24 results about "Soil nitrate" patented technology

The invention relates to the technical field of saline-alkali soil improvement, in particular to a compound conditioner for treating sulfate type saline-alkali soil and application of the compound conditioner. The compound conditioner is formed by compounding hydrotalcite, acidic zeolite and humic acid; wherein the hydrotalcite is nitrate intercalated MgAl-LDH or MgFe-LDH, and the molar ratio of divalent metal ions to trivalent metal ions of the hydrotalcite is (2: 1)-(4: 1); the acidic zeolite is clinoptilolite treated by a nitric acid solution; the humic acid is mineral source humic acid, and the purity of the humic acid is greater than 80%. The compound conditioner disclosed by the invention can mineralize Na < + > and SO4 < 2-> which are too high in content in saline-alkali soil, reduce the salt content and pH and prevent plants from osmotic stress and alkaline toxicity; the content of nitrate nitrogen and organic matters in soil is increased, and nutrient substances are provided for plants; the soil structure is improved, the water-retaining property and the air permeability are enhanced, the soil bulk density is reduced, and plant germination and root growth are facilitated.

The invention discloses a cow dung biochar composite material for saline-alkali soil improvement, and a preparation method and application thereof, and belongs to the technical field of cow dung material treatment. The method comprises the following steps: (1) air-drying cow dung, then carrying out oxygen-limited cracking, cooling and crushing to obtain cow dung original biochar; (2) mixing the cow dung original biochar with a calcium nitrate solution, and stirring for modification; and (3) after modification is finished, drying, carrying out oxygen-limited cracking, cooling, and crushing to obtain the cow dung biochar composite material. The calcium nitrate modified cow dung biochar composite material which can be popularized on a large scale and is low in cost is produced through the raw materials wide in source and the method easy to operate, and no pollutant is generated in the preparation process. The prepared composite material can reduce the pH value of saline-alkali soil, improve the physical and chemical properties of soil, has higher adsorption capacity on Na < + > in the soil, reduces the salt content of the soil, increases the nitrate nitrogen content of the soil, promotes plant growth, and meanwhile achieves the effect of recycling livestock waste.

The present application relates to the field of agricultural environmental protection method, particularly to a method for reducing the pollution of vegetable field soil non-point source and N2O.The method provided by the present application fully considers the carbon and nitrogen content, C / N and cellulose content of different exogenous carbon materials, reduces the application of chemical fertilizer nitrogen under the premise of ensuring the nitrogen supply of vegetables according to the nitrate nitrogen content of vegetable field soil, increases the amount of effective state carbon and the content of holocellulose by optimizing the combination and proportion of different exogenous carbon materials, improves the nitrate nitrogen assimilation of vegetable field soil, reduces the nitrogen leaching and soil N2O emission, and realizes the coordinated reduction and regulation of the pollution of vegetable field soil non-point source and N2O.The method is clear, simple, convenient, easy to implement.

The present invention discloses a method for effectively estimating the soil nitrate nitrogen assimilation rate, which comprises the following steps: (1) obtaining the physicochemical properties of organic matter; (2) obtaining the nitrate nitrogen assimilation rate of soil nitrate nitrogen; 15 Organic nitrogen using N tracer technology 15 The N recovery method calculates the soil nitrate nitrogen assimilation rate under the input of organic materials; (3) the data of step (1) and step (2) are input into SIMCA software, and the importance of the physical and chemical properties of organic materials to the soil nitrate nitrogen assimilation rate is evaluated using projection importance analysis to obtain a VIP diagram; (4) the physical and chemical properties of organic materials that best affect the soil nitrate nitrogen assimilation rate are determined based on the VIP diagram, and a regression equation is established. The present invention uses laboratory analysis and regression model establishment methods to quickly and effectively estimate the soil nitrate nitrogen assimilation rate, which can overcome the cumbersome field operations and technical deficiencies, and no longer requires the addition of organic materials in the subsequent evaluation of the soil nitrate nitrogen assimilation capacity under the input of organic materials. 15 N markers, save costs, and thus have good promotion and practical significance.

This invention discloses a cow dung biochar composite material for saline-alkali land improvement, its preparation method, and its uses, belonging to the field of cow dung material processing technology. It includes the following steps: (1) air-drying cow dung, then performing oxygen-limited pyrolysis, cooling, and pulverizing to obtain raw cow dung biochar; (2) mixing the raw cow dung biochar with calcium nitrate solution and then stirring for modification; (3) after modification, drying and performing oxygen-limited pyrolysis, cooling, and pulverizing to obtain the cow dung biochar composite material. This invention uses widely available raw materials and an easy-to-operate method to produce a calcium nitrate-modified cow dung biochar composite material that can be widely promoted and is inexpensive, and the preparation process generates no pollutants. The prepared composite material can lower the pH of saline-alkali land, improve the physical and chemical properties of the soil, and reduce the sodium content in the soil. + It has a stronger adsorption capacity, reduces soil salinity, increases soil nitrate nitrogen content, promotes plant growth, and achieves the effect of recycling livestock waste.

The invention discloses a low-toxicity method for measuring soil nitrate nitrogen 15N based on an optical cavity ring-down technology, which comprises the following steps: extracting 1 g of fresh soil with 10 ml of 2M KCl, determining the content of nitrate nitrogen in the soil through a flow analyzer, and diluting to 0.14-0.28 mgN / ml to obtain a soil extract for later use; the method comprises the following steps: adding 2ml of a soil leaching solution into a headspace bottle for later use, and then sequentially adding 0.2 ml of a 0.01 M VCl3 solution and 0.5 ml of 0.02 M hydroxylamine hydrochloride; transferring to a constant-temperature shaking table at the temperature of 37 DEG C, reacting for 16 hours at the speed of 125 rpm / min, extracting a gas sample by using a gas-tight injector, and injecting into an isotope measuring instrument based on an optical cavity ring-down technology to measure 15N of N2O as 15N of nitrate nitrogen. According to the method, the analysis efficiency of the whole experiment can be effectively improved, environmental hazards caused by use of high-toxicity chemical reagents are reduced, and meanwhile, the analysis cost is effectively reduced.

The invention relates to the technical field of agricultural resources and environment, in particular to a fertilization method for reducing nitrogen loss of an apple orchard. The apple branch biochar is prepared from the apple orchard pruning waste, and resource utilization of the waste is achieved. Through the synergistic effect of the apple branch charcoal, dicyandiamide and the reduced nitrogen fertilizer, the loss of nitrate nitrogen in soil is obviously slowed down, the conversion of ammonium nitrogen is inhibited, and a nitrogen cycle functional microflora is regulated, so that the application amount of the nitrogen fertilizer is reduced, the utilization rate of the nitrogen fertilizer is improved, and the environmental pollution is reduced; the growth of apple plants can be obviously promoted, the root configuration is improved, and the root activity and the nitrate reductase activity are improved.

The invention relates to the technical field of chemical component detection, and particularly discloses fluorescent test paper, a fluorescence analysis method and a fluorescence analysis device for visual online detection of soil nitrate nitrogen. According to the test paper, nitrate nitrogen is reduced into nitrite nitrogen by adopting zinc powder and a cadmium chloride solution, and then the nitrite nitrogen is dropwise added to fluorescent test paper prepared by soaking a mixed solution of para aminobenzoic acid, N-phenyl-1-naphthylamine and hydrochloric acid; under an acidic condition, nitrite nitrogen and para aminobenzoic acid generate diazonium salt, and then the diazonium salt reacts with N-phenyl-1-naphthylamine to form an azo compound, so that the original blue fluorescence on the test paper is gradually weakened along with the increase of the concentration of the nitrate nitrogen. According to the method, high-sensitivity and specific recognition of nitrate nitrogen is realized. And in combination with color identification and data analysis software of a smart phone, a portable and low-cost field detection system is constructed, and an efficient and practical new technical means is provided for visual quantitative detection of soil nitrate nitrogen.

This invention discloses a nitrogen-potassium synergistic fertilization method suitable for alfalfa cultivation in saline-alkali land, belonging to the field of agricultural fertilization technology. Addressing the problem that existing technologies lack specific alfalfa fertilization methods for saline-alkali land and fail to stimulate nitrogen fixation by rhizobia through nutrient synergy, resulting in high nitrogen fertilizer dependence and low utilization, the invention includes: measuring the rhizosphere soil electrical conductivity before sowing or before the greening stage to classify the degree of saline-alkali stress, and measuring the nitrate nitrogen content; dynamically adjusting the mass ratio of nitrogen to potassium oxide in the fertilizer based on the nitrate nitrogen content and salinity level; selecting a baseline ratio based on the salinity level when soil nitrate nitrogen is low, halving nitrogen application when moderate, and stopping nitrogen fertilizer application when sufficient; applying exogenous nitrogen fertilizer twice during the greening to initial flowering stage, and applying potassium fertilizer containing potassium humate once during the greening to branching stage, with the potassium humate supplying no less than 30% of the total potassium. This method is mainly used for alfalfa cultivation in saline-alkali land, achieving efficient synergistic utilization of nitrogen and potassium and alleviating saline-alkali stress.

The invention discloses a soil solution nitrate nitrogen dynamic prediction method based on sensor data flow, and belongs to the field of soil nitrate nitrogen prediction.The method comprises the following steps that samples are collected to construct original data, and the original data are processed to obtain processed data; and constructing a soil solution nitrate nitrogen prediction model based on the multivariable time sequence data, and inputting the processed data into the soil solution nitrate nitrogen prediction model to realize dynamic monitoring and prediction of the nitrate nitrogen concentration of the soil solution. According to the method, the sensor data flow is combined with the machine learning model, and an important breakthrough is made in the field of time sequence prediction. The combination not only reduces the cost of long-term positioning monitoring, but also improves the efficiency of data acquisition, so that the data excavation potential of agricultural basic long-term work is further exerted.

The present disclosure relates to a method for synergistically reducing non-point source pollution and nitrous oxide emission in vegetable field soil. The method provided by the present disclosure, by fully considering the carbon and nitrogen content, C / N ratio, and cellulose content of various exogenous carbon materials, reduce the application of nitrogen fertilizers based on the content of nitrate nitrogen in vegetable field soil while ensuring the nitrogen supply to vegetables, and increase the quantities of available carbon and the content of holocellulose by optimizing the combination and proportion of various exogenous carbon materials.

The application discloses a nitrogen-potassium synergistic fertilization method suitable for alfalfa planting in saline-alkali soil and belongs to the technical field of agricultural fertilization. In view of the fact that alfalfa fertilization methods for saline-alkali soil are lacked in the prior art, the nodule bacteria nitrogen fixation cannot be excited by nutrient synergy, nitrogen fertilizer is highly dependent, and the utilization rate is low, the application scheme comprises the following steps: determining the rhizosphere soil conductivity before sowing or before the green stage to divide the saline-alkali stress degree, and determining the nitrate nitrogen content; according to the mass ratio of nitrogen to potassium oxide in the fertilizer is dynamically adjusted according to the nitrate nitrogen content and the saline-alkali degree, when the soil nitrate nitrogen is low, the standard ratio is selected according to the saline-alkali degree, when it is moderate, the nitrogen is halved, and when it is sufficient, the nitrogen fertilizer is stopped; the exogenous nitrogen fertilizer is applied twice from the green stage to the initial flowering stage, the potassium fertilizer containing potassium fulvate is once deep applied from the green stage to the branching stage, and the potassium supply amount of the potassium fulvate is not less than 30% of the total potassium amount. The method is mainly used for alfalfa planting in saline-alkali soil, realizes nitrogen-potassium synergistic high-efficiency utilization, and relieves the saline-alkali stress.

The invention provides a vegetable ground surface source pollution emission reduction method based on a confinement space and application, and belongs to the technical field of agricultural environment. The confinement space comprises an interlayer and a plough layer; the interlayer is located below the plough layer; the interlayer comprises a high-carbon organic layer and a mineral material layer; the high-carbon organic layer is located below the mineral material layer; the high-carbon organic layer comprises a high-carbon organic material; the plough layer comprises plough layer soil and an organic carbon source material. An organic carbon source material and plough layer soil are mixed to serve as a plough layer, the assimilation capacity of the soil nitrate nitrogen can be improved, the soil nitrate nitrogen is assimilated and then stored, then the soil nitrate nitrogen is mineralized into crops to improve nutrients, the nutrients are intercepted in a confinement space through adsorption and interception of the mineral material layer and the high-carbon organic layer of the interlayer, the utilization of the nutrients of the root system is improved, and the yield is increased. The pollution risk of soil nitrogen and phosphorus leaching to surrounding water bodies is reduced in a layered and multi-way mode, and agricultural non-point source pollution caused by soil nitrate nitrogen accumulation and underground water fluctuation is reduced.

The invention relates to a microbial slow-release fertilizer, and discloses a microbial slow-release fertilizer and a preparation method thereof, the microbial slow-release fertilizer comprises 15-35 parts of activated DNRA bacteria immobilized beads, 10-15 parts of ammonium nitrate, 90-130 parts of urea, 5-15 parts of ammonium bicarbonate, 10-15 parts of ammonium sulfate, 10-15 parts of magnesium sulfate, 5-15 parts of borax, and 5-10 parts of activated carbon. According to the prepared microbial slow-release fertilizer, DNRA bacteria are embedded through the immobilized pellets, nitrate can be converted into ammonia nitrogen capable of being utilized by crops and soil microorganisms through the DNRA bacteria in soil, the fertilizer application cost can be reduced, pollution caused by the nitrate is reduced, and the yield of the fertilizer is increased. Meanwhile, the immobilized pellets are beneficial to maintaining relatively high biomass concentration and biological activity, and the nitrate nitrogen conversion stability of the DNRA bacteria in soil is enhanced.

The invention belongs to the technical field of agriculture, and particularly relates to an application of choline and an activity tracking separation method of choline in Xanthium sibiricum L. The method comprises the following steps: carrying out ethanol cold-soaking extraction on a whole plant of Xanthium sibiricum L., suspending the total extract obtained by concentrating an extracting solution with water, extracting with petroleum ether and ethyl acetate, and concentrating to obtain the choline. The method comprises the following steps: separating an aqueous phase extract obtained after the extraction liquid is concentrated by adopting adsorption resin to obtain an Fr.A part, separating the obtained Fr.A part by adopting sephadex, combining the obtained Fr.A-4 and Fr.A-5 into an Fr.AX part, separating by adopting sephadex, separating the obtained Fr.AX-5 part by adopting semi-preparative HPLC (High Performance Liquid Chromatography), and analyzing and identifying the obtained Fr.AX-5-1 part to determine the choline. The choline is obtained from the xanthium sibiricum through an activity tracking separation method, and it is found for the first time that the choline can significantly improve the net nitrification rate of soil of different habitats, so that the nitrification effect of the soil is promoted, and the content of nitrate nitrogen in the soil is improved.

The invention discloses a soil nitrate nitrogen assimilation and remineralization regulation and control method based on nitrogen dynamic adjustment. The method comprises the following steps: S1, soil pretreatment; s2, organic materials are added into the pretreated soil; s3, ammonium nitrogen continues to be added into the soil, and the concentration of the ammonium nitrogen in the soil is regulated and controlled; s4, cultivating the soil by controlling the humidity, the temperature and the period; s5, monitoring assimilation and remineralization rates of nitrate nitrogen in the soil; and S6, adjusting the addition amount of the organic material and the ammonium nitrogen according to the detection result of the step S5. According to the method, organic material input is combined with a modern testing technology and process means, a microbial regulation and control mechanism and key factors of assimilation and remineralization of nitrate nitrogen are defined, an organic material application scheme is optimized, and therefore the nitrogen conservation capacity of soil and the nitrogen utilization efficiency are improved.

The invention relates to a novel method for in-situ rapid extraction and detection of nitrate nitrogen in soil, and the principle is that a gradient diffusion film (DGT) technology can enrich and extract nitrate nitrogen in the soil in situ, and after elution, a fluorescent paper-based sensor is matched for color development reading, so that in-situ rapid extraction and detection of the nitrate nitrogen content in the soil are realized. The method mainly comprises the following steps: (1) preparing DGT for adsorbing nitrate nitrogen by using a developed nitrate nitrogen obligate adsorption material, placing the prepared DGT in a soil environment for a plurality of hours, taking out a binding membrane in a DGT device, and placing the binding membrane in an eluent for elution; and (2) carrying out simple pretreatment on the obtained eluent, carrying out fluorescent color development reaction by using a fluorescent paper-based sensor, and finally reading the content of nitrate nitrogen through color change. The invention provides a rapid, simple and convenient prediction method for evaluating the soil fertility and the absorption of the plants to the nitrogen in the soil.

The invention provides an inhibitor-containing water-soluble fertilizer special for greenhouse tomatoes as well as a preparation method and application of the inhibitor-containing water-soluble fertilizer, and belongs to the technical field of fertilizers. The special water-soluble fertilizer for greenhouse tomatoes comprises urea, monoammonium phosphate, potassium nitrate, boric acid, zinc sulfate, mineral source potassium fulvate, a growth regulator, amino acid, a nitrification inhibitor and a stable synergist. The invention further provides a preparation method and application of the special water-soluble fertilizer for the greenhouse tomatoes, and the special water-soluble fertilizer for the greenhouse tomatoes can promote root systems of the greenhouse tomatoes to grow downwards and enhance the photosynthesis capacity of leaves, so that the yield and quality of the tomatoes are improved; meanwhile, leaching loss of nitrate nitrogen in greenhouse tomato soil is reduced, and emission of greenhouse gas N2O and volatilization of NH3 are reduced.

The application discloses a kind of silkworm excrement carrier fixed pseudomonas aeruginosa PS1 biological agent and application thereof.Pseudomonas aeruginosa PS1 has strong degradation ability to PAEs, the degradation rate of DBP reaches 97.5%, the degradation rate of DEHP reaches 60.2%, and can use catechol, BA, PCA, PA, MBP, benzoic acid monobutyl ester, 4,5-dihydroxy phthalic acid, DEP, DMP and DOP and other various PAEs as substrate.The silkworm excrement is used as the carrier of fixed pseudomonas aeruginosa PS1, and the biological agent for repairing PAEs contaminated environment is prepared, is used to significantly improve soil pH, increase soil organic matter content and / or increase soil nitrate nitrogen and / or ammonium nitrogen content, increase the abundance of phylum Proteobacteria and / or phylum Synergistetes, and / or reduce the abundance of actinomycetales and / or Pseudonocardiales, so as to repair the environment contaminated by PAEs.

A real-time monitoring system for in situ soil nitrate concentration measurement comprising at least one 3D-printed and chemically functionalized potentiometric soil sensor for measuring nitrate levels and methods of making the real-time monitoring system. The real-time monitoring system is capable of providing multi-depth nitrate concentration measurements and continuous measurements over time.

The invention relates to the technical field of soil improvement, and discloses a porous complex microbial inoculant for promoting nitrate reduction to produce ammonium as well as a preparation method and application of the porous complex microbial inoculant. The invention relates to a porous complex microbial inoculant which is a modified porous mineral loaded with microorganisms with nitrate reducing capacity, the microorganism with the nitrate reducing capacity is prepared from at least one of Klebsiella pneumoniae, pseudomonas nitritiducen and Shewanella pneumoniae strain CN32, and the microorganism with the nitrate reducing capacity is prepared from at least one of Klebsiella pneumoniae, pseudomonas nitritiducen and Shewanella pneumoniae strain CN32. The porous complex microbial inoculant provided by the invention can promote the efficiency and rate of conversion from nitrate or nitrite to ammonium nitrogen, reduce the loss of nitrogen and the generation of greenhouse gases, has a compact structure and good stability, and can continuously play a role; the method is applied to the process of strengthening dissimilatory reduction of soil nitrate into ammonium, and soil nutrient loss and air pollution can be reduced.

The invention relates to the technical field of geological monitoring, in particular to a microorganism prediction method for monitoring, preventing and treating karst rocky desertification. Comprising the following steps: S1, respectively collecting land soil samples in a karst region and a non-karst region, and determining soil physicochemical properties including soil pH, soil total nitrogen, soil nitrate nitrogen, soil available phosphorus, soil total potassium, soil available potassium, soil exchangeable calcium, soil exchangeable sodium, soil chloride ion content and soil organic carbon; s2, the stony desertification succession process is analyzed through the vegetation coverage degree of the karst region, the correlation between the stony desertification succession process and the soil physicochemical properties is calculated, and the difference statistics of the soil physicochemical properties is obtained according to the correlation; s3, respectively extracting total DNA of the soil samples in each region, and analyzing to obtain composition characteristics of florae in the soil samples; s4, quantifying flora richness change through a specific measurement algorithm, and evaluating the stability of the microflora by adopting a parameter dispersion method; s5, taking bacteria and fungal species in the rocky desertification succession soil microbiome in the karst region and relative abundance in each corresponding sample as data, establishing an attribute feature set, and determining new biological indicator information based on an attribute data set; and S6, establishing a microorganism prediction model of karst rocky desertification monitoring control work.

The invention discloses an appropriate depth research method for complete profile distribution characteristics of nitrate nitrogen in loess plateau rain-fed apple orchard soil. The method comprises the following steps: (1) collecting literature data; (2) field acquisition of a soil profile; and (3) nitrate nitrogen indoor determination. In the east, south, west, north and middle areas of a loess plateau apple main producing area, through literature data arrangement, field soil sample collection and indoor analysis, the distribution characteristics of the nitrate nitrogen 6-m section of the soil of the apple orchard are disclosed, and whether the 6-m section is applicable or not is explored and the distribution characteristics of the nitrate nitrogen section of the soil of the loess plateau rain-fed apple orchard are completely disclosed.

The present application belongs to the field of agricultural production and environmental protection, and specifically relates to a root zone soil water and nitrogen growth and decline type drip irrigation method and a drip irrigation device. In the drip irrigation process, the water input amount of the left root zone soil of crops relative to the right root zone soil is reduced, and the nitrogen nutrient input amount of the left root zone soil of crops relative to the right root zone soil is increased, so that the water and nitrogen of the two sides of the root zone soil are input in a growth and decline type, the distribution of the water and nitrate nitrogen of the root zone soil on the two sides is regulated in a growth and decline type, and the leaching of soil nitrate nitrogen is reduced without negatively affecting the yield of crops.