18 results about "Rhizobia" patented technology

The utility model relates to the technical field of plowing devices, in particular to a plowing device for corn and soybean rotation cultivation, which comprises a shell, an extension assembly is arranged in the shell, the extension assembly comprises a first motor fixedly connected with the inner side wall of the shell, the output end of the first motor is fixedly connected with a linkage rod, and the linkage rod is fixedly connected with the inner side wall of the shell. A plurality of rotating discs are fixedly connected to the side wall of the linkage rod, and a plurality of fixing rails are fixed to the inner side wall of the shell. Through cooperative arrangement of a first motor, a rotating disc and an extension rod, adjustment of the soil digging depth of the soil loosening hoe is achieved, corn roots are relatively deep, a deep loosened soil layer is needed to take roots and absorb nutrients in the early growth stage, soybean roots are relatively shallow, and rhizobium activities exist, so that the soil digging depth of the soil loosening hoe is adjusted. A relatively shallow loose soil layer is needed to ensure the activity of the rhizobium and the normal growth of the root system, the soil digging depth is accurately adjusted, the most suitable soil condition is created for the two crops, the root system development is promoted, and the crop yield and quality are improved.

This invention discloses a nitrogen-potassium synergistic fertilization method suitable for alfalfa cultivation in saline-alkali land, belonging to the field of agricultural fertilization technology. Addressing the problem that existing technologies lack specific alfalfa fertilization methods for saline-alkali land and fail to stimulate nitrogen fixation by rhizobia through nutrient synergy, resulting in high nitrogen fertilizer dependence and low utilization, the invention includes: measuring the rhizosphere soil electrical conductivity before sowing or before the greening stage to classify the degree of saline-alkali stress, and measuring the nitrate nitrogen content; dynamically adjusting the mass ratio of nitrogen to potassium oxide in the fertilizer based on the nitrate nitrogen content and salinity level; selecting a baseline ratio based on the salinity level when soil nitrate nitrogen is low, halving nitrogen application when moderate, and stopping nitrogen fertilizer application when sufficient; applying exogenous nitrogen fertilizer twice during the greening to initial flowering stage, and applying potassium fertilizer containing potassium humate once during the greening to branching stage, with the potassium humate supplying no less than 30% of the total potassium. This method is mainly used for alfalfa cultivation in saline-alkali land, achieving efficient synergistic utilization of nitrogen and potassium and alleviating saline-alkali stress.

The invention relates to the technical field of gene engineering, in particular to application of a soybean GmRDR3 gene in improvement of nodulation and nitrogen fixation capacity and increase of yield. An RDR3 gene in soybeans is knocked out by utilizing a CRISPR / Cas9 technology, nodulation phenotype identification after a homozygous deletion mutant is inoculated with USDA110 rhizobium shows that the nodulation number and nitrogen content of the GmRDR3-KO mutant are obviously higher than those of a wild type, and the plant height of the soybeans is also obviously changed; after the GmRDR3-KO mutant and WT are planted in a field, eight agronomic characters including stem diameter, plant height, effective branches, the number of main stem nodes, the number of single plant pods, the number of single plant grains and the yield of single plant are inspected, and the stem diameter, the plant height, the effective branches, the number of main stem nodes, the number of single plant pods, the number of single plant grains and the yield of single plant of the GmRDR3-KO mutant are obviously improved compared with those of a wild type, and the mature period is 5-7 days later; and the lodging-resistant capability is enhanced.

The invention provides a coal gangue rapid soil formation method and application thereof in ditch filling and land reclamation, and belongs to the technical field of coal gangue ecological utilization. The coal gangue rapid soil formation method comprises the following steps that a foundation layer, a parent material-like layer, a soil-like layer and an ecological surface layer are sequentially constructed in a gully from bottom to top, and ecological stockpiling is carried out; the base layer is formed by a flexible biological impermeable material and a compound bacteria solution A; the parent material-like layer is formed by low-sulfur coal gangue, an improved matrix A and a bacillus pasteurii liquid; the soil-like layer is formed by high-sulfur coal gangue, an improved matrix B and a compound bacteria solution A; the ecological surface layer is formed by an ecological base material, a compound bacteria solution B and plant seeds; the compound bacteria liquid A in the base layer and the soil-like layer comprises acidophilic sulfate reducing bacteria and bacillus pasteurii, and the compound bacteria liquid B comprises bacillus subtilis, rhizobium and nitrogen-fixing bacteria. By adopting the method disclosed by the invention, harmless and large-scale digestion treatment of the coal gangue can be realized.

The application discloses a nitrogen-potassium synergistic fertilization method suitable for alfalfa planting in saline-alkali soil and belongs to the technical field of agricultural fertilization. In view of the fact that alfalfa fertilization methods for saline-alkali soil are lacked in the prior art, the nodule bacteria nitrogen fixation cannot be excited by nutrient synergy, nitrogen fertilizer is highly dependent, and the utilization rate is low, the application scheme comprises the following steps: determining the rhizosphere soil conductivity before sowing or before the green stage to divide the saline-alkali stress degree, and determining the nitrate nitrogen content; according to the mass ratio of nitrogen to potassium oxide in the fertilizer is dynamically adjusted according to the nitrate nitrogen content and the saline-alkali degree, when the soil nitrate nitrogen is low, the standard ratio is selected according to the saline-alkali degree, when it is moderate, the nitrogen is halved, and when it is sufficient, the nitrogen fertilizer is stopped; the exogenous nitrogen fertilizer is applied twice from the green stage to the initial flowering stage, the potassium fertilizer containing potassium fulvate is once deep applied from the green stage to the branching stage, and the potassium supply amount of the potassium fulvate is not less than 30% of the total potassium amount. The method is mainly used for alfalfa planting in saline-alkali soil, realizes nitrogen-potassium synergistic high-efficiency utilization, and relieves the saline-alkali stress.

The invention relates to a microbial organic fertilizer and application thereof, and belongs to the technical field of biology, the microbial organic fertilizer comprises the following components: a component A comprising the following components in parts by mass: 4-6 parts of soybean peptide powder, 18-22 parts of soybean enzymatic hydrolysate, 18-24 parts of corncob furfural residue powder, 14-16 parts of mineral source humic acid, 12-18 parts of mushroom residue powder, 6-15 parts of rice hull powder, 6-9 parts of oyster shell powder, and 5-7 parts of cattle bone powder; the component B comprises a Japanese bradyrhizobium inoculant, a micrococcus luteus inoculant and a bacillus subtilis inoculant in a mass ratio of 1: (1-2): (3-6); and the mass of the component B is 1-3% of that of the component A. The bio-organic fertilizer provided by the invention can effectively prevent and treat clubroot caused by plasmodiophora brassicae and improve the yield of Chinese cabbages; the biological organic fertilizer provided by the invention can be used for effectively preventing and treating tomato bacterial wilt caused by pseudomonas solanacearum and increasing the yield of tomatoes; the bio-organic fertilizer provided by the invention can be used for effectively preventing and treating tea tree root rot caused by hard aschersonia, and the yield and the quality of tea leaves are improved.

The invention discloses a mixed microbial agent and application thereof in codonopsis pilosula planting. The composite flora is composed of phosphate solubilizing bacteria, phosphate solubilizing bacteria, phosphate solubilizing bacteria, phosphate solubilizing bacteria, phosphate solubilizing bacteria, phosphate solubilizing bacteria and phosphate solubilizing bacteria, potassium bacteria: pseudomonas aeruginosa and bacillus circulans; and nitrogen-fixing bacteria: autotrophic flavobacterium, clostridium pasteurianum and bradyrhizobium. Wherein the phosphate-solubilizing bacteria are wrapped by adopting a benzene diboronic acid-sodium alginate pH response material, the potassium-solubilizing bacteria are wrapped by adopting selenized sodium alginate-Ca < 2 + > salt ion response gel, and attapulgite is used as a carrier to form the slow-release bacterial agent. Phosphorus-solubilizing bacteria and potassium-solubilizing bacteria have a synergistic effect to maintain the optimal growth environment of codonopsis pilosula, trichoderma harzianum colonization is promoted through bacillus circulans exopolysaccharide to construct a synergistic network, and the acting effect of the fungicide is improved by combining dual-environment response release and boron-selenium element synergy. During application, the microbial inoculum and rotten stems and leaves of codonopsis pilosula are fermented to prepare a bacterial fertilizer for basal application, and then topdressing and flushing application are performed, so that the yield and polysaccharide content of codonopsis pilosula are remarkably increased.

This invention discloses a highly efficient cultivation method for garlic, mushrooms, and soybeans in greenhouse vegetable fields, allowing for four harvests per year. Utilizing greenhouse vegetable fields, a crop of green garlic seedlings is intercropped with a crop of morel mushrooms, followed by a rotation of two crops of fresh soybeans. This allows for the harvesting of green garlic seedlings, morel mushrooms, and two crops of fresh soybeans within a year, increasing the multiple cropping index per unit area and achieving a highly efficient cultivation method with four harvests per year. The intercropping and rotation of garlic, mushrooms, and soybeans creates a virtuous cycle in the greenhouse vegetable field soil. The well-developed root system of green garlic seedlings can kill various harmful bacteria in the soil, facilitating the intercropping of morel mushrooms and producing garlic-flavored morel mushrooms. The abundant mycelium and mushroom stems remaining in the soil after the morel mushroom harvest, along with exogenous nutrient bags, can serve as natural organic fertilizer, increasing soil organic matter content and providing a rich nutrient source for the growth of fresh soybeans. The biological nitrogen fixation by the rhizobia in fresh soybeans can improve the soil, and the secreted acids can activate insoluble phosphorus in the soil, increasing soil fertility, reducing the need for fertilizers and pesticides, and promoting the healthy production of the next crop of organic green garlic seedlings.

This invention belongs to the field of anode material technology and discloses a biomass hard carbon anode material and its preparation method and application. The steps include: (1) culturing soybean rhizobium in a culture medium; (2) washing, drying, and pulverizing the soybean rhizobium to obtain soybean rhizobium powder; (3) pre-carbonizing the soybean rhizobium powder, followed by high-temperature carbonization and crushing; (4) acid leaching the product, and then obtaining a biomass hard carbon anode material after post-treatment. This invention, by using a biomass precursor to prepare biomass hard carbon material, can achieve uniform nitrogen doping, and further improve the electrochemical performance of the material by controlling the preparation process conditions, possessing broad market application prospects; at the same time, the preparation method of this invention is simple and suitable for large-scale industrial production.

The application relates to the technical field of genetic engineering, and particularly relates to application of a GmRDR3 gene in improving nodulation nitrogen fixation capacity and increasing yield GmRDR3 The application knocks out a GmRDR3 gene in soybeans by using a CRISPR / Cas9 technology RDR3 It is found through nodulation phenotype identification of the homozygous deletion mutant after inoculation of USDA110 rhizobium that the nodulation number and nitrogen content of the GmRDR3-KO mutant are obviously higher than those of the wild type, and the plant height of the soybean also obviously changes; after investigation of eight agronomic characters of stem diameter, plant height, effective branch, main stem node number, pod number per plant, grain number per plant and yield per plant of the GmRDR3-KO mutant and the WT after field planting, it is found that the stem diameter, plant height, effective branch, main stem node number, pod number per plant, grain number per plant and yield per plant of the GmRDR3-KO mutant are all obviously improved compared with the wild type, the mature period is delayed by 5-7 days, and the lodging resistance is enhanced.

The invention discloses bradyrhizobium guangdongense APHNZY-24-3 which has the functions of efficiently promoting growth, promoting nodulation and inhibiting aspergillus flavus and application of the bradyrhizobium guangdongense APHNZY-24-3, and the serial number of the bradyrhizobium guangdongense APHNZY-24-3 is CCTCC (China Center for Type Culture Collection) M 2026225. According to the invention, integration of two major functions, namely a characteristic antibacterial volatile matter for producing benzothiazole and efficient symbiotic nitrogen fixation, on a single strain is realized in the bradyrhizobium guangdongensis for the first time. The action mechanism is clear: the benzothiazole produced by the strain can accurately down-regulate the expression of a toxin synthesis key gene and a spore development core gene in aspergillus flavus. Through one-time inoculation, double targets of growth promotion and nitrogen fixation and source bacteriostasis can be synchronously realized in the rhizosphere-legume microdomain of the peanuts, and particularly aiming at the biological characteristic of underground fruiting of the peanuts, a biological fumigation protection layer is constructed in soil in the critical legume development period, and aspergillus flavus infection and toxin production are continuously blocked.

The invention provides bradyrhizobium, a classification method thereof and application of the bradyrhizobium to improvement and compaction of soil. Specifically, the slow-growing rhizobium is used for strengthening the georgette composite planting system to improve and compact soil in a multi-dimensional mode, the slow-growing rhizobium is used for strengthening the phytoremediation technology, on one hand, the nitrogen fixation capacity of leguminous plants is enhanced, and on the other hand, the growth promoting effect on non-leguminous plants is achieved. The improvement method provided by the invention is suitable for street tree pits or other soil compaction sites, and meanwhile, the problem of serious compaction of the surface layer and the deep layer of the soil is solved.

This invention relates to the field of ecological technology, specifically disclosing a soil matrix containing mud cake, its preparation method, and its application in ecological restoration. The soil matrix of this invention comprises a solid mixture and water. The solid mixture includes: 0.005%-0.01% nitrogen-fixing compound bacterial agent, 20%-45% organic compound slow-release material, 25%-40% mud cake, 28%-42% deep subsoil, 0.05%-0.1% cationic polyacrylamide, and 0.2%-0.4% vinyl acetate-ethylene carbonate copolymer; the nitrogen-fixing compound bacterial agent includes rhizobia, Azotobacter chrysophyllariae, Bacillus megaterium, and Azospirillum; the organic compound slow-release material includes organic fertilizer, wood flour, and fertilizer. This invention fully utilizes the large amount of mud cake generated in hydropower development and other projects, developing it into a substrate suitable for plant growth, especially providing a platform and carrier for plant growth on slopes, saving land resources, and possessing advantages such as high operability, low cost, and good ecological restoration effect.

The invention relates to a water-retaining material for growing plants, referred to as a water-retaining agent, comprising, by weight of dry matter, between 90 and 100% of at least one partially acetylated polysaccharide. The at least one polysaccharide comprises, by weight of dry matter, between 6 and 18% of acetyl groups. The water-retaining agent further comprises between 0 and 10% of one or more additives. The one or more additives comprise one or more fertilisers and / or one or more mycorrhizae and / or one or more rhizobia.

The invention discloses an efficient composite intercropping method for pomegranate, dry rice and broad bean grain, which scientifically combines pomegranate (economic output), dry rice (grain output) and broad bean (partial economic output and biological nitrogen fixation and land cultivation), realizes functional complementation, and breaks through the single target limitation of the traditional intercropping mode. Through effective connection of crops for rotation of'broad bean sowing in winter and upland rice sowing in spring ', full utilization of light and heat resources and land in the orchard from winter to spring, summer and autumn in the next year is realized, and the multiple cropping index is remarkably improved; the pomegranate utilizes the upper-layer three-dimensional space, and the upland rice and the broad beans utilize the middle-lower-layer space, so that an efficient three-dimensional lighting structure and space combination are formed; the nitrogen fixation effect of the rhizobium of the broad beans can directly increase the nitrogen nutrition of the soil, the straw returning can increase the organic matters of the soil, and the built-in fertilizing mechanism of the orchard effectively relieves the consumption of the intercropping system on the soil fertility and ensures the sustainability of the mode.

This invention discloses a planting method for promoting sugarcane to simultaneously tolerate aluminum and fix nitrogen, comprising the following steps: (1) planting site treatment; (2) pretreatment of sugarcane seed stalks; (3) planting; (4) seedling and tillering stage management; (5) elongation and maturity stage management; and (6) harvesting. The method of this invention involves applying wood ash and quicklime, which can partially alleviate soil aluminum toxicity; calcium magnesium phosphate fertilizer, charcoal powder, and rice husks are also added, effectively alleviating the toxicity of aluminum stress on sugarcane seedling roots and promoting nitrogen fixation by rhizobia; spraying nitrogen-fixing fertilizer on the roots of sugarcane seedlings and applying nitrogen-fixing fertilizer and aluminum-tolerant root fertilizer during the tillering stage can effectively alleviate the adverse effects of soil aluminum toxicity on sugarcane roots; further applying aluminum-tolerant root fertilizer at the early elongation stage, peak elongation stage, and maturity stage can not only effectively alleviate the adverse effects of soil aluminum toxicity on sugarcane roots but also effectively increase the nodulation rate of sugarcane roots.

The invention provides bradyrhizobium anhuiense GH-1 and application thereof, and relates to the technical field of microorganisms. Comprising peanut bradyrhizobium sp., the classification name of the peanut bradyrhizobium sp. Is Bradyrhizobium sp., the peanut bradyrhizobium sp. Is preserved in China General Microbiological Culture Collection Center on June 30, 2025, the preservation number of the peanut bradyrhizobium sp. Is CGMCC No.1.61832, and the preservation address of the peanut bradyrhizobium sp. Is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain belongs to a new strain of bradyrhizobium, can significantly promote nodulation and growth of peanuts and increase the yield of peanuts, and provides a new strain resource for improvement of nitrogen fixation efficiency in peanut production. In addition, the invention further provides a bacterial fertilizer capable of promoting peanut growth, the bacterial fertilizer is applied to field experiments, it is found that the bacterial fertilizer can remarkably improve the yield of peanut pods, and a new solution is provided for solving the problems of excessive use of nitrogen fertilizer and environmental pollution in peanut production.