Novel method for measuring activity of soil nitrate reductase
A technology of activity measurement and reductase, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, specific-purpose bioreactor/fermenter, etc. Different degrees, lower efficiency and other problems, to achieve the effect of improving accuracy and measurement efficiency, accurate and reliable measurement results, and improving measurement efficiency
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Embodiment 1
[0074] The new method of soil nitrate reductase activity assay that the embodiment of the present invention 1 provides compares with prior art, comprises:
[0075] Soil preparation: The soil samples in this example were collected from the 0-20 cm soil layer at the experimental station of the Agricultural College of Shihezi University, and the impurities were removed and passed through a 1 mm sieve. The soil samples were divided into two parts, one part was air-dried naturally (7-10 days), and the other part was dried at 180°C for 3 hours to inactivate the soil enzymes.
[0076] Preparation of reagents required for soil culture: 1% KNO 3 Solution (weigh 1.00g KNO 3 Dilute to 100mL with pure water), 1% glucose solution (weigh 1.00g glucose and dilute to 100mL with pure water), CaCO 3 (white powder), saturated solution of aluminum potassium sulfate (dissolve aluminum potassium sulfate in 100mL pure water to supersaturation, that is, ensure that crystals are precipitated in the ...
Embodiment 2
[0087] Embodiment 2 The new method of soil nitrate reductase activity assay that the embodiment of the present invention provides comprises:
[0088] Soil preparation: Collect soil samples in the 0-20cm soil layer, remove impurities and pass through a 1mm sieve. Each sample was divided into two parts, one part was air-dried naturally (7-10 days), and the other part was dried at 180°C for 3 hours to inactivate the soil enzymes.
[0089] Preparation of reagents required for soil culture: 1% KNO 3 Solution (weigh 1.00g KNO 3 Dilute to 100mL with pure water), 1% glucose solution (weigh 1.00g glucose and dilute to 100mL with pure water), CaCO 3 (white powder), saturated solution of aluminum potassium sulfate (dissolve aluminum potassium sulfate in 100mL pure water to supersaturation, that is, ensure that crystals are precipitated in the solution).
[0090] Reagent preparation on continuous flow analyzer: 0.1% CuSO 4 stock solution (0.10g CuSO 4 Dilute to 100mL with pure water)...
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