid composition the treatment of seeds of leguminous crops is disclosed. The liquid composition comprises phosphorus, molybdenum, cobalt and a solvent, wherein cobalt is present as vitamin B12 and the composition has a pH between 6.0 and 7.5, and wherein the composition comprises monoethanolamine. A method of preparing seeds of leguminous crops is also disclosed, comprising the steps of coating the seeds with the liquid composition, coating the seeds with a composition comprising a rhizobia inoculant, and drying the seeds coated with both compositions.

The invention discloses a soybean interplanting cultivation method for a high-altitude young kiwi fruit orchard. The soybean interplanting cultivation method comprises the following steps of land selection and preparation, good seed selection, sowing in a proper period, seed treatment, density adaptation, weed prevention and removal, reasonable fertilization, pest and disease damage prevention and control, timely harvesting, total straw returning to the field, kiwi fruit tree management and the like. The method aims at the early stage of kiwi fruit tree orchard building, low land utilization rate, fast weed growth, high management cost, only input and no output, fully utilizes the space ecological niche complementary advantage of soybeans and fruit trees, can reduce weed growth, increase multiple cropping index, realizes the purpose of growing with short length, fully utilizes the nitrogen fixation effect of soybean rhizobium, fertilizes soil, and improves the yield of kiwi fruit trees. Meanwhile, a new mode and a new path are explored for soybean planting expansion in northwest Yunnan province and even Yunnan province.

The invention discloses bradyrhizobium guangdongense APHNZY-24-3 which has the functions of efficiently promoting growth, promoting nodulation and inhibiting aspergillus flavus and application of the bradyrhizobium guangdongense APHNZY-24-3, and the serial number of the bradyrhizobium guangdongense APHNZY-24-3 is CCTCC (China Center for Type Culture Collection) M 2026225. According to the invention, integration of two major functions, namely a characteristic antibacterial volatile matter for producing benzothiazole and efficient symbiotic nitrogen fixation, on a single strain is realized in the bradyrhizobium guangdongensis for the first time. The action mechanism is clear: the benzothiazole produced by the strain can accurately down-regulate the expression of a toxin synthesis key gene and a spore development core gene in aspergillus flavus. Through one-time inoculation, double targets of growth promotion and nitrogen fixation and source bacteriostasis can be synchronously realized in the rhizosphere-legume microdomain of the peanuts, and particularly aiming at the biological characteristic of underground fruiting of the peanuts, a biological fumigation protection layer is constructed in soil in the critical legume development period, and aspergillus flavus infection and toxin production are continuously blocked.

The invention provides bradyrhizobium, a classification method thereof and application of the bradyrhizobium to improvement and compaction of soil. Specifically, the slow-growing rhizobium is used for strengthening the georgette composite planting system to improve and compact soil in a multi-dimensional mode, the slow-growing rhizobium is used for strengthening the phytoremediation technology, on one hand, the nitrogen fixation capacity of leguminous plants is enhanced, and on the other hand, the growth promoting effect on non-leguminous plants is achieved. The improvement method provided by the invention is suitable for street tree pits or other soil compaction sites, and meanwhile, the problem of serious compaction of the surface layer and the deep layer of the soil is solved.