86 results about "Tobacco-specific nitrosamines" patented technology

The present invention relates to methods for hindering formation of tobacco-specific nitrosamines during processing of dark fire tobacco, as well as a facility in which at least portions of these methods may be conducted. According to the present invention, dark fire tobacco that has been harvested and that is generally green and/or yellow is exposed to an uncontrolled, yet active, ambient airflow so as to provide a substantially aerobic environment about the tobacco. This exposure of the dark fire tobacco to the ambient airflow may be done until the tobacco is substantially brown and/or substantially free of enzymatic activity. Subsequently, the tobacco is exposed to gaseous emissions (e.g., smoke) from combusting sawdust/wood. This step may be conducted at least until the tobacco exhibits a moisture content of no more than about 16% and/or until the tobacco exhibits a gloss or shine on a surface of the tobacco.

The present invention generally relates to tobacco and tobacco products having a reduced amount of nicotine and/or tobacco specific nitrosamines (TSNA). More specifically, several ways to make tobacco plants that have reduced nicotine and TSNA levels have been discovered. Embodiments include tobacco harvested from said tobacco plants, cured tobacco from said tobacco plants, tobacco products made with said cured tobacco and methods of making these compositions.

A class of molecularly imprinted polymers that specifically recognizes and binds to TSNAs are useful, for example, in analysis and separation of TSNAs from biological fluids. Such polymers are also useful in methods of treating and manufacturing tobacco products and materials.

A smoking article having one or more reconstituted tobacco split inner wrap strips is described. One or more split inner wrap strips extend coaxially along the tobacco column between the inner surface of an outer wrap of cigarette paper and the tobacco column. The tobacco blend includes burley and a second tobacco, such as flue-cured, oriental, Maryland, or rare and exotic tobaccos, and combinations thereof. This blend improves burley smoke character without increasing Hoffman analyte levels, especially tobacco-specific nitrosamines (i.e., TNSAs). A cigarette with an improved burley smoke character without increasing Hoffman analyte levels is also described.

The disclosed invention relates to visual content indicators of a tobacco product. More specifically, the invention includes labeling systems that provide at least one visual indicator of a tobacco product, methods for using the systems, and tobacco products that display one or more visual indicators of content. The present invention generally relates to tobacco products, which have a reduced amount of nicotine and tobacco specific nitrosamines (TSNAs), and contain one or more nicotine substitutes. The use of these tobacco products to reduce the exposure of an individual to nicotine, as well as, facilitating tobacco-use cessation is disclosed.

A method for preparing a tobacco flavor extract from cured tobacco materials. The tobacco flavor extract has reduced level of tobacco specific nitrosamines (TSNAs) and/or nicotine. The tobacco flavor extract is suitable for use in an electronic smoking device to provide a tobacco taste for a user of the electronic smoking device. The method preserves volatile and semi-volatile flavor components that can be released during a low-temperature vaporization process occurring in the electronic smoking device.

The invention relates to a method for measuring tobacco-specific nitrosamines (TSNAs for short) by using a super efficient liquid chromatogram-tandem mass spectrum combined method, and belongs to a method for detecting TSNAs. The method comprises the following steps of: adding internal standard-containing aqueous solution of ammonium acetate into a filter sheet after trapping smoke and cigarette filters smoked completely under standard conditions or tobacco shreds, performing ultrasonic extraction, transferring 1 to 2mL of extract into a small solid-phase extraction column taking N-vinyl pyrrolidone-benzene sulfonic acid copolymer as filler, flushing the small column by using aqueous solution of methanoic acid and methanol, finally eluting the small column by using solution of aqueous ammonia and methanol solution, analyzing the eluant by using an ultra performance liquid chromatography-mass spectrometer/mass spectrometer (UPLC-MS/MS), and quantifying according to the area ratio of a component peak to an isotope internal standard peak. The detecting method can be used for detecting the TSNAs in cured and hybrid cigarette mainstream smoke, cigarette side-stream smoke, filter tip intercepting smoke and tobacco shreds, and has the advantages of simple pretreatment, accurate quantification, high instrument analysis speed, low detection limit, high sample treatment flux and the like.

Tobaccos are cured in a manner so as to provide tobaccos having extremely low tobacco specific nitrosamine (TSNA) contents. Harvested Virginia tobacco is subjected to flue-curing so as to provide flue-cured tobacco. During the curing processing steps, contact of the tobacco with nitric oxide gases, such as those produced as combustion products of propane burning heating units, is avoided. Tobacco in curing barns is not subjected to direct-fire curing techniques, but rather, heat for tobacco curing can be provided by heat exchange or electrical heating methods.

The present invention relates to liquid phase chromatography-tandem mass spectrometry process for detecting specific tobacco fume nitrosamine content. The detection process includes: trapping tobacco fume with filtering sheet, adding internal standard substances, vibration extracting the filtering sheet with citric acid-phosphate buffering solution and cyclohexane, concentrating the organic phase extracted liquid in N2 protection, acidifying with hydrochloric acid, phase separating, neutralizing water phase with alkali solution, transferring the water phase solution into reverse chemical bonded solid phase extracting column, leaching with polar organic solvent aqua, eluting with polar organic solvent aqua, concentrating elutent, and analyzing in LC/MS/MS instrument quantitatively based on the area ratio between the component peak and the internal standard substance peak. The present invention has high repeatability, high recovering rate and low detection limit.

The invention relates to a method for measuring the contents of four tobacco-specific nitrosamines (TSNAs) in cigarette main stream smoke. The method measures the contents of four tobacco-specific nitrosamines in cigarette main stream smoke by triple quadrupole gas chromatography (GC)/mass spectrometry (MS), and measures the contents of the following four tobacco-specific nitrosamines in cigarette main stream smoke by internal standard method: NNN (N-nitrosonornicotine), NNK (4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone), NAT (N-nitrosoanatabine) and NAB (N-nitrosoanabasine). The method overcomes the defects of the conventional methods for detecting the standards related to four tobacco-specific nitrosamines (TSNAs) in cigarette main stream smoke, such as complicated operation and long time duration. Besides, the method has the advantages of high sensitivity and accuracy and good repeatability; and is applied to qualitative or quantitative measurement of four tobacco-specific nitrosamines (TSNAs) in cigarette main stream smoke for technical personnel in the tobacco industry.

The present invention relates to methods for hindering formation of tobacco-specific nitrosamines during processing of dark fire tobacco, as well as a facility in which at least portions of these methods may be conducted. According to the present invention, dark fire tobacco that has been harvested and that is generally green and/or yellow is exposed to an uncontrolled, yet active, ambient airflow so as to provide a substantially aerobic environment about the tobacco. This exposure of the dark fire tobacco to the ambient airflow may be done until the tobacco is substantially brown and/or substantially free of enzymatic activity. Subsequently, the tobacco is exposed to gaseous emissions (e.g., smoke) from combusting sawdust/wood. This step may be conducted at least until the tobacco exhibits a moisture content of no more than about 16% and/or until the tobacco exhibits a gloss or shine on a surface of the tobacco.

The invention discloses a method for reducing the TSNA (tobacco-specific nitrosamines) content and the nitrate content of a reconstituted tobacco concentrated solution according to an electrodialysis method. The method comprises three times of electrodialysis treatment procedures: for the first time, electrodialysis desalination treatment is carried out to remove nitrate radical in the reconstituted tobacco concentrated solution, wherein the acid consumption used for adjusting a feed liquid to be acidic is reduced at the same time; for the second time, electrodialysis desalination treatment is carried out under a strong acid condition to remove TSNA in the papermaking reconstituted tobacco concentrated solution; for the third time, electrodialysis desalination treatment is carried out to recycle nicotine, wherein by-products generated in the step can be used for preparing fertilizer. The method has the advantages that the characteristics of reconstituted tobacco concentrated solution and electrodialysis are utilized comprehensively; TSNA and the precursor-nitrate of TSNA are effectively removed, while great loss of nicotine caused by the conventional electrodialysis treatment is avoided; the by-products are utilized effectively; the effects in the aspects of efficiency improvement, energy conservation, pollution reduction and the like are obvious; the method is suitable for industrialized mass production.

The invention provides a preparation method of FeOOH nanometer materials. By preparing a solution A and a solution B, the solution A is used alone or in combination with the solution B at a certain ratio to respectively prepare different-crystal-phase FeOOH nanometer materials under a certain condition, namely, alpha-FeOOH, beta-FeOOH and delta-FeOOH. The invention further discloses a cigarette filter tip added with any one of the different-crystal-phase FeOOH nanometer materials. By adding the different-crystal-phase FeOOH nanometer materials prepared by the preparation method, which is disclosed by the invention, into the cigarette filter tip, the release amount of tobacco-specific nitrosamine in cigarette mainstream smoke can be effectively decreased. The additives for the cigarette filter tip are safe and non-toxic and low in cost and the preparation method is simple and high in yield and is suitable for industrial production.

The invention discloses a liquid chromatogram tandem mass spectrum measurement method for tobacco-specific nitrosamines in electronic cigarette liquid. The liquid chromatogram tandem mass spectrum measurement method is characterized by comprising the step of directly introducing a liquid chromatogram-electrospray ionization-tandem mass spectrometer for measurement after the electronic cigarette liquid is diluted so as to quantify an analyte by adopting an internal standard method. The content of the tobacco-specific nitrosamines in the electronic cigarette liquid can be rapidly and accurately detected. According to the liquid chromatogram tandem mass spectrum measurement method for tobacco-specific nitrosamines in the electronic cigarette liquid, the selectivity and the accuracy of the method are well improved by using a tandem mass spectrography, quantification limits of NNK (Nicotine-derived Nitrosamine Ketone), NNN (N'-Nitrosonornicotine), NAT (N-Nitrosoanatabine) and NAB (N-Nitrosoanabasine) are respectively 0.08ng/mL, 0.10ng/mL, 0.12ng/mL and 0.09ng/mL, the recovery rate range of the method is 90.9-108.1 percent, and the RSD (Relative Standard Deviation) is less than 12.1 percent. By selecting and optimizing a chromatographic column and an elution condition, the chromatographic separation process is well improved, and the chromatographic analysis time is shortened to be 6min.

The invention relates to a method for detecting release condition of tobacco specific nitrosamines (TSNAs) in a buccal tobacco product. The method is characterized in that: the release condition of the TSNAs in the buccal tobacco product in a human oral cavity is simulated, and release quantity and release speed are quantified timely, so that the in-vitro release speed and release degree of the TSNAs in the buccal tobacco product are detected. TSNAs release collection is realized by an in-vitro release collecting device which can simulate the release process of the TSNAs in the product in the human oral cavity and can collect TSNAs release liquid on line; and the TSNAs content of the release liquid is detected by a liquid chromatogram-electrospray tandem mass spectrum combined technique. The method has the advantages of simulating the release condition of the TSNAs in the oral tobacco product in the human oral cavity, detecting the release speed and the release degree of the TSNAs in various oral tobacco products quickly and accurately, effectively distinguishing release difference of the TSNAs in different products and contributing to popularization and application, along with high repeatability.

The invention relates to a method for detecting four tobacco-specific nitrosamines (TSNAs) in lateral exhaust gas of cigarettes by virtue of gas chromatography-tandem mass spectrometry. The method is characterized by comprising the steps of capturing the lateral exhaust gas of the cigarettes by virtue of a lateral fishtail cover preprocessed by an ascorbic acid solution and a Cambridge filter preprocessed by an ascorbic acid saturated methanol solution, washing the lateral fishtail cover with a hydrochloric acid solution, adding the washed hydrochloric acid solution and an TSNAs internal standard solution into a container in which the Cambridge filter is collected, extracting the Cambridge filter, processing extraction solution by virtue of a solid phase extraction column to obtain an eluate, drying the eluate with nitrogen, redissolving by virtue of dichloromethane to obtain a sample, and analyzing the sample by virtue of the gas chromatography-tandem mass spectrometry (GC-MS/MS). According to the method, the disadvantages of tedious preprocessing process, low detection sensitivity and strong matrix effect in an existing method are overcome, the preprocessing step of the sample is simplified, and the consumption of organic solvents is reduced; the method has the advantages that the detection is accurate, the sensitivity is high, and the repeatability is good.

A process for producing a reduced tobacco-specific nitrosamine (TSNA) tobacco plant comprising reducing and/or eliminating anatabine biosynthesis in wild-type tobacco plant. In addition, use of such plants for discovery of molecular markers that are closely linked with genes required for anatabine biosynthesis and for discovery of genes required for anatabine biosynthesis. In addition, a smoking composition, a smoking article and a smokeless tobacco oral delivery product contain the tobacco material.