38 results about "Reductase activity" patented technology

A material includes a surface and a reactive agent that is located at the surface of the material, covalently attached to a backbone of the material, and / or located within the material. The reactive agent has nitrite reductase activity, nitrate reductase activity, and / or nitrosothiol reductase activity. The reactive agent also converts at least one of nitrites, nitrates and nitrosothiols to nitric oxide when in contact with blood. A reproducible nitrosothiol sensor is also disclosed.

The present invention relates to a composition for promoting hair growth. More particularly, the present invention comprises a component for inhibiting 5α-reductase activity, a functional component of cell activity, and an expansion component of peripheral blood vessels. A composition for promoting hair growth of the present invention has a superior effect on hair growth.

The invention discloses a protein coded by a medicago sativa L. anthocyanin reductase gene, wherein the protein has an amino acid sequence shown in SEQ ID No:2 or an amino acid sequence which is obtained through substituting, deleting or adding one or a plurality of amino acid residues in the amino acid sequence shown in SEQ ID No:2 and still has anthocyanin reductase activity. The invention further relates to a coded gene of the protein, an expression vector and a cell which contain the gene, a method for culturing medicago sativa L. with high tannin content and application of the coded gene of the protein, the expression vector and the cell. Through the expression of the medicago sativa L. anthocyanin reductase gene (MsBAN) provided by the invention in the medicago sativa L., the tannin content of the medicago sativa L. can be obviously increased. The discovery of the gene provides a gene resource for overcoming the problem that main grass plants (in particular leguminous plants, such as the medicago sativa L.) easily cause livestock to suffer from tympanites, and plays an important role in the research of genetic engineering improved grasses.

Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.

A novel carbonyl reductase useful for producing alcohol, particularly derivatives of (S)-4-halo-3-hydroxybutyrate ester, is provided. A novel carbonyl reductase derived from Kluyveromyces aestuarii and the nucleic acid encoding the enzyme are provided. The carbonyl reductase has excellent reductase activity and stereoselectivity. The carbonyl reductase reduces ketone to produce alcohol. It can be particularly advantageous when used in industrial production of (S)-4-halo-3-hydroxybutyrate ester.

The invention relates to the field of molecular biology, in particular to Cynoglossus semilaevis thioredoxin and a preparation method and application thereof. The thioredoxin is shown as an amino acid sequence in a sequence table SEQ ID No.1. The preparation method comprises the following steps of: constructing a plasmid pETrx, transforming the plasmid pETrx by using BL21(DE3) to obtain BL21(DE3) / pETrx, inducing by using isopropyl-beta-D-isopropylthiogalactoside, and purifying recombinant protein by using an affinity chromatography column to obtain the thioredoxin. The thioredoxin has remarkable reductase activity, a remarkable antioxidation effect and a remarkable cell growth promotion effect.

The invention discloses long-chain non-coding RNA T5120 derived from arabidopsis thaliana and uses thereof. The nucleotide sequence of the long-chain non-coding RNA is shown as SEQ ID NO.1. The expression of the long-chain non-coding RNA is strongly induced by nitrate nitrogen. Compared with the recipient arabidopsis thaliana, the arabidopsis thaliana with the long-chain non-coding RNA transfectedhas higher nitrate nitrogen response capability and decreased nitrate nitrogen accumulation in the body, the nitrate nitrogen reductase activity and amino acid content are significantly increased, and the expression of nitrate nitrogen assimilation-related genes is significantly increased. T5120 acts on the downstream of NLP7 in the nitrate nitrogen signal regulation pathway.

Method of treating diseases associated with peroxisome proliferator-activated receptors by administering to a subject in need thereof an effective amount of 15-ketoprostaglandin-delta 13 - Reductase modulators. Also disclosed is a method of identifying a compound that inhibits the reductase activity, and a method of lowering blood glucose levels by administering to a subject an effective amount of the reductase inhibitor.

The invention discloses a nitrate nitrogen regulating and control gene ZmNRG2.7 from corn and a purpose thereof. The nucleotide sequence of the gene is shown as SEQ ID NO.1; the coded amino acid sequence is shown as SEQ ID NO.2. The expression of the gene is strongly induced by the nitrate nitrogen. Compared with receptor Arabidopsis, Arabidopsis transferred by the gene has the advantages that thenitrate nitrogen response capability is enhanced; the internal nitrate nitrogen accumulated quantity, the nitrate nitrogen reductase activity, the amino acid content and the full nitrogen content areobviously improved; the nitrogen element utilization rate of plants can be obviously improved.

The present invention provides a process for production of optically pure quinuclidinol of formula-(I) by reduction of quinuclidinone of formula-(II) in presence of suitable oxidoreductase enzyme derived from Saccharomyces species. Formula (II, I) Moreover, the present enzyme works in presence of cofactor NADP where the cofactor is regenerated by suitable system. The present invention also provides a recombinant vector containing genes co expressing suitable polypeptides having oxido-reductase activity and polypeptide having capacity to regenerate the co-factor. The said vector is transformed in suitable host cell.

The invention discloses a xanthone compound (1) capable of inhibiting 5 alpha-reductase. The xanthone compound is extracted from Swertia atroviolacea, the molecular formula is C18H18O5, the compound is named as: 6-(2-hydroxyethyl) -1,7-dimethoxy-3-methyl-xanthone, the xanthone compound has the structural formula which is defined in the description. The preparation method of the xanthone compound comprises the following steps: using the Swertia atroviolacea as the raw material, subjecting the raw material to extracting, MCI decoloration, silica gel column chromatography and high-pressure liquid chromatography to obtain the xanthone compound. The 5 alpha-reductase activity test on the xanthone compound shows that the xanthone compound has relatively good inhibition effect on the 5 alpha-reductase and the compound can be used as a lead compound for preparing a 5 alpha-reductase inhibitor.

The invention relates to a color development method for detecting the soil hydroxylamine reductase activity, in particular to a method that the extracting solution after the soil culture is added in with the ferric iron salt solution and the Alfa-Alfa'bipyridyl solution and has the color developed in the subacidity environment, and then the hydroxylamine concentration of the extracting solution are colorimetric-determined by a spectrophotometric method. The hydroxylamine concentration of the culture solution is acquired by comparing the extinction value of the extracting solution with the standard curve; as a result, the soil hydroxylamine reductase activity is worked out. The color reaction is carried out at the normal temperature, the acquired solution shows orange, and the color comparison wave length is 520nm. If the color comparison can not be completed in 40 minutes, the masking agent should be added in order to prevent the side reaction. Compared with the traditional color development method, the improved color development method has the advantages of high sensitivity, small system error, high accuracy and precision and little use level of the color developing agent, more accurate and reliable analysis result and better analysis reproducibility.

The invention discloses arabidopsis thaliana gamma-INF induced lysosome thiol reductase gene and application thereof. The arabidopsis thaliana gamma-INF induced lysosome thiol reductase has a DNA sequence as shown in SEQ ID NO.1, and the expression protein of the reductase has an amino acid sequence as shown in SEQ ID NO.2. According to the reductase, the cDNA complete sequence of the gamma-INF induced lysosome thiol reductase gene is cloned from plant arabidopsis thaliana, and lysosome thiol reductase protein is successfully expressed, the thiol reductase activity of the in-vitro expressed arabidopsis thaliana gamma-INF induced lysosome thiol reductase for human IgG is detected by adopting an in-vitro thiol protease activity detection method, and the result indicates that the protein has strong thiol reductase activity and can have wide application in the field of plant immune researches.

The invention discloses a method for preparing alkyl glycoside, and belongs to the technical field of fine chemical engineering. The method comprises the steps that bacillus is firstly cultured, bacillus strains are obtained, then a certain number of bacillus strains are moved for ultraviolet mutagenesis, the strains with high reductase activity of acyl coenzymes A obtained after mutagenesis and high fatty alcohol productivity are selected for culture, and then fermentation is carried out; after fermentation is finished, glucose is added into the fermentation broth, and reacting is carried out to generate alkyl glycoside under the action of aldolase. According to the method, the aldolase is adopted as a catalyst, dehydration condensation between the glucose and dehydration of the glucose are avoided, the reaction time is shortened by 12 h through the efficient catalyzing performance of enzymes, the conversion rate and the yield are increased, the yield is increased by 95% or above, and the purity of the alkyl glycoside reaches 96%. The method is easy and convenient to operate, no organic reagent is adopted, environment friendliness is achieved, pollution is avoided, and industrial production is promoted.

The present invention relates to a gene for an enzyme involving in the synthesis of GDP-L-fucose. Particularly, the present invention relates to a GDP-4-keto-6-deoxy-D-mannose-3, 5-epimerase-4-reductase gene derived from Arabidopsis thaliana, and a process for producing GDP-L-fucose using the gene. An enzyme encoded by the gene is (a) a protein comprising an amino acid sequence represented by SEQ ID NO: 1; or (b) a protein comprising an amino acid sequence derived from the amino acid sequence of SEQ ID NO: 1 by deletion, substitution, addition or insertion of one or several amino acid residues, and having GDP-4-keto-6-deoxy-D-mannose-3, 5-epimerase-4-reductase activity. The present invention enables efficient mass production of GDP-L-fucose which is essential in performing addition of fucose, which has a very important function in sugar chains.

The invention relates to a modified microorganism for the production of PDO from a carbon substrate wherein the microorganism includes a three-step metabolic pathway including a first step of conversion of 2,4-dihydroxybutyrate (DHB) to obtain 2-oxo-4-hydroxybutyrate (OHB) by an enzyme having 2,4-DHB dehydrogenase activity, a second step of decarboxylation of the OHB to obtain 3-hydroxypropionaldehyde by an enzyme having 2-oxo-4-hydroxybutyrate decarboxylase activity, and a third step of reduction of the obtained 3-hydroxypropionaldehyde to obtain PDO with an enzyme having 3-hydroxypropionaldehyde reductase activity and the genes enabling the microorganism for the synthesis of DHB.

The present invention discloses a xanthomonadins synthesis related gene XC_4092. A gene sequence is as shown in SEQ ID NO.1. An encoded product has a 3-ketoacyl-ACP reductase activity and can catalyzea reduction reaction in xanthomonadins synthesis. The present invention further discloses an application of the gene XC_4092 in production of the colorless xanthan gum, xanthomonas campestris pathogenic strains of wild strains Xcc8004 are used as starting strains, the gene XC_4092 in a Xcc8004 genome is inactivated to complete gene modification to construct the colorless xanthan gum genetically engineered bacteria. Compared with the Xcc8004, the genetically engineered bacteria cannot synthesize xanthomonadins during fermentation processes, effectively improve appearance color of xanthan gum,and thus greatly improve quality of the xanthan gum product.

The invention relates to an isolated polypeptide having NADH dependent HMF reductase activity, wherein said polypeptide shows 80% homology to the amino acid sequence shown in SEQ ID NO:2 and which differs from SEQ ID NO:2 in that at least S117L and Y295 or S110 is substituted, a nucleotide sequence coding for said polypeptide, a vector comprising said polypeptide or nucleotide sequence, host comprising said nucleotide sequence or vector as well as the use of the polypeptide for the reduction of furan or carbonyl compounds in lignocellulosic material or in any furan or carbonyl containing material.

The invention relates to a modified microorganism for the production of PDO from a carbon substrate wherein the microorganism includes a three-step metabolic pathway including a first step of conversion of 2,4-dihydroxybutyrate (DHB) to obtain 2-oxo-4-hydroxybutyrate (OHB) by an enzyme having 2,4-DHB dehydrogenase activity, a second step of decarboxylation of the OHB to obtain 3-hydroxypropionaldehyde by an enzyme having 2-oxo-4-hydroxybutyrate decarboxylase activity, and a third step of reduction of the obtained 3-hydroxypropionaldehyde to obtain PDO with an enzyme having 3-hydroxypropionaldehyde reductase activity and the genes enabling the microorganism for the synthesis of DHB.

The present invention concerns a recombinant strain belonging to the order of Actinomycetales, wherein at least one gene encoding an enzyme having vanillin reductase activity is non-functional. The present invention is also related to a process for producing vanillin or a precursor thereof, comprising the culture of a recombinant strain in an appropriate medium comprising a substrate, and recovery of the produced vanillin or precursor thereof.

The present invention relates to a host cell having an elevated expression or activity of an enzyme as compared with the parent cell from which it has been derived, said enzyme having lactoyl-CoA reductase activity. Furthermore, provided is a method of producing lactaldehyde and / or 1,2-propanediol, said method comprising culturing said host cell and / or utilizing said enzyme to produce said compound.

Provided herein are recombinant yeast host cells that produce isobutanol and methods of their use. The provided yeast host cell comprises: isobutanol biosynthesis pathway and reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated at least one of the acetolactate reductase activity; or encoding has ketol acid reductoisomerase activity The heterologous polynucleotide of the polypeptide.

Enzymes that reduce specific metal or metalloid ions to insoluble form are important to science. Peptides isolated from yeast- and phage-display libraries can affect the size and morphology of inorganic materials during their synthesis. Herein, an Se binding peptide was fused to an enzyme capable of reducing selenite (SeO32−) to a Se0 nanoparticle (SeNP). The fusion of the Se binding peptide to the metalloid reductase provided size control of the resulting SeNP. The SeNP product also remains associated to the enzyme fusion. The Se binding peptide fusion to the enzyme increases the enzyme's SeO32− reductase activity. Modification of enzyme activity was absent, and the size control of particles was diminished when the Se binding peptide was added exogenously to the reaction mixture. Binding of the peptide is attributed to His based ligation that results in a conformational change to the peptide.

The invention discloses a protein coded by a medicago sativa L. anthocyanin reductase gene, wherein the protein has an amino acid sequence shown in SEQ ID No:2 or an amino acid sequence which is obtained through substituting, deleting or adding one or a plurality of amino acid residues in the amino acid sequence shown in SEQ ID No:2 and still has anthocyanin reductase activity. The invention further relates to a coded gene of the protein, an expression vector and a cell which contain the gene, a method for culturing medicago sativa L. with high tannin content and application of the coded gene of the protein, the expression vector and the cell. Through the expression of the medicago sativa L. anthocyanin reductase gene (MsBAN) provided by the invention in the medicago sativa L., the tannin content of the medicago sativa L. can be obviously increased. The discovery of the gene provides a gene resource for overcoming the problem that main grass plants (in particular leguminous plants, such as the medicago sativa L.) easily cause livestock to suffer from tympanites, and plays an important role in the research of genetic engineering improved grasses.

The present invention discloses a drug activator carrier comprising: a) particles having a metallic or metallic oxide core prepared from a paramagnetic material, said metallic core being coated with a coating material selected from polymer, metal or metal oxide; b) a biological material, having reductase activity, bound onto the metal coating the particles of step a), and wherein said biological material is capable of activating non-toxic pro-drugs into active and toxic drugs suitable for treating a disease; said drug activator carrier allowing targeted delivery of the toxic drug.

The application discloses a shampoo and conditioner composition for preventing hair loss. The composition contains saw palmetto fruit extract and lemongrass essential oil as active ingredients. Among them, saw palmetto fruit extract can effectively inhibit the activity of 5Alpha-reductase, lemongrass essential oil can effectively inhibit Malassezia furfur which causes dandruff, and the composition reduces hair loss from two aspects. The composition can effectively prevent or reduce hair loss.

The invention provides a method for detecting dihyaropteridine reductase activity, a detection kit and application thereof. According to the method for detecting dihyaropteridine reductase activity, provided by the invention, the pretreatment step is simple, the sample usage amount is reduced, a 5mm blood stain is changed into two 3mm blood stains, and the actual clinical application is promoted; due to advanced split charging and preheating of the buffer solution, the detection time of each sample is shortened to 10min, and the detection throughput is greatly improved; due to the improved sample pretreatment in the method provided by the invention, the method is stable, the enzymatic activity curve is smooth, the RSD (Relative Standard Deviation) of within-run precision of quantitative results is less than or equal to 3.37%, the RSD of between-run precision is less than or equal to 7.82%, the reproducibility and accuracy of quantification are greatly improved, the detection time is short, the detection throughput is improved, and the detection requirements on clinical actual samples are met.

A dehydrogenase mutant L283V / L286V and its preparation method and application relate to the field of biomedicine technology; the amino acid sequence of the mutant L283V / L286V is as shown in SEQ ID NO: 1; the amino acid sequence is as shown in SEQ ID NO: 1 The leucine at the 283rd position and the 286th position of the dehydrogenase indicated by ID NO:3 is mutated to valine at the same time. The dehydrogenase mutant L283V / L286V exhibited high selectivity in catalyzing the reduction of mesminin to S-nornicotine in the whole cell system, and had relatively high dehydrogenase and imine reductase activities at the same time, and the enzyme reduced Short time, high conversion rate. The product S-nornicotine obtained from the reaction has extremely high optical purity, which reduces the difficulty of subsequent purification work.