38 results about "Reductase activity" patented technology

A material includes a surface and a reactive agent that is located at the surface of the material, covalently attached to a backbone of the material, and/or located within the material. The reactive agent has nitrite reductase activity, nitrate reductase activity, and/or nitrosothiol reductase activity. The reactive agent also converts at least one of nitrites, nitrates and nitrosothiols to nitric oxide when in contact with blood. A reproducible nitrosothiol sensor is also disclosed.

The invention discloses a protein coded by a medicago sativa L. anthocyanin reductase gene, wherein the protein has an amino acid sequence shown in SEQ ID No:2 or an amino acid sequence which is obtained through substituting, deleting or adding one or a plurality of amino acid residues in the amino acid sequence shown in SEQ ID No:2 and still has anthocyanin reductase activity. The invention further relates to a coded gene of the protein, an expression vector and a cell which contain the gene, a method for culturing medicago sativa L. with high tannin content and application of the coded gene of the protein, the expression vector and the cell. Through the expression of the medicago sativa L. anthocyanin reductase gene (MsBAN) provided by the invention in the medicago sativa L., the tannin content of the medicago sativa L. can be obviously increased. The discovery of the gene provides a gene resource for overcoming the problem that main grass plants (in particular leguminous plants, such as the medicago sativa L.) easily cause livestock to suffer from tympanites, and plays an important role in the research of genetic engineering improved grasses.

Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.

The invention discloses long-chain non-coding RNA T5120 derived from arabidopsis thaliana and uses thereof. The nucleotide sequence of the long-chain non-coding RNA is shown as SEQ ID NO.1. The expression of the long-chain non-coding RNA is strongly induced by nitrate nitrogen. Compared with the recipient arabidopsis thaliana, the arabidopsis thaliana with the long-chain non-coding RNA transfectedhas higher nitrate nitrogen response capability and decreased nitrate nitrogen accumulation in the body, the nitrate nitrogen reductase activity and amino acid content are significantly increased, and the expression of nitrate nitrogen assimilation-related genes is significantly increased. T5120 acts on the downstream of NLP7 in the nitrate nitrogen signal regulation pathway.

Method of treating diseases associated with peroxisome proliferator-activated receptors by administering to a subject in need thereof an effective amount of 15-ketoprostaglandin-delta ¹³ - Reductase modulators. Also disclosed is a method of identifying a compound that inhibits the reductase activity, and a method of lowering blood glucose levels by administering to a subject an effective amount of the reductase inhibitor.

The invention discloses a nitrate nitrogen regulating and control gene ZmNRG2.7 from corn and a purpose thereof. The nucleotide sequence of the gene is shown as SEQ ID NO.1; the coded amino acid sequence is shown as SEQ ID NO.2. The expression of the gene is strongly induced by the nitrate nitrogen. Compared with receptor Arabidopsis, Arabidopsis transferred by the gene has the advantages that thenitrate nitrogen response capability is enhanced; the internal nitrate nitrogen accumulated quantity, the nitrate nitrogen reductase activity, the amino acid content and the full nitrogen content areobviously improved; the nitrogen element utilization rate of plants can be obviously improved.

The invention discloses a xanthone compound (1) capable of inhibiting 5 alpha-reductase. The xanthone compound is extracted from Swertia atroviolacea, the molecular formula is C18H18O5, the compound is named as: 6-(2-hydroxyethyl) -1,7-dimethoxy-3-methyl-xanthone, the xanthone compound has the structural formula which is defined in the description. The preparation method of the xanthone compound comprises the following steps: using the Swertia atroviolacea as the raw material, subjecting the raw material to extracting, MCI decoloration, silica gel column chromatography and high-pressure liquid chromatography to obtain the xanthone compound. The 5 alpha-reductase activity test on the xanthone compound shows that the xanthone compound has relatively good inhibition effect on the 5 alpha-reductase and the compound can be used as a lead compound for preparing a 5 alpha-reductase inhibitor.

The invention relates to a color development method for detecting the soil hydroxylamine reductase activity, in particular to a method that the extracting solution after the soil culture is added in with the ferric iron salt solution and the Alfa-Alfa'bipyridyl solution and has the color developed in the subacidity environment, and then the hydroxylamine concentration of the extracting solution are colorimetric-determined by a spectrophotometric method. The hydroxylamine concentration of the culture solution is acquired by comparing the extinction value of the extracting solution with the standard curve; as a result, the soil hydroxylamine reductase activity is worked out. The color reaction is carried out at the normal temperature, the acquired solution shows orange, and the color comparison wave length is 520nm. If the color comparison can not be completed in 40 minutes, the masking agent should be added in order to prevent the side reaction. Compared with the traditional color development method, the improved color development method has the advantages of high sensitivity, small system error, high accuracy and precision and little use level of the color developing agent, more accurate and reliable analysis result and better analysis reproducibility.

The present invention discloses a xanthomonadins synthesis related gene XC_4092. A gene sequence is as shown in SEQ ID NO.1. An encoded product has a 3-ketoacyl-ACP reductase activity and can catalyzea reduction reaction in xanthomonadins synthesis. The present invention further discloses an application of the gene XC_4092 in production of the colorless xanthan gum, xanthomonas campestris pathogenic strains of wild strains Xcc8004 are used as starting strains, the gene XC_4092 in a Xcc8004 genome is inactivated to complete gene modification to construct the colorless xanthan gum genetically engineered bacteria. Compared with the Xcc8004, the genetically engineered bacteria cannot synthesize xanthomonadins during fermentation processes, effectively improve appearance color of xanthan gum,and thus greatly improve quality of the xanthan gum product.

The present invention relates to a host cell having an elevated expression or activity of an enzyme as compared with the parent cell from which it has been derived, said enzyme having lactoyl-CoA reductase activity. Furthermore, provided is a method of producing lactaldehyde and/or 1,2-propanediol, said method comprising culturing said host cell and/or utilizing said enzyme to produce said compound.

Enzymes that reduce specific metal or metalloid ions to insoluble form are important to science. Peptides isolated from yeast- and phage-display libraries can affect the size and morphology of inorganic materials during their synthesis. Herein, an Se binding peptide was fused to an enzyme capable of reducing selenite (SeO3²⁻) to a Se⁰ nanoparticle (SeNP). The fusion of the Se binding peptide to the metalloid reductase provided size control of the resulting SeNP. The SeNP product also remains associated to the enzyme fusion. The Se binding peptide fusion to the enzyme increases the enzyme's SeO3²⁻ reductase activity. Modification of enzyme activity was absent, and the size control of particles was diminished when the Se binding peptide was added exogenously to the reaction mixture. Binding of the peptide is attributed to His based ligation that results in a conformational change to the peptide.

The present invention discloses a drug activator carrier comprising: a) particles having a metallic or metallic oxide core prepared from a paramagnetic material, said metallic core being coated with a coating material selected from polymer, metal or metal oxide; b) a biological material, having reductase activity, bound onto the metal coating the particles of step a), and wherein said biological material is capable of activating non-toxic pro-drugs into active and toxic drugs suitable for treating a disease; said drug activator carrier allowing targeted delivery of the toxic drug.

The invention provides a method for detecting dihyaropteridine reductase activity, a detection kit and application thereof. According to the method for detecting dihyaropteridine reductase activity, provided by the invention, the pretreatment step is simple, the sample usage amount is reduced, a 5mm blood stain is changed into two 3mm blood stains, and the actual clinical application is promoted; due to advanced split charging and preheating of the buffer solution, the detection time of each sample is shortened to 10min, and the detection throughput is greatly improved; due to the improved sample pretreatment in the method provided by the invention, the method is stable, the enzymatic activity curve is smooth, the RSD (Relative Standard Deviation) of within-run precision of quantitative results is less than or equal to 3.37%, the RSD of between-run precision is less than or equal to 7.82%, the reproducibility and accuracy of quantification are greatly improved, the detection time is short, the detection throughput is improved, and the detection requirements on clinical actual samples are met.