11 results about "Nornicotine" patented technology

Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) in tobacco plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for a root-specific nicotine demethylases, CYP82E10, and variants thereof, that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Compositions of the invention also include tobacco plants, or plant parts thereof, comprising a mutation in a gene encoding a CYP82E10 nicotine demethylase, wherein the mutation results in reduced expression or function of the CYP82E10 nicotine demethylase. Seed of these tobacco plants, or progeny thereof, and tobacco products prepared from the tobacco plants of the invention, or from plant parts or progeny thereof, are also provided. Methods for reducing the level of nornicotine, or reducing the rate of conversion of nicotine to nornicotine, in a tobacco plant, or plant part thereof are also provided. The methods comprise introducing into the genome of a tobacco plant a mutation within at least one allele of each of at least three nicotine demethylase genes, wherein the mutation reduces expression of the nicotine demethylase gene, and wherein a first of these nicotine demethylase genes encodes a root-specific nicotine demethylase involved in the metabolic conversion of nicotine to nornicotine in a tobacco plant or a plant part thereof. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

A method of decreasing conversion of nicotine to nornicotine is provided herein. The methods includes administering at least one basic region / leucine zipper (bZIP) type transcription factor inhibitor to an organism in need thereof. Also provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a bZIP type transcription factor binding site on a promoter of a nicotine N-demethylase (NND). Further provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a plant genome to knockout at least one bZIP type transcription factor.

The present application relates to the field of biotechnology, in particular to a tobacco NtClpR4 gene and application. The present application clones a nicotine transformation related gene NtClpR4 from tobacco for the first time, which encodes an ATP-dependent tyrosine protease Clp related subunit, the amino acid sequence is shown as SEQ ID NO: 2, and the nucleotide sequence is shown as SEQ ID NO: 1; the gene product affects the synthesis of nornicotine. After mutation of the NtClpR4 gene, the nicotine content of cured tobacco leaves decreases, and the nornicotine content increases, which provides a new technical means for nicotine transformation regulation and tobacco breeding.

Provided are a method for producing a low-reversion type tobacco plant line having low nornicotine content without using genetic engineering and artificial mutagenesis techniques, and a tobacco plant. The method according to the present invention comprises identifying a variety-specific DNA sequence linked to the low-reversion type and low nornicotine traits and making selection with the use of this sequence as an indicator.

The utility model relates to the technical field of demethylated arecoline detection, and discloses a reagent titration device for demethylated arecoline detection containing betel nut Chinese patent medicine, which comprises a bearing frame, the left side of the bearing frame is fixedly connected with a bearing plate, the upper side of the bearing plate is fixedly connected with a liquid storage cylinder, the upper side of the liquid storage cylinder is fixedly connected with a connecting assembly, and the connecting assembly is fixedly connected with the liquid storage cylinder. The connecting assembly is used for conveniently adding raw materials, a supporting frame is fixedly connected to the lower side of the bearing plate, a motor is fixedly connected to the interior of the supporting frame, a rotating shaft is fixedly connected to the output end of the motor, and a fixing sleeve is fixedly connected to the exterior of the rotating shaft; and fixing blocks which are uniformly distributed are fixedly connected to the outer part of the fixing sleeve. According to the device disclosed by the utility model, raw materials in the liquid storage cylinder can be fully and uniformly stirred, an experimenter does not need to manually shake a reagent bottle, and the dropping speed of a stock solution can be conveniently and accurately controlled to drop a standard solution.

The invention provides a method for predicting the early nicotine conversion rate of tobacco nicotine transformed plants based on a hyperspectral technology and application, and belongs to the technical field of agricultural information technologies and plant physiology. The method is used for solving the technical problems of hysteresis, destructiveness and low efficiency caused by the traditional method for determining the nicotine conversion rate and screening the nicotine transformant by a pure chemical method. The identification method comprises the following steps: acquiring hyperspectral data of a to-be-detected tobacco plant, inputting the hyperspectral data of the to-be-detected tobacco plant into a tobacco nicotine transformed plant early-stage identification model, predicting the content of nicotine and nornicotine, calculating the nicotine transformation rate, and judging the type of the to-be-detected tobacco plant according to the nicotine transformation rate. Based on the combination of hyperspectral remote sensing and machine learning, the nicotine and nornicotine contents of the tobacco plants are predicted, and the nicotine conversion rate is rapidly determined, so that the rapid, accurate and nondestructive early screening method for the tobacco nicotine transformed plants is realized.

The invention discloses application of a cinnabar tobacco glandular hair specific gene in acylated demethylated nicotine synthesis and aroma improvement and a method, and belongs to the fields of biochemistry, molecular biology and agricultural application. The specific gene is NtNAT1-NtNAT4, the nucleotide sequences of the specific gene are respectively shown as SEQ ID NO.1-4, the gene is highly expressed in glandular hair, prokaryotic expression and enzymatic activity analysis verify that the gene has acyltransferase activity, and jasmonic acid methyl ester can efficiently induce the expression of the gene. The gene participates in tobacco nicotine metabolism, and the encoded protein of the gene can catalyze acylation of demethylated nicotine, increase the accumulation amount of acylated demethylated nicotine and improve the tobacco fragrance. The invention provides a method for improving the tobacco aroma, and the method has important application value in tobacco modification and breeding.

The present invention relates to a method of reducing the content of at least one tobacco specific nitrosamine (TSNA) or a precursor of a TSNA in tobacco comprising expressing a deregulated cation efflux protein in a tobacco plant, or plant part thereof, or plant cell.

The invention provides coral hairy sporidium, a fungicide, a preparation method and application, and belongs to the technical field of microbial fermentation and tobacco processing. The preservation number of the coral coremiforme SC-11 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 34235, and the preservation number of the coral coremiforme SC-11 is CGMCC No. 34235. The provided strain can increase the content of carotenoid degradation products, Maillard reaction products, chlorophyll degradation products, terpenoids and the like, and increase the total content of aroma components of tobacco leaves; nicotine and derivatives (nornicotine, dehydroneonicotine and the like) of nicotine can be degraded, the content of alkaloid substances can be effectively reduced, and smoke irritation is reduced; the metabolite can reconstruct the aroma characteristics of cigar tobacco leaves, highlight the multiple aroma levels of honey sweet aroma, resin aroma, flower aroma, coffee aroma and the like, and remarkably improve the soft feeling and fineness of smoke at the same time.

The application belongs to the technical field of organic synthesis, and particularly relates to a preparation method of nicotine. The preparation method of the nicotine comprises the following steps: 3-bromopyridine and N-tert-butoxycarbonyl-proline are used as raw materials to photocatalytically synthesize an intermediate N-tert-butoxycarbonyl-nor-nicotine; then N-tert-butoxycarbonyl-nor-nicotine is reacted with trifluoroacetic acid to remove the tert-butoxycarbonyl group, and nor-nicotine is synthesized; finally, the nor-nicotine is subjected to a methylation reaction to synthesize nicotine. The preparation method of the nicotine has a short synthesis route, is simple to operate, has high yield, is low in cost, and is suitable for industrialized scale production.

Provided is a method for producing a low reversion type low nornicotine content tobacco plant line without use of a genetic modification technique or a factitious mutant preparation technique. Also provided is a tobacco plant. In the method in accordance with the present invention, a variety-specific DNA sequence that links with a low reversion type low nornicotine character is determined, and selection is carried out with use of the sequence as an index.